KR101636058B1 - Composition for production of tagatose from fructose using enzyme combination reaction with substrates and uses thereof - Google Patents

Composition for production of tagatose from fructose using enzyme combination reaction with substrates and uses thereof Download PDF

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KR101636058B1
KR101636058B1 KR1020130103657A KR20130103657A KR101636058B1 KR 101636058 B1 KR101636058 B1 KR 101636058B1 KR 1020130103657 A KR1020130103657 A KR 1020130103657A KR 20130103657 A KR20130103657 A KR 20130103657A KR 101636058 B1 KR101636058 B1 KR 101636058B1
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신홍식
장승환
이상왕
백민석
백진호
김희정
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Abstract

본 발명은 프럭토스(fructose)에 헥소카이네이즈 또는 프럭토카이네이즈; 프럭토스 1,6-비스포스페이트 알돌레이즈; 및 타가토스-6-포스페이트 피타아제;를 첨가하여 프럭토스(fructose)에서 타가토스(tagatose)를 생산하는 방법에 관한 것으로, 본 발명의 타가토스 생산 방법은 새로운 효소를 이용하는 방법으로 환경친화적이고, 단가가 낮은 기질을 사용하며, 효소의 활성을 장기간 유지시킬 수 있으므로, 타가토스를 대량 생산하는데 효율적으로 사용될 수 있다.The present invention relates to a pharmaceutical composition comprising fructose, hexokinase or fructokinase; Fructose 1,6-bisphosphate aldolase; And tagatose-6-phosphate phytase to produce tagatose from fructose. The method for producing tagatose of the present invention is a method using a new enzyme, and is environmentally friendly, Since a substrate having a low unit cost is used and the activity of the enzyme can be maintained for a long time, it can be efficiently used for mass production of tagatose.

Description

기질 및 효소 컴비네이션 반응을 이용한 프럭토스로부터 타가토스 생산용 조성물 및 이의 용도{Composition for production of tagatose from fructose using enzyme combination reaction with substrates and uses thereof}TECHNICAL FIELD The present invention relates to a composition for producing tagatose from fructose using a substrate and an enzyme combination reaction and a use thereof.

본 발명은 프럭토스로부터 타가토스를 생산하기 위한 조성물 및 이의 용도에 관한 것으로, 상세하게는 (1) 프럭토스를 인산화시키는 프럭토카이네이즈 또는 헥소카이네이즈, (2) 프럭토스 6-포스페이트(fructose 6-phosphate)를 타가토스 6-포스페이트(tagatose 6-phosphate)로 이성화시키는 프럭토스 1,6-비스포스페이트 알돌레이즈 및 (3) 타가토스 6-포스페이트에서 인산기를 제거하는 타가토스-6-포스페이트 피타아제에 의한 고효율 타가토스 생산 방법 및 그 조성물에 관한 것이다.The present invention relates to a composition for producing tagatose from fructose and a use thereof. More particularly, the present invention relates to (1) fructokinase or hexokinase for phosphorylating fructose, (2) fructose 6- bisphosphate aldolase which isomerizes phosphate to tagatose 6-phosphate and (3) tagatose-6-phosphate phytase which removes phosphate groups from tagatose 6-phosphate. The present invention relates to a method for producing high-efficiency tagatose and a composition thereof.

타가토스(D-tagatose)는 단당류(6탄당)의 일종으로서, 프럭토스(D-fructose)의 C-4 에피머이고 갈락토스(D-galactose)의 이성질체(isomer)이다. 타가토스의 당도는 흔히 설탕이라 부르는 자당(수크로스, sucrose)의 92% 정도인 것으로 알려져 있으며, 칼로리 생성율은 자당(4 kcal/g)의 37.5% (1.5kcal/g) 정도로 낮은 것으로 알려져 있다. 흡습성 또한 수크로스와 비슷하게 낮으며, 온도 안정성은 식품제조와 보관 동안 적용되는 전형적인 온도에는 안정하지만, 다른 환원당들(reducing sugars) 처럼 온도 상승에 따라 카라멜화(caramelization)되는 특성이 있다.D-tagatose is a kind of monosaccharide (hexose), which is a C-4 epimer of D-fructose and an isomer of D-galactose. The sugar content of tagatose is known to be about 92% of sucrose, commonly called sugar, and the calorie production rate is known to be as low as 37.5% (1.5 kcal / g) of sucrose (4 kcal / g). Hygroscopicity is also low, similar to sucrose, and temperature stability is stable at typical temperatures applied during food preparation and storage, but caramelized with increasing temperature, like other reducing sugars.

타가토스는 기능성 감미료로서 음식, 음료 및 제약 제품의 설탕 대용으로 사용할 수 있는 천연 단당류로서, 항 당뇨 및 비만 조절 약물로서의 효능에 대한 시험 중에 있다.Tagatose is a natural sweetener that can be used as a sugar substitute for food, beverage and pharmaceutical products as a functional sweetener, and is being tested for its efficacy as an anti-diabetic and obesity-controlling drug.

자연계에서, 타가토스는 카카오나무(Sterculia setigera)의 검 삼출물(gum exudate)의 한 성분으로 자연계에서 처음으로 확인되었으며, 지의류인 Rocella 종의 올리고당의 한 성분으로서도 검출되었다. 또한, 타가토스는 세균의 락토스 대사과정에서 L-아라비노오스 이성화효소의 이성화 작용 촉매로도 생성될 수 있다.In the natural world, Tagatos is a cacao tree ( Sterculia It was first identified in nature as a component of the gum exudate of setigera and was also detected as a component of the oligosaccharide of the lichen species Rocella . In addition, tagatose can also be produced as a catalyst for the isomerization of L-arabinose isomerase in the lactose metabolism of bacteria.

아쓰로박터 글로비포미스(Arthrobacter globiformis), 마이코박테리움 스메그마티스(Mycobacterium smegmatis), 엔테로박터 아글로메란스(Enterobacter agglomerans), 글루코노박터 옥시단스(Gluconobacter oxydans)와 같은 미생물을 이용하여 갈락티톨(D-galactitol)을 산화시켜서 타가토스를 생산할 수 있다. 이 경우 생체전환 최대 수율은 92%인 것으로 보고하였다. 그러나 갈락티톨의 원료로서의 사용불가능성과 높은 비용 때문에 대규모 타가 토스의 생산에는 사용되지 못하고 있다.Before the Miss Global ahsseuro bakteo (Arthrobacter globiformis), Mycobacterium Smeg Matisse (Mycobacterium galactitol may be produced by using microorganisms such as smegmatis , Enterobacter agglomerans , and Gluconobacter oxydans to produce tagatose. In this case, the maximum biodegradation yield was reported to be 92%. However, galactitol has not been used for the production of large-scale tagatose because of its unavailability as a raw material and high cost.

뮤코라세아(Mucoraceae) 곰팡이의 여러 변종을 이용한 생물전환법으로 사이코스(D-psicose)로부터 타가토스를 제조하는 방법이 제안되었다. 사이코스는 희귀한 당이지만, 슈도모나스 시코리(Pseudomonas cichorii)나 로도박터 스패로이데스(Rhodobacter sphaeroides)로부터의 D-타가토스 3-에피머레이즈 또는 아그로박테리움 튜머파시엔스(Agrobacterium tumefaciens)로부터의 D-사이코스 3-에피머레이즈를 사용하여 상대적으로 싼 원료인 프럭토스(D-fructose)을 에피머화하여 생산할 수 있다. 그러나 사이코스를 통해 프럭토스로부터 타가토스를 생산하는 방법은 상업적인 적용성은 매우 희박한 것으로 알려져 있다.A method of producing tagatose from D-psicose by a biotransformation method using several strains of Mucoraceae fungi has been proposed. Between courses per Although rare, Pseudomonas Francisco Li (Pseudomonas cichorii) or Rhodobacter seupaeroyi des D- tagatose 3-epi Murray's or Agrobacterium tyumeo Pacific Enschede from (Rhodobacter sphaeroides) (Agrobacterium tumefaciens) can produce a relatively cheap raw material, fructose (D-fructose) and D- between the use of 3-epi Murray's course from the epimerization. However, the commercial applicability of the method of producing tagatose from fructose via the Saikos is known to be very sparse.

타가토스는 열처리 유제품에서 온도상승으로 인해 락토스로부터 소량 생성될 수 있지만, β-D-갈락토시데이즈에 의한 유당(락토스, lactose)의 가순분해와 이 반응으로 생성된 글루코스와 갈락토스의 분리 및 갈락토스의 화학적 또는 효소적 이성화반응(isomerisation)으로 제조할 수 있다. 갈락토스는 L-아라비노오스 이성화효소에 의해 체외에서도 타가토스로의 변환이 촉진되며, 타가토스 대규모 생산을 위한 가장 유망한 효소로 알려져 있다.Tagatose can be produced in a small amount from lactose due to the temperature rise in heat-treated dairy products, but it is difficult to separate the lactose (lactose) by β-D-galactosidase and the glucose and galactose produced by this reaction, For example, by chemical or enzymatic isomerisation. Galactose is known to be the most promising enzyme for large-scale production of tagatose, which promotes the conversion of tagatose to extracellular enzymes by L-arabinose isomerase.

갈락토스 이성화는 타가토스의 생산을 위한 경제적 방법이다. 갈락토오스는 화학적 또는 효소 이성화할 수 있다. 타가토스의 생산을 위한 화학적 방법은 Biospherics Inc. (USA)에 의해 개발 및 특허되었다. 특허에 따르면, 수산화칼슘과 촉매로서 염화칼슘을 이용하여 갈락토오스를 이성화하여 타가토스를 생성할 수 있다고 보고했다. 수산화칼슘은 강 알칼리성 조건과 상대적으로 낮은 온도에서 타가토스와 불용성 복합체를 형성한다. 이러한 현탁액에 산, 바람직하게는 이산화탄소를 처리하면 복합체로부터 타가토스를 유리시킬 수 있다. 불용성 칼슘염은 여과과정을 통해 제거하고, 나머지 이온은 이온 교환 크로마토그래피를 이용해 제거할 수 있다. 이후 순수한 타가토스는 결정화를 통해 얻을 수 있다. 그러나 이 화학적 방법은 부산물 및 화학 폐기물 형성과 같은 몇 가지 단점을 가지고 있다. 이 방법에는 반응 혼합물의 집중적인 냉각을 필요로 하기 때문에 에너지 소비가 많은 공정이라는 단점을 가지고 있다.Galactose isomerization is an economical method for the production of tagatose. Galactose can be chemically or enzymatically isomerized. The chemical method for the production of tagatose is Biospherics Inc. (USA). According to the patent, it has been reported that galactose can be isomerized using calcium hydroxide and calcium chloride as a catalyst to generate tagatose. Calcium hydroxide forms insoluble complexes with tagatose at relatively low temperatures under strongly alkaline conditions. Treatment of this suspension with an acid, preferably carbon dioxide, can liberate tagatose from the complex. Insoluble calcium salts can be removed by filtration and the remaining ions can be removed using ion exchange chromatography. Since then, pure tagatos can be obtained through crystallization. However, this chemical method has some drawbacks such as by-products and chemical waste formation. This method has the disadvantage of energy-consuming processes because it requires intensive cooling of the reaction mixture.

갈락토오스에서 타가토스를 생산하는 효소 방법은 생체촉매로 L-아라비노스 이성화효소를 사용하는 방법이다. 대부분의 L-아라비노스 이성화효소는 최적활성을 보이는 온도가 60 내지 95℃로 매우 높고, 최적 pH도 7 이상이다. 더욱이, 대부분의 L-아라비노스 이성화효소가 고활성과 안정성을 위해서는 Mn2 + 또는 Co2 +와 같은 금속이온을 필요로 하고 있다. 산업적 적용을 위한 산성 pH와 저에너지 공정을 위한 낮은 반응 온도 및 Co2 +와 같은 식품적용 부적합한 이온 사용 등은 개선해야 할 문제점으로 지적되고 있다.An enzymatic method for producing tagatose from galactose is a method using L-arabinose isomerase as a biocatalyst. Most of the L-arabinose isomerase exhibits an optimum temperature of 60 to 95 DEG C and a pH of 7 or more. Furthermore, most of the L- arabinose isomerase to a high activity and stability and require metal ions such as Mn + 2 or Co + 2. Unsuitable for food application ion problem and the like can be improved using such a low reaction temperature and the Co 2 for the acidic pH and low energy process for the industrial application it has been pointed out +.

이와 같은 문제점을 해결하기 위해, 원료의 수급이 안정적이고 낮은 가격의 포도당이나 프럭토스를 기질로 하며, 화학적인 전환법이나 고비용 효소전환법이 아닌 저비용 효소전환법을 이용하여 타가토스를 생산하는 방법이 활발히 연구되고 있는 실정이다.In order to solve these problems, a method of producing tagatose by using a low-cost enzyme conversion method instead of a chemical conversion method or a high-cost enzyme conversion method using glucose or fructose as a substrate as a stable and low-cost raw material Is being studied actively.

현재, 단일 효소반응으로 프럭토스에서 타가토스를 생산하는 단일 효소반응은 알려져 있지 않다. 지금까지 알려진 에피머화 효소들의 메커니즘으로는 전환이 거의 일어나지 않고 생산수율이 현저히 낮은 것으로 알려져 있다.Currently, the single enzyme reaction that produces tagatose in fructose by a single enzyme reaction is not known. It is known that the mechanism of epimerization enzymes known so far is that there is little conversion and production yield is remarkably low.

한국등록특허 제0464061호에는 '아라비노스 이성화효소의 고정화에 의한 타가토스의 생산방법'이 개시되어 있고, 한국공개특허 제2008-0053708호에는 '높은 전환율의 갈락토스 이성화 반응을 이용한 타가토스의 제조방법'이 개시되어 있으나, 본 발명의 프럭토스를 기질로 하는 혼합 효소반응을 이용해 타가토스를 고효율로 제조하는 방법에 대해서는 기재된 바가 없다.Korean Patent No. 0464061 discloses 'a method for producing tagatose by immobilization of arabinose isomerase', Korean Patent Publication No. 2008-0053708 discloses a method for producing tagatose using galactose isomerization with high conversion rate However, there is no description about a method for producing tagatose with high efficiency using a mixed enzyme reaction using the fructose of the present invention as a substrate.

본 발명은 상기와 같은 요구에 의해 도출된 것으로서, 본 발명은 프럭토스를 기질로 하는 혼합 효소반응을 이용해 타가토스를 고효율로 제조하고자 한다. 구체적으로, 본 발명은 비교적 낮은 단가의 프럭토스를 프럭토스 6-포스페이트(fructose 6-phosphate)로 인산화시키는 프럭토카이네이즈(fructokinase) 또는 헥소카이네이즈(hexokinase), 프럭토스 6-포스페이트를 타가토스 6-포스페이트로 전환시키는 프럭토스 1,6-비스포스페이트 알돌레이즈(fructose 1,6-bisphosphate aldolase)와 타가토스 6-포스페이트로부터 인산기를 제거하는 피타아제(phytase) 효소를 혼합으로 이용하여 94% 이상의 높은 수율로 타가토스를 생산하는 기술이다.The present invention has been made in view of the above-mentioned needs, and an object of the present invention is to produce tagatose with high efficiency by using a mixed enzyme reaction using fructose as a substrate. Specifically, the present invention relates to fructokinase or hexokinase which phosphorylates a relatively low unit of fructose with fructose 6-phosphate, fructose 6-phosphate as a tagatose 6- Phosphatase 1,6-bisphosphate aldolase and phytase enzyme which removes phosphoric acid from tagatose 6-phosphate, which are converted into phosphate, in a high yield of 94% or more It is the technology that produces the rotagotose.

본 발명에서는 프럭토스로부터 타가토스를 효소반응을 이용해 생산하기 위하여, 프럭토스, 프럭토카이네이즈, 프럭토스 6-비스포스페이트 알돌레이즈 및 피타아제를 하나의 반응기에 혼합하여 반응시키는 컴비네션 반응 방법을 제공하고자 한다.In the present invention, in order to produce tagatose from fructose using an enzyme reaction, a complex reaction method in which fructose, fructokinase, fructose 6-bisphosphate aldolase, and phytase are mixed in one reactor and reacted .

또한, 본 발명에서는 생산비용절감을 위한 효소고정화 공정을 이용한 고효율 타가토스 생산방법을 제공하고, 여기에는 개별적으로 고정화된 프럭토카이네이즈, 프럭토스 6-비스포스페이트 알돌레이즈 및 피타아제를 순서적으로 통과시키는 공정으로 타가토스를 생산하는 방법을 제공하고자 한다.In addition, the present invention provides a method for producing high-efficiency tagatose using an enzyme immobilization process for reducing production cost, which comprises sequentially passing individually immobilized fructokinase, fructose 6-bisphosphate aldolase, and phytase To provide a method for producing tagatose.

또한, 타가토스의 안정적인 생산을 위하여, 실제 공업적으로 유리한 알지네이트 등을 사용한 포괄법으로 세포를 고정화시켜서, 가격이 저렴하고, 고정화 후 별도의 강도처리공정이 필요 없으며, 장기간 연속운전에도 안정한 제반 특성을 갖춘 타가토스 생산 공정을 제공하고자 한다.In addition, in order to stably produce tagatose, cells are immobilized by a comprehensive method using industrially advantageous alginate, so that the cost is low, a separate strength treatment process is not required after immobilization, and all the characteristics To provide a tagatose production process.

상기 과제를 해결하기 위해, 본 발명은 다양한 미생물 유래의 프럭토스 1,6-비스포스페이트 알돌레이즈 효소를 유효성분으로 함유하는 프럭토스(fructose)에서 타가토스(tagatose)를 생산하기 위한 조성물을 제공한다.In order to solve the above problems, the present invention provides a composition for producing tagatose from fructose containing, as an active ingredient, fructose 1,6-bisphosphate aldolase enzyme derived from various microorganisms .

또한, 본 발명은 프럭토스(fructose)에 헥소카이네이즈 또는 프럭토카이네이즈; 프럭토스 1,6-비스포스페이트 알돌레이즈; 및 타가토스-6-포스페이트 피타아제;를 첨가하여 반응시키는 단계를 포함하는 프럭토스(fructose)에서 타가토스(tagatose)를 생산하는 방법을 제공한다.The present invention also relates to a pharmaceutical composition comprising fructose, hexokinase or fructokinase; Fructose 1,6-bisphosphate aldolase; And adding tagatose-6-phosphate phytase to the fructose to produce a tagatose in fructose.

또한, 본 발명은 상기 방법에 의하여 생산된 타가토스를 유효성분으로 포함하는 식품 조성물을 제공한다.The present invention also provides a food composition comprising the tagatose produced by the above method as an active ingredient.

본 발명에 따른 타가토스 생산 방법은 미생물로부터 얻은 효소를 이용하므로 환경 친화적이며, 간단한 효소 고정화 과정만을 필요로 하고, 종래 사이코스 생산과 비교하여 기질에 있어서는 단가가 낮은 기질을 사용하고, 수율에 있어서는 현저하게 높기 때문에 생산 경비를 크게 줄이는 한편 생산효과를 극대화할 수 있는 효과가 있다.The method of producing tagatose according to the present invention uses an enzyme obtained from a microorganism, so it is environmentally friendly, requires only a simple enzyme immobilization process, uses a substrate having a low unit cost in comparison with the conventional production of a scissors, It is possible to greatly reduce the production cost and to maximize the production effect.

도 1은 본 발명에 기재한 칵테일 반응에 의한 프럭토스에서 타가토스로의 생산을 도식화한 것이다.
도 2는 본 발명에서 분리 정제한 3가지 효소인 헥소카이네이즈(1번 레인), 프럭토카이네이즈(2번 레인) 및 프럭토스 1,6-비스포스페이트 알돌레이즈(3번 내지 5번 레인)의 SDS-PAGE 결과이다.
도 3은 사카로마이세스 세레비지애(Saccharomyces cerevisiae) 유래 헥소카이네이즈와 5 mM에서 50 mM의 프럭토스를 1시간 동안 반응하여 생산된 프럭토스 6-포스페이트의 생산량을 나타낸 그래프이다.
도 4는 본 발명의 프럭토스 1,6-비스포스페이트 알돌레이즈의 금속 특이성(A), 최적 pH(B), 최적 온도(C) 및 반응 시간(D)에 따른 프럭토스 6-포스페이트에서 타가토스 6-포스페이트의 전환율을 나타낸 것이다.
도 5는 본 발명의 피타아제 농도에 따른 타가토스 6-포스페이트에서 타가 토스로의 전환율을 나타낸 것이다.
도 6은 프럭토카이네이즈와 프럭토스 1,6-비스포스페이트 알돌레이즈를 함께 클로닝할 목적으로 본 발명에서 사용한 duet vector 시스템을 도식화한 것이다.Figure 1 schematically illustrates the production of fructose to tagatose by the cocktail reaction described in the present invention.
FIG. 2 is a graph showing the results of SDS analysis of three enzymes separated and purified in the present invention, hexokanease (lane 1), fructokinase (lane 2) and fructose 1,6-bisphosphate aldolase (lanes 3 to 5) -PAGE The result.
FIG. 3 is a graph showing the activity of Saccharomyces < RTI ID = 0.0 > and the production of fructose 6-phosphate produced by reacting hexokinase derived from S. cerevisiae with 5 mM to 50 mM fructose for 1 hour.
4 is a graph showing the effect of fructose 6-phosphate on the metal specificity (A), optimum pH (B), optimum temperature (C) and reaction time (D) of fructose 1,6-bisphosphate aldolase of the present invention 6-phosphate. ≪ / RTI >
Figure 5 shows the conversion of tagatose 6-phosphate to tagatose according to the phytase concentration of the present invention.
Figure 6 is a schematic representation of the duet vector system used in the present invention for the purpose of cloning fructokinase and fructose 1,6-bisphosphate aldolase together.

본 발명의 목적을 달성하기 위하여, 본 발명은 알카니보락스(Alcanivorax), 바실러스(Bacillus), 비피도박테리움(Bifidobacterium), 코리네박테리움(Corynebacterium), 엔테로코커스(Enterococcus), 지오바실러스(Geobacillus), 락토바실러스(Lactobacillus), 마리노박터(Marinobacter), 메틸로바실러스(Methylobacillus), 리조비움(Rhizobium), 스태필로코커스(Staphylococcus), 스트렙토코커스(Streptococcus) 및 스트렙토마이세스(Streptomyces) 속 미생물로 이루어진 군으로부터 선택되는 미생물 유래의 프럭토스 1,6-비스포스페이트 알돌레이즈 효소를 유효성분으로 함유하는 프럭토스(fructose)에서 타가토스(tagatose)를 생산하기 위한 조성물을 제공한다.According to an aspect of the invention, the invention is seen Carney borax (Alcanivorax), Bacillus (Bacillus), Bifidobacterium (Bifidobacterium), Corynebacterium (Corynebacterium), Enterococcus (Enterococcus), geo bacilli ( Geobacillus , Lactobacillus , Marinobacter , Methylobacillus , Rhizobium , Staphylococcus , Streptococcus , and Streptomyces . A fructose derived from a microorganism selected from the group consisting of microorganisms belonging to the genus Escherichia. The present invention provides a composition for producing tagatose from fructose containing the fructose 1,6-bisphosphate aldolase enzyme as an active ingredient.

본 발명에서, 프럭토스 1,6-비스포스페이트 알돌레이즈는 프럭토스 1,6-비스포스파타아제(fructose 1,6-bisphosphatase)와 동일한 의미로 사용된다.In the present invention, fructose 1,6-bisphosphate aldolase has the same meaning as fructose 1,6-bisphosphatase.

본 발명의 일 구현예에 따른 조성물은 상기 프럭토스 1,6-비스포스페이트 알돌레이즈 효소 외에 헥소카이네이즈 또는 프럭토카이네이즈; 및 타가토스-6-포스페이트 피타아제;로 이루어진 군으로부터 선택되는 하나 이상의 효소를 추가로 포함할 수 있다. 즉, 본 발명의 조성물은 프럭토스 1,6-비스포스페이트 알돌레이즈 효소와 헥소카이네이즈 또는 프럭토카이네이즈를 포함할 수도 있고, 프럭토스 1,6-비스포스페이트 알돌레이즈 효소와 타가토스-6-포스페이트 피타아제를 포함할 수도 있고, 프럭토스 1,6-비스포스페이트 알돌레이즈 효소, 헥소카이네이즈 또는 프럭토카이네이즈 및 타가토스-6-포스페이트 피타아제를 포함할 수도 있다. 또한, 상기 타가토스-6-포스페이트 피타아제 대신에 타가토스-6-포스파타아제 효소를 추가로 포함할 수도 있다.The composition according to one embodiment of the present invention may further comprise, in addition to the fructose 1,6-bisphosphate aldolase enzyme, hexokinase or fructokinase; And a tagatose-6-phosphate phytase. That is, the composition of the present invention may contain a fructose 1,6-bisphosphate aldolase enzyme and hexokinase or fructokinase, and may contain a fructose 1,6-bisphosphate aldolase enzyme and a tagatose-6- , And may include fructose 1,6-bisphosphate aldolase enzyme, hexokinase or fructokainase, and tagatose-6-phosphate phytase. In addition, the tagatose-6-phosphatase may further include a tagatose-6-phosphatase enzyme instead of the tagatose-6-phosphate phytase.

본 발명의 일 구현예에 따른 조성물에서, 상기 프럭토스 1,6-비스포스페이트 알돌레이즈 효소는 알카니보락스 보르쿠멘시스(Alcanivorax borkumensis), 바실러스 알칼로필루스(Bacillus alcalophilus), 바실러스 아밀로리쿼파시엔스(Bacillus amyloliquefaciens), 바실러스 아조토포르만스(Bacillus azotoformans), 바실러스 셀룰로실리티쿠스(Bacillus cellulosilyticus), 바실러스 코아귤란스(Bacillus coagulans), 바실러스 사이토톡시쿠스(Bacillus cytotoxicus), 바실러스 할로두란스(Bacillus halodurans), 바실러스 리케니포르미스(Bacillus licheniformis), 바실러스 마카우엔시스(Bacillus macauensis), 바실러스 메가테리움(Bacillus megaterium), 바실러스 메타놀리쿠스(Bacillus methanolicus), 바실러스 닐소니(Bacillus nealsonii), 바실러스 슈도피르무스(Bacillus pseudofirmus), 바실러스 셀리니티레두센스(Bacillus selenitireducens), 바실러스 바이헨스테파넨시스(Bacillus weihenstephanensis), 비피도박테리움 브레베(Bifidobacterium breve), 코리네박테리움 글루타미쿰(Corynebacterium glutamicum), 코리네박테리움 크로펜스테티(Corynebacterium kroppenstedtii), 엔테로코커스 아시니(Enterococcus asini), 엔테로코커스 카캐(Enterococcus caccae), 엔테로코커스 카셀리플라부스(Enterococcus casseliflavus), 엔테로코커스 세코룸(Enterococcus cecorum), 엔테로코커스 콜룸배(Enterococcus columbae), 엔테로코커스 패칼리스(Enterococcus faecalis), 엔테로코커스 패시움(Enterococcus faecium), 엔테로코커스 플라베센스(Enterococcus flavescens), 엔테로코커스 길부스(Enterococcus gilvus), 엔테로코커스 해모페록시두스(Enterococcus haemoperoxidus), 엔테로코커스 이탈리쿠스(Enterococcus italicus), 엔테로코커스 모라비엔시스(Enterococcus moraviensis), 엔테로코커스 문티(Enterococcus mundtii), 엔테로코커스 팔렌스(Enterococcus pallens), 엔테로코커스 페니쿨리콜라(Enterococcus phoeniculicola), 엔테로코커스 라피노수스(Enterococcus raffinosus), 엔테로코커스 사카로리티쿠스(Enterococcus saccharolyticus), 엔테로코커스 종(Enterococcus sp .), 엔테로코커스 빌로룸(Enterococcus villorum), 지오바실러스 카우스토필루스(Geobacillus kaustophilus), 지오바실러스 종(Geobacillus sp.), 지오바실러스 스테아로써모필루스(Geobacillus stearothermophilus), 지오바실러스 써모데니트리피칸스(Geobacillus thermodenitrificans), 락토바실러스 애시도필루스(Lactobacillus acidophilus), 락토바실러스 아밀로리티쿠스(Lactobacillus amylolyticus), 락토바실러스 안트리(Lactobacillus antri), 락토바실러스 카제이(Lactobacillus casei), 락토바실러스 크리스파투스(Lactobacillus crispatus), 락토바실러스 커바투스(Lactobacillus curvatus), 락토바실러스 델브루에키 아종 불가르쿠스(Lactobacillus delbrueckii subsp . bulgaricus), 락토바실러스 헬베티쿠스(Lactobacillus helveticus), 락토바실러스 이너스(Lactobacillus iners), 락토바실러스 존슨아이(Lactobacillus johnsonii), 락토바실러스 뮤리누스(Lactobacillus murinus), 락토바실러스 파라카제이 아종 파라카제이(Lactobacillus paracasei subsp . paracasei), 락토바실러스 플란타룸 아종 플란타룸(Lactobacillus plantarum subsp . plantarum), 락토바실러스 루테리(Lactobacillus reuteri), 락토바실러스 람노수스(Lactobacillus rhamnosus), 락토바실러스 루미니스(Lactobacillus ruminis), 락토바실러스 살리바리우스(Lactobacillus salivarius), 락토바실러스 종(Lactobacillus sp .), 마리노박터 히드로카보노클라스티쿠스(Marinobacter hydrocarbonoclasticus), 메틸로바실러스 플라젤라투스(Methylobacillus flagellatus), 리조비움 그라하미(Rhizobium grahamii), 리조비움 레규미노사룸 비브이. 트리폴리아이(Rhizobium leguminosarum bv . trifolii), 리조비움 루피니(Rhizobium lupini), 리조비움 종(Rhizobium sp .), 스태필로코커스 에피더미디스(Staphylococcus epidermidis), 스트렙토코커스 써모필러스(Streptococcus thermophilus), 스트렙토마이세스 아베미틸리스(Streptomyces avermitilis) 및 스트렙토마이세스 비리도크로모게네스(Streptomyces viridochromogenes)로 이루어진 군으로부터 선택되는 미생물로부터 유래한 효소일 수 있으나, 이에 제한되지 않는다.In a composition according to an embodiment of the present invention, the fructose 1,6-bisphosphate aldolase enzyme is selected from the group consisting of Alcanivorax borkumensis), Bacillus know Phil sword Luce (Bacillus alcalophilus), Bacillus amyl Lori query Pacifico Enschede (Bacillus amyloliquefaciens), Bacillus azo Sat formate only switch (Bacillus azotoformans), silica tea kusu (Bacillus of the Bacillus cellulite cellulosilyticus), Bacillus core Tangerine Lance (Bacillus coagulans), Bacillus Saito ethoxy kusu (Bacillus cytotoxicus), Bacillus halo two lance (Bacillus halodurans), Bacillus Fort Lee Kenny Miss (Bacillus licheniformis), Macau N-Sys Bacillus (Bacillus macauensis), MEGATHERIUM Bacillus (Bacillus megaterium), Bacillus meth fun kusu (Bacillus methanolicus), Bacillus carbonyl Sony (Bacillus nealsonii , Bacillus pseudofirmus), Bacillus selrini Tire, two sense (Bacillus selenitireducens), Bacillus by Henderson seutepa cis norbornene (Bacillus weihenstephanensis), Bifidobacterium breve (Bifidobacterium breve), Corynebacterium glutamicum (Corynebacterium glutamicum ), Corynebacterium kroppenstedtii , Enterococcus asini ), enterococcus cata ( Enterococcus caccae), Enterococcus Kassel ripple La Booth (Enterococcus casseliflavus), Enterococcus three Corum (Enterococcus cecorum ), enterococcus colomba ( Enterococcus columbae ), Enterococcus faecalis faecalis ), Enterococcus sp. faecium , enterococcus flavosense flavescens , Enterococcus gilvus ), enterococcus halobacterium ( Enterococcus haemoperoxidus , Enterococcus italicus , Enterococcus moraviensis , Enterococcus, mundtii ), Enterococcus pallens), Enterococcus penny Cooley coke (Enterococcus phoeniculicola), Enterococcus raffinose Versus (Enterococcus raffinosus ), enterococcus saccharolyticus ( Enterococcus saccharolyticus), Enterococcus species (Enterococcus sp . ), Enterococcus villorum , Geobacillus kaustophilus), geo Bacillus species (Geobacillus sp.), loose a brush (Geobacillus as geo Bacillus stearate stearothermophilus ), Geobacillus thermodenitrificans ( Geobacillus thermodenitrificans ), Lactobacillus acidophilus ( Lactobacillus acidophilus , Lactobacillus amylolyticus amylolyticus , Lactobacillus antri , Lactobacillus < RTI ID = 0.0 > casei ), Lactobacillus crispatus , Lactobacillus < RTI ID = 0.0 > gt; Lactobacillus < / RTI > delbrueckii subsp . bulgaricus), Lactobacillus helveticus (Lactobacillus helveticus , Lactobacillus < RTI ID = 0.0 > iners , Lactobacillus johnsonii ), Lactobacillus murinus ), Lactobacillus paracasei paracasei ( Lactobacillus paracasei subsp . paracasei , Lactobacillus plantarum, Lactobacillus plantarum subsp . plantarum , Lactobacillus reuteri , Lactobacillus rhamnosus , Lactobacillus ruminis), Lactobacillus salivarius (Lactobacillus salivarius , Lactobacillus sp . ), Marinobacter ( Marinobacter < RTI ID = 0.0 > hydrocarbonoclasticus), Bacillus methyl Plastic gelatinase tooth (Methylobacillus flagellatus , Rhizobium grahamii , Rhizobium reguminosa , and so on. Rhizobium leguminosarum bv . Trifolii , Rhizobium < RTI ID = 0.0 > lupini , Rhizobium sp . ), Staphylococcus epidermidis (Staphylococcus epidermidis), Streptococcus Thermo filler's (Streptococcus thermophilus), Streptomyces Abbe US subtilis (Streptomyces avermitilis , and Streptomyces viridochromogenes . The present invention is not limited to these enzymes.

본 발명에서 사용한 프럭토스 1,6-비스포스페이트 알돌레이즈 효소의 유전자는 기존에 이미 유전자가 알려져 있지만 프럭토스-6-포스페이트를 이용한 4번 탄소 에피머화 작용 특성이 규명이 안 된 유전자 생산 균주들인 표 3에 기재된 균주들 유래의 프럭토스 1,6-비스포스페이트 알돌레이즈화 효소 유전자 또는 단백질에 대응되는 유전자를 클로닝하고, 상기 유전자를 포함한 발현벡터로 형질전환된 미생물을 배양하여 프럭토스 1,6-비스포스페이트 알돌레이즈 효소를 과발현시켜 효소의 특성을 규명한 결과, 기질특이성이 프럭토스-6-포스페이트 4번 탄소 에피머화 작용을 나타내는 효소임을 입증하고, 상기 효소를 사용하여 타가토스-6-포스페이트를 생산 후 상업용 피타아제를 처리하여 타가토스를 생산하는 것을 특징으로 한다.The gene of the fructose 1,6-bisphosphate aldolase enzyme used in the present invention is a gene which is known in the past but has not been characterized by the 4th carbon epimerization action using fructose-6-phosphate Cloning a gene corresponding to a fructose 1,6-bisphosphate aldolase gene or a protein derived from the strains described in 3 above and culturing the microorganism transformed with the expression vector containing the gene to obtain fructose 1,6- As a result of overexpression of the bisphosphate aldolase enzyme to characterize the enzyme, it was proved that the substrate specificity was an enzyme exhibiting the fructose-6-phosphate 4-carbon epimerization, and the tagatose-6-phosphate After production, commercial phytase is treated to produce tagatose.

효소의 특성을 규명하기 위하여, 본 발명은 종래 실험을 통해 전혀 기능적으로 규명되지 않고 다만 염기서열의 특성만으로 평가되어 명명된 공지의 프럭토스 1,6-비스포스페이트 알돌레이즈 효소의 유전자를 함유하고 있는 균주로부터 중합효소연쇄반응(polymerase chain reaction; PCR)을 통해 프럭토스 1,6-비스포스페이트 알돌레이즈 효소 코딩 유전자를 대량으로 수득한 후, 적절한 발현 벡터에 삽입하여 프럭토스 1,6-비스포스페이트 알돌레이즈 유전자를 포함하는 재조합 벡터를 제조한 후 상기 재조합 벡터로 적절한 미생물에 형질전환시킨 균주를 발효 배지에서 배양하여 과발현시킨 효소를 정제하여 사용한다.In order to elucidate the characteristics of the enzyme, the present invention includes a gene encoding a known fructose 1,6-bisphosphate aldolase enzyme, which is not fully functionally identified but evaluated only by the characteristics of the base sequence through conventional experiments A large amount of the fructose 1,6-bisphosphate aldolase enzyme coding gene was obtained from the strain by polymerase chain reaction (PCR), and inserted into an appropriate expression vector to obtain fructose 1,6-bisphosphate aldol A recombinant vector containing the raises gene is prepared, and a strain transformed into the appropriate microorganism as the recombinant vector is cultured in a fermentation medium to purify an over-expressed enzyme.

본 발명에 따라 수득한 프럭토스 1,6-비스포스페이트 알돌레이즈 효소는 금속 이온에 의해 활성화가 조절되는 금속효소(metalloenzyme)이다.The fructose 1,6-bisphosphate aldolase enzyme obtained according to the present invention is a metalloenzyme which is activated by metal ions.

상기 프럭토스 1,6-비스포스페이트 알돌레이즈 효소와 프럭토스와의 반응 조건으로 기질 농도는 55-75%(w/v)의 범위로 pH 8.5에서 50℃로 반응시키는 것이 타가토스 생산수율에 있어 바람직하다. 이는 프럭토스의 기질 농도가 55-75%(w/v) 범위에서 타가토스 생산수율이 우수하였으며, 프럭토스 1,6-비스포스페이트 알돌레이즈화 효소의 최적 pH와 온도 범위이기 때문이다.In the reaction conditions of the fructose 1,6-bisphosphate aldolase enzyme and fructose, the reaction at a substrate concentration of 55-75% (w / v) at a pH of 8.5 to 50 ° C resulted in a yield of tagatose desirable. This is because the yield of tagatose production was excellent in the range of substrate concentration of fructose in the range of 55-75% (w / v) and the optimal pH and temperature range of fructose 1,6-bisphosphate aldolase.

상기 프럭토스 1,6-비스포스페이트 알돌레이즈 효소는 서열번호 1 내지 72의 아미노산 서열로 이루어진 군으로부터 선택되는 어느 하나의 아미노산 서열을 가질 수 있으나, 이에 제한되지 않는다.The fructose 1,6-bisphosphate aldolase enzyme may have any amino acid sequence selected from the group consisting of the amino acid sequences of SEQ ID NOS: 1 to 72, but is not limited thereto.

본 발명의 일 구현예에 따른 조성물에서, 상기 프럭토카이네이즈는 바실러스 서브틸리스(Bacillus subtilis), 에세리키아 콜라이(Escherichia coli), 에세리키아 콜라이 K12 균주(Escherichia coli strain K12), 푸소박테리움 모르티페룸(Fusobacterium mortiferum), 클렙시엘라 뉴모니애(Klebsiella pneumoniae), 락토코커스 락티스 아종 크레모리스(Lactococcus lactis subsp . cremoris (Streptococcus cremoris)), 락토코커스 락티스 아종 락티스(Lactococcus lactis subsp . lactis (Streptococcus lactis)), 페디오코커스 펜토사세우스(Pediococcus pentosaceus), 리조비움 레규미노사룸 비브이. 트리폴리아이(Rhizobium leguminosarum bv . trifolii), 살모넬라 톰슨(Salmonella thompson), 살모넬라 티피무리움(Salmonella typhimurium), 스트렙토코커스 뮤탄스 세로타입 c 균주(Streptococcus mutans serotype c strain), 비브리오 알지노리티쿠스(Vibrio alginolyticus) 및 자이모모나스 모빌리스 아종 모빌리스 균주(Zymomonas mobilis subsp . mobilis strain)로 이루어진 군으로부터 선택되는 미생물로부터 유래한 효소일 수 있으나, 이에 제한되지 않는다. 바람직하게는 상기 프럭토카이네이즈는 서열번호 73 내지 86의 아미노산 서열로 이루어진 군으로부터 선택되는 어느 하나의 아미노산 서열을 갖는 효소일 수 있으나, 이에 제한되지 않는다.In a composition according to an embodiment of the invention, the fructokinase is selected from the group consisting of Bacillus subtilisBacillus subtilis), Escherichia coli (Escherichia coli), Escherichia coli K12 strain (Escherichia coli strain K12), fusobacterium moriferium (Fusobacterium mortiferum), Klebsiella pneumoniae (Klebsiella pneumoniae), Lactococcus lactis subsp. Cremoris (Lactococcus lactis subsp . cremoris (Streptococcus cremoris)), Lactococcus lactis subunit lactis (Lactococcus lactis subsp . lactis (Streptococcus lactis), Pediococcus pentosaceus (Pediococcus pentosaceus), Riboidum Resinumarosa Vivi. Tripoli Eye (Rhizobium leguminosarum bv . trifoli), Salmonella Thompson (Salmonella thompson), Salmonella typhimurium (Salmonella typhimurium), Streptococcus mutans Strain type c strain (Streptococcus mutans serotype c strain), Vibrio alginolyticus (Vibrio alginolyticus) And Zymomonas mobilis subsp. Mobilis strain (Zymomonas mobilis subsp .but are not limited to, enzymes derived from microorganisms selected from the group consisting of: Preferably, the fructokinase is an enzyme having an amino acid sequence selected from the group consisting of the amino acid sequences of SEQ ID NOs: 73 to 86, but is not limited thereto.

본 발명의 일 구현예에 따른 조성물에서, 상기 헥소카이네이즈는 캔디다 알비칸스(Candida albicans), 에메리셀라 니둘란스(Emericella nidulans), 클루이베로마이세스 락티스(Kluyveromyces lactis), 네오사르토리아 푸미가타(Neosartorya fumigata), 사카로마이세스 세레비지애(Saccharomyces cerevisiae), 스키조사카로마이세스 폼베(Schizosaccharomyces pombe) 및 쉬바니오마이세스 옥시덴탈리스(Schwanniomyces occidentalis)로 이루어진 군으로부터 선택되는 미생물로부터 유래한 효소일 수 있으나, 이에 제한되지 않는다. 바람직하게는 상기 헥소카이네이즈는 서열번호 87 내지 93의 아미노산 서열로 이루어진 군으로부터 선택되는 어느 하나의 아미노산 서열을 갖는 효소일 수 있으나, 이에 제한되지 않는다.In the composition according to an embodiment, the kinase is hekso Candida albicans (Candida albicans , Emericella nidulans), Cluj Vero Mai Seth lactis (Kluyveromyces lactis , Neosartorya < RTI ID = 0.0 > fumigata , Saccharomyces cerevisiae , Schizosaccharomyces, pombe ) and Schwanniomyces oxydentalis ( Schwanniomyces but are not limited to, enzymes derived from microorganisms selected from the group consisting of P. occidentalis . Preferably, the hexokinase is an enzyme having any one of the amino acid sequences selected from the group consisting of the amino acid sequences of SEQ ID NOs: 87 to 93, but is not limited thereto.

본 발명의 일 구현예에 따른 조성물에서, 상기 헥소카이네이즈 또는 프럭토카이네이즈; 프럭토스 1,6-비스포스페이트 알돌레이즈; 및 타가토스-6-포스페이트 피타아제 효소들은 고정화된 효소일 수 있으며, 고정화 방법은 흡착법, 공유결합법 또는 가두기법(entrapment)일 수 있으나, 이에 제한되지 않는다.In a composition according to an embodiment of the present invention, said hexokinase or fructokinase; Fructose 1,6-bisphosphate aldolase; And tagatose-6-phosphate phytase enzymes may be immobilized enzymes, and the immobilization method may be an adsorption method, a covalent bonding method, or an entrapment method, but is not limited thereto.

본 발명에서 프럭토스로부터 타가토스를 생산하기 위한 효소인 프럭토카이네이즈, 프럭토스 1,6-비스포스페이트 알돌에이즈 및 피타아제 효소를 각각 고정화시키기 위한 담체로 알긴산나트륨을 이용할 수 있으나, 이에 제한되지 않는다.In the present invention, sodium alginate may be used as a carrier for immobilizing fructokinase, fructose 1,6-bisphosphate aldolase, and phytase enzyme, which are enzymes for producing tagatose from fructose, but not limited thereto .

산업적으로 대량생산을 위해서, 효소를 이용한 생물공정은 화학공정에 비해 온화한 반응조건에서 반응이 진행되어 화학공정에 비해 에너지가 절약되며, 공정이 단순하여 공정비용이 적게 드는 장점이 있다. 하지만 이용되는 효소의 가격이 고가이며, 효소의 낮은 안정성이 극복해야 할 단점이다. 이러한 문제를 해결하고 공정비용의 감소를 위해 효소의 고정화가 널리 이용되고 있다. 효소를 고정화하면 반응에 사용한 효소를 다음 반응에 재사용할 수 있어 효소의 높은 가격 문제를 해결하여 공정비용을 감소시킬 수 있으며, 고정화하지 않는 효소보다 안정성이 증대되어 공정에 더 효율적으로 이용할 수 있다.For industrial mass production, bioprocessing using enzymes is advantageous in that the reaction proceeds under mild reaction conditions as compared with chemical processes, which saves energy compared to chemical processes and simplifies the process and reduces the process cost. However, the price of the enzyme used is high, and the low stability of the enzyme is a disadvantage to overcome. To solve these problems and to reduce the process cost, the immobilization of enzymes is widely used. When the enzyme is immobilized, the enzyme used in the reaction can be reused in the next reaction, thereby solving the problem of the high price of the enzyme, thereby reducing the process cost and increasing the stability of the enzyme.

생산성을 향상시키기 위한 방법으로 세포를 고정화시키는 방법이 있는데, 세포를 고정화하는 이유는 일반적으로, 이차대사산물의 생합성이 유도(induction)되면, 그 생합성 속도는 빠르게 증가하여 최고값에 도달한 후, 점차 생합성 속도가 감소한다고 알려져 있다. 특히, 연속배양 또는 장기간의 회분식 배양시 흔히 관찰되는 현상으로서 돌연변이에 의해 얻은 고생산성 균주를 장기간 배양할 경우에 균주의 복귀돌연변이(back mutation)가 발생한다. 복귀 돌연변이 발생에 의해 생산성이 낮은 세포는 원래 균주보다 생장속도가 빨라, 결국 이들 세포가 배양기를 차지함으로써 공정의 생산성을 떨어뜨린다. 이와 비슷한 경우가 재조합 단백질을 생산하는 공정에서도 많이 지적되고 있다. 이와 같은 공정 안정성(operational stability)에 대한 문제는 이차대사산물 생산공정 개발에 있어서 필수적으로 해결해야 할 문제들 중의 하나이다. 이를 해결할 수 있는 방법들 중의 하나가 고정화세포를 이용한 공정으로서, 세포를 고정화할 경우 유전적 안정성(genetic stability)이 증가한다고 알려져 있다.As a method for improving productivity, there is a method of immobilizing cells. The reason for immobilizing cells is generally that, when biosynthesis of secondary metabolites is induced, the biosynthesis rate rapidly increases to reach a maximum value, It is known that the rate of biosynthesis gradually decreases. In particular, when a highly productive strain obtained by mutation is cultured for a long time as a phenomenon frequently observed in continuous culture or in a batch culture for a long period of time, a back mutation of the strain occurs. Cells with low productivity due to the return mutation cause the growth rate of the cells to be higher than that of the original strain, and as a result, these cells take up the incubator and decrease the productivity of the process. Similar cases have been pointed out in the process of producing recombinant proteins. Such a problem of operational stability is one of the problems that must be solved in the development of secondary metabolite production process. It is known that one of the ways to solve this problem is the process using immobilized cells, in which the genetic stability is increased when cells are immobilized.

효소 또는 세포를 고정화시켜 생물반응제품을 생산하는 방법은 제조원가의 절감 등 그 경제적인 유용성으로 인해 지금까지 많은 연구가 행하여져 왔으며, 글루코스 이소머라제(Glucose isomerase) 고정화에 의한 프럭토스의 생산, 아미노아실라제(Aminoacylase) 고정화에 의한 아미노산의 생산 등이 공업적으로 실제 이용되고 있다.A number of studies have been conducted to date on methods for producing biologically active products by immobilizing enzymes or cells due to their economical advantages such as reduction of manufacturing costs and the production of fructose by the immobilization of glucose isomerase, And production of amino acids by immobilization of L-amino acid (L-amino acid) is practically used industrially.

일반적인 고정화 방법으로는 흡착법, 공유결합법, 포괄법(가두기법) 등이 있는데, 흡착법은 담체에 효소 또는 세포가 물리적으로 고정화되므로 연속운전으로 공업적 응용시 탈착이 우려되고, 공유결합법은 결합력은 강하나 효소의 실활이 쉽게 일어나기 때문에 실제 공업적으로는 알지네이트 등을 사용한 포괄법이 많이 이용되고 있다. 포괄법으로 효소나 세포를 고정화시켜 공업적으로 이용하고자 할 때는 먼저 담체의 선택이 중요한데, 가격이 저렴하고, 고정화 후 별도의 강도처리공정이 필요 없으며, 장기간 연속운전에도 안정한 제반 특성을 갖춘 것이 바람직하다.The adsorption method physically immobilizes enzymes or cells on a carrier, and desorption is likely to occur during industrial application due to continuous operation. The covalent bonding method is a method in which binding (binding) Since the enzyme is strong and the enzyme is inactivated easily, a comprehensive method using alginate or the like is widely used industrially. When an enzyme or a cell is immobilized by the inclusion method, it is preferable to select the carrier first, because it is low in price, does not require a separate strength treatment process after immobilization, and has various characteristics stable for long-term continuous operation Do.

또한, 본 발명은 프럭토스(fructose)에 헥소카이네이즈 또는 프럭토카이네이즈; 프럭토스 1,6-비스포스페이트 알돌레이즈; 및 타가토스-6-포스페이트 피타아제;를 첨가하여 반응시키는 단계를 포함하는 프럭토스(fructose)에서 타가토스(tagatose)를 생산하는 방법을 제공한다.The present invention also relates to a pharmaceutical composition comprising fructose, hexokinase or fructokinase; Fructose 1,6-bisphosphate aldolase; And adding tagatose-6-phosphate phytase to the fructose to produce a tagatose in fructose.

본 발명의 일 구현예에 따른 방법에서, 상기 효소들은 고정화된 효소일 수 있으나, 이에 제한되지 않는다.In the method according to one embodiment of the present invention, the enzymes may be immobilized enzymes, but are not limited thereto.

본 발명의 일 구현예에 따른 방법에서, 헥소카이네이즈 또는 프럭토카이네이즈; 프럭토스 1,6-비스포스페이트 알돌레이즈; 및 타가토스-6-포스페이트 피타아제 효소는 전술한 바와 같다.In a method according to one embodiment of the invention, hexokinase or fructokinase; Fructose 1,6-bisphosphate aldolase; And the tagatose-6-phosphate phytase enzyme are as described above.

본 발명의 혼합효소 반응은 다른 한편으로는 프럭토카이네이즈, 프럭토스 1,6-비스포스페이트 알돌레이즈화 효소, 피타아제를 프럭토스와 함께 세포 내에서 반응시켜 타가토스를 대량으로 생산하기 위한 방법으로, 대량으로 발현된 카이네이즈 효소, 프럭토스 1,6-비스포스페이트 알돌레이즈 효소를 과발현시켜 세포 내에서 사용하는 게 바람직하다. 이는 세포 내에 과발현된 효소는 활성이 장기간 유지될 수 있는 환경을 조성해 주고, 반응 시 필요한 cofactor 재생 가능성 때문인데, 본 발명에서 사용 가능한 대장균은 효소들을 과발현할 수 있는 대장균이라면 그 어느 것을 사용해도 무방하다.On the other hand, the mixed enzyme reaction of the present invention is a method for mass production of tagatose by reacting fructokinase, fructose 1,6-bisphosphate aldolase, and phytase together with fructose in a cell , A large amount of expressed kinase enzyme, and fructose 1,6-bisphosphate aldolase enzyme are preferably overexpressed and used in cells. This is because the enzyme overexpressed in the cell can provide an environment in which the activity can be maintained for a long period of time and is required for regeneration of the cofactor required for the reaction. Any of the Escherichia coli that can overexpress the enzymes usable in the present invention can be used .

본 발명에 따른 타가토스 생산 방법은 미생물로부터 얻은 효소를 이용하므로 환경 친화적이며, 간단한 효소 고정화 과정만을 필요로 하고, 프럭토스로부터 타가토생산을 이전에 없던 방법으로 전환시키고, 생산경비를 크게 줄이는 한편 생산 효과를 극대화할 수 있다.Since the method of producing tagatose according to the present invention uses an enzyme obtained from a microorganism, it is environmentally friendly, requires only a simple enzyme immobilization process, converts tagatose production from a fructose to a previously unneeded method, It is possible to maximize the production effect.

또한, 본 발명은 상기 방법에 의하여 생산된 타가토스를 유효성분으로 포함하는 식품 조성물을 제공한다.The present invention also provides a food composition comprising the tagatose produced by the above method as an active ingredient.

상기 식품의 종류에는 특별한 제한은 없다. 상기 타가토스를 첨가할 수 있는 식품의 예로는 장류, 육류, 소세지, 빵, 쵸코렛, 캔디류, 스낵류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림류를 포함한 낙농제품, 각종 스프, 음료수, 차, 드링크제, 알코올 음료 및 비타민 복합제 등이 있으며, 통상적인 의미에서의 식품을 모두 포함한다.
There is no particular limitation on the kind of the food. Examples of the foods to which the tagatose can be added include dairy products including soups, meats, sausages, breads, chocolates, candies, snacks, confectionery, pizza, ramen noodles, other noodles, gums, ice cream, , A drink, an alcoholic beverage, and a vitamin complex, and includes foods in a conventional sense.

이하, 본 발명의 내용을 하기 실시예를 통해 더욱 상세히 설명하고자 한다. 다만, 본 발명의 권리범위가 하기 실시예에만 한정되는 것은 아니고, 그와 등가의 기술적 사상의 변형까지를 포함한다.
Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following embodiments, and includes modifications of equivalent technical ideas.

실시예Example 1.  One. 프럭토카이네이즈Fructo canine 효소 생산 Enzyme production

프럭토카이네이즈 효소 유전자는 바실러스 서브틸리스(Bacillus subtilis str. 168)의 DNA 염기서열을 기본으로 프라이머(primer)를 도안하여 PCR 증폭하여 대량으로 얻은 다음, 제한효소 XhoI와 NdeI을 사용하여 pET Duet-1[Novagen]에 삽입하고, 재조합 벡터 프럭토카이네이즈/pET Duet-1를 제조하여, 이를 통상적인 형질전환 방법에 의해 대장균 BL21(DE3)에 형질전환하였다. 대량생산을 위한 배양을 하기 전에 액체질소에서 냉동보관 하였다. 그런 다음, 프럭토카이네이즈 효소를 대량생산하기 위하여, 먼저, 냉동 보관된 BL(DE21) 균주를 LB 배지 50ml가 들어있는 250ml의 플라스크에 접종하여, 600nm에서의 흡광도가 2.0이 될 때까지 37℃의 진탕배양기에서 종균배양을 하고, 상기 종균배양된 배양액을 발효 배지(글리세롤 10 g/l, 펩톤 1g/l, 효모 추출물 30 g/l, 이인산칼륨 0.14 g/l, 일인산나트륨 1 g/l) 5l가 들어있는 7l의 발효조(바이오트론, 한국)에 첨가하여 본 배양을 수행하였다. 이때, 600nm에서의 흡광도가 2.0이 될 때, 1mM ITPG를 첨가하여 프럭토카이네이즈 효소의 대량생산을 유도하였다. 이때, 상기 과정 중의 교반 속도는 500 rpm, 통기량은 1.0vvm, 배양 온도는 37℃가 유지되도록 하였다.The fructokinase enzyme gene was amplified by PCR using a primer designed on the basis of the DNA sequence of Bacillus subtilis str. 168, and then the plasmid was digested with restriction enzymes XhoI and NdeI to obtain pET Duet- 1 [Novagen] to prepare a recombinant vector fructokaine / pET Duet-1, which was transformed into E. coli BL21 (DE3) by a conventional transformation method. And stored frozen in liquid nitrogen prior to culture for mass production. Then, in order to mass-produce the fructokinase enzyme, firstly, a frozen BL (DE21) strain was inoculated into a 250 ml flask containing 50 ml of LB medium and incubated at 37 ° C until the absorbance at 600 nm reached 2.0 The cultured medium was cultured in a fermentation medium (glycerol 10 g / l, peptone 1 g / l, yeast extract 30 g / l, diphosphate 0.14 g / l, sodium monophosphate 1 g / l ) Was added to a 7 liter fermenter (Biotron, Korea) containing 5 liters, and the present cultivation was carried out. At this time, when the absorbance at 600 nm was 2.0, 1 mM ITPG was added to induce mass production of fructokinase enzyme. At this time, the stirring speed was 500 rpm, the aeration amount was 1.0 vvm, and the incubation temperature was maintained at 37 캜.

프럭토카이네이즈 효소의 정제를 위해서 DEAE-Sephacel, Blue-Sepharose CL-6B, 5'-AMP-Sepharose 4B 컬럼을 사용하여 정제하였다.For purification of fructokinase enzyme, it was purified using DEAE-Sephacel, Blue-Sepharose CL-6B, 5'-AMP-Sepharose 4B column.

정제한 효소의 분자량을 측정한 결과 32,440 Da 단량체 두 개로 구성된 다이머(이량체)이고, 이의 아미노산 서열은 NCBI 등록번호(accession number) BAA19710와 동일함을 확인할 수 있었다(도 2, 레인 2).
The molecular weight of the purified enzyme was measured and it was confirmed that the amino acid sequence thereof was the same as that of NCBI accession number BAA19710 (Fig. 2, lane 2).

실시예Example 2.  2. 헥소카이네이즈Hexo canine 효소 생산 Enzyme production

헥소카이네이즈 효소 유전자는 클루이베로마이세스 락티스(Kluyveromyces lactis ATCC8585)의 DNA 염기서열을 기본으로 프라이머(primer)를 도안하여 PCR 증폭하여 대량으로 얻은 다음, 제한효소 XhoI와 NdeI을 사용하여 pET Duet-1[Novagen]에 삽입하고, 재조합 벡터 헥소카이네이즈/pET Duet-1를 제조하여, 이를 통상적인 형질전환 방법에 의해 대장균 BL21(DE3)에 형질전환하였다. 대량생산을 위한 배양을 하기 전에 액체질소에서 냉동보관 하였다. 그런 다음, 헥소카이네이즈 효소를 대량생산하기 위하여, 먼저, 냉동 보관된 BL(DE21) 균주를 LB 배지 50ml가 들어있는 250ml의 플라스크에 접종하여, 600nm에서의 흡광도가 2.0이 될 때까지 37℃의 진탕배양기에서 종균배양을 하고, 상기 종균배양된 배양액을 발효 배지(글리세롤 10 g/l, 펩톤 1g/l, 효모 추출물 30 g/l, 이인산칼륨 0.14 g/l, 일인산나트륨 1 g/l) 5l가 들어있는 7l의 발효조(바이오트론, 한국)에 첨가하여 본 배양을 수행하였다. 이때, 600nm에서의 흡광도가 2.0이 될 때, 1mM ITPG를 첨가하여 헥소카이네이즈 효소의 대량생산을 유도하였다. 이때, 상기 과정 중의 교반 속도는 500 rpm, 통기량은 1.0vvm, 배양 온도는 37℃가 유지되도록 하였다.The hexokinase enzyme gene was amplified by PCR using a primer designed on the basis of the nucleotide sequence of Kluyveromyces lactis ATCC8585. The hexo-kinase enzyme gene was digested with restriction enzymes XhoI and NdeI to obtain pET Duet-1 [Novagen] to prepare a recombinant vector hexokaine / pET Duet-1, which was transformed into Escherichia coli BL21 (DE3) by a conventional transformation method. And stored frozen in liquid nitrogen prior to culture for mass production. Then, in order to mass-produce the hexokinase enzyme, the frozen BL (DE21) strain was first inoculated into a 250 ml flask containing 50 ml of LB medium and shaken at 37 ° C until the absorbance at 600 nm reached 2.0. (10 g / l of glycerol, 1 g / l of peptone, 30 g / l of yeast extract, 0.14 g / l of dibasic potassium phosphate and 1 g / l of sodium monophosphate) were added to the fermentation medium Was added to a 7 liter fermenter (Biotron, Korea) containing 5 liters and the cultivation was performed. At this time, when the absorbance at 600 nm was 2.0, 1 mM ITPG was added to induce mass production of hexokinase enzyme. At this time, the stirring speed was 500 rpm, the aeration amount was 1.0 vvm, and the incubation temperature was maintained at 37 캜.

헥소카이네이즈 효소를 정제하기 위해 DEAE-셀룰로즈, 포스포셀룰로즈(phosphocellulose)와 Sephadex G-200을 사용하였다.DEAE-cellulose, phosphocellulose and Sephadex G-200 were used to purify the hexokinase enzyme.

정제한 효소의 분자량을 측정한 결과 53,610 Da의 단량체 두 개로 구성된 다이머(이량체)임을 알 수 있었으며, 이의 아미노산 서열은 NCBI 등록번호(accession number) CAA43855와 동일함을 확인할 수 있었다(도 2, 레인 1).
The molecular weight of the purified enzyme was found to be a dimer composed of two monomers of 53,610 Da, and its amino acid sequence was confirmed to be the same as NCBI accession number CAA43855 (Fig. 2, lane One).

실시예Example 3. 프럭토카이네이즈 및  3. Fructokinase and 헥소카이네이즈에Hexo canine 의한 프럭토스로부터 프럭토스 6- Lt; RTI ID = 0.0 > 6- 포스페이트로의Phosphate 전환 활성분석 Conversion activity assay

본 발명의 고농도의 타가토스의 생산을 위하여 첫 단계로 5, 10, 25, 50 mM 프럭토스를 동량의 ATP (Adenosine triphosphate)와 사카로마이세스 세레비지애(Saccharomyces cerevisea) 유래의 헥소카이네이즈(sigma-aldrich에서 구매)를 반응하여 프럭토스 6-포스페이트를 생산하기 위하여, 250 unit/ml의 효소가 함유된 50 mM 트리즈마 베이스 완충용액에서 pH 7.5, 온도 30℃에서 60분간 반응시켜서 생성된 프럭토스 6-포스페이트의 양으로 효소의 활성을 측정하였다. 효소 농도에 따른 프럭토스 6-포스페이트 생산량은 도 3에 나타내었다. 이의 결과, 5, 10, 25, 50 mM 모두의 농도에서 전환 수율 90% 정도로 생산되었다.
For the production of the high concentration of tagatose of the present invention, 5, 10, 25, and 50 mM fructose were added to the same amount of ATP (Adenosine triphosphate) and Saccharomyces In order to produce fructose 6-phosphate by reacting with hexose canease (purchased from Sigma-Aldrich) originating from S. cerevisea , 50 mM Tris-base buffer solution containing 250 units / ml of enzyme was added at pH 7.5, The reaction was carried out for 60 minutes, and the activity of the enzyme was measured by the amount of fructose 6-phosphate produced. The yield of fructose 6-phosphate according to enzyme concentration is shown in Fig. As a result, a conversion yield of about 90% was obtained at the concentrations of 5, 10, 25 and 50 mM.

실시예Example 4. 균주별 프럭토카이네이즈 4. Fructokinase by strain 의of 프럭토스에서 프럭토스 6-포스페이트로의 전환 활성 비교 Comparison of conversion activity from fructose to fructose 6-phosphate

다양한 균주 유래의 프럭토카이네이즈를 이용하여 상기 실시예 3에 기재된 방법과 같이 프럭토카이네이즈의 프럭토스에서 프럭토스 6-포스페이트로의 전환 활성을 비교한 결과는 표 1과 같다.Table 1 shows the results of comparing the fructose 6-phosphate conversion activity of fructokinase from the fructokinase-derived fructokinase as described in Example 3. The results are shown in Table 1. < tb > < TABLE >

속명Genius 균주명Strain name 서열번호SEQ ID NO: 활성(%)activation(%) Bacillus Bacillus Bacillus subtilisBacillus subtilis 7373 9292 EscherichiaEscherichia Escherichia coliEscherichia coli 7474 9393 EscherichiaEscherichia Escherichia coli strain K-12 Escherichia coli strain K-12 7575 9393 FusobacteriumFusobacterium Fusobacterium mortiferumFusobacterium mortiferum 7676 9090 Klebsiella Klebsiella KlebsiellaKlebsiella pneumoniapneumonia 7777 9292 Lactococcus Lactococcus Lactococcus lactisLactococcus lactis 78,7978,79 9191 Pediococcus Pediococcus Pediococcus pentosaceusPediococcus pentosaceus 8080 9090 Rhizobium Rhizobium Rhizobium leguminosarum Rhizobium leguminosarum 8181 9191 Salmonella Salmonella Salmonella thompsonSalmonella thompson 8282 9090 Salmonella typhimuriumSalmonella typhimurium 8383 9191 StreptococcusStreptococcus Streptococcus mutans Streptococcus mutans 8484 9292 VibrioVibrio Vibrio alginolyticusVibrio alginolyticus 8585 9292 ZymomonasZymomonas Zymomonas mobilis Zymomonas mobilis 8686 9393

실시예Example 5. 균주별  5. By strain 헥소카이네이즈의Hexo canine 프럭토스에서 프럭토스 6-포스페이트로의 전환 활성 비교 Comparison of conversion activity from fructose to fructose 6-phosphate

다양한 균주 유래의 헥소카이네이즈를 이용하여 상기 실시예 3에 기재된 방법과 같이 헥소카이네이즈의 프럭토스에서 프럭토스 6-포스페이트로의 전환 활성을 비교한 결과는 표 2와 같다.Table 2 shows the results of comparing the conversion activity of hexokinase from fructose to fructose 6-phosphate using the hexokinase derived from various strains, as in the method described in Example 3 above.

속명Genius 균주명Strain name 서열번호SEQ ID NO: 활성(%)activation(%) CandidaCandida Candida albicansCandida albicans 8787 9191 EmericellaEmericella Emericella nidulansEmericella nidulans 8888 9393 KluyveromycesKluyveromyces Kluyveromyces lactisKluyveromyces lactis 8989 9292 NeosartoryaNeosartoria Neosartorya fumigataNeosarty fumigata 9090 9090 SaccharomycesSaccharomyces Saccharomyces cerevisiaeSaccharomyces cerevisiae 9191 9191 SchizosaccharomycesSchizosaccharomyces Schizosaccharomyces pombeSchizosaccharomyces pombe 9292 9090 SchwanniomycesSchwanniomyces Schwanniomyces occidentalisSchwanniomyces occidentalis 9393 8989

실시예Example 6.  6. 프럭토스Fructose 1,6- 1,6- 비스포스페이트Bisphosphate 알돌레이즈Aldol Reyes 효소의 대량생산 Mass production of enzymes

프럭토스 1,6-비스포스페이트 알돌레이즈 효소 유전자는 바실러스 서브틸리스 아종 인아쿠오소룸(Bacillus subtilis subsp. inaquosorum) KCTC 13429, 코리네박테리움 글루타미쿰( Corynebacterium glutamicum), 스트렙토코커스 써모필루스(Streptococcus thermophilus)의 DNA 염기서열을 기본으로 프라이머(primer)를 도안하여 PCR 증폭하여 대량으로 얻은 다음, 제한효소 XhoI와 NdeI을 사용하여 pET Duet-1[Novagen]에 삽입하고, 재조합 벡터 pET Duet-1/프럭토스 1,6-비스포스페이트 알돌레이즈(도 6 참조)를 제조하여, 이를 통상적인 형질전환 방법에 의해 대장균 BL21(DE3)에 형질전환하였다. 그리고, 대량생산을 위한 배양을 하기 전에 액체질소에서 냉동보관 하였다. 그런 다음, 프럭토스 1,6-비스포스페이트 알돌레이즈 효소를 대량생산하기 위하여, 먼저, 냉동 보관된 BL(DE21) 균주를 LB 배지 50ml가 들어있는 250ml의 플라스크에 접종하여, 600nm에서의 흡광도가 2.0이 될 때까지 37℃의 진탕배양기에서 종균배양을 하고, 상기 종균배양된 배양액을 발효 배지(글리세롤 10 g/l, 펩톤 1g/l, 효모 추출물 30 g/l, 이인산칼륨 0.14 g/l, 일인산나트륨 1 g/l) 5l가 들어있는 7l의 발효조(바이오트론, 한국)에 첨가하여 본 배양하였다. 이때, 600nm에서의 흡광도가 2.0이 될 때, 1mM ITPG를 첨가하여 프럭토스 1,6-비스포스페이트 알돌레이즈 효소의 대량생산을 유도하였다. 이때, 상기 과정 중의 교반 속도는 500 rpm, 통기량은 1.0vvm, 배양 온도는 37℃가 유지되도록 하였다.
Fructose 1,6-bisphosphate aldolase gene is Bacillus subtilis subspecies inah Kuo sorum (Bacillus subtilis subsp. inaquosorum) KCTC 13429, Corynebacterium glutamicum (Corynebacterium glutamicum and Streptococcus thermophilus primers based on the nucleotide sequence of the Streptococcus thermophilus were amplified by PCR using a restriction enzyme XhoI and NdeI to obtain pET Duet-1 [Novagen] , And a recombinant vector pET Duet-1 / fructose 1,6-bisphosphate aldolase (see FIG. 6) was prepared and transformed into E. coli BL21 (DE3) by a conventional transformation method. And, they were frozen in liquid nitrogen before culturing for mass production. Then, in order to mass-produce the fructose 1,6-bisphosphate aldolase enzyme, the frozen BL (DE21) strain was first inoculated into a 250 ml flask containing 50 ml of LB medium to obtain an absorbance at 600 nm of 2.0 (10 g / l of glycerol, 1 g / l of peptone, 30 g / l of yeast extract, 0.14 g / l of dibasic potassium phosphate, 10 g / l of yeast extract, (1 g / l of sodium phosphate monobasic) was added to a 7 l fermenter (Biotron, Korea). At this time, when the absorbance at 600 nm was 2.0, 1 mM ITPG was added to induce mass production of fructose 1,6-bisphosphate aldolase enzyme. At this time, the stirring speed was 500 rpm, the aeration amount was 1.0 vvm, and the incubation temperature was maintained at 37 캜.

실시예Example 7.  7. 프럭토스Fructose 1,6- 1,6- 비스포스페이트Bisphosphate 알돌레이즈Aldol Reyes 효소의 정제 Purification of enzymes

프럭토스 1,6-비스포스페이트 알돌레이즈 효소의 특성을 정확히 파악하기 위해서 어피니티 히스트랩 에이치피(affinity HisTrap HP) 컬럼, 탈염 하이프렙(HiPrep 16/60), 겔여과 세파크릴 에스-100 에치알 (gel filtration Sephacryl S-100 HR) 컬럼을 사용하여 정제하였다.In order to accurately characterize the fructose 1,6-bisphosphate aldolase enzyme, an affinity HisTrap HP column, a desalting hyperep (HiPrep 16/60), a gel filtration sephacrylase-100 Echior ( gel filtration Sephacryl S-100 HR column.

정제한 효소의 분자량을 측정한 결과 바실러스 서브틸리스 아종 인아쿠오소룸(Bacillus subtilis subsp. inaquosorum) KCTC 13429의 효소는 31,408 Da (도 2, 레인 3), 코리네박테리움 글루타미쿰(Corynebacterium glutamicum)의 효소는 37,214 Da (도 2, 레인 4), 스트렙토코커스 써모필루스(Streptococcus thermophilus)의 효소는 31,555 Da (도 2, 레인 5) 각각의 단량체 두 개로 구성된 이량체임을 알 수 있었으며, 이의 아미노산 서열은 NCBI 등록번호(accession number) 각각 ELS63303, CAA35190, AAV61498와 동일함을 확인할 수 있었다.
The enzyme activity of purified Bacillus subtilis subsp. Inaquosorum KCTC 13429 was found to be 31,408 Da (Fig. 2, lane 3), Corynebacterium glutamicum , The enzyme of 37,214 Da (Fig. 2, lane 4) and the enzyme of Streptococcus thermophilus was found to be a dimer composed of two monomers of 31,555 Da (Fig. 2, lane 5). The amino acid sequence Were identical to the NCBI accession numbers ELS63303, CAA35190 and AAV61498, respectively.

실시예Example 8.  8. 프럭토스Fructose 1,6- 1,6- 비스포스페이트Bisphosphate 알돌레이즈Aldol Reyes 효소의 금속 특이성 The metal specificity of the enzyme

이전 보고서에서는 프럭토스 1,6-비스포스페이트 알돌레이즈 효소는 금속 아연에 의해서 프럭토스 1,6-비스포스페이트 기질이 디히드록시 아세트포스페이트와 글리세르 알데히드 3-포스페이트로 전환 시 관여되며 역가를 향상시킨다고 알려져 있으나, 본 발명에서는 기질을 프럭토스 6-포스페이트로 적용하였을 때 금속염 효과가 역가 향상에 영향을 미치지 않음을 보였다. 금속염 효과를 조사하기 위해서 효소활성은 도 4a에 나타낸 바와 같이, EDTA를 처리하거나 또는 1 mM 금속이온 첨가 후 측정하였으며, 이때 반응은 1.0%의 프럭토스와 0.04 unit/ml의 효소가 함유된 50 mM 피페스 완충용액에서 pH 8.5와 50℃에서 30분간 수행하였고 HCl 0.2M을 첨가하여 반응을 멈추어 측정하였다.In previous reports, the fructose 1,6-bisphosphate aldolase enzyme was involved in the conversion of fructose 1,6-bisphosphate substrate to dihydroxyacetophosphate and glyceraldehyde 3-phosphate by metal zinc, However, the present invention shows that the metal salt effect does not affect the potency enhancement when the substrate is applied to fructose 6-phosphate. In order to investigate the metal salt effect, enzyme activity was measured after treatment with EDTA or addition of 1 mM metal ion as shown in FIG. 4A. The reaction was carried out in the presence of 1.0% fructose and 50 mM The reaction was stopped at pH 8.5 and 50 ° C in Pepper buffer solution for 30 min and the addition of 0.2 M HCl was stopped.

본 발명의 프럭토스 1,6-비스포스페이트 알돌레이즈 효소는 금속이온에 의한 효소활성의 변화가 없었고, 이전 논문과 달리 아연 이온은 활성을 크게 저해하는 금속이온임을 알 수 있었다.
In the fructose 1,6-bisphosphate aldolase enzyme of the present invention, there was no change in the activity of the enzyme by the metal ion, and it was found that the zinc ion is a metal ion which greatly inhibits the activity, unlike the prior art.

실시예Example 9.  9. 프럭토스Fructose 1,6- 1,6- 비스포스페이트Bisphosphate 알돌레이즈Aldol Reyes 효소의  Enzymatic pHpH 및 온도 변화에 따른 활성 And activity due to temperature change

본 실시예는 pH 및 온도 변화에 따른 프럭토스 1,6-비스포스페이트 알돌레이즈 효소의 활성을 확인하기 위해, 다양한 pH 및 온도에서 효소와 기질을 반응시키고, 효소 활성을 비교하고자 하였다. 이때, pH 효과를 조사하기 위해서 반응은 1.0%의 프럭토스와 0.04 unit/ml의 효소가 함유된 50 mM 트리즈마 베이스(Trizma base) 완충용액 pH 6.5에서부터 10까지의 범위에서 반응시켰다. 이때, 반응 온도는 37℃에서 30분간 반응을 시켰다. 그런 다음, 0.2 M HCl을 첨가하여 반응을 종료하고, 효소의 활성을 측정하여 그 결과를 도 4b에 나타내었다.In order to confirm the activity of fructose 1,6-bisphosphate aldolase enzyme according to pH and temperature changes, the present example was to compare enzyme activity with enzyme and substrate at various pH and temperature. At this time, in order to investigate the pH effect, the reaction was carried out in a range of pH 6.5 to 10 in a 50 mM Trizma base buffer containing 1.0% of fructose and 0.04 unit / ml of enzyme. At this time, the reaction temperature was 37 ° C for 30 minutes. Then, the reaction was terminated by adding 0.2 M HCl, and the activity of the enzyme was measured. The results are shown in FIG. 4B.

그리고, 온도 효과를 조사하기 위해서 반응은 온도 20℃에서 70℃까지 1.0%의 프럭토스 6-포스페이트와 0.04 unit/ml의 효소가 함유된 50 mM 트리즈마 베이스 완충용액을 pH 8.5에서 30분간 반응을 시켰으며, 이때 0.2 M HCl을 첨가하여 반응을 종료하고 효소의 활성을 측정하여 그 결과를 도 4c에 나타내었다.In order to investigate the temperature effect, the reaction was carried out at a temperature of 20 ° C to 70 ° C for 30 minutes at pH 8.5 in a 50 mM Trzuma base buffer solution containing 1.0% of fructose 6-phosphate and 0.04 unit / ml of enzyme The reaction was terminated by addition of 0.2 M HCl, and the activity of the enzyme was measured. The results are shown in FIG. 4C.

그 결과, 최적 pH와 온도는 각각 8.5와 50℃이었다. 위 결과를 토대로 최적의 온도와 pH에서 프럭토스 6-포스페이트에서 타가토스 6-포스페이트로의 전환을 시간별로 나타내어 전환율이 85% 까지 도달하는 것을 확인하였고 도 4d에 나타내었다. 그러나 상기 반응에 있어 특정 pH와 온도를 규정하지 아니하고 원하는 수율이나 반응환경에 따라 어떠한 범주에서의 반응도 적용한다.
As a result, optimum pH and temperature were 8.5 and 50 ℃, respectively. Based on the above results, it was confirmed that the conversion rate from fructose 6-phosphate to tagatose 6-phosphate was reached to 85% at the optimal temperature and pH and was shown in FIG. 4d. However, the reaction does not specify a specific pH and temperature in the reaction but applies the reaction in any category depending on the desired yield or reaction environment.

실시예Example 10. 균주별 프럭토스 1,6- 10. Fructose by Strain 1.6- 비스포스페이트Bisphosphate 알돌레이즈Aldol Reyes 활성(프럭토스 6- Active (fructose 6- 포스페이트에서In phosphate 타가토스Tagatos 6- 6- 포스페이트로의Phosphate 전환 효율) 비교 Conversion efficiency) comparison

다양한 균주별 프럭토스 1,6-비스포스페이트 알돌레이즈 활성을 상기 실시예 9에 기재된 방법에 따라 측정하여 비교한 결과는 하기 표 3과 같다.The fructose 1,6-bisphosphate aldolase activity of various strains was measured according to the method described in Example 9, and the results are shown in Table 3 below.

속명Genius 균주명Strain name 서열번호SEQ ID NO: 전환율(%)Conversion Rate (%) AlcanivoraxAlcanivorax Alcanivorax borkumensis SK2 Alcanivorax borkumensis SK2 1One 9292 BacillusBacillus Bacillus alcalophilus ATCC 27647 Bacillus alcalophilus ATCC 27647 22 9393 Bacillus amyloliquefaciens DSM 7 Bacillus amyloliquefaciens DSM 7 33 9393 Bacillus azotoformans LMG 9581 Bacillus azotoformans LMG 9581 44 9494 Bacillus cellulosilyticus DSM 2522 Bacillus cellulosilyticus DSM 2522 55 9292 Bacillus coagulans 36D1 Bacillus coagulans 36D1 66 9191 Bacillus cytotoxicus NVH 391-98 Bacillus cytotoxicus NVH 391-98 77 9494 Bacillus halodurans C-125 Bacillus halodurans C-125 88 9494 Bacillus licheniformis DSM 13 = ATCC 14580 Bacillus licheniformis DSM 13 = ATCC 14580 99 9393 Bacillus macauensis ZFHKF-1 Bacillus macauensis ZFHKF-1 1010 9494 Bacillus megaterium DSM 319 Bacillus megaterium DSM 319 1111 9393 Bacillus methanolicus MGA3 Bacillus methanolicus MGA3 1212 9494 Bacillus nealsonii AAU1 Bacillus nealsonii AAU1 1313 9494 Bacillus pseudofirmus OF4 Bacillus pseudofirmus OF4 1414 9393 Bacillus selenitireducens MLS10 Bacillus selenitireducens MLS10 1515 9292 Bacillus sp. B14905 Bacillus sp . B14905 1616 9393 Bacillus subtilis subsp . inaquosorum KCTC13429 Bacillus subtilis subsp . inaquosorum KCTC13429 1717 9595 Bacillus weihenstephanensis KBAB4 Bacillus weihenstephanensis KBAB4 1818 9494 BifidobacteriumBifidobacterium BifidobacteriumBifidobacterium brevebreve 1919 9393 CorynebacteriumCorynebacterium Corynebacterium glutamicum (strain ATCC 13032) Corynebacterium glutamicum (strain ATCC 13032) 2020 9292 Corynebacterium kroppenstedtii DSM 44385 Corynebacterium kroppenstedtii DSM 44385 2121 9393 EnterococcusEnterococcus Enterococcus asini ATCC 700915 Enterococcus asini ATCC 700915 2222 9393 Enterococcus caccae ATCC BAA-1240 Enterococcus caccae ATCC BAA-1240 2323 9494 Enterococcus casseliflavus ATCC 12755 Enterococcus casseliflavus ATCC 12755 2424 9494 Enterococcus faecalis ATCC 29200 Enterococcus faecalis ATCC 29200 2525 9494 Enterococcus faecium ATCC 8459 Enterococcus faecium ATCC 8459 2626 9595 Enterococcus flavescens ATCC 49996 Enterococcus flavescens ATCC 49996 2727 9494 Enterococcus gilvus ATCC BAA-350 Enterococcus gilvus ATCC BAA-350 2828 9393 Enterococcus haemoperoxidus ATCC BAA-382 Enterococcus haemoperoxidus ATCC BAA-382 2929 9494 Enterococcus italicus DSM 15952 Enterococcus italicus DSM 15952 3030 9292 Enterococcus moraviensis ATCC BAA-383 Enterococcus moraviensis ATCC BAA-383 3131 9191 Enterococcus mundtii ATCC 882 Enterococcus mundtii ATCC 882 3232 9393 Enterococcus pallens ATCC BAA-351 Enterococcus pallens ATCC BAA-351 3333 9393 Enterococcus phoeniculicola ATCC BAA-412 Enterococcus phoeniculicola ATCC BAA-412 3434 9494 Enterococcus raffinosus ATCC 49464 Enterococcus raffinosus ATCC 49464 3535 9292 Enterococcus saccharolyticus ATCC 43076 Enterococcus saccharolyticus ATCC 43076 3636 9393 Enterococcus sp . C1 Enterococcus sp . C1 3737 9090 Enterococcus villorum ATCC 700913 Enterococcus villorum ATCC 700913 3838 9191 EscherichiaEscherichia Escherichia coli ATCC 25922, ATCC 700928 Escherichia coli ATCC 25922, ATCC 700928 3939 9393 Escherichia coli str. K-12 substr. MG1655 Escherichia coli str. K-12 substr. MG1655 4040 9494 Escherichia sp. TW09231 Escherichia sp . TW09231 4141 9292 GeobacillusGeobacillus Geobacillus kaustophilus HTA426 Geobacillus kaustophilus HTA426 4242 9393 Geobacillus sp. C56-T3 Geobacillus sp . C56-T3 4343 9494 GeobacillusGeobacillus stearothermophilusstearothermophilus 4444 9393 Geobacillus thermodenitrificans NG80-2 Geobacillus thermodenitrificans NG80-2 4545 9494 LactobacillusLactobacillus
















Lactobacillus acidophilus ATCC 4796 Lactobacillus acidophilus ATCC 4796 4646 9393 Lactobacillus amylolyticus DSM 11664 Lactobacillus amylolyticus DSM 11664 4747 9393 Lactobacillus antri DSM 16041 Lactobacillus antri DSM 16041 4848 9292 Lactobacillus casei BD-II Lactobacillus casei BD-II 4949 9393 Lactobacillus crispatus 125-2-CHN Lactobacillus crispatus 125-2-CHN 5050 9494 Lactobacillus curvatus CRL 705 Lactobacillus curvatus CRL 705 5151 9393 Lactobacillus delbrueckii subsp . bulgaricus PB2003/044-T3-4 Lactobacillus delbrueckii subsp . bulgaricus PB2003 / 044-T3-4 5252 9494 Lactobacillus helveticus DSM 20075 Lactobacillus helveticus DSM 20075 5353 9494 Lactobacillus iners ATCC 55195 Lactobacillus iners ATCC 55195 5454 9393 Lactobacillus johnsonii ATCC 33200 Lactobacillus johnsonii ATCC 33200 5555 9292 LactobacillusLactobacillus murinusmurinus 5656 9494 Lactobacillus paracasei subsp . paracasei 8700:2 Lactobacillus paracasei subsp . paracasei 8700: 2 5757 9393 Lactobacillus plantarum subsp . plantarum ATCC 14917 Lactobacillus plantarum subsp . plantarum ATCC 14917 5858 9494 Lactobacillus reuteri 100-23 Lactobacillus reuteri 100-23 5959 9494 Lactobacillus rhamnosus ATCC 21052 Lactobacillus rhamnosus ATCC 21052 6060 9393 Lactobacillus ruminis ATCC 25644 Lactobacillus ruminis ATCC 25644 6161 9494 Lactobacillus salivarius ATCC 11741 Lactobacillus salivarius ATCC 11741 6262 9494 Lactobacillus sp. ASF360 Lactobacillus sp . ASF360 6363 9393 MarinobacterMarinobacter Marinobacter hydrocarbonoclasticus ATCC 49840 Marinobacter hydrocarbonoclasticus ATCC 49840 6464 9494 MethylobacillusMethylobacillus Methylobacillus flagellatus KT Methylobacillus flagellatus KT 6565 9292 RhizobiumRhizobium Rhizobium leguminosarum bv . trifolii WSM1325 Rhizobium leguminosarum bv . trifoli WSM1325 6666 9494 Rhizobium lupini HPC(L) Rhizobium lupini HPC (L) 6767 9292 Rhizobium sp . AP16 Rhizobium sp . AP16 6868 9393 StaphylococcusStaphylococcus Staphylococcus epidermidis 36-1 Staphylococcus epidermidis 36-1 6969 9494 StreptococcusStreptococcus Streptococcus thermophilus MN-ZLW-002 Streptococcus thermophilus MN-ZLW-002 7070 9595 StreptomycesStreptomyces Streptomyces avermitilis MA-4680 Streptomyces avermitilis MA-4680 7171 9595 Streptomyces viridochromogenes DSM 40736 Streptomyces viridochromogenes DSM 40736 7272 9494

실시예Example 11.  11. 피타아제에On phytase 의한  by 타가토스Tagatos 6- 6- 포스페이트로부터From phosphate 타가토스Tagatos 생산 production

본 발명의 고농도의 타가토스의 생산을 위하여 10 mM 프럭토스 6-포스페이트, 10에서 50 unit/ml의 효소가 함유된 50 mM 트리즈마 베이스 완충용액에서 pH 5.5, 온도 60℃에서 60분간 반응을 수행하였다. 그런 다음, 효소의 활성을 측정하였다. 효소 농도에 따른 타가토스 생산량은 도 5에 나타내었다. For the production of the tagatose of high concentration of the present invention, the reaction was carried out in a buffer solution of 50 mM Trzuma base containing 10 mM fructose 6-phosphate and 10 to 50 unit / ml of enzyme at pH 5.5 and 60 ° C for 60 minutes Respectively. Then, the activity of the enzyme was measured. The production of tagatose according to the enzyme concentration is shown in Fig.

효소 농도 50 U/ml에 9 mM의 타가토스가 생산되었고 이것은 전환 수율 90%에 해당되는 것이다.
9 mM of tagatose was produced at an enzyme concentration of 50 U / ml, which corresponds to a conversion yield of 90%.

실시예Example 12.  12. 프럭토카이네이즈Fructo canine , , 프럭토스Fructose 1,6- 1,6- 비스포스페이트Bisphosphate 알돌레이즈Aldol Reyes 및  And 피타아제의Phytase 컴비네이션Combination 반응을 이용한  Reaction 프럭토스로부터From Fructose 타가토스Tagatos 생산 production

앞선 실시예들을 통한 결과를 토대로 프럭토카이네이즈, 알돌레이즈, 피타아제의 칵테일 반응을 이용한 프럭토스로부터 타가토스 생산을 실시하였다. 5 mM의 프럭토스를 동량의 ATP (Adenosine triphosphate)와 사카로마이세스 세레비지애(Saccharomyces cerevisea) 유래의 헥소카이네이즈(sigma-aldrich에서 구매)를 250 unit/ml로 하여 50 mM 트리즈마 베이스 완충용액에서 pH 7.5, 온도 30℃에서 60분간 반응을 수행하여 프럭토스 6-포스페이트를 생산한 결과 5mM의 프럭토스를 5mM의 프럭토스 6-포스페이트로 100% 전환하였으며 연속반응으로 0.5 unit/ml의 프럭토스 1,6-비스포스페이트 알돌레이즈 효소가 함유된 50 mM 트리즈마 베이스 완충용액을 pH 8.5에서 30분간 반응을 시킨 결과 5mM의 프럭토스 6-포스페이트가 4.65mM의 타가토스 6-포스페이트로 93% 전환되었다. 이 후 50unit/ml의 피타아제 효소(제노포커스, GenoPos TM 10000P)가 함유된 50 mM 트리즈마 베이스 완충용액에서 pH 5.5, 온도 60℃에서 60분간 반응을 수행한 결과 4.65mM의 타가토스 6-포스페이트가 4.65mM의 타가토스로 100% 전환되었다. 결론적으로 5mM의 프럭토스를 이용하여 헥소카이네이즈, 알돌레이즈, 피타아제의 칵테일반응을 이용한 결과 4.65mM의 타가토스로 93% 전환하는데 성공하였다.
Based on the results of the above examples, the production of tagatose was carried out from fructose using a cocktail reaction of fructokinase, aldolase, and phytase. The fructose of 5 mM in the same amount of ATP (Adenosine triphosphate) and Saccharomyces My process three Levy jiae (Saccharomyces The production of fructose 6-phosphate was carried out by reacting 50 mM Tris-base buffer at pH 7.5 and 30 ° C. for 60 minutes at a concentration of 250 unit / ml of hexokinase (purchased from Sigma-Aldrich) 5 mM fructose was converted to 100 mM of 5 mM fructose 6-phosphate and a 50 mM triazmase buffer solution containing 0.5 unit / ml of fructose 1,6-bisphosphate aldolase enzyme was added at a pH of 8.5 After 30 minutes of reaction, 5mM of fructose 6-phosphate was converted to 4.65mM of tagatose 6-phosphate by 93%. The reaction was then carried out in a 50 mM triazmase buffer solution containing 50 units / ml of phytase enzyme (Genopos, 10000P) at pH 5.5 and 60 ° C for 60 minutes. As a result, 4.65 mM of tagatose 6-phosphate 100% conversion to 4.65 mM tagatose. As a result, the cocktail reaction of hexokinase, aldolase, and phytase was performed using 5 mM fructose, resulting in 93% conversion to 4.65 mM tagatose.

실시예Example 13.  13. 프럭토스Fructose 1,6- 1,6- 비스포스페이트Bisphosphate 알돌레이즈Aldol Reyes 고정화 효소를 이용한  Using immobilized enzyme 타가토스Tagatos 생산 production

효소를 고정화시키기 위한 담체로 알긴산나트륨을 선택하고, 2.0, 2.5, 3.0%의 알긴산나트륨 수용액을 각각 제조하였다. 상기와 같이 다양한 농도로 제조된 알긴산나트륨 수용액을 각각 10㎖씩 취하여 효소액 10㎖과 각각 혼합한 후, 상기 혼합액을 주사기 펌프와 진공 펌프를 사용하여 0.2M 칼슘이온 용액에 떨어뜨려 비드가 생성되도록 하였다. 상기 효소-담체의 결합체 비드를 0.2% 글루타르알데하이드에서 1시간 동안 처리하여 가교시켰다.Sodium alginate was selected as a carrier for immobilizing the enzyme, and 2.0, 2.5, and 3.0% sodium alginate aqueous solutions were respectively prepared. 10 ml of each of the sodium alginate aqueous solutions prepared at various concentrations as described above was taken and mixed with 10 ml of the enzyme solution, and the mixture was dropped into 0.2 M calcium ion solution using a syringe pump and a vacuum pump to generate beads . The bead of the enzyme-carrier conjugate was cross-linked by treatment with 0.2% glutaraldehyde for 1 hour.

기질 용액은 pH 7.0의 50mM 트리스 완충 용액에 프럭토스 6-포스페이트가 10g/ℓ의 농도로 존재하는 용액을 사용하였으며, 생물반응기에 기질 용액을 넣은 후 고정화된 효소를 40 U 첨가하여 적절한 온도를 유지시키면서 반응시켰다. 이때 효소 역가 1 U(unit)는 60℃, pH 7에서 1분간 타가토스 1마이크로몰(μmole)을 생산할 수 있는 효소 양으로 정의하였다. 기질이 고갈될 때마다 새로 기질을 공급하기 위해, 3시간마다 기질 용액을 새것으로 갈아주었으며, 이를 8회 반복하여 장기간 반응 시에도 효소의 활성이 유지되는지를 관찰하고 그 결과를 하기 표 4에 나타내었다.The substrate solution used was a solution of fructose 6-phosphate at a concentration of 10 g / l in a 50 mM Tris buffer solution at pH 7.0. After adding the substrate solution to the bioreactor, 40 U of the immobilized enzyme was added to maintain the proper temperature Lt; / RTI > At this time, 1 U of enzyme was defined as the amount of enzyme capable of producing 1 micromole of tagatose at 60 ° C and pH 7 for 1 minute. In order to feed the new substrate every time the substrate was exhausted, the substrate solution was changed to a new one every 3 hours, and it was repeated 8 times to observe whether the enzyme activity was maintained even during long-term reaction. The results are shown in Table 4 .

(단위: g/l)(Unit: g / l) 교환횟수Number of exchanges 알긴산 나트륨 농도(%)Sodium alginate concentration (%) 2.02.0 2.52.5 3.03.0 1One 5.65.6 9.09.0 8.58.5 22 5.85.8 9.29.2 8.38.3 33 5.45.4 9.19.1 8.68.6 44 5.55.5 8.98.9 8.48.4 55 5.75.7 9.09.0 8.78.7 66 5.45.4 9.29.2 8.88.8 77 5.65.6 9.19.1 8.58.5 88 5.75.7 9.29.2 8.68.6

상기 표 4에서 알 수 있는 바와 같이, 알긴산 나트륨 농도는 2.5%가 가장 적합하였으며, 교환횟수 8회까지도 프럭토스 1,6-비스포스페이트 알돌레이즈 고정화 효소의 활성이 유지되었다.
As can be seen from Table 4, the sodium alginate concentration was most favorable at 2.5%, and the activity of the fructose 1,6-bisphosphate aldolase immobilized enzyme was maintained up to 8 times.

실시예Example 14.  14. 프럭토스Fructose 1,6- 1,6- 비스포스페이트Bisphosphate 알돌레이즈Aldol Reyes 발현 세포의 고정화를 이용한  Immobilization of expression cells 프럭토스Fructose 6- 6- 포스페이트의Phosphate 타가토스Tagatos 6- 6- 포스페이트로의Phosphate 전환 transform

액체 배지를 이용하여 배양하고 수확한 세포를 5000rpm에서 15분간 원심분리하여 증류수로 2회 세척한 뒤 2.5%(w/v)의 알지네이트 용액과 습세포 농도가 최종적으로 30%가 되게 충분히 혼합한 다음, 페리스탈틱 펌프에 연결된 시린지를 통해 0.5-3M의 염화칼슘 수용액 상에 적하시켜 비드(Bead) 형태의 고정화 세포를 얻었다. 얻어진 고정화 세포는 20-30℃에서 2-3시간 방치한 뒤, 0-8℃에서 16시간 동안 강화시킨다. 이때 시린지 게이지의 크기를 바꾸어 줌으로써 비드의 크기를 변화시킬 수가 있으며, 이에 따른 유효 인자를 구하였다. 유효 인자는 2%(w/v)로 알지네이트 내에 습세포를 최종농도 20%(w/v)로 포괄시켜 고정화 세포를 제조하고, 이 제조된 고정화 세포 5 unit를 pH 8.5, 50℃에서 50mM 프럭토스 6-포스페이트 용액 1리터와 60분간 반응시켜 생성되는 타가토스 6-포스페이트의 양을 측정하여 표 5에 나타내었다.The cells were cultured using a liquid medium and harvested. The cells were centrifuged at 5000 rpm for 15 minutes and washed twice with distilled water. 2.5% (w / v) alginate solution and wet cell concentration were mixed to a final concentration of 30% , And dropped onto 0.5-3 M calcium chloride aqueous solution through a syringe connected to a peristaltic pump to obtain immobilized cells in the form of beads. The immobilized cells thus obtained are allowed to stand at 20-30 ° C for 2-3 hours and then strengthened at 0-8 ° C for 16 hours. At this time, the size of the bead can be changed by changing the size of the syringe gauge, and the effective factors are obtained. The immobilized cells were prepared by entrapping wet cells in alginate at a final concentration of 20% (w / v) with an effective factor of 2% (w / v), and 5 units of the prepared immobilized cells were suspended in 50 mM The amount of tagatose 6-phosphate produced by reacting with 1 liter of the tosse 6-phosphate solution for 60 minutes was measured and is shown in Table 5.

경과시간(분)Elapsed time (minutes) 1010 2020 3030 4040 5050 6060 타가토스 생성량(mM)Tagatose yield (mM) 66 1818 2626 3434 3939 4545

실시예Example 15. 고정화 효소 및 연속반응기를 이용한  15. Immobilization Enzyme and Continuous Reactor 타가토스Tagatos 생산 production

고정화된 프럭토카이네이즈 또는 헥소카이네이즈 및 프럭토스 1,6-비스포스페이트 알돌레이즈 각각의 효소를 각각 고정화한 후 연속반응으로 프럭토스로부터 타가토스를 생산하고자 한다. 각각의 효소를 고정화시키기 위한 담체로 알긴산나트륨을 선택하고, 2.5%의 알긴산나트륨 수용액을 각각 제조하였다. 상기와 같이 제조된 알긴산나트륨 수용액을 각각 10㎖씩 취하여 효소액 10㎖과 각각 혼합한 후, 상기 혼합액을 주사기 펌프와 진공 펌프를 사용하여 0.2M 칼슘이온 용액에 떨어뜨려 비드가 생성되도록 하였다. 상기 효소-담체의 결합체 비드를 0.2% 글루타르알데하이드에서 1시간 동안 처리하여 가교시켰다.Immobilized fructokinase or hexokinase, and fructose 1,6-bisphosphate aldolase, respectively, followed by sequential reactions to produce tagatose from fructose. Sodium alginate was selected as a carrier for immobilizing each enzyme, and a 2.5% sodium alginate aqueous solution was prepared. 10 ml of each of the sodium alginate aqueous solutions prepared as described above was taken and mixed with 10 ml of the enzyme solution. The mixture was dropped into 0.2 M calcium ion solution using a syringe pump and a vacuum pump to generate beads. The bead of the enzyme-carrier conjugate was cross-linked by treatment with 0.2% glutaraldehyde for 1 hour.

첫 단계인 프럭토스 6-포스페이트 생산을 위한 반응에서, 기질 용액은 pH 7.0의 50mM 트리스 완충 용액에 50mM 프럭토스와 동량의 ATP를 녹여 제조한 용액을 사용하였으며, 생물반응기에 기질 용액을 넣은 후 고정화된 효소를 40 U 첨가하여 적절한 온도를 유지시키면서 반응시켰다. 타가토스 6-포스페이트 생산을 위한 두 번째 반응을 위해서 1차 반응물의 pH를 8.5로 조절하고, 고정화효소 40U를 첨가하여 50분간 반응시켰다. 마지막으로, 타가토스 생산을 위해서는 2차 반응물의 pH를 5.5로 조정하고 60℃에서 60분간 반응시켜 생성되는 타가토스의 양을 분석하여 하기 표 6에 나타내었다.In the first step of the reaction for the production of fructose 6-phosphate, a solution prepared by dissolving 50 mM fructose and the same amount of ATP in a 50 mM Tris buffer solution of pH 7.0 was used. The substrate solution was added to the bioreactor, 40 [mu] l of the enzyme was added and reacted at an appropriate temperature. For the second reaction for the production of tagatose 6-phosphate, the pH of the first reaction product was adjusted to 8.5, 40 U of immobilized enzyme was added, and the reaction was carried out for 50 minutes. Finally, for the production of tagatose, the pH of the second reactant was adjusted to 5.5 and reacted at 60 DEG C for 60 minutes to analyze the amount of tagatose produced. The results are shown in Table 6 below.

경과시간(분)Elapsed time (minutes) 1010 2020 3030 4040 5050 6060 타가토스 생성량(mM)Tagatose yield (mM) 55 1717 2424 3333 4141 4747

<110> CHEBIGEN INC. <120> Composition for production of tagatose from fructose using enzyme combination reaction with substrates and uses thereof <130> PN13297 <160> 93 <170> KopatentIn 2.0 <210> 1 <211> 354 <212> PRT <213> Alcanivorax borkumensis <400> 1 Met Ala Leu Val Ser Met Arg Gln Leu Leu Asp His Ala Ala Glu Phe 1 5 10 15 Gly Tyr Gly Val Pro Ala Tyr Asn Val Asn Asn Val Glu Gln Met Arg 20 25 30 Ala Ile Met Glu Ala Ala Asp Lys Thr Asn Ser Pro Val Ile Val Gln 35 40 45 Ala Ser Ala Gly Ala Arg Lys Tyr Ala Gly Ala Pro Phe Leu Arg His 50 55 60 Leu Ile Leu Ala Ala Thr Glu Glu Phe Pro His Ile Pro Val Val Met 65 70 75 80 His Gln Asp His Gly Thr Ser Pro Ser Val Cys Gln Arg Ser Ile Gln 85 90 95 Leu Gly Phe Ser Ser Val Met Met Asp Gly Ser Leu Gly Glu Asp Gly 100 105 110 Lys Thr Pro Thr Asp Tyr Glu Tyr Asn Val Glu Val Thr Arg Arg Thr 115 120 125 Val Asp Met Ala His Ala Cys Gly Val Ser Val Glu Gly Glu Leu Gly 130 135 140 Cys Leu Gly Ser Leu Glu Thr Gly Glu Ala Gly Glu Glu Asp Gly Ile 145 150 155 160 Gly Ala Ala Gly Lys Leu Thr His Glu Gln Met Leu Thr Asp Pro Glu 165 170 175 Glu Ala Ala Asp Phe Val Gln Lys Thr Lys Val Asp Ala Leu Ala Ile 180 185 190 Ala Ile Gly Thr Ser His Gly Ala Tyr Lys Phe Thr Arg Pro Pro Thr 195 200 205 Gly Asp Ile Leu Ala Ile Glu Gln Ile Lys Ala Ile His Lys Arg Ile 210 215 220 Pro Asp Thr His Leu Val Met His Gly Ser Ser Ser Val Pro Gln Asp 225 230 235 240 Trp Leu Ala Ile Ile Asn Glu Phe Gly Gly Glu Ile Pro Glu Thr Tyr 245 250 255 Gly Val Pro Val Glu Glu Ile Gln Glu Gly Ile Lys His Gly Val Arg 260 265 270 Lys Val Asn Ile Asp Thr Asp Leu Arg Leu Ala Ser Thr Gly Ala Ile 275 280 285 Arg Arg Phe Leu Ala Gln Asn Pro Ala Glu Phe Asp Pro Arg Lys Tyr 290 295 300 Leu Lys Glu Ser Ile Thr Ala Met Arg Asp Ile Cys Met Ala Arg Tyr 305 310 315 320 Glu Ala Phe Gly Thr Ala Gly Tyr Ala Asp Lys Ile Lys Pro Ile Ser 325 330 335 Leu Glu Lys Met Phe Gln Arg Tyr Glu Ser Gly Asp Leu Asp Pro Arg 340 345 350 Val Lys <210> 2 <211> 287 <212> PRT <213> Bacillus alcalophilus <400> 2 Met Pro Leu Val Ser Met Thr Glu Met Leu Asn Lys Ala Lys Ala Glu 1 5 10 15 Gly Tyr Ala Val Gly Gln Phe Asn Leu Asn Asn Leu Glu Phe Thr Gln 20 25 30 Ala Ile Leu Gln Ala Ala Glu Glu Glu Lys Ser Pro Val Ile Leu Gly 35 40 45 Val Ser Glu Gly Ala Ala Arg Tyr Met Gly Gly Phe Lys Thr Val Val 50 55 60 Ser Met Val Lys Ala Leu Met Asp Glu Tyr Asn Val Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Leu Glu Lys Cys Val Gln Ala 85 90 95 Ile His Ala Gly Phe Thr Ser Val Met Ile Asp Gly Ser His His Pro 100 105 110 Leu Ala Glu Asn Ile Ala Ile Thr Lys Arg Val Val Glu Ile Ala His 115 120 125 Ala Val Gly Val Ser Val Glu Ala Glu Leu Gly Arg Ile Gly Gly Gln 130 135 140 Glu Asp Asp Leu Ile Val Asp Asp Ala Glu Ala Ala Tyr Ala Ile Pro 145 150 155 160 Ala Glu Cys Lys Glu Leu Val Ala Ala Thr Gly Val Asp Cys Phe Ala 165 170 175 Pro Ala Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Asn Leu 180 185 190 Gly Phe Asp His Met Lys Glu Ile Asp Ser Leu Val Gly Ile Pro Leu 195 200 205 Val Leu His Gly Gly Thr Gly Ile Pro Thr Ala Asp Ile Lys Lys Ala 210 215 220 Ile Glu Phe Gly His Ala Lys Ile Asn Val Asn Thr Glu Ser Gln Ile 225 230 235 240 Ser Ser Ala Lys Ala Val Arg Glu Val Leu Ala Ala Asp Thr Asp Val 245 250 255 Tyr Asp Pro Arg Lys Tyr Leu Gly Pro Ala Arg Glu Ala Ile Lys Glu 260 265 270 Thr Val Lys Gly Lys Met Lys Glu Phe Gly Ser Ser Asn Arg Ala 275 280 285 <210> 3 <211> 285 <212> PRT <213> Bacillus amyloliquefaciens <400> 3 Met Pro Leu Val Ser Met Thr Glu Met Leu Asn Thr Ala Lys Glu Lys 1 5 10 15 Gly Tyr Ala Val Gly Gln Phe Asn Leu Asn Asn Leu Glu Phe Thr Gln 20 25 30 Ala Ile Leu Gln Ala Ala Gln Glu Glu Lys Ser Pro Val Ile Leu Gly 35 40 45 Val Ser Glu Gly Ala Gly Arg Tyr Met Gly Gly Phe Lys Thr Val Val 50 55 60 Ala Met Val Lys Ala Leu Met Glu Glu Tyr Lys Val Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Ser Cys Ala Lys Ala 85 90 95 Ile His Ala Gly Phe Thr Ser Val Met Ile Asp Ala Ser His His Pro 100 105 110 Phe Glu Glu Asn Val Ala Thr Thr Ala Lys Val Val Glu Leu Ala His 115 120 125 Phe His Gly Val Ser Val Glu Ala Glu Leu Gly Thr Val Gly Gly Gln 130 135 140 Glu Asp Asp Val Ile Ala Glu Gly Val Ile Tyr Ala Asp Pro Lys Glu 145 150 155 160 Cys Gln Glu Leu Val Glu Arg Thr Gly Ile Asp Cys Leu Ala Pro Ala 165 170 175 Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Asn Leu Gly Phe 180 185 190 Lys Glu Met Glu Asp Ile Gly Lys Thr Thr Gly Leu Pro Leu Val Leu 195 200 205 His Gly Gly Thr Gly Ile Pro Thr Ala Asp Ile Lys Lys Ser Ile Ser 210 215 220 Leu Gly Thr Ala Lys Ile Asn Val Asn Thr Glu Asn Gln Ile Ser Ser 225 230 235 240 Ala Lys Ala Val Arg Glu Thr Leu Ala Ala Lys Pro Asn Glu Tyr Asp 245 250 255 Pro Arg Lys Tyr Leu Gly Pro Ala Arg Glu Ala Ile Lys Glu Thr Val 260 265 270 Ile Gly Lys Met Arg Glu Phe Gly Ser Ser Asn Gln Ala 275 280 285 <210> 4 <211> 287 <212> PRT <213> Bacillus azotoformans <400> 4 Met Pro Leu Val Ser Met Thr Glu Met Leu Asn Lys Ala Lys Asn Glu 1 5 10 15 Gly Tyr Ala Val Gly Gln Phe Asn Ile Asn Asn Leu Glu Tyr Thr Gln 20 25 30 Ala Ile Leu Gln Ala Ala Gln Ala Glu Asn Ser Pro Val Ile Leu Gly 35 40 45 Val Ser Glu Gly Ala Ala Lys Tyr Met Gly Gly Phe Lys Thr Val Val 50 55 60 Ala Met Val Lys Gly Leu Met Glu Tyr Tyr Glu Met Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Lys Cys Val Glu Ala 85 90 95 Met Tyr Ala Gly Phe Thr Ser Val Met Ile Asp Gly Ser His Tyr Pro 100 105 110 Leu Glu Glu Asn Ile Glu Leu Thr Lys Arg Val Val Asp Val Ala His 115 120 125 Thr Ile Gly Ile Ser Val Glu Ala Glu Leu Gly Arg Ile Gly Gly Gln 130 135 140 Glu Asp Asp Leu Val Val Asp Asp Ala Glu Ala Met Tyr Ala Ile Pro 145 150 155 160 Ala Glu Cys Asp Arg Leu Val Arg Glu Thr Gly Val Asp Cys Phe Ala 165 170 175 Pro Ala Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Lys Leu 180 185 190 Gly Phe Asp Arg Met Lys Glu Val Met Glu Leu Thr Gly Val Pro Leu 195 200 205 Val Leu His Gly Gly Thr Gly Ile Pro Thr Lys Asp Ile Gln Lys Ala 210 215 220 Ile Ser Leu Gly Thr Ala Lys Ile Asn Val Asn Thr Glu Asn Gln Ile 225 230 235 240 Thr Ser Thr Lys Thr Val Arg Lys Val Leu Glu Glu Lys Pro Glu Leu 245 250 255 Tyr Asp Thr Arg Lys Phe Met Gly Pro Ala Arg Asp Thr Ile Lys Glu 260 265 270 Thr Val Ile Gly Lys Ile Arg Glu Phe Gly Ser Ser Asn Lys Ala 275 280 285 <210> 5 <211> 287 <212> PRT <213> Bacillus cellulosilyticus <400> 5 Met Pro Leu Val Ser Met Lys Glu Met Leu Glu Asp Ala Lys Ala Gly 1 5 10 15 Gly Tyr Ala Val Gly Gln Phe Asn Leu Asn Asn Leu Glu Phe Thr Gln 20 25 30 Ala Ile Leu Leu Ala Ala Glu Glu Glu Gln Ser Pro Val Ile Leu Gly 35 40 45 Val Ser Glu Gly Ala Ala Arg Tyr Met Gly Gly Phe Leu Thr Val Val 50 55 60 Ser Ile Val Glu Ala Leu Met Ala Asp Tyr Glu Ile Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Lys Cys Val Glu Ala 85 90 95 Ile His Ala Gly Phe Thr Ser Val Met Ile Asp Gly Ser His His Pro 100 105 110 Leu Glu Glu Asn Ile Ala Leu Thr Lys Gln Val Val Ala Val Ala His 115 120 125 Thr Leu Gly Val Ser Val Glu Ala Glu Leu Gly Arg Ile Gly Gly Gln 130 135 140 Glu Asp Asp Leu Val Val Asp Asp Ala Asp Ala Ala Tyr Ala Ile Pro 145 150 155 160 Ser Glu Cys Asp Gln Leu Val Arg Glu Thr Gly Val Asp Cys Phe Ala 165 170 175 Pro Ala Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Asn Leu 180 185 190 Gly Phe Asp Arg Met Glu Glu Val Ala Asn Leu Thr Gly Val Pro Leu 195 200 205 Val Leu His Gly Gly Thr Gly Ile Pro Thr His Asp Ile Gln Lys Ala 210 215 220 Ile Ser Leu Gly Thr Ala Lys Ile Asn Val Asn Thr Glu Asn Gln Ile 225 230 235 240 Ser Ser Ala Lys Arg Val Arg Glu Val Leu Ala Glu Lys Pro Asn Glu 245 250 255 Tyr Asp Pro Arg Lys Tyr Leu Gly Pro Ala Arg Asp Ala Ile Lys Glu 260 265 270 Thr Val Ile Gly Lys Met Arg Glu Phe Gly Ser Ser Asn Lys Ala 275 280 285 <210> 6 <211> 285 <212> PRT <213> Bacillus coagulans <400> 6 Met Pro Leu Val Ser Met Thr Glu Met Leu Asn Lys Ala Lys Glu Gly 1 5 10 15 Lys Tyr Ala Val Gly Gln Phe Asn Val Asn Asn Leu Glu Tyr Ala Gln 20 25 30 Ala Ile Leu Met Ala Ala Glu Glu Glu Lys Ser Pro Val Ile Leu Gly 35 40 45 Val Ser Glu Gly Ala Gly Arg Tyr Met Ser Gly Phe Lys Thr Val Val 50 55 60 Lys Met Val Glu Gly Leu Met Glu Asp Leu Asn Val Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Asn Ala Lys Lys Ala 85 90 95 Ile Asp Ala Gly Phe Thr Ser Val Met Ile Asp Ala Ser His His Pro 100 105 110 Phe Asp Glu Asn Val Glu Ile Thr Ser Lys Val Val Glu Tyr Ala His 115 120 125 Ala His Gly Val Ser Val Glu Ala Glu Leu Gly Thr Val Gly Gly Gln 130 135 140 Glu Asp Asp Val Val Gly Lys Gly Ile Ile Tyr Ala Asp Pro Glu Glu 145 150 155 160 Cys Lys Lys Leu Val Asp Ala Thr Lys Ile Asp Ala Leu Ala Pro Ala 165 170 175 Leu Gly Ser Val His Gly Pro Tyr His Gly Glu Pro Asn Leu Gly Phe 180 185 190 Lys Glu Met Glu Glu Ile Ser Lys Ile Thr Gly Met Pro Leu Val Leu 195 200 205 His Gly Gly Thr Gly Ile Pro Glu Ala Asp Ile Lys Lys Ala Ile Ser 210 215 220 Leu Gly Thr Ala Lys Ile Asn Val Asn Thr Glu Asn Gln Ile Ala Ser 225 230 235 240 Ala Lys Thr Val Arg Glu Val Leu Ala Ala Asp Pro Asp Val Tyr Asp 245 250 255 Pro Arg Lys Tyr Leu Gly Pro Ala Arg Glu Thr Ile Lys Gln Thr Val 260 265 270 Ile Gly Lys Ile Arg Glu Phe Gly Ser Asn Gln Lys Ala 275 280 285 <210> 7 <211> 285 <212> PRT <213> Bacillus cytotoxis <400> 7 Met Pro Leu Val Ser Met Lys Glu Met Leu Asn Lys Ala Leu Glu Gly 1 5 10 15 Lys Tyr Ala Val Gly Gln Phe Asn Ile Asn Asn Leu Glu Trp Thr Gln 20 25 30 Ala Ile Leu Ala Ala Ala Glu Glu Glu Lys Ser Pro Val Ile Leu Gly 35 40 45 Val Ser Glu Gly Ala Ala Arg His Met Thr Gly Phe Lys Thr Val Val 50 55 60 Ala Met Val Lys Ala Leu Ile Glu Glu Met Asn Ile Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Lys Cys Lys Glu Ala 85 90 95 Ile Asp Ala Gly Phe Thr Ser Val Met Ile Asp Ala Ser His His Pro 100 105 110 Phe Glu Glu Asn Val Glu Thr Thr Lys Lys Val Val Glu Tyr Ala His 115 120 125 Ala Arg Asn Val Ser Val Glu Ala Glu Leu Gly Thr Val Gly Gly Gln 130 135 140 Glu Asp Asp Val Ile Ala Asp Gly Ile Ile Tyr Ala Asp Pro Glu Glu 145 150 155 160 Cys Lys His Leu Val Glu Ala Thr Gly Ile Asp Cys Leu Ala Pro Ala 165 170 175 Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Asn Leu Gly Phe 180 185 190 Lys Glu Met Glu Gln Val Arg Asp Leu Thr Gly Val Pro Leu Val Leu 195 200 205 His Gly Gly Thr Gly Ile Pro Thr Ala Asp Ile Lys Lys Ala Ile Ser 210 215 220 Leu Gly Thr Ser Lys Ile Asn Val Asn Thr Glu Asn Gln Ile Ala Phe 225 230 235 240 Thr Lys Ala Val Arg Glu Val Leu Asn Lys Asp Gln Glu Val Tyr Asp 245 250 255 Pro Arg Lys Phe Ile Gly Pro Gly Arg Glu Ala Ile Lys Ala Thr Val 260 265 270 Ile Gly Lys Ile Arg Glu Phe Gly Ser Asn Gly Lys Ala 275 280 285 <210> 8 <211> 287 <212> PRT <213> Bacillus halodurans <400> 8 Met Pro Leu Val Ser Met Thr Glu Met Leu Asn Lys Ala Lys Ala Glu 1 5 10 15 Gly Tyr Ala Val Gly Gln Phe Asn Leu Asn Asn Leu Glu Phe Thr Gln 20 25 30 Ala Ile Leu Gln Ala Ala Glu Glu Glu Lys Ser Pro Val Ile Cys Gly 35 40 45 Val Ser Glu Gly Ala Ala Arg Tyr Met Gly Gly Phe Lys Thr Val Val 50 55 60 Gly Met Val Lys Asn Leu Met Gln Glu Tyr Asn Val Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Lys Cys Val Glu Ala 85 90 95 Ile His Ala Gly Phe Thr Ser Val Met Ile Asp Gly Ser His His Pro 100 105 110 Leu Glu Glu Asn Ile Ala Leu Thr Lys Arg Val Val Asp Val Ala His 115 120 125 Ala Leu Gly Val Ser Val Glu Ala Glu Leu Gly Arg Ile Gly Gly Gln 130 135 140 Glu Asp Asp Leu Val Val Asp Glu Ala Glu Ala Ala Tyr Ala Ile Pro 145 150 155 160 Ser Glu Cys Lys Glu Leu Val Glu Ala Thr Gly Val Asp Cys Phe Ala 165 170 175 Pro Ala Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Asn Leu 180 185 190 Gly Phe Asp Arg Met Lys Glu Ile Asn Glu Leu Val Gly Ile Pro Leu 195 200 205 Val Leu His Gly Gly Thr Gly Ile Pro Thr Lys Asp Ile Gln Lys Ala 210 215 220 Ile Ser Leu Gly His Ala Lys Ile Asn Val Asn Thr Glu Ser Gln Ile 225 230 235 240 Ser Ser Ala Lys Ala Val Arg Gln Val Leu Glu Glu Lys Pro Glu Leu 245 250 255 Tyr Asp Pro Arg Lys Tyr Leu Gly Pro Ala Arg Glu Ala Ile Lys Asp 260 265 270 Thr Val Lys Gly Lys Met Arg Glu Phe Gly Ser Ser Asn Lys Ala 275 280 285 <210> 9 <211> 283 <212> PRT <213> Bacillus licheniformis <400> 9 Met Leu Thr Asn Thr Lys Lys Met Leu Leu Asp Ala Gln Ala Asn Gly 1 5 10 15 Tyr Ala Val Pro Ala Phe Asn Ile His Asn Leu Glu Thr Val Gln Ala 20 25 30 Val Thr Glu Ala Ala Glu Ala Leu Arg Ser Pro Val Ile Leu Ala Ala 35 40 45 Thr Pro Gly Thr Phe Ser Tyr Ala Gly Arg Asp Tyr Ile Gln Ala Ile 50 55 60 Ala Glu Thr Ala Ala Ala Arg Cys Ser Ile Pro Val Ala Leu His Leu 65 70 75 80 Asp His His Glu Glu Ile Asp Asp Ile Arg Glu Ser Leu Ser Leu Gly 85 90 95 Thr Lys Ser Val Met Ile Asp Ala Ser His His Pro Phe Ala Glu Asn 100 105 110 Thr Ala Ile Thr Ala Ser Val Val Gln Tyr Ala Arg Gln Lys Asp Ala 115 120 125 Thr Val Glu Ala Glu Leu Gly Arg Leu Gly Gly Gln Glu Asp Asp Leu 130 135 140 Ile Val Asp Glu Ala Asp Ala Tyr Tyr Thr Asp Pro Asp Ala Ala Lys 145 150 155 160 Glu Phe Val Glu Lys Thr Gly Ile Asp Ser Leu Ala Val Ala Ile Gly 165 170 175 Thr Ala His Gly Leu Tyr Ser Lys Glu Pro Asn Ile Asp Phe Glu Arg 180 185 190 Leu Ala Ala Ile Gln Lys Arg Val Ser Val Pro Leu Val Leu His Gly 195 200 205 Ala Ser Gly Ile Ser Glu Gln Asp Val Arg Lys Cys Ile Lys Leu Gly 210 215 220 Cys Ala Lys Val Asn Ile Ala Thr Glu Leu Lys Ile Pro Phe Ser Asp 225 230 235 240 Lys Leu Arg Arg Tyr Leu His Glu His Ala Asp Ala Ser Asp Pro Arg 245 250 255 Lys Tyr Met Ala Pro Ala Lys Glu Ala Met Lys Lys Ile Val Gln Glu 260 265 270 Lys Ile Leu Met Cys Met Ser Asn Asp Arg Tyr 275 280 <210> 10 <211> 287 <212> PRT <213> Bacillus macauensis <400> 10 Met Pro Leu Val Ser Met Lys Glu Met Leu Glu Lys Gly Lys Ala Glu 1 5 10 15 Gly Tyr Ala Val Gly Gln Phe Asn Ile Asn Asn Leu Glu Phe Thr Gln 20 25 30 Ala Ile Leu Lys Ala Ala Gln Glu Glu Asn Ser Pro Val Ile Leu Gly 35 40 45 Val Ser Glu Gly Ala Ala Arg Tyr Met Gly Gly Phe Lys Leu Val Val 50 55 60 Ala Met Thr Lys Ala Leu Met Glu Glu Tyr Ser Ile Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Asn Cys Ala Lys Ala 85 90 95 Ile His Ala Gly Phe Thr Ser Val Met Ile Asp Gly Ser His Phe Pro 100 105 110 Leu Glu Glu Asn Ile Ala Leu Thr Lys Lys Val Val Glu Leu Ala His 115 120 125 Ile His Gly Val Ser Val Glu Ala Glu Leu Gly Arg Ile Gly Gly Gln 130 135 140 Glu Asp Asp Leu Ile Val Asp Asp Ala Asp Ala Ala Tyr Ala Ile Pro 145 150 155 160 Ser Glu Cys Asp Gln Leu Val Arg Glu Thr Gly Val Asp Cys Phe Ala 165 170 175 Pro Ala Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Asn Leu 180 185 190 Gly Phe Asp Arg Met Lys Glu Val Asn Glu Leu Thr Gly Val Pro Leu 195 200 205 Val Leu His Gly Gly Thr Gly Ile Pro Thr His Asp Ile Gln Lys Ala 210 215 220 Ile Ser Leu Gly Thr Ala Lys Ile Asn Val Asn Thr Glu Ser Gln Ile 225 230 235 240 Ser Ser Phe Asn Ala Ile Asn Glu Phe Ile Ala Ala Asn Pro Asp Lys 245 250 255 Tyr Asp Pro Arg Lys Tyr Leu Thr Ala Gly Arg Glu Ala Ile Leu Ala 260 265 270 Thr Val Lys Ala Lys Met Arg Glu Phe Gly Ser Ser Asn Lys Ala 275 280 285 <210> 11 <211> 285 <212> PRT <213> Bacillus megaterium <400> 11 Met Pro Leu Val Ser Met Lys Glu Met Leu Ile Thr Ala Lys Glu Gln 1 5 10 15 Gly Tyr Ala Val Gly Gln Phe Asn Leu Asn Asn Leu Glu Phe Thr Gln 20 25 30 Ala Ile Leu Gln Ala Ala Lys Glu Glu Asn Ser Pro Val Ile Leu Gly 35 40 45 Val Ser Glu Gly Ala Ala Arg Tyr Met Gly Gly Phe Lys Thr Val Val 50 55 60 Ala Met Val Glu Ala Leu Ile Glu Asp Tyr Asp Val Gln Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Ser Cys Ala Lys Ala 85 90 95 Ile His Ala Gly Phe Thr Ser Val Met Ile Asp Ala Ser His His Pro 100 105 110 Phe Glu Glu Asn Ile Ala Ile Thr Ser Lys Val Val Glu Leu Ala His 115 120 125 Phe His Gly Val Ser Val Glu Ala Glu Leu Gly Thr Val Gly Gly Gln 130 135 140 Glu Asp Asp Val Ile Ala Glu Gly Val Ile Tyr Ala Asp Ala Asn Glu 145 150 155 160 Cys Glu Glu Leu Val Asn Arg Thr Gly Ile Asp Cys Leu Ala Pro Ala 165 170 175 Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Asn Leu Gly Phe 180 185 190 Ala Glu Met Lys Glu Ile Gly Asp Arg Thr Gly Leu Pro Leu Val Leu 195 200 205 His Gly Gly Thr Gly Ile Pro Thr Lys Asp Ile Gln Lys Ser Val Ser 210 215 220 Leu Gly Thr Ala Lys Ile Asn Val Asn Thr Glu Asn Gln Ile Ala Ser 225 230 235 240 Ala Lys Ala Val Arg Glu Val Leu Ala Ala Gln Pro Glu Gln Tyr Asp 245 250 255 Pro Arg Lys Tyr Leu Gly Pro Ala Arg Glu Ala Ile Lys Glu Thr Val 260 265 270 Gln Gly Lys Met Arg Glu Phe Gly Ser Ser Asn Lys Ala 275 280 285 <210> 12 <211> 284 <212> PRT <213> Bacillus methanolicus <400> 12 Met Pro Leu Val Ser Met Lys Asp Met Leu Asn His Gly Lys Glu Asn 1 5 10 15 Gly Tyr Ala Val Gly Gln Phe Asn Ile Asn Asn Leu Glu Phe Gly Gln 20 25 30 Ala Ile Leu Gln Ala Ala Glu Glu Glu Lys Ser Pro Val Ile Ile Gly 35 40 45 Val Ser Val Gly Ala Ala Asn Tyr Met Gly Gly Phe Lys Leu Ile Val 50 55 60 Asp Met Val Lys Ser Leu Met Asp Ser Tyr Asn Val Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Pro Ser Leu Glu Lys Cys Val Gln Ala 85 90 95 Ile His Ala Gly Phe Thr Ser Val Met Ile Asp Gly Ser His Leu Pro 100 105 110 Leu Glu Glu Asn Ile Glu Leu Thr Lys Arg Val Val Glu Ile Ala His 115 120 125 Ser Val Gly Val Ser Val Glu Ala Glu Leu Gly Arg Ile Gly Gly Gln 130 135 140 Glu Asp Asp Val Val Ala Glu Ser Phe Tyr Ala Ile Pro Ser Glu Cys 145 150 155 160 Glu Gln Leu Val Arg Glu Thr Gly Val Asp Cys Phe Ala Pro Ala Leu 165 170 175 Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Lys Leu Gly Phe Asp 180 185 190 Arg Met Glu Glu Ile Met Lys Leu Thr Gly Val Pro Leu Val Leu His 195 200 205 Gly Gly Thr Gly Ile Pro Thr Lys Asp Ile Gln Lys Ala Ile Ser Leu 210 215 220 Gly Thr Ala Lys Ile Asn Val Asn Thr Glu Ser Gln Ile Ala Ala Thr 225 230 235 240 Lys Ala Val Arg Glu Val Leu Asn Asn Asp Ala Lys Leu Phe Asp Pro 245 250 255 Arg Lys Phe Leu Ala Pro Ala Arg Glu Ala Ile Lys Glu Thr Ile Lys 260 265 270 Gly Lys Met Arg Glu Phe Gly Ser Ser Gly Lys Ala 275 280 <210> 13 <211> 298 <212> PRT <213> Bacillus nealsonii <400> 13 Met Asn Asn Gln Leu Glu Lys Met Lys Thr Lys Lys Gly Phe Ile Ala 1 5 10 15 Ala Leu Asp Gln Ser Gly Gly Ser Thr Pro Lys Ala Leu Leu His Tyr 20 25 30 Gly Val Ala Glu Asn Thr Tyr Ala Asn Glu Glu Glu Met Phe Asp Leu 35 40 45 Val His Gln Met Arg Thr Arg Ile Ile Thr Ser Pro Ala Phe Asp Ser 50 55 60 Glu Tyr Ile Leu Gly Ala Ile Leu Phe Glu Gln Thr Met Asp Ser Lys 65 70 75 80 Ile Glu Gly Met Tyr Thr Ala Asp Tyr Leu Ala Glu Lys Lys Gly Ile 85 90 95 Val Pro Phe Leu Lys Ile Asp Lys Gly Leu Ala Glu Leu Thr Asn Gly 100 105 110 Val Gln Leu Met Lys Pro Ile Thr Asp Leu Asp Asp Ile Leu Arg Arg 115 120 125 Ala Lys Glu Arg His Ile Phe Gly Thr Lys Met Arg Ser Val Ile Lys 130 135 140 Glu Ala Asn Pro Glu Ser Ile Lys Ala Val Val Asp Gln Gln Phe Glu 145 150 155 160 Ile Gly Lys Gln Ile Ile Ala Ala Gly Leu Val Pro Ile Ile Glu Pro 165 170 175 Glu Val Asp Ile Asn Ser Pro Asp Lys Glu Gln Ser Glu Ala Ile Leu 180 185 190 Lys Gln Glu Leu Leu Lys His Leu His Asn Leu Lys Glu Asp Glu Asn 195 200 205 Val Met Leu Lys Leu Ser Ile Pro Thr Val Ala Asn Thr Tyr Lys Glu 210 215 220 Leu Thr Thr His Ser Arg Val Val Arg Val Val Ala Leu Ser Gly Gly 225 230 235 240 Tyr Ser Arg Glu Glu Ala Asn Gln Lys Leu Lys Glu Asn Asp Asn Ile 245 250 255 Ile Ala Ser Phe Ser Arg Ala Leu Ser Glu Glu Leu Asn Val Asn Gln 260 265 270 Ser Glu Glu Glu Phe Asn Asn Thr Leu Lys Glu Ala Val Thr Ser Ile 275 280 285 Tyr Glu Ala Ser Thr Ala Lys Thr Gly Arg 290 295 <210> 14 <211> 287 <212> PRT <213> Bacillus pseudofirmus <400> 14 Met Pro Leu Val Ser Met Lys Asp Met Leu Asn Lys Ala Lys Ala Glu 1 5 10 15 Gly Tyr Ala Val Gly Gln Phe Asn Leu Asn Asn Leu Glu Phe Thr Gln 20 25 30 Ala Ile Leu Gln Ala Ala Gln Glu Glu Asn Ser Pro Val Ile Cys Gly 35 40 45 Val Ser Glu Gly Ala Ala Arg Tyr Met Gly Gly Phe Lys Thr Val Val 50 55 60 Lys Met Val Glu Ser Leu Met Glu Glu Tyr Asn Val Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Lys Cys Val Glu Ala 85 90 95 Ile His Ala Gly Phe Thr Ser Val Met Ile Asp Gly Ser His Tyr Pro 100 105 110 Leu Glu Glu Asn Ile Ala Leu Thr Lys Arg Val Val Asp Val Ala His 115 120 125 Ala Leu Gly Val Ser Val Glu Ala Glu Leu Gly Arg Ile Gly Gly Gln 130 135 140 Glu Asp Asp Leu Ile Val Asp Asp Ala Glu Ala Ala Tyr Ala Ile Pro 145 150 155 160 Ser Glu Cys Lys Glu Leu Val Glu Ala Thr Gly Val Asp Cys Phe Ala 165 170 175 Pro Ala Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Asn Leu 180 185 190 Gly Phe Asp His Met Lys Glu Ile Asp Gly Leu Val Gly Ile Pro Leu 195 200 205 Val Leu His Gly Gly Thr Gly Ile Pro Thr Lys Asp Val Gln Lys Ala 210 215 220 Ile Glu Phe Gly His Ala Lys Ile Asn Val Asn Thr Glu Ser Gln Ile 225 230 235 240 Ala Ser Ala Lys Ala Val Arg Glu Thr Leu Ala Ala Lys Pro Asn Glu 245 250 255 Tyr Asp Pro Arg Lys Phe Leu Gly Pro Ala Arg Glu Ala Ile Lys Glu 260 265 270 Thr Val Gln Gly Lys Met Arg Glu Phe Gly Ser Ser Asn Lys Ala 275 280 285 <210> 15 <211> 284 <212> PRT <213> Bacillus selenitireducens <400> 15 Met Pro Leu Val Ser Met Thr Asp Met Leu Asn Lys Ala Phe Gln Asn 1 5 10 15 Gln Tyr Ala Val Gly Gln Phe Asn Ile Asn Asn Leu Glu Trp Thr Gln 20 25 30 Ala Ile Leu Gln Ala Ala Glu Glu Glu Gln Ser Pro Val Ile Leu Gly 35 40 45 Val Ser Glu Gly Ala Ala Lys Tyr Met Gly Gly Phe Lys Leu Ile Thr 50 55 60 Ala Met Val Lys Glu Leu Met Glu Ser Gln Gly Thr Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Gln Ser Ile Glu Asn Cys Lys Lys Ala 85 90 95 Val Asp Ala Gly Phe Thr Ser Val Met Ile Asp Gly Ser His His Pro 100 105 110 Ile Glu Glu Asn Ile Glu Met Thr Lys Ala Val Val Glu Tyr Ala His 115 120 125 Ala Arg Gly Ala Ser Val Glu Ala Glu Val Gly Thr Val Gly Gly Glu 130 135 140 Glu Asp Gly Val Ile Gly Gly Val Asn Tyr Ala Asp Leu Glu Glu Cys 145 150 155 160 Val Arg Ile Thr Lys Glu Gly Lys Ala Asp Ala Leu Ala Ala Ala Leu 165 170 175 Gly Ser Val His Gly Pro Tyr Gln Gly Glu Pro Lys Leu Gly Phe Lys 180 185 190 Glu Met Glu Glu Ile Ala Asn Ala Thr Lys Thr Pro Leu Val Leu His 195 200 205 Gly Gly Ser Gly Ile Pro Thr His Gln Val Lys Lys Ala Ile Glu Phe 210 215 220 Gly His Ala Lys Ile Asn Val Asn Thr Glu Cys Gln Ile Glu Trp Thr 225 230 235 240 Lys Ala Val Arg Glu Lys Leu Ala Asn Asp Glu Lys Val Tyr Asp Pro 245 250 255 Arg Lys Val Ile Gly Pro Gly Lys Glu Ala Ile Val Arg Val Val Lys 260 265 270 Asp Lys Met Gln Glu Phe Gly Ser Ser Asn Gln Ala 275 280 <210> 16 <211> 290 <212> PRT <213> Bacillus sp. <400> 16 Met Ala Phe Val Ser Met Lys Glu Leu Leu Glu Asp Ala Lys Arg Glu 1 5 10 15 His Tyr Ala Ile Gly Gln Phe Asn Ile Asn Gly Leu Gln Trp Thr Lys 20 25 30 Ala Ile Leu Gln Ala Ala Gln Lys Glu Gln Ser Pro Val Ile Ala Ala 35 40 45 Ala Ser Asp Arg Leu Val His Tyr Leu Gly Gly Phe Lys Thr Ile Ala 50 55 60 Ala Met Val Gly Ala Leu Met Glu Asp Met Ala Ile Thr Val Pro Val 65 70 75 80 Val Leu His Leu Asp His Gly Ser Ser Ala Glu Arg Cys Arg Gln Ala 85 90 95 Ile Asp Ala Gly Phe Ser Ser Val Met Ile Asp Gly Ser His Gln Pro 100 105 110 Ile Asp Glu Asn Ile Ala Met Thr Lys Glu Val Thr Asp Tyr Ala Ala 115 120 125 Lys His Gly Val Ser Val Glu Ala Glu Val Gly Thr Val Gly Gly Met 130 135 140 Glu Asp Gly Leu Val Gly Gly Val Arg Tyr Ala Asp Ile Lys Glu Cys 145 150 155 160 Glu Arg Ile Val Lys Glu Thr Asn Ile Asp Ala Leu Ala Ala Ala Leu 165 170 175 Gly Ser Val His Gly Lys Tyr Gln Gly Glu Pro Asn Leu Gly Phe Lys 180 185 190 Glu Met Glu Ala Ile Ser Arg Met Thr His Ile Pro Leu Val Leu His 195 200 205 Gly Ala Ser Gly Ile Pro Gln Asp Gln Ile Lys Lys Ala Ile Thr Leu 210 215 220 Gly His Ala Lys Ile Asn Ile Asn Thr Glu Cys Met Val Ala Trp Thr 225 230 235 240 Asp Glu Thr Arg Arg Met Phe Gln Glu Asn Ser Asp Leu Tyr Glu Pro 245 250 255 Arg Ala Tyr Met Thr Pro Gly Ile Glu Ala Val Glu Glu Thr Val Arg 260 265 270 Ser Lys Met Arg Glu Phe Gly Ser Ala Gly Lys Ala Ala Lys Gln Gln 275 280 285 Val Gly 290 <210> 17 <211> 290 <212> PRT <213> Bacillus subtilis subsp. inaquosorum <400> 17 Met Ala Phe Val Ser Met Lys Glu Leu Leu Glu Asp Ala Lys Arg Glu 1 5 10 15 Gln Tyr Ala Val Gly Gln Phe Asn Ile Asn Gly Leu Gln Trp Thr Lys 20 25 30 Ala Ile Leu Gln Ala Ala Gln Lys Glu Gln Ser Pro Val Ile Ala Ala 35 40 45 Ala Ser Asp Arg Leu Val Asp Tyr Leu Gly Gly Phe Lys Thr Ile Ala 50 55 60 Ala Met Val Gly Ala Leu Met Glu Asp Met Ala Ile Thr Val Pro Val 65 70 75 80 Val Leu His Leu Asp His Gly Ser Ser Ala Glu Arg Cys Arg Gln Ala 85 90 95 Ile Asp Ala Gly Phe Ser Ser Val Met Ile Asp Gly Ser His Gln Pro 100 105 110 Ile Asp Glu Asn Ile Val Met Thr Lys Glu Val Thr Asp Tyr Ala Gly 115 120 125 Lys His Gly Val Ser Val Glu Ala Glu Val Gly Thr Val Gly Gly Met 130 135 140 Glu Asp Gly Leu Val Gly Gly Val Arg Tyr Ala Asp Ile Thr Glu Cys 145 150 155 160 Glu Arg Ile Val Lys Glu Thr Asn Ile Asp Ala Leu Ala Ala Ala Leu 165 170 175 Gly Ser Val His Gly Lys Tyr Gln Gly Glu Pro Asn Leu Gly Phe Lys 180 185 190 Glu Met Glu Ala Ile Ser Arg Met Thr Asp Ile Pro Leu Val Leu His 195 200 205 Gly Ala Ser Gly Ile Pro Gln Asp Gln Ile Lys Lys Ala Ile Thr Leu 210 215 220 Gly His Ala Lys Ile Asn Ile Asn Thr Glu Cys Met Val Ala Trp Thr 225 230 235 240 Asp Glu Thr Arg Arg Met Phe Gln Glu Asn Ser Asp Leu Tyr Glu Pro 245 250 255 Arg Gly Tyr Met Thr Pro Gly Ile Glu Ala Val Glu Glu Thr Val Arg 260 265 270 Ser Lys Met Arg Glu Phe Gly Ser Ala Gly Lys Ala Ala Lys Gln Gln 275 280 285 Val Gly 290 <210> 18 <211> 285 <212> PRT <213> Bacillus weihenstephanensis <400> 18 Met Pro Leu Val Ser Met Lys Glu Met Leu Asn Thr Ala Leu Glu Gly 1 5 10 15 Lys Tyr Ala Val Gly Gln Phe Asn Met Asn Asn Leu Glu Trp Thr Gln 20 25 30 Ala Ile Leu Ala Ala Ala Glu Glu Glu Lys Ser Pro Val Ile Leu Gly 35 40 45 Val Ser Glu Gly Ala Ala Arg His Met Thr Gly Phe Lys Thr Val Val 50 55 60 Ala Met Val Lys Ala Leu Ile Glu Glu Met Asn Ile Ser Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Lys Cys Lys Glu Ala 85 90 95 Ile Asp Ala Gly Phe Thr Ser Val Met Ile Asp Ala Ser His His Pro 100 105 110 Phe Asp Glu Asn Val Glu Thr Thr Lys Lys Val Val Glu Tyr Ala His 115 120 125 Ala Arg Asn Val Ser Val Glu Ala Glu Leu Gly Thr Val Gly Gly Gln 130 135 140 Glu Asp Asp Ile Ile Ala Glu Gly Val Ile Tyr Ala Asp Pro Ala Glu 145 150 155 160 Cys Lys His Leu Val Glu Ala Thr Gly Ile Asp Cys Leu Ala Pro Ala 165 170 175 Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Asn Leu Gly Phe 180 185 190 Ala Glu Met Glu Gln Val Arg Asp Phe Thr Gly Val Pro Leu Val Leu 195 200 205 His Gly Gly Thr Gly Ile Pro Thr Ala Asp Ile Val Lys Ala Ile Ser 210 215 220 Leu Gly Thr Ser Lys Ile Asn Val Asn Thr Glu Asn Gln Ile Glu Phe 225 230 235 240 Thr Lys Ala Val Arg Glu Ala Leu Asn Lys Asp Gln Glu Val Tyr Asp 245 250 255 Pro Arg Lys Tyr Ile Gly Pro Gly Arg Asp Ala Ile Lys Ala Thr Val 260 265 270 Ala Gly Lys Met Arg Glu Phe Gly Ser Asn Gly Lys Ala 275 280 285 <210> 19 <211> 355 <212> PRT <213> Bifidobacterium breve <400> 19 Met Thr Ile Ala Thr Pro Glu Arg Tyr Ala Glu Met Leu Asp Ala Ala 1 5 10 15 Arg Arg Gly Gly Tyr Ala Tyr Pro Ala Ile Asn Val Ser Ser Ser Gln 20 25 30 Thr Leu Asn Ala Ala Leu Lys Gly Phe Ala Asp Ala Glu Ser Asp Gly 35 40 45 Ile Ile Gln Val Ser Val Gly Ala Ala Ala Tyr Leu Ser Gly Ile Gly 50 55 60 Val Asn Asp Arg Val Thr Gly Ser Leu Ala Leu Ala Ala Phe Ala His 65 70 75 80 Glu Val Ala Ala Lys Tyr Pro Asn Ile Thr Ile Ala Leu His Thr Asp 85 90 95 His Cys Ala Lys Gln Tyr Leu Asp Glu Trp Val Arg Pro Leu Leu Ala 100 105 110 His Glu Ala Asp Gln Val Ala His Gly Gln Glu Pro Thr Phe Gln Ser 115 120 125 His Met Trp Asp Gly Ser Thr Val Pro Leu Lys Glu Asn Leu Asp Ile 130 135 140 Ala Glu Glu Leu Leu Asp Lys Ser Gln Ala Ala His Thr Val Leu Glu 145 150 155 160 Ile Glu Ile Gly Ala Val Gly Gly Glu Glu Asp Gly Tyr Ser Ala Lys 165 170 175 Ile Asp Glu Arg Leu Tyr Ser Thr Pro Ala Asp Gly Leu Glu Val Ala 180 185 190 Arg Arg Leu Gly Leu Gly Glu Arg Gly Arg Tyr Met Ala Ala Phe Thr 195 200 205 Phe Gly Asn Val His Gly Ser Tyr Lys Pro Gly Val Val Lys Leu Arg 210 215 220 Pro Ala Leu Leu Gly Asp Ile Gln Ser Arg Val Ala Arg Ala Val Val 225 230 235 240 Glu Gly Glu Leu Pro Ser Ala Ala Gly Ile Val Asp Phe Pro Asn Gly 245 250 255 Lys Pro Phe Glu Leu Val Phe His Gly Gly Ser Gly Ser Arg Pro Glu 260 265 270 Glu Ile Ala Glu Ala Val Ser His Gly Val Ile Lys Met Asn Ile Asp 275 280 285 Thr Asp Thr Gln Tyr Ser Phe Thr Arg Pro Ile Ala Asp His Val Phe 290 295 300 Glu Asn Tyr Asp Lys Val Leu Lys Ile Asp Gly Glu Val Gly Glu Lys 305 310 315 320 Lys Phe Tyr Asp Pro Arg Ser Trp Gly Arg Lys Ala Glu Glu Ser Met 325 330 335 Ser Ala Arg Val Ala Glu Ala Cys Lys Gln Leu Gly Ser Ala Gly Lys 340 345 350 Ala Leu Arg 355 <210> 20 <211> 344 <212> PRT <213> Corynebacterium glutamicum <400> 20 Met Pro Ile Ala Thr Pro Glu Val Tyr Asn Glu Met Leu Asp Arg Ala 1 5 10 15 Lys Glu Gly Gly Phe Ala Phe Pro Ala Ile Asn Cys Thr Ser Ser Glu 20 25 30 Thr Ile Asn Ala Ala Leu Lys Gly Phe Ala Glu Ala Glu Ser Asp Gly 35 40 45 Ile Ile Gln Phe Ser Thr Gly Gly Ala Glu Phe Gly Ser Gly Leu Ala 50 55 60 Val Lys Asn Lys Val Lys Gly Ala Val Ala Leu Ala Ala Phe Ala His 65 70 75 80 Glu Ala Ala Lys Ser Tyr Gly Ile Asn Val Ala Leu His Thr Asp His 85 90 95 Cys Gln Lys Glu Val Leu Asp Glu Tyr Val Arg Pro Leu Leu Ala Ile 100 105 110 Ser Gln Glu Arg Val Asp Arg Gly Glu Leu Pro Leu Phe Gln Ser His 115 120 125 Met Trp Asp Gly Ser Ala Val Pro Ile Asp Glu Asn Leu Glu Ile Ala 130 135 140 Gln Glu Leu Leu Ala Lys Ala Lys Ala Ala Asn Ile Ile Leu Glu Val 145 150 155 160 Glu Ile Gly Val Val Gly Gly Glu Glu Asp Gly Val Glu Ala Lys Ala 165 170 175 Gly Ala Asn Leu Tyr Thr Ser Pro Glu Asp Phe Glu Lys Thr Ile Asp 180 185 190 Ala Ile Gly Thr Gly Glu Lys Gly Arg Tyr Leu Leu Ala Ala Thr Phe 195 200 205 Gly Asn Val His Gly Val Tyr Lys Pro Gly Asn Val Lys Leu Arg Pro 210 215 220 Glu Val Leu Leu Glu Gly Gln Gln Val Ala Arg Lys Lys Leu Gly Leu 225 230 235 240 Ala Asp Asp Ala Leu Pro Phe Asp Phe Val Phe His Gly Gly Ser Gly 245 250 255 Ser Glu Lys Glu Lys Ile Glu Glu Ala Leu Thr Tyr Gly Val Ile Lys 260 265 270 Met Asn Val Asp Thr Asp Thr Gln Tyr Ala Phe Thr Arg Pro Ile Val 275 280 285 Ser His Met Phe Glu Asn Tyr Asn Gly Val Leu Lys Ile Asp Gly Glu 290 295 300 Val Gly Asn Lys Lys Ala Tyr Asp Pro Arg Ser Tyr Met Lys Lys Ala 305 310 315 320 Glu Gln Ser Met Ser Glu Arg Ile Ile Glu Ser Cys Gln Asp Leu Lys 325 330 335 Ser Val Gly Lys Thr Thr Ser Lys 340 <210> 21 <211> 296 <212> PRT <213> Corynebacterium kroppenstedtii <400> 21 Met Asn Gln Glu Gln Ala Thr Arg Met Thr Glu Ala Lys Gly Phe Ile 1 5 10 15 Ala Ala Leu Asp Gln Ser Gly Gly Ser Thr Pro Lys Ala Leu Arg Ala 20 25 30 Tyr Gly Ile Gln Asp Asp Ala Tyr Ser Ser Asp Ala Glu Met Phe Asp 35 40 45 Leu Val His Ala Met Arg Ser Arg Leu Ile Thr Ser Pro Ser Phe Thr 50 55 60 Ser Glu Lys Val Leu Ala Ala Ile Leu Phe Glu Met Thr Met Asp Arg 65 70 75 80 Glu Ile Glu Gly Ile Pro Thr Ala Gln Tyr Leu Trp Glu Lys Lys Gly 85 90 95 Ile Val Pro Leu Leu Lys Ile Asp Lys Gly Leu Ala Asp Glu Glu Asn 100 105 110 Asp Val Gln Val Met Lys Pro Met Pro Glu Leu Asp Lys Leu Leu Asp 115 120 125 Arg Gly Val Glu Lys Gly Ile Phe Gly Thr Lys Glu Arg Ser Val Ile 130 135 140 Asn Ala Ala Asn Gly Glu Gly Ile Gln Ala Val Val Asp Gln Gln Phe 145 150 155 160 Glu Ile Gly Lys Gln Val Leu Ala Lys Gly Met Val Pro Ile Leu Glu 165 170 175 Pro Glu Val Thr Ile Ser Ile Asp Asp Lys Glu Glu Ala Glu Glu Leu 180 185 190 Leu Lys Ala Ala Leu Leu Lys Gly Leu Asp Asp Ile Pro Glu Gly Gln 195 200 205 Gln Val Met Ile Lys Val Ser Ile Pro Thr Val Asp Asn Phe Tyr Glu 210 215 220 Asp Leu Ile Asn His Pro Lys Val Met Arg Val Val Ala Leu Ser Gly 225 230 235 240 Gly Tyr Glu Arg Asp Asp Ala Asn Glu Arg Leu Ser Arg Asn Lys Gly 245 250 255 Leu Ile Ala Ser Phe Ser Arg Ala Leu Leu Glu Gly Leu Thr Ala Asp 260 265 270 Gln Ser Gln Glu Glu Phe Asp Asn Thr Leu Ala Thr Thr Ile Asp Gly 275 280 285 Ile Tyr Lys Ala Ser Ile Ala Gly 290 295 <210> 22 <211> 289 <212> PRT <213> Enterococcus asini <400> 22 Met Pro Val Val Ser Ser Val Glu Phe Leu Lys Ala Ala Arg Lys Gly 1 5 10 15 Gly Tyr Ala Ile Gly Gly Tyr Asn Thr Asn Asn Leu Glu Trp Thr Gln 20 25 30 Ala Leu Leu Thr Ala Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln 35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Val Ile Val 50 55 60 Asp Leu Val Asn Asn Leu Ile Asp Ser Met Gly Ile Thr Val Pro Val 65 70 75 80 Ala Leu His Leu Asp His Gly Asp Tyr Glu Ala Ala Leu Glu Cys Ile 85 90 95 Glu Val Gly Tyr Ser Ser Val Met Phe Asp Gly Ser His Leu Pro Phe 100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Asp Val Val Glu Lys Ala His Ala 115 120 125 Lys Gly Ile Ala Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu 130 135 140 Asp Gly Ile Ile Gly Ala Gly Glu Leu Ala Asp Ile Asn Glu Cys Val 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Ser Leu Ala Cys Gly Ile Gly Asn 165 170 175 Ile His Gly Val Tyr Pro Glu Asn Trp Gln Gly Leu Ala Phe Asp His 180 185 190 Leu Gln Glu Ile Ala Ala Ala Val Gly Glu Asp Val Pro Leu Val Leu 195 200 205 His Gly Gly Ser Gly Ile Pro Gln Glu Gln Ile Glu Lys Ala Ile Ser 210 215 220 Met Gly Ile Ser Lys Val Asn Val Asn Thr Glu Phe Gln Leu Ser Phe 225 230 235 240 Ala Lys Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp His Glu Gly 245 250 255 Lys Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Lys Ala Ala Ile 260 265 270 Ile Lys Asp Ala Glu Glu His Ile Asp Trp Phe Gly Ser Ala Asn Lys 275 280 285 Ala <210> 23 <211> 288 <212> PRT <213> Enterococcus caccae <400> 23 Met Pro Leu Val Ser Gly Thr Glu Phe Leu Lys Ala Ala Arg Lys Gly 1 5 10 15 Gly Tyr Gly Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Lys 20 25 30 Ala Ile Leu Glu Ala Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln 35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Gln Val Cys Tyr 50 55 60 Asp Leu Val Lys Asp Leu Ile Asp Ser Met Gly Ile Thr Val Pro Val 65 70 75 80 Ala Leu His Leu Asp His Gly Glu Tyr Glu Asp Ala Leu Glu Cys Ile 85 90 95 Glu Ile Gly Tyr Thr Ser Val Met Phe Asp Gly Ser His Leu Pro Phe 100 105 110 Glu Glu Asn Ile Glu Lys Ala Lys Asp Val Val Ala Lys Ala His Ala 115 120 125 Lys Gly Val Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu 130 135 140 Asp Gly Ile Ile Gly Ser Gly Glu Leu Ala Asp Val Gln Glu Cys Val 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn 165 170 175 Ile His Gly Ser Tyr Pro Asp Asn Trp Thr Gly Leu Ala Phe Asp His 180 185 190 Leu Gln Glu Ile Ala Asp Ala Val Gly Asp Ile Pro Leu Val Leu His 195 200 205 Gly Gly Ser Gly Ile Pro Leu Glu Gln Val Gln Lys Ala Ile Ser Met 210 215 220 Gly Val Ser Lys Ile Asn Val Asn Thr Glu Cys Gln Glu Val Phe Ala 225 230 235 240 Ala Ala Thr Arg Lys Tyr Ile Glu Glu Gly Lys Asp Leu Glu Gly Lys 245 250 255 Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Thr Lys Ala Val Thr 260 265 270 Glu Leu Val Glu Gln Arg Ile Glu Trp Phe Gly Ser Ala Asn Lys Ala 275 280 285 <210> 24 <211> 304 <212> PRT <213> Enterococcus casseliflavus <400> 24 Met Glu His Thr Tyr Met Leu Ile Asn Met Lys Gln Leu Leu Thr Val 1 5 10 15 Ala Lys Glu Asn Gln Phe Ala Val Gly Ala Phe Asn Val Ala Asp Ser 20 25 30 Asn Phe Leu Arg Val Val Ile Glu Ala Ala Glu Glu Asn Asp Ala Pro 35 40 45 Ala Ile Ile Ala Ile His Pro Thr Glu Leu Ala Phe Thr Lys Asp Glu 50 55 60 Phe Phe His Tyr Ala Val Ser Arg Ala Lys Asn Ser Ser Val Pro Ile 65 70 75 80 Val Ile His Met Asp His Gly Asp Ser Leu Ala Ser Ile Met Arg Ala 85 90 95 Ile His Cys Gly Phe Ser Ser Val Met Ile Asp Gly Ser Leu Leu Pro 100 105 110 Phe Glu Glu Asn Ile Ala Leu Thr Lys Gln Ile Thr Glu Ile Cys His 115 120 125 Pro Ile Gly Val Ser Val Glu Gly Glu Leu Gly Thr Ile Gly Asn Thr 130 135 140 Gly Thr Ser Val Glu Gly Gly Val Ser Glu Val Ile Tyr Thr Arg Pro 145 150 155 160 Glu Asp Ala Gln Glu Phe Ile Glu Lys Thr Gly Ile Asp Thr Leu Ala 165 170 175 Val Ala Ile Gly Thr Ala His Gly Ile Tyr Pro Lys Asp Ile Lys Pro 180 185 190 Glu Leu Lys Met Asp Val Leu Gln Glu Ile Lys Asp Arg Val Asp Ile 195 200 205 Pro Leu Val Leu His Gly Gly Ser Ala Asn Pro Asp Glu Glu Ile Ala 210 215 220 Gln Ala Val Ala Ile Gly Ile Gln Lys Val Asn Ile Ser Ser Asp Tyr 225 230 235 240 Lys Tyr Ala Phe Tyr Lys Lys Cys Arg Gln Ile Leu Ala Glu Thr Glu 245 250 255 Leu Trp Asp Pro Asn Ala Ile Tyr Pro Glu Cys Ile Glu Ala Ala Lys 260 265 270 Ala Val Val Thr Gln Lys Met Thr Leu Phe Asn Ala Ile Gly Lys Ala 275 280 285 Ala Leu Tyr Arg Gln Gln Asn Ile Gln Pro Trp Arg Ser Glu Gln Asn 290 295 300 <210> 25 <211> 289 <212> PRT <213> Enterococcus faecalis <400> 25 Met Pro Val Val Ser Gly Ala Glu Phe Leu Lys Ala Ala Arg Lys Gly 1 5 10 15 Gly Tyr Ala Val Gly Gly Tyr Asn Thr Asn Asn Leu Glu Trp Thr Gln 20 25 30 Ala Ile Leu Glu Ala Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln 35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Val Ala Lys 50 55 60 Asp Met Ile Thr Asn Leu Val Asp Ser Met Asn Ile Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Asp Tyr Glu Ala Ala Leu Glu Cys Ile 85 90 95 Glu Val Gly Tyr Thr Ser Ile Met Phe Asp Gly Ser His Leu Pro Phe 100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Asp Val Val Glu Lys Ala His Ala 115 120 125 Lys Gly Ile Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu 130 135 140 Asp Gly Ile Ile Gly Thr Gly Glu Leu Ala Asp Ile Glu Glu Cys Lys 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn 165 170 175 Ile His Gly Gln Tyr Pro Glu Asn Trp Lys Gly Leu Ala Phe Asp His 180 185 190 Leu Gln Ala Ile Ala Glu Ala Val Gly Ser Asp Val Pro Leu Val Leu 195 200 205 His Gly Gly Ser Gly Ile Pro Gln Glu Gln Ile Glu Lys Ala Ile Ser 210 215 220 Met Gly Ile Ser Lys Val Asn Val Asn Thr Glu Phe Gln Leu Ser Phe 225 230 235 240 Ala Lys Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Leu Glu Gly 245 250 255 Lys Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Lys Ala Ala Ile 260 265 270 Ile Lys Asp Ala Glu Glu His Ile Asp Trp Phe Gly Ser Ala Asn Lys 275 280 285 Ala <210> 26 <211> 289 <212> PRT <213> Enterococcus faecium <400> 26 Met Tyr Thr Thr Leu Lys Glu Val Thr Lys Thr Ala Glu Glu Leu Asn 1 5 10 15 Phe Thr Val Gly Ala Phe Asn Thr His Asn Leu Glu Met Leu Pro Ala 20 25 30 Met Leu Gly Ala Ala Lys Glu Met Gly Ser Pro Ile Ile Ile Gln Thr 35 40 45 Ser Val Asp Thr Ala Lys Tyr Ile Gly Met His Val Leu Val Asn Val 50 55 60 Met Thr Ala Ile Ala Glu Glu Glu Met Val Asp Ala Val Leu His Leu 65 70 75 80 Asp His Ala Arg Asp Phe Asp Asp Ile Lys Glu Ala Ile Asp Ser Gly 85 90 95 Tyr Thr Ser Val Met Tyr Asp Gly Ser His Leu Pro Phe Lys Glu Asn 100 105 110 Ile Leu Lys Thr Lys Ala Val Val Glu Tyr Ala His Ala His Gly Val 115 120 125 Ser Val Glu Gly Glu Leu Gly Thr Ile Gly Gly Thr Glu Glu Gly Ile 130 135 140 His Val Ala Glu Asn Asp Lys Val Tyr Thr Arg Pro Glu Asp Ala Val 145 150 155 160 Lys Phe Val Glu Ala Thr Gly Val Asp Ala Leu Ala Ile Ala Ile Gly 165 170 175 Thr Asn His Gly Gln Phe Lys Ser Lys Thr Glu Val Asn Ile Pro Leu 180 185 190 Leu Lys Glu Ile Asp Ala Val Val Asp Val Pro Leu Val Ile His Gly 195 200 205 Gly Thr Gly Val Lys Glu Glu Asp Tyr Pro Glu Leu Ile Asn Asn Gly 210 215 220 Ile Arg Lys Phe Asn Val Gly Thr Glu Leu Leu Val Asn Trp Thr Lys 225 230 235 240 Val Ala Lys Glu Thr Phe Ser Glu Thr Glu Ile Asn Lys Ser Leu Arg 245 250 255 His Asn Val Ile Pro Ala Asn Glu Ala Val Lys Glu Ile Val Lys His 260 265 270 Lys Ile Gly Leu Phe Met Asn Leu Glu Ser Pro Ile Asn Leu Gly Asn 275 280 285 Lys <210> 27 <211> 289 <212> PRT <213> Enterococcus flavescens <400> 27 Met Pro Val Val Ser Gly Ala Glu Phe Leu Lys Ala Ala Arg Gln Gly 1 5 10 15 Gly Tyr Ala Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Gln 20 25 30 Ala Ile Leu Lys Ala Ala Glu Ala Lys Lys Ala Pro Val Leu Leu Gln 35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Val Ala Lys 50 55 60 Asp Ile Val Thr Asn Leu Val Asp Ser Met Asn Ile Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Asp Tyr Asp Ala Ala Met Glu Cys Ile 85 90 95 Glu Val Gly Tyr Thr Ser Val Met Phe Asp Gly Ser His Leu Pro Phe 100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Glu Val Val Glu Lys Ala His Ala 115 120 125 Lys Gly Val Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu 130 135 140 Asp Gly Ile Ile Gly Ser Gly Glu Leu Ala Asp Ile Glu Glu Cys Lys 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn 165 170 175 Ile His Gly Gln Tyr Pro Glu Asn Trp Lys Gly Leu Ala Phe Asp His 180 185 190 Leu Gln Ala Ile Ala Asp Ala Val Gly Ser Asp Val Pro Leu Val Leu 195 200 205 His Gly Gly Ser Gly Ile Pro Gln Asp Gln Ile Glu Lys Ala Ile Ser 210 215 220 Met Gly Val Ser Lys Val Asn Val Asn Thr Glu Phe Gln Leu Ser Phe 225 230 235 240 Ala Lys Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Lys Glu Gly 245 250 255 Lys Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Val Ala Ala Ile 260 265 270 Val Lys Asp Ala Glu Glu His Ile Asp Trp Phe Gly Ser Ala Gly Lys 275 280 285 Ala <210> 28 <211> 289 <212> PRT <213> Enterococcus gilvus <400> 28 Met Pro Val Val Ser Gly Ala Glu Phe Leu Lys Ala Ala Arg Lys Gly 1 5 10 15 Gly Tyr Ala Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Gln 20 25 30 Ala Ile Leu Lys Ala Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln 35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Val Ala Lys 50 55 60 Asp Ile Ile Cys Asn Leu Val Asp Ser Met Gly Ile Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Asp Tyr Glu Ala Ala Leu Glu Cys Ile 85 90 95 Glu Val Gly Tyr Thr Ser Ile Met Phe Asp Gly Ser His Leu Pro Phe 100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Asp Val Val Glu Lys Ala His Ala 115 120 125 Lys Gly Ile Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu 130 135 140 Asp Gly Ile Ile Gly Ala Gly Glu Leu Ala Asp Val Asp Glu Cys Val 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn 165 170 175 Ile His Gly Val Tyr Pro Glu Asn Trp Lys Gly Leu Ala Phe Asp His 180 185 190 Leu Gln Glu Ile Ala Asn Ala Val Gly Ser Asp Val Pro Leu Val Leu 195 200 205 His Gly Gly Ser Gly Ile Pro Gln Glu Gln Ile Gln Lys Ala Ile Ser 210 215 220 Met Gly Val Ser Lys Val Asn Val Asn Thr Glu Phe Gln Leu Ser Phe 225 230 235 240 Ala Lys Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Lys Glu Gly 245 250 255 Lys Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Val Ala Ala Ile 260 265 270 Val Lys Asp Ala Glu Glu His Ile Asp Trp Phe Gly Ser Ala Asn Lys 275 280 285 Ala <210> 29 <211> 288 <212> PRT <213> Enterococcus haemoperoxidus <400> 29 Met Pro Leu Val Ser Gly Thr Glu Phe Leu Lys Ala Ala Arg Lys Gly 1 5 10 15 Gly Tyr Gly Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Lys 20 25 30 Ala Ile Leu Glu Ala Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln 35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Gln Val Cys Tyr 50 55 60 Asp Leu Val Lys Asp Leu Ile Asp Ser Met Gly Ile Thr Val Pro Val 65 70 75 80 Ala Leu His Leu Asp His Gly Glu Tyr Glu Asp Ala Leu Glu Cys Ile 85 90 95 Glu Ile Gly Tyr Thr Ser Val Met Phe Asp Gly Ser His Leu Pro Phe 100 105 110 Glu Glu Asn Ile Glu Lys Ala Lys Asp Val Val Ala Lys Ala His Ala 115 120 125 Lys Gly Val Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu 130 135 140 Asp Gly Ile Ile Gly Ser Gly Glu Leu Ala Asp Val Gln Glu Cys Val 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn 165 170 175 Ile His Gly Ser Tyr Pro Asp Asn Trp Thr Gly Leu Ala Phe Asp His 180 185 190 Leu Gln Glu Ile Ala Asp Ala Val Gly Asp Ile Pro Leu Val Leu His 195 200 205 Gly Gly Ser Gly Ile Pro Leu Glu Gln Val Gln Lys Ala Ile Ser Met 210 215 220 Gly Val Ser Lys Ile Asn Val Asn Thr Glu Cys Gln Glu Val Phe Ala 225 230 235 240 Ala Ala Thr Arg Lys Tyr Ile Glu Glu Gly Lys Asp Leu Glu Gly Lys 245 250 255 Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Thr Lys Ala Val Thr 260 265 270 Glu Leu Val Glu Gln Arg Ile Glu Trp Phe Gly Ser Ala Asn Lys Ala 275 280 285 <210> 30 <211> 290 <212> PRT <213> Enterococcus italicus <400> 30 Met Pro Leu Val Ser Gly Thr Glu Ile Phe Lys Ala Ala Arg Lys Gly 1 5 10 15 Gly Tyr Ala Val Gly Ala Phe Asn Thr Asn Asn Leu Glu Trp Thr Gln 20 25 30 Ala Ile Leu Glu Ala Ala Glu Ala Lys Gln Thr Pro Val Leu Ile Gln 35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Leu Val Lys 50 55 60 Asp Met Val Thr Asp Leu Ile Glu Ser Leu Asn Ile Thr Val Pro Val 65 70 75 80 Val Leu His Leu Asp His Gly His Tyr Glu Asp Ala Leu Glu Cys Ile 85 90 95 Glu Val Gly Tyr Thr Ser Val Met Phe Asp Gly Ser His Leu Pro Phe 100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Glu Val Val Ala Lys Ala His Ala 115 120 125 Lys Gly Val Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu 130 135 140 Asp Gly Ile Ile Gly Ala Gly Glu Leu Ala Asp Val Asp Glu Cys Val 145 150 155 160 Gln Met Val Asn Thr Gly Ile Asp Phe Leu Ala Cys Gly Ile Gly Asn 165 170 175 Ile His Gly Lys Tyr Pro Glu Asn Trp Lys Gly Leu Ala Phe Asp His 180 185 190 Leu Gln Ala Ile Ala Asp Ala Val Gly Ser Asp Val Pro Leu Val Leu 195 200 205 His Gly Gly Ser Gly Ile Pro Gln Asp Gln Ile Glu Lys Ala Ile Ser 210 215 220 Met Gly Ile Ser Lys Val Asn Val Asn Thr Glu Phe Gln Leu Ser Phe 225 230 235 240 Ala Lys Ala Ile Arg Glu Tyr Ile Glu Ala Gly Lys Asp Leu Glu Gly 245 250 255 Lys Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Lys Ala Ala Ile 260 265 270 Ile Lys Asp Cys Glu Glu His Ile Asp Trp Phe Gly Ser Ala Asn Lys 275 280 285 Ala Lys 290 <210> 31 <211> 288 <212> PRT <213> Enterococcus moraviensis <400> 31 Met Pro Leu Val Ser Gly Thr Glu Phe Leu Lys Ala Ala Arg Lys Gly 1 5 10 15 Gly Tyr Gly Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Lys 20 25 30 Ala Ile Leu Glu Ala Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln 35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Gln Val Cys Tyr 50 55 60 Asp Leu Val Lys Asp Leu Ile Asp Ser Met Gly Ile Thr Val Pro Val 65 70 75 80 Ala Leu His Leu Asp His Gly Glu Tyr Glu Asp Ala Leu Glu Cys Ile 85 90 95 Glu Ile Gly Tyr Thr Ser Val Met Phe Asp Gly Ser His Leu Pro Phe 100 105 110 Glu Glu Asn Ile Glu Lys Ala Lys Asp Val Val Ala Lys Ala His Ala 115 120 125 Lys Gly Val Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu 130 135 140 Asp Gly Ile Ile Gly Ser Gly Glu Leu Ala Asp Val Gln Glu Cys Val 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn 165 170 175 Ile His Gly Ser Tyr Pro Glu Asn Trp Thr Gly Leu Ala Phe Asp His 180 185 190 Leu Gln Glu Ile Ala Asp Ala Val Gly Asp Ile Pro Leu Val Leu His 195 200 205 Gly Gly Ser Gly Ile Pro Leu Glu Gln Val Gln Lys Ala Ile Ser Met 210 215 220 Gly Val Ser Lys Ile Asn Val Asn Thr Glu Cys Gln Glu Val Phe Ala 225 230 235 240 Ala Ala Thr Arg Lys Tyr Ile Glu Glu Gly Lys Asp Leu Glu Gly Lys 245 250 255 Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Thr Lys Ala Val Thr 260 265 270 Glu Leu Val Glu Gln Arg Ile Glu Trp Phe Gly Ser Ala Asn Lys Ala 275 280 285 <210> 32 <211> 289 <212> PRT <213> Enterococcus mundtii <400> 32 Met Pro Val Val Ser Gly Ala Glu Phe Leu Gln Ala Ala Arg Lys Gly 1 5 10 15 Gly Tyr Ala Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Gln 20 25 30 Ala Ile Leu Glu Ala Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln 35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Val Cys Lys 50 55 60 Asp Met Ile Thr Asn Leu Val Glu Ser Met Asn Ile Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Asp Tyr Asp Ala Ala Leu Glu Cys Ile 85 90 95 Glu Val Gly Tyr Thr Ser Val Met Phe Asp Gly Ser His Leu Pro Phe 100 105 110 Glu Glu Asn Leu Lys Leu Ala Arg Asp Val Val Glu Lys Ala His Ala 115 120 125 Lys Gly Ile Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu 130 135 140 Asp Gly Ile Ile Gly Thr Gly Glu Leu Ala Asp Ile Glu Glu Cys Lys 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn 165 170 175 Ile His Gly Gln Tyr Pro Glu Asn Trp Ser Gly Leu Ala Phe Asp His 180 185 190 Leu Gln Ala Ile Ala Asp Ala Val Gly Ser Asp Val Pro Leu Val Leu 195 200 205 His Gly Gly Ser Gly Ile Pro Gln Asp Gln Ile Glu Lys Ala Ile Ser 210 215 220 Met Gly Ile Ser Lys Val Asn Val Asn Thr Glu Phe Gln Leu Ser Phe 225 230 235 240 Ala Ala Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Arg Glu Gly 245 250 255 Lys Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Lys Ala Ala Ile 260 265 270 Val Lys Asp Ala Glu Ser His Ile Asp Trp Phe Gly Ser Ala Asn Lys 275 280 285 Ala <210> 33 <211> 289 <212> PRT <213> Enterococcus pallens <400> 33 Met Pro Val Val Ser Gly Ala Glu Phe Leu Lys Ala Ala Arg Gln Gly 1 5 10 15 Gly Tyr Ala Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Gln 20 25 30 Ala Ile Leu Arg Ala Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln 35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Val Ala Lys 50 55 60 Asp Ile Ile Cys Asp Leu Val Glu Ser Met Asp Ile Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Ser Tyr Glu Ala Ala Leu Glu Cys Ile 85 90 95 Glu Val Gly Tyr Thr Ser Val Met Phe Asp Gly Ser His Leu Pro Phe 100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Asp Val Val Glu Lys Ala His Ala 115 120 125 Lys Gly Val Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu 130 135 140 Asp Gly Ile Ile Gly Ala Gly Glu Leu Ala Asp Ile Asp Glu Cys Val 145 150 155 160 Gln Met Val Gly Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn 165 170 175 Ile His Gly Val Tyr Pro Glu Asn Trp Lys Gly Leu Ala Phe Asp His 180 185 190 Leu Gln Ala Ile Ala Asp Ala Val Gly Ser Glu Val Pro Leu Val Leu 195 200 205 His Gly Gly Ser Gly Ile Pro Gln Glu Gln Ile Gln Lys Ala Ile Ser 210 215 220 Met Gly Val Ser Lys Val Asn Val Asn Thr Glu Phe Gln Leu Ser Phe 225 230 235 240 Ala Lys Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Lys Glu Gly 245 250 255 Lys Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Val Ala Ala Ile 260 265 270 Val Lys Asp Ala Glu Glu His Ile Asp Trp Phe Gly Ser Ala Asn Lys 275 280 285 Ala <210> 34 <211> 288 <212> PRT <213> Enterococcus phoeniculicola <400> 34 Met Pro Leu Val Ser Gly Thr Glu Phe Leu Lys Ala Ala His Lys Gly 1 5 10 15 Gly Tyr Gly Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Lys 20 25 30 Ala Ile Leu Glu Ala Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln 35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Val Cys Tyr 50 55 60 Asp Leu Val Lys Asp Leu Val Asp Ser Met Gly Ile Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Glu Tyr Glu Asp Ala Leu Glu Cys Ile 85 90 95 Glu Ile Gly Tyr Thr Ser Val Met Phe Asp Gly Ser His Leu Pro Phe 100 105 110 Glu Glu Asn Ile Glu Lys Ala Lys Asp Val Val Ala Lys Ala His Ala 115 120 125 Lys Gly Val Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu 130 135 140 Asp Gly Ile Ile Gly Ser Gly Glu Leu Ala Asp Ile Glu Glu Cys Lys 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn 165 170 175 Ile His Gly Ser Tyr Pro Asp Asn Trp Thr Gly Leu Ala Phe Asp His 180 185 190 Leu Gln Ala Ile Ser Asp Ala Val Gly Asp Ile Pro Leu Val Leu His 195 200 205 Gly Gly Ser Gly Ile Pro Leu Glu Gln Val Gln Lys Ala Ile Ser Met 210 215 220 Gly Val Ser Lys Ile Asn Val Asn Thr Glu Cys Gln Glu Val Phe Ala 225 230 235 240 Ala Ala Thr Arg Lys Tyr Ile Glu Glu Gly Lys Asp Leu Glu Gly Lys 245 250 255 Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Thr Lys Ala Val Thr 260 265 270 Glu Leu Val Glu Gln Arg Ile Glu Trp Phe Gly Ser Thr Asn Lys Ala 275 280 285 <210> 35 <211> 289 <212> PRT <213> Enterococcus raffinosus <400> 35 Met Pro Val Val Ser Gly Ala Glu Phe Leu Lys Ala Ala Arg Lys Gly 1 5 10 15 Gly Tyr Ala Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Gln 20 25 30 Ala Ile Leu Lys Ala Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln 35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Val Ala Lys 50 55 60 Asp Ile Ile Thr Asp Leu Val Asp Ser Met Gly Ile Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Asp Tyr Glu Ala Ala Leu Glu Cys Ile 85 90 95 Glu Val Gly Tyr Thr Ser Ile Met Phe Asp Gly Ser His Leu Pro Phe 100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Asp Val Val Glu Lys Ala His Ala 115 120 125 Lys Gly Ile Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu 130 135 140 Asp Gly Ile Ile Gly Ala Gly Glu Leu Ala Asp Ile Asp Glu Cys Val 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn 165 170 175 Ile His Gly Val Tyr Pro Glu Asn Trp Lys Gly Leu Ala Phe Asp His 180 185 190 Leu Gln Ala Ile Ala Asp Ala Val Gly Asn Asp Val Pro Leu Val Leu 195 200 205 His Gly Gly Ser Gly Ile Pro Gln Glu Gln Ile Gln Lys Ala Ile Ser 210 215 220 Met Gly Val Ser Lys Val Asn Val Asn Thr Glu Phe Gln Leu Ser Phe 225 230 235 240 Ala Lys Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Lys Glu Gly 245 250 255 Lys Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Val Ala Ala Ile 260 265 270 Val Lys Asp Ala Glu Glu His Ile Asp Trp Phe Gly Ser Ala Asn Lys 275 280 285 Ala <210> 36 <211> 289 <212> PRT <213> Enterococcus saccharolyticus <400> 36 Met Pro Val Val Ser Gly Ala Glu Phe Leu Lys Ala Ala Arg Gln Gly 1 5 10 15 Gly Tyr Ala Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Gln 20 25 30 Ala Ile Leu Glu Ala Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln 35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Leu Ala Lys 50 55 60 Asp Leu Ile Cys Asp Leu Val Glu Ser Met Asn Ile Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Asp Tyr Asp Ala Ala Met Glu Cys Ile 85 90 95 Glu Val Gly Tyr Thr Ser Val Met Phe Asp Gly Ser His Leu Pro Phe 100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Glu Val Val Glu Lys Ala His Ala 115 120 125 Lys Gly Val Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu 130 135 140 Asp Gly Ile Ile Gly Ala Gly Glu Leu Ala Asp Ile Glu Glu Cys Lys 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn 165 170 175 Ile His Gly Gln Tyr Pro Glu Asn Trp Gln Gly Leu Ala Phe Asp His 180 185 190 Leu Gln Ala Ile Ala Asp Ala Val Gly Ser Asp Val Pro Leu Val Leu 195 200 205 His Gly Gly Ser Gly Ile Pro Gln Glu Gln Ile Glu Lys Ala Ile Ser 210 215 220 Met Gly Val Ser Lys Val Asn Val Asn Thr Glu Phe Gln Leu Ser Phe 225 230 235 240 Ala Lys Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Gln Glu Gly 245 250 255 Lys Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Lys Ala Ala Ile 260 265 270 Ile Lys Asp Ala Glu Glu His Ile Asp Trp Phe Gly Ser Ala Gly Lys 275 280 285 Ala <210> 37 <211> 289 <212> PRT <213> Enterococcus sp. <400> 37 Met Pro Val Val Ser Gly Ala Glu Phe Leu Lys Ala Ala Arg Gln Gly 1 5 10 15 Gly Tyr Ala Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Gln 20 25 30 Ala Ile Leu Lys Ala Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln 35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Val Ala Lys 50 55 60 Asp Ile Val Thr Asn Leu Val Asp Ser Met Asn Ile Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Asp Tyr Asp Ala Ala Met Glu Cys Ile 85 90 95 Glu Val Gly Tyr Thr Ser Val Met Phe Asp Gly Ser His Leu Pro Phe 100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Glu Val Val Glu Lys Ala His Ala 115 120 125 Lys Gly Val Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu 130 135 140 Asp Gly Ile Ile Gly Ser Gly Glu Leu Ala Asp Ile Glu Glu Cys Lys 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn 165 170 175 Ile His Gly Gln Tyr Pro Glu Asn Trp Lys Gly Leu Ala Phe Asp His 180 185 190 Leu Gln Ala Ile Ala Asp Ala Val Gly Ser Asp Val Pro Leu Val Leu 195 200 205 His Gly Gly Ser Gly Ile Pro Gln Asp Gln Ile Glu Lys Ala Ile Ser 210 215 220 Met Gly Val Ser Lys Val Asn Val Asn Thr Glu Phe Gln Leu Ser Phe 225 230 235 240 Ala Lys Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Lys Glu Gly 245 250 255 Lys Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Val Ala Ala Ile 260 265 270 Val Lys Asp Ala Glu Glu His Ile Asp Trp Phe Gly Ser Ala Gly Lys 275 280 285 Ala <210> 38 <211> 289 <212> PRT <213> Enterococcus villorum <400> 38 Met Pro Val Val Ser Gly Ala Glu Phe Leu Lys Ala Ala Arg Lys Gly 1 5 10 15 Gly Tyr Ala Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Gln 20 25 30 Ala Ile Leu Glu Ala Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln 35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Val Ala Lys 50 55 60 Asp Ile Ile Cys Asp Leu Val Glu Ser Met Asn Ile Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Asp Tyr Asp Ala Ala Met Glu Cys Ile 85 90 95 Glu Val Gly Tyr Thr Ser Val Met Phe Asp Gly Ser His Leu Pro Phe 100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Glu Val Val Glu Lys Ala His Ala 115 120 125 Lys Gly Ile Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu 130 135 140 Asp Gly Ile Ile Gly Ser Gly Glu Leu Ala Asp Ile Glu Glu Cys Lys 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn 165 170 175 Ile His Gly Gln Tyr Pro Glu Asn Trp Ala Gly Leu Ala Phe Asp His 180 185 190 Leu Gln Ala Ile Ala Asp Ala Val Gly Ser Asp Val Pro Leu Val Leu 195 200 205 His Gly Gly Ser Gly Ile Pro Gln Glu Gln Ile Gln Lys Ala Ile Ser 210 215 220 Met Gly Val Ser Lys Val Asn Val Asn Thr Glu Phe Gln Leu Ser Phe 225 230 235 240 Ala Lys Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Lys Glu Gly 245 250 255 Lys Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Lys Ala Ala Ile 260 265 270 Ile Lys Asp Ala Glu Glu His Ile Asp Trp Phe Gly Ser Ala Asn Lys 275 280 285 Ala <210> 39 <211> 350 <212> PRT <213> Escherichia coli <400> 39 Met Thr Asp Ile Ala Gln Leu Leu Gly Lys Asp Ala Asp Asn Leu Leu 1 5 10 15 Gln His Arg Cys Met Thr Ile Pro Ser Asp Gln Leu Tyr Leu Pro Gly 20 25 30 His Asp Tyr Val Asp Arg Val Met Ile Asp Asn Asn Arg Pro Pro Ala 35 40 45 Val Leu Arg Asn Met Gln Thr Leu Tyr Asn Thr Gly Arg Leu Ala Gly 50 55 60 Thr Gly Tyr Leu Ser Ile Leu Pro Val Asp Gln Gly Val Glu His Ser 65 70 75 80 Ala Gly Ala Ser Phe Ala Ala Asn Pro Leu Tyr Phe Asp Pro Lys Asn 85 90 95 Ile Val Glu Leu Ala Ile Glu Ala Gly Cys Asn Cys Val Ala Ser Thr 100 105 110 Tyr Gly Val Leu Ala Ser Val Ser Arg Arg Tyr Ala His Arg Ile Pro 115 120 125 Phe Leu Val Lys Leu Asn His Asn Glu Thr Leu Ser Tyr Pro Asn Thr 130 135 140 Tyr Asp Gln Thr Leu Tyr Ala Ser Val Glu Gln Ala Phe Asn Met Gly 145 150 155 160 Ala Val Ala Val Gly Ala Thr Ile Tyr Phe Gly Ser Glu Glu Ser Arg 165 170 175 Arg Gln Ile Glu Glu Ile Ser Ala Ala Phe Glu Arg Ala His Glu Leu 180 185 190 Gly Met Val Thr Val Leu Trp Ala Tyr Leu Arg Asn Ser Ala Phe Lys 195 200 205 Lys Asp Gly Val Asp Tyr His Val Ser Ala Asp Leu Thr Gly Gln Ala 210 215 220 Asn His Leu Ala Ala Thr Ile Gly Ala Asp Ile Val Lys Gln Lys Met 225 230 235 240 Ala Glu Asn Asn Gly Gly Tyr Lys Ala Ile Asn Tyr Gly Tyr Thr Asp 245 250 255 Asp Arg Val Tyr Ser Lys Leu Thr Ser Glu Asn Pro Ile Asp Leu Val 260 265 270 Arg Tyr Gln Leu Ala Asn Cys Tyr Met Gly Arg Ala Gly Leu Ile Asn 275 280 285 Ser Gly Gly Ala Ala Gly Gly Glu Thr Asp Leu Ser Asp Ala Val Arg 290 295 300 Thr Ala Val Ile Asn Lys Arg Ala Gly Gly Met Gly Leu Ile Leu Gly 305 310 315 320 Arg Lys Ala Phe Lys Lys Ser Met Ala Asp Gly Val Lys Leu Ile Asn 325 330 335 Ala Val Gln Asp Val Tyr Leu Asp Ser Lys Ile Thr Ile Ala 340 345 350 <210> 40 <211> 359 <212> PRT <213> Escherichia coli <400> 40 Met Ser Lys Ile Phe Asp Phe Val Lys Pro Gly Val Ile Thr Gly Asp 1 5 10 15 Asp Val Gln Lys Val Phe Gln Val Ala Lys Glu Asn Asn Phe Ala Leu 20 25 30 Pro Ala Val Asn Cys Val Gly Thr Asp Ser Ile Asn Ala Val Leu Glu 35 40 45 Thr Ala Ala Lys Val Lys Ala Pro Val Ile Val Gln Phe Ser Asn Gly 50 55 60 Gly Ala Ser Phe Ile Ala Gly Lys Gly Val Lys Ser Asp Val Pro Gln 65 70 75 80 Gly Ala Ala Ile Leu Gly Ala Ile Ser Gly Ala His His Val His Gln 85 90 95 Met Ala Glu His Tyr Gly Val Pro Val Ile Leu His Thr Asp His Cys 100 105 110 Ala Lys Lys Leu Leu Pro Trp Ile Asp Gly Leu Leu Asp Ala Gly Glu 115 120 125 Lys His Phe Ala Ala Thr Gly Lys Pro Leu Phe Ser Ser His Met Ile 130 135 140 Asp Leu Ser Glu Glu Ser Leu Gln Glu Asn Ile Glu Ile Cys Ser Lys 145 150 155 160 Tyr Leu Glu Arg Met Ser Lys Ile Gly Met Thr Leu Glu Ile Glu Leu 165 170 175 Gly Cys Thr Gly Gly Glu Glu Asp Gly Val Asp Asn Ser His Met Asp 180 185 190 Ala Ser Ala Leu Tyr Thr Gln Pro Glu Asp Val Asp Tyr Ala Tyr Thr 195 200 205 Glu Leu Ser Lys Ile Ser Pro Arg Phe Thr Ile Ala Ala Ser Phe Gly 210 215 220 Asn Val His Gly Val Tyr Lys Pro Gly Asn Val Val Leu Thr Pro Thr 225 230 235 240 Ile Leu Arg Asp Ser Gln Glu Tyr Val Ser Lys Lys His Asn Leu Pro 245 250 255 His Asn Ser Leu Asn Phe Val Phe His Gly Gly Ser Gly Ser Thr Ala 260 265 270 Gln Glu Ile Lys Asp Ser Val Ser Tyr Gly Val Val Lys Met Asn Ile 275 280 285 Asp Thr Asp Thr Gln Trp Ala Thr Trp Glu Gly Val Leu Asn Tyr Tyr 290 295 300 Lys Ala Asn Glu Ala Tyr Leu Gln Gly Gln Leu Gly Asn Pro Lys Gly 305 310 315 320 Glu Asp Gln Pro Asn Lys Lys Tyr Tyr Asp Pro Arg Val Trp Leu Arg 325 330 335 Ala Gly Gln Thr Ser Met Ile Ala Arg Leu Glu Lys Ala Phe Gln Glu 340 345 350 Leu Asn Ala Ile Asp Val Leu 355 <210> 41 <211> 387 <212> PRT <213> Escherichia sp. <400> 41 Met Ile Gly Val Ser Glu Arg Arg Arg Ser Thr Cys Asn Leu Lys Tyr 1 5 10 15 Asp Glu Tyr Lys Ala Arg Arg Tyr Arg Thr Arg Asp Met Ser Lys Ile 20 25 30 Phe Asp Phe Val Lys Pro Gly Val Ile Thr Gly Asp Asp Val Gln Lys 35 40 45 Val Phe Gln Val Ala Lys Glu Asn Asn Phe Ala Leu Pro Ala Val Asn 50 55 60 Cys Val Gly Thr Asp Ser Ile Asn Ala Val Leu Glu Thr Ala Ala Lys 65 70 75 80 Val Lys Ala Pro Val Ile Val Gln Phe Ser Asn Gly Gly Ala Ser Phe 85 90 95 Ile Ala Gly Lys Gly Val Lys Ser Asp Val Pro Gln Gly Ala Ala Ile 100 105 110 Leu Gly Ala Ile Ser Gly Ala His His Val His Gln Met Ala Glu His 115 120 125 Tyr Gly Val Pro Val Ile Leu His Thr Asp His Cys Ala Lys Lys Leu 130 135 140 Leu Pro Trp Ile Asp Gly Leu Leu Asp Ala Gly Glu Lys His Phe Ala 145 150 155 160 Ala Thr Gly Lys Pro Leu Phe Ser Ser His Met Ile Asp Leu Ser Glu 165 170 175 Glu Ser Leu Gln Glu Asn Ile Glu Ile Cys Ser Lys Tyr Leu Glu Arg 180 185 190 Met Ser Lys Ile Gly Met Thr Leu Glu Ile Glu Leu Gly Cys Thr Gly 195 200 205 Gly Glu Glu Asp Gly Val Asp Asn Ser His Met Asp Ala Ser Ala Leu 210 215 220 Tyr Thr Gln Pro Glu Asp Val Asp Tyr Ala Tyr Thr Glu Leu Ser Lys 225 230 235 240 Ile Ser Pro Arg Phe Thr Ile Ala Ala Ser Phe Gly Asn Val His Gly 245 250 255 Val Tyr Lys Pro Gly Asn Val Val Leu Thr Pro Thr Ile Leu Arg Asp 260 265 270 Ser Gln Glu Tyr Val Ser Lys Lys His Asn Leu Pro His Asn Ser Leu 275 280 285 Asn Phe Val Phe His Gly Gly Ser Gly Ser Thr Ala Gln Glu Ile Lys 290 295 300 Asp Ser Val Ser Tyr Gly Val Val Lys Met Asn Ile Asp Thr Asp Thr 305 310 315 320 Gln Trp Ala Thr Trp Glu Gly Val Leu Asn Tyr Tyr Lys Glu Asn Glu 325 330 335 Ala Tyr Leu Gln Gly Gln Leu Gly Asn Pro Lys Gly Glu Asp Gln Pro 340 345 350 Asn Lys Lys Tyr Tyr Asp Pro Arg Val Trp Leu Arg Ala Gly Gln Thr 355 360 365 Ser Met Ile Ala Arg Leu Glu Lys Ala Phe Gln Glu Leu Asn Ala Ile 370 375 380 Asp Val Leu 385 <210> 42 <211> 295 <212> PRT <213> Geobacillus kaustophilus <400> 42 Met Pro Leu Val Ser Met Lys Asn Met Leu Gln Arg Ala Trp Gln Gly 1 5 10 15 Arg Tyr Ala Val Gly His Phe Asn Ile Asn Asn Leu Glu Phe Ala Gln 20 25 30 Ala Ile Leu Leu Gly Ala Glu Glu Glu Glu Ala Pro Val Ile Leu Ala 35 40 45 Val Ser Pro Gly Tyr Ile Asp His Leu Gly Gly Leu Arg Thr Ala Ala 50 55 60 Ala Met Val Lys Glu Leu Val Lys Glu Tyr Arg Ile Thr Val Pro Val 65 70 75 80 Ala Leu His Leu Asp His Gly Ser Ser Tyr Glu Gln Cys Leu Glu Ala 85 90 95 Met Glu Ala Gly Phe Thr Ser Val Met Ile Asp Ala Ser His His Pro 100 105 110 Leu Glu Gln Asn Ile Ser Ile Thr Lys Gln Val Val Lys Ala Ala Arg 115 120 125 Glu Phe Gly Val Ser Val Glu Ala Glu Val Gly Arg Ile Gly Gly Gln 130 135 140 Glu Asp Asp Leu Val Val Asp Glu Ala Glu Ala Met Tyr Ala Ile Pro 145 150 155 160 Glu Glu Cys Glu Arg Leu Val Lys Glu Thr Gly Val Asp Cys Leu Ala 165 170 175 Pro Ala Leu Gly Ser Val His Gly Pro Tyr Lys Gly Lys Pro Lys Leu 180 185 190 Gly Phe Ala Gln Met Glu Glu Ile Gly Arg Arg Thr Gly Val Pro Leu 195 200 205 Val Leu His Gly Gly Thr Gly Ile Pro Leu Ala Asp Ile Gln Lys Ala 210 215 220 Ile Ser Phe Gly Thr Ala Lys Ile Asn Val Asn Thr Glu Asn Gln Trp 225 230 235 240 Ala Phe Thr Arg Gly Ile Cys Arg Met Phe Arg Glu Asn Glu Gly Leu 245 250 255 Tyr Asp Pro Arg Lys Tyr Leu Gly Ala Gly Arg Glu Glu Val Lys Gln 260 265 270 Thr Val Lys Gln Lys Ile Arg Glu Phe Gly Ser Ala Lys Lys Ala Thr 275 280 285 Glu Ala Leu Leu Pro Ser Val 290 295 <210> 43 <211> 287 <212> PRT <213> Geobacillus sp. <400> 43 Met Pro Leu Val Ser Met Lys Glu Met Leu Asn Glu Ala Leu Arg Gly 1 5 10 15 Lys Tyr Ala Val Gly Gln Phe Asn Ile Asn Asn Leu Glu Trp Thr Gln 20 25 30 Ala Ile Leu Ala Ala Ala Glu Glu Glu Lys Ser Pro Val Ile Leu Gly 35 40 45 Val Ser Glu Gly Ala Ala Arg Tyr Met Ser Gly Phe Lys Thr Val Val 50 55 60 Asn Met Val Lys Gly Leu Met Glu Asp Met Asn Ile Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Lys Cys Lys Ala Ala 85 90 95 Ile Asp Ala Gly Phe Thr Ser Val Met Ile Asp Ala Ser His His Pro 100 105 110 Phe Glu Glu Asn Val Arg Ile Thr Ser Gln Val Val Glu Tyr Ala His 115 120 125 Ala Arg Gly Val Ser Val Glu Ala Glu Leu Gly Ile Val Gly Gly Gln 130 135 140 Glu Asp Asp Val Val Gly Glu Gly Ile Ile Tyr Ala Asp Pro Lys Glu 145 150 155 160 Cys Glu Glu Leu Val Lys Arg Thr Gly Val Asp Cys Leu Ala Pro Ala 165 170 175 Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Lys Leu Gly Phe 180 185 190 Ala Glu Met Glu Gln Ile Arg Asp Leu Thr Gly Val Pro Leu Val Leu 195 200 205 His Gly Gly Thr Gly Ile Pro Thr Glu Gln Ile Gln Arg Ala Ile Ser 210 215 220 Leu Gly Thr Ser Lys Ile Asn Val Asn Thr Glu Asn Gln Met Ala Phe 225 230 235 240 Thr Lys Val Val Arg Glu Leu Leu Ala Lys Asp Pro Asn Val Tyr Asp 245 250 255 Pro Arg Lys Ile Ile Gly Pro Gly Arg Asp Ala Ile Lys Ala Thr Val 260 265 270 Ile Gly Lys Met Arg Glu Phe Gly Ser Ser Gly Lys Ala Ala Arg 275 280 285 <210> 44 <211> 287 <212> PRT <213> Geobacillus stearothermophilus <400> 44 Met Ser Leu Val Ser Met Lys Glu Met Leu Asn Glu Ala Leu Arg Gly 1 5 10 15 Lys Tyr Ala Val Gly Gln Phe Asn Ile Asn Asn Leu Glu Trp Thr Gln 20 25 30 Ala Ile Leu Ala Ala Ala Glu Glu Glu Lys Ser Pro Val Ile Leu Gly 35 40 45 Val Ser Glu Gly Ala Ala Arg Tyr Met Gly Gly Phe Lys Thr Val Val 50 55 60 Asn Met Val Lys Gly Leu Met Glu Asp Met Asn Ile Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Lys Cys Lys Ala Ala 85 90 95 Ile Asp Ala Gly Phe Thr Ser Val Met Ile Asp Ala Ser His His Pro 100 105 110 Phe Glu Glu Asn Val Arg Ile Thr Ser Gln Val Val Glu Tyr Ala His 115 120 125 Ala Arg Gly Val Ser Val Glu Ala Glu Leu Gly Ile Val Gly Gly Gln 130 135 140 Glu Asp Asp Val Val Gly Glu Gly Val Ile Tyr Ala Asp Pro Lys Glu 145 150 155 160 Cys Glu Glu Leu Val Lys Arg Thr Gly Ile Asp Cys Leu Ala Pro Ala 165 170 175 Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Lys Leu Gly Phe 180 185 190 Ala Glu Met Glu Lys Ile Arg Asp Leu Thr Gly Ile Pro Leu Val Leu 195 200 205 His Gly Gly Thr Gly Ile Pro Thr Glu Gln Ile Gln Arg Ala Ile Ser 210 215 220 Leu Gly Thr Ser Lys Ile Asn Val Asn Thr Glu Asn Gln Ile Ala Phe 225 230 235 240 Thr Lys Ala Val Arg Glu Leu Leu Ala Lys Asp Pro Asn Val Tyr Asp 245 250 255 Pro Arg Lys Ile Ile Gly Pro Gly Arg Asp Ala Ile Lys Ala Thr Val 260 265 270 Ile Gly Lys Met Arg Glu Phe Gly Ser Ser Gly Lys Ala Ala Gln 275 280 285 <210> 45 <211> 295 <212> PRT <213> Geobacillus thermodenitrificans <400> 45 Met Pro Leu Val Ser Met Lys Glu Met Leu Gln Cys Ala Leu Gln Gly 1 5 10 15 Asn Tyr Ala Val Gly His Phe Asn Val Asn Asn Leu Glu Phe Ala Gln 20 25 30 Ala Ile Leu Leu Gly Ala Glu Glu Glu Glu Ala Pro Val Ile Leu Ala 35 40 45 Val Ser Pro Gly Tyr Ile Gly His Leu Gly Gly Leu Arg Thr Ala Ala 50 55 60 Ala Met Val Lys Glu Leu Val Lys Glu Tyr Arg Ile Thr Val Pro Val 65 70 75 80 Ala Leu His Leu Asp His Gly Ser Ser Tyr Glu Gln Cys Leu Glu Ala 85 90 95 Met Glu Ala Gly Phe Thr Ser Val Met Ile Asp Ala Ser His Phe Pro 100 105 110 Leu Glu Glu Asn Ile Ser Met Thr Lys Lys Val Val Glu Ala Ala Arg 115 120 125 Phe Phe Gly Val Ser Val Glu Ala Glu Val Gly Arg Ile Gly Gly Gln 130 135 140 Glu Asp Asp Val Val Val Asp Glu Ala Glu Ala Met Tyr Ala Ile Pro 145 150 155 160 Glu Glu Cys Glu Arg Leu Val Lys Glu Thr Gly Val Asp Cys Leu Ala 165 170 175 Pro Ala Leu Gly Ser Val His Gly Pro Tyr Lys Gly Lys Pro Lys Leu 180 185 190 Gly Phe Ala Gln Met Glu Gln Ile Gln Arg Leu Thr Gly Val Pro Leu 195 200 205 Val Leu His Gly Gly Thr Gly Ile Pro Leu Glu Asp Ile Arg Arg Ala 210 215 220 Ile Ser Leu Gly Thr Ala Lys Ile Asn Val Asn Thr Glu Asn Gln Leu 225 230 235 240 Ala Phe Thr Arg Gly Val Arg Ser Leu Leu Asn Glu Asn Asn Gly Leu 245 250 255 Tyr Asp Pro Arg Lys Tyr Leu Gly Ala Gly Arg Glu Glu Val Lys Gln 260 265 270 Thr Val Arg Gln Lys Ile Arg Glu Phe Gly Ser Val Gly Lys Ala Lys 275 280 285 Glu Gly Val Leu Ser Ser Leu 290 295 <210> 46 <211> 303 <212> PRT <213> Lactobacillus acidophilus <400> 46 Met Ala Tyr Phe Val Asn Gly Asn Glu Ile Phe Arg Glu Ala Arg Lys 1 5 10 15 His His Tyr Ala Val Gly Ala Tyr Asn Thr Asn Asn Leu Glu Trp Thr 20 25 30 Arg Ala Glu Leu Arg Ala Ala Glu Glu Thr Arg Thr Pro Leu Leu Ile 35 40 45 Gln Val Ser Thr Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Phe Val 50 55 60 Lys Asp Met Val Glu Asp Gln Met Asp Ser Met Asn Ile Ser Val Pro 65 70 75 80 Val Ile Leu Asn Leu Asp His Ser Asp Phe Glu Ser Ala Lys Glu Cys 85 90 95 Ile Ala Leu Gly Tyr Ser Ser Val Met Phe Asp Gly His Ala Leu Pro 100 105 110 Thr Glu Glu Asn Leu Ala Lys Thr Lys Glu Ile Ile Lys Leu Ala His 115 120 125 Glu Arg Gly Ile Ser Val Glu Ala Glu Ile Gly Lys Ile Gly Glu Asn 130 135 140 Gln Gly Gly Gly Glu Leu Ala Ser Val Glu Asp Ala Lys Ala Phe Val 145 150 155 160 Glu Ala Gly Val Asp Lys Leu Ala Cys Gly Ile Gly Asn Ile His Gly 165 170 175 Val Tyr Pro Ala Asp Trp Lys Gly Leu Asn Phe Asp Arg Leu Lys Glu 180 185 190 Ile Ala Glu Ala Val Pro Glu Thr Pro Leu Val Leu His Gly Gly Ser 195 200 205 Gly Ile Pro Glu Asp Gln Ile Lys Lys Ala Ile Gln Leu Gly Ile Ser 210 215 220 Lys Val Asn Ile Asn Thr Glu Phe Gln Leu Ala Phe Gln Glu Ala Thr 225 230 235 240 Arg Lys Tyr Ile Glu Glu Gly Lys Asp Glu Asp Lys Ala Asn Lys Gly 245 250 255 Tyr Asp Pro Arg Lys Leu Leu Leu Pro Gly Thr Glu Ala Ile Thr Asp 260 265 270 Lys Met Lys Glu Met Ile Ala Trp Leu Gly Thr Lys Thr Ile Asp Asp 275 280 285 Glu Leu Lys Asp Ala Ser Phe Asp Arg Ser Ser Leu Asn Glu Glu 290 295 300 <210> 47 <211> 304 <212> PRT <213> Lactobacillus amylolyticus <400> 47 Met Ala Tyr Phe Asp Asn Gly Asn Gln Ile Phe Lys Asp Ala Arg Arg 1 5 10 15 Glu His Tyr Ala Val Gly Ala Tyr Asn Thr Asn Asn Leu Glu Trp Thr 20 25 30 Arg Ala Glu Leu Arg Ala Ala Glu Glu Thr Arg Thr Pro Leu Leu Ile 35 40 45 Gln Val Ser Thr Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Phe Val 50 55 60 Lys Asp Met Val Ala Asp Gln Ile Asp Ser Met Asn Ile Ser Val Pro 65 70 75 80 Val Ile Leu Asn Leu Asp His Gly Asp Tyr Glu Ser Ala Lys Glu Cys 85 90 95 Ile Ala Leu Gly Tyr Ser Ser Val Met Phe Asp Gly His Ala Leu Pro 100 105 110 Val Glu Glu Asn Leu Ala Lys Thr Lys Glu Ile Ile Lys Leu Ala His 115 120 125 Glu Arg Gly Ile Ser Val Glu Ala Glu Ile Gly Lys Ile Gly Glu Asn 130 135 140 Gln Gly Ala Asp Gly Gly Glu Leu Ala Ser Val Glu Asp Ala Ile Thr 145 150 155 160 Phe Tyr Lys Ala Gly Val Asp Lys Leu Ala Cys Gly Ile Gly Asn Ile 165 170 175 His Gly Val Tyr Pro Glu Gly Trp Lys Gly Leu Asn Phe Asp Arg Leu 180 185 190 Lys Glu Ile Ala Asp Ala Val Pro Val Pro Leu Val Leu His Gly Gly 195 200 205 Ser Gly Ile Pro Glu Asp Gln Val Lys Lys Ala Ile Ser Leu Gly Ile 210 215 220 Ala Lys Val Asn Ile Asn Thr Glu Phe Gln Leu Ala Phe Gln Lys Ala 225 230 235 240 Thr Arg Lys Tyr Ile Glu Asp Lys Lys Asp Leu Asp Thr Ala Asn Lys 245 250 255 Gly Tyr Asp Pro Arg Lys Leu Leu Leu Pro Gly Thr Glu Ala Ile Thr 260 265 270 Ala Ala Met Lys Glu Met Ile Ser Trp Leu Gly Thr Lys Ser Ile Asp 275 280 285 Asp Glu Leu Lys Asp Ala Ser Phe Asp Arg Ala Ser Leu Asn Glu Glu 290 295 300 <210> 48 <211> 295 <212> PRT <213> Lactobacillus antri <400> 48 Met Arg Lys Glu Val Asn Thr Met Ala Tyr Phe Asp Asn Gly Asn Gln 1 5 10 15 Ile Phe Ala Asp Ala Arg Ala Asn His Tyr Ala Val Gly Ala Tyr Asn 20 25 30 Ile Asn Asn Leu Glu Trp Thr Arg Ala Ile Leu Gln Ala Ala Asn Glu 35 40 45 Thr Arg Thr Pro Val Leu Val Gln Val Ser Met Gly Ala Ala Lys Tyr 50 55 60 Met Gly Gly Phe Lys Phe Val Arg Asp Met Val Ala Asp Gln Ile Asp 65 70 75 80 Ala Met Gly Ile Thr Val Pro Val Val Leu Asn Leu Asp His Gly Asp 85 90 95 Phe Ala Ala Ala Met Glu Cys Ile Asp Leu Gly Tyr Ser Ser Val Met 100 105 110 Tyr Asp Gly His Lys Leu Pro Leu Ala Thr Asn Tyr Asp Gln Thr Ala 115 120 125 Gln Ile Val Gln Ala Ala His Gln Arg Gly Ile Ser Val Glu Ala Glu 130 135 140 Ile Gly Lys Val Gly Glu Asn Gln Asp Thr Asn Ser Gly Glu Leu Ala 145 150 155 160 Ser Val Ala Asp Ala Lys Lys Phe Ala Glu Leu Gly Val Asp Lys Leu 165 170 175 Ala Cys Gly Ile Gly Asn Ile His Gly Ile Tyr Pro Ala Asp Trp Arg 180 185 190 Gly Leu Asp Phe Asp Arg Leu Ala Ala Ile Ala Asp Ala Val Asp Ile 195 200 205 Pro Leu Val Leu His Gly Gly Ser Gly Ile Pro Lys Asp Gln Val Gln 210 215 220 Arg Ala Ile Ala Leu Gly Ile Ala Lys Val Asn Ile Asn Thr Glu Phe 225 230 235 240 Gln Leu Ala Phe Gln Ala Ala Thr Arg Asp Tyr Ile Glu Gln Ala Arg 245 250 255 Asp Leu Asp Gln Ala Gln Lys Gly Tyr Asp Pro Arg Lys Leu Leu Leu 260 265 270 Pro Gly Thr Glu Ala Ile Thr Ala Lys Met Lys Glu Ile Ile Asp Trp 275 280 285 Leu Gly Thr Pro Ala Val Ala 290 295 <210> 49 <211> 293 <212> PRT <213> Lactobacillus casei <400> 49 Met Pro Leu Val Asn Ala Ala Glu Leu Val Lys Ala Ala His Lys Gly 1 5 10 15 His Tyr Cys Ile Gly Ala Phe Asn Thr Asn Asn Leu Glu Trp Thr Arg 20 25 30 Ala Ile Leu Ala Gly Ala Gln Glu Leu Asn Val Pro Val Ile Ile Gln 35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Glu Phe Cys Gln 50 55 60 Thr Met Ile Glu Ala Thr Val Lys Ala Met Gly Ile Asn Val Pro Val 65 70 75 80 Val Ile His Leu Asp His Gly Asn Tyr Glu Ala Ala Lys Glu Ala Ile 85 90 95 Ala Ala Gly Tyr Asn Ser Val Met Phe Asp Gly His Asp Leu Asp Phe 100 105 110 Glu Asp Asn Leu Glu Lys Thr Lys Glu Ile Val Lys Leu Ala His Ala 115 120 125 Lys Gly Ile Ser Val Glu Ala Glu Val Gly Ser Ile Gly Gly Glu Glu 130 135 140 Asp Gly Val Val Gly Glu Gly Glu Leu Ala Asp Val Glu Glu Ala Lys 145 150 155 160 Thr Leu Ala Ala Thr Gly Ile Asp Phe Leu Ala Ala Gly Ile Gly Asn 165 170 175 Ile His Gly Gln Tyr Pro Asp Asn Trp Lys Gly Leu His Phe Asp Arg 180 185 190 Leu Gln Glu Leu Asn Asp Ala Val Lys Met Pro Leu Val Leu His Gly 195 200 205 Gly Ser Gly Ile Pro Gln Glu Gln Val Gln Lys Ala Ile Thr Met Gly 210 215 220 Ile Ser Lys Leu Asn Ile Asn Thr Glu Cys Gln Leu Ala Phe Ala Lys 225 230 235 240 Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Gln Gln Gly Lys Gly 245 250 255 Phe Asp Pro Arg Lys Leu Leu Lys Pro Gly Thr Asp Ala Ile Thr Asp 260 265 270 Thr Phe Lys Glu Ile Thr Gly Trp Ile Gly Asn Lys Pro Val Lys Met 275 280 285 Val Pro Glu Ala Leu 290 <210> 50 <211> 303 <212> PRT <213> Lactobacillus crispatus <400> 50 Met Ala Tyr Phe Asp Asn Gly Asn Gln Ile Phe Lys Asp Ala Arg Lys 1 5 10 15 Asn His Tyr Ala Val Gly Ala Tyr Asn Thr Asn Asn Leu Glu Trp Thr 20 25 30 Arg Ala Glu Leu Arg Ala Ala Glu Glu Thr Arg Thr Pro Leu Leu Ile 35 40 45 Gln Val Ser Thr Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Phe Val 50 55 60 Lys Asp Met Val Ala Asp Gln Met Asp Ser Met Asn Ile Ser Val Pro 65 70 75 80 Val Ile Leu Asn Leu Asp His Gly Asp Phe Glu Ser Ala Lys Glu Cys 85 90 95 Ile Ala Leu Gly Tyr Ser Ser Val Met Phe Asp Gly His Ala Leu Pro 100 105 110 Thr Asp Glu Asn Leu Glu Lys Thr Lys Glu Ile Ile Lys Leu Ala His 115 120 125 Glu Arg Gly Ile Ser Val Glu Ala Glu Ile Gly Lys Ile Gly Glu Asn 130 135 140 Gln Gly Gly Gly Glu Leu Ala Ser Val Glu Asp Ala Lys Lys Phe Val 145 150 155 160 Ala Ala Gly Val Asp Lys Leu Ala Cys Gly Ile Gly Asn Ile His Gly 165 170 175 Val Tyr Pro Ala Asp Trp Lys Gly Leu Asn Phe Asp Arg Leu Lys Glu 180 185 190 Ile Ala Glu Ala Val Pro Asp Thr Pro Leu Val Leu His Gly Gly Ser 195 200 205 Gly Ile Pro Glu Asp Gln Val Lys Lys Ala Ile Lys Leu Gly Ile Ala 210 215 220 Lys Ile Asn Ile Asn Thr Glu Phe Gln Leu Ala Phe Gln Glu Ala Thr 225 230 235 240 Arg Lys Tyr Ile Glu Asp Glu Lys Asp Leu Asp Lys Lys Ala Lys Gly 245 250 255 Tyr Asp Pro Arg Lys Leu Leu Leu Pro Gly Ala Glu Ala Ile Thr Ala 260 265 270 Lys Met Lys Glu Met Ile Ser Trp Met Gly Thr Lys Thr Ile Asp Asp 275 280 285 Glu Leu Lys Asp Ala Ser Phe Asp Arg Ser Ser Leu Asn Glu Glu 290 295 300 <210> 51 <211> 287 <212> PRT <213> Lactobacillus curvatus <400> 51 Met Ser Leu Val Asn Gly Thr Glu Ile Phe Lys Ala Ala Arg Glu Gly 1 5 10 15 His Tyr Ala Val Gly Ala Phe Asn Thr Asn Asn Leu Glu Trp Thr Arg 20 25 30 Ala Ile Leu Gly Ala Ala Gln Glu Thr Asn Thr Pro Ile Leu Ile Gln 35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Glu Leu Cys Lys 50 55 60 Asn Leu Val Glu Gln Thr Ile Lys Ser Met Asn Ile Thr Val Pro Val 65 70 75 80 Val Met His Leu Asp His Gly Asn Tyr Glu Ala Ala Lys Glu Cys Ile 85 90 95 Glu Val Gly Tyr Asn Ser Val Met Phe Asp Gly His Asp Leu Pro Phe 100 105 110 Glu Glu Asn Leu Ala Lys Thr Lys Glu Ile Val Ala Leu Ala His Ala 115 120 125 Lys Gly Ile Ser Val Glu Ala Glu Val Gly Ser Ile Gly Gly Glu Glu 130 135 140 Asp Gly Ile Ile Gly Ala Gly Glu Leu Ala Asp Val Glu Glu Ala Lys 145 150 155 160 Gln Leu Ala Ala Ala Gly Pro Asp Tyr Leu Ala Val Gly Ile Gly Asn 165 170 175 Ile His Gly Val Tyr Pro Glu Asn Trp Gln Gly Leu Ser Phe Asp Arg 180 185 190 Leu Gln Glu Leu Ala Asp Val Ile Asp Leu Pro Leu Val Leu His Gly 195 200 205 Gly Ser Gly Ile Pro Lys Glu Gln Ile Leu Lys Ala Ile Glu Met Gly 210 215 220 Ile Ser Lys Val Asn Val Asn Thr Glu Gln Gln Ile Ala Trp Ser Asn 225 230 235 240 Ala Val Lys Glu Tyr Tyr Ala Glu Asn Lys Asp Gln Ala Glu Lys Gly 245 250 255 Phe Asp Pro Arg Lys Val Leu Ala Pro Gly Thr Lys Ala Ile Lys Asp 260 265 270 Thr Val Glu Ser Arg Ile Glu Trp Phe Gly Thr Pro Ala Val Lys 275 280 285 <210> 52 <211> 303 <212> PRT <213> Lactobacillus delbrueckii subsp. bulgaricus <400> 52 Met Ala Tyr Phe Val Asn Gly Asn Asp Ile Phe Lys Lys Ala Arg Lys 1 5 10 15 Glu His Tyr Ala Val Gly Ala Tyr Asn Thr Asn Asn Leu Glu Trp Thr 20 25 30 Arg Ala Leu Leu Arg Gly Ala Glu Glu Thr Arg Thr Pro Leu Leu Ile 35 40 45 Gln Val Ser Thr Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Leu Val 50 55 60 Lys Asp Leu Val Glu Asp Ser Met Asp Ala Met Asn Ile Ser Val Pro 65 70 75 80 Val Ile Leu Asn Leu Asp His Gly Asp Tyr Asp Ser Ala Ile Glu Cys 85 90 95 Ile Lys Leu Gly Tyr Ser Ser Val Met Phe Asp Gly His Ala Leu Pro 100 105 110 Thr Glu Glu Asn Leu Ala Lys Thr Lys Glu Ile Ile Lys Leu Ala His 115 120 125 Glu Arg Gly Ile Ser Val Glu Ala Glu Ile Gly Lys Ile Gly Glu Asn 130 135 140 Gln Gly Ala Gly Glu Leu Ala Ser Val Glu Asp Ala Lys Ala Phe Val 145 150 155 160 Ala Ala Gly Val Asp Lys Leu Ala Cys Gly Ile Gly Asn Ile His Gly 165 170 175 Val Tyr Pro Glu Gly Trp Ser Gly Leu Asn Phe Asp Arg Leu Lys Glu 180 185 190 Ile Ala Glu Ala Val Pro Glu Thr Pro Leu Val Leu His Gly Gly Ser 195 200 205 Gly Ile Pro Gln Asp Gln Ile Glu Lys Ala Ile Ser Leu Gly Ile Ser 210 215 220 Lys Ile Asn Ile Asn Thr Glu Phe Gln Leu Ala Phe Gln Glu Ala Thr 225 230 235 240 Arg Lys Tyr Ile Glu Glu Gly Lys Asp Gln Asp Lys Ser Lys Lys Gly 245 250 255 Tyr Asp Pro Arg Lys Leu Leu Leu Pro Gly Thr Glu Ala Ile Thr Asp 260 265 270 Ser Met Lys Glu Met Ile Ser Trp Met Gly Thr Pro Ser Ile Asp Asp 275 280 285 Lys Glu Ala Asp Ala Ser Phe Asp Arg Ser Ser Leu Asn Glu Glu 290 295 300 <210> 53 <211> 303 <212> PRT <213> Lactobacillus helveticus <400> 53 Met Ala Tyr Phe Asp Asn Gly Asn Glu Ile Phe Lys Glu Ala Arg Lys 1 5 10 15 His His Tyr Ala Val Gly Ala Tyr Asn Thr Asn Asn Leu Glu Trp Thr 20 25 30 Arg Ala Glu Leu Arg Ala Ala Glu Glu Thr Arg Thr Pro Leu Leu Ile 35 40 45 Gln Val Ser Thr Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Phe Val 50 55 60 Lys Asp Met Val Ala Asp Gln Met Asp Ser Met Asn Ile Ser Val Pro 65 70 75 80 Val Ile Leu Asn Leu Asp His Gly Asp Phe Glu Ser Ala Lys Glu Cys 85 90 95 Ile Ala Leu Gly Tyr Ser Ser Val Met Phe Asp Gly His Ala Leu Pro 100 105 110 Thr Glu Glu Asn Leu Ala Lys Thr Lys Glu Ile Ile Lys Leu Ala His 115 120 125 Glu Arg Gly Ile Ser Val Glu Ala Glu Ile Gly Lys Ile Gly Glu Asn 130 135 140 Gln Gly Gly Gly Glu Leu Ala Ser Val Glu Asp Ala Lys Lys Phe Val 145 150 155 160 Ala Ala Gly Val Asp Lys Leu Ala Cys Gly Ile Gly Asn Ile His Gly 165 170 175 Val Tyr Pro Ala Asp Trp Lys Gly Leu Asn Phe Asp Arg Leu Lys Glu 180 185 190 Ile Ala Glu Ala Val Pro Asp Thr Pro Leu Val Leu His Gly Gly Ser 195 200 205 Gly Ile Pro Glu Asp Gln Val Lys Lys Ala Ile Lys Leu Gly Ile Ala 210 215 220 Lys Ile Asn Ile Asn Thr Glu Phe Gln Leu Ala Phe Gln Asp Ala Thr 225 230 235 240 Arg Lys Tyr Ile Glu Ala Glu Lys Asp Leu Asp Lys Lys Asn Lys Gly 245 250 255 Tyr Asp Pro Arg Lys Leu Leu Leu Pro Gly Ala Glu Ala Ile Thr Ala 260 265 270 Lys Met Lys Glu Met Ile Ser Trp Met Gly Thr Lys Thr Ile Asp Asp 275 280 285 Glu Leu Lys Asp Val Ser Phe Asp Arg Ser Ser Leu Asn Glu Glu 290 295 300 <210> 54 <211> 304 <212> PRT <213> Lactobacillus iners <400> 54 Met Ala Tyr Leu Val Asn Gly Asn Asp Ile Phe Lys Ala Ala Arg Lys 1 5 10 15 Asn His Tyr Ala Val Gly Ala Tyr Asn Thr Asn Asn Leu Glu Trp Thr 20 25 30 Arg Ala Leu Ile Lys Gly Ala Lys Glu Thr Arg Thr Pro Leu Leu Ile 35 40 45 Gln Val Ser Thr Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Thr Val 50 55 60 Arg Asp Leu Val Leu Asn Glu Met Asp Asn Met Asp Val Asp Val Pro 65 70 75 80 Val Ile Leu Asn Leu Asp His Gly Asp Tyr Glu Ser Ala Lys Glu Cys 85 90 95 Ile Ala Leu Gly Tyr Ser Ser Val Met Phe Asp Gly His Lys Leu Pro 100 105 110 Val Glu Glu Asn Leu Glu Lys Thr Lys Glu Ile Val Lys Leu Ala His 115 120 125 Glu Arg Gly Ile Ser Val Glu Ala Glu Ile Gly Lys Ile Gly Glu Asn 130 135 140 Gln Gly Ala Asp Gly Gly Glu Leu Ala Ser Val Glu Asp Ala Lys Arg 145 150 155 160 Phe Val Ala Ala Gly Val Asp Lys Leu Ala Cys Gly Ile Gly Asn Ile 165 170 175 His Gly Val Tyr Pro Ala Asp Trp Lys Gly Leu Asn Phe Asp Arg Leu 180 185 190 Lys Glu Ile Ala Asp Ala Val Asp Val Pro Leu Val Leu His Gly Gly 195 200 205 Ser Gly Ile Pro Gln Asp Gln Ile Lys Lys Ala Ile Ser Leu Gly Ile 210 215 220 Ala Lys Ile Asn Ile Asn Thr Glu Phe Gln Leu Ala Phe Gln Glu Ala 225 230 235 240 Thr Arg Lys Tyr Ile Glu Ala Glu Lys Asp Leu Asp Lys Ala Asn Lys 245 250 255 Gly Tyr Asp Pro Arg Lys Leu Leu Arg Ala Gly Thr Asp Ala Ile Thr 260 265 270 Glu Ser Met Lys Glu Met Ile Ser Trp Met Gly Thr Arg Pro Ile Asp 275 280 285 Gly Lys Asp Ser Val Val Lys Phe Asp Glu Ala Ser Leu Asn Glu Glu 290 295 300 <210> 55 <211> 305 <212> PRT <213> Lactobacillus johnsonii <400> 55 Met Ala Tyr Leu Val Asn Gly Asn Asp Ile Phe Lys Ala Ala Arg Glu 1 5 10 15 Asn His Tyr Ala Val Gly Ala Tyr Asn Thr Asn Asn Leu Glu Trp Thr 20 25 30 Arg Ala Leu Leu Arg Gly Ala Lys Glu Thr Arg Thr Pro Leu Leu Ile 35 40 45 Gln Val Ser Thr Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Thr Val 50 55 60 Lys Asp Leu Val Leu Asn Glu Met Asp Asn Met Asp Ile Asp Val Pro 65 70 75 80 Val Ile Leu Asn Leu Asp His Gly Asp Phe Glu Ser Ala Lys Glu Cys 85 90 95 Ile Ala Leu Gly Tyr Ser Ser Val Met Phe Asp Gly His Asn Leu Pro 100 105 110 Thr Asp Glu Asn Leu Ala Lys Thr Lys Glu Ile Val Lys Leu Ala His 115 120 125 Glu Arg Gly Ile Ser Val Glu Ala Glu Ile Gly Lys Ile Gly Glu Asn 130 135 140 Gln Gly Ala Asp Gly Gly Glu Leu Ala Ser Val Glu Asp Ala Lys Thr 145 150 155 160 Phe Val Ala Ala Gly Val Asp Lys Leu Ala Cys Gly Ile Gly Asn Ile 165 170 175 His Gly Val Tyr Pro Glu Gly Trp Thr Gly Leu Asn Phe Asp Arg Leu 180 185 190 Lys Glu Ile Ala Glu Ala Val Pro Asn Glu Pro Leu Val Leu His Gly 195 200 205 Gly Ser Gly Ile Pro Gln Asp Gln Ile Glu Lys Ala Ile Gln Leu Gly 210 215 220 Ile Ala Lys Ile Asn Ile Asn Thr Glu Phe Gln Leu Ala Phe Gln Ala 225 230 235 240 Ala Thr Arg Lys Tyr Ile Glu Asp Lys Met Asp Leu Asp Lys Gly Asn 245 250 255 Lys Gly Tyr Asp Pro Arg Lys Leu Leu Arg Ala Gly Thr Asp Ala Ile 260 265 270 Thr Asp Ser Met Lys Glu Met Ile Ser Trp Met Gly Thr Ala Pro Ile 275 280 285 Asp Thr Lys Glu Ser Ser Val Gln Phe Asp Glu Ala Ser Leu Asn Glu 290 295 300 Glu 305 <210> 56 <211> 288 <212> PRT <213> Lactobacillus murinus <400> 56 Met Ser Phe Val Asn Gly Ser Glu Ile Phe Ser Ala Ala Arg Lys Gly 1 5 10 15 His Tyr Ala Val Gly Ala Phe Asn Thr Asn Asn Leu Glu Trp Thr Arg 20 25 30 Ala Ile Leu Lys Gly Ala Gln Glu Lys Asn Val Pro Val Leu Ile Gln 35 40 45 Val Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Leu Val Arg 50 55 60 Asp Leu Val Ala Ser Glu Met Glu Ala Met Asn Ile Thr Val Pro Val 65 70 75 80 Val Met His Leu Asp His Gly Asn Tyr Glu Ala Ala Lys Glu Cys Ile 85 90 95 Glu Ala Gly Phe Ser Ser Val Met Phe Asp Gly His Asp Leu Pro Phe 100 105 110 Glu Glu Asn Leu Ala Lys Thr Lys Glu Ile Val Glu Leu Ala His Ala 115 120 125 Lys Gly Ile Ser Val Glu Ala Glu Val Gly Ser Ile Gly Gly Glu Glu 130 135 140 Asp Gly Val Val Gly Ala Gly Glu Leu Ala Asp Val Glu Glu Ala Lys 145 150 155 160 Gln Ile Ala Ala Leu Gly Val Asp Tyr Leu Ala Cys Gly Ile Gly Asn 165 170 175 Ile His Gly Gln Tyr Pro Ala Asn Trp Gln Gly Leu Asn Phe Asp Arg 180 185 190 Leu Lys Glu Leu Ala Asp Ala Met Pro Asp Met Pro Leu Val Leu His 195 200 205 Gly Gly Ser Gly Ile Pro Gln Glu Gln Val Val Lys Ala Ile Ser Met 210 215 220 Gly Val Ser Lys Leu Asn Ile Asn Thr Glu Cys Gln Ile Ala Phe Ala 225 230 235 240 Glu Ala Thr Arg Lys Tyr Ile Glu Glu Gly Lys Asp Lys Glu Gly Lys 245 250 255 Gly Tyr Asp Pro Arg Lys Leu Leu Ala Pro Gly Thr Gln Ala Ile Thr 260 265 270 Asp Thr Val Glu Glu Ile Ile Asp Trp Ile Gly Thr Pro Glu Ala Lys 275 280 285 <210> 57 <211> 293 <212> PRT <213> Lactobacillus paracasei subsp. paracasei <400> 57 Met Pro Leu Val Asn Ala Ala Glu Leu Val Lys Ala Ala His Lys Gly 1 5 10 15 His Tyr Cys Ile Gly Ala Phe Asn Thr Asn Asn Leu Glu Trp Thr Arg 20 25 30 Ala Ile Leu Ala Gly Ala Gln Glu Leu Asn Val Pro Val Ile Ile Gln 35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Glu Phe Cys Gln 50 55 60 Thr Met Ile Glu Ala Thr Val Lys Ala Met Gly Ile Thr Val Pro Val 65 70 75 80 Val Ile His Leu Asp His Gly Asn Tyr Glu Ala Ala Lys Glu Ala Ile 85 90 95 Ala Ala Gly Tyr Asn Ser Val Met Phe Asp Gly His Asp Leu Asp Phe 100 105 110 Glu Asp Asn Leu Glu Lys Thr Lys Glu Ile Val Lys Leu Ala His Ala 115 120 125 Lys Gly Ile Ser Val Glu Ala Glu Val Gly Ser Ile Gly Gly Glu Glu 130 135 140 Asp Gly Val Val Gly Glu Gly Glu Leu Ala Asp Val Glu Glu Ala Lys 145 150 155 160 Thr Leu Ala Ala Thr Gly Ile Asp Phe Leu Ala Ala Gly Ile Gly Asn 165 170 175 Ile His Gly Gln Tyr Pro Asp Asn Trp Lys Gly Leu His Phe Asp Arg 180 185 190 Leu Gln Glu Leu Asn Asp Ala Val Lys Met Pro Leu Val Leu His Gly 195 200 205 Gly Ser Gly Ile Pro Gln Glu Gln Val Gln Lys Ala Ile Thr Met Gly 210 215 220 Ile Ser Lys Leu Asn Ile Asn Thr Glu Cys Gln Leu Ala Phe Ala Lys 225 230 235 240 Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Gln Gln Gly Lys Gly 245 250 255 Phe Asp Pro Arg Lys Leu Leu Lys Pro Gly Thr Asp Ala Ile Thr Asp 260 265 270 Thr Phe Lys Glu Ile Thr Gly Trp Ile Gly Asn Lys Pro Val Lys Met 275 280 285 Val Pro Glu Ala Leu 290 <210> 58 <211> 287 <212> PRT <213> Lactobacillus plantarum subsp. plantarum <400> 58 Met Ser Phe Val Asn Ala Val Asp Met Thr Gln Asp Ala Tyr Lys Asn 1 5 10 15 His Tyr Ala Ile Gly Ala Phe Asn Thr Asn Asn Leu Glu Trp Thr Arg 20 25 30 Cys Ile Leu Lys Gly Ala Gln Asp Thr Asn Thr Pro Val Ile Ile Gln 35 40 45 Val Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Leu Val Met 50 55 60 Asp Leu Val Ala Asp Glu Met Glu Ser Met Asn Ile Thr Val Pro Val 65 70 75 80 Val Met His Leu Asp His Gly Asn Tyr Glu Ala Ala Lys Glu Cys Ile 85 90 95 Glu Val Gly Tyr Ser Ser Val Met Phe Asp Gly His Asp Leu Pro Phe 100 105 110 Ala Glu Asn Leu Glu Lys Thr Lys Glu Ile Val Lys Leu Ala His Ala 115 120 125 Lys Gly Ile Ser Val Glu Ala Glu Val Gly Ser Ile Gly Gly Glu Glu 130 135 140 Asp Gly Ile Ile Gly Glu Gly Glu Leu Ala Asp Val Glu Glu Ala Lys 145 150 155 160 Thr Leu Ala Ala Thr Gly Ile Asp Ile Leu Ala Ala Gly Ile Gly Asn 165 170 175 Ile His Gly Lys Tyr Pro Glu Asn Trp Thr Gly Leu His Phe Asp Arg 180 185 190 Leu Gln Glu Ile Ser Glu Ala Val Lys Met Pro Leu Val Leu His Gly 195 200 205 Gly Ser Gly Ile Pro Lys Asp Gln Ile Val Lys Ala Val Glu Leu Gly 210 215 220 Ile Ser Lys Val Asn Val Asn Thr Glu Asn Gln Val Ala Phe Ala Asn 225 230 235 240 Ala Thr Arg Ala Tyr Ile Glu Ser Gly Ala Asp Gln Ile Gly Lys Gly 245 250 255 Tyr Asp Pro Arg Lys Leu Leu Ala Pro Gly Lys Lys Ala Ile Val Asp 260 265 270 Val Val Thr Ser Arg Ile Gly Trp Phe Lys Thr Pro Ala Val Lys 275 280 285 <210> 59 <211> 289 <212> PRT <213> Lactobacillus reuteri <400> 59 Met Met Ala Tyr Phe Asp Asn Gly Asn Lys Leu Phe Asp Asp Ala Arg 1 5 10 15 Lys Asn Gln Tyr Ala Ile Gly Ala Tyr Asn Ile Asn Asn Leu Glu Trp 20 25 30 Thr Arg Ala Ile Leu Arg Ala Ala Ala Glu Thr Asn Thr Pro Val Leu 35 40 45 Ile Gln Val Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Phe 50 55 60 Val Lys Asp Met Val Glu Asp Gln Met Asp Ala Met Glu Ile Lys Val 65 70 75 80 Pro Val Ile Leu Asn Leu Asp His Gly Asp Tyr Asp Ala Ala Ile Glu 85 90 95 Cys Ile Asn Leu Gly Tyr Ser Ser Val Met Phe Asp Gly His Lys Leu 100 105 110 Pro Ile Glu Glu Asn Tyr Ala Lys Thr Lys Glu Ile Val Gln Leu Ala 115 120 125 His Ser Arg Gly Ile Ala Val Glu Ala Glu Ile Gly Lys Val Gly Glu 130 135 140 Asn Gln Asp Thr Ser Ser Gly Glu Leu Ala Asp Val Glu Asp Ala Met 145 150 155 160 Ala Phe Ala Ala Met Gly Val Asp Arg Leu Ala Cys Gly Ile Gly Asn 165 170 175 Ile His Gly Ile Tyr Pro Ala Asn Trp Pro Gly Leu Asp Phe Asp Arg 180 185 190 Leu Gln Glu Ile Ala Asn Ala Ile Asp Ile Pro Leu Val Leu His Gly 195 200 205 Gly Ser Gly Ile Pro Arg Glu Gln Val Gln Lys Ala Ile Ser Met Gly 210 215 220 Ile Ala Lys Val Asn Ile Asn Thr Glu Phe Gln Leu Ala Phe Gln Asp 225 230 235 240 Ala Thr Arg Lys Tyr Ile Glu Asn Glu Leu Asp Leu Asn Lys Glu Gln 245 250 255 Lys Gly Tyr Asp Pro Arg Lys Leu Leu Leu Pro Gly Thr Asp Ala Ile 260 265 270 Thr Ala Lys Met Lys Asp Met Ile Glu Trp Leu Gly Thr Pro Ala Val 275 280 285 Ala <210> 60 <211> 293 <212> PRT <213> Lactobacillus rhamnosus <400> 60 Met Pro Leu Val Asn Ala Ala Glu Leu Val Lys Ala Ala His Lys Gly 1 5 10 15 His Tyr Cys Ile Gly Ala Phe Asn Thr Asn Asn Leu Glu Trp Thr Arg 20 25 30 Ala Ile Leu Ala Gly Ala Gln Glu Leu Asn Val Pro Val Ile Ile Gln 35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Glu Phe Cys Gln 50 55 60 Thr Met Ile Glu Ala Ala Val Lys Ala Met Asp Ile Thr Val Pro Val 65 70 75 80 Val Ile His Leu Asp His Gly Asn Tyr Glu Ala Ala Lys Glu Ala Ile 85 90 95 Ala Ala Gly Tyr Asn Ser Val Met Phe Asp Gly His Asp Leu Asp Phe 100 105 110 Glu Asp Asn Leu Glu Lys Thr Lys Glu Ile Val Lys Leu Ala His Ala 115 120 125 Lys Gly Ile Ser Val Glu Ala Glu Val Gly Ser Ile Gly Gly Glu Glu 130 135 140 Asp Gly Val Val Gly Glu Gly Glu Leu Ala Asp Val Glu Glu Ala Lys 145 150 155 160 Thr Leu Ala Ala Thr Gly Ile Asp Phe Leu Ala Ala Gly Ile Gly Asn 165 170 175 Ile His Gly Gln Tyr Pro Asp Asn Trp Lys Gly Leu His Phe Asp Arg 180 185 190 Leu Gln Glu Leu Asn Asp Ala Val Lys Met Pro Leu Val Leu His Gly 195 200 205 Gly Ser Gly Ile Pro Gln Glu Gln Val Gln Lys Ala Ile Thr Met Gly 210 215 220 Ile Ser Lys Leu Asn Ile Asn Thr Glu Cys Gln Leu Ala Phe Ala Lys 225 230 235 240 Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Gln Gln Gly Lys Gly 245 250 255 Phe Asp Pro Arg Lys Met Leu Lys Pro Gly Thr Asp Ala Ile Thr Asp 260 265 270 Thr Phe Lys Glu Ile Thr Gly Trp Ile Gly Asn Lys Pro Val Lys Met 275 280 285 Val Pro Glu Ala Leu 290 <210> 61 <211> 288 <212> PRT <213> Lactobacillus ruminis <400> 61 Met Ser Phe Val Asn Gly Asn Ala Ile Phe Gly Ala Ala Arg Lys Gly 1 5 10 15 His Tyr Cys Val Gly Ala Phe Asn Thr Asn Asn Leu Glu Trp Thr Arg 20 25 30 Ala Ile Leu Lys Gly Ala Gln Glu Lys Asn Val Pro Val Leu Ile Gln 35 40 45 Val Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Leu Val Arg 50 55 60 Asp Leu Val Ala Ser Glu Met Glu Ala Met Asn Ile Thr Val Pro Val 65 70 75 80 Val Met His Leu Asp His Gly Asn Tyr Glu Ala Ala Lys Glu Cys Ile 85 90 95 Glu Ala Gly Tyr Ser Ser Val Met Phe Asp Gly His Asp Leu Pro Leu 100 105 110 Glu Glu Asn Leu Ala Lys Thr Lys Glu Ile Val Glu Leu Ala His Ala 115 120 125 His Gly Met Ser Val Glu Ala Glu Val Gly Ser Ile Gly Gly Thr Glu 130 135 140 Asp Gly Ile Thr Gly Ala Gly Glu Leu Ala Ser Val Glu Asp Ala Thr 145 150 155 160 Lys Met Ala Ala Thr Gly Val Asp Tyr Leu Ala Cys Gly Ile Gly Asn 165 170 175 Ile His Gly Lys Tyr Pro Ala Asp Trp Ala Gly Leu Asn Phe Asp Arg 180 185 190 Leu Lys Glu Ile Ala Asp Ala Leu Pro Asp Met Pro Leu Val Leu His 195 200 205 Gly Gly Ser Gly Ile Pro Gln Glu Gln Val Glu Lys Ala Ile Ser Leu 210 215 220 Gly Ile Ser Lys Leu Asn Ile Asn Thr Glu Cys Gln Leu Ala Phe Ala 225 230 235 240 Asp Ala Thr Arg Lys Tyr Ile Glu Ala Gly Lys Asp Gln Glu Gly Lys 245 250 255 Gly Tyr Asp Pro Arg Lys Leu Leu Ala Pro Gly Thr Gln Ala Ile Thr 260 265 270 Asp Thr Val Glu Glu Ile Ile Gly Trp Met Gly Thr Pro Ser Ile Asp 275 280 285 <210> 62 <211> 288 <212> PRT <213> Lactobacillus salivarius <400> 62 Met Ser Val Phe Val Asn Gly Asn Ala Ile Phe Asn Ala Ala Arg Gln 1 5 10 15 Gly His Tyr Ala Val Gly Ala Phe Asn Thr Asn Asn Leu Glu Trp Thr 20 25 30 Arg Ala Ile Leu Lys Ala Ala Gln Glu Thr Asn Thr Pro Val Leu Ile 35 40 45 Gln Val Ser Met Gly Ala Ala Lys Tyr Met Gly Asp Tyr Lys Leu Val 50 55 60 Lys Lys Leu Val Glu Glu Glu Ile Arg Val Met Asn Ile Thr Val Pro 65 70 75 80 Val Ile Met His Leu Asp His Gly Asn Tyr Glu Ala Ala Lys Glu Cys 85 90 95 Ile Glu Ala Gly Tyr Ser Ser Val Met Phe Asp Gly His Asp Leu Pro 100 105 110 Phe Glu Glu Asn Leu Glu Lys Thr Ala Glu Ile Val Lys Leu Ala His 115 120 125 Ala Lys Gly Ile Ser Val Glu Ala Glu Val Gly Ser Ile Gly Gly Glu 130 135 140 Glu Asp Gly Ile Val Gly Glu Gly Glu Leu Ala Ser Val Glu Glu Ala 145 150 155 160 Lys Gln Ile Ala Ala Leu Gly Val Asp Tyr Leu Ala Cys Gly Ile Gly 165 170 175 Asn Ile His Gly Pro Tyr Pro Glu Asn Trp Lys Gly Leu His Phe Asp 180 185 190 Arg Leu Gln Glu Ile Ala Asp Ala Ile Asp Thr Pro Leu Val Leu His 195 200 205 Gly Gly Ser Gly Ile Pro Gln Glu Gln Ile Glu Lys Ala Ile Lys Met 210 215 220 Gly Ile Ser Lys Val Asn Ile Asn Thr Glu Asn Gln Leu Ala Phe Ala 225 230 235 240 Ala Ala Thr Arg Lys Tyr Ile Glu Ala Gly Lys Asp Gln Glu Gly Lys 245 250 255 Gly Tyr Asp Pro Arg Lys Leu Leu Ala Pro Gly Ser Glu Ala Ile Val 260 265 270 Asp Thr Val Lys Glu Ile Ile Gly Trp Leu Gly Thr Pro Glu Val Lys 275 280 285 <210> 63 <211> 303 <212> PRT <213> Lactobacillus sp. <400> 63 Met Ala Tyr Phe Val Asn Gly Asn Glu Ile Phe Arg Glu Ala Arg Lys 1 5 10 15 His His Tyr Ala Val Gly Ala Tyr Asn Thr Asn Asn Leu Glu Trp Thr 20 25 30 Arg Ala Glu Leu Arg Ala Ala Glu Glu Thr Arg Thr Pro Leu Leu Ile 35 40 45 Gln Val Ser Thr Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Phe Val 50 55 60 Lys Asp Met Val Glu Asp Gln Met Asp Ala Met Asn Ile Ser Val Pro 65 70 75 80 Val Ile Leu Asn Leu Asp His Gly Asp Phe Glu Ser Ala Lys Glu Cys 85 90 95 Ile Ala Leu Gly Tyr Ser Ser Val Met Phe Asp Gly His Ala Leu Pro 100 105 110 Thr Glu Glu Asn Leu Ala Lys Thr Lys Glu Ile Ile Lys Leu Ala His 115 120 125 Glu Arg Gly Ile Ser Val Glu Ala Glu Ile Gly Lys Ile Gly Glu Asn 130 135 140 Gln Gly Gly Gly Glu Leu Ala Ser Val Glu Asp Ala Lys Ala Phe Val 145 150 155 160 Glu Ala Gly Val Asp Lys Leu Ala Cys Gly Ile Gly Asn Ile His Gly 165 170 175 Val Tyr Pro Ala Asp Trp Lys Gly Leu Asn Phe Asp Arg Leu Lys Glu 180 185 190 Ile Ala Glu Ala Val Pro Glu Thr Pro Leu Val Leu His Gly Gly Ser 195 200 205 Gly Ile Pro Glu Asp Gln Ile Lys Lys Ala Ile Gln Leu Gly Ile Ser 210 215 220 Lys Val Asn Ile Asn Thr Glu Phe Gln Leu Ala Phe Gln Glu Ala Thr 225 230 235 240 Arg Lys Tyr Ile Glu Glu Gly Lys Asp Glu Asp Lys Ala Asn Lys Gly 245 250 255 Tyr Asp Pro Arg Lys Leu Leu Leu Pro Gly Thr Glu Ala Ile Thr Asp 260 265 270 Lys Met Lys Glu Met Ile Ala Trp Leu Gly Thr Lys Thr Ile Asp Glu 275 280 285 Glu Ile Lys Asp Ala Ser Phe Asp Arg Ser Ser Leu Asn Glu Glu 290 295 300 <210> 64 <211> 354 <212> PRT <213> Marinobacter hydrocarbonoclasticus <400> 64 Met Ala Leu Ile Ser Leu Arg Gln Leu Leu Asp His Ala Ala Glu His 1 5 10 15 Gly Tyr Gly Val Pro Ala Phe Asn Val Asn Asn Leu Glu Gln Met Arg 20 25 30 Ala Ile Met Glu Ala Ala Asp Lys Thr Asp Ser Pro Val Ile Val Gln 35 40 45 Ala Ser Ala Gly Ala Arg Lys Tyr Ala Gly Ala Pro Phe Leu Arg His 50 55 60 Leu Ile Leu Ala Ala Ile Glu Glu Trp Pro His Ile Pro Val Val Met 65 70 75 80 His Gln Asp His Gly Thr Ser Pro Ala Val Cys Gln Arg Ser Ile Gln 85 90 95 Leu Gly Phe Ser Ser Val Met Met Asp Gly Ser Leu Gly Glu Asp Gly 100 105 110 Lys Thr Pro Thr Ser Tyr Glu Tyr Asn Val Asp Val Thr Arg Arg Thr 115 120 125 Val Glu Met Ala His Ala Cys Gly Val Ser Val Glu Gly Glu Leu Gly 130 135 140 Cys Leu Gly Ser Leu Glu Thr Gly Gln Ala Gly Glu Glu Asp Gly Ile 145 150 155 160 Gly Ala Glu Gly Thr Leu Asp His Ser Gln Met Leu Thr Asp Pro Glu 165 170 175 Glu Ala Ala Asp Phe Val Gln Lys Thr Gln Val Asp Ala Leu Ala Ile 180 185 190 Ala Ile Gly Thr Ser His Gly Ala Tyr Lys Phe Thr Arg Pro Pro Thr 195 200 205 Gly Asp Ile Leu Ala Ile Asp Gln Ile Lys Ala Ile His Ala Arg Ile 210 215 220 Pro Asp Thr His Leu Val Met His Gly Ser Ser Ser Val Pro Gln Glu 225 230 235 240 Trp Leu Lys Val Ile Asn Glu Phe Gly Gly Glu Ile Pro Glu Thr Tyr 245 250 255 Gly Val Pro Val Glu Glu Ile Val Glu Gly Ile Lys His Gly Val Arg 260 265 270 Lys Val Asn Ile Asp Thr Asp Leu Arg Leu Ala Ser Thr Gly Ala Val 275 280 285 Arg Arg Phe Met Ala Gln Asn Pro Ala Glu Phe Asp Pro Arg Lys Phe 290 295 300 Leu Lys Ala Thr Met Val Ala Met Thr Asp Ile Cys Val Ala Arg Tyr 305 310 315 320 Glu Ala Phe Gly Cys Ala Gly Gln Ala Ser Lys Ile Lys Pro Leu Asn 325 330 335 Leu Glu Gln Met Phe Glu Arg Tyr Ala Ala Gly Glu Leu Asp Pro Lys 340 345 350 Val Lys <210> 65 <211> 349 <212> PRT <213> Methylobacillus flagellatus <400> 65 Met Ala Leu Ile Ser Leu Arg Gln Leu Leu Asp His Ala Ala Glu His 1 5 10 15 Asp Tyr Gly Val Pro Ala Phe Asn Val Asn Asn Leu Glu Gln Val Gln 20 25 30 Ala Ile Met Arg Ala Ala Asp Ser Cys His Ser Pro Val Ile Leu Gln 35 40 45 Ala Ser Ala Gly Ala Arg Lys Tyr Ala Gly Glu Ala Phe Leu Leu Lys 50 55 60 Leu Ala Glu Ala Ala Ile Ala Ser Tyr Pro His Leu Pro Val Val Leu 65 70 75 80 His Gln Asp His Gly Ala Ser Pro Ala Ile Cys Leu His Ala Ile Gln 85 90 95 Ser Gly Phe Ser Ser Val Met Met Asp Gly Ser Leu Leu Glu Asp Gly 100 105 110 Lys Thr Pro Ala Ser Tyr Glu Tyr Asn Val Glu Val Thr Arg Gln Val 115 120 125 Val Met Ala Ala His Ala Val Gly Val Ser Val Glu Gly Glu Leu Gly 130 135 140 Val Leu Gly Ser Leu Glu Thr Gly Leu Ala Gly Glu Glu Asp Gly Val 145 150 155 160 Gly Ala Glu Gly Arg Leu Asp Arg Ser Gln Leu Leu Thr Ser Pro Glu 165 170 175 Glu Ala Val Asp Phe Val Ala Arg Thr Gly Val Asp Ala Leu Ala Val 180 185 190 Ala Ile Gly Thr Ser His Gly Ala Tyr Lys Phe Lys Arg Pro Pro Thr 195 200 205 Gly Glu Thr Leu Ala Ile Ser Arg Leu Arg Glu Ile His Ala Arg Leu 210 215 220 Pro Asn Thr His Leu Val Met His Gly Ser Ser Ser Val Pro Gln Glu 225 230 235 240 Trp Leu Gln Val Ile Asn Glu Tyr Gly Gly Glu Ile Pro Glu Thr Tyr 245 250 255 Gly Val Pro Val Glu Glu Ile Val Lys Gly Ile Arg Ser Gly Val Arg 260 265 270 Lys Val Asn Ile Asp Thr Asp Leu Arg Leu Ala Phe Thr Gly Ala Leu 275 280 285 Arg Arg Phe Leu Gly Gln Pro Glu His Thr Ala Glu Phe Asp Pro Arg 290 295 300 Lys Ile Leu Ala Ala Ala Thr Ser Ala Met Gln Thr Leu Cys Arg Glu 305 310 315 320 Arg Phe Glu Ala Phe Gly Ser Ala Gly Arg Ala Thr Gln Ile Gln Pro 325 330 335 Val Pro Leu Asp Glu Met Ala Gln Arg Tyr Val Val Ser 340 345 <210> 66 <211> 354 <212> PRT <213> Rhizobium leguminosarum bv. trifolii <400> 66 Met Ala Arg Ile Thr Leu Arg Gln Leu Leu Asp His Ala Ala Glu Glu 1 5 10 15 Gly Tyr Gly Val Pro Ala Phe Asn Ile Asn Asn Met Glu Gln Ala Leu 20 25 30 Ala Ile Met Glu Ala Ala Asp Ala Cys His Ala Pro Val Ile Met Gln 35 40 45 Ala Ser Arg Gly Ala Arg Ala Tyr Ala His Asp Ile Met Leu Lys His 50 55 60 Met Met Asp Ala Val Val Glu Ile Tyr Pro His Ile Pro Val Cys Val 65 70 75 80 His Leu Asp His Gly Asn Asp Pro Ser Asn Cys Met Thr Ala Ile Gln 85 90 95 Ala Gly Phe Thr Ser Val Met Met Asp Gly Ser Leu Lys Ala Asp Ala 100 105 110 Lys Thr Pro Ala Asp Trp Ala Tyr Asn Val Gly Val Thr Lys Met Val 115 120 125 Thr Asp Met Ala His Phe Gly Gly Ile Ser Val Glu Gly Glu Leu Gly 130 135 140 Val Leu Gly Ser Leu Glu Thr Gly Met Gly Glu Ala Glu Asp Gly His 145 150 155 160 Gly Ala Glu Gly Lys Leu Ser His Asp Gln Leu Leu Thr Asp Pro Asp 165 170 175 Glu Ala Val Lys Phe Val Arg Glu Thr Lys Val Asp Ala Leu Ala Ile 180 185 190 Ala Met Gly Thr Ser His Gly Ala Tyr Lys Phe Thr Arg Lys Pro Asp 195 200 205 Gly Ser Val Leu Ala Met Asn Val Ile Glu Glu Ile His Arg Lys Leu 210 215 220 Pro Asn Thr His Leu Val Met His Gly Ser Ser Ser Val Pro Ile Glu 225 230 235 240 Leu Gln Glu Ile Ile Asn Lys Tyr Gly Gly Gln Met Lys Pro Thr Trp 245 250 255 Gly Val Pro Val Glu Glu Ile Gln Arg Gly Ile Lys Asn Gly Val Arg 260 265 270 Lys Val Asn Ile Asp Thr Asp Gly Arg Met Ala Met Thr Gly Gln Ile 275 280 285 Arg Arg Val Leu Gln Glu Asp Pro Ser Glu Phe Asp Pro Arg Lys Tyr 290 295 300 Leu Lys Pro Ala Met Thr Ala Leu Thr Lys Leu Cys Lys Glu Arg Phe 305 310 315 320 Glu Gln Phe Gly Thr Ala Gly Met Ala Gly Arg Ile Thr Pro Leu Pro 325 330 335 Val Ser Glu Met Ala Lys Arg Tyr Lys Ser Gly Ser Leu Asp Pro Ala 340 345 350 Phe Ser <210> 67 <211> 354 <212> PRT <213> Rhizobium lupini <400> 67 Met Ala Arg Ile Thr Leu Arg Gln Leu Leu Asp His Ala Ala Glu Glu 1 5 10 15 Gly Tyr Gly Val Pro Ala Phe Asn Ile Asn Asn Met Glu Gln Ala Leu 20 25 30 Ala Ile Met Glu Ala Ala Asp Ala Cys His Ala Pro Val Ile Met Gln 35 40 45 Ala Ser Arg Gly Ala Arg Ala Tyr Ala His Asp Ile Met Leu Lys His 50 55 60 Met Met Asp Ala Val Val Glu Ile Tyr Pro His Ile Pro Val Cys Val 65 70 75 80 His Leu Asp His Gly Asn Asp Pro Ser Asn Cys Met Thr Ala Ile Gln 85 90 95 Ala Gly Phe Thr Ser Val Met Met Asp Gly Ser Leu Lys Ala Asp Ala 100 105 110 Lys Thr Pro Ala Asp Trp Ala Tyr Asn Val Gly Val Thr Lys Met Val 115 120 125 Thr Asp Met Ala His Phe Gly Gly Ile Ser Val Glu Gly Glu Leu Gly 130 135 140 Val Leu Gly Ser Leu Glu Thr Gly Met Gly Glu Ala Glu Asp Gly His 145 150 155 160 Gly Ala Glu Gly Lys Leu Ser His Asp Gln Leu Leu Thr Asp Pro Asp 165 170 175 Glu Ala Val Lys Phe Val Arg Glu Thr Lys Val Asp Ala Leu Ala Ile 180 185 190 Ala Met Gly Thr Ser His Gly Ala Tyr Lys Phe Thr Arg Lys Pro Asp 195 200 205 Gly Ser Val Leu Ala Met Asn Val Ile Glu Glu Ile His Arg Lys Leu 210 215 220 Pro Asn Thr His Leu Val Met His Gly Ser Ser Ser Val Pro Ile Glu 225 230 235 240 Leu Gln Glu Ile Ile Asn Lys Tyr Gly Gly Gln Met Lys Pro Thr Trp 245 250 255 Gly Val Pro Val Glu Glu Ile Gln Arg Gly Ile Lys Asn Gly Val Arg 260 265 270 Lys Val Asn Ile Asp Thr Asp Gly Arg Met Ala Met Thr Gly Gln Ile 275 280 285 Arg Arg Val Leu Gln Glu Asp Pro Ser Glu Phe Asp Pro Arg Lys Tyr 290 295 300 Leu Lys Pro Ala Met Thr Ala Leu Thr Lys Leu Cys Lys Glu Arg Phe 305 310 315 320 Glu Gln Phe Gly Thr Ala Gly Met Ala Gly Arg Ile Thr Pro Leu Pro 325 330 335 Val Ser Glu Met Ala Lys Arg Tyr Lys Ser Gly Ser Leu Asp Pro Ala 340 345 350 Phe Ser <210> 68 <211> 354 <212> PRT <213> Rhizobium sp. <400> 68 Met Ala Arg Ile Thr Leu Arg Gln Leu Leu Asp His Ala Ala Glu Glu 1 5 10 15 Gly Tyr Gly Val Pro Ala Phe Asn Ile Asn Asn Met Glu Gln Ala Leu 20 25 30 Ala Ile Met Glu Ala Ala Asp Ala Cys Gln Ala Pro Val Ile Met Gln 35 40 45 Ala Ser Arg Gly Ala Arg Ala Tyr Ala Asn Asp Ile Met Leu Lys His 50 55 60 Met Met Asp Ala Val Val Glu Ile Tyr Pro His Ile Pro Val Cys Val 65 70 75 80 His Leu Asp His Gly Asn Asp Pro Ser Asn Cys Met Thr Ala Ile Gln 85 90 95 Ala Gly Phe Thr Ser Val Met Met Asp Gly Ser Leu Lys Ala Asp Ala 100 105 110 Lys Thr Pro Ala Asp Trp Ala Tyr Asn Val Gly Val Thr Lys Met Val 115 120 125 Thr Asp Met Ala His Phe Gly Gly Ile Ser Val Glu Gly Glu Leu Gly 130 135 140 Val Leu Gly Ser Leu Glu Thr Gly Met Gly Glu Ala Glu Asp Gly His 145 150 155 160 Gly Ala Glu Gly Lys Leu Ser His Asp Gln Leu Leu Thr Asp Pro Asp 165 170 175 Glu Ala Val Lys Phe Val Arg Glu Thr Lys Val Asp Ala Leu Ala Ile 180 185 190 Ala Met Gly Thr Ser His Gly Ala Tyr Lys Phe Thr Arg Lys Pro Asp 195 200 205 Gly Ser Val Leu Ala Met Asn Val Ile Glu Glu Ile His Arg Lys Leu 210 215 220 Pro Asn Thr His Leu Val Met His Gly Ser Ser Ser Val Pro Ile Glu 225 230 235 240 Leu Gln Glu Ile Ile Asn Lys Tyr Gly Gly Gln Met Lys Pro Thr Trp 245 250 255 Gly Val Pro Val Glu Glu Ile Gln Arg Gly Ile Lys Asn Gly Val Arg 260 265 270 Lys Val Asn Ile Asp Thr Asp Gly Arg Met Ala Met Thr Gly Gln Ile 275 280 285 Arg Arg Val Leu Gln Glu Asp Pro Ser Glu Phe Asp Pro Arg Lys Tyr 290 295 300 Leu Lys Pro Ala Met Thr Ala Leu Thr Lys Leu Cys Lys Glu Arg Phe 305 310 315 320 Glu Gln Phe Gly Thr Ala Gly Met Ala Gly Arg Ile Thr Pro Leu Ala 325 330 335 Val Ser Glu Met Ala Lys Arg Tyr Lys Ser Gly Ser Leu Asp Pro Ala 340 345 350 Phe Ser <210> 69 <211> 286 <212> PRT <213> Staphylococcus epidermidis <400> 69 Met Pro Leu Val Ser Met Lys Glu Met Leu Ile Asp Ala Lys Glu Asn 1 5 10 15 Gly Tyr Ala Val Gly Gln Tyr Asn Leu Asn Asn Leu Glu Phe Thr Gln 20 25 30 Ala Ile Leu Glu Ala Ser Gln Glu Glu Asn Ala Pro Val Ile Leu Gly 35 40 45 Val Ser Glu Gly Ala Ala Arg Tyr Met Ser Gly Phe Tyr Thr Val Val 50 55 60 Lys Met Val Glu Gly Leu Met His Asp Leu Asn Ile Thr Ile Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Lys Cys Lys Glu Ala 85 90 95 Ile Asp Ala Gly Phe Thr Ser Val Met Ile Asp Ala Ser His Ser Pro 100 105 110 Phe Glu Glu Asn Val Glu Ile Thr Ser Lys Val Val Glu Tyr Ala His 115 120 125 Asp Arg Gly Val Ser Val Glu Ala Glu Leu Gly Thr Val Gly Gly Gln 130 135 140 Glu Asp Asp Val Val Ala Asp Gly Val Ile Tyr Ala Asp Pro Lys Glu 145 150 155 160 Cys Gln Glu Leu Val Glu Lys Thr Gly Ile Asp Thr Leu Ala Pro Ala 165 170 175 Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Lys Leu Gly Phe 180 185 190 Lys Glu Met Glu Glu Ile Gly Ala Ser Thr Gly Leu Pro Leu Val Leu 195 200 205 His Gly Gly Thr Gly Ile Pro Thr Lys Asp Ile Gln Lys Ala Ile Pro 210 215 220 Tyr Gly Thr Ala Lys Ile Asn Val Asn Thr Glu Asn Gln Ile Ala Ser 225 230 235 240 Ala Lys Ala Val Arg Glu Val Leu Asn Asn Asp Lys Asp Val Tyr Asp 245 250 255 Pro Arg Lys Tyr Leu Gly Pro Ala Arg Glu Ala Ile Lys Glu Thr Val 260 265 270 Lys Gly Lys Ile Arg Glu Phe Gly Thr Ser Asn Arg Ala Lys 275 280 285 <210> 70 <211> 293 <212> PRT <213> Streptococcus thermophilus <400> 70 Met Ala Ile Val Ser Ala Glu Lys Phe Val Gln Ser Ala Arg Asp Asn 1 5 10 15 Gly Tyr Ala Leu Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Gln 20 25 30 Ala Ile Leu Arg Ala Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln 35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Leu Cys Lys 50 55 60 Ala Leu Ile Glu Glu Leu Val Glu Ser Met Gly Ile Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly His Tyr Asp Asp Ala Leu Glu Cys Ile 85 90 95 Glu Val Gly Tyr Thr Ser Ile Met Phe Asp Gly Ser His Leu Pro Ile 100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Glu Val Val Glu Lys Ala His Ala 115 120 125 Lys Gly Ile Ser Val Glu Ala Glu Val Gly Thr Ile Gly Gly Glu Glu 130 135 140 Asp Gly Ile Val Gly Arg Gly Glu Leu Ala Pro Ile Glu Asp Ala Lys 145 150 155 160 Ala Met Val Ala Thr Gly Val Asp Phe Leu Ala Ala Gly Ile Gly Asn 165 170 175 Ile His Gly Pro Tyr Pro Glu Asn Trp Glu Gly Leu Asp Leu Asp His 180 185 190 Leu Gln Lys Leu Thr Glu Ala Ile Pro Gly Phe Pro Ile Val Leu His 195 200 205 Gly Gly Ser Gly Ile Pro Asp Asp Gln Ile Gln Glu Ala Ile Lys Leu 210 215 220 Gly Val Ala Lys Val Asn Val Asn Thr Glu Cys Gln Ile Ala Phe Ala 225 230 235 240 Asn Ala Thr Arg Lys Phe Val Ala Glu Tyr Glu Ala Asn Glu Ala Glu 245 250 255 Tyr Asp Lys Lys Lys Leu Phe Asp Pro Arg Lys Phe Leu Lys Pro Gly 260 265 270 Phe Glu Ala Ile Thr Glu Ala Val Glu Glu Arg Ile Asp Val Phe Gly 275 280 285 Ser Ala Asn Lys Ala 290 <210> 71 <211> 340 <212> PRT <213> Streptomyces avermitilis <400> 71 Met Pro Ile Ala Thr Pro Glu Val Tyr Asn Glu Met Leu Asp Arg Ala 1 5 10 15 Lys Ala Gly Lys Phe Ala Tyr Pro Ala Ile Asn Val Thr Ser Thr Gln 20 25 30 Thr Leu His Ala Ala Leu Arg Gly Phe Ala Glu Ala Glu Ser Asp Gly 35 40 45 Ile Val Gln Ile Ser Thr Gly Gly Ala Glu Phe Leu Gly Gly Gln Tyr 50 55 60 Lys Lys Asp Met Val Thr Gly Ala Val Ala Leu Ala Glu Phe Ala His 65 70 75 80 Ile Val Ala Glu Lys Tyr Pro Val Thr Val Ala Leu His Thr Asp His 85 90 95 Cys Pro Lys Asp Lys Leu Asp Gly Tyr Val Arg Pro Leu Leu Ala Ile 100 105 110 Ser Glu Glu Arg Val Lys Ala Gly Arg Asn Pro Leu Phe Gln Ser His 115 120 125 Met Trp Asp Gly Ser Ala Glu Thr Leu Ala Asp Asn Leu Ala Ile Ala 130 135 140 Gln Glu Leu Leu Glu Lys Ala Arg Ala Ala Lys Ile Ile Leu Glu Val 145 150 155 160 Glu Ile Thr Pro Thr Gly Gly Glu Glu Asp Gly Val Thr His Glu Ile 165 170 175 Asn Asp Ser Leu Tyr Thr Thr Val Glu Asp Ala Ile Arg Thr Ala Glu 180 185 190 Ala Leu Gly Leu Gly Glu Lys Gly Arg Tyr Leu Leu Ala Ala Ser Phe 195 200 205 Gly Asn Val His Gly Val Tyr Lys Pro Gly Asn Val Val Leu Arg Pro 210 215 220 Glu Leu Leu Lys Glu Leu Asn Glu Gly Val Ala Ala Lys Phe Gly Lys 225 230 235 240 Ala Asp Pro Phe Asp Phe Val Phe His Gly Gly Ser Gly Ser Thr Glu 245 250 255 Ala Glu Ile Arg Thr Ala Leu Glu Asn Gly Val Val Lys Met Asn Ile 260 265 270 Asp Thr Asp Thr Gln Tyr Ala Phe Thr Arg Pro Val Ala Asp His Met 275 280 285 Phe Arg Asn Tyr Asp Gly Val Leu Lys Val Asp Gly Glu Val Gly Asp 290 295 300 Lys Lys Thr Tyr Asp Pro Arg Thr Trp Gly Lys Leu Ala Glu Ala Ser 305 310 315 320 Met Ala Lys Arg Val Thr Glu Ala Cys Ala Asn Leu Arg Ser Thr Gly 325 330 335 Thr Lys Ile Lys 340 <210> 72 <211> 294 <212> PRT <213> Streptomyces viridochromogenes <400> 72 Met Thr Ile Asp Pro Gly Asp Thr Val Pro Leu Thr Thr Thr Gly Glu 1 5 10 15 Leu Val Thr Arg Ala Ala Ala Ala Arg Ala Ala Val Ala Ser Phe Asn 20 25 30 Val Ile Thr Leu Glu His Val Glu Ala Val Ile Ala Gly Ala Glu Ser 35 40 45 Val Pro Ala Pro Val Val Leu Gln Val Ser Glu Asn Ala Val Arg Phe 50 55 60 Arg Gly Gly Arg Leu Leu Pro Leu Ala Arg Ala Ala Val Thr Ala Ala 65 70 75 80 Glu His Ala Asp Val Pro Val Ala Leu His Leu Asp His Val Gln Ser 85 90 95 Glu Ala Leu Leu His Gln Ala Ala Asp Val Gly Phe Ser Ser Val Met 100 105 110 Tyr Asp Ala Ala Arg Leu Pro Tyr Ala Glu Asn Leu Ala Ala Thr Arg 115 120 125 Ala Ala Val Asp Trp Ala His Ala Arg Gly Leu Trp Ile Glu Ala Glu 130 135 140 Leu Gly Gln Ile Gly Gly Lys Asn Gly Arg Pro Pro Leu Asp Ala His 145 150 155 160 Ala Pro Gly Ala Arg Thr Asp Pro Asp Glu Ala Arg Asp Phe Val Ala 165 170 175 Asp Ser Gly Val Asp Ala Leu Ala Val Ala Ile Gly Ser Ala His Ala 180 185 190 Met Thr Thr Arg Thr Ala Ala Leu Asp His Ala Leu Leu Lys Gln Leu 195 200 205 Ser Ala Ala Leu Asp Val Pro Leu Val Leu His Gly Ser Ser Gly Val 210 215 220 Pro Asp Gly Glu Leu Thr Ala Ala Val Ala Gly Gly Ile Thr Lys Val 225 230 235 240 Asn Ile Gly Thr Ala Leu Asn Val Ala Leu Thr Gly Ala Ile Arg Asp 245 250 255 Phe Leu Ala Asp Arg Pro Glu Ala Val Asp Pro Arg Gly Tyr Leu Thr 260 265 270 Val Ala Arg Glu Ala Met Thr Arg Ala Val Ala Arg Ala Leu Gln Val 275 280 285 Leu Gly Ala Thr Ile Ala 290 <210> 73 <211> 299 <212> PRT <213> Bacillus subtilis <400> 73 Met Leu Gly Gly Ile Glu Ala Gly Gly Thr Lys Phe Val Cys Ala Val 1 5 10 15 Gly Arg Glu Asp Gly Thr Ile Ile Asp Arg Ile Glu Phe Pro Thr Lys 20 25 30 Met Pro Asp Glu Thr Ile Glu Lys Val Ile Gln Tyr Phe Ser Gln Phe 35 40 45 Ser Leu Gln Ala Ile Gly Ile Gly Ser Phe Gly Pro Val Asp Asn Asp 50 55 60 Lys Thr Ser Gln Thr Tyr Gly Thr Ile Thr Ala Thr Pro Lys Ala Gly 65 70 75 80 Trp Arg His Tyr Pro Phe Leu Gln Thr Val Lys Asn Glu Met Lys Ile 85 90 95 Pro Val Gly Phe Ser Thr Asp Val Asn Ala Ala Ala Leu Gly Glu Phe 100 105 110 Leu Phe Gly Glu Ala Lys Gly Leu Asp Ser Cys Leu Tyr Ile Thr Ile 115 120 125 Gly Thr Gly Ile Gly Ala Gly Ala Ile Val Glu Gly Arg Leu Leu Gln 130 135 140 Gly Leu Ser His Pro Glu Met Gly His Ile Tyr Ile Arg Arg His Pro 145 150 155 160 Asp Asp Val Tyr Gln Gly Lys Cys Pro Tyr His Gly Asp Cys Phe Glu 165 170 175 Gly Leu Ala Ser Gly Pro Ala Ile Glu Ala Arg Trp Gly Lys Lys Ala 180 185 190 Ala Asp Leu Ser Asp Ile Ala Gln Val Trp Glu Leu Glu Gly Tyr Tyr 195 200 205 Ile Ala Gln Ala Leu Ala Gln Tyr Ile Leu Ile Leu Ala Pro Lys Lys 210 215 220 Ile Ile Leu Gly Gly Gly Val Met Gln Gln Lys Gln Val Phe Ser Tyr 225 230 235 240 Ile Tyr Gln Tyr Val Pro Lys Ile Met Asn Ser Tyr Leu Asp Phe Ser 245 250 255 Glu Leu Ser Asp Asp Ile Ser Asp Tyr Ile Val Pro Pro Arg Leu Gly 260 265 270 Ser Asn Ala Gly Ile Ile Gly Thr Leu Val Leu Ala His Gln Ala Leu 275 280 285 Gln Ala Glu Ala Ala Ser Gly Glu Val Arg Ser 290 295 <210> 74 <211> 307 <212> PRT <213> Escherichia coli <400> 74 Met Ser Ala Lys Val Trp Val Leu Gly Asp Ala Val Val Asp Leu Leu 1 5 10 15 Pro Glu Ser Asp Gly Arg Leu Leu Pro Cys Pro Gly Gly Ala Pro Ala 20 25 30 Asn Val Ala Val Gly Ile Ala Arg Leu Gly Gly Thr Ser Gly Phe Ile 35 40 45 Gly Arg Val Gly Asp Asp Pro Phe Gly Ala Leu Met Gln Arg Thr Leu 50 55 60 Leu Thr Glu Gly Val Asp Ile Thr Tyr Leu Lys Gln Asp Glu Trp His 65 70 75 80 Arg Thr Ser Thr Val Leu Val Asp Leu Asn Asp Gln Gly Glu Arg Ser 85 90 95 Phe Thr Phe Met Val Arg Pro Ser Ala Asp Leu Phe Leu Glu Thr Thr 100 105 110 Asp Leu Pro Cys Trp Arg His Gly Glu Trp Leu His Leu Cys Ser Ile 115 120 125 Ala Leu Ser Ala Glu Pro Ser Arg Thr Ser Ala Phe Thr Ala Met Thr 130 135 140 Ala Ile Arg His Ala Gly Gly Phe Val Ser Phe Asp Pro Asn Ile Arg 145 150 155 160 Glu Asp Leu Trp Gln Asp Glu His Leu Leu Arg Leu Cys Leu Arg Gln 165 170 175 Ala Leu Gln Leu Ala Asp Val Val Lys Leu Ser Glu Glu Glu Trp Arg 180 185 190 Leu Ile Ser Gly Lys Thr Gln Asn Asp Arg Asp Ile Cys Ala Leu Ala 195 200 205 Lys Glu Tyr Glu Ile Ala Met Leu Leu Val Thr Lys Gly Ala Glu Gly 210 215 220 Val Val Val Cys Tyr Arg Gly Gln Val His His Phe Ala Gly Met Ser 225 230 235 240 Val Asn Cys Val Asp Ser Thr Gly Ala Gly Asp Ala Phe Val Ala Gly 245 250 255 Leu Leu Thr Gly Leu Ser Ser Thr Gly Leu Ser Thr Asp Glu Arg Glu 260 265 270 Met Arg Arg Ile Ile Asp Leu Ala Gln Arg Cys Gly Ala Leu Ala Val 275 280 285 Thr Ala Lys Gly Ala Met Thr Ala Leu Pro Cys Arg Gln Glu Leu Glu 290 295 300 Ser Glu Lys 305 <210> 75 <211> 302 <212> PRT <213> Escherichia coli <400> 75 Met Arg Ile Gly Ile Asp Leu Gly Gly Thr Lys Thr Glu Val Ile Ala 1 5 10 15 Leu Gly Asp Ala Gly Glu Gln Leu Tyr Arg His Arg Leu Pro Thr Pro 20 25 30 Arg Asp Asp Tyr Arg Gln Thr Ile Glu Thr Ile Ala Thr Leu Val Asp 35 40 45 Met Ala Glu Gln Ala Thr Gly Gln Arg Gly Thr Val Gly Met Gly Ile 50 55 60 Pro Gly Ser Ile Ser Pro Tyr Thr Gly Val Val Lys Asn Ala Asn Ser 65 70 75 80 Thr Trp Leu Asn Gly Gln Pro Phe Asp Lys Asp Leu Ser Ala Arg Leu 85 90 95 Gln Arg Glu Val Arg Leu Ala Asn Asp Ala Asn Cys Leu Ala Val Ser 100 105 110 Glu Ala Val Asp Gly Ala Ala Ala Gly Ala Gln Thr Val Phe Ala Val 115 120 125 Ile Ile Gly Thr Gly Cys Gly Ala Gly Val Ala Phe Asn Gly Arg Ala 130 135 140 His Ile Gly Gly Asn Gly Thr Ala Gly Glu Trp Gly His Asn Pro Leu 145 150 155 160 Pro Trp Met Asp Glu Asp Glu Leu Arg Tyr Arg Glu Glu Val Pro Cys 165 170 175 Tyr Cys Gly Lys Gln Gly Cys Ile Glu Thr Phe Ile Ser Gly Thr Gly 180 185 190 Phe Ala Met Asp Tyr Arg Arg Leu Ser Gly His Ala Leu Lys Gly Ser 195 200 205 Glu Ile Ile Arg Leu Val Glu Glu Ser Asp Pro Val Ala Glu Leu Ala 210 215 220 Leu Arg Arg Tyr Glu Leu Arg Leu Ala Lys Ser Leu Ala His Val Val 225 230 235 240 Asn Ile Leu Asp Pro Asp Val Ile Val Leu Gly Gly Gly Met Ser Asn 245 250 255 Val Asp Arg Leu Tyr Gln Thr Val Gly Gln Leu Ile Lys Gln Phe Val 260 265 270 Phe Gly Gly Glu Cys Glu Thr Pro Val Arg Lys Ala Lys His Gly Asp 275 280 285 Ser Ser Gly Val Arg Gly Ala Ala Trp Leu Trp Pro Gln Glu 290 295 300 <210> 76 <211> 291 <212> PRT <213> Fusobacterium mortiferum <400> 76 Met Lys Phe Gly Ala Ile Asp Ala Gly Gly Lys Lys Phe Ile Cys Gly 1 5 10 15 Ile Thr Asp Glu Gln Gly Asn Thr Leu Glu Lys Thr Ser Phe Leu Thr 20 25 30 Glu Thr Pro Glu Lys Thr Ile Pro Leu Val Ile Asp Phe Phe Arg Asn 35 40 45 Lys Asp Ile Ser Ala Leu Gly Val Gly Cys Phe Gly Pro Leu Asp Leu 50 55 60 Asn Pro Asn Ser Asp Thr Tyr Gly Tyr Ile Thr Ser Ser Pro Lys Thr 65 70 75 80 Asp Trp Lys Asn Tyr Asn Ile Leu Gly Asn Leu Lys Ser Ala Phe Asn 85 90 95 Ile Pro Ile Phe Leu Asp Thr Asn Met Asn Val Gly Val Leu Gly Glu 100 105 110 Ala Met Trp Gly Ala Gly Lys Gly Leu Lys Asn Thr Ile Phe Leu Thr 115 120 125 Ile Gly Asn Gly Ile Gly Gly Gly Ala Ile Val Glu Gly Lys Ile Val 130 135 140 His Gly Met Leu His Pro Glu Met Gly His Met Phe Val Ser Arg His 145 150 155 160 Pro Arg Asp Lys Phe Ala Gly Thr Cys Pro Phe His Gly Gly Asn Cys 165 170 175 Leu Glu Gly Met Ala Ser Val Pro Ala Ile Glu Lys Arg Trp Gly Lys 180 185 190 Ser Leu Ser Ser Leu Pro Ile Glu His Pro Ala Trp Asp Leu Glu Ala 195 200 205 Phe Tyr Ile Ala His Ala Leu Val Asn Tyr Ile Leu Ile Leu Ser Pro 210 215 220 Glu Lys Ile Ile Leu Gly Gly Glu Val Met Lys Tyr Lys Gln Leu Leu 225 230 235 240 Pro Ile Val Lys Lys Ala Val Ile Lys Leu Leu Asn Gly Tyr Ile Gln 245 250 255 Thr Asn Gln Ile Phe Arg Glu Ile Asp Asp Tyr Ile Val Leu Pro Asp 260 265 270 Leu Gly Glu Asn Ser Gly Phe Phe Gly Ala Ala Ala Leu Cys Ile Lys 275 280 285 Asn Phe Lys 290 <210> 77 <211> 307 <212> PRT <213> Klebsiella pneumoniae <400> 77 Met Asn Gly Lys Ile Trp Val Leu Gly Asp Ala Val Val Asp Leu Leu 1 5 10 15 Pro Asp Gly Glu Gly Arg Leu Leu Gln Cys Pro Gly Gly Ala Pro Ala 20 25 30 Asn Val Ala Val Gly Val Ala Arg Leu Gly Gly Asp Ser Gly Phe Ile 35 40 45 Gly Arg Val Gly Asp Asp Pro Phe Gly Arg Phe Met Arg His Thr Leu 50 55 60 Ala Gln Glu Gln Val Asp Val Asn Tyr Met Arg Leu Asp Ala Ala Gln 65 70 75 80 Arg Thr Ser Thr Val Val Val Asp Leu Asp Ser His Gly Glu Arg Thr 85 90 95 Phe Thr Phe Met Val Arg Pro Ser Ala Asp Leu Phe Leu Gln Pro Glu 100 105 110 Asp Leu Pro Pro Phe Ala Ala Gly Gln Trp Leu His Val Cys Ser Ile 115 120 125 Ala Leu Ser Ala Glu Pro Ser Arg Ser Thr Thr Phe Ala Ala Leu Glu 130 135 140 Ala Ile Lys Arg Ala Gly Gly Tyr Val Ser Phe Asp Pro Asn Ile Arg 145 150 155 160 Ser Asp Leu Trp Gln Asp Pro Gln Asp Leu Arg Asp Cys Leu Asp Arg 165 170 175 Ala Leu Ala Leu Ala Asp Ala Ile Lys Leu Ser Glu Glu Glu Leu Ala 180 185 190 Phe Ile Ser Gly Ser Asp Asp Ile Val Ser Gly Ile Ala Arg Leu Asn 195 200 205 Ala Arg Phe Gln Pro Thr Leu Leu Leu Val Thr Gln Gly Lys Ala Gly 210 215 220 Val Gln Ala Ala Leu Arg Gly Gln Val Ser His Phe Pro Ala Arg Pro 225 230 235 240 Val Val Ala Val Asp Thr Thr Gly Ala Gly Asp Ala Phe Val Ala Gly 245 250 255 Leu Leu Ala Gly Leu Ala Ala His Gly Ile Pro Asp Asn Leu Ala Ala 260 265 270 Leu Ala Pro Asp Leu Ala Leu Ala Gln Thr Cys Gly Ala Leu Ala Thr 275 280 285 Thr Ala Lys Gly Ala Met Thr Ala Leu Pro Tyr Lys Asp Asp Leu Gln 290 295 300 Arg Ser Leu 305 <210> 78 <211> 290 <212> PRT <213> Lactococcus lactis subsp. cremoris <400> 78 Met Ser Val Tyr Tyr Gly Ser Ile Glu Ala Gly Gly Thr Lys Phe Val 1 5 10 15 Leu Ala Ile Ala Asp Glu His Phe Asn Ile Ile Lys Lys Phe Lys Phe 20 25 30 Ala Thr Thr Thr Pro Gln Glu Thr Ile Ser Lys Thr Ile Lys Tyr Phe 35 40 45 Lys Glu Asn Arg Val Ser Ala Ile Gly Leu Gly Ser Phe Gly Pro Ile 50 55 60 Asp Leu Asn Leu Ser Ser Lys Thr Tyr Gly Tyr Ile Thr Ser Thr Pro 65 70 75 80 Lys Val Gly Trp Lys Asn Ile Asn Leu Val Gly Gln Leu Lys Glu Ala 85 90 95 Leu Asp Ile Pro Ile Tyr Phe Thr Thr Asp Val Asn Ala Ser Ala Tyr 100 105 110 Gly Glu Met Lys Asn Thr Gly Ile Lys Asn Leu Val Tyr Leu Thr Ile 115 120 125 Gly Thr Gly Ile Gly Gly Gly Ala Ile Gln Asn Gly Tyr Phe Ile Gly 130 135 140 Gly Ile Gly His Ser Glu Met Gly His Gln Arg Ile Asn Arg His Arg 145 150 155 160 Asp Val Leu Thr Phe Glu Gly Ile Cys Pro Phe His Gly Asp Cys Leu 165 170 175 Glu Gly Val Ala Ala Gly Pro Ser Leu Glu Ala Arg Thr Gly Ile Leu 180 185 190 Gly Glu Lys Ile Ser Ser Asp Asp Pro Ile Trp Asp Ile Leu Ser Tyr 195 200 205 Tyr Ile Ala Gln Ala Ala Ile Asn Ala Thr Leu Thr Leu Ala Pro Glu 210 215 220 Cys Ile Ile Leu Gly Gly Gly Val Met Glu Lys Pro Asn Met Ile Ser 225 230 235 240 Leu Ile Gln Lys Gln Phe Ile Ser Met Leu Asn Asn Tyr Ile Asp Leu 245 250 255 Pro Cys Ser Val Glu Lys Tyr Ile Arg Leu Pro Thr Val Lys Glu Asn 260 265 270 Gly Ser Ala Thr Leu Gly Asn Phe Tyr Leu Ala Tyr Ser Leu Phe Thr 275 280 285 Lys Glu 290 <210> 79 <211> 291 <212> PRT <213> Lactococcus lactis subsp. lactis <400> 79 Met Thr Asn His Tyr Gly Ser Ile Glu Ala Gly Gly Thr Lys Phe Val 1 5 10 15 Leu Ala Ile Gly Asp Glu Gln Phe Asn Ile Leu Lys Thr Asn Gln Ile 20 25 30 Pro Thr Thr Ser Pro Glu Glu Thr Ile Thr Arg Thr Ile Glu Phe Phe 35 40 45 Lys Glu Asn Pro Val Lys Ser Leu Ala Val Gly Ser Phe Gly Pro Ile 50 55 60 Asp Ile Lys Pro Glu Ser Ala Thr Tyr Gly Tyr Ile Thr Thr Thr Pro 65 70 75 80 Lys Ala Gly Trp Ala Asn Val Asp Leu Leu Gly Lys Leu Lys Ala Ala 85 90 95 Leu Asp Ile Pro Met Val Phe Thr Thr Asp Val Asn Ser Ser Ala Tyr 100 105 110 Gly Glu Met Ile Val Thr Gly Gly Lys Ser Leu Val Tyr Phe Thr Ile 115 120 125 Gly Thr Gly Ile Gly Gly Gly Ala Val Gln Asp Gly Lys Phe Ile Gly 130 135 140 Gly Phe Ser His Ala Glu Met Gly His Gln Tyr Val Lys Arg His Pro 145 150 155 160 Asp Asp Leu Asn Phe Ala Gly Val Cys Pro Phe His Gly Asp Cys Leu 165 170 175 Glu Gly Val Ala Ala Gly Pro Ser Leu Glu Ala Arg Leu Gly Ile Arg 180 185 190 Gly Glu Asp Ile Pro Ala Asp Ser Lys Val Trp Asp Ile Gln Ala Phe 195 200 205 Tyr Ile Ala Gln Ile Ala Val Asn Thr Thr Leu Ala Leu Ala Pro Glu 210 215 220 Lys Ile Val Phe Gly Gly Gly Val Met Thr Gln Thr His Met Ile Glu 225 230 235 240 Arg Val Arg Lys Glu Phe Thr Lys Leu Met Ala Gly Tyr Val His Val 245 250 255 Pro Tyr Leu Leu Thr Asp Tyr Ile Met Thr Pro Ser Val Ala Asn Asn 260 265 270 Gly Ser Ala Thr Ile Gly Asn Phe Ala Leu Ala Lys Asp Leu Asp Glu 275 280 285 Lys Asn Tyr 290 <210> 80 <211> 288 <212> PRT <213> Pediococcus pentosaceus <400> 80 Met Leu Leu Gly Ala Ile Glu Ala Gly Gly Thr Lys Phe Val Cys Ala 1 5 10 15 Thr Gly Ala Glu Asn Gly Gln Val Ser Asp Arg Ile Ser Ile Pro Thr 20 25 30 Thr Thr Pro Val Glu Thr Met Thr Ala Val Asp Asp Tyr Phe Thr Thr 35 40 45 His Pro Val Asp Ala Ile Gly Ile Gly Ser Phe Gly Pro Ile Gly Val 50 55 60 Asn Pro His Asp Pro Lys Tyr Gly Tyr Ile Thr Thr Thr Pro Lys Pro 65 70 75 80 Gly Trp Gly Asp Phe Asp Phe Leu Gly His Leu Lys Ser Gln Phe Asn 85 90 95 Ile Pro Leu Tyr Trp Thr Thr Asp Val Asn Glu Ala Ala Tyr Gly Glu 100 105 110 Ser Met Ile Gly Ile Ala Lys Asp Val Pro Asn Ser Ile Tyr Met Thr 115 120 125 Ile Gly Thr Gly Val Gly Ala Gly Val Ile Ser Gln Asn His Ile Phe 130 135 140 Asn Gly Arg Thr His Thr Glu Leu Gly His Met Arg Leu Asn Arg Leu 145 150 155 160 Pro Gly Asp Asp Phe Lys Ser Asn Cys Pro Tyr His Asp Ile Cys Leu 165 170 175 Glu Gly Leu Ala Ala Gly Pro Ala Val Gly Lys Arg Thr Gly Lys Ala 180 185 190 Gly Lys Asp Ile Pro Val Asp Asp Pro Val Trp Pro Ile Ile Thr Asp 195 200 205 Tyr Ile Ala Gln Ala Cys Val Asn Leu Thr Val Ala Phe Ala Pro Asp 210 215 220 Lys Ile Ile Leu Asn Gly Gly Val Met Asn Gln Arg Gln Leu Phe Pro 225 230 235 240 Met Ile Arg Glu Lys Phe Ala Ala Tyr Leu Asn Gly Tyr Glu Glu Val 245 250 255 Pro Pro Leu Asp Asp Tyr Ile Val Pro Ala Gly Leu Gly Asn Asn Ser 260 265 270 Gly Ile Ala Gly Gly Leu Leu Leu Ala Gln Ala Ala Leu Lys Asn Ala 275 280 285 <210> 81 <211> 326 <212> PRT <213> Rhizobium leguminosarum bv. trifolii <400> 81 Met Ile Leu Cys Cys Gly Glu Ala Leu Ile Asp Met Leu Pro Arg Asp 1 5 10 15 Thr Thr Leu Gly Glu Lys Gly Phe Ala Pro Tyr Ala Gly Gly Ala Ile 20 25 30 Phe Asn Thr Ala Ile Ala Leu Gly Arg Leu Gly Ile Pro Thr Ala Phe 35 40 45 Phe Thr Gly Ile Ala Asp Asp Met Met Gly Glu Ile Leu Leu Glu Thr 50 55 60 Leu Lys Ala Ser Asn Val Asp Tyr Ser Pro Cys Ala Ile Thr Pro Arg 65 70 75 80 Pro Ser Thr Ile Ala Phe Val Lys Leu Val Asn Gly Gln Ala Thr Tyr 85 90 95 Ala Phe Tyr Asp Glu Gly Thr Ala Gly Arg Met Ile Thr Thr Ala Asp 100 105 110 Leu Pro Asp Leu Gly Asp Asp Cys Glu Ala Leu His Phe Gly Ala Ile 115 120 125 Ser Leu Ile Pro Ser Pro Cys Gly Glu Thr Tyr Glu Ala Leu Leu Asp 130 135 140 Arg Glu Ala Ala Ser Arg Val Ile Ser Leu Asp Pro Asn Ile Arg Pro 145 150 155 160 Gly Phe Ile Lys Asp Lys Pro Ser His Met Ala Arg Ile Lys Arg Met 165 170 175 Ala Ala Lys Ser Asp Ile Val Lys Phe Ser Asp Glu Asp Leu Asp Trp 180 185 190 Phe Gly Leu Gln Gly Asp His Asp Ala Leu Ala Ala His Trp Leu Asn 195 200 205 His Gly Ala Lys Leu Val Val Ile Thr Lys Gly Ala Glu Gly Ala Ser 210 215 220 Gly Tyr Thr Lys Asp Arg Lys Val Thr Val Pro Ser Glu Arg Val Thr 225 230 235 240 Val Val Asp Thr Val Gly Ala Gly Asp Thr Phe Asp Ala Gly Ile Leu 245 250 255 Ala Ser Leu Lys Met Asp Asn Leu Leu Thr Lys Arg Gln Val Ala Ser 260 265 270 Leu Asp Glu Gln Ala Leu Arg Asn Gly Pro Asp Pro Arg Arg Gln Ser 275 280 285 Arg Arg Arg His Arg Leu Pro Arg Arg Arg Gln Ser Thr Leu Gly Ala 290 295 300 Arg Asp Trp Ser Leu Arg Leu Glu Gln Asp Ser Asp Pro His Pro Pro 305 310 315 320 Asp Asp Thr Phe Ser Pro 325 <210> 82 <211> 221 <212> PRT <213> Salmonella thompson <400> 82 Val Asp Leu Asp Asp Gln Gly Glu Arg Thr Phe Thr Phe Met Val Arg 1 5 10 15 Pro Ser Ala Asp Leu Phe Leu Val Glu Glu Asp Leu Pro Gln Phe Ala 20 25 30 Ala Gly Gln Trp Leu His Val Cys Ser Ile Ala Leu Ser Ala Glu Pro 35 40 45 Ser Arg Ser Thr Thr Phe Ala Ala Met Glu Ser Ile Arg Ser Ala Gly 50 55 60 Gly Arg Val Ser Phe Asp Pro Asn Ile Arg Pro Asp Leu Trp Gln Asp 65 70 75 80 Gln Ala Leu Leu Leu Ala Cys Leu Asp Arg Ala Leu His Met Ala Asn 85 90 95 Val Val Lys Leu Ser Glu Glu Glu Leu Val Phe Ile Ser Ser Ser Asn 100 105 110 Asp Leu Ala Tyr Gly Ile Ala Ser Val Thr Glu Arg Tyr Gln Pro Glu 115 120 125 Leu Leu Leu Val Thr Arg Gly Lys Ala Gly Val Leu Ala Ala Phe Gln 130 135 140 Gln Lys Phe Thr His Phe Asn Ala Arg Pro Val Ala Ser Val Asp Thr 145 150 155 160 Thr Gly Ala Gly Asp Ala Phe Val Ala Gly Leu Leu Ala Ser Leu Ala 165 170 175 Ala Asn Gly Met Pro Thr Asp Met Thr Ala Leu Glu Pro Thr Leu Thr 180 185 190 Leu Ala Gln Thr Cys Gly Ala Leu Ala Thr Thr Ala Lys Gly Ala Met 195 200 205 Thr Ala Leu Pro Tyr Gln Arg Asp Leu Asn Arg Gln Phe 210 215 220 <210> 83 <211> 307 <212> PRT <213> Salmonella typhimurium <400> 83 Met Asn Ala Lys Val Trp Val Leu Gly Asp Ala Val Val Asp Leu Leu 1 5 10 15 Pro Glu Ser Glu Gly Arg Leu Leu Gln Cys Pro Gly Gly Ala Pro Ala 20 25 30 Asn Val Ala Val Gly Val Ala Arg Leu Gly Gly Asn Ser Gly Phe Ile 35 40 45 Gly Ala Val Gly Gly Asp Pro Phe Gly Arg Tyr Met Arg His Thr Leu 50 55 60 Gln Gln Glu Gln Val Asp Val Ser His Met Tyr Leu Asp Asp Gln His 65 70 75 80 Arg Thr Ser Thr Val Val Val Asp Leu Asp Asp Gln Gly Glu Arg Thr 85 90 95 Phe Thr Phe Met Val Arg Pro Ser Ala Asp Leu Phe Leu Val Glu Glu 100 105 110 Asp Leu Pro Gln Phe Ala Ala Gly Gln Trp Leu His Val Cys Ser Ile 115 120 125 Ala Leu Ser Ala Glu Pro Ser Arg Ser Thr Thr Phe Ala Ala Met Glu 130 135 140 Ser Ile Arg Ser Ala Gly Gly Arg Val Ser Phe Asp Pro Asn Ile Arg 145 150 155 160 Pro Asp Leu Trp Gln Asp Gln Ala Leu Leu Leu Ala Cys Leu Asp Arg 165 170 175 Ala Leu His Met Ala Asn Val Val Lys Leu Ser Glu Glu Glu Leu Val 180 185 190 Phe Ile Ser Ser Ser Asn Asp Leu Ala Tyr Gly Ile Ala Ser Val Thr 195 200 205 Glu Arg Tyr Gln Pro Glu Leu Leu Leu Val Thr Arg Gly Lys Ala Gly 210 215 220 Val Leu Ala Ala Phe Gln Gln Lys Phe Thr His Phe Asn Ala Arg Pro 225 230 235 240 Val Ala Ser Val Asp Thr Thr Gly Ala Gly Asp Ala Phe Val Ala Gly 245 250 255 Leu Leu Ala Ser Leu Ala Ala Asn Gly Met Pro Thr Asp Met Thr Ala 260 265 270 Leu Glu Pro Thr Leu Thr Leu Ala Gln Thr Cys Gly Ala Leu Ala Thr 275 280 285 Thr Ala Lys Gly Ala Met Thr Ala Leu Pro Tyr Gln Arg Asp Leu Asn 290 295 300 Arg Gln Phe 305 <210> 84 <211> 293 <212> PRT <213> Streptococcus mutans serotype c <400> 84 Met Ser Lys Leu Tyr Gly Ser Ile Glu Ala Gly Gly Thr Lys Phe Val 1 5 10 15 Cys Ala Val Gly Asp Glu Asn Phe Gln Ile Leu Glu Lys Val Gln Phe 20 25 30 Pro Thr Thr Thr Pro Tyr Glu Thr Ile Glu Lys Thr Val Ala Phe Phe 35 40 45 Lys Lys Phe Glu Ala Asp Leu Ala Ser Val Ala Ile Gly Ser Phe Gly 50 55 60 Pro Ile Asp Ile Asp Gln Asn Ser Asp Thr Tyr Gly Tyr Ile Thr Ser 65 70 75 80 Thr Pro Lys Pro Asn Trp Ala Asn Val Asp Phe Val Gly Leu Ile Ser 85 90 95 Lys Asp Phe Lys Ile Pro Phe Tyr Phe Thr Thr Asp Val Asn Ser Ser 100 105 110 Ala Tyr Gly Glu Thr Ile Ala Arg Ser Asn Val Lys Ser Leu Val Tyr 115 120 125 Tyr Thr Ile Gly Thr Gly Ile Gly Ala Gly Thr Ile Gln Asn Gly Glu 130 135 140 Phe Ile Gly Gly Met Gly His Thr Glu Ala Gly His Val Tyr Met Ala 145 150 155 160 Pro His Pro Asn Asp Val His His Gly Phe Val Gly Thr Cys Pro Phe 165 170 175 His Lys Gly Cys Leu Glu Gly Leu Ala Ala Gly Pro Ser Leu Glu Ala 180 185 190 Arg Thr Gly Ile Arg Gly Glu Leu Ile Glu Gln Asn Ser Glu Val Trp 195 200 205 Asp Ile Gln Ala Tyr Tyr Ile Ala Gln Ala Ala Ile Gln Ala Thr Val 210 215 220 Leu Tyr Arg Pro Gln Val Ile Val Phe Gly Gly Gly Val Met Ala Gln 225 230 235 240 Glu His Met Leu Asn Arg Val Arg Glu Lys Phe Thr Ser Leu Leu Asn 245 250 255 Asp Tyr Leu Pro Val Pro Asp Val Lys Asp Tyr Ile Val Thr Pro Ala 260 265 270 Val Ala Glu Asn Gly Ser Ala Thr Leu Gly Asn Leu Ala Leu Ala Lys 275 280 285 Lys Ile Ala Ala Arg 290 <210> 85 <211> 307 <212> PRT <213> Vibrio alginolyticus <400> 85 Met Asn Gln Val Trp Val Thr Gly Asp Ala Val Val Asp Leu Ile Pro 1 5 10 15 Glu Ser Glu Thr Ser Leu Leu Lys Cys Pro Gly Gly Ala Pro Ala Asn 20 25 30 Val Ala Val Ala Ile Ala Arg Leu Ser Gly Lys Ser Ala Phe Phe Gly 35 40 45 Arg Val Gly Asp Asp Pro Phe Gly Arg Phe Met Gln Ser Ile Leu Asp 50 55 60 Gln Glu Gly Val Cys Thr Glu Phe Leu Ile Lys Asp Pro Glu Gln Arg 65 70 75 80 Thr Ser Thr Val Val Val Asp Leu Asp Asp Gln Gly Glu Arg Ser Phe 85 90 95 Thr Phe Met Val Lys Pro Ser Ala Asp Gln Phe Met Ser Val Glu Asp 100 105 110 Met Gly Asn Phe Lys Gln Gly Asp Trp Leu His Val Cys Ser Ile Ser 115 120 125 Leu Ala Asn Glu Pro Ser Arg Ser Ser Thr Phe Glu Ala Ile Lys Arg 130 135 140 Ala Lys Ala Ala Gly Gly Phe Ile Ser Phe Asp Pro Asn Leu Arg Asp 145 150 155 160 Glu Val Trp Gln Asp Gln Ser Glu Ile Gln Ala Val Val Met Lys Ala 165 170 175 Val Ala Met Ala Asp Val Val Lys Phe Ser Glu Glu Glu Leu Leu Phe 180 185 190 Leu Thr Asp Ser Thr Ser Met Ala Gln Gly Leu Gln Gln Ile Ala Ala 195 200 205 Met Asn Ile Ala Leu Val Leu Val Thr Gln Gly Ala Lys Gly Val Trp 210 215 220 Arg Val Phe Glu Ser Gln Ser Glu Leu Ile Thr Gly Gln Val Val Ser 225 230 235 240 Pro Ile Asp Thr Thr Gly Ala Gly Asp Ala Phe Val Gly Gly Leu Leu 245 250 255 Ala Cys Leu Ser Arg His Ala Asp Trp Lys Asn His Pro Val Val Ser 260 265 270 Ser Ala Ile Gln Trp Ala Asn Gly Cys Gly Ala Leu Ala Thr Thr Gln 275 280 285 Lys Gly Ala Met Thr Ala Leu Pro Thr Gln Thr Glu Leu Leu Arg Phe 290 295 300 Ile Gly Gln 305 <210> 86 <211> 301 <212> PRT <213> Zymomonas mobilis subsp. mobilis <400> 86 Met Lys Asn Asp Lys Lys Ile Tyr Gly Cys Ile Glu Gly Gly Gly Thr 1 5 10 15 Lys Phe Met Leu Ala Leu Ile Asp Ser Asp Arg Lys Met Leu Ala Val 20 25 30 Glu Arg Val Pro Thr Thr Thr Pro Glu Glu Thr Leu Gly Lys Ser Val 35 40 45 Glu Phe Phe Lys Lys Ala Leu Pro Gln Tyr Ala Asp Ser Phe Ala Ser 50 55 60 Phe Gly Ile Ala Ser Phe Gly Pro Leu Cys Leu Asp Arg Lys Ser Pro 65 70 75 80 Lys Trp Gly Tyr Ile Thr Asn Thr Pro Lys Pro Phe Trp Pro Asn Thr 85 90 95 Asp Val Val Thr Pro Phe Lys Glu Ala Phe Gly Cys Pro Val Glu Ile 100 105 110 Asp Thr Asp Val Asn Gly Ala Ala Leu Ala Glu Asn Phe Trp Gly Ala 115 120 125 Ser Lys Gly Thr His Thr Ser Val Tyr Val Thr Val Gly Thr Gly Phe 130 135 140 Gly Gly Gly Val Leu Ile Asp Gly Lys Pro Ile His Gly Leu Ala His 145 150 155 160 Pro Glu Met Gly His Gly Ile Pro Ile Arg His Pro Asp Asp Arg Asp 165 170 175 Phe Glu Gly Cys Cys Pro Tyr His Gly Gly Cys Tyr Glu Gly Leu Ala 180 185 190 Ser Gly Thr Ala Ile Arg Lys Arg Trp Gly Lys Ala Leu Asn Glu Met 195 200 205 Glu Pro Ala Glu Phe Glu Lys Ala Arg Glu Ile Ile Ala Phe Tyr Leu 210 215 220 Ala His Phe Asn Val Thr Leu Gln Ala Phe Ile Ser Pro Glu Arg Ile 225 230 235 240 Val Phe Gly Gly Gly Val Met His Val Asp Gly Met Leu Ala Ser Val 245 250 255 Arg Arg Gln Thr Ala Glu Ile Ala Asn Ser Tyr Phe Glu Gly Ala Asp 260 265 270 Phe Glu Lys Ile Ile Val Leu Pro Gly Leu Gly Asp Gln Ala Gly Met 275 280 285 Met Gly Ala Phe Ala Leu Ala Leu Ala Ala Glu Asn Lys 290 295 300 <210> 87 <211> 484 <212> PRT <213> Candida albicans <400> 87 Met Val His Leu Gly Pro Lys Pro Ala Gln Lys Arg Lys Gly Thr Phe 1 5 10 15 Thr Asp Val Ser Pro Gln Leu Leu Glu Ala Leu Lys Pro Ile Gln Glu 20 25 30 Gln Phe Thr Ile Ser Ala Asp Lys Leu Arg Ala Ile Val Lys His Phe 35 40 45 Ile Ser Glu Leu Asp Arg Gly Leu Ser Lys Ala Gly Gly Asn Ile Pro 50 55 60 Met Ile Pro Gly Trp Val Met Asp Phe Pro Thr Gly Lys Glu Thr Gly 65 70 75 80 Ser Tyr Leu Ala Ile Asp Leu Gly Gly Thr Asn Leu Arg Val Val Leu 85 90 95 Val Lys Leu Gly Gly Asn Arg Asp Phe Asp Thr Thr Gln Ser Lys Phe 100 105 110 Ala Leu Pro Ala His Met Arg Thr Ala Thr Ser Asp Glu Leu Trp Asp 115 120 125 Phe Ile Ala Lys Cys Leu Lys Glu Phe Val Asp Glu Ile Tyr Pro Asp 130 135 140 Gly Cys Ser Glu Pro Leu Pro Leu Gly Phe Thr Phe Ser Tyr Pro Ala 145 150 155 160 Ser Gln Asn Arg Ile Asn Glu Gly Ile Leu Gln Arg Trp Thr Lys Gly 165 170 175 Trp Ser Ile Asp Gly Ile Glu Gly Lys Asp Val Val Pro Met Leu Gln 180 185 190 Lys Ala Ile Lys Lys Val Gly Val Pro Ile Asp Val Val Ala Leu Ile 195 200 205 Asn Asp Thr Thr Gly Thr Leu Val Ala Ser Met Tyr Thr Asp Pro Glu 210 215 220 Ala Lys Met Gly Leu Ile Phe Gly Thr Gly Val Asn Gly Ala Tyr Phe 225 230 235 240 Asp Val Val Lys Asp Ile Pro Lys Leu Glu Gly Lys Cys Pro Ser Asp 245 250 255 Ile Pro Pro Glu Ser Pro Met Ala Ile Asn Cys Glu Tyr Gly Ser Phe 260 265 270 Asp Asn Glu Lys Tyr Ile Leu Pro Arg Thr Lys Tyr Asp Val Gln Ile 275 280 285 Asp Glu Glu Ser Pro Arg Pro Gly Gln Gln Thr Phe Glu Lys Met Ile 290 295 300 Ser Gly Tyr Tyr Leu Gly Glu Val Leu Arg Leu Ile Leu Leu Glu Phe 305 310 315 320 Ala Glu Glu Lys Lys Leu Ile Phe Lys Gly Gln Asn Leu Asp Lys Leu 325 330 335 Lys Val Pro Tyr Val Met Asp Ala Ser Tyr Pro Ser Lys Ile Glu Glu 340 345 350 Asp Pro Phe Glu Asn Leu Ser Asp Val Ala Asp Leu Phe Arg Glu Lys 355 360 365 Leu Gly Ile Glu Thr Thr Glu Pro Glu Arg Lys Ile Ile Arg Cys Leu 370 375 380 Ala Glu Leu Ile Gly Glu Arg Ser Ala Arg Phe Ser Val Cys Gly Ile 385 390 395 400 Ala Ala Ile Cys Gln Lys Arg Gly Tyr Lys Thr Ala His Cys Ala Ala 405 410 415 Asp Gly Ser Val Tyr Asn Lys Tyr Pro Gly Phe Lys Glu Arg Thr Ala 420 425 430 Gln Ala Leu Arg Asp Ile Tyr Glu Trp Pro Ala Asp Val Lys Asp Pro 435 440 445 Ile Ile Ile Val Pro Ala Glu Asp Gly Ser Gly Val Gly Ala Ala Val 450 455 460 Ile Ala Ala Leu Thr Glu Lys Arg Leu Lys Glu Gly Lys Ser Val Gly 465 470 475 480 Leu Leu Gly Ala <210> 88 <211> 490 <212> PRT <213> Emericella nidulans <400> 88 Met Val Gly Val Gly Pro Lys Arg Pro Pro Ser Arg Lys Gly Ser Met 1 5 10 15 Ser Asp Val Pro Gln Asn Leu Leu Glu His Ile Lys His Phe Glu Glu 20 25 30 Ile Phe Thr Val Asp Thr Ala Thr Leu Lys Lys Ile Val Asp His Phe 35 40 45 Val Asn Glu Leu Thr Lys Gly Leu Ser Val Glu Gly Gly Asn Ile Pro 50 55 60 Met Asn Val Thr Trp Val Leu Gly Phe Pro Asp Gly Lys Glu Thr Gly 65 70 75 80 Thr Phe Leu Ala Leu Asp Met Gly Gly Thr Asn Leu Arg Val Cys Glu 85 90 95 Ile Thr Leu Thr Glu Glu Lys Gly Gly Phe Asp Ile Ile Gln Ser Lys 100 105 110 Tyr Arg Met Pro Glu Glu Leu Lys Thr Gly Glu Ala Glu Glu Leu Trp 115 120 125 Gln Tyr Ile Val Asp Cys Val Glu Gln Phe Ile Gln Phe His His Glu 130 135 140 Asn Glu Asn Leu Ser Lys Leu Pro Leu Gly Phe Thr Phe Ser Tyr Pro 145 150 155 160 Ala Thr Gln Asp Tyr Ile Asp His Gly Val Leu Gln Arg Trp Thr Lys 165 170 175 Gly Phe Asp Ile Asp Gly Val Glu Gly Lys Asp Val Val Pro Pro Leu 180 185 190 Glu Lys Val Phe Lys Glu Arg Gly Leu Pro Ile Lys Val Ala Ala Leu 195 200 205 Ile Asn Asp Thr Thr Gly Thr Leu Ile Ala Ser Ser Tyr Thr Asp Pro 210 215 220 Ala Met Lys Ile Gly Cys Ile Phe Gly Thr Gly Val Asn Ala Ala Tyr 225 230 235 240 Met Glu Asn Ala Gly Ser Ile Pro Lys Leu Ala His Met Asn Leu Pro 245 250 255 Pro Asp Met Pro Val Ala Ile Asn Cys Glu Tyr Gly Ala Phe Asp Asn 260 265 270 Glu His Ile Val Leu Pro Leu Thr Lys Tyr Asp His Ile Ile Asp Arg 275 280 285 Asp Ser Pro Arg Pro Gly Gln Gln Ala Phe Glu Lys Met Thr Ala Gly 290 295 300 Leu Tyr Leu Gly Glu Ile Phe Arg Leu Ala Leu Val Asp Ile Leu Asp 305 310 315 320 Thr Gln Pro Gly Leu Ile Phe Lys Asp Gln Asp Thr Ser Gln Leu Arg 325 330 335 Ile Pro Tyr Leu Leu Asp Ser Ser Phe Pro Ala Ala Ile Glu Glu Asp 340 345 350 Pro Tyr Glu Asn Leu Ile Glu Thr Ala Glu Leu Val Gln Asn Met Leu 355 360 365 Lys Ile Lys Ala Thr Arg Ser Glu Leu Glu Leu Met Arg Arg Leu Ala 370 375 380 Glu Leu Ile Gly Thr Arg Ala Ala Arg Leu Ser Ala Cys Gly Val Ala 385 390 395 400 Ala Ile Cys Lys Lys Lys Asn Ile Glu Ser Cys His Val Gly Ala Asp 405 410 415 Gly Ser Val Phe Thr Lys Tyr Pro His Phe Lys Ala Arg Gly Ala Gln 420 425 430 Ala Leu Arg Glu Ile Leu Asp Trp Ala Pro Ser Glu Lys Asp Lys Val 435 440 445 Thr Ile His Ala Ala Glu Asp Gly Ser Gly Val Gly Ala Ala Leu Ile 450 455 460 Ala Ala Leu Thr Leu Lys Arg Val Lys Ala Gly Asn Thr Ala Gly Ile 465 470 475 480 Arg Asp Ala Gln Ala Met Leu Ala Met Cys 485 490 <210> 89 <211> 485 <212> PRT <213> Kluyveromyces lactis <400> 89 Met Val Arg Leu Gly Pro Lys Lys Pro Pro Ala Arg Lys Gly Ser Met 1 5 10 15 Ala Asp Val Pro Ala Asn Leu Met Glu Gln Ile His Gly Leu Glu Thr 20 25 30 Leu Phe Thr Val Ser Ser Glu Lys Met Arg Ser Ile Val Lys His Phe 35 40 45 Ile Ser Glu Leu Asp Lys Gly Leu Ser Lys Lys Gly Gly Asn Ile Pro 50 55 60 Met Ile Pro Gly Trp Val Val Glu Tyr Pro Thr Gly Lys Glu Thr Gly 65 70 75 80 Asp Phe Leu Ala Leu Asp Leu Gly Gly Thr Asn Leu Arg Val Val Leu 85 90 95 Val Lys Leu Gly Gly Asn His Asp Phe Asp Thr Thr Gln Asn Lys Tyr 100 105 110 Arg Leu Pro Asp His Leu Arg Thr Gly Thr Ser Glu Gln Leu Trp Ser 115 120 125 Phe Ile Ala Lys Cys Leu Lys Glu Phe Val Asp Glu Trp Tyr Pro Asp 130 135 140 Gly Val Ser Glu Pro Leu Pro Leu Gly Phe Thr Phe Ser Tyr Pro Ala 145 150 155 160 Ser Gln Lys Lys Ile Asn Ser Gly Val Leu Gln Arg Trp Thr Lys Gly 165 170 175 Phe Asp Ile Glu Gly Val Glu Gly His Asp Val Val Pro Met Leu Gln 180 185 190 Glu Gln Ile Glu Lys Leu Asn Ile Pro Ile Asn Val Val Ala Leu Ile 195 200 205 Asn Asp Thr Thr Gly Thr Leu Val Ala Ser Leu Tyr Thr Asp Pro Gln 210 215 220 Thr Lys Met Gly Ile Ile Ile Gly Thr Gly Val Asn Gly Ala Tyr Tyr 225 230 235 240 Asp Val Val Ser Gly Ile Glu Lys Leu Glu Gly Leu Leu Pro Glu Asp 245 250 255 Ile Gly Pro Asp Ser Pro Met Ala Ile Asn Cys Glu Tyr Gly Ser Phe 260 265 270 Asp Asn Glu His Leu Val Leu Pro Arg Thr Lys Tyr Asp Val Ile Ile 275 280 285 Asp Glu Glu Ser Pro Arg Pro Gly Gln Gln Ala Phe Glu Lys Met Thr 290 295 300 Ser Gly Tyr Tyr Leu Gly Glu Ile Met Arg Leu Val Leu Leu Asp Leu 305 310 315 320 Tyr Asp Ser Gly Phe Ile Phe Lys Asp Gln Asp Ile Ser Lys Leu Lys 325 330 335 Glu Ala Tyr Val Met Asp Thr Ser Tyr Pro Ser Lys Ile Glu Asp Asp 340 345 350 Pro Phe Glu Asn Leu Glu Asp Thr Asp Asp Leu Phe Lys Thr Asn Leu 355 360 365 Asn Ile Glu Thr Thr Val Val Glu Arg Lys Leu Ile Arg Lys Leu Ala 370 375 380 Glu Leu Val Gly Thr Arg Ala Ala Arg Leu Thr Val Cys Gly Val Ser 385 390 395 400 Ala Ile Cys Asp Lys Arg Gly Tyr Lys Thr Ala His Ile Ala Ala Asp 405 410 415 Gly Ser Val Phe Asn Arg Tyr Pro Gly Tyr Lys Glu Lys Ala Ala Gln 420 425 430 Ala Leu Lys Asp Ile Tyr Asn Trp Asp Val Glu Lys Met Glu Asp His 435 440 445 Pro Ile Gln Leu Val Ala Ala Glu Asp Gly Ser Gly Val Gly Ala Ala 450 455 460 Ile Ile Ala Cys Leu Thr Gln Lys Arg Leu Ala Ala Gly Lys Ser Val 465 470 475 480 Gly Ile Lys Gly Glu 485 <210> 90 <211> 549 <212> PRT <213> Neosartorya fumigata <400> 90 Met Thr Ala Pro Leu Val Lys Val Leu His Arg Met Gln Glu Leu Phe 1 5 10 15 Ser Asn Ile Val Ala Ala Leu Glu Ala Met Ile Val Phe Pro Ser Leu 20 25 30 Phe Arg Asp Pro Ser Arg Lys Arg Arg Lys Thr Phe His Gly Ala Tyr 35 40 45 Trp Arg Arg Arg Thr Leu Asp Asn Phe Ala Glu Glu Val Asp Arg Leu 50 55 60 Phe Thr Ser Pro Leu Ser Leu Arg Asn Met Ile Ile Met Ser Glu Lys 65 70 75 80 Ile Arg Glu Gln Phe Lys Ser Cys Leu Gln Ser Ser Pro Val Cys Met 85 90 95 Leu Pro Ser Tyr Asn His Ala Leu Pro Ser Gly Thr Glu Lys Gly Thr 100 105 110 Tyr Leu Ala Leu Asp Val Gly Gly Ser Thr Phe Arg Val Ala Leu Ile 115 120 125 Glu Leu Gly Gly Ala Gly Ala Met Lys Ile Leu Gln Glu Ser Ser Ser 130 135 140 Pro Ile Asp Asn Asp Val Lys Leu Leu Glu Gly Thr Leu Phe Phe Asp 145 150 155 160 Trp Met Ala Glu Lys Ile Glu Ser Met Leu Ser Ala Val Gly Ala Asp 165 170 175 Tyr Gly Arg Glu Ala Val Pro Leu Ser Met Gly Leu Ser Trp Ser Phe 180 185 190 Pro Ile Glu Gln Thr Ser Ile Ser Ser Gly Leu Val Ile His Met Gly 195 200 205 Lys Gly Phe Leu Cys Ser Asn Gly Thr Leu Gly Gln Glu Leu Gly Asp 210 215 220 Leu Ile Val Gln Ser Cys Arg Arg Arg Ser Leu Asn Val Arg Val Asp 225 230 235 240 Ala Ile Val Asn Asp Ser Ser Ala Ala Leu Leu Ser Arg Ala Tyr Val 245 250 255 Asp Pro Lys Thr Arg Met Ser Leu Ile Leu Gly Thr Gly Thr Asn Val 260 265 270 Ala Val His Phe Pro Val Arg Glu Ile Gly Leu Thr Lys Phe Gly Thr 275 280 285 Arg Pro Pro Gly Trp Phe Asp Tyr Ala Lys His Val Ile Ile Asn Ser 290 295 300 Glu Leu Ser Met Phe Gly Gly Gly Ile Leu Pro Met Thr Arg Trp Asp 305 310 315 320 Asp Ile Leu Asn Gln Thr His Leu Arg Pro Asp Tyr Gln Pro Leu Glu 325 330 335 Tyr Met Val Thr Gly Arg Tyr Leu Gly Glu Ile Val Arg Leu Ile Ile 340 345 350 Thr Glu Ala Val Glu Thr Ala Asn Leu Phe Arg Gly Glu Leu Pro His 355 360 365 Ser Met Arg Asp Pro Tyr Ser Phe Asp Thr Ser Ile Val Ala Phe Leu 370 375 380 Glu Ala Asp Thr Ser Pro Ser Leu Val Pro Ser Ala Ala Leu Leu Gln 385 390 395 400 Lys Glu His Thr Phe Pro Val Ser Pro Ser Val Glu Asp Leu Arg Phe 405 410 415 Leu Arg Arg Ile Cys Gln Ile Val Ser Lys Arg Ala Ala Gly Tyr Leu 420 425 430 Ala Thr Ala Ile His Ser Met Trp Cys Leu Arg Asn Asp Ala Glu Phe 435 440 445 Ser Asp Pro Thr Glu Ser Lys Ala Ser Ser Val Lys Asp Thr Gln Glu 450 455 460 Ile Thr Ile Val Glu Ser Glu Glu Asp Ser Arg Ser Leu Ser Ile Ala 465 470 475 480 Cys Asp Gly Ser Val Ile Asn Lys Tyr Pro Gly Phe Arg Asp Arg Cys 485 490 495 Gln Gly Tyr Leu Asn Gln Leu Thr Gln Gln Thr Asn Ser Ser Gln Gly 500 505 510 Pro Leu Asp Pro Ala Thr Ser Pro Tyr Ile Arg Leu Glu Leu Ala Pro 515 520 525 Glu Ser Ala Ile Leu Gly Ala Ala Val Ala Val Ala Val Ala Val Glu 530 535 540 Asp Gln Lys Ser Gly 545 <210> 91 <211> 485 <212> PRT <213> Saccharomyces cerevisiae <400> 91 Met Val His Leu Gly Pro Lys Lys Pro Gln Ala Arg Lys Gly Ser Met 1 5 10 15 Ala Asp Val Pro Lys Glu Leu Met Asp Glu Ile His Gln Leu Glu Asp 20 25 30 Met Phe Thr Val Asp Ser Glu Thr Leu Arg Lys Val Val Lys His Phe 35 40 45 Ile Asp Glu Leu Asn Lys Gly Leu Thr Lys Lys Gly Gly Asn Ile Pro 50 55 60 Met Ile Pro Gly Trp Val Met Glu Phe Pro Thr Gly Lys Glu Ser Gly 65 70 75 80 Asn Tyr Leu Ala Ile Asp Leu Gly Gly Thr Asn Leu Arg Val Val Leu 85 90 95 Val Lys Leu Ser Gly Asn His Thr Phe Asp Thr Thr Gln Ser Lys Tyr 100 105 110 Lys Leu Pro His Asp Met Arg Thr Thr Lys His Gln Glu Glu Leu Trp 115 120 125 Ser Phe Ile Ala Asp Ser Leu Lys Asp Phe Met Val Glu Gln Glu Leu 130 135 140 Leu Asn Thr Lys Asp Thr Leu Pro Leu Gly Phe Thr Phe Ser Tyr Pro 145 150 155 160 Ala Ser Gln Asn Lys Ile Asn Glu Gly Ile Leu Gln Arg Trp Thr Lys 165 170 175 Gly Phe Asp Ile Pro Asn Val Glu Gly His Asp Val Val Pro Leu Leu 180 185 190 Gln Asn Glu Ile Ser Lys Arg Glu Leu Pro Ile Glu Ile Val Ala Leu 195 200 205 Ile Asn Asp Thr Val Gly Thr Leu Ile Ala Ser Tyr Tyr Thr Asp Pro 210 215 220 Glu Thr Lys Met Gly Val Ile Phe Gly Thr Gly Val Asn Gly Ala Phe 225 230 235 240 Tyr Asp Val Val Ser Asp Ile Glu Lys Leu Glu Gly Lys Leu Ala Asp 245 250 255 Asp Ile Pro Ser Asn Ser Pro Met Ala Ile Asn Cys Glu Tyr Gly Ser 260 265 270 Phe Asp Asn Glu His Leu Val Leu Pro Arg Thr Lys Tyr Asp Val Ala 275 280 285 Val Asp Glu Gln Ser Pro Arg Pro Gly Gln Gln Ala Phe Glu Lys Met 290 295 300 Thr Ser Gly Tyr Tyr Leu Gly Glu Leu Leu Arg Leu Val Leu Leu Glu 305 310 315 320 Leu Asn Glu Lys Gly Leu Met Leu Lys Asp Gln Asp Leu Ser Lys Leu 325 330 335 Lys Gln Pro Tyr Ile Met Asp Thr Ser Tyr Pro Ala Arg Ile Glu Asp 340 345 350 Asp Pro Phe Glu Asn Leu Glu Asp Thr Asp Asp Ile Phe Gln Lys Asp 355 360 365 Phe Gly Val Lys Thr Thr Leu Pro Glu Arg Lys Leu Ile Arg Arg Leu 370 375 380 Cys Glu Leu Ile Gly Thr Arg Ala Ala Arg Leu Ala Val Cys Gly Ile 385 390 395 400 Ala Ala Ile Cys Gln Lys Arg Gly Tyr Lys Thr Gly His Ile Ala Ala 405 410 415 Asp Gly Ser Val Tyr Asn Lys Tyr Pro Gly Phe Lys Glu Ala Ala Ala 420 425 430 Lys Gly Leu Arg Asp Ile Tyr Gly Trp Thr Gly Asp Ala Ser Lys Asp 435 440 445 Pro Ile Thr Ile Val Pro Ala Glu Asp Gly Ser Gly Ala Gly Ala Ala 450 455 460 Val Ile Ala Ala Leu Ser Glu Lys Arg Ile Ala Glu Gly Lys Ser Leu 465 470 475 480 Gly Ile Ile Gly Ala 485 <210> 92 <211> 484 <212> PRT <213> Schizosaccharomyces pombe <400> 92 Met Ser Leu His Asp Ala Tyr His Trp Pro Ser Arg Thr Pro Ser Arg 1 5 10 15 Lys Gly Ser Asn Ile Lys Leu Asn Lys Thr Leu Gln Asp His Leu Asp 20 25 30 Glu Leu Glu Glu Gln Phe Thr Ile Pro Thr Glu Leu Leu His Arg Val 35 40 45 Thr Asp Arg Phe Val Ser Glu Leu Tyr Lys Gly Leu Thr Thr Asn Pro 50 55 60 Gly Asp Val Pro Met Val Pro Thr Trp Ile Ile Gly Thr Pro Asp Gly 65 70 75 80 Asn Glu His Gly Ser Tyr Leu Ala Leu Asp Leu Gly Gly Thr Asn Leu 85 90 95 Arg Val Cys Ala Val Glu Val Gln Gly Asn Gly Lys Phe Asp Ile Thr 100 105 110 Gln Ser Lys Tyr Arg Leu Pro Gln Glu Leu Lys Val Gly Thr Arg Glu 115 120 125 Ala Leu Phe Asp Tyr Ile Ala Asp Cys Ile Lys Lys Phe Val Glu Glu 130 135 140 Val His Pro Gly Lys Ser Gln Asn Leu Glu Ile Gly Phe Thr Phe Ser 145 150 155 160 Tyr Pro Cys Val Gln Arg Ser Ile Asn Asp Ala Ser Leu Val Ala Trp 165 170 175 Thr Lys Gly Phe Asp Ile Asp Gly Val Glu Gly Glu Ser Val Gly Pro 180 185 190 Leu Leu Ser Ala Ala Leu Lys Arg Val Gly Cys Asn Asn Val Arg Leu 195 200 205 Asn Ala Ile Leu Ser Asp Thr Thr Gly Thr Leu Val Ala Ser Asn Tyr 210 215 220 Ala Ser Pro Gly Thr Glu Ile Gly Val Ile Phe Gly Thr Gly Cys Asn 225 230 235 240 Ala Cys Tyr Ile Glu Lys Phe Ser Glu Ile Pro Lys Leu His Lys Tyr 245 250 255 Asp Phe Pro Glu Asp Met Asn Met Ile Ile Asn Cys Glu Trp Cys Asp 260 265 270 Phe Asp Asn Gln His Val Val Leu Pro Arg Thr Lys Tyr Asp Val Ala 275 280 285 Ile Asp Glu Glu Ser Pro Arg Pro Gly Leu Gln Thr Tyr Glu Lys Met 290 295 300 Ile Ala Gly Cys Tyr Leu Gly Asp Ile Leu Arg Arg Ile Leu Leu Asp 305 310 315 320 Leu Tyr Glu Gln Gly Ala Leu Phe Asn Gly Gln Asp Val Thr Lys Ile 325 330 335 Arg Asp Pro Leu Ala Met Asp Thr Ser Val Leu Ser Ala Ile Glu Val 340 345 350 Asp Pro Phe Glu Asn Leu Asp Glu Thr Gln Thr Leu Phe Glu Glu Thr 355 360 365 Tyr Gly Leu Lys Thr Thr Glu Glu Glu Arg Gln Phe Ile Arg Arg Ala 370 375 380 Cys Glu Leu Ile Gly Thr Arg Ser Ala Arg Leu Ser Ala Cys Gly Val 385 390 395 400 Cys Ala Leu Val Arg Lys Met Asn Lys Pro Ser Met Ile Val Gly Thr 405 410 415 Asp Gly Ser Val Tyr Asn Leu Tyr Pro Arg Phe Lys Asp Arg Leu Ala 420 425 430 Gln Ala Phe Lys Asp Ile Leu Gly Glu Glu Ile Gly Ser Lys Val Val 435 440 445 Thr Ile Pro Ala Glu Asp Gly Ser Gly Val Gly Ala Ala Leu Val Ser 450 455 460 Ala Leu Glu Ala Lys Gly Lys Ala Leu Thr Ser Asp Ile Leu Ala Glu 465 470 475 480 His Leu Lys Asn <210> 93 <211> 478 <212> PRT <213> Schwanniomyces occidentalis <400> 93 Met Val His Leu Gly Pro Lys Pro Pro Gln His Arg Lys Gly Ser Phe 1 5 10 15 Leu Asp Val Pro Glu Tyr Leu Leu Lys Glu Leu Thr Glu Leu Glu Gly 20 25 30 Leu Leu Thr Val Ser Gly Glu Thr Leu Arg Lys Ile Thr Asp His Phe 35 40 45 Ile Ser Glu Leu Glu Lys Gly Leu Ser Lys Gln Gly Gly Asn Ile Pro 50 55 60 Met Ile Pro Gly Trp Val Met Asp Phe Pro Thr Gly Lys Glu Met Gly 65 70 75 80 Asp Tyr Leu Ala Ile Asp Leu Gly Gly Thr Asn Leu Arg Val Val Leu 85 90 95 Val Lys Leu Gly Gly Asn Arg Asp Phe Asp Thr Thr Gln Ser Lys Phe 100 105 110 Ala Leu Pro Glu Asn Met Arg Thr Ala Lys Ser Glu Glu Leu Trp Glu 115 120 125 Phe Ile Ala Glu Cys Leu Gln Lys Phe Val Glu Glu Glu Phe Arg Asn 130 135 140 Gly Val Leu Ser Asn Leu Pro Leu Gly Phe Thr Phe Ser Tyr Pro Ala 145 150 155 160 Ser Gln Gly Ser Ile Asn Glu Gly Tyr Leu Gln Arg Trp Thr Lys Gly 165 170 175 Phe Asp Ile Glu Gly Val Glu Gly His Asp Val Val Pro Met Leu Gln 180 185 190 Ala Ala Ile Glu Lys Arg Lys Val Pro Ile Glu Val Val Ala Leu Ile 195 200 205 Asn Asp Thr Thr Gly Thr Leu Val Ala Ser Met Tyr Thr Asp Pro Glu 210 215 220 Ala Lys Met Gly Leu Phe Ser Gly Thr Gly Cys Asn Gly Ala Tyr Tyr 225 230 235 240 Asp Val Val Asp Asn Ile Pro Lys Leu Glu Gly Lys Val Pro Asp Asp 245 250 255 Ile Lys Ser Ser Ser Pro Met Ala Ile Asn Cys Glu Tyr Gly Ala Phe 260 265 270 Asp Asn Glu His Ile Ile Leu Pro Arg Thr Lys Tyr Asp Ile Gln Ile 275 280 285 Asp Glu Glu Ser Pro Arg Pro Gly Gln Gln Ala Phe Glu Lys Met Ile 290 295 300 Ser Gly Tyr Tyr Leu Gly Glu Val Leu Arg Leu Ile Leu Leu Asp Leu 305 310 315 320 Thr Ser Lys Gln Leu Ile Phe Lys Asp Gln Asp Leu Ser Lys Leu Gln 325 330 335 Val Pro Phe Ile Leu Asp Thr Ser Ile Pro Ala Arg Ile Glu Glu Asp 340 345 350 Pro Phe Glu Asn Leu Ser Asp Val Gln Glu Leu Phe Gln Glu Ile Leu 355 360 365 Gly Ile Gln Thr Thr Ser Pro Glu Arg Lys Ile Ile Arg Arg Leu Ala 370 375 380 Glu Leu Ile Gly Glu Arg Ser Ala Arg Leu Ser Ile Cys Gly Ile Ala 385 390 395 400 Ala Ile Cys Lys Lys Arg Gly Tyr Lys Thr Ala His Cys Ala Ala Asp 405 410 415 Gly Ser Val Tyr Asn Lys Tyr Pro Gly Phe Lys Glu Arg Ala Ala Lys 420 425 430 Gly Leu Arg Asp Ile Phe Gln Trp Glu Ser Glu Glu Asp Pro Ile Val 435 440 445 Ile Val Pro Ala Glu Asp Gly Leu Gly Ala Gly Ala Ala Ile Ile Ala 450 455 460 Ala Leu Thr Glu Lys Arg Leu Lys Asp Gly Leu Pro Leu Val 465 470 475 <110> CHEBIGEN INC. <120> Composition for production of tagatose from fructose using enzyme          combination reaction with substrates and uses thereof <130> PN13297 <160> 93 <170> Kopatentin 2.0 <210> 1 <211> 354 <212> PRT <213> Alcanivorax borkumensis <400> 1 Met Ala Leu Val Ser Met Arg Gln Leu Leu Asp His Ala Ala Glu Phe   1 5 10 15 Gly Tyr Gly Val Ala Tyr Asn Val Asn Val Glu Gln Met Arg              20 25 30 Ala Ile Met Glu Ala Ala Asp Lys Thr Asn Ser Pro Val Ile Val Gln          35 40 45 Ala Ser Ala Gly Ala Arg Lys Tyr Ala Gly Ala Pro Phe Leu Arg His      50 55 60 Leu Ile Leu Ala Ala Thr Glu Glu Phe Pro His Ile Pro Val Val Met  65 70 75 80 His Gln Asp His Gly Thr Ser Pro Ser Val Cys Gln Arg Ser Ile Gln                  85 90 95 Leu Gly Phe Ser Ser Val Met Met Asp Gly Ser Leu Gly Glu Asp Gly             100 105 110 Lys Thr Pro Thr Asp Tyr Glu Tyr Asn Val Glu Val Thr Arg Arg Thr         115 120 125 Val Asp Met Ala His Ala Cys Gly Val Ser Val Glu Gly Glu Leu Gly     130 135 140 Cys Leu Gly Ser Leu Glu Thr Gly Glu Ala Gly Glu Glu Asp Gly Ile 145 150 155 160 Gly Ala Ala Gly Lys Leu Thr His Glu Gln Met Leu Thr Asp Pro Glu                 165 170 175 Glu Ala Ala Asp Phe Val Gln Lys Thr Lys Val Asp Ala Leu Ala Ile             180 185 190 Ala Ile Gly Thr Ser His Gly Ala Tyr Lys Phe Thr Arg Pro Pro Thr         195 200 205 Gly Asp Ile Leu Ala Ile Glu Gln Ile Lys Ala Ile His Lys Arg Ile     210 215 220 Pro Asp Thr His Leu Val Met His Gly Ser Ser Ser Val Pro Gln Asp 225 230 235 240 Trp Leu Ala Ile Ile Asn Glu Phe Gly Gly Glu Ile Pro Glu Thr Tyr                 245 250 255 Gly Val Pro Val Glu Glu Ile Gln Glu Gly Ile Lys His Gly Val Arg             260 265 270 Lys Val Asn Ile Asp Thr Asp Leu Arg Leu Ala Ser Thr Gly Ala Ile         275 280 285 Arg Arg Phe Leu Ala Gln Asn Pro Ala Glu Phe Asp Pro Arg Lys Tyr     290 295 300 Leu Lys Glu Ser Ile Thr Ala Met Arg Asp Ile Cys Met Ala Arg Tyr 305 310 315 320 Glu Ala Phe Gly Thr Ala Gly Tyr Ala Asp Lys Ile Lys Pro Ile Ser                 325 330 335 Leu Glu Lys Met Phe Gln Arg Tyr Glu Ser Gly Asp Leu Asp Pro Arg             340 345 350 Val Lys         <210> 2 <211> 287 <212> PRT <213> Bacillus alcalophilus <400> 2 Met Pro Leu Val Ser Met Thr Glu Met Leu Asn Lys Ala Lys Ala Glu   1 5 10 15 Gly Tyr Ala Val Gly Gln Phe Asn Leu Asn Asn Leu Glu Phe Thr Gln              20 25 30 Ala Ile Leu Gly Ala Ala Glu Glu Glu Lys Ser Pro Ile Leu Gly          35 40 45 Val Ser Glu Gly Ala Ala Arg Tyr Met Gly Gly Phe Lys Thr Val Val      50 55 60 Ser Met Val Lys Ala Leu Met Asp Glu Tyr Asn Val Thr Val Pro Val  65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Leu Glu Lys Cys Val Gln Ala                  85 90 95 Ile His Ala Gly Phe Thr Ser Val Met Ile Asp Gly Ser His His Pro             100 105 110 Leu Ala Glu Asn Ile Ala Ile Thr Lys Arg Val Val Glu Ile Ala His         115 120 125 Ala Val Gly Val Ser Val Glu Ala Glu Leu Gly Arg Ile Gly Gly Gln     130 135 140 Glu Asp Asp Leu Ile Val Asp Asp Ala Glu Ala Ala Tyr Ala Ile Pro 145 150 155 160 Ala Glu Cys Lys Glu Leu Val Ala Ala Thr Gly Val Asp Cys Phe Ala                 165 170 175 Pro Ala Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Asn Leu             180 185 190 Gly Phe Asp His Met Lys Glu Ile Asp Ser Leu Val Gly Ile Pro Leu         195 200 205 Val Leu His Gly Gly Thr Gly Ile Pro Thr Ala Asp Ile Lys Lys Ala     210 215 220 Ile Glu Phe Gly His Ala Lys Ile Asn Val Asn Thr Glu Ser Gln Ile 225 230 235 240 Ser Ser Ala Lys Ala Val Ser Glu Val Seru Ala Ala Asp Thr Asp Val                 245 250 255 Tyr Asp Pro Arg Lys Tyr Leu Gly Pro Ala Arg Glu Ala Ile Lys Glu             260 265 270 Thr Val Lys Gly Lys Met Lys Glu Phe Gly Ser Ser Asn Arg Ala         275 280 285 <210> 3 <211> 285 <212> PRT <213> Bacillus amyloliquefaciens <400> 3 Met Pro Leu Val Ser Met Thr Glu Met Leu Asn Thr Ala Lys Glu Lys   1 5 10 15 Gly Tyr Ala Val Gly Gln Phe Asn Leu Asn Asn Leu Glu Phe Thr Gln              20 25 30 Ala Ile Leu Gln Ala Ala Gln Glu Glu Lys Ser Pro Ile Leu Gly          35 40 45 Val Ser Glu Gly Ala Gly Arg Tyr Met Gly Gly Phe Lys Thr Val Val      50 55 60 Ala Met Val Lys Ala Leu Met Glu Glu Tyr Lys Val Thr Val Val  65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Ser Cys Ala Lys Ala                  85 90 95 Ile His Ala Gly Phe Thr Ser Val Met Ile Asp Ala Ser His His Pro             100 105 110 Phe Glu Glu Asn Val Ala Thr Thr Ala Lys Val Val Glu Leu Ala His         115 120 125 Phe His Gly Val Ser Val Glu Ala Glu Leu Gly Thr Val Gly Gly Gln     130 135 140 Glu Asp Val Ile Ala Glu Gly Val Ile Tyr Ala Asp Pro Lys Glu 145 150 155 160 Cys Gln Glu Leu Val Glu Arg Thr Gly Ile Asp Cys Leu Ala Pro Ala                 165 170 175 Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Asn Leu Gly Phe             180 185 190 Lys Glu Met Glu Asp Ile Gly Lys Thr Thr Gly Leu Pro Leu Val Leu         195 200 205 His Gly Gly Thr Gly Ile Pro Thr Ala Asp Ile Lys Lys Ser Ile Ser     210 215 220 Leu Gly Thr Ala Lys Ile Asn Val Asn Thr Glu Asn Gln Ile Ser Ser 225 230 235 240 Ala Lys Ala Val Arg Glu Thr Leu Ala Ala Lys Pro Asn Glu Tyr Asp                 245 250 255 Pro Arg Lys Tyr Leu Gly Pro Ala Arg Glu Ala Ile Lys Glu Thr Val             260 265 270 Ile Gly Lys Met Arg Glu Phe Gly Ser Ser Asn Gln Ala         275 280 285 <210> 4 <211> 287 <212> PRT <213> Bacillus azotoformans <400> 4 Met Pro Leu Val Ser Met Thr Glu Met Leu Asn Lys Ala Lys Asn Glu   1 5 10 15 Gly Tyr Ala Val Gly Gln Phe Asn Ile Asn Asn Leu Glu Tyr Thr Gln              20 25 30 Ala Ile Leu Gln Ala Ala Gln Ala Glu Asn Ser Pro Ile Leu Gly          35 40 45 Val Ser Glu Gly Ala Ala Lys Tyr Met Gly Gly Phe Lys Thr Val Val      50 55 60 Ala Met Val Lys Gly Leu Met Glu Tyr Tyr Glu Met Thr Val Val  65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Lys Cys Val Glu Ala                  85 90 95 Met Tyr Ala Gly Phe Thr Ser Val Met Ile Asp Gly Ser His Tyr Pro             100 105 110 Leu Glu Glu Asn Ile Glu Leu Thr Lys Arg Val Val Asp Val Ala His         115 120 125 Thr Ile Gly Ile Ser Val Glu Ala Glu Leu Gly Arg Ile Gly Gly Gln     130 135 140 Glu Asp Asp Leu Val Val Asp Asp Ala Glu Ala Met Tyr Ala Ile Pro 145 150 155 160 Ala Glu Cys Asp Arg Leu Val Arg Glu Thr Gly Val Asp Cys Phe Ala                 165 170 175 Pro Ala Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Lys Leu             180 185 190 Gly Phe Asp Arg Met Lys Glu Val Met Glu Leu Thr Gly Val Pro Leu         195 200 205 Val Leu His Gly Gly Thr Gly Ile Pro Thr Lys Asp Ile Gln Lys Ala     210 215 220 Ile Ser Leu Gly Thr Ala Lys Ile Asn Val Asn Thr Glu Asn Gln Ile 225 230 235 240 Thr Ser Thr Lys Thr Val Arg Lys Val Leu Glu Glu Lys Pro Glu Leu                 245 250 255 Tyr Asp Thr Arg Lys Phe Met Gly Pro Ala Arg Asp Thr Ile Lys Glu             260 265 270 Thr Val Ile Gly Lys Ile Arg Glu Phe Gly Ser Ser Asn Lys Ala         275 280 285 <210> 5 <211> 287 <212> PRT <213> Bacillus cellulosilyticus <400> 5 Met Pro Leu Val Ser Met Lys Glu Met Leu Glu Asp Ala Lys Ala Gly   1 5 10 15 Gly Tyr Ala Val Gly Gln Phe Asn Leu Asn Asn Leu Glu Phe Thr Gln              20 25 30 Ala Ile Leu Leu Ala Ala Glu Glu Glu          35 40 45 Val Ser Glu Gly Ala Ala Arg Tyr Met Gly Gly Phe Leu Thr Val Val      50 55 60 Ser Ile Val Glu Ala Leu Met Ala Asp Tyr Glu Ile Thr Val Val  65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Lys Cys Val Glu Ala                  85 90 95 Ile His Ala Gly Phe Thr Ser Val Met Ile Asp Gly Ser His His Pro             100 105 110 Leu Glu Glu Asn Ile Ala Leu Thr Lys Gln Val Val Ala Val Ala His         115 120 125 Thr Leu Gly Val Ser Val Glu Ala Glu Leu Gly Arg Ile Gly Gly Gln     130 135 140 Glu Asp Asp Leu Val Val Asp Asp Ala Asp Ala Ala Tyr Ala Ile Pro 145 150 155 160 Ser Glu Cys Asp Gln Leu Val Arg Glu Thr Gly Val Asp Cys Phe Ala                 165 170 175 Pro Ala Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Asn Leu             180 185 190 Gly Phe Asp Arg Met Glu Glu Val Ala Asn Leu Thr Gly Val Pro Leu         195 200 205 Val Leu His Gly Gly Thr Gly Ile Pro Thr His Asp Ile Gln Lys Ala     210 215 220 Ile Ser Leu Gly Thr Ala Lys Ile Asn Val Asn Thr Glu Asn Gln Ile 225 230 235 240 Ser Ser Ala Lys Arg Val Val Glu Val Leu Ala Glu Lys Pro Asn Glu                 245 250 255 Tyr Asp Pro Arg Lys Tyr Leu Gly Pro Ala Arg Asp Ala Ile Lys Glu             260 265 270 Thr Val Ile Gly Lys Met Arg Glu Phe Gly Ser Ser Asn Lys Ala         275 280 285 <210> 6 <211> 285 <212> PRT <213> Bacillus coagulans <400> 6 Met Pro Leu Val Ser Met Thr Glu Met Leu Asn Lys Ala Lys Glu Gly   1 5 10 15 Lys Tyr Ala Val Gly Gln Phe Asn Val Asn Asn Leu Glu Tyr Ala Gln              20 25 30 Ala Ile Leu Met Ala Ala Glu Glu Glu Lys Ser Pro Ile Leu Gly          35 40 45 Val Ser Glu Gly Ala Gly Arg Tyr Met Ser Gly Phe Lys Thr Val Val      50 55 60 Lys Met Val Glu Gly Leu Met Glu Asp Leu Asn Val Thr Val Pro Val  65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Asn Ala Lys Lys Ala                  85 90 95 Ile Asp Ala Gly Phe Thr Ser Val Met Ile Asp Ala Ser His His Pro             100 105 110 Phe Asp Glu Asn Val Glu Ile Thr Ser Lys Val Val Glu Tyr Ala His         115 120 125 Ala His Gly Val Ser Val Glu Ala Glu Leu Gly Thr Val Gly Gly Gln     130 135 140 Glu Asp Val Val Gly Lys Gly Ile Ile Tyr Ala Asp Pro Glu Glu 145 150 155 160 Cys Lys Lys Leu Val Asp Ala Thr Lys Ile Asp Ala Leu Ala Pro Ala                 165 170 175 Leu Gly Ser Val His Gly Pro Tyr His Gly Glu Pro Asn Leu Gly Phe             180 185 190 Lys Glu Met Glu Glu Ile Ser Lys Ile Thr Gly Met Pro Leu Val Leu         195 200 205 His Gly Gly Thr Gly Ile Pro Glu Ala Asp Ile Lys Lys Ala Ile Ser     210 215 220 Leu Gly Thr Ala Lys Ile Asn Val Asn Thr Glu Asn Gln Ile Ala Ser 225 230 235 240 Ala Lys Thr Val Arg Glu Val Leu Ala Ala Asp Pro Asp Val Tyr Asp                 245 250 255 Pro Arg Lys Tyr Leu Gly Pro Ala Arg Glu Thr Ile Lys Gln Thr Val             260 265 270 Ile Gly Lys Ile Arg Glu Phe Gly Ser Asn Gln Lys Ala         275 280 285 <210> 7 <211> 285 <212> PRT <213> Bacillus cytotoxis <400> 7 Met Pro Leu Val Ser Met Lys Glu Met Leu Asn Lys Ala Leu Glu Gly   1 5 10 15 Lys Tyr Ala Val Gly Gln Phe Asn Ile Asn Asn Leu Glu Trp Thr Gln              20 25 30 Ala Ile Leu Ala Ala Ala Glu          35 40 45 Val Ser Glu Gly Ala Ala Arg His Met Thr Gly Phe Lys Thr Val Val      50 55 60 Ala Met Val Lys Ala Leu Ile Glu  65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Lys Cys Lys Glu Ala                  85 90 95 Ile Asp Ala Gly Phe Thr Ser Val Met Ile Asp Ala Ser His His Pro             100 105 110 Phe Glu Glu Asn Val Glu Thr Thr Lys Lys Val Val Glu Tyr Ala His         115 120 125 Ala Arg Asn Val Ser Val Glu Ala Glu Leu Gly Thr Val Gly Gly Gln     130 135 140 Glu Asp Asp Val Ile Ala Asp Gly Ile Ile Tyr Ala Asp Pro Glu Glu 145 150 155 160 Cys Lys His Leu Val Glu Ala Thr Gly Ile Asp Cys Leu Ala Pro Ala                 165 170 175 Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Asn Leu Gly Phe             180 185 190 Lys Glu Met Glu Gln Val Arg Asp Leu Thr Gly Val Pro Leu Val Leu         195 200 205 His Gly Gly Thr Gly Ile Pro Thr Ala Asp Ile Lys Lys Ala Ile Ser     210 215 220 Leu Gly Thr Ser Lys Ile Asn Val Asn Thr Glu Asn Gln Ile Ala Phe 225 230 235 240 Thr Lys Ala Val Arg Glu Val Leu Asn Lys Asp Gln Glu Val Tyr Asp                 245 250 255 Pro Arg Lys Phe Ile Gly Pro Gly Arg Glu Ala Ile Lys Ala Thr Val             260 265 270 Ile Gly Lys Ile Arg Glu Phe Gly Ser Asn Gly Lys Ala         275 280 285 <210> 8 <211> 287 <212> PRT <213> Bacillus halodurans <400> 8 Met Pro Leu Val Ser Met Thr Glu Met Leu Asn Lys Ala Lys Ala Glu   1 5 10 15 Gly Tyr Ala Val Gly Gln Phe Asn Leu Asn Asn Leu Glu Phe Thr Gln              20 25 30 Ala Ile Leu Gln Ala Ala Glu Glu Glu Lys Ser Pro Val Ile Cys Gly          35 40 45 Val Ser Glu Gly Ala Ala Arg Tyr Met Gly Gly Phe Lys Thr Val Val      50 55 60 Gly Met Val Lys Asn Leu Met Gln Glu Tyr Asn Val Thr Val Pro Val  65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Lys Cys Val Glu Ala                  85 90 95 Ile His Ala Gly Phe Thr Ser Val Met Ile Asp Gly Ser His His Pro             100 105 110 Leu Glu Glu Asn Ile Ala Leu Thr Lys Arg Val Val Asp Val Ala His         115 120 125 Ala Leu Gly Val Ser Val Glu Ala Glu Leu Gly Arg Ile Gly Gly Gln     130 135 140 Glu Asp Asp Leu Val Val Asp Glu Ala Glu Ala Ala Tyr Ala Ile Pro 145 150 155 160 Ser Glu Cys Lys Glu Leu Val Glu Ala Thr Gly Val Asp Cys Phe Ala                 165 170 175 Pro Ala Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Asn Leu             180 185 190 Gly Phe Asp Arg Met Lys Glu Ile Asn Glu Leu Val Gly Ile Pro Leu         195 200 205 Val Leu His Gly Gly Thr Gly Ile Pro Thr Lys Asp Ile Gln Lys Ala     210 215 220 Ile Ser Leu Gly His Ala Lys Ile Asn Val Asn Thr Glu Ser Gln Ile 225 230 235 240 Ser Ser Ala Lys Ala Val Arg Glu Val Leu Glu Glu Lys Pro Glu Leu                 245 250 255 Tyr Asp Pro Arg Lys Tyr Leu Gly Pro Ala Arg Glu Ala Ile Lys Asp             260 265 270 Thr Val Lys Gly Lys Met Arg Glu Phe Gly Ser Ser Asn Lys Ala         275 280 285 <210> 9 <211> 283 <212> PRT <213> Bacillus licheniformis <400> 9 Met Leu Thr Asn Thr Lys Lys Met Leu Leu Asp Ala Gln Ala Asn Gly   1 5 10 15 Tyr Ala Val Pro Ala Phe Asn Ile His Asn Leu Glu Thr Val Gln Ala              20 25 30 Val Thr Glu Ala Ala Glu Ala Leu          35 40 45 Thr Pro Gly Thr Phe Ser Tyr Ala Gly Arg Asp Tyr Ile Gln Ala Ile      50 55 60 Ala Glu Thr Ala Ala Ala Arg Cys Ser Ile Pro Ala Leu His Leu  65 70 75 80 Asp His His Glu Glu Ile Asp Asp Ile Arg Glu Ser Leu Ser Leu Gly                  85 90 95 Thr Lys Ser Val Met Ile Asp Ala Ser His His Pro Phe Ala Glu Asn             100 105 110 Thr Ala Ile Thr Ala Ser Val Val Gln Tyr Ala Arg Gln Lys Asp Ala         115 120 125 Thr Val Glu Ala Glu Leu Gly Arg Leu Gly Gly Gln Glu Asp Asp Leu     130 135 140 Ile Val Asp Glu Ala Asp Ala Tyr Tyr Thr Asp Pro Asp Ala Ala Lys 145 150 155 160 Glu Phe Val Glu Lys Thr Gly Ile Asp Ser Leu Ala Val Ala Ile Gly                 165 170 175 Thr Ala His Gly Leu Tyr Ser Lys Glu Pro Asn Ile Asp Phe Glu Arg             180 185 190 Leu Ala Ile Gln Lys Arg Val Ser Val Pro Leu Val Leu His Gly         195 200 205 Ala Ser Gly Ile Ser Glu Gln Asp Val Arg Lys Cys Ile Lys Leu Gly     210 215 220 Cys Ala Lys Val Asn Ile Ala Thr Glu Leu Lys Ile Pro Phe Ser Asp 225 230 235 240 Lys Leu Arg Arg Tyr Leu His Glu His Ala Asp Ala Ser Asp Pro Arg                 245 250 255 Lys Tyr Met Ala Pro Ala Lys Glu Ala Met Lys Lys Ile Val Gln Glu             260 265 270 Lys Ile Leu Met Cys Met Ser Asn Asp Arg Tyr         275 280 <210> 10 <211> 287 <212> PRT <213> Bacillus macauensis <400> 10 Met Pro Leu Val Ser Met Lys Glu Met Leu Glu Lys Gly Lys Ala Glu   1 5 10 15 Gly Tyr Ala Val Gly Gln Phe Asn Ile Asn Asn Leu Glu Phe Thr Gln              20 25 30 Ala Ile Leu Lys Ala Ala Gln Glu Glu Asn Ser Pro Ile Leu Gly          35 40 45 Val Ser Glu Gly Ala Ala Arg Tyr Met Gly Gly Phe Lys Leu Val Val      50 55 60 Ala Met Thr Lys Ala Leu Met Glu Glu Tyr Ser Ile Thr Val  65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Asn Cys Ala Lys Ala                  85 90 95 Ile His Ala Gly Phe Thr Ser Val Met Ile Asp Gly Ser His Phe Pro             100 105 110 Leu Glu Glu Asn Ile Ala Leu Thr Lys Lys Val Val Glu Leu Ala His         115 120 125 Ile His Gly Val Ser Val Glu Ala Glu Leu Gly Arg Ile Gly Gly Gln     130 135 140 Glu Asp Asp Leu Ile Val Asp Asp Ala Asp Ala Ala Tyr Ala Ile Pro 145 150 155 160 Ser Glu Cys Asp Gln Leu Val Arg Glu Thr Gly Val Asp Cys Phe Ala                 165 170 175 Pro Ala Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Asn Leu             180 185 190 Gly Phe Asp Arg Met Lys Glu Val Asn Glu Leu Thr Gly Val Pro Leu         195 200 205 Val Leu His Gly Gly Thr Gly Ile Pro Thr His Asp Ile Gln Lys Ala     210 215 220 Ile Ser Leu Gly Thr Ala Lys Ile Asn Val Asn Thr Glu Ser Gln Ile 225 230 235 240 Ser Ser Phe Asn Ala Ile Asn Glu Phe Ile Ala Ala Asn Pro Asp Lys                 245 250 255 Tyr Asp Pro Arg Lys Tyr Leu Thr Ala Gly Arg Glu Ala Ile Leu Ala             260 265 270 Thr Val Lys Ala Lys Met Arg Glu Phe Gly Ser Ser Asn Lys Ala         275 280 285 <210> 11 <211> 285 <212> PRT <213> Bacillus megaterium <400> 11 Met Pro Leu Val Ser Met Lys Glu Met Leu Ile Thr Ala Lys Glu Gln   1 5 10 15 Gly Tyr Ala Val Gly Gln Phe Asn Leu Asn Asn Leu Glu Phe Thr Gln              20 25 30 Ala Ile Leu Glu Ala Ala Lys Glu Glu Asn Ser Ser Val Ile Leu Gly          35 40 45 Val Ser Glu Gly Ala Ala Arg Tyr Met Gly Gly Phe Lys Thr Val Val      50 55 60 Ala Met Val Glu Ala Leu Ile Glu Asp Tyr Asp Val Gln Val Val  65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Ser Cys Ala Lys Ala                  85 90 95 Ile His Ala Gly Phe Thr Ser Val Met Ile Asp Ala Ser His His Pro             100 105 110 Phe Glu Glu Asn Ile Ala Ile Thr Ser Lys Val Val Glu Leu Ala His         115 120 125 Phe His Gly Val Ser Val Glu Ala Glu Leu Gly Thr Val Gly Gly Gln     130 135 140 Glu Asp Asp Aslan Glu Gly Aslan Asp Asl Glu 145 150 155 160 Cys Glu Glu Leu Val Asn Arg Thr Gly Ile Asp Cys Leu Ala Pro Ala                 165 170 175 Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Asn Leu Gly Phe             180 185 190 Ala Glu Met Lys Glu Ile Gly Asp Arg Thr Gly Leu Pro Leu Val Leu         195 200 205 His Gly Gly Thr Gly Ile Pro Thr Lys Asp Ile Gln Lys Ser Ser Ser     210 215 220 Leu Gly Thr Ala Lys Ile Asn Val Asn Thr Glu Asn Gln Ile Ala Ser 225 230 235 240 Ala Lys Ala Val Arg Glu Val Leu Ala Ala Gln Pro Glu Gln Tyr Asp                 245 250 255 Pro Arg Lys Tyr Leu Gly Pro Ala Arg Glu Ala Ile Lys Glu Thr Val             260 265 270 Gln Gly Lys Met Arg Glu Phe Gly Ser Ser Asn Lys Ala         275 280 285 <210> 12 <211> 284 <212> PRT <213> Bacillus methanolicus <400> 12 Met Pro Leu Val Ser Met Lys Asp Met Leu Asn His Gly Lys Glu Asn   1 5 10 15 Gly Tyr Ala Val Gly Gln Phe Asn Ile Asn Asn Leu Glu Phe Gly Gln              20 25 30 Ala Ile Leu Gln Ala Ala Glu Glu Glu Lys Ser Pro Val Ile Ile Gly          35 40 45 Val Ser Val Gly Ala Asn Tyr Met Gly Gly Phe Lys Leu Ile Val      50 55 60 Asp Met Val Lys Ser Leu Met Asp Ser Tyr Asn Val Thr Val Pro Val  65 70 75 80 Ala Ile His Leu Asp His Gly Pro Ser Leu Glu Lys Cys Val Gln Ala                  85 90 95 Ile His Ala Gly Phe Thr Ser Val Met Ile Asp Gly Ser His Leu Pro             100 105 110 Leu Glu Glu Asn Ile Glu Leu Thr Lys Arg Val Val Glu Ile Ala His         115 120 125 Ser Val Gly Val Ser Val Glu Ala Glu Leu Gly Arg Ile Gly Gly Gln     130 135 140 Glu Asp Val Val Ala Glu Ser Phe Tyr Ala Ile Pro Ser Glu Cys 145 150 155 160 Glu Gln Leu Val Arg Glu Thr Gly Val Asp Cys Phe Ala Pro Ala Leu                 165 170 175 Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Lys Leu Gly Phe Asp             180 185 190 Arg Met Glu Glu Ile Met Lys Leu Thr Gly Val Pro Leu Val Leu His         195 200 205 Gly Gly Thr Gly Ile Pro Thr Lys Asp Ile Gln Lys Ala Ile Ser Leu     210 215 220 Gly Thr Ala Lys Ile Asn Val Asn Thr Glu Ser Gln Ile Ala Ala Thr 225 230 235 240 Lys Ala Val Arg Glu Val Leu Asn Asn Asp Ala Lys Leu Phe Asp Pro                 245 250 255 Arg Lys Phe Leu Ala Pro Ala Arg Glu Ala Ile Lys Glu Thr Ile Lys             260 265 270 Gly Lys Met Arg Glu Phe Gly Ser Ser Gly Lys Ala         275 280 <210> 13 <211> 298 <212> PRT <213> Bacillus nealsonii <400> 13 Met Asn Asn Gln Leu Glu Lys Met Lys Thr Lys Lys Gly Phe Ile Ala   1 5 10 15 Ala Leu Asp Gln Ser Gly Gly Ser Thr Pro Lys Ala Leu Leu His Tyr              20 25 30 Gly Val Ala Glu Asn Thr Tyr Ala Asn Glu Glu Glu Met Phe Asp Leu          35 40 45 Val His Gln Met Arg Thr Arg Ile Ile Thr Ser Pro Ala Phe Asp Ser      50 55 60 Glu Tyr Ile Leu Gly Ala Ile Leu Phe Glu Gln Thr Met Asp Ser Lys  65 70 75 80 Ile Glu Gly Met Tyr Thr Ala Asp Tyr Leu Ala Glu Lys Lys Gly Ile                  85 90 95 Val Pro Phe Leu Lys Ile Asp Lys Gly Leu Ala Glu Leu Thr Asn Gly             100 105 110 Val Gln Leu Met Lys Pro Ile Thr Asp Leu Asp Asp Ile Leu Arg Arg         115 120 125 Ala Lys Glu Arg His Ile Phe Gly Thr Lys Met Arg Ser Val Ile Lys     130 135 140 Glu Ala Asn Pro Glu Ser Ile Lys Ala Val Val Asp Glu Gln Phe Glu 145 150 155 160 Ile Gly Lys Gln Ile Ile Ala Gly Leu Val Pro Ile Ile Glu Pro                 165 170 175 Glu Val Asp Ile Asn Ser Pro Asp Lys Glu Gln Ser Glu Ala Ile Leu             180 185 190 Lys Gln Glu Leu Leu Lys His Leu His Asn Leu Lys Glu Asp Glu Asn         195 200 205 Val Met Leu Lys Leu Ser Ile Pro Thr Val Ala Asn Thr Tyr Lys Glu     210 215 220 Leu Thr Thr His Ser Arg Val Val Val Val Ala Leu Ser Gly Gly 225 230 235 240 Tyr Ser Arg Glu Glu Ala Asn Gln Lys Leu Lys Glu Asn Asp Asn Ile                 245 250 255 Ile Ala Ser Phe Ser Arg Ala Leu Ser Glu Glu Leu Asn Val Asn Gln             260 265 270 Ser Glu Glu Glu Phe Asn Asn Thr Leu Lys Glu Ala Val Thr Ser Ile         275 280 285 Tyr Glu Ala Ser Thr Ala Lys Thr Gly Arg     290 295 <210> 14 <211> 287 <212> PRT <213> Bacillus pseudofirmus <400> 14 Met Pro Leu Val Ser Met Lys Asp Met Leu Asn Lys Ala Lys Ala Glu   1 5 10 15 Gly Tyr Ala Val Gly Gln Phe Asn Leu Asn Asn Leu Glu Phe Thr Gln              20 25 30 Ala Ile Leu Gln Ala Ala Gln Glu Glu Asn Ser Pro Val Ile Cys Gly          35 40 45 Val Ser Glu Gly Ala Ala Arg Tyr Met Gly Gly Phe Lys Thr Val Val      50 55 60 Lys Met Val Glu Ser Leu Met Glu Glu Tyr Asn Val Thr Val Pro Val  65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Lys Cys Val Glu Ala                  85 90 95 Ile His Ala Gly Phe Thr Ser Val Met Ile Asp Gly Ser His Tyr Pro             100 105 110 Leu Glu Glu Asn Ile Ala Leu Thr Lys Arg Val Val Asp Val Ala His         115 120 125 Ala Leu Gly Val Ser Val Glu Ala Glu Leu Gly Arg Ile Gly Gly Gln     130 135 140 Glu Asp Asp Leu Ile Val Asp Asp Ala Glu Ala Ala Tyr Ala Ile Pro 145 150 155 160 Ser Glu Cys Lys Glu Leu Val Glu Ala Thr Gly Val Asp Cys Phe Ala                 165 170 175 Pro Ala Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Asn Leu             180 185 190 Gly Phe Asp His Met Lys Glu Ile Asp Gly Leu Val Gly Ile Pro Leu         195 200 205 Val Leu His Gly Gly Thr Gly Ile Pro Thr Lys Asp Val Gln Lys Ala     210 215 220 Ile Glu Phe Gly His Ala Lys Ile Asn Val Asn Thr Glu Ser Gln Ile 225 230 235 240 Ala Ser Ala Lys Ala Val Arg Glu Thr Leu Ala Ala Lys Pro Asn Glu                 245 250 255 Tyr Asp Pro Arg Lys Phe Leu Gly Pro Ala Arg Glu Ala Ile Lys Glu             260 265 270 Thr Val Gln Gly Lys Met Arg Glu Phe Gly Ser Ser Asn Lys Ala         275 280 285 <210> 15 <211> 284 <212> PRT <213> Bacillus selenitireducens <400> 15 Met Pro Leu Val Ser Met Thr Asp Met Leu Asn Lys Ala Phe Gln Asn   1 5 10 15 Gln Tyr Ala Val Gly Gln Phe Asn Ile Asn Asn Leu Glu Trp Thr Gln              20 25 30 Ala Ile Leu Gln Ala Ala Glu Glu Glu Gln Ser Pro Ile Leu Gly          35 40 45 Val Ser Glu Gly Ala Ala Lys Tyr Met Gly Gly Phe Lys Leu Ile Thr      50 55 60 Ala Met Val Lys Glu Leu Met Glu Ser Gln Gly Thr Thr Val Val  65 70 75 80 Ala Ile His Leu Asp His Gly Gln Ser Ile Glu Asn Cys Lys Lys Ala                  85 90 95 Val Asp Ala Gly Phe Thr Ser Val Met Ile Asp Gly Ser His His Pro             100 105 110 Ile Glu Glu Asn Ile Glu Met Thr Lys Ala Val Val Glu Tyr Ala His         115 120 125 Ala Arg Gly Ala Ser Val Glu Ala Glu Val Gly Thr Val Gly Gly Glu     130 135 140 Glu Asp Gly Val Ile Gly Gly Val Asn Tyr Ala Asp Leu Glu Glu Cys 145 150 155 160 Val Arg Ile Thr Lys Glu Gly Lys Ala Asp Ala Leu Ala Ala Ala Leu                 165 170 175 Gly Ser Val His Gly Pro Tyr Gln Gly Glu Pro Lys Leu Gly Phe Lys             180 185 190 Glu Met Glu Glu Ile Ala Asn Ala Thr Lys Thr Pro Leu Val Leu His         195 200 205 Gly Gly Ser Gly Ile Pro Thr His Gln Val Lys Lys Ala Ile Glu Phe     210 215 220 Gly His Ala Lys Ile Asn Val Asn Thr Glu Cys Gln Ile Glu Trp Thr 225 230 235 240 Lys Ala Val Arg Glu Lys Leu Ala Asn Asp Glu Lys Val Tyr Asp Pro                 245 250 255 Arg Lys Val Ile Gly Pro Gly Lys Glu Ala Ile Val Arg Val Val Lys             260 265 270 Asp Lys Met Gln Glu Phe Gly Ser Ser Asn Gln Ala         275 280 <210> 16 <211> 290 <212> PRT <213> Bacillus sp. <400> 16 Met Ala Phe Val Ser Met Lys Glu Leu Leu Glu Asp Ala Lys Arg Glu   1 5 10 15 His Tyr Ala Ile Gly Gln Phe Asn Ile Asn Gly Leu Gln Trp Thr Lys              20 25 30 Ala Ile Leu Gln Ala Ala Gln Lys Glu Gln Ser Pro Val Ile Ala Ala          35 40 45 Ala Ser Asp Arg Leu Val His Tyr Leu Gly Gly Phe Lys Thr Ile Ala      50 55 60 Ala Met Val Gly Ala Leu Met Glu  65 70 75 80 Val Leu His Leu Asp His Gly Ser Ser Ala Glu Arg Cys Arg Gln Ala                  85 90 95 Ile Asp Ala Gly Phe Ser Ser Val Met Ile Asp Gly Ser His Gln Pro             100 105 110 Ile Asp Glu Asn Ile Ala Met Thr Lys Glu Val Thr Asp Tyr Ala Ala         115 120 125 Lys His Gly Val Ser Val Glu Ala Glu Val Gly Thr Val Gly Gly Met     130 135 140 Glu Asp Gly Leu Val Gly Gly Val Arg Tyr Ala Asp Ile Lys Glu Cys 145 150 155 160 Glu Arg Ile Val Lys Glu Thr Asn Ile Asp Ala Leu Ala Ala Ala Leu                 165 170 175 Gly Ser Val His Gly Lys Tyr Gln Gly Glu Pro Asn Leu Gly Phe Lys             180 185 190 Glu Met Glu Ala Ile Ser Arg Met Thr His Ile Pro Leu Val Leu His         195 200 205 Gly Ala Ser Gly Ile Pro Gln Asp Gln Ile Lys Lys Ala Ile Thr Leu     210 215 220 Gly His Ala Lys Ile Asn Ile Asn Thr Glu Cys Met Val Ala Trp Thr 225 230 235 240 Asp Glu Thr Arg Arg Met Phe Gln Glu Asn Ser Asp Leu Tyr Glu Pro                 245 250 255 Arg Ala Tyr Met Thr Pro Gly Ile Glu Ala Val Glu Glu Thr Val Arg             260 265 270 Ser Lys Met Arg Glu Phe Gly Ser Ala Gly Lys Ala Ala Lys Gln Gln         275 280 285 Val Gly     290 <210> 17 <211> 290 <212> PRT <213> Bacillus subtilis subsp. inaquosorum <400> 17 Met Ala Phe Val Ser Met Lys Glu Leu Leu Glu Asp Ala Lys Arg Glu   1 5 10 15 Gln Tyr Ala Val Gly Gln Phe Asn Ile Asn Gly Leu Gln Trp Thr Lys              20 25 30 Ala Ile Leu Gln Ala Ala Gln Lys Glu Gln Ser Pro Val Ile Ala Ala          35 40 45 Ala Ser Asp Arg Leu Val Asp Tyr Leu Gly Gly Phe Lys Thr Ile Ala      50 55 60 Ala Met Val Gly Ala Leu Met Glu  65 70 75 80 Val Leu His Leu Asp His Gly Ser Ser Ala Glu Arg Cys Arg Gln Ala                  85 90 95 Ile Asp Ala Gly Phe Ser Ser Val Met Ile Asp Gly Ser His Gln Pro             100 105 110 Ile Asp Glu Asn Ile Val Met Thr Lys Glu Val Thr Asp Tyr Ala Gly         115 120 125 Lys His Gly Val Ser Val Glu Ala Glu Val Gly Thr Val Gly Gly Met     130 135 140 Glu Asp Gly Leu Val Gly Gly Val Arg Tyr Ala Asp Ile Thr Glu Cys 145 150 155 160 Glu Arg Ile Val Lys Glu Thr Asn Ile Asp Ala Leu Ala Ala Ala Leu                 165 170 175 Gly Ser Val His Gly Lys Tyr Gln Gly Glu Pro Asn Leu Gly Phe Lys             180 185 190 Glu Met Glu Ala Ile Ser Arg Met Thr Asp Ile Pro Leu Val Leu His         195 200 205 Gly Ala Ser Gly Ile Pro Gln Asp Gln Ile Lys Lys Ala Ile Thr Leu     210 215 220 Gly His Ala Lys Ile Asn Ile Asn Thr Glu Cys Met Val Ala Trp Thr 225 230 235 240 Asp Glu Thr Arg Arg Met Phe Gln Glu Asn Ser Asp Leu Tyr Glu Pro                 245 250 255 Arg Gly Tyr Met Thr Pro Gly Ile Glu Ala Val Glu Glu Thr Val Arg             260 265 270 Ser Lys Met Arg Glu Phe Gly Ser Ala Gly Lys Ala Ala Lys Gln Gln         275 280 285 Val Gly     290 <210> 18 <211> 285 <212> PRT <213> Bacillus weihenstephanensis <400> 18 Met Pro Leu Val Ser Met Lys Glu Met Leu Asn Thr Ala Leu Glu Gly   1 5 10 15 Lys Tyr Ala Val Gly Gln Phe Asn Met Asn Asn Leu Glu Trp Thr Gln              20 25 30 Ala Ile Leu Ala Ala Ala Glu          35 40 45 Val Ser Glu Gly Ala Ala Arg His Met Thr Gly Phe Lys Thr Val Val      50 55 60 Ala Met Val Lys Ala Leu Ile Glu Glu Met Asn Ile Ser Val Val  65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Lys Cys Lys Glu Ala                  85 90 95 Ile Asp Ala Gly Phe Thr Ser Val Met Ile Asp Ala Ser His His Pro             100 105 110 Phe Asp Glu Asn Val Glu Thr Thr Lys Lys Val Val Glu Tyr Ala His         115 120 125 Ala Arg Asn Val Ser Val Glu Ala Glu Leu Gly Thr Val Gly Gly Gln     130 135 140 Glu Asp Asp Ile Ile Ala Glu Gly Ile Val Ile Tyr Ala Asp Pro Ala Glu 145 150 155 160 Cys Lys His Leu Val Glu Ala Thr Gly Ile Asp Cys Leu Ala Pro Ala                 165 170 175 Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Asn Leu Gly Phe             180 185 190 Ala Glu Met Glu Gln Val Arg Asp Phe Thr Gly Val Pro Leu Val Leu         195 200 205 His Gly Gly Thr Gly Ile Pro Thr Ala Asp Ile Val Lys Ala Ile Ser     210 215 220 Leu Gly Thr Ser Lys Ile Asn Val Asn Thr Glu Asn Gln Ile Glu Phe 225 230 235 240 Thr Lys Ala Val Arg Glu Ala Leu Asn Lys Asp Gln Glu Val Tyr Asp                 245 250 255 Pro Arg Lys Tyr Ile Gly Pro Gly Arg Asp Ala Ile Lys Ala Thr Val             260 265 270 Ala Gly Lys Met Arg Glu Phe Gly Ser Asn Gly Lys Ala         275 280 285 <210> 19 <211> 355 <212> PRT <213> Bifidobacterium breve <400> 19 Met Thr Ile Ala Thr Pro Glu Arg Tyr Ala Glu Met Leu Asp Ala Ala   1 5 10 15 Arg Arg Gly Gly Tyr Ala Tyr Pro Ala Ile Asn Val Ser Ser Ser Gln              20 25 30 Thr Leu Asn Ala Ala Leu Lys Gly Phe Ala Asp Ala Glu Ser Asp Gly          35 40 45 Ile Ile Gln Val Ser Val Gly Ala Ala Ala Tyr Leu Ser Gly Ile Gly      50 55 60 Val Asn Asp Arg Val Thr Gly Ser Leu Ala Leu Ala Ala Phe Ala His  65 70 75 80 Glu Val Ala Lys Tyr Pro Asn Ile Thr Ile Ala Leu His Thr Asp                  85 90 95 His Cys Ala Lys Gln Tyr Leu Asp Glu Trp Val Arg Pro Leu Leu Ala             100 105 110 His Glu Ala Asp Gln Val Ala His Gly Gln Glu Pro Thr Phe Gln Ser         115 120 125 His Met Trp Asp Gly Ser Thr Val Pro Leu Lys Glu Asn Leu Asp Ile     130 135 140 Ala Glu Glu Leu Leu Asp Lys Ser Gln Ala Ala His Thr Val Leu Glu 145 150 155 160 Ile Glu Ile Gly Ala Val Gly Gly Glu Glu Asp Gly Tyr Ser Ala Lys                 165 170 175 Ile Asp Glu Arg Leu Tyr Ser Thr Pro Ala Asp Gly Leu Glu Val Ala             180 185 190 Arg Arg Leu Gly Leu Gly Glu Arg Gly Arg Tyr Met Ala Ala Phe Thr         195 200 205 Phe Gly Asn Val His Gly Ser Tyr Lys Pro Gly Val Val Lys Leu Arg     210 215 220 Pro Ala Leu Leu Gly Asp Ile Gln Ser Arg Val Ala Arg Ala Val Val 225 230 235 240 Glu Gly Glu Leu Pro Ser Ala Ala Gly Ile Val Asp Phe Pro Asn Gly                 245 250 255 Lys Pro Phe Glu Leu Val Phe His Gly Gly Ser Gly Ser Arg Pro Glu             260 265 270 Glu Ile Ala Glu Ala Val Ser His Gly Val Ile Lys Met Asn Ile Asp         275 280 285 Thr Asp Thr Gln Tyr Ser Phe Thr Arg Pro Ile Ala Asp His Val Phe     290 295 300 Glu Asn Tyr Asp Lys Val Leu Lys Ile Asp Gly Glu Val Gly Glu Lys 305 310 315 320 Lys Phe Tyr Asp Pro Arg Ser Trp Gly Arg Lys Ala Glu Glu Ser Met                 325 330 335 Ser Ala Arg Val Ala Glu Ala Cys Lys Gln Leu Gly Ser Ala Gly Lys             340 345 350 Ala Leu Arg         355 <210> 20 <211> 344 <212> PRT <213> Corynebacterium glutamicum <400> 20 Met Pro Ile Ala Thr Pro Glu Val Tyr Asn Glu Met Leu Asp Arg Ala   1 5 10 15 Lys Glu Gly Gly Phe Ala Phe Pro Ala Ile Asn Cys Thr Ser Ser Glu              20 25 30 Thr Ile Asn Ala Ala Leu Lys Gly Phe Ala Glu Ala Glu Ser Asp Gly          35 40 45 Ile Ile Gln Phe Ser Thr Gly Gly Ala Glu Phe Gly Ser Gly Leu Ala      50 55 60 Val Lys Asn Lys Val Lys Gly Ala Val Ala Leu Ala Ala Phe Ala His  65 70 75 80 Glu Ala Ala Lys Ser Tyr Gly Ile Asn Val Ala Leu His Thr Asp His                  85 90 95 Cys Gln Lys Glu Val Leu Asp Glu Tyr Val Arg Pro Leu Leu Ala Ile             100 105 110 Ser Gln Glu Arg Val Asp Arg Gly Glu Leu Pro Leu Phe Gln Ser His         115 120 125 Met Trp Asp Gly Ser Ala Val Pro Ile Asp Glu Asn Leu Glu Ile Ala     130 135 140 Gln Glu Leu Leu Ala Lys Ala Lys Ala Ala Asn Ile Ile Leu Glu Val 145 150 155 160 Glu Ile Gly Val Gly Gly Glu Glu Asp Gly Val Glu Ala Lys Ala                 165 170 175 Gly Ala Asn Leu Tyr Thr Ser Pro Glu Asp Phe Glu Lys Thr Ile Asp             180 185 190 Ala Ile Gly Thr Gly Glu Lys Gly Arg Tyr Leu Leu Ala Ala Thr Phe         195 200 205 Gly Asn Val His Gly Val Tyr Lys Pro Gly Asn Val Lys Leu Arg Pro     210 215 220 Glu Val Leu Leu Glu Gly Gln Gln Val Ala Arg Lys Lys Leu Gly Leu 225 230 235 240 Ala Asp Asp Ala Leu Pro Phe Asp Phe Val Phe His Gly Gly Ser Gly                 245 250 255 Ser Glu Lys Glu Lys Ile Glu Glu Ala Leu Thr Tyr Gly Val Ile Lys             260 265 270 Met Asn Val Asp Thr Asp Thr Gln Tyr Ala Phe Thr Arg Pro Ile Val         275 280 285 Ser His Met Phe Glu Asn Tyr Asn Gly Val Leu Lys Ile Asp Gly Glu     290 295 300 Val Gly Asn Lys Lys Ala Tyr Asp Pro Arg Ser Tyr Met Lys Lys Ala 305 310 315 320 Glu Gln Ser Met Ser Glu Arg Ile Ile Glu Ser Cys Gln Asp Leu Lys                 325 330 335 Ser Val Gly Lys Thr Thr Ser Lys             340 <210> 21 <211> 296 <212> PRT <213> Corynebacterium kroppenstedtii <400> 21 Met Asn Gln Glu Gln Ala Thr Arg Met Thr Glu Ala Lys Gly Phe Ile   1 5 10 15 Ala Ala Leu Asp Gln Ser Gly Gly Ser Thr Pro Lys Ala Leu Arg Ala              20 25 30 Tyr Gly Ile Gln Asp Asp Ala Tyr Ser Ser Asp Ala Glu Met Phe Asp          35 40 45 Leu Val His Ala Met Arg Ser Ser Leu Ile Thr Ser Pro Ser Phe Thr      50 55 60 Ser Glu Lys Val Leu Ala Ile Leu Phe Glu Met Thr Met Asp Arg  65 70 75 80 Glu Ile Glu Gly Ile Pro Thr Ala Gln Tyr Leu Trp Glu Lys Lys Gly                  85 90 95 Ile Val Pro Leu Leu Lys Ile Asp Lys Gly Leu Ala Asp Glu Glu Asn             100 105 110 Asp Val Gln Val Met Lys Pro Met Pro Glu Leu Asp Lys Leu Leu Asp         115 120 125 Arg Gly Val Glu Lys Gly Ile Phe Gly Thr Lys Glu Arg Ser Val Ile     130 135 140 Asn Ala Ala Asn Gly Glu Gly Ile Gln Ala Val Val Asp Gln Gln Phe 145 150 155 160 Glu Ile Gly Lys Gln Val Leu Ala Lys Gly Met Val Pro Ile Leu Glu                 165 170 175 Pro Glu Val Thr Ile Ser Ile Asp Asp Lys Glu Glu Ala Glu Glu Leu             180 185 190 Leu Lys Ala Leu Leu Lys Gly Leu Asp Asp Ile Pro Glu Gly Gln         195 200 205 Gln Val Met Ile Lys Val Ser Ile Pro Thr Val Asp Asn Phe Tyr Glu     210 215 220 Asp Leu Ile Asn His Pro Lys Val Met Val Val Ala Leu Ser Gly 225 230 235 240 Gly Tyr Glu Arg Asp Asp Ala Asn Glu Arg Leu Ser Arg Asn Lys Gly                 245 250 255 Leu Ile Ala Ser Phe Ser Arg Ala Leu Leu Glu Gly Leu Thr Ala Asp             260 265 270 Gln Ser Gln Glu Glu Phe Asp Asn Thr Leu Ala Thr Thr Ile Asp Gly         275 280 285 Ile Tyr Lys Ala Ser Ile Ala Gly     290 295 <210> 22 <211> 289 <212> PRT <213> Enterococcus asini <400> 22 Met Pro Val Val Ser Ser Val Glu Phe Leu Lys Ala Ala Arg Lys Gly   1 5 10 15 Gly Tyr Ala Ile Gly Gly Tyr Asn Thr Asn Asn Leu Glu Trp Thr Gln              20 25 30 Ala Leu Leu Thr Ala Ala Gla Ala Lys Ala Pro Val Leu Ile Gln          35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Val Ile Val      50 55 60 Asp Leu Val Asn Asn Leu Ile Asp Ser Met Gly Ile Thr Val Val  65 70 75 80 Ala Leu His Leu Asp His Gly Asp Tyr Glu Ala Ala Leu Glu Cys Ile                  85 90 95 Glu Val Gly Tyr Ser Ser Val Met Phe Asp Gly Ser His Leu Pro Phe             100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Asp Val Val Glu Lys Ala His Ala         115 120 125 Lys Gly Ile Ala Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu     130 135 140 Asp Gly Ile Gly Aly Gly Glu Leu Ala Asp Ile Asn Glu Cys Val 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Ser Leu Ala Cys Gly Ile Gly Asn                 165 170 175 Ile His Gly Val Tyr Pro Glu Asn Trp Gln Gly Leu Ala Phe Asp His             180 185 190 Leu Gln Glu Ile Ala Ala Ala Val Gly Glu Asp Val Pro Leu Val Leu         195 200 205 His Gly Gly Ser Gly Ile Pro Glu Glu Glu Ile Glu Lys Ala Ile Ser     210 215 220 Met Gly Ile Ser Lys Val Asn Val Asn Thr Glu Phe Gln Leu Ser Phe 225 230 235 240 Ala Lys Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp His Glu Gly                 245 250 255 Lys Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Lys Ala Ala Ile             260 265 270 Ile Lys Asp Ala Glu Glu His Ile Asp Trp Phe Gly Ser Ala Asn Lys         275 280 285 Ala     <210> 23 <211> 288 <212> PRT <213> Enterococcus caccae <400> 23 Met Pro Leu Val Ser Gly Thr Glu Phe Leu Lys Ala Ala Arg Lys Gly   1 5 10 15 Gly Tyr Gly Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Lys              20 25 30 Ala Ile Leu Glu Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln          35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Gln Val Cys Tyr      50 55 60 Asp Leu Val Lys Asp Leu Ile Asp Ser Met Gly Ile Thr Val Val  65 70 75 80 Ala Leu His Leu Asp His Gly Glu Tyr Glu Asp Ala Leu Glu Cys Ile                  85 90 95 Glu Ile Gly Tyr Thr Ser Val Met Phe Asp Gly Ser His Leu Pro Phe             100 105 110 Glu Glu Asn Ile Glu Lys Ala Lys Asp Val Val Ala Lys Ala His Ala         115 120 125 Lys Gly Val Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu     130 135 140 Asp Gly Ile Ile Gly Ser Gly Glu Leu Ala Asp Val Gln Glu Cys Val 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn                 165 170 175 Ile His Gly Ser Tyr Pro Asp Asn Trp Thr Gly Leu Ala Phe Asp His             180 185 190 Leu Gln Glu Ile Ala Asp Ala Val Gly Asp Ile Pro Leu Val Leu His         195 200 205 Gly Gly Ser Gly Ile Pro Leu Glu Gln Val Gln Lys Ala Ile Ser Met     210 215 220 Gly Val Ser Lys Ile Asn Val Asn Thr Glu Cys Gln Glu Val Phe Ala 225 230 235 240 Ala Ala Thr Arg Lys Tyr Ile Glu Glu Gly Lys Asp Leu Glu Gly Lys                 245 250 255 Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Thr Lys Ala Val Thr             260 265 270 Glu Leu Val Glu Gln Arg Ile Glu Trp Phe Gly Ser Ala Asn Lys Ala         275 280 285 <210> 24 <211> 304 <212> PRT <213> Enterococcus casseliflavus <400> 24 Met Glu His Thr Tyr Met Leu Ile Asn Met Lys Gln Leu Leu Thr Val   1 5 10 15 Ala Lys Glu Asn Gln Phe Ala Val Gly Ala Phe Asn Val Ala Asp Ser              20 25 30 Asn Phe Leu Arg Val Val Ile Glu Ala Ala Glu Glu Asn Asp Ala Pro          35 40 45 Ala Ile Ale Ile His Pro Thr Glu Leu Ala Phe Thr Lys Asp Glu      50 55 60 Phe Phe His Tyr Ala Val Ser Arg Ala Lys Asn Ser Ser Val Pro Ile  65 70 75 80 Val Ile His Met Asp His Gly Asp Ser Leu Ala Ser Ile Met Arg Ala                  85 90 95 Ile His Cys Gly Phe Ser Ser Val Met Ile Asp Gly Ser Leu Leu Pro             100 105 110 Phe Glu Glu Asn Ile Ala Leu Thr Lys Gln Ile Thr Glu Ile Cys His         115 120 125 Pro Ile Gly Val Ser Val Glu Gly Glu Leu Gly Thr Ile Gly Asn Thr     130 135 140 Gly Thr Ser Val Glu Gly Gly Val Ser Glu Val Ile Tyr Thr Arg Pro 145 150 155 160 Glu Asp Ala Gln Glu Phe Ile Glu Lys Thr Gly Ile Asp Thr Leu Ala                 165 170 175 Val Ala Ile Gly Thr Ala His Gly Ile Tyr Pro Lys Asp Ile Lys Pro             180 185 190 Glu Leu Lys Met Asp Val Leu Gln Glu Ile Lys Asp Arg Val Asp Ile         195 200 205 Pro Leu Val Leu His Gly Gly Ser Ala Asn Pro Asp Glu Glu Ile Ala     210 215 220 Gln Ala Val Ala Ile Gly Ile Gln Lys Val Asn Ile Ser Ser Asp Tyr 225 230 235 240 Lys Tyr Ala Phe Tyr Lys Lys Cys Arg Gln Ile Leu Ala Glu Thr Glu                 245 250 255 Leu Trp Asp Pro Asn Ala Ile Tyr Pro Glu Cys Ile Glu Ala Ala Lys             260 265 270 Ala Val Val Thr Gln Lys Met Thr Leu Phe Asn Ala Ile Gly Lys Ala         275 280 285 Ala Leu Tyr Arg Gln Gln Asn Ile Gln Pro Trp Arg Ser Glu Gln Asn     290 295 300 <210> 25 <211> 289 <212> PRT <213> Enterococcus faecalis <400> 25 Met Pro Val Val Ser Gly Ala Glu Phe Leu Lys Ala Ala Arg Lys Gly   1 5 10 15 Gly Tyr Ala Val Gly Gly Tyr Asn Thr Asn Asn Leu Glu Trp Thr Gln              20 25 30 Ala Ile Leu Glu Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln          35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Val Ala Lys      50 55 60 Asp Met Ile Thr Asn Leu Val Asp Ser Met Asn Ile Thr Val  65 70 75 80 Ala Ile His Leu Asp His Gly Asp Tyr Glu Ala Ala Leu Glu Cys Ile                  85 90 95 Glu Val Gly Tyr Thr Ser Ile Met Phe Asp Gly Ser His Leu Pro Phe             100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Asp Val Val Glu Lys Ala His Ala         115 120 125 Lys Gly Ile Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu     130 135 140 Asp Gly Ile Ile Gly Thr Gly Glu Leu Ala Asp Ile Glu Glu Cys Lys 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn                 165 170 175 Ile His Gly Gln Tyr Pro Glu Asn Trp Lys Gly Leu Ala Phe Asp His             180 185 190 Leu Gln Ala Ile Ala Glu Ala Val Gly Ser Asp Val Pro Leu Val Leu         195 200 205 His Gly Gly Ser Gly Ile Pro Glu Glu Glu Ile Glu Lys Ala Ile Ser     210 215 220 Met Gly Ile Ser Lys Val Asn Val Asn Thr Glu Phe Gln Leu Ser Phe 225 230 235 240 Ala Lys Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Leu Glu Gly                 245 250 255 Lys Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Lys Ala Ala Ile             260 265 270 Ile Lys Asp Ala Glu Glu His Ile Asp Trp Phe Gly Ser Ala Asn Lys         275 280 285 Ala     <210> 26 <211> 289 <212> PRT <213> Enterococcus faecium <400> 26 Met Tyr Thr Thr Leu Lys Glu Val Thr Lys Thr Ala Glu Glu Leu Asn   1 5 10 15 Phe Thr Val Gly Ala Phe Asn Thr His Asn Leu Glu Met Leu Pro Ala              20 25 30 Met Leu Gly Ala Ala Lys Glu Met Gly Ser Pro Ile Ile Ile Gln Thr          35 40 45 Ser Val Asp Thr Ala Lys Tyr Ile Gly Met His Val Val Leu Val Asn Val      50 55 60 Met Thr Ala Ile Ala Glu Glu Glu Met Val Asp Ala Val Leu His Leu  65 70 75 80 Asp His Ala Arg Asp Phe Asp Asp Ile Lys Glu Ala Ile Asp Ser Gly                  85 90 95 Tyr Thr Ser Val Met Tyr Asp Gly Ser His Leu Pro Phe Lys Glu Asn             100 105 110 Ile Leu Lys Thr Lys Ala Val Val Glu Tyr Ala His Ala His Gly Val         115 120 125 Ser Val Glu Gly Glu Leu Gly Thr Ile Gly Gly Gly Thr Glu Glu Gly Ile     130 135 140 His Val Ala Glu Asn Asp Lys Val Tyr Thr Arg Pro Glu Asp Ala Val 145 150 155 160 Lys Phe Val Glu Ala Thr Gly Val Asp Ala Leu Ala Ile Ala Ile Gly                 165 170 175 Thr Asn His Gly Gln Phe Lys Ser Lys Thr Glu Val Asn Ile Pro Leu             180 185 190 Leu Lys Glu Ile Asp Ala Val Val Asp Val Pro Leu Val Ile His Gly         195 200 205 Gly Thr Gly Val Lys Glu Glu Asp Tyr Pro Glu Leu Ile Asn Asn Gly     210 215 220 Ile Arg Lys Phe Asn Val Gly Thr Glu Leu Leu Val Asn Trp Thr Lys 225 230 235 240 Val Ala Lys Glu Thr Phe Ser Glu Thr Glu Ile Asn Lys Ser Leu Arg                 245 250 255 His Asn Val Ile Pro Ala Asn Glu Ala Val Lys Glu Ile Val Lys His             260 265 270 Lys Ile Gly Leu Phe Met Asn Leu Glu Ser Pro Ile Asn Leu Gly Asn         275 280 285 Lys     <210> 27 <211> 289 <212> PRT <213> Enterococcus flavescens <400> 27 Met Pro Val Val Ser Gly Ala Glu Phe Leu Lys Ala Ala Arg Gln Gly   1 5 10 15 Gly Tyr Ala Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Gln              20 25 30 Ala Ile Leu Lys Ala Ala Glu Ala Lys Lys Ala Pro Val Leu Leu Gln          35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Val Ala Lys      50 55 60 Asp Ile Val Thr Asn Leu Val Asp Ser Met Asn Ile Thr Val Pro Val  65 70 75 80 Ala Ile His Leu Asp His Gly Asp Tyr Asp Ala Ala Met Glu Cys Ile                  85 90 95 Glu Val Gly Tyr Thr Ser Val Met Phe Asp Gly Ser His Leu Pro Phe             100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Glu Val Val Glu Lys Ala His Ala         115 120 125 Lys Gly Val Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu     130 135 140 Asp Gly Ile Ile Gly Ser Gly Glu Leu Ala Asp Ile Glu Glu Cys Lys 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn                 165 170 175 Ile His Gly Gln Tyr Pro Glu Asn Trp Lys Gly Leu Ala Phe Asp His             180 185 190 Leu Gln Ala Ile Ala Asp Ala Val Gly Ser Asp Val Pro Leu Val Leu         195 200 205 His Gly Gly Ser Gly Ile Pro Gln Asp Gln Ile Glu Lys Ala Ile Ser     210 215 220 Met Gly Val Ser Lys Val Asn Val Asn Thr Glu Phe Gln Leu Ser Phe 225 230 235 240 Ala Lys Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Lys Glu Gly                 245 250 255 Lys Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Val Ala Ala Ile             260 265 270 Val Lys Asp Ala Glu Glu His Ile Asp Trp Phe Gly Ser Ala Gly Lys         275 280 285 Ala     <210> 28 <211> 289 <212> PRT <213> Enterococcus gilvus <400> 28 Met Pro Val Val Ser Gly Ala Glu Phe Leu Lys Ala Ala Arg Lys Gly   1 5 10 15 Gly Tyr Ala Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Gln              20 25 30 Ala Ile Leu Lys Ala Ala Glu Ala Lys Ays Pro Val Leu Ile Gln          35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Val Ala Lys      50 55 60 Asp Ile Ile Cys Asn Leu Val Asp Ser Met Gly Ile Thr Val Val  65 70 75 80 Ala Ile His Leu Asp His Gly Asp Tyr Glu Ala Ala Leu Glu Cys Ile                  85 90 95 Glu Val Gly Tyr Thr Ser Ile Met Phe Asp Gly Ser His Leu Pro Phe             100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Asp Val Val Glu Lys Ala His Ala         115 120 125 Lys Gly Ile Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu     130 135 140 Asp Gly Ile Gly Aly Gly Glu Leu Ala Asp Val Asp Glu Cys Val 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn                 165 170 175 Ile His Gly Val Tyr Pro Glu Asn Trp Lys Gly Leu Ala Phe Asp His             180 185 190 Leu Gln Glu Ile Ala Asn Ala Val Gly Ser Asp Val Pro Leu Val Leu         195 200 205 His Gly Gly Ser Gly Ile Pro Gln Glu Glu Ile Gln Lys Ala Ile Ser     210 215 220 Met Gly Val Ser Lys Val Asn Val Asn Thr Glu Phe Gln Leu Ser Phe 225 230 235 240 Ala Lys Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Lys Glu Gly                 245 250 255 Lys Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Val Ala Ala Ile             260 265 270 Val Lys Asp Ala Glu Glu His Ile Asp Trp Phe Gly Ser Ala Asn Lys         275 280 285 Ala     <210> 29 <211> 288 <212> PRT <213> Enterococcus haemoperoxidus <400> 29 Met Pro Leu Val Ser Gly Thr Glu Phe Leu Lys Ala Ala Arg Lys Gly   1 5 10 15 Gly Tyr Gly Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Lys              20 25 30 Ala Ile Leu Glu Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln          35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Gln Val Cys Tyr      50 55 60 Asp Leu Val Lys Asp Leu Ile Asp Ser Met Gly Ile Thr Val Val  65 70 75 80 Ala Leu His Leu Asp His Gly Glu Tyr Glu Asp Ala Leu Glu Cys Ile                  85 90 95 Glu Ile Gly Tyr Thr Ser Val Met Phe Asp Gly Ser His Leu Pro Phe             100 105 110 Glu Glu Asn Ile Glu Lys Ala Lys Asp Val Val Ala Lys Ala His Ala         115 120 125 Lys Gly Val Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu     130 135 140 Asp Gly Ile Ile Gly Ser Gly Glu Leu Ala Asp Val Gln Glu Cys Val 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn                 165 170 175 Ile His Gly Ser Tyr Pro Asp Asn Trp Thr Gly Leu Ala Phe Asp His             180 185 190 Leu Gln Glu Ile Ala Asp Ala Val Gly Asp Ile Pro Leu Val Leu His         195 200 205 Gly Gly Ser Gly Ile Pro Leu Glu Gln Val Gln Lys Ala Ile Ser Met     210 215 220 Gly Val Ser Lys Ile Asn Val Asn Thr Glu Cys Gln Glu Val Phe Ala 225 230 235 240 Ala Ala Thr Arg Lys Tyr Ile Glu Glu Gly Lys Asp Leu Glu Gly Lys                 245 250 255 Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Thr Lys Ala Val Thr             260 265 270 Glu Leu Val Glu Gln Arg Ile Glu Trp Phe Gly Ser Ala Asn Lys Ala         275 280 285 <210> 30 <211> 290 <212> PRT <213> Enterococcus italicus <400> 30 Met Pro Leu Val Ser Gly Thr Glu Ile Phe Lys Ala Ala Arg Lys Gly   1 5 10 15 Gly Tyr Ala Val Gly Ala Phe Asn Thr Asn Asn Leu Glu Trp Thr Gln              20 25 30 Ala Ile Leu Glu Ala Ala Glu Ala Lys Gln Thr Pro Val Leu Ile Gln          35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Leu Val Lys      50 55 60 Asp Met Val Thr Asp Leu Ile Glu Ser Leu Asn Ile Thr Val Pro Val  65 70 75 80 Val Leu His Leu Asp His Gly His Tyr Glu Asp Ala Leu Glu Cys Ile                  85 90 95 Glu Val Gly Tyr Thr Ser Val Met Phe Asp Gly Ser His Leu Pro Phe             100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Glu Val Val Ala Lys Ala His Ala         115 120 125 Lys Gly Val Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu     130 135 140 Asp Gly Ile Gly Aly Gly Glu Leu Ala Asp Val Asp Glu Cys Val 145 150 155 160 Gln Met Val Asn Thr Gly Ile Asp Phe Leu Ala Cys Gly Ile Gly Asn                 165 170 175 Ile His Gly Lys Tyr Pro Glu Asn Trp Lys Gly Leu Ala Phe Asp His             180 185 190 Leu Gln Ala Ile Ala Asp Ala Val Gly Ser Asp Val Pro Leu Val Leu         195 200 205 His Gly Gly Ser Gly Ile Pro Gln Asp Gln Ile Glu Lys Ala Ile Ser     210 215 220 Met Gly Ile Ser Lys Val Asn Val Asn Thr Glu Phe Gln Leu Ser Phe 225 230 235 240 Ala Lys Ala Ile Arg Glu Tyr Ile Glu Ala Gly Lys Asp Leu Glu Gly                 245 250 255 Lys Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Lys Ala Ala Ile             260 265 270 Ile Lys Asp Cys Glu Glu His Ile Asp Trp Phe Gly Ser Ala Asn Lys         275 280 285 Ala Lys     290 <210> 31 <211> 288 <212> PRT <213> Enterococcus moraviensis <400> 31 Met Pro Leu Val Ser Gly Thr Glu Phe Leu Lys Ala Ala Arg Lys Gly   1 5 10 15 Gly Tyr Gly Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Lys              20 25 30 Ala Ile Leu Glu Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln          35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Gln Val Cys Tyr      50 55 60 Asp Leu Val Lys Asp Leu Ile Asp Ser Met Gly Ile Thr Val Val  65 70 75 80 Ala Leu His Leu Asp His Gly Glu Tyr Glu Asp Ala Leu Glu Cys Ile                  85 90 95 Glu Ile Gly Tyr Thr Ser Val Met Phe Asp Gly Ser His Leu Pro Phe             100 105 110 Glu Glu Asn Ile Glu Lys Ala Lys Asp Val Val Ala Lys Ala His Ala         115 120 125 Lys Gly Val Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu     130 135 140 Asp Gly Ile Ile Gly Ser Gly Glu Leu Ala Asp Val Gln Glu Cys Val 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn                 165 170 175 Ile His Gly Ser Tyr Pro Glu Asn Trp Thr Gly Leu Ala Phe Asp His             180 185 190 Leu Gln Glu Ile Ala Asp Ala Val Gly Asp Ile Pro Leu Val Leu His         195 200 205 Gly Gly Ser Gly Ile Pro Leu Glu Gln Val Gln Lys Ala Ile Ser Met     210 215 220 Gly Val Ser Lys Ile Asn Val Asn Thr Glu Cys Gln Glu Val Phe Ala 225 230 235 240 Ala Ala Thr Arg Lys Tyr Ile Glu Glu Gly Lys Asp Leu Glu Gly Lys                 245 250 255 Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Thr Lys Ala Val Thr             260 265 270 Glu Leu Val Glu Gln Arg Ile Glu Trp Phe Gly Ser Ala Asn Lys Ala         275 280 285 <210> 32 <211> 289 <212> PRT <213> Enterococcus mundtii <400> 32 Met Pro Val Val Ser Gly Ala Glu Phe Leu Gln Ala Ala Arg Lys Gly   1 5 10 15 Gly Tyr Ala Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Gln              20 25 30 Ala Ile Leu Glu Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln          35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Val Cys Lys      50 55 60 Asp Met Ile Thr Asn Leu Val Glu Ser Met Asn Ile Thr Val Val  65 70 75 80 Ala Ile His Leu Asp His Gly Asp Tyr Asp Ala Ala Leu Glu Cys Ile                  85 90 95 Glu Val Gly Tyr Thr Ser Val Met Phe Asp Gly Ser His Leu Pro Phe             100 105 110 Glu Glu Asn Leu Lys Leu Ala Arg Asp Val Val Glu Lys Ala His Ala         115 120 125 Lys Gly Ile Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu     130 135 140 Asp Gly Ile Ile Gly Thr Gly Glu Leu Ala Asp Ile Glu Glu Cys Lys 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn                 165 170 175 Ile His Gly Gln Tyr Pro Glu Asn Trp Ser Gly Leu Ala Phe Asp His             180 185 190 Leu Gln Ala Ile Ala Asp Ala Val Gly Ser Asp Val Pro Leu Val Leu         195 200 205 His Gly Gly Ser Gly Ile Pro Gln Asp Gln Ile Glu Lys Ala Ile Ser     210 215 220 Met Gly Ile Ser Lys Val Asn Val Asn Thr Glu Phe Gln Leu Ser Phe 225 230 235 240 Ala Ala Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Arg Glu Gly                 245 250 255 Lys Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Lys Ala Ala Ile             260 265 270 Val Lys Asp Ala Glu Ser His Ile Asp Trp Phe Gly Ser Ala Asn Lys         275 280 285 Ala     <210> 33 <211> 289 <212> PRT <213> Enterococcus pallens <400> 33 Met Pro Val Val Ser Gly Ala Glu Phe Leu Lys Ala Ala Arg Gln Gly   1 5 10 15 Gly Tyr Ala Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Gln              20 25 30 Ala Ile Leu Arg Ala Ala Gla Ala Lys Ala Pro Val Leu Ile Gln          35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Val Ala Lys      50 55 60 Asp Ile Ile Cys Asp Leu Val Glu Ser Met Asp Ile Thr Val Pro Val  65 70 75 80 Ala Ile His Leu Asp His Gly Ser Tyr Glu Ala Ala Leu Glu Cys Ile                  85 90 95 Glu Val Gly Tyr Thr Ser Val Met Phe Asp Gly Ser His Leu Pro Phe             100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Asp Val Val Glu Lys Ala His Ala         115 120 125 Lys Gly Val Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu     130 135 140 Asp Gly Ile Gly Aly Gly Glu Leu Ala Asp Ile Asp Glu Cys Val 145 150 155 160 Gln Met Val Gly Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn                 165 170 175 Ile His Gly Val Tyr Pro Glu Asn Trp Lys Gly Leu Ala Phe Asp His             180 185 190 Leu Gln Ala Ile Ala Asp Ala Val Gly Ser Glu Val Pro Leu Val Leu         195 200 205 His Gly Gly Ser Gly Ile Pro Gln Glu Glu Ile Gln Lys Ala Ile Ser     210 215 220 Met Gly Val Ser Lys Val Asn Val Asn Thr Glu Phe Gln Leu Ser Phe 225 230 235 240 Ala Lys Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Lys Glu Gly                 245 250 255 Lys Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Val Ala Ala Ile             260 265 270 Val Lys Asp Ala Glu Glu His Ile Asp Trp Phe Gly Ser Ala Asn Lys         275 280 285 Ala     <210> 34 <211> 288 <212> PRT <213> Enterococcus phoeniculicola <400> 34 Met Pro Leu Val Ser Gly Thr Glu Phe Leu Lys Ala Ala His Lys Gly   1 5 10 15 Gly Tyr Gly Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Lys              20 25 30 Ala Ile Leu Glu Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln          35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Val Cys Tyr      50 55 60 Asp Leu Val Lys Asp Leu Val Asp Ser Met Gly Ile Thr Val Val  65 70 75 80 Ala Ile His Leu Asp His Gly Glu Tyr Glu Asp Ala Leu Glu Cys Ile                  85 90 95 Glu Ile Gly Tyr Thr Ser Val Met Phe Asp Gly Ser His Leu Pro Phe             100 105 110 Glu Glu Asn Ile Glu Lys Ala Lys Asp Val Val Ala Lys Ala His Ala         115 120 125 Lys Gly Val Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu     130 135 140 Asp Gly Ile Ile Gly Ser Gly Glu Leu Ala Asp Ile Glu Glu Cys Lys 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn                 165 170 175 Ile His Gly Ser Tyr Pro Asp Asn Trp Thr Gly Leu Ala Phe Asp His             180 185 190 Leu Gln Ala Ile Ser Asp Ala Val Gly Asp Ile Pro Leu Val Leu His         195 200 205 Gly Gly Ser Gly Ile Pro Leu Glu Gln Val Gln Lys Ala Ile Ser Met     210 215 220 Gly Val Ser Lys Ile Asn Val Asn Thr Glu Cys Gln Glu Val Phe Ala 225 230 235 240 Ala Ala Thr Arg Lys Tyr Ile Glu Glu Gly Lys Asp Leu Glu Gly Lys                 245 250 255 Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Thr Lys Ala Val Thr             260 265 270 Glu Leu Val Glu Gln Arg Ile Glu Trp Phe Gly Ser Thr Asn Lys Ala         275 280 285 <210> 35 <211> 289 <212> PRT <213> Enterococcus raffinosus <400> 35 Met Pro Val Val Ser Gly Ala Glu Phe Leu Lys Ala Ala Arg Lys Gly   1 5 10 15 Gly Tyr Ala Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Gln              20 25 30 Ala Ile Leu Lys Ala Ala Glu Ala Lys Ays Pro Val Leu Ile Gln          35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Val Ala Lys      50 55 60 Asp Ile Ile Thr Asp Leu Val Asp Ser Met Gly Ile Thr Val Val  65 70 75 80 Ala Ile His Leu Asp His Gly Asp Tyr Glu Ala Ala Leu Glu Cys Ile                  85 90 95 Glu Val Gly Tyr Thr Ser Ile Met Phe Asp Gly Ser His Leu Pro Phe             100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Asp Val Val Glu Lys Ala His Ala         115 120 125 Lys Gly Ile Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu     130 135 140 Asp Gly Ile Gly Aly Gly Glu Leu Ala Asp Ile Asp Glu Cys Val 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn                 165 170 175 Ile His Gly Val Tyr Pro Glu Asn Trp Lys Gly Leu Ala Phe Asp His             180 185 190 Leu Gln Ala Ile Ala Asp Ala Val Gly Asn Asp Val Pro Leu Val Leu         195 200 205 His Gly Gly Ser Gly Ile Pro Gln Glu Glu Ile Gln Lys Ala Ile Ser     210 215 220 Met Gly Val Ser Lys Val Asn Val Asn Thr Glu Phe Gln Leu Ser Phe 225 230 235 240 Ala Lys Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Lys Glu Gly                 245 250 255 Lys Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Val Ala Ala Ile             260 265 270 Val Lys Asp Ala Glu Glu His Ile Asp Trp Phe Gly Ser Ala Asn Lys         275 280 285 Ala     <210> 36 <211> 289 <212> PRT <213> Enterococcus saccharolyticus <400> 36 Met Pro Val Val Ser Gly Ala Glu Phe Leu Lys Ala Ala Arg Gln Gly   1 5 10 15 Gly Tyr Ala Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Gln              20 25 30 Ala Ile Leu Glu Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln          35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Leu Ala Lys      50 55 60 Asp Leu Ile Cys Asp Leu Val Glu Ser Met Asn Ile Thr Val Pro Val  65 70 75 80 Ala Ile His Leu Asp His Gly Asp Tyr Asp Ala Ala Met Glu Cys Ile                  85 90 95 Glu Val Gly Tyr Thr Ser Val Met Phe Asp Gly Ser His Leu Pro Phe             100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Glu Val Val Glu Lys Ala His Ala         115 120 125 Lys Gly Val Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu     130 135 140 Asp Gly Ile Ile Gly Ala Gly Glu Leu Ala Asp Ile Glu Glu Cys Lys 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn                 165 170 175 Ile His Gly Gln Tyr Pro Glu Asn Trp Gln Gly Leu Ala Phe Asp His             180 185 190 Leu Gln Ala Ile Ala Asp Ala Val Gly Ser Asp Val Pro Leu Val Leu         195 200 205 His Gly Gly Ser Gly Ile Pro Glu Glu Glu Ile Glu Lys Ala Ile Ser     210 215 220 Met Gly Val Ser Lys Val Asn Val Asn Thr Glu Phe Gln Leu Ser Phe 225 230 235 240 Ala Lys Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Gln Glu Gly                 245 250 255 Lys Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Lys Ala Ala Ile             260 265 270 Ile Lys Asp Ala Glu Glu His Ile Asp Trp Phe Gly Ser Ala Gly Lys         275 280 285 Ala     <210> 37 <211> 289 <212> PRT <213> Enterococcus sp. <400> 37 Met Pro Val Val Ser Gly Ala Glu Phe Leu Lys Ala Ala Arg Gln Gly   1 5 10 15 Gly Tyr Ala Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Gln              20 25 30 Ala Ile Leu Lys Ala Ala Glu Ala Lys Ays Pro Val Leu Ile Gln          35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Val Ala Lys      50 55 60 Asp Ile Val Thr Asn Leu Val Asp Ser Met Asn Ile Thr Val Pro Val  65 70 75 80 Ala Ile His Leu Asp His Gly Asp Tyr Asp Ala Ala Met Glu Cys Ile                  85 90 95 Glu Val Gly Tyr Thr Ser Val Met Phe Asp Gly Ser His Leu Pro Phe             100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Glu Val Val Glu Lys Ala His Ala         115 120 125 Lys Gly Val Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu     130 135 140 Asp Gly Ile Ile Gly Ser Gly Glu Leu Ala Asp Ile Glu Glu Cys Lys 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn                 165 170 175 Ile His Gly Gln Tyr Pro Glu Asn Trp Lys Gly Leu Ala Phe Asp His             180 185 190 Leu Gln Ala Ile Ala Asp Ala Val Gly Ser Asp Val Pro Leu Val Leu         195 200 205 His Gly Gly Ser Gly Ile Pro Gln Asp Gln Ile Glu Lys Ala Ile Ser     210 215 220 Met Gly Val Ser Lys Val Asn Val Asn Thr Glu Phe Gln Leu Ser Phe 225 230 235 240 Ala Lys Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Lys Glu Gly                 245 250 255 Lys Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Val Ala Ala Ile             260 265 270 Val Lys Asp Ala Glu Glu His Ile Asp Trp Phe Gly Ser Ala Gly Lys         275 280 285 Ala     <210> 38 <211> 289 <212> PRT <213> Enterococcus villorum <400> 38 Met Pro Val Val Ser Gly Ala Glu Phe Leu Lys Ala Ala Arg Lys Gly   1 5 10 15 Gly Tyr Ala Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Gln              20 25 30 Ala Ile Leu Glu Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln          35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Val Ala Lys      50 55 60 Asp Ile Ile Cys Asp Leu Val Glu Ser Met Asn Ile Thr Val Pro Val  65 70 75 80 Ala Ile His Leu Asp His Gly Asp Tyr Asp Ala Ala Met Glu Cys Ile                  85 90 95 Glu Val Gly Tyr Thr Ser Val Met Phe Asp Gly Ser His Leu Pro Phe             100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Glu Val Val Glu Lys Ala His Ala         115 120 125 Lys Gly Ile Ser Val Glu Cys Glu Val Gly Ser Ile Gly Gly Glu Glu     130 135 140 Asp Gly Ile Ile Gly Ser Gly Glu Leu Ala Asp Ile Glu Glu Cys Lys 145 150 155 160 Gln Met Val Ala Thr Gly Ile Asp Tyr Leu Ala Cys Gly Ile Gly Asn                 165 170 175 Ile His Gly Gln Tyr Pro Glu Asn Trp Ala Gly Leu Ala Phe Asp His             180 185 190 Leu Gln Ala Ile Ala Asp Ala Val Gly Ser Asp Val Pro Leu Val Leu         195 200 205 His Gly Gly Ser Gly Ile Pro Gln Glu Glu Ile Gln Lys Ala Ile Ser     210 215 220 Met Gly Val Ser Lys Val Asn Val Asn Thr Glu Phe Gln Leu Ser Phe 225 230 235 240 Ala Lys Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Lys Glu Gly                 245 250 255 Lys Gly Phe Asp Pro Arg Lys Leu Leu Ala Pro Gly Lys Ala Ala Ile             260 265 270 Ile Lys Asp Ala Glu Glu His Ile Asp Trp Phe Gly Ser Ala Asn Lys         275 280 285 Ala     <210> 39 <211> 350 <212> PRT <213> Escherichia coli <400> 39 Met Thr Asp Ile Ala Gln Leu Leu Gly Lys Asp Ala Asp Asn Leu Leu   1 5 10 15 Gln His Arg Cys Met Thr Ile Pro Ser Asp Gln Leu Tyr Leu Pro Gly              20 25 30 His Asp Tyr Val Asp Arg Val Met Ile Asp Asn Asn Arg Pro Pro Ala          35 40 45 Val Leu Arg Asn Met Gln Thr Leu Tyr Asn Thr Gly Arg Leu Ala Gly      50 55 60 Thr Gly Tyr Leu Ser Ile Leu Pro Val Asp Gln Gly Val Glu His Ser  65 70 75 80 Ala Gly Ala Ser Phe Ala Ala Asn Pro Leu Tyr Phe Asp Pro Lys Asn                  85 90 95 Ile Val Glu Leu Ala Ile Glu Ala Gly Cys Asn Cys Val Ala Ser Thr             100 105 110 Tyr Gly Val Leu Ala Ser Val Ser Arg Arg Tyr Ala His Arg Ile Pro         115 120 125 Phe Leu Val Lys Leu Asn His Asn Glu Thr Leu Ser Tyr Pro Asn Thr     130 135 140 Tyr Asp Gln Thr Leu Tyr Ala Ser Val Glu Gln Ala Phe Asn Met Gly 145 150 155 160 Ala Val Ala Val Gly Ala Thr Ile Tyr Phe Gly Ser Glu Glu Ser Arg                 165 170 175 Arg Gln Ile Glu Glu Ile Ser Ala Ala Phe Glu Arg Ala His Glu Leu             180 185 190 Gly Met Val Thr Val Leu Trp Ala Tyr Leu Arg Asn Ser Ala Phe Lys         195 200 205 Lys Asp Gly Val Asp Tyr His Val Ser Ala Asp Leu Thr Gly Gln Ala     210 215 220 Asn His Leu Ala Ala Thr Ile Gly Ala Asp Ile Val Lys Gln Lys Met 225 230 235 240 Ala Glu Asn Asn Gly Gly Tyr Lys Ala Ile Asn Tyr Gly Tyr Thr Asp                 245 250 255 Asp Arg Val Tyr Ser Lys Leu Thr Ser Glu Asn Pro Ile Asp Leu Val             260 265 270 Arg Tyr Gln Leu Ala Asn Cys Tyr Met Gly Arg Ala Gly Leu Ile Asn         275 280 285 Ser Gly Gly Ala Gly Gly Glu Thr Asp Leu Ser Asp Ala Val Arg     290 295 300 Thr Ala Val Ile Asn Lys Arg Ala Gly Gly Met Gly Leu Ile Leu Gly 305 310 315 320 Arg Lys Ala Phe Lys Lys Ser Ale Asp Gly Val Lys Leu Ile Asn                 325 330 335 Ala Val Gln Asp Val Tyr Leu Asp Ser Lys Ile Thr Ile Ala             340 345 350 <210> 40 <211> 359 <212> PRT <213> Escherichia coli <400> 40 Met Ser Lys Ile Phe Asp Phe Val Lys Pro Gly Val Ile Thr Gly Asp   1 5 10 15 Asp Val Gln Lys Val Phe Gln Val Ala Lys Glu Asn Asn Phe Ala Leu              20 25 30 Pro Ala Val Asn Cys Val Gly Thr Asp Ser Ile Asn Ala Val Leu Glu          35 40 45 Thr Ala Lys Val Lys Ala Pro Val Ile Val Gln Phe Ser Asn Gly      50 55 60 Gly Ala Ser Phe Ile Ala Gly Lys Gly Val Lys Ser Asp Val Pro Gln  65 70 75 80 Gly Ala Ala Ile Leu Gly Ala Ile Ser Gly Ala His His Val His Gln                  85 90 95 Met Ala Glu His Tyr Gly Val Pro Val Ile Leu His Thr Asp His Cys             100 105 110 Ala Lys Lys Leu Leu Pro Trp Ile Asp Gly Leu Leu Asp Ala Gly Glu         115 120 125 Lys His Phe Ala Ala Thr Gly Lys Pro Leu Phe Ser Ser His Met Ile     130 135 140 Asp Leu Ser Glu Glu Ser Leu Gln Glu Asn Ile Glu Ile Cys Ser Lys 145 150 155 160 Tyr Leu Glu Arg Met Ser Lys Ile Gly Met Thr Leu Glu Ile Glu Leu                 165 170 175 Gly Cys Thr Gly Gly Glu Glu Asp Gly Val Asp Asn Ser His Met Asp             180 185 190 Ala Ser Ala Leu Tyr Thr Gln Pro Glu Asp Val Asp Tyr Ala Tyr Thr         195 200 205 Glu Leu Ser Lys Ile Ser Pro Arg Phe Thr Ile Ala Ala Ser Phe Gly     210 215 220 Asn Val His Gly Val Tyr Lys Pro Gly Asn Val Val Leu Thr Pro Thr 225 230 235 240 Ile Leu Arg Asp Ser Gln Glu Tyr Val Ser Lys Lys His Asn Leu Pro                 245 250 255 His Asn Ser Leu Asn Phe Val Phe His Gly Gly Ser Gly Ser Thr Ala             260 265 270 Gln Glu Ile Lys Asp Ser Val Ser Tyr Gly Val Val Lys Met Asn Ile         275 280 285 Asp Thr Asp Thr Gln Trp Ala Thr Trp Glu Gly Val Leu Asn Tyr Tyr     290 295 300 Lys Ala Asn Glu Ala Tyr Leu Gln Gly Gln Leu Gly Asn Pro Lys Gly 305 310 315 320 Glu Asp Gln Pro Asn Lys Lys Tyr Tyr Asp Pro Arg Val Trp Leu Arg                 325 330 335 Ala Gly Gln Thr Ser Met Ile Ala Arg Leu Glu Lys Ala Phe Gln Glu             340 345 350 Leu Asn Ale Ile Asp Val Leu         355 <210> 41 <211> 387 <212> PRT <213> Escherichia sp. <400> 41 Met Ile Gly Val Ser Glu Arg Arg Arg Ser Thr Cys Asn Leu Lys Tyr   1 5 10 15 Asp Glu Tyr Lys Ala Arg Arg Tyr Arg Thr Arg Asp Met Ser Lys Ile              20 25 30 Phe Asp Phe Val Lys Pro Gly Val Ile Thr Gly Asp Asp Val Gln Lys          35 40 45 Val Phe Gln Val Ala Lys Glu Asn Asn Phe Ala Leu Pro Ala Val Asn      50 55 60 Cys Val Gly Thr Asp Ser Ile Asn Ala Val Leu Glu Thr Ala Ala Lys  65 70 75 80 Val Lys Ala Pro Val Ile Val Gln Phe Ser Asn Gly Gly Ala Ser Phe                  85 90 95 Ile Ala Gly Lys Gly Val Lys Ser Asp Val Pro Gln Gly Ala Ala Ile             100 105 110 Leu Gly Ala Ile Ser Gly Ala His His Val His Gln Met Ala Glu His         115 120 125 Tyr Gly Val Val Ile Leu His Thr Asp His Cys Ala Lys Lys Leu     130 135 140 Leu Pro Trp Ile Asp Gly Leu Leu Asp Ala Gly Glu Lys His Phe Ala 145 150 155 160 Ala Thr Gly Lys Pro Leu Phe Ser Ser His Met Ile Asp Leu Ser Glu                 165 170 175 Glu Ser Leu Gln Glu Asn Ile Glu Ile Cys Ser Lys Tyr Leu Glu Arg             180 185 190 Met Ser Lys Ile Gly Met Thr Leu Glu Ile Glu Leu Gly Cys Thr Gly         195 200 205 Gly Glu Glu Asp Gly Val Asp Asn Ser Met Met Asp Ala Ser Ala Leu     210 215 220 Tyr Thr Gln Pro Glu Asp Val Asp Tyr Ala Tyr Thr Glu Leu Ser Lys 225 230 235 240 Ile Ser Pro Arg Phe Thr Ile Ala Ala Ser Phe Gly Asn Val His Gly                 245 250 255 Val Tyr Lys Pro Gly Asn Val Val Leu Thr Pro Thr Ile Leu Arg Asp             260 265 270 Ser Gln Glu Tyr Val Ser Lys Lys His Asn Leu Pro His Asn Ser Leu         275 280 285 Asn Phe Val Phe His Gly Gly Ser Gly Ser Thr Ala Gln Glu Ile Lys     290 295 300 Asp Ser Val Ser Tyr Gly Val Val Lys Met Asn Ile Asp Thr Asp Thr 305 310 315 320 Gln Trp Ala Thr Trp Glu Gly Val Leu Asn Tyr Tyr Lys Glu Asn Glu                 325 330 335 Ala Tyr Leu Gln Gly Gln Leu Gly Asn Pro Lys Gly Glu Asp Gln Pro             340 345 350 Asn Lys Lys Tyr Tyr Asp Pro Arg Val Trp Leu Arg Ala Gly Gln Thr         355 360 365 Ser Met Ile Ala Arg Leu Glu Lys Ala Phe Gln Glu Leu Asn Ala Ile     370 375 380 Asp Val Leu 385 <210> 42 <211> 295 <212> PRT <213> Geobacillus kaustophilus <400> 42 Met Pro Leu Val Ser Met Lys Asn Met Leu Gln Arg Ala Trp Gln Gly   1 5 10 15 Arg Tyr Ala Val Gly His Phe Asn Ile Asn Asn Leu Glu Phe Ala Gln              20 25 30 Ala Ile Leu Glu Ala Glu Glu Glu Glu Glu Ala Pro Val Ile Leu Ala          35 40 45 Val Ser Pro Gly Tyr Ile Asp His Leu Gly Gly Leu Arg Thr Ala Ala      50 55 60 Ala Met Val Lys Glu Leu Val Lys Glu Tyr Arg Ile Thr Val Pro Val  65 70 75 80 Ala Leu His Leu Asp His Gly Ser Ser Tyr Glu Gln Cys Leu Glu Ala                  85 90 95 Met Glu Ala Gly Phe Thr Ser Val Met Ile Asp Ala Ser His His Pro             100 105 110 Leu Glu Gln Asn Ile Ser Ile Thr Lys Gln Val Val Lys Ala Ala Arg         115 120 125 Glu Phe Gly Val Ser Val Glu Ala Glu Val Gly Arg Ile Gly Gly Gln     130 135 140 Glu Asp Asp Leu Val Val Asp Glu Ala Glu Ala Met Tyr Ala Ile Pro 145 150 155 160 Glu Glu Cys Glu Arg Leu Val Lys Glu Thr Gly Val Asp Cys Leu Ala                 165 170 175 Pro Ala Leu Gly Ser Val His Gly Pro Tyr Lys Gly Lys Pro Lys Leu             180 185 190 Gly Phe Ala Gln Met Glu Glu Ile Gly Arg Arg Thr Gly Val Pro Leu         195 200 205 Val Leu His Gly Gly Thr Gly Ile Pro Leu Ala Asp Ile Gln Lys Ala     210 215 220 Ile Ser Phe Gly Thr Ala Lys Ile Asn Val Asn Thr Glu Asn Gln Trp 225 230 235 240 Ala Phe Thr Arg Gly Ile Cys Arg Met Phe Arg Glu Asn Glu Gly Leu                 245 250 255 Tyr Asp Pro Arg Lys Tyr Leu Gly Ala Gly Arg Glu Glu Val Lys Gln             260 265 270 Thr Val Lys Gln Lys Ile Arg Glu Phe Gly Ser Ala Lys Lys Ala Thr         275 280 285 Glu Ala Leu Leu Pro Ser Val     290 295 <210> 43 <211> 287 <212> PRT <213> Geobacillus sp. <400> 43 Met Pro Leu Val Ser Met Lys Glu Met Leu Asn Glu Ala Leu Arg Gly   1 5 10 15 Lys Tyr Ala Val Gly Gln Phe Asn Ile Asn Asn Leu Glu Trp Thr Gln              20 25 30 Ala Ile Leu Ala Ala Ala Glu          35 40 45 Val Ser Glu Gly Ala Ala Arg Tyr Met Ser Gly Phe Lys Thr Val Val      50 55 60 Asn Met Val Lys Gly Leu Met Glu Asp Met Asn Ile Thr Val Pro Val  65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Lys Cys Lys Ala Ala                  85 90 95 Ile Asp Ala Gly Phe Thr Ser Val Met Ile Asp Ala Ser His His Pro             100 105 110 Phe Glu Glu Asn Val Arg Ile Thr Ser Gln Val Val Glu Tyr Ala His         115 120 125 Ala Arg Gly Val Ser Val Glu Ala Glu Leu Gly Ile Val Gly Gly Gln     130 135 140 Glu Asp Val Val Gly Glu Gly Ile Ile Tyr Ala Asp Pro Lys Glu 145 150 155 160 Cys Glu Glu Leu Val Lys Arg Thr Gly Val Asp Cys Leu Ala Pro Ala                 165 170 175 Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Lys Leu Gly Phe             180 185 190 Ala Glu Met Glu Gln Ile Arg Asp Leu Thr Gly Val Pro Leu Val Leu         195 200 205 His Gly Gly Thr Gly Ile Pro Thr Glu Gln Ile Gln Arg Ile Ile Ser     210 215 220 Leu Gly Thr Ser Lys Ile Asn Val Asn Thr Glu Asn Gln Met Ala Phe 225 230 235 240 Thr Lys Val Val Arg Glu Leu Leu Ala Lys Asp Pro Asn Val Tyr Asp                 245 250 255 Pro Arg Lys Ile Ile Gly Pro Gly Arg Asp Ala Ile Lys Ala Thr Val             260 265 270 Ile Gly Lys Met Arg Glu Phe Gly Ser Ser Gly Lys Ala Ala Arg         275 280 285 <210> 44 <211> 287 <212> PRT <213> Geobacillus stearothermophilus <400> 44 Met Ser Leu Val Ser Met Lys Glu Met Leu Asn Glu Ala Leu Arg Gly   1 5 10 15 Lys Tyr Ala Val Gly Gln Phe Asn Ile Asn Asn Leu Glu Trp Thr Gln              20 25 30 Ala Ile Leu Ala Ala Ala Glu          35 40 45 Val Ser Glu Gly Ala Ala Arg Tyr Met Gly Gly Phe Lys Thr Val Val      50 55 60 Asn Met Val Lys Gly Leu Met Glu Asp Met Asn Ile Thr Val Pro Val  65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Lys Cys Lys Ala Ala                  85 90 95 Ile Asp Ala Gly Phe Thr Ser Val Met Ile Asp Ala Ser His His Pro             100 105 110 Phe Glu Glu Asn Val Arg Ile Thr Ser Gln Val Val Glu Tyr Ala His         115 120 125 Ala Arg Gly Val Ser Val Glu Ala Glu Leu Gly Ile Val Gly Gly Gln     130 135 140 Glu Asp Val Val Gly Glu Gly Val Ile Tyr Ala Asp Pro Lys Glu 145 150 155 160 Cys Glu Glu Leu Val Lys Arg Thr Gly Ile Asp Cys Leu Ala Pro Ala                 165 170 175 Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Lys Leu Gly Phe             180 185 190 Ala Glu Met Glu Lys Ile Arg Asp Leu Thr Gly Ile Pro Leu Val Leu         195 200 205 His Gly Gly Thr Gly Ile Pro Thr Glu Gln Ile Gln Arg Ile Ile Ser     210 215 220 Leu Gly Thr Ser Lys Ile Asn Val Asn Thr Glu Asn Gln Ile Ala Phe 225 230 235 240 Thr Lys Ala Val Arg Glu Leu Leu Ala Lys Asp Pro Asn Val Tyr Asp                 245 250 255 Pro Arg Lys Ile Ile Gly Pro Gly Arg Asp Ala Ile Lys Ala Thr Val             260 265 270 Ile Gly Lys Met Arg Glu Phe Gly Ser Ser Gly Lys Ala Ala Gln         275 280 285 <210> 45 <211> 295 <212> PRT <213> Geobacillus thermodenitrificans <400> 45 Met Pro Leu Val Ser Met Lys Glu Met Leu Gln Cys Ala Leu Gln Gly   1 5 10 15 Asn Tyr Ala Val Gly His Phe Asn Val Asn Asn Leu Glu Phe Ala Gln              20 25 30 Ala Ile Leu Glu Ala Glu Glu Glu Glu Glu Ala Pro Val Ile Leu Ala          35 40 45 Val Ser Pro Gly Tyr Ile Gly His Leu Gly Gly Leu Arg Thr Ala Ala      50 55 60 Ala Met Val Lys Glu Leu Val Lys Glu Tyr Arg Ile Thr Val Pro Val  65 70 75 80 Ala Leu His Leu Asp His Gly Ser Ser Tyr Glu Gln Cys Leu Glu Ala                  85 90 95 Met Glu Ala Gly Phe Thr Ser Val Met Ile Asp Ala Ser His Phe Pro             100 105 110 Leu Glu Glu Asn Ile Ser Met Thr Lys Lys Val Val Glu Ala Ala Arg         115 120 125 Phe Phe Gly Val Ser Val Glu Ala Glu Val Gly Arg Ile Gly Gly Gln     130 135 140 Glu Asp Val Val Val Val Asp Glu Ala Glu Ala Met Tyr Ala Ile Pro 145 150 155 160 Glu Glu Cys Glu Arg Leu Val Lys Glu Thr Gly Val Asp Cys Leu Ala                 165 170 175 Pro Ala Leu Gly Ser Val His Gly Pro Tyr Lys Gly Lys Pro Lys Leu             180 185 190 Gly Phe Ala Gln Met Glu Gln Ile Gln Arg Leu Thr Gly Val Pro Leu         195 200 205 Val Leu His Gly Gly Thr Gly Ile Pro Leu Glu Asp Ile Arg Arg Ala     210 215 220 Ile Ser Leu Gly Thr Ala Lys Ile Asn Val Asn Thr Glu Asn Gln Leu 225 230 235 240 Ala Phe Thr Arg Gly Val Arg Ser Leu Leu Asn Glu Asn Asn Gly Leu                 245 250 255 Tyr Asp Pro Arg Lys Tyr Leu Gly Ala Gly Arg Glu Glu Val Lys Gln             260 265 270 Thr Val Arg Gln Lys Ile Arg Glu Phe Gly Ser Val Gly Lys Ala Lys         275 280 285 Glu Gly Val Leu Ser Seru     290 295 <210> 46 <211> 303 <212> PRT <213> Lactobacillus acidophilus <400> 46 Met Ala Tyr Phe Val Asn Gly Asn Glu Ile Phe Arg Glu Ala Arg Lys   1 5 10 15 His His Tyr Ala Val Gly Ala Tyr Asn Thr Asn Asn Leu Glu Trp Thr              20 25 30 Arg Ala Glu Leu Arg Ala Ala Glu Glu Thr Arg Thr Pro Leu Leu Ile          35 40 45 Gln Val Ser Thr Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Phe Val      50 55 60 Lys Asp Met Val Glu Asp Gln Met Asp Ser Met Asn Ile Ser Val Pro  65 70 75 80 Val Ile Leu Asn Leu Asp His Ser Asp Phe Glu Ser Ala Lys Glu Cys                  85 90 95 Ile Ala Leu Gly Tyr Ser Ser Val Met Phe Asp Gly His Ala Leu Pro             100 105 110 Thr Glu Glu Asn Leu Ala Lys Thr Lys Glu Ile Ile Lys Leu Ala His         115 120 125 Glu Arg Gly Ile Ser Val Glu Ala Glu Ile Gly Lys Ile Gly Glu Asn     130 135 140 Gln Gly Gly Gly Glu Leu Ala Ser Val Glu Asp Ala Lys Ala Phe Val 145 150 155 160 Glu Ala Gly Val Asp Lys Leu Ala Cys Gly Ile Gly Asn Ile His Gly                 165 170 175 Val Tyr Pro Ala Asp Trp Lys Gly Leu Asn Phe Asp Arg Leu Lys Glu             180 185 190 Ile Ala Glu Ala Val Pro Glu Thr Pro Leu Val Leu His Gly Gly Ser         195 200 205 Gly Ile Pro Glu Asp Gln Ile Lys Lys Ala Ile Gln Leu Gly Ile Ser     210 215 220 Lys Val Asn Ile Asn Thr Glu Phe Gln Leu Ala Phe Gln Glu Ala Thr 225 230 235 240 Arg Lys Tyr Ile Glu Glu Gly Lys Asp Glu Asp Lys Ala Asn Lys Gly                 245 250 255 Tyr Asp Pro Arg Lys Leu Leu Leu Pro Gly Thr Glu Ala Ile Thr Asp             260 265 270 Lys Met Lys Glu Met Ile Ala Trp Leu Gly Thr Lys Thr Ile Asp Asp         275 280 285 Glu Leu Lys Asp Ala Ser Phe Asp Arg Ser Ser Leu Asn Glu Glu     290 295 300 <210> 47 <211> 304 <212> PRT <213> Lactobacillus amylolyticus <400> 47 Met Ala Tyr Phe Asp Asn Gly Asn Gln Ile Phe Lys Asp Ala Arg Arg   1 5 10 15 Glu His Tyr Ala Val Gly Ala Tyr Asn Thr Asn Asn Leu Glu Trp Thr              20 25 30 Arg Ala Glu Leu Arg Ala Ala Glu Glu Thr Arg Thr Pro Leu Leu Ile          35 40 45 Gln Val Ser Thr Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Phe Val      50 55 60 Lys Asp Met Val Ala Asp Gln Ile Asp Ser Met Asn Ile Ser Val Pro  65 70 75 80 Val Ile Leu Asn Leu Asp His Gly Asp Tyr Glu Ser Ala Lys Glu Cys                  85 90 95 Ile Ala Leu Gly Tyr Ser Ser Val Met Phe Asp Gly His Ala Leu Pro             100 105 110 Val Glu Glu Asn Leu Ala Lys Thr Lys Glu Ile Ile Lys Leu Ala His         115 120 125 Glu Arg Gly Ile Ser Val Glu Ala Glu Ile Gly Lys Ile Gly Glu Asn     130 135 140 Gln Gly Ala Asp Gly Gly Glu Leu Ala Ser Val Glu Asp Ala Ile Thr 145 150 155 160 Phe Tyr Lys Ala Gly Val Asp Lys Leu Ala Cys Gly Ile Gly Asn Ile                 165 170 175 His Gly Val Tyr Pro Glu Gly Trp Lys Gly Leu Asn Phe Asp Arg Leu             180 185 190 Lys Glu Ile Ala Asp Ala Val Pro Val Leu Val Leu His Gly Gly         195 200 205 Ser Gly Ile Pro Glu Asp Gln Val Lys Lys Ala Ile Ser Leu Gly Ile     210 215 220 Ala Lys Val Asn Ile Asn Thr Glu Phe Gln Leu Ala Phe Gln Lys Ala 225 230 235 240 Thr Arg Lys Tyr Ile Glu Asp Lys Lys Asp Leu Asp Thr Ala Asn Lys                 245 250 255 Gly Tyr Asp Pro Arg Lys Leu Leu Leu Pro Gly Thr Glu Ala Ile Thr             260 265 270 Ala Ala Met Lys Glu Met Ile Ser Trp Leu Gly Thr Lys Ser Ile Asp         275 280 285 Asp Glu Leu Lys Asp Ala Ser Phe Asp Arg Ala Ser Leu Asn Glu Glu     290 295 300 <210> 48 <211> 295 <212> PRT <213> Lactobacillus antri <400> 48 Met Arg Lys Glu Val Asn Thr Met Ala Tyr Phe Asp Asn Gly Asn Gln   1 5 10 15 Ile Phe Ala Asp Ala Arg Ala Asn His Tyr Ala Val Gly Ala Tyr Asn              20 25 30 Ile Asn Asn Leu Glu Trp Thr Arg Ala Ile Leu Gln Ala Ala Asn Glu          35 40 45 Thr Arg Thr Pro Val Leu Val Gln Val Ser Met Gly Ala Ala Lys Tyr      50 55 60 Met Gly Gly Phe Lys Phe Val Arg Asp Met Val Ala Asp Gln Ile Asp  65 70 75 80 Ala Met Gly Ile Thr Val Val Val Leu Asn Leu Asp His Gly Asp                  85 90 95 Phe Ala Ala Ala Met Glu Cys Ile Asp Leu Gly Tyr Ser Ser Val Met             100 105 110 Tyr Asp Gly His Lys Leu Pro Leu Ala Thr Asn Tyr Asp Gln Thr Ala         115 120 125 Gln Ile Val Gln Ala Ala His Gln Arg Gly Ile Ser Val Glu Ala Glu     130 135 140 Ile Gly Lys Val Gly Glu Asn Gln Asp Thr Asn Ser Gly Glu Leu Ala 145 150 155 160 Ser Val Ala Asp Ala Lys Lys Phe Ala Glu Leu Gly Val Asp Lys Leu                 165 170 175 Ala Cys Gly Ile Gly Asn Ile His Gly Ile Tyr Pro Ala Asp Trp Arg             180 185 190 Gly Leu Asp Phe Asp Arg Leu Ala Ala Ile Ala Asp Ala Val Asp Ile         195 200 205 Pro Leu Val Leu His Gly Gly Ser Gly Ile Pro Lys Asp Gln Val Gln     210 215 220 Arg Ala Ile Ala Leu Gly Ile Ala Lys Val Asn Ile Asn Thr Glu Phe 225 230 235 240 Gln Leu Ala Phe Gln Ala Ala Thr Arg Asp Tyr Ile Glu Gln Ala Arg                 245 250 255 Asp Leu Asp Gln Ala Gln Lys Gly Tyr Asp Pro Arg Lys Leu Leu Leu             260 265 270 Pro Gly Thr Glu Ala Ile Thr Ala Lys Met Lys Glu Ile Ile Asp Trp         275 280 285 Leu Gly Thr Pro Ala Val Ala     290 295 <210> 49 <211> 293 <212> PRT <213> Lactobacillus casei <400> 49 Met Pro Leu Val Asn Ala Ala Glu Leu Val Lys Ala Ala His Lys Gly   1 5 10 15 His Tyr Cys Ile Gly Ala Phe Asn Thr Asn Asn Leu Glu Trp Thr Arg              20 25 30 Ala Ile Leu Ala Gly Ale Gln Glu Leu Asn Val          35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Glu Phe Cys Gln      50 55 60 Thr Met Ile Glu Ala Thr Val Lys Ala Met Gly Ile Asn Val Val  65 70 75 80 Val Ile His Leu Asp His Gly Asn Tyr Glu Ala Ala Lys Glu Ala Ile                  85 90 95 Ala Ala Gly Tyr Asn Ser Val Met Phe Asp Gly His Asp Leu Asp Phe             100 105 110 Glu Asp Asn Leu Glu Lys Thr Lys Glu Ile Val Lys Leu Ala His Ala         115 120 125 Lys Gly Ile Ser Val Glu Ala Glu Val Gly Ser Ile Gly Gly Glu Glu     130 135 140 Asp Gly Val Val Gly Glu Gly Glu Leu Ala Asp Val Glu Glu Ala Lys 145 150 155 160 Thr Leu Ala Ala Thr Gly Ile Asp Phe Leu Ala Ala Gly Ile Gly Asn                 165 170 175 Ile His Gly Gln Tyr Pro Asp Asn Trp Lys Gly Leu His Phe Asp Arg             180 185 190 Leu Gln Glu Leu Asn Asp Ala Val Lys Met Pro Leu Val Leu His Gly         195 200 205 Gly Ser Gly Ile Pro Gln Glu Gln Val Gln Lys Ala Ile Thr Met Gly     210 215 220 Ile Ser Lys Leu Asn Ile Asn Thr Glu Cys Gln Leu Ala Phe Ala Lys 225 230 235 240 Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Gln Gln Gly Lys Gly                 245 250 255 Phe Asp Pro Arg Lys Leu Leu Lys Pro Gly Thr Asp Ala Ile Thr Asp             260 265 270 Thr Phe Lys Glu Ile Thr Gly Trp Ile Gly Asn Lys Pro Val Lys Met         275 280 285 Val Pro Glu Ala Leu     290 <210> 50 <211> 303 <212> PRT <213> Lactobacillus crispatus <400> 50 Met Ala Tyr Phe Asp Asn Gly Asn Gln Ile Phe Lys Asp Ala Arg Lys   1 5 10 15 Asn His Tyr Ala Val Gly Ala Tyr Asn Thr Asn Asn Leu Glu Trp Thr              20 25 30 Arg Ala Glu Leu Arg Ala Ala Glu Glu Thr Arg Thr Pro Leu Leu Ile          35 40 45 Gln Val Ser Thr Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Phe Val      50 55 60 Lys Asp Met Val Ala Asp Gln Met Asp Ser Met Asn Ile Ser Val Pro  65 70 75 80 Val Ile Leu Asn Leu Asp His Gly Asp Phe Glu Ser Ala Lys Glu Cys                  85 90 95 Ile Ala Leu Gly Tyr Ser Ser Val Met Phe Asp Gly His Ala Leu Pro             100 105 110 Thr Asp Glu Asn Leu Glu Lys Thr Lys Glu Ile Ile Lys Leu Ala His         115 120 125 Glu Arg Gly Ile Ser Val Glu Ala Glu Ile Gly Lys Ile Gly Glu Asn     130 135 140 Gln Gly Gly Gly Glu Leu Ala Ser Val Glu Asp Ala Lys Lys Phe Val 145 150 155 160 Ala Ala Gly Val Asp Lys Leu Ala Cys Gly Ile Gly Asn Ile His Gly                 165 170 175 Val Tyr Pro Ala Asp Trp Lys Gly Leu Asn Phe Asp Arg Leu Lys Glu             180 185 190 Ile Ala Glu Ala Val Pro Asp Thr Pro Leu Val Leu His Gly Gly Ser         195 200 205 Gly Ile Pro Glu Asp Gln Val Lys Lys Ala Ile Lys Leu Gly Ile Ala     210 215 220 Lys Ile Asn Ile Asn Thr Glu Phe Gln Leu Ala Phe Gln Glu Ala Thr 225 230 235 240 Arg Lys Tyr Ile Glu Asp Glu Lys Asp Leu Asp Lys Lys Ala Lys Gly                 245 250 255 Tyr Asp Pro Arg Lys Leu Leu Leu Pro Gly Ala Glu Ala Ile Thr Ala             260 265 270 Lys Met Lys Glu Met Ile Ser Trp Met Gly Thr Lys Thr Ile Asp Asp         275 280 285 Glu Leu Lys Asp Ala Ser Phe Asp Arg Ser Ser Leu Asn Glu Glu     290 295 300 <210> 51 <211> 287 <212> PRT <213> Lactobacillus curvatus <400> 51 Met Ser Leu Val Asn Gly Thr Glu Ile Phe Lys Ala Ala Arg Glu Gly   1 5 10 15 His Tyr Ala Val Gly Ala Phe Asn Thr Asn Asn Leu Glu Trp Thr Arg              20 25 30 Ala Ile Leu Gly Ala Ala Gln Glu Thr Asn Thr Pro Ile Leu Ile Gln          35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Glu Leu Cys Lys      50 55 60 Asn Leu Val Glu Gln Thr Ile Lys Ser Met Asn Ile Thr Val Pro Val  65 70 75 80 Val Met His Leu Asp His Gly Asn Tyr Glu Ala Ala Lys Glu Cys Ile                  85 90 95 Glu Val Gly Tyr Asn Ser Val Met Phe Asp Gly His Asp Leu Pro Phe             100 105 110 Glu Glu Asn Leu Ala Lys Thr Lys Glu Ile Val Ala Leu Ala His Ala         115 120 125 Lys Gly Ile Ser Val Glu Ala Glu Val Gly Ser Ile Gly Gly Glu Glu     130 135 140 Asp Gly Ile Gly Aly Gly Aly Gly Leu Ala Asp Val Glu Glu Ala Lys 145 150 155 160 Gln Leu Ala Ala Aly Gly Pro Asp Tyr Leu Ala Val Gly Ile Gly Asn                 165 170 175 Ile His Gly Val Tyr Pro Glu Asn Trp Gln Gly Leu Ser Phe Asp Arg             180 185 190 Leu Gln Glu Leu Ala Asp Val Ile Asp Leu Pro Leu Val Leu His Gly         195 200 205 Gly Ser Gly Ile Pro Lys Glu Gln Ile Leu Lys Ala Ile Glu Met Gly     210 215 220 Ile Ser Lys Val Asn Val Asn Thr Glu Gln Gln Ile Ala Trp Ser Asn 225 230 235 240 Ala Val Lys Glu Tyr Tyr Ala Glu Asn Lys Asp Gln Ala Glu Lys Gly                 245 250 255 Phe Asp Pro Arg Lys Val Leu Ala Pro Gly Thr Lys Ala Ile Lys Asp             260 265 270 Thr Val Glu Ser Arg Ile Glu Trp Phe Gly Thr Pro Ala Val Lys         275 280 285 <210> 52 <211> 303 <212> PRT <213> Lactobacillus delbrueckii subsp. bulgaricus <400> 52 Met Ala Tyr Phe Val Asn Gly Asn Asp Ile Phe Lys Lys Ala Arg Lys   1 5 10 15 Glu His Tyr Ala Val Gly Ala Tyr Asn Thr Asn Asn Leu Glu Trp Thr              20 25 30 Arg Ala Leu Leu Arg Gly Ala Glu Glu Thr Arg Thr Pro Leu Leu Ile          35 40 45 Gln Val Ser Thr Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Leu Val      50 55 60 Lys Asp Leu Val Glu Asp Ser Met Asp Ala Met Asn Ile Ser Val Pro  65 70 75 80 Val Ile Leu Asn Leu Asp His Gly Asp Tyr Asp Ser Ala Ile Glu Cys                  85 90 95 Ile Lys Leu Gly Tyr Ser Val Met Phe Asp Gly His Ala Leu Pro             100 105 110 Thr Glu Glu Asn Leu Ala Lys Thr Lys Glu Ile Ile Lys Leu Ala His         115 120 125 Glu Arg Gly Ile Ser Val Glu Ala Glu Ile Gly Lys Ile Gly Glu Asn     130 135 140 Gln Gly Ala Gly Glu Leu Ala Ser Val Glu Asp Ala Lys Ala Phe Val 145 150 155 160 Ala Ala Gly Val Asp Lys Leu Ala Cys Gly Ile Gly Asn Ile His Gly                 165 170 175 Val Tyr Pro Glu Gly Trp Ser Gly Leu Asn Phe Asp Arg Leu Lys Glu             180 185 190 Ile Ala Glu Ala Val Pro Glu Thr Pro Leu Val Leu His Gly Gly Ser         195 200 205 Gly Ile Pro Gln Asp Gly Ile Glu Lys Ala Ile Ser Leu Gly Ile Ser     210 215 220 Lys Ile Asn Ile Asn Thr Glu Phe Gln Leu Ala Phe Gln Glu Ala Thr 225 230 235 240 Arg Lys Tyr Ile Glu Glu Gly Lys Asp Gln Asp Lys Ser Lys Lys Gly                 245 250 255 Tyr Asp Pro Arg Lys Leu Leu Leu Pro Gly Thr Glu Ala Ile Thr Asp             260 265 270 Ser Met Lys Glu Met Ile Ser Trp Met Gly Thr Pro Ser Ile Asp Asp         275 280 285 Lys Glu Ala Asp Ala Ser Phe Asp Arg Ser Ser Leu Asn Glu Glu     290 295 300 <210> 53 <211> 303 <212> PRT <213> Lactobacillus helveticus <400> 53 Met Ala Tyr Phe Asp Asn Gly Asn Glu Ile Phe Lys Glu Ala Arg Lys   1 5 10 15 His His Tyr Ala Val Gly Ala Tyr Asn Thr Asn Asn Leu Glu Trp Thr              20 25 30 Arg Ala Glu Leu Arg Ala Ala Glu Glu Thr Arg Thr Pro Leu Leu Ile          35 40 45 Gln Val Ser Thr Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Phe Val      50 55 60 Lys Asp Met Val Ala Asp Gln Met Asp Ser Met Asn Ile Ser Val Pro  65 70 75 80 Val Ile Leu Asn Leu Asp His Gly Asp Phe Glu Ser Ala Lys Glu Cys                  85 90 95 Ile Ala Leu Gly Tyr Ser Ser Val Met Phe Asp Gly His Ala Leu Pro             100 105 110 Thr Glu Glu Asn Leu Ala Lys Thr Lys Glu Ile Ile Lys Leu Ala His         115 120 125 Glu Arg Gly Ile Ser Val Glu Ala Glu Ile Gly Lys Ile Gly Glu Asn     130 135 140 Gln Gly Gly Gly Glu Leu Ala Ser Val Glu Asp Ala Lys Lys Phe Val 145 150 155 160 Ala Ala Gly Val Asp Lys Leu Ala Cys Gly Ile Gly Asn Ile His Gly                 165 170 175 Val Tyr Pro Ala Asp Trp Lys Gly Leu Asn Phe Asp Arg Leu Lys Glu             180 185 190 Ile Ala Glu Ala Val Pro Asp Thr Pro Leu Val Leu His Gly Gly Ser         195 200 205 Gly Ile Pro Glu Asp Gln Val Lys Lys Ala Ile Lys Leu Gly Ile Ala     210 215 220 Lys Ile Asn Ile Asn Thr Glu Phe Gln Leu Ala Phe Gln Asp Ala Thr 225 230 235 240 Arg Lys Tyr Ile Glu Ala Glu Lys Asp Leu Asp Lys Lys Asn Lys Gly                 245 250 255 Tyr Asp Pro Arg Lys Leu Leu Leu Pro Gly Ala Glu Ala Ile Thr Ala             260 265 270 Lys Met Lys Glu Met Ile Ser Trp Met Gly Thr Lys Thr Ile Asp Asp         275 280 285 Glu Leu Lys Asp Val Ser Phe Asp Arg Ser Ser Leu Asn Glu Glu     290 295 300 <210> 54 <211> 304 <212> PRT <213> Lactobacillus iners <400> 54 Met Ala Tyr Leu Val Asn Gly Asn Asp Ile Phe Lys Ala Ala Arg Lys   1 5 10 15 Asn His Tyr Ala Val Gly Ala Tyr Asn Thr Asn Asn Leu Glu Trp Thr              20 25 30 Arg Ala Leu Ile Lys Gly Ala Lys Glu Thr Arg Thr Pro Leu Leu Ile          35 40 45 Gln Val Ser Thr Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Thr Val      50 55 60 Arg Asp Leu Val Leu Asn Glu Met Asp Asn Met Asp Val Asp Val Pro  65 70 75 80 Val Ile Leu Asn Leu Asp His Gly Asp Tyr Glu Ser Ala Lys Glu Cys                  85 90 95 Ile Ala Leu Gly Tyr Ser Ser Val Met Phe Asp Gly His Lys Leu Pro             100 105 110 Val Glu Glu Asn Leu Glu Lys Thr Lys Glu Ile Val Lys Leu Ala His         115 120 125 Glu Arg Gly Ile Ser Val Glu Ala Glu Ile Gly Lys Ile Gly Glu Asn     130 135 140 Gln Gly Ala Asp Gly Gly Glu Leu Ala Ser Val Glu Asp Ala Lys Arg 145 150 155 160 Phe Val Ala Ala Gly Val Asp Lys Leu Ala Cys Gly Ile Gly Asn Ile                 165 170 175 His Gly Val Tyr Pro Ala Asp Trp Lys Gly Leu Asn Phe Asp Arg Leu             180 185 190 Lys Glu Ile Ala Asp Ala Val Asp Val Pro Leu Val Leu His Gly Gly         195 200 205 Ser Gly Ile Pro Gln Asp Gly Ile Lys Lys Ser Ale Ser Leu Gly Ile     210 215 220 Ala Lys Ile Asn Ile Asn Thr Glu Phe Gln Leu Ala Phe Gln Glu Ala 225 230 235 240 Thr Arg Lys Tyr Ile Glu Ala Glu Lys Asp Leu Asp Lys Ala Asn Lys                 245 250 255 Gly Tyr Asp Pro Arg Lys Leu Leu Arg Ala Gly Thr Asp Ala Ile Thr             260 265 270 Glu Ser Met Lys Glu Met Ile Ser Trp Met Gly Thr Arg Pro Ile Asp         275 280 285 Gly Lys Asp Ser Val Val Lys Phe Asp Glu Ala Ser Leu Asn Glu Glu     290 295 300 <210> 55 <211> 305 <212> PRT <213> Lactobacillus johnsonii <400> 55 Met Ala Tyr Leu Val Asn Gly Asn Asp Ile Phe Lys Ala Ala Arg Glu   1 5 10 15 Asn His Tyr Ala Val Gly Ala Tyr Asn Thr Asn Asn Leu Glu Trp Thr              20 25 30 Arg Ala Leu Leu Arg Gly Ala Lys Glu Thr Arg Thr Pro Leu Leu Ile          35 40 45 Gln Val Ser Thr Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Thr Val      50 55 60 Lys Asp Leu Val Leu Asn Glu Met Asp Asn Met Asp Ile Asp Val Pro  65 70 75 80 Val Ile Leu Asn Leu Asp His Gly Asp Phe Glu Ser Ala Lys Glu Cys                  85 90 95 Ile Ala Leu Gly Tyr Ser Ser Val Met Phe Asp Gly His Asn Leu Pro             100 105 110 Thr Asp Glu Asn Leu Ala Lys Thr Lys Glu Ile Val Lys Leu Ala His         115 120 125 Glu Arg Gly Ile Ser Val Glu Ala Glu Ile Gly Lys Ile Gly Glu Asn     130 135 140 Gln Gly Ala Asp Gly Gly Glu Leu Ala Ser Val Glu Asp Ala Lys Thr 145 150 155 160 Phe Val Ala Ala Gly Val Asp Lys Leu Ala Cys Gly Ile Gly Asn Ile                 165 170 175 His Gly Val Tyr Pro Glu Gly Trp Thr Gly Leu Asn Phe Asp Arg Leu             180 185 190 Lys Glu Ile Ala Glu Ala Val Pro Asn Glu Pro Leu Val Leu His Gly         195 200 205 Gly Ser Gly Ile Pro Gln Asp Gln Ile Glu Lys Ala Ile Gln Leu Gly     210 215 220 Ile Ala Lys Ile Asn Ile Asn Thr Glu Phe Gln 225 230 235 240 Ala Thr Arg Lys Tyr Ile Glu Asp Lys Met Asp Leu Asp Lys Gly Asn                 245 250 255 Lys Gly Tyr Asp Pro Arg Lys Leu Leu Arg Ala Gly Thr Asp Ala Ile             260 265 270 Thr Asp Ser Met Lys Glu Met Ile Ser Trp Met Gly Thr Ala Pro Ile         275 280 285 Asp Thr Lys Glu Ser Ser Val Gln Phe Asp Glu Ala Ser Leu Asn Glu     290 295 300 Glu 305 <210> 56 <211> 288 <212> PRT <213> Lactobacillus murinus <400> 56 Met Ser Phe Val Asn Gly Ser Glu Ile Phe Ser Ala Ala Arg Lys Gly   1 5 10 15 His Tyr Ala Val Gly Ala Phe Asn Thr Asn Asn Leu Glu Trp Thr Arg              20 25 30 Ala Ile Leu Lys Gly Ala Gln Glu Lys Asn Val Pro Val Leu Ile Gln          35 40 45 Val Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Leu Val Arg      50 55 60 Asp Leu Val Ala Ser Glu Met Glu Ala Met Asn Ile Thr Val Pro Val  65 70 75 80 Val Met His Leu Asp His Gly Asn Tyr Glu Ala Ala Lys Glu Cys Ile                  85 90 95 Glu Ala Gly Phe Ser Ser Val Met Phe Asp Gly His Asp Leu Pro Phe             100 105 110 Glu Glu Asn Leu Ala Lys Thr Lys Glu Ile Val Glu Leu Ala His Ala         115 120 125 Lys Gly Ile Ser Val Glu Ala Glu Val Gly Ser Ile Gly Gly Glu Glu     130 135 140 Asp Gly Val Val Gly Ala Gly Glu Leu Ala Asp Val Glu Glu Ala Lys 145 150 155 160 Gln Ile Ala Ala Leu Gly Val Asp Tyr Leu Ala Cys Gly Ile Gly Asn                 165 170 175 Ile His Gly Gln Tyr Pro Ala Asn Trp Gln Gly Leu Asn Phe Asp Arg             180 185 190 Leu Lys Glu Leu Ala Asp Ala Met Pro Asp Met Pro Leu Val Leu His         195 200 205 Gly Gly Ser Gly Ile Pro Gln Glu Gln Val Val Lys Ala Ile Ser Met     210 215 220 Gly Val Ser Lys Leu Asn Ile Asn Thr Glu Cys Gln Ile Ala Phe Ala 225 230 235 240 Glu Ala Thr Arg Lys Tyr Ile Glu Glu Gly Lys Asp Lys Glu Gly Lys                 245 250 255 Gly Tyr Asp Pro Arg Lys Leu Leu Ala Pro Gly Thr Gln Ala Ile Thr             260 265 270 Asp Thr Val Glu Glu Ile Ile Asp Trp Ile Gly Thr Pro Glu Ala Lys         275 280 285 <210> 57 <211> 293 <212> PRT <213> Lactobacillus paracasei subsp. paracasei <400> 57 Met Pro Leu Val Asn Ala Ala Glu Leu Val Lys Ala Ala His Lys Gly   1 5 10 15 His Tyr Cys Ile Gly Ala Phe Asn Thr Asn Asn Leu Glu Trp Thr Arg              20 25 30 Ala Ile Leu Ala Gly Ale Gln Glu Leu Asn Val          35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Glu Phe Cys Gln      50 55 60 Thr Met Ile Glu Ala Thr Val Lys  65 70 75 80 Val Ile His Leu Asp His Gly Asn Tyr Glu Ala Ala Lys Glu Ala Ile                  85 90 95 Ala Ala Gly Tyr Asn Ser Val Met Phe Asp Gly His Asp Leu Asp Phe             100 105 110 Glu Asp Asn Leu Glu Lys Thr Lys Glu Ile Val Lys Leu Ala His Ala         115 120 125 Lys Gly Ile Ser Val Glu Ala Glu Val Gly Ser Ile Gly Gly Glu Glu     130 135 140 Asp Gly Val Val Gly Glu Gly Glu Leu Ala Asp Val Glu Glu Ala Lys 145 150 155 160 Thr Leu Ala Ala Thr Gly Ile Asp Phe Leu Ala Ala Gly Ile Gly Asn                 165 170 175 Ile His Gly Gln Tyr Pro Asp Asn Trp Lys Gly Leu His Phe Asp Arg             180 185 190 Leu Gln Glu Leu Asn Asp Ala Val Lys Met Pro Leu Val Leu His Gly         195 200 205 Gly Ser Gly Ile Pro Gln Glu Gln Val Gln Lys Ala Ile Thr Met Gly     210 215 220 Ile Ser Lys Leu Asn Ile Asn Thr Glu Cys Gln Leu Ala Phe Ala Lys 225 230 235 240 Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Gln Gln Gly Lys Gly                 245 250 255 Phe Asp Pro Arg Lys Leu Leu Lys Pro Gly Thr Asp Ala Ile Thr Asp             260 265 270 Thr Phe Lys Glu Ile Thr Gly Trp Ile Gly Asn Lys Pro Val Lys Met         275 280 285 Val Pro Glu Ala Leu     290 <210> 58 <211> 287 <212> PRT <213> Lactobacillus plantarum subsp. plantarum <400> 58 Met Ser Phe Val Asn Ala Val Asp Met Thr Gln Asp Ala Tyr Lys Asn   1 5 10 15 His Tyr Ala Ile Gly Ala Phe Asn Thr Asn Asn Leu Glu Trp Thr Arg              20 25 30 Cys Ile Leu Lys Gly Ala Gln Asp Thr Asn Thr Pro Val Ile Ile Gln          35 40 45 Val Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Leu Val Met      50 55 60 Asp Leu Val Ala Asp Glu Met Glu Ser Met Asn Ile Thr Val Pro Val  65 70 75 80 Val Met His Leu Asp His Gly Asn Tyr Glu Ala Ala Lys Glu Cys Ile                  85 90 95 Glu Val Gly Tyr Ser Ser Val Met Phe Asp Gly His Asp Leu Pro Phe             100 105 110 Ala Glu Asn Leu Glu Lys Thr Lys Glu Ile Val Lys Leu Ala His Ala         115 120 125 Lys Gly Ile Ser Val Glu Ala Glu Val Gly Ser Ile Gly Gly Glu Glu     130 135 140 Asp Gly Ile Gly Gly Glu Glu Leu Ala Asp Val Glu Glu Ala Lys 145 150 155 160 Thr Leu Ala Ala Thr Gly Ile Asp Ile Leu Ala Ala Gly Ile Gly Asn                 165 170 175 Ile His Gly Lys Tyr Pro Glu Asn Trp Thr Gly Leu His Phe Asp Arg             180 185 190 Leu Gln Glu Ile Ser Glu Ala Val Lys Met Pro Leu Val Leu His Gly         195 200 205 Gly Ser Gly Ile Pro Lys Asp Gln Ile Val Lys Ala Val Glu Leu Gly     210 215 220 Ile Ser Lys Val Asn Val Asn Thr Glu Asn Gln Val Ala Phe Ala Asn 225 230 235 240 Ala Thr Arg Ala Tyr Ile Glu Ser Gly Ala Asp Gln Ile Gly Lys Gly                 245 250 255 Tyr Asp Pro Arg Lys Leu Leu Ala Pro Gly Lys Lys Ala Ile Val Asp             260 265 270 Val Val Thr Ser Arg Ile Gly Trp Phe Lys Thr Pro Ala Val Lys         275 280 285 <210> 59 <211> 289 <212> PRT <213> Lactobacillus reuteri <400> 59 Met Met Ala Tyr Phe Asp Asn Gly Asn Lys Leu Phe Asp Asp Ala Arg   1 5 10 15 Lys Asn Gln Tyr Ala Ile Gly Ala Tyr Asn Ile Asn Asn Leu Glu Trp              20 25 30 Thr Arg Ala Ile Leu Arg Ala Ala Ala Glu Thr Asn Thr Pro Val Leu          35 40 45 Ile Gln Val Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Phe      50 55 60 Val Lys Asp Met Val Glu Asp Gln Met Asp Ala Met Glu Ile Lys Val  65 70 75 80 Pro Val Ile Leu Asn Leu Asp His Gly Asp Tyr Asp Ala Ala Ile Glu                  85 90 95 Cys Ile Asn Leu Gly Tyr Ser Ser Val Met Phe Asp Gly His Lys Leu             100 105 110 Pro Ile Glu Glu Asn Tyr Ala Lys Thr Lys Glu Ile Val Gln Leu Ala         115 120 125 His Ser Arg Gly Ile Ala Val Glu Ala Glu Ile Gly Lys Val Gly Glu     130 135 140 Asn Gln Asp Thr Ser Ser Gly Glu Leu Ala Asp Val Glu Asp Ala Met 145 150 155 160 Ala Phe Ala Ala Met Gly Val Asp Arg Leu Ala Cys Gly Ile Gly Asn                 165 170 175 Ile His Gly Ile Tyr Pro Ala Asn Trp Pro Gly Leu Asp Phe Asp Arg             180 185 190 Leu Gln Glu Ile Ala Asn Ale Ile Asp Ile Pro Leu Val Leu His Gly         195 200 205 Gly Ser Gly Ile Pro Arg Glu Gln Val Gln Lys Ala Ile Ser Met Gly     210 215 220 Ile Ala Lys Val Asn Ile Asn Thr Glu Phe Gln Leu Ala Phe Gln Asp 225 230 235 240 Ala Thr Arg Lys Tyr Ile Glu Asn Glu Leu Asp Leu Asn Lys Glu Gln                 245 250 255 Lys Gly Tyr Asp Pro Arg Lys Leu Leu Leu Pro Gly Thr Asp Ala Ile             260 265 270 Thr Ala Lys Met Lys Asp Met Ile Glu Trp Leu Gly Thr Pro Ala Val         275 280 285 Ala     <210> 60 <211> 293 <212> PRT <213> Lactobacillus rhamnosus <400> 60 Met Pro Leu Val Asn Ala Ala Glu Leu Val Lys Ala Ala His Lys Gly   1 5 10 15 His Tyr Cys Ile Gly Ala Phe Asn Thr Asn Asn Leu Glu Trp Thr Arg              20 25 30 Ala Ile Leu Ala Gly Ale Gln Glu Leu Asn Val          35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Glu Phe Cys Gln      50 55 60 Thr Met Ile Gla Ala Ala Val Lys Ala Met Asp Ile Thr Val Val  65 70 75 80 Val Ile His Leu Asp His Gly Asn Tyr Glu Ala Ala Lys Glu Ala Ile                  85 90 95 Ala Ala Gly Tyr Asn Ser Val Met Phe Asp Gly His Asp Leu Asp Phe             100 105 110 Glu Asp Asn Leu Glu Lys Thr Lys Glu Ile Val Lys Leu Ala His Ala         115 120 125 Lys Gly Ile Ser Val Glu Ala Glu Val Gly Ser Ile Gly Gly Glu Glu     130 135 140 Asp Gly Val Val Gly Glu Gly Glu Leu Ala Asp Val Glu Glu Ala Lys 145 150 155 160 Thr Leu Ala Ala Thr Gly Ile Asp Phe Leu Ala Ala Gly Ile Gly Asn                 165 170 175 Ile His Gly Gln Tyr Pro Asp Asn Trp Lys Gly Leu His Phe Asp Arg             180 185 190 Leu Gln Glu Leu Asn Asp Ala Val Lys Met Pro Leu Val Leu His Gly         195 200 205 Gly Ser Gly Ile Pro Gln Glu Gln Val Gln Lys Ala Ile Thr Met Gly     210 215 220 Ile Ser Lys Leu Asn Ile Asn Thr Glu Cys Gln Leu Ala Phe Ala Lys 225 230 235 240 Ala Thr Arg Glu Tyr Ile Glu Ala Gly Lys Asp Gln Gln Gly Lys Gly                 245 250 255 Phe Asp Pro Arg Lys Met Leu Lys Pro Gly Thr Asp Ile Thr Asp             260 265 270 Thr Phe Lys Glu Ile Thr Gly Trp Ile Gly Asn Lys Pro Val Lys Met         275 280 285 Val Pro Glu Ala Leu     290 <210> 61 <211> 288 <212> PRT <213> Lactobacillus ruminis <400> 61 Met Ser Phe Val Asn Gly Asn Ala Ile Phe Gly Ala Ala Arg Lys Gly   1 5 10 15 His Tyr Cys Val Gly Ala Phe Asn Thr Asn Asn Leu Glu Trp Thr Arg              20 25 30 Ala Ile Leu Lys Gly Ala Gln Glu Lys Asn Val Pro Val Leu Ile Gln          35 40 45 Val Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Leu Val Arg      50 55 60 Asp Leu Val Ala Ser Glu Met Glu Ala Met Asn Ile Thr Val Pro Val  65 70 75 80 Val Met His Leu Asp His Gly Asn Tyr Glu Ala Ala Lys Glu Cys Ile                  85 90 95 Glu Ala Gly Tyr Ser Ser Val Met Phe Asp Gly His Asp Leu Pro Leu             100 105 110 Glu Glu Asn Leu Ala Lys Thr Lys Glu Ile Val Glu Leu Ala His Ala         115 120 125 His Gly Met Ser Val Glu Ala Glu Val Gly Ser Ile Gly Gly Thr Glu     130 135 140 Asp Gly Ile Thr Gly Ala Gly Asp Ala Thr 145 150 155 160 Lys Met Ala Ala Thr Gly Val Asp Tyr Leu Ala Cys Gly Ile Gly Asn                 165 170 175 Ile His Gly Lys Tyr Pro Ala Asp Trp Ala Gly Leu Asn Phe Asp Arg             180 185 190 Leu Lys Glu Ile Ala Asp Ala Leu Pro Asp Met Pro Leu Val Leu His         195 200 205 Gly Gly Ser Gly Ile Pro Glu Glu Gln Val Glu Lys Ala Ile Ser Leu     210 215 220 Gly Ile Ser Lys Leu Asn Ile Asn Thr Glu Cys Gln Leu Ala Phe Ala 225 230 235 240 Asp Ala Thr Arg Lys Tyr Ile Glu Ala Gly Lys Asp Gln Glu Gly Lys                 245 250 255 Gly Tyr Asp Pro Arg Lys Leu Leu Ala Pro Gly Thr Gln Ala Ile Thr             260 265 270 Asp Thr Val Glu Glu Ile Ile Gly Trp Met Gly Thr Pro Ser Ile Asp         275 280 285 <210> 62 <211> 288 <212> PRT <213> Lactobacillus salivarius <400> 62 Met Ser Val Phe Val Asn Gly Asn Ala Ile Phe Asn Ala Ala Arg Gln   1 5 10 15 Gly His Tyr Ala Val Gly Ala Phe Asn Thr Asn Asn Leu Glu Trp Thr              20 25 30 Arg Ala Ile Leu Lys Ala Ala Gln Glu Thr Asn Thr Pro Val Leu Ile          35 40 45 Gln Val Ser Met Gly Ala Ala Lys Tyr Met Gly Asp Tyr Lys Leu Val      50 55 60 Lys Lys Leu Val Glu Glu Glu Ile Arg Val Met Asn Ile Thr Val Pro  65 70 75 80 Val Ile Met His Leu Asp His Gly Asn Tyr Glu Ala Ala Lys Glu Cys                  85 90 95 Ile Glu Ala Gly Tyr Ser Ser Val Met Phe Asp Gly His Asp Leu Pro             100 105 110 Phe Glu Glu Asn Leu Glu Lys Thr Ala Glu Ile Val Lys Leu Ala His         115 120 125 Ala Lys Gly Ile Ser Val Glu Ala Glu Val Gly Ser Ile Gly Gly Glu     130 135 140 Glu Asp Gly Ile Val Gly Glu Gly Glu Leu Ala Ser Val Glu Glu Ala 145 150 155 160 Lys Gln Ile Ala Leu Gly Val Asp Tyr Leu Ala Cys Gly Ile Gly                 165 170 175 Asn Ile His Gly Pro Tyr Pro Glu Asn Trp Lys Gly Leu His Phe Asp             180 185 190 Arg Leu Gln Glu Ile Ala Asp Ala Ile Asp Thr Pro Leu Val Leu His         195 200 205 Gly Gly Ser Gly Ile Pro Gln Glu Gln Ile Glu Lys Ala Ile Lys Met     210 215 220 Gly Ile Ser Lys Val Asn Ile Asn Thr Glu Asn Gln Leu Ala Phe Ala 225 230 235 240 Ala Ala Thr Arg Lys Tyr Ile Glu Ala Gly Lys Asp Gln Glu Gly Lys                 245 250 255 Gly Tyr Asp Pro Arg Lys Leu Leu Ala Pro Gly Ser Glu Ala Ile Val             260 265 270 Asp Thr Val Lys Glu Ile Ile Gly Trp Leu Gly Thr Pro Glu Val Lys         275 280 285 <210> 63 <211> 303 <212> PRT <213> Lactobacillus sp. <400> 63 Met Ala Tyr Phe Val Asn Gly Asn Glu Ile Phe Arg Glu Ala Arg Lys   1 5 10 15 His His Tyr Ala Val Gly Ala Tyr Asn Thr Asn Asn Leu Glu Trp Thr              20 25 30 Arg Ala Glu Leu Arg Ala Ala Glu Glu Thr Arg Thr Pro Leu Leu Ile          35 40 45 Gln Val Ser Thr Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Phe Val      50 55 60 Lys Asp Met Val Glu Asp Gln Met Asp Ala Met Asn Ile Ser Val Pro  65 70 75 80 Val Ile Leu Asn Leu Asp His Gly Asp Phe Glu Ser Ala Lys Glu Cys                  85 90 95 Ile Ala Leu Gly Tyr Ser Ser Val Met Phe Asp Gly His Ala Leu Pro             100 105 110 Thr Glu Glu Asn Leu Ala Lys Thr Lys Glu Ile Ile Lys Leu Ala His         115 120 125 Glu Arg Gly Ile Ser Val Glu Ala Glu Ile Gly Lys Ile Gly Glu Asn     130 135 140 Gln Gly Gly Gly Glu Leu Ala Ser Val Glu Asp Ala Lys Ala Phe Val 145 150 155 160 Glu Ala Gly Val Asp Lys Leu Ala Cys Gly Ile Gly Asn Ile His Gly                 165 170 175 Val Tyr Pro Ala Asp Trp Lys Gly Leu Asn Phe Asp Arg Leu Lys Glu             180 185 190 Ile Ala Glu Ala Val Pro Glu Thr Pro Leu Val Leu His Gly Gly Ser         195 200 205 Gly Ile Pro Glu Asp Gln Ile Lys Lys Ala Ile Gln Leu Gly Ile Ser     210 215 220 Lys Val Asn Ile Asn Thr Glu Phe Gln Leu Ala Phe Gln Glu Ala Thr 225 230 235 240 Arg Lys Tyr Ile Glu Glu Gly Lys Asp Glu Asp Lys Ala Asn Lys Gly                 245 250 255 Tyr Asp Pro Arg Lys Leu Leu Leu Pro Gly Thr Glu Ala Ile Thr Asp             260 265 270 Lys Met Lys Glu Met Ile Ala Trp Leu Gly Thr Lys Thr Ile Asp Glu         275 280 285 Glu Ile Lys Asp Ala Ser Phe Asp Arg Ser Ser Leu Asn Glu Glu     290 295 300 <210> 64 <211> 354 <212> PRT <213> Marinobacter hydrocarbonoclasticus <400> 64 Met Ala Leu Ile Ser Leu Arg Gln Leu Leu Asp His Ala Ala Glu His   1 5 10 15 Gly Tyr Gly Val Ala Phe Asn Val Asn Asn Leu Glu Gln Met Arg              20 25 30 Ala Ile Met Glu Ala Ala Asp Lys Thr Asp Ser Pro Val Ile Val Gln          35 40 45 Ala Ser Ala Gly Ala Arg Lys Tyr Ala Gly Ala Pro Phe Leu Arg His      50 55 60 Leu Ile Leu Ala Ala Ile Glu Glu Trp Pro His Ile Pro Val Val Met  65 70 75 80 His Gln Asp His Gly Thr Ser Pro Ala Val Cys Gln Arg Ser Ile Gln                  85 90 95 Leu Gly Phe Ser Ser Val Met Met Asp Gly Ser Leu Gly Glu Asp Gly             100 105 110 Lys Thr Pro Thr Ser Tyr Glu Tyr Asn Val Asp Val Thr Arg Arg Thr         115 120 125 Val Glu Met Ala His Ala Cys Gly Val Ser Val Glu Gly Glu Leu Gly     130 135 140 Cys Leu Gly Ser Leu Glu Thr Gly Gln Ala Gly Glu Glu Asp Gly Ile 145 150 155 160 Gly Ala Glu Gly Thr Leu Asp His Ser Gln Met Leu Thr Asp Pro Glu                 165 170 175 Glu Ala Ala Asp Phe Val Gln Lys Thr Gln Val Asp Ala Leu Ala Ile             180 185 190 Ala Ile Gly Thr Ser His Gly Ala Tyr Lys Phe Thr Arg Pro Pro Thr         195 200 205 Gly Asp Ile Leu Ala Ile Asp Gln Ile Lys Ala Ile His Ala Arg Ile     210 215 220 Pro Asp Thr His Leu Val Met His Gly Ser Ser Ser Val Pro Gln Glu 225 230 235 240 Trp Leu Lys Val Ile Asn Glu Phe Gly Gly Glu Ile Pro Glu Thr Tyr                 245 250 255 Gly Val Pro Val Glu Gly Ile Val Glu Gly Ile Lys His Gly Val Arg             260 265 270 Lys Val Asn Ile Asp Thr Asp Leu Arg Leu Ala Ser Thr Gly Ala Val         275 280 285 Arg Arg Phe Met Ala Gln Asn Pro Ala Glu Phe Asp Pro Arg Lys Phe     290 295 300 Leu Lys Ala Thr Met Val Ala Met Thr Asp Ile Cys Val Ala Arg Tyr 305 310 315 320 Glu Ala Phe Gly Cys Ala Gly Gln Ala Ser Lys Ile Lys Pro Leu Asn                 325 330 335 Leu Glu Gln Met Phe Glu Arg Tyr Ala Ala Gly Glu Leu Asp Pro Lys             340 345 350 Val Lys         <210> 65 <211> 349 <212> PRT <213> Methylobacillus flagellatus <400> 65 Met Ala Leu Ile Ser Leu Arg Gln Leu Leu Asp His Ala Ala Glu His   1 5 10 15 Asp Tyr Gly Val Pro Ala Phe Asn Val Asn Asn Leu Glu Gln Val Gln              20 25 30 Ala Ile Met Arg Ala Ala Asp Ser Cys His Ser Pro Val Ile Leu Gln          35 40 45 Ala Ser Ala Gly Ala Arg Lys Tyr Ala Gly Glu Ala Phe Leu Leu Lys      50 55 60 Leu Ala Glu Ala Ala Ile Ala Ser Tyr Pro  65 70 75 80 His Gln Asp His Gly Ala Ser Pro Ala Ile Cys Leu His Ala Ile Gln                  85 90 95 Ser Gly Phe Ser Ser Val Met Met Asp Gly Ser Leu Leu Glu Asp Gly             100 105 110 Lys Thr Pro Ala Ser Tyr Glu Tyr Asn Val Glu Val Thr Arg Gln Val         115 120 125 Val Met Ala Ala His Ala Val Gly Val Ser Val Glu Gly Glu Leu Gly     130 135 140 Val Leu Gly Ser Leu Glu Thr Gly Leu Ala Gly Glu Glu Asp Gly Val 145 150 155 160 Gly Ala Glu Gly Arg Leu Asp Arg Ser Gln Leu Leu Thr Ser Pro Glu                 165 170 175 Glu Ala Val Asp Phe Val Ala Arg Thr Gly Val Asp Ala Leu Ala Val             180 185 190 Ala Ile Gly Thr Ser His Gly Ala Tyr Lys Phe Lys Arg Pro Pro Thr         195 200 205 Gly Glu Thr Leu Ala Ile Ser Arg Leu Arg Glu Ile His Ala Arg Leu     210 215 220 Pro Asn Thr His Leu Val Met His Gly Ser Ser Ser Val Pro Gln Glu 225 230 235 240 Trp Leu Gln Val Ile Asn Glu Tyr Gly Gly Glu Ile Pro Glu Thr Tyr                 245 250 255 Gly Val Pro Val Glu Glu Ile Val Lys Gly Ile Arg Ser Gly Val Arg             260 265 270 Lys Val Asn Ile Asp Thr Asp Leu Arg Leu Ala Phe Thr Gly Ala Leu         275 280 285 Arg Arg Phe Leu Gly Gln Pro Glu His Thr Ala Glu Phe Asp Pro Arg     290 295 300 Lys Ile Leu Ala Ala Thr Ser Ala Met Gln Thr Leu Cys Arg Glu 305 310 315 320 Arg Phe Glu Ala Phe Gly Ser Ala Gly Arg Ala Thr Gln Ile Gln Pro                 325 330 335 Val Pro Leu Asp Glu Met Ala Gln Arg Tyr Val Val Ser             340 345 <210> 66 <211> 354 <212> PRT <213> Rhizobium leguminosarum bv. trifoli <400> 66 Met Ala Arg Ile Thr Leu Arg Gln Leu Leu Asp His Ala Ala Glu Glu   1 5 10 15 Gly Tyr Gly Val Ala Phe Asn Ile Asn Asn Met Glu Gln Ala Leu              20 25 30 Ala Ile Met Glu Ala Ala Asp Ala Cys His Ala Pro Val Ile Met Gln          35 40 45 Ala Ser Arg Gly Ala Arg Ala Tyr Ala His Asp Ile Met Leu Lys His      50 55 60 Met Met Asp Ala Val Val Glu Ile Tyr Pro His Ile Pro Val Cys Val  65 70 75 80 His Leu Asp His Gly Asn Asp Pro Ser Asn Cys Met Thr Ala Ile Gln                  85 90 95 Ala Gly Phe Thr Ser Val Met Met Asp Gly Ser Leu Lys Ala Asp Ala             100 105 110 Lys Thr Pro Ala Asp Trp Ala Tyr Asn Val Gly Val Thr Lys Met Val         115 120 125 Thr Asp Met Ala His Phe Gly Gly Ile Ser Val Glu Gly Glu Leu Gly     130 135 140 Val Leu Gly Ser Leu Glu Thr Gly Met Gly Glu Ala Glu Asp Gly His 145 150 155 160 Gly Ala Glu Gly Lys Leu Ser His Asp Gln Leu Leu Thr Asp Pro Asp                 165 170 175 Glu Ala Val Lys Phe Val Arg Glu Thr Lys Val Asp Ala Leu Ala Ile             180 185 190 Ala Met Gly Thr Ser His Gly Ala Tyr Lys Phe Thr Arg Lys Pro Asp         195 200 205 Gly Ser Val Leu Ala Met Asn Val Ile Glu Glu Ile His Arg Lys Leu     210 215 220 Pro Asn Thr His Leu Val Met His Gly Ser Ser Ser Val Pro Ile Glu 225 230 235 240 Leu Gln Glu Ile Ile Asn Lys Tyr Gly Gly Gln Met Lys Pro Thr Trp                 245 250 255 Gly Val Pro Val Glu Glu Ile Gln Arg Gly Ile Lys Asn Gly Val Arg             260 265 270 Lys Val Asn Ile Asp Thr Asp Gly Arg Met Ala Met Thr Gly Gln Ile         275 280 285 Arg Arg Val Leu Gln Glu Asp Pro Ser Glu Phe Asp Pro Arg Lys Tyr     290 295 300 Leu Lys Pro Ala Met Thr Ala Leu Thr Lys Leu Cys Lys Glu Arg Phe 305 310 315 320 Glu Gln Phe Gly Thr Ala Gly Met Ala Gly Arg Ile Thr Pro Leu Pro                 325 330 335 Val Ser Glu Met Ala Lys Arg Tyr Lys Ser Gly Ser Leu Asp Pro Ala             340 345 350 Phe Ser         <210> 67 <211> 354 <212> PRT <213> Rhizobium lupini <400> 67 Met Ala Arg Ile Thr Leu Arg Gln Leu Leu Asp His Ala Ala Glu Glu   1 5 10 15 Gly Tyr Gly Val Ala Phe Asn Ile Asn Asn Met Glu Gln Ala Leu              20 25 30 Ala Ile Met Glu Ala Ala Asp Ala Cys His Ala Pro Val Ile Met Gln          35 40 45 Ala Ser Arg Gly Ala Arg Ala Tyr Ala His Asp Ile Met Leu Lys His      50 55 60 Met Met Asp Ala Val Val Glu Ile Tyr Pro His Ile Pro Val Cys Val  65 70 75 80 His Leu Asp His Gly Asn Asp Pro Ser Asn Cys Met Thr Ala Ile Gln                  85 90 95 Ala Gly Phe Thr Ser Val Met Met Asp Gly Ser Leu Lys Ala Asp Ala             100 105 110 Lys Thr Pro Ala Asp Trp Ala Tyr Asn Val Gly Val Thr Lys Met Val         115 120 125 Thr Asp Met Ala His Phe Gly Gly Ile Ser Val Glu Gly Glu Leu Gly     130 135 140 Val Leu Gly Ser Leu Glu Thr Gly Met Gly Glu Ala Glu Asp Gly His 145 150 155 160 Gly Ala Glu Gly Lys Leu Ser His Asp Gln Leu Leu Thr Asp Pro Asp                 165 170 175 Glu Ala Val Lys Phe Val Arg Glu Thr Lys Val Asp Ala Leu Ala Ile             180 185 190 Ala Met Gly Thr Ser His Gly Ala Tyr Lys Phe Thr Arg Lys Pro Asp         195 200 205 Gly Ser Val Leu Ala Met Asn Val Ile Glu Glu Ile His Arg Lys Leu     210 215 220 Pro Asn Thr His Leu Val Met His Gly Ser Ser Ser Val Pro Ile Glu 225 230 235 240 Leu Gln Glu Ile Ile Asn Lys Tyr Gly Gly Gln Met Lys Pro Thr Trp                 245 250 255 Gly Val Pro Val Glu Glu Ile Gln Arg Gly Ile Lys Asn Gly Val Arg             260 265 270 Lys Val Asn Ile Asp Thr Asp Gly Arg Met Ala Met Thr Gly Gln Ile         275 280 285 Arg Arg Val Leu Gln Glu Asp Pro Ser Glu Phe Asp Pro Arg Lys Tyr     290 295 300 Leu Lys Pro Ala Met Thr Ala Leu Thr Lys Leu Cys Lys Glu Arg Phe 305 310 315 320 Glu Gln Phe Gly Thr Ala Gly Met Ala Gly Arg Ile Thr Pro Leu Pro                 325 330 335 Val Ser Glu Met Ala Lys Arg Tyr Lys Ser Gly Ser Leu Asp Pro Ala             340 345 350 Phe Ser         <210> 68 <211> 354 <212> PRT <213> Rhizobium sp. <400> 68 Met Ala Arg Ile Thr Leu Arg Gln Leu Leu Asp His Ala Ala Glu Glu   1 5 10 15 Gly Tyr Gly Val Ala Phe Asn Ile Asn Asn Met Glu Gln Ala Leu              20 25 30 Ala Ile Met Glu Ala Ala Asp Ala Cys Gln Ala Pro Val Ile Met Gln          35 40 45 Ala Ser Arg Gly Ala Arg Ala Tyr Ala Asn Asp Ile Met Leu Lys His      50 55 60 Met Met Asp Ala Val Val Glu Ile Tyr Pro His Ile Pro Val Cys Val  65 70 75 80 His Leu Asp His Gly Asn Asp Pro Ser Asn Cys Met Thr Ala Ile Gln                  85 90 95 Ala Gly Phe Thr Ser Val Met Met Asp Gly Ser Leu Lys Ala Asp Ala             100 105 110 Lys Thr Pro Ala Asp Trp Ala Tyr Asn Val Gly Val Thr Lys Met Val         115 120 125 Thr Asp Met Ala His Phe Gly Gly Ile Ser Val Glu Gly Glu Leu Gly     130 135 140 Val Leu Gly Ser Leu Glu Thr Gly Met Gly Glu Ala Glu Asp Gly His 145 150 155 160 Gly Ala Glu Gly Lys Leu Ser His Asp Gln Leu Leu Thr Asp Pro Asp                 165 170 175 Glu Ala Val Lys Phe Val Arg Glu Thr Lys Val Asp Ala Leu Ala Ile             180 185 190 Ala Met Gly Thr Ser His Gly Ala Tyr Lys Phe Thr Arg Lys Pro Asp         195 200 205 Gly Ser Val Leu Ala Met Asn Val Ile Glu Glu Ile His Arg Lys Leu     210 215 220 Pro Asn Thr His Leu Val Met His Gly Ser Ser Ser Val Pro Ile Glu 225 230 235 240 Leu Gln Glu Ile Ile Asn Lys Tyr Gly Gly Gln Met Lys Pro Thr Trp                 245 250 255 Gly Val Pro Val Glu Glu Ile Gln Arg Gly Ile Lys Asn Gly Val Arg             260 265 270 Lys Val Asn Ile Asp Thr Asp Gly Arg Met Ala Met Thr Gly Gln Ile         275 280 285 Arg Arg Val Leu Gln Glu Asp Pro Ser Glu Phe Asp Pro Arg Lys Tyr     290 295 300 Leu Lys Pro Ala Met Thr Ala Leu Thr Lys Leu Cys Lys Glu Arg Phe 305 310 315 320 Glu Gln Phe Gly Thr Ala Gly Met Ala Gly Arg Ile Thr Pro Leu Ala                 325 330 335 Val Ser Glu Met Ala Lys Arg Tyr Lys Ser Gly Ser Leu Asp Pro Ala             340 345 350 Phe Ser         <210> 69 <211> 286 <212> PRT <213> Staphylococcus epidermidis <400> 69 Met Pro Leu Val Ser Met Lys Glu Met Leu Ile Asp Ala Lys Glu Asn   1 5 10 15 Gly Tyr Ala Val Gly Gln Tyr Asn Leu Asn Asn Leu Glu Phe Thr Gln              20 25 30 Ala Ile Leu Glu Ala Ser Gln Glu Glu Asn Ala Pro Val Ile Leu Gly          35 40 45 Val Ser Glu Gly Ala Ala Arg Tyr Met Ser Gly Phe Tyr Thr Val Val      50 55 60 Lys Met Val Glu Gly Leu Met His Asp Leu Asn Ile Thr Ile Pro Val  65 70 75 80 Ala Ile His Leu Asp His Gly Ser Ser Phe Glu Lys Cys Lys Glu Ala                  85 90 95 Ile Asp Ala Gly Phe Thr Ser Val Met Ile Asp Ala Ser His Ser Pro             100 105 110 Phe Glu Glu Asn Val Glu Ile Thr Ser Lys Val Val Glu Tyr Ala His         115 120 125 Asp Arg Gly Val Ser Val Glu Ala Glu Leu Gly Thr Val Gly Gly Gln     130 135 140 Glu Asp Val Val Ala Asp Gly Val Ile Tyr Ala Asp Pro Lys Glu 145 150 155 160 Cys Gln Glu Leu Val Glu Lys Thr Gly Ile Asp Thr Leu Ala Pro Ala                 165 170 175 Leu Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Lys Leu Gly Phe             180 185 190 Lys Glu Met Glu Glu Ile Gly Ala Ser Thr Gly Leu Pro Leu Val Leu         195 200 205 His Gly Gly Thr Gly Ile Pro Thr Lys Asp Ile Gln Lys Ala Ile Pro     210 215 220 Tyr Gly Thr Ala Lys Ile Asn Val Asn Thr Glu Asn Gln Ile Ala Ser 225 230 235 240 Ala Lys Ala Val Arg Glu Val Leu Asn Asn Asp Lys Asp Val Tyr Asp                 245 250 255 Pro Arg Lys Tyr Leu Gly Pro Ala Arg Glu Ala Ile Lys Glu Thr Val             260 265 270 Lys Gly Lys Ile Arg Glu Phe Gly Thr Ser Asn Arg Ala Lys         275 280 285 <210> 70 <211> 293 <212> PRT <213> Streptococcus thermophilus <400> 70 Met Ala Ile Val Ser Ala Glu Lys Phe Val Gln Ser Ala Arg Asp Asn   1 5 10 15 Gly Tyr Ala Leu Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Gln              20 25 30 Ala Ile Leu Arg Ala Ala Gla Ala Lys Ala Pro Val Leu Ile Gln          35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Leu Cys Lys      50 55 60 Ala Leu Ile Glu Glu Leu Val Glu Ser Met Gly Ile Thr Val Val  65 70 75 80 Ala Ile His Leu Asp His Gly His Tyr Asp Asp Ala Leu Glu Cys Ile                  85 90 95 Glu Val Gly Tyr Thr Ser Ile Met Phe Asp Gly Ser His Leu Pro Ile             100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Glu Val Val Glu Lys Ala His Ala         115 120 125 Lys Gly Ile Ser Val Glu Ala Glu Val Gly Thr Ile Gly Gly Glu Glu     130 135 140 Asp Gly Ile Val Gly Arg Gly Glu Leu Ala Pro Ile Glu Asp Ala Lys 145 150 155 160 Ala Met Val Ala Thr Gly Val Asp Phe Leu Ala Ala Gly Ile Gly Asn                 165 170 175 Ile His Gly Pro Tyr Pro Glu Asn Trp Glu Gly Leu Asp Leu Asp His             180 185 190 Leu Gln Lys Leu Thr Glu Ala Ile Pro Gly Phe Pro Ile Val Leu His         195 200 205 Gly Gly Ser Gly Ile Pro Asp Asp Gln Ile Gln Glu Ala Ile Lys Leu     210 215 220 Gly Val Ala Lys Val Asn Val Asn Thr Glu Cys Gln Ile Ala Phe Ala 225 230 235 240 Asn Ala Thr Arg Lys Phe Val Ala Glu Tyr Glu Ala Asn Glu Ala Glu                 245 250 255 Tyr Asp Lys Lys Lys Leu Phe Asp Pro Arg Lys Phe Leu Lys Pro Gly             260 265 270 Phe Glu Ala Ile Thr Glu Ala Val Glu Glu Arg Ile Asp Val Phe Gly         275 280 285 Ser Ala Asn Lys Ala     290 <210> 71 <211> 340 <212> PRT <213> Streptomyces avermitilis <400> 71 Met Pro Ile Ala Thr Pro Glu Val Tyr Asn Glu Met Leu Asp Arg Ala   1 5 10 15 Lys Ala Gly Lys Phe Ala Tyr Pro Ala Ile Asn Val Thr Ser Thr Gln              20 25 30 Thr Leu His Ala Ala Leu Arg Gly Phe Ala Glu Ala Glu Ser Asp Gly          35 40 45 Ile Val Gln Ile Ser Thr Gly Gly Ala Glu Phe Leu Gly Gly Gln Tyr      50 55 60 Lys Lys Asp Met Val Thr Gly Ala Val Ala Leu Ala Glu Phe Ala His  65 70 75 80 Ile Val Ala Glu Lys Tyr Pro Val Thr Val Ala Leu His Thr Asp His                  85 90 95 Cys Pro Lys Asp Lys Leu Asp Gly Tyr Val Arg Pro Leu Leu Ala Ile             100 105 110 Ser Glu Glu Arg Val Lys Ala Gly Arg Asn Pro Leu Phe Gln Ser His         115 120 125 Met Trp Asp Gly Ser Ala Glu Thr Leu Ala Asp Asn Leu Ala Ile Ala     130 135 140 Gln Glu Leu Leu Glu Lys Ala Arg Ala Ala Lys Ile Ile Leu Glu Val 145 150 155 160 Glu Ile Thr Pro Thr Gly Gly Glu Glu Asp Gly Val Thr His Glu Ile                 165 170 175 Asn Asp Ser Leu Tyr Thr Thr Val Glu Asp Ala Ile Arg Thr Ala Glu             180 185 190 Ala Leu Gly Leu Gly Glu Lys Gly Arg Tyr Leu Leu Ala Ala Ser Phe         195 200 205 Gly Asn Val His Gly Val Tyr Lys Pro Gly Asn Val Val Leu Arg Pro     210 215 220 Glu Leu Leu Lys Glu Leu Asn Glu Gly Val Ala Lys Phe Gly Lys 225 230 235 240 Ala Asp Pro Phe Asp Phe Val Phe His Gly Gly Ser Gly Ser Thr Glu                 245 250 255 Ala Glu Ile Arg Thr Ala Leu Glu Asn Gly Val Val Lys Met Asn Ile             260 265 270 Asp Thr Asp Thr Gln Tyr Ala Phe Thr Arg Pro Val Ala Asp His Met         275 280 285 Phe Arg Asn Tyr Asp Gly Val Leu Lys Val Asp Gly Glu Val Gly Asp     290 295 300 Lys Lys Thr Tyr Asp Pro Arg Thr Trp Gly Lys Leu Ala Glu Ala Ser 305 310 315 320 Met Ala Lys Arg Val Thr Glu Ala Cys Ala Asn Leu Arg Ser Thr Gly                 325 330 335 Thr Lys Ile Lys             340 <210> 72 <211> 294 <212> PRT <213> Streptomyces viridochromogenes <400> 72 Met Thr Ile Asp Pro Gly Asp Thr Val Pro Leu Thr Thr Thr Gly Glu   1 5 10 15 Leu Val Thr Arg Ala Ala Ala Arg Ala Ala Val Ala Ser Phe Asn              20 25 30 Val Ile Thr Leu Glu His Val Glu Ala Val Ile Ala Gly Ala Glu Ser          35 40 45 Val Pro Ala Pro Val Val Leu Gln Val Ser Glu Asn Ala Val Arg Phe      50 55 60 Arg Gly Gly Arg Leu Leu Pro Leu Ala Arg Ala Ala Val Thr Ala Ala  65 70 75 80 Glu His Ala Asp Val Pro Ala Leu His Leu Asp His Val Gln Ser                  85 90 95 Glu Ala Leu Leu His Gln Ala Ala Asp Val Gly Phe Ser Ser Val Met             100 105 110 Tyr Asp Ala Ala Arg Leu Pro Tyr Ala Glu Asn Leu Ala Ala Thr Arg         115 120 125 Ala Ala Val Asp Trp Ala His Ala Arg Gly Leu Trp Ile Glu Ala Glu     130 135 140 Leu Gly Gln Ile Gly Gly Lys Asn Gly Arg Pro Pro Leu Asp Ala His 145 150 155 160 Ala Pro Gly Ala Arg Thr Asp Pro Asp Glu Ala Arg Asp Phe Val Ala                 165 170 175 Asp Ser Gly Val Asp Ala Leu Ala Val Ala Ile Gly Ser Ala His Ala             180 185 190 Met Thr Thr Arg Thr Ala Ala Leu Asp His Ala Leu Leu Lys Gln Leu         195 200 205 Ser Ala Leu Asp Val Pro Leu Val Leu His Gly Ser Ser Gly Val     210 215 220 Pro Asp Gly Glu Leu Thr Ala Ala Val Ala Gly Gly Ile Thr Lys Val 225 230 235 240 Asn Ile Gly Thr Ala Leu Asn Val Ala Leu Thr Gly Ala Ile Arg Asp                 245 250 255 Phe Leu Ala Asp Arg Pro Glu Ala Val Asp Pro Arg Gly Tyr Leu Thr             260 265 270 Val Ala Arg Glu Ala Met Thr Arg Ala Val Ala Arg Ala Leu Gln Val         275 280 285 Leu Gly Ala Thr Ile Ala     290 <210> 73 <211> 299 <212> PRT <213> Bacillus subtilis <400> 73 Met Leu Gly Gly Ily Gly Ala Gly Gly Thr Lys Phe Val Cys Ala Val   1 5 10 15 Gly Arg Glu Asp Gly Thr Ile Ile Asp Arg Ile Glu Phe Pro Thr Lys              20 25 30 Met Pro Asp Glu Thr Ile Glu Lys Val Ile Gln Tyr Phe Ser Gln Phe          35 40 45 Ser Leu Gln Ala Ile Gly Ile Gly Ser Phe Gly Pro Val Asp Asn Asp      50 55 60 Lys Thr Ser Gln Thr Tyr Gly Thr Ile Thr Ala Thr Pro Lys Ala Gly  65 70 75 80 Trp Arg His Tyr Pro Phe Leu Gln Thr Val Lys Asn Glu Met Lys Ile                  85 90 95 Pro Val Gly Phe Ser Thr Asp Val Asn Ala Ala Leu Gly Glu Phe             100 105 110 Leu Phe Gly Glu Ala Lys Gly Leu Asp Ser Cys Leu Tyr Ile Thr Ile         115 120 125 Gly Thr Gly Ile Gly Ala Gly Ala Ile Val Glu Gly Arg     130 135 140 Gly Leu Ser His Pro Glu Met Gly His Ile Tyr Ile Arg Arg His Pro 145 150 155 160 Asp Asp Val Tyr Gln Gly Lys Cys Pro Tyr His Gly Asp Cys Phe Glu                 165 170 175 Gly Leu Ala Ser Gly Pro Ala Ile Glu Ala Arg Trp Gly Lys Lys Ala             180 185 190 Ala Asp Leu Ser Asp Ile Ala Gln Val Trp Glu Leu Glu Gly Tyr Tyr         195 200 205 Ile Ala Gln Ala Leu Ala Gln Tyr Ile Leu Ile Leu Ala Pro Lys Lys     210 215 220 Ile Ile Leu Gly Gly Gly Val Met Gln Gln Lys Gln Val Phe Ser Tyr 225 230 235 240 Ile Tyr Gln Tyr Val Pro Lys Ile Met Asn Ser Tyr Leu Asp Phe Ser                 245 250 255 Glu Leu Ser Asp Asp Ile Ser Asp Tyr Ile Val Pro Pro Arg Leu Gly             260 265 270 Ser Asn Ala Gly Ile Ile Gly Thr Leu Val Leu Ala His Gln Ala Leu         275 280 285 Gln Ala Glu Ala Ala Ser Gly Glu Val Arg Ser     290 295 <210> 74 <211> 307 <212> PRT <213> Escherichia coli <400> 74 Met Ser Ala Lys Val Trp Val Leu Gly Asp Ala Val Val Asp Leu Leu   1 5 10 15 Pro Glu Ser Asp Gly Arg Leu Leu Pro Cys Pro Gly Gly Ala Pro Ala              20 25 30 Asn Val Ala Val Gly Ile Ala Arg Leu Gly Gly Thr Ser Gly Phe Ile          35 40 45 Gly Arg Val Gly Asp Asp Pro Phe Gly Ala Leu Met Gln Arg Thr Leu      50 55 60 Leu Thr Glu Gly Val Asp Ile Thr Tyr Leu Lys Gln Asp Glu Trp His  65 70 75 80 Arg Thr Ser Thr Val Leu Val Asp Leu Asn Asp Gln Gly Glu Arg Ser                  85 90 95 Phe Thr Phe Met Val Arg Pro Ser Ala Asp Leu Phe Leu Glu Thr Thr             100 105 110 Asp Leu Pro Cys Trp Arg His Gly Glu Trp Leu His Leu Cys Ser Ile         115 120 125 Ala Leu Ser Ala Glu Pro Ser Arg Thr Ser Ala Phe Thr Ala Met Thr     130 135 140 Ala Ile Arg His Ala Gly Gly Phe Val Ser Phe Asp Pro Asn Ile Arg 145 150 155 160 Glu Asp Leu Trp Gln Asp Glu His Leu Leu Arg Leu Cys Leu Arg Gln                 165 170 175 Ala Leu Gln Leu Ala Asp Val Val Lys Leu Ser Glu Glu Glu Trp Arg             180 185 190 Leu Ile Ser Gly Lys Thr Gln Asn Asp Arg Asp Ile Cys Ala Leu Ala         195 200 205 Lys Glu Tyr Glu Ile Ala Met Leu Leu Val Thr Lys Gly Ala Glu Gly     210 215 220 Val Val Cys Tyr Arg Gly Gln Val His His Phe Ala Gly Met Ser 225 230 235 240 Val Asn Cys Val Asp Ser Thr Gly Ala Gly Asp Ala Phe Val Ala Gly                 245 250 255 Leu Leu Thr Gly Leu Ser Ser Thr Gly Leu Ser Thr Asp Glu Arg Glu             260 265 270 Met Arg Arg Ile Ile Asp Leu Ala Gln Arg Cys Gly Ala Leu Ala Val         275 280 285 Thr Ala Lys Gly Ala Met Thr Ala Leu Pro Cys Arg Gln Glu Leu Glu     290 295 300 Ser Glu Lys 305 <210> 75 <211> 302 <212> PRT <213> Escherichia coli <400> 75 Met Arg Ile Gly Ile Asp Leu Gly Gly Thr Lys Thr Glu Val Ile Ala   1 5 10 15 Leu Gly Asp Ala Gly Glu Gln Leu Tyr Arg His Arg Leu Pro Thr Pro              20 25 30 Arg Asp Asp Tyr Arg Gln Thr Ile Glu Thr Ile Ala Thr Leu Val Asp          35 40 45 Met Ala Glu Gln Ala Thr Gly Gln Arg Gly Thr Val Gly Met Gly Ile      50 55 60 Pro Gly Ser Ile Ser Pro Thyr Gly Val Val Lys Asn Ala Asn Ser  65 70 75 80 Thr Trp Leu Asn Gly Gln Pro Phe Asp Lys Asp Leu Ser Ala Arg Leu                  85 90 95 Gln Arg Glu Val Arg Leu Ala Asn Asp Ala Asn Cys Leu Ala Val Ser             100 105 110 Glu Ala Val Asp Gly Ala Ala Gly Ala Gln Thr Val Phe Ala Val         115 120 125 Ile Ile Gly Thr Gly Cys Gly Ala Gly Val Ala Phe Asn Gly Arg Ala     130 135 140 His Ile Gly Gly Asn Gly Thr Ala Gly Glu Trp Gly His Asn Pro Leu 145 150 155 160 Pro Trp Met Asp Glu Asp Glu Leu Arg Tyr Arg Glu Glu Val Pro Cys                 165 170 175 Tyr Cys Gly Lys Gln Gly Cys Ile Glu Thr Phe Ile Ser Gly Thr Gly             180 185 190 Phe Ala Met Asp Tyr Arg Arg Leu Ser Gly His Ala Leu Lys Gly Ser         195 200 205 Glu Ile Ile Arg Leu Val Glu Glu Ser Asp Pro Val Ala Glu Leu Ala     210 215 220 Leu Arg Arg Tyr Glu Leu Arg Leu Ala Lys Ser Leu Ala His Val Val 225 230 235 240 Asn Ile Leu Asp Pro Asp Val Ile Val Leu Gly Gly Gly Met Ser Asn                 245 250 255 Val Asp Arg Leu Tyr Gln Thr Val Gly Gln Leu Ile Lys Gln Phe Val             260 265 270 Phe Gly Gly Glu Cys Glu Thr Pro Val Arg Lys Ala Lys His Gly Asp         275 280 285 Ser Ser Gly Val Arg Gly Ala Ala Trp Leu Trp Pro Gln Glu     290 295 300 <210> 76 <211> 291 <212> PRT <213> Fusobacterium mortiferum <400> 76 Met Lys Phe Gly Ala Ile Asp Ala Gly Gly Lys Lys Phe Ile Cys Gly   1 5 10 15 Ile Thr Asp Glu Gln Gly Asn Thr Leu Glu Lys Thr Ser Phe Leu Thr              20 25 30 Glu Thr Pro Glu Lys Thr Ile Pro Leu Val Ile Asp Phe Phe Arg Asn          35 40 45 Lys Asp Ile Ser Ala Leu Gly Val Gly Cys Phe Gly Pro Leu Asp Leu      50 55 60 Asn Pro Asn Ser Asp Thr Tyr Gly Tyr Ile Thr Ser Ser Pro Lys Thr  65 70 75 80 Asp Trp Lys Asn Tyr Asn Ile Leu Gly Asn Leu Lys Ser Ala Phe Asn                  85 90 95 Ile Pro Ile Phe Leu Asp Thr Asn Met Asn Val Gly Val Leu Gly Glu             100 105 110 Ala Met Trp Gly Ala Gly Lys Gly Leu Lys Asn Thr Ile Phe Leu Thr         115 120 125 Ile Gly Asn Gly Ily Gly Ily Gle Ily Val Glu Gly Lys Ile Val     130 135 140 His Gly Met Leu His Pro Glu Met Gly His Met Phe Val Ser Arg His 145 150 155 160 Pro Arg Asp Lys Phe Ala Gly Thr Cys Pro Phe His Gly Gly Asn Cys                 165 170 175 Leu Glu Gly Met Ala Ser Val Pro Ala Ile Glu Lys Arg Trp Gly Lys             180 185 190 Ser Leu Ser Ser Leu Pro Ile Glu His Pro Ala Trp Asp Leu Glu Ala         195 200 205 Phe Tyr Ile Ala His Ala Leu Val Asn Tyr Ile Leu Ile Leu Ser Pro     210 215 220 Glu Lys Ile Ile Leu Gly Gly Glu Val Met Lys Tyr Lys Gln Leu Leu 225 230 235 240 Pro Ile Val Lys Lys Ala Val Ile Lys Leu Leu Asn Gly Tyr Ile Gln                 245 250 255 Thr Asn Gln Ile Phe Arg Glu Ile Asp Asp Tyr Ile Val Leu Pro Asp             260 265 270 Leu Gly Glu Asn Ser Gly Phe Phe Gly Ala Ala Ala Leu Cys Ile Lys         275 280 285 Asn Phe Lys     290 <210> 77 <211> 307 <212> PRT <213> Klebsiella pneumoniae <400> 77 Met Asn Gly Lys Ile Trp Val Leu Gly Asp Ala Val Val Asp Leu Leu   1 5 10 15 Pro Asp Gly Glu Gly Arg Leu Leu Gln Cys Pro Gly Gly Ala Pro Ala              20 25 30 Asn Val Ala Val Gly Val Ala Arg Leu Gly Asp Ser Gly Phe Ile          35 40 45 Gly Arg Val Gly Asp Asp Pro Phe Gly Arg Phe Met Arg His Thr Leu      50 55 60 Ala Gln Glu Gln Val Asp Val Asn Tyr Met Arg Leu Asp Ala Ala Gln  65 70 75 80 Arg Thr Ser Thr Val Val Val Asp Leu Asp Ser His Gly Glu Arg Thr                  85 90 95 Phe Thr Phe Met Val Arg Pro Ser Ala Asp Leu Phe Leu Gln Pro Glu             100 105 110 Asp Leu Pro Pro Phe Ala Ala Gly Gln Trp Leu His Val Cys Ser Ile         115 120 125 Ala Leu Ser Ala Glu Pro Ser Ser Ser Thr Thr Phe Ala Ala Leu Glu     130 135 140 Ala Ile Lys Arg Ala Gly Gly Tyr Val Ser Phe Asp Pro Asn Ile Arg 145 150 155 160 Ser Asp Leu Trp Gln Asp Pro Gln Asp Leu Arg Asp Cys Leu Asp Arg                 165 170 175 Ala Leu Ala Leu Ala Asp Ala Ile Lys Leu Ser Glu Glu Glu Leu Ala             180 185 190 Phe Ile Ser Gly Ser Asp Asp Ile Val Ser Gly Ile Ala Arg Leu Asn         195 200 205 Ala Arg Phe Gln Pro Thr Leu Leu Leu Val Thr Gln Gly Lys Ala Gly     210 215 220 Val Gln Ala Leu Arg Gly Gln Val Ser His Phe Pro Ala Arg Pro 225 230 235 240 Val Val Ala Val Asp Thr Thr Gly Ala Gly Asp Ala Phe Val Ala Gly                 245 250 255 Leu Leu Ala Gly Leu Ala Ala His Gly Ile Pro Asp Asn Leu Ala Ala             260 265 270 Leu Ala Pro Asp Leu Ala Leu Ala Gln Thr Cys Gly Ala Leu Ala Thr         275 280 285 Thr Ala Lys Gly Ala Met Thr Ala Leu Pro Tyr Lys Asp Asp Leu Gln     290 295 300 Arg Ser Leu 305 <210> 78 <211> 290 <212> PRT <213> Lactococcus lactis subsp. cremoris <400> 78 Met Ser Val Tyr Tyr Gly Ser Ile Glu Ala Gly Gly Thr Lys Phe Val   1 5 10 15 Leu Ala Ile Ala Asp Glu His Phe Asn Ile Ile Lys Lys Phe Lys Phe              20 25 30 Ala Thr Thr Thr Pro Gln Glu Thr Ile Ser Lys Thr Ile Lys Tyr Phe          35 40 45 Lys Glu Asn Arg Val Ser Ala Ile Gly Leu Gly Ser Phe Gly Pro Ile      50 55 60 Asp Leu Asn Leu Ser Ser Lys Thr Tyr Gly Tyr Ile Thr Ser Thr Pro  65 70 75 80 Lys Val Gly Trp Lys Asn Ile Asn Leu Val Gly Gln Leu Lys Glu Ala                  85 90 95 Leu Asp Ile Pro Ile Tyr Phe Thr Thr Asp Val Asn Ala Ser Ala Tyr             100 105 110 Gly Glu Met Lys Asn Thr Gly Ile Lys Asn Leu Val Tyr Leu Thr Ile         115 120 125 Gly Thr Gly Ily Gly Gly Gly Ala Ile Gln Asn Gly Tyr Phe Ile Gly     130 135 140 Gly Ile Gly His Ser Glu Met Gly His Gln Arg Ile Asn Arg His Arg 145 150 155 160 Asp Val Leu Thr Phe Glu Gly Ile Cys Pro Phe His Gly Asp Cys Leu                 165 170 175 Glu Gly Val Ala Ala Gly Pro Ser Leu Glu Ala Arg Thr Gly Ile Leu             180 185 190 Gly Glu Lys Ile Ser Ser Asp Asp Pro Ile Trp Asp Ile Leu Ser Tyr         195 200 205 Tyr Ile Ala Gln Ala Ala Ile Asn Ala Thr Leu Thr Leu Ala Pro Glu     210 215 220 Cys Ile Ile Leu Gly Gly Gly Val Met Glu Lys Pro Asn Met Ile Ser 225 230 235 240 Leu Ile Gln Lys Gln Phe Ile Ser Met Leu Asn Asn Tyr Ile Asp Leu                 245 250 255 Pro Cys Ser Val Glu Lys Tyr Ile Arg Leu Pro Thr Val Lys Glu Asn             260 265 270 Gly Ser Ala Thr Leu Gly Asn Phe Tyr Leu Ala Tyr Ser Leu Phe Thr         275 280 285 Lys Glu     290 <210> 79 <211> 291 <212> PRT <213> Lactococcus lactis subsp. lactis <400> 79 Met Thr Asn His Tyr Gly Ser Ile Glu Ala Gly Gly Thr Lys Phe Val   1 5 10 15 Leu Ala Ile Gly Asp Glu Gln Phe Asn Ile Leu Lys Thr Asn Gln Ile              20 25 30 Pro Thr Thr Ser Pro Glu Glu Thr Ile Thr Arg Thr Ile Glu Phe Phe          35 40 45 Lys Glu Asn Pro Val Lys Ser Leu Ala Val Gly Ser Phe Gly Pro Ile      50 55 60 Asp Ile Lys Pro Glu Ser Ala Thr Tyr Gly Tyr Ile Thr Thr Thr Pro  65 70 75 80 Lys Ala Gly Trp Ala Asn Val Asp Leu Leu Gly Lys Leu Lys Ala Ala                  85 90 95 Leu Asp Ile Pro Met Val Phe Thr Thr Asp Val Asn Ser Ser Ala Tyr             100 105 110 Gly Glu Met Ile Val Thr Gly Gly Lys Ser Leu Val Tyr Phe Thr Ile         115 120 125 Gly Thr Gly Ile Gly Gly Gly Aly Val Gln Asp Gly Lys Phe Ile Gly     130 135 140 Gly Phe Ser His Ala Glu Met Gly His Gln Tyr Val Lys Arg His Pro 145 150 155 160 Asp Asp Leu Asn Phe Ala Gly Val Cys Pro Phe His Gly Asp Cys Leu                 165 170 175 Glu Gly Val Ala Ala Gly Pro Ser Leu Glu Ala Arg Leu Gly Ile Arg             180 185 190 Gly Glu Asp Ile Pro Ala Asp Ser Lys Val Trp Asp Ile Gln Ala Phe         195 200 205 Tyr Ile Ala Gln Ile Ala Val Asn Thr Thr Leu Ala Leu Ala Pro Glu     210 215 220 Lys Ile Val Phe Gly Gly Gly Val Met Thr Gln Thr His Met Ile Glu 225 230 235 240 Arg Val Arg Lys Glu Phe Thr Lys Leu Met Ala Gly Tyr Val His Val                 245 250 255 Pro Tyr Leu Leu Thr Asp Tyr Ile Met Thr Pro Ser Val Ala Asn Asn             260 265 270 Gly Ser Ala Thr Ile Gly Asn Phe Ala Leu Ala Lys Asp Leu Asp Glu         275 280 285 Lys Asn Tyr     290 <210> 80 <211> 288 <212> PRT <213> Pediococcus pentosaceus <400> 80 Met Leu Leu Gly Ala Ile Glu Ala Gly Gly Thr Lys Phe Val Cys Ala   1 5 10 15 Thr Gly Ala Glu Asn Gly Gln Val Ser Asp Arg Ile Ser Ile Pro Thr              20 25 30 Thr Thr Pro Val Glu Thr Met Thr Ala Val Asp Asp Tyr Phe Thr Thr          35 40 45 His Pro Val Asp Ile Gly Ile Gly Ser Phe Gly Pro Ile Gly Val      50 55 60 Asn Pro His Asp Pro Lys Tyr Gly Tyr Ile Thr Thr Pro Lys Pro  65 70 75 80 Gly Trp Gly Asp Phe Asp Phe Leu Gly His Leu Lys Ser Gln Phe Asn                  85 90 95 Ile Pro Leu Tyr Trp Thr Thr Asp Val Asn Glu Ala Ala Tyr Gly Glu             100 105 110 Ser Met Ile Gly Ile Ala Lys Asp Val Pro Asn Ser Ile Tyr Met Thr         115 120 125 Ile Gly Thr Gly Val Gly Ala Gly Val Ile Ser Gln Asn His Ile Phe     130 135 140 Asn Gly Arg Thr His Thr Glu Leu Gly His Met Arg Leu Asn Arg Leu 145 150 155 160 Pro Gly Asp Asp Phe Lys Ser Asn Cys Pro Tyr His Asp Ile Cys Leu                 165 170 175 Glu Gly Leu Ala Ala Gly Ala Val Gly Lys Arg Thr Gly Lys Ala             180 185 190 Gly Lys Asp Ile Pro Val Asp Asp Pro Val Trp Pro Ile Ile Thr Asp         195 200 205 Tyr Ile Ala Gln Ala Cys Val Asn Leu Thr Val Ala Phe Ala Pro Asp     210 215 220 Lys Ile Ile Leu Asn Gly Gly Val Met Asn Gln Arg Gln Leu Phe Pro 225 230 235 240 Met Ile Arg Glu Lys Phe Ala Ala Tyr Leu Asn Gly Tyr Glu Glu Val                 245 250 255 Pro Pro Leu Asp Asp Tyr Ile Val Pro Ala Gly Leu Gly Asn Asn Ser             260 265 270 Gly Ile Ala Gly Gly Leu Leu Leu Ala Gln Ala Ala Leu Lys Asn Ala         275 280 285 <210> 81 <211> 326 <212> PRT <213> Rhizobium leguminosarum bv. trifoli <400> 81 Met Ile Leu Cys Cys Gly Glu Ala Leu Ile Asp Met Leu Pro Arg Asp   1 5 10 15 Thr Thr Leu Gly Gly Lys Gly Ply Ala Pro Tyr Ala Gly Gly Ala Ile              20 25 30 Phe Asn Thr Ala Ile Ala Leu Gly Arg Leu Gly Ile Pro Thr Ala Phe          35 40 45 Phe Thr Gly Ile Ale Asp Asp Met Met Gly Glu Ile Leu Leu Glu Thr      50 55 60 Leu Lys Ala Ser Asn Val Asp Tyr Ser Pro Cys Ala Ile Thr Pro Arg  65 70 75 80 Pro Ser Thr Ile Ala Phe Val Lys Leu Val Asn Gly Gln Ala Thr Tyr                  85 90 95 Ala Phe Tyr Asp Glu Gly Thr Ala Gly Arg Met Ile Thr Thr Ala Asp             100 105 110 Leu Pro Asp Leu Gly Asp Asp Cys Glu Ala Leu His Phe Gly Ala Ile         115 120 125 Ser Leu Ile Pro Ser Pro Cys Gly Glu Thr Tyr Glu Ala Leu Leu Asp     130 135 140 Arg Glu Ala Ala Ser Arg Val Ile Ser Leu Asp Pro Asn Ile Arg Pro 145 150 155 160 Gly Phe Ile Lys Asp Lys Pro Ser His Met Ala Arg Ile Lys Arg Met                 165 170 175 Ala Ala Lys Ser Asp Ile Val Lys Phe Ser Asp Glu Asp Leu Asp Trp             180 185 190 Phe Gly Leu Gln Gly Asp His Asp Ala Leu Ala Ala His Trp Leu Asn         195 200 205 His Gly Ala Lys Leu Val Val Ile Thr Lys Gly Ala Glu Gly Ala Ser     210 215 220 Gly Tyr Thr Lys Asp Arg Lys Val Thr Val Ser Ser Glu Arg Val Thr 225 230 235 240 Val Val Asp Thr Val Gly Ala Gly Asp Thr Phe Asp Ala Gly Ile Leu                 245 250 255 Ala Ser Leu Lys Met Asp Asn Leu Leu Thr Lys Arg Gln Val Ala Ser             260 265 270 Leu Asp Glu Gln Ala Leu Arg Asn Gly Pro Asp Pro Arg Arg Gln Ser         275 280 285 Arg Arg Arg His Arg Leu Pro Arg Arg Arg Gln Ser Thr Leu Gly Ala     290 295 300 Arg Asp Trp Ser Leu Arg Leu Glu Gln Asp Ser Asp Pro His Pro Pro 305 310 315 320 Asp Asp Thr Phe Ser Pro                 325 <210> 82 <211> 221 <212> PRT <213> Salmonella thompson <400> 82 Val Asp Leu Asp Asp Gln Gly Glu Arg Thr Phe Thr Phe Met Val Arg   1 5 10 15 Pro Ser Ala Asp Leu Phe Leu Val Glu Glu Asp Leu Pro Gln Phe Ala              20 25 30 Ala Gly Gln Trp Leu His Val Cys Ser Ile Ala Leu Ser Ala Glu Pro          35 40 45 Ser Ser Thr Thr Phe Ala Ala Met Glu Ser Ile Arg Ser Ala Gly      50 55 60 Gly Arg Val Ser Phe Asp Pro Asn Ile Arg Pro Asp Leu Trp Gln Asp  65 70 75 80 Gln Ala Leu Leu Leu Ala Cys Leu Asp Arg Ala Leu His Met Ala Asn                  85 90 95 Val Val Lys Leu Ser Glu Glu Glu Leu Val Phe Ile Ser Ser Sern             100 105 110 Asp Leu Ala Tyr Gly Ile Ala Ser Val Thr Glu Arg Tyr Gln Pro Glu         115 120 125 Leu Leu Val Thr Arg Gly Lys Ala Gly Val Leu Ala Ala Phe Gln     130 135 140 Gln Lys Phe Thr His Phe Asn Ala Arg Pro Val Ala Ser Val Asp Thr 145 150 155 160 Thr Gly Ala Gly Asp Ala Phe Val Ala Gly Leu Leu Ala Ser Leu Ala                 165 170 175 Ala Asn Gly Met Pro Thr Asp Met Thr Ala Leu Glu Pro Thr Leu Thr             180 185 190 Leu Ala Gln Thr Cys Gly Ala Leu Ala Thr Ala Lys Gly Ala Met         195 200 205 Thr Ala Leu Pro Tyr Gln Arg Asp Leu Asn Arg Gln Phe     210 215 220 <210> 83 <211> 307 <212> PRT <213> Salmonella typhimurium <400> 83 Met Asn Ala Lys Val Trp Val Leu Gly Asp Ala Val Val Asp Leu Leu   1 5 10 15 Pro Glu Ser Glu Gly Arg Leu Leu Gln Cys Pro Gly Gly Ala Pro Ala              20 25 30 Asn Val Ala Val Gly Val Ala Arg Leu Gly Gly Asn Ser Gly Phe Ile          35 40 45 Gly Ala Val Gly Gly Asp Pro Phe Gly Arg Tyr Met Arg His Thr Leu      50 55 60 Gln Gln Glu Gln Val Asp Val Ser His Met Tyr Leu Asp Asp Gln His  65 70 75 80 Arg Thr Ser Thr Val Val Val Asp Leu Asp Asp Gln Gly Glu Arg Thr                  85 90 95 Phe Thr Phe Met Val Arg Pro Ser Ala Asp Leu Phe Leu Val Glu Glu             100 105 110 Asp Leu Pro Gln Phe Ala Ala Gly Gln Trp Leu His Val Cys Ser Ile         115 120 125 Ala Leu Ser Ala Glu Pro Ser Ser Ser Thr Thr Phe Ala Ala Met Glu     130 135 140 Ser Ile Arg Ser Ala Gly Gly Arg Val Ser Phe Asp Pro Asn Ile Arg 145 150 155 160 Pro Asp Leu Trp Gln Asp Gln Ala Leu Leu Leu Ala Cys Leu Asp Arg                 165 170 175 Ala Leu His Met Ala Asn Val Val Lys Leu Ser Glu Glu Glu Leu Val             180 185 190 Phe Ile Ser Ser Asn Asp Leu Ala Tyr Gly Ile Ala Ser Val Thr         195 200 205 Glu Arg Tyr Gln Pro Glu Leu Leu Leu Val Thr Arg Gly Lys Ala Gly     210 215 220 Val Leu Ala Ala Phe Gln Gln Lys Phe Thr His Phe Asn Ala Arg Pro 225 230 235 240 Val Ala Ser Val Asp Thr Thr Gly Ala Gly Asp Ala Phe Val Ala Gly                 245 250 255 Leu Leu Ala Ser Leu Ala Ala Asn Gly Met Pro Thr Asp Met Thr Ala             260 265 270 Leu Glu Pro Thr Leu Thr Leu Ala Gln Thr Cys Gly Ala Leu Ala Thr         275 280 285 Thr Ala Lys Gly Ala Met Thr Ala Leu Pro Tyr Gln Arg Asp Leu Asn     290 295 300 Arg Gln Phe 305 <210> 84 <211> 293 <212> PRT <213> Streptococcus mutans serotype c <400> 84 Met Ser Lys Leu Tyr Gly Ser Ile Glu Ala Gly Gly Thr Lys Phe Val   1 5 10 15 Cys Ala Val Gly Asp Glu Asn Phe Gln Ile Leu Glu Lys Val Gln Phe              20 25 30 Pro Thr Thr Pro Tyr Glu Thr Ile Glu Lys Thr Val Ala Phe Phe          35 40 45 Lys Lys Phe Glu Ala Asp Leu Ala Ser Val Ala Ile Gly Ser Phe Gly      50 55 60 Pro Ile Asp Ile Asp Gln Asn Ser Asp Thr Tyr Gly Tyr Ile Thr Ser  65 70 75 80 Thr Pro Lys Pro Asn Trp Ala Asn Val Asp Phe Val Gly Leu Ile Ser                  85 90 95 Lys Asp Phe Lys Ile Pro Phe Tyr Phe Thr Thr Asp Val Asn Ser Ser             100 105 110 Ala Tyr Gly Glu Thr Ile Ala Arg Ser Asn Val Lys Ser Leu Val Tyr         115 120 125 Tyr Thr Ile Gly Thr Gly Ile Gly Ala Gly Thr Ile Gln Asn Gly Glu     130 135 140 Phe Ile Gly Gly Met Gly His Thr Glu Ala Gly His Val Tyr Met Ala 145 150 155 160 Pro His Pro Asn Asp Val His His Gly Phe Val Gly Thr Cys Pro Phe                 165 170 175 His Lys Gly Cys Leu Glu Gly Leu Ala Ala Gly Pro Ser Leu Glu Ala             180 185 190 Arg Thr Gly Ile Arg Gly Glu Leu Ile Glu Gln Asn Ser Glu Val Trp         195 200 205 Asp Ile Gln Ala Tyr Tyr Ile Ala Gln Ala Ila Ile Gln Ala Thr Val     210 215 220 Leu Tyr Arg Pro Gln Val Ile Val Phe Gly Gly Gly Val Ala Gln 225 230 235 240 Glu His Met Leu Asn Arg Val Glu Lys Phe Thr Ser Leu Leu Asn                 245 250 255 Asp Tyr Leu Pro Val Pro Asp Val Lys Asp Tyr Ile Val Thr Pro Ala             260 265 270 Val Ala Glu Asn Gly Ser Ala Thr Leu Gly Asn Leu Ala Leu Ala Lys         275 280 285 Lys Ile Ala Ala Arg     290 <210> 85 <211> 307 <212> PRT <213> Vibrio alginolyticus <400> 85 Met Asn Gln Val Trp Val Thr Gly Asp Ala Val Val Asp Leu Ile Pro   1 5 10 15 Glu Ser Glu Thr Ser Leu Leu Lys Cys Pro Gly Gly Ala Pro Ala Asn              20 25 30 Val Ala Val Ala Ile Ala Arg Leu Ser Gly Lys Ser Ala Phe Phe Gly          35 40 45 Arg Val Gly Asp Asp Pro Phe Gly Arg Phe Met Gln Ser Ile Leu Asp      50 55 60 Gln Glu Gly Val Cys Thr Glu Phe Leu Ile Lys Asp Pro Glu Gln Arg  65 70 75 80 Thr Ser Thr Val Val Val Asp Leu Asp Asp Gln Gly Glu Arg Ser Phe                  85 90 95 Thr Phe Met Val Lys Pro Ser Ala Asp Gln Phe Met Ser Val Glu Asp             100 105 110 Met Gly Asn Phe Lys Gln Gly Asp Trp Leu His Val Cys Ser Ser Ser         115 120 125 Leu Ala Asn Glu Pro Ser Arg Ser Ser Thr Phe Glu Ala Ile Lys Arg     130 135 140 Ala Lys Ala Ala Gly Gly Phe Ile Ser Phe Asp Pro Asn Leu Arg Asp 145 150 155 160 Glu Val Trp Gln Asp Gln Ser Glu Ile Gln Ala Val Val Met Lys Ala                 165 170 175 Val Ala Met Ala Val Val Lys Phe Ser Glu Glu Glu Leu Leu Phe             180 185 190 Leu Thr Asp Ser Thr Ser Ala Gln Gly Leu Gln Gln Ile Ala Ala         195 200 205 Met Asn Ile Ala Leu Val Leu Val Thr Gln Gly Ala Lys Gly Val Trp     210 215 220 Arg Val Phe Glu Ser Gln Ser Glu Leu Ile Thr Gly Gln Val Ser Ser 225 230 235 240 Pro Ile Asp Thr Thr Gly Ala Gly Asp Ala Phe Val Gly Gly Leu Leu                 245 250 255 Ala Cys Leu Ser Arg His Ala Asp Trp Lys Asn His Pro Val Val Ser             260 265 270 Ser Ala Ile Gln Trp Ala Asn Gly Cys Gly Ala Leu Ala Thr Thr Gln         275 280 285 Lys Gly Ala Met Thr Ala Leu Pro Thr Gln Thr Glu Leu Leu Arg Phe     290 295 300 Ile Gly Gln 305 <210> 86 <211> 301 <212> PRT <213> Zymomonas mobilis subsp. mobilis <400> 86 Met Lys Asn Asp Lys Lys Ile Tyr Gly Cys Ile Glu Gly Gly Gly Thr   1 5 10 15 Lys Phe Met Leu Ala Leu Ile Asp Ser Asp Arg Lys Met Leu Ala Val              20 25 30 Glu Arg Val Pro Thr Thr Thr Pro Glu Glu Thr Leu Gly Lys Ser Val          35 40 45 Glu Phe Phe Lys Lys Ala Leu Pro Gln Tyr Ala Asp Ser Phe Ala Ser      50 55 60 Phe Gly Ile Ala Ser Phe Gly Pro Leu Cys Leu Asp Arg Lys Ser Pro  65 70 75 80 Lys Trp Gly Tyr Ile Thr Asn Thr Pro Lys Pro Phe Trp Pro Asn Thr                  85 90 95 Asp Val Val Thr Pro Phe Lys Glu Ala Phe Gly Cys Pro Val Glu Ile             100 105 110 Asp Thr Asp Val Asn Gly Ala Ala Leu Ala Glu Asn Phe Trp Gly Ala         115 120 125 Ser Lys Gly Thr His Thr Ser Val Tyr Val Thr Val Gly Thr Gly Phe     130 135 140 Gly Gly Gly Val Leu Ile Asp Gly Lys Pro Ile His Gly Leu Ala His 145 150 155 160 Pro Glu Met Gly His Gly Ile Pro Ile Arg His Pro Asp Asp Arg Asp                 165 170 175 Phe Glu Gly Cys Cys Pro Tyr His Gly Gly Cys Tyr Glu Gly Leu Ala             180 185 190 Ser Gly Thr Ala Ile Arg Lys Arg Trp Gly Lys Ala Leu Asn Glu Met         195 200 205 Glu Pro Ala Glu Phe Glu Lys Ala Arg Glu Ile Ile Ala Phe Tyr Leu     210 215 220 Ala His Phe Asn Val Thr Leu Gln Ala Phe Ile Ser Pro Glu Arg Ile 225 230 235 240 Val Phe Gly Gly Gly Val Met His Val Val Asp Gly Met Leu Ala Ser Val                 245 250 255 Arg Arg Gln Thr Ala Glu Ile Ala Asn Ser Tyr Phe Glu Gly Ala Asp             260 265 270 Phe Glu Lys Ile Ile Val Leu Pro Gly Leu Gly Asp Gln Ala Gly Met         275 280 285 Met Gly Ala Phe Ala Leu Ala Leu Ala Ala Glu Asn Lys     290 295 300 <210> 87 <211> 484 <212> PRT <213> Candida albicans <400> 87 Met Val His Leu Gly Pro Lys Pro Ala Gln Lys Arg Lys Gly Thr Phe   1 5 10 15 Thr Asp Val Ser Pro Gln Leu Leu Glu Ala Leu Lys Pro Ile Gln Glu              20 25 30 Gln Phe Thr Ile Ser Ala Asp Lys Leu Arg Ala Ile Val Lys His Phe          35 40 45 Ile Ser Glu Leu Asp Arg Gly Leu Ser Lys Ala Gly Gly Asn Ile Pro      50 55 60 Met Ile Pro Gly Trp Val Met Asp Phe Pro Thr Gly Lys Glu Thr Gly  65 70 75 80 Ser Tyr Leu Ala Ile Asp Leu Gly Gly Thr Asn Leu Arg Val Val Leu                  85 90 95 Val Lys Leu Gly Gly Asn Arg Asp Phe Asp Thr Thr Gln Ser Lys Phe             100 105 110 Ala Leu Pro Ala His Met Arg Thr Ala Thr Ser Asp Glu Leu Trp Asp         115 120 125 Phe Ile Ala Lys Cys Leu Lys Glu Phe Val Asp Glu Ile Tyr Pro Asp     130 135 140 Gly Cys Ser Glu Pro Leu Pro Leu Gly Phe Thr Phe Ser Tyr Pro Ala 145 150 155 160 Ser Gln Asn Arg Ile Asn Glu Gly Ile Leu Gln Arg Trp Thr Lys Gly                 165 170 175 Trp Ser Ile Asp Gly Ile Glu Gly Lys Asp Val Val Pro Met Leu Gln             180 185 190 Lys Ala Ile Lys Lys Val Gly Val Ile Asp Val Val Ala Leu Ile         195 200 205 Asn Asp Thr Thr Gly Thr Leu Val Ala Ser Met Tyr Thr Asp Pro Glu     210 215 220 Ala Lys Met Gly Leu Ile Phe Gly Thr Gly Val Asn Gly Ala Tyr Phe 225 230 235 240 Asp Val Val Lys Asp Ile Pro Lys Leu Glu Gly Lys Cys Pro Ser Asp                 245 250 255 Ile Pro Pro Glu Ser Pro Met Ala Ile Asn Cys Glu Tyr Gly Ser Phe             260 265 270 Asp Asn Glu Lys Tyr Ile Leu Pro Arg Thr Lys Tyr Asp Val Gln Ile         275 280 285 Asp Glu Glu Ser Pro Arg Gly Gln Gln Thr Phe Glu Lys Met Ile     290 295 300 Ser Gly Tyr Tyr Leu Gly Glu Val Leu Arg Leu Ile Leu Leu Glu Phe 305 310 315 320 Ala Glu Glu Lys Lys Leu Ile Phe Lys Gly Gln Asn Leu Asp Lys Leu                 325 330 335 Lys Val Pro Tyr Val Met Asp Ala Ser Tyr Pro Ser Lys Ile Glu Glu             340 345 350 Asp Pro Phe Glu Asn Leu Ser Asp Val Ala Asp Leu Phe Arg Glu Lys         355 360 365 Leu Gly Ile Glu Thr Thr Glu Pro Glu Arg Lys Ile Ile Arg Cys Leu     370 375 380 Ala Glu Leu Ile Gly Glu Arg Ser Ala Arg Phe Ser Val Cys Gly Ile 385 390 395 400 Ala Ala Ile Cys Gln Lys Arg Gly Tyr Lys Thr Ala His Cys Ala Ala                 405 410 415 Asp Gly Ser Val Tyr Asn Lys Tyr Pro Gly Phe Lys Glu Arg Thr Ala             420 425 430 Gln Ala Leu Arg Asp Ile Tyr Glu Trp Pro Ala Asp Val Lys Asp Pro         435 440 445 Ile Ile Ile Val Ala Glu Asp Gly Ser Gly Val Gly Ala Ala Val     450 455 460 Ile Ala Leu Thr Glu Lys Arg Leu Lys Glu Gly Lys Ser Val Gly 465 470 475 480 Leu Leu Gly Ala                 <210> 88 <211> 490 <212> PRT <213> Emericella nidulans <400> 88 Met Val Gly Val Gly Pro Lys Arg Pro Pro Ser Arg Lys Gly Ser Met   1 5 10 15 Ser Asp Val Pro Gln Asn Leu Leu Glu His Ile Lys His Phe Glu Glu              20 25 30 Ile Phe Thr Val Asp Thr Ala Thr Leu Lys Lys Ile Val Asp His Phe          35 40 45 Val Asn Glu Leu Thr Lys Gly Leu Ser Val Glu Gly Gly Asn Ile Pro      50 55 60 Met Asn Val Thr Trp Val Leu Gly Phe Pro Asp Gly Lys Glu Thr Gly  65 70 75 80 Thr Phe Leu Ala Leu Asp Met Gly Gly Thr Asn Leu Arg Val Cys Glu                  85 90 95 Ile Thr Leu Thr Glu Glu Lys Gly Gly Phe Asp Ile Ile Gln Ser Lys             100 105 110 Tyr Arg Met Pro Glu Glu Leu Lys Thr Gly Glu Glu Ala Glu Glu Leu Trp         115 120 125 Gln Tyr Ile Val Asp Cys Val Glu Gln Phe Ile Gln Phe His His Glu     130 135 140 Asn Glu Asn Leu Ser Lys Leu Pro Leu Gly Phe Thr Phe Ser Tyr Pro 145 150 155 160 Ala Thr Gln Asp Tyr Ile Asp His Gly Val Leu Gln Arg Trp Thr Lys                 165 170 175 Gly Phe Asp Ile Asp Gly Val Glu Gly Lys Asp Val Val Pro Pro Leu             180 185 190 Glu Lys Val Phe Lys Glu Arg Gly Leu Pro Ile Lys Val Ala Leu         195 200 205 Ile Asn Asp Thr Thr Gly Thr Leu Ile Ala Ser Ser Tyr Thr Asp Pro     210 215 220 Ala Met Lys Ile Gly Cys Ile Phe Gly Thr Gly Val Asn Ala Ala Tyr 225 230 235 240 Met Glu Asn Ala Gly Ser Ile Pro Lys Leu Ala His Met Asn Leu Pro                 245 250 255 Pro Asp Met Pro Ala Ile Asn Cys Glu Tyr Gly Ala Phe Asp Asn             260 265 270 Glu His Ile Val Leu Pro Leu Thr Lys Tyr Asp His Ile Ile Asp Arg         275 280 285 Asp Ser Pro Arg Pro Gly Gln Gln Ala Phe Glu Lys Met Thr Ala Gly     290 295 300 Leu Tyr Leu Gly Glu Ile Phe Arg Leu Ala Leu Val Asp Ile Leu Asp 305 310 315 320 Thr Gln Pro Gly Leu Ile Phe Lys Asp Gln Asp Thr Ser Gln Leu Arg                 325 330 335 Ile Pro Tyr Leu Leu Asp Ser Ser Phe Pro Ala Ala Ile Glu Glu Asp             340 345 350 Pro Tyr Glu Asn Leu Ile Glu Thr Ala Glu Leu Val Gln Asn Met Leu         355 360 365 Lys Ile Lys Ala Thr Arg Ser Glu Leu Glu Leu Met Arg Arg Leu Ala     370 375 380 Glu Leu Ile Gly Thr Arg Ala Ala Arg Leu Ser Ala Cys Gly Val Ala 385 390 395 400 Ala Ile Cys Lys Lys Lys Asn Ile Glu Ser Cys His Val Gly Ala Asp                 405 410 415 Gly Ser Val Phe Thr Lys Tyr Pro His Phe Lys Ala Arg Gly Ala Gln             420 425 430 Ala Leu Arg Glu Ile Leu Asp Trp Ala Pro Ser Glu Lys Asp Lys Val         435 440 445 Thr Ile His Ala Ala Glu Asp Gly     450 455 460 Ala Ala Leu Thr Leu Lys Arg Val Lys Ala Gly Asn Thr Ala Gly Ile 465 470 475 480 Arg Asp Ala Gln Ala Met Leu Ala Met Cys                 485 490 <210> 89 <211> 485 <212> PRT <213> Kluyveromyces lactis <400> 89 Met Val Arg Leu Gly Pro Lys Lys Pro Pro Ala Arg Lys Gly Ser Met   1 5 10 15 Ala Asp Val Ala Asn Leu Met Glu Gln Ile His Gly Leu Glu Thr              20 25 30 Leu Phe Thr Val Ser Ser Glu Lys Met Arg Ser Ile Val Lys His Phe          35 40 45 Ile Ser Glu Leu Asp Lys Gly Leu Ser Lys Lys Gly Gly Asn Ile Pro      50 55 60 Met Ile Pro Gly Trp Val Val Glu Tyr Pro Thr Gly Lys Glu Thr Gly  65 70 75 80 Asp Phe Leu Ala Leu Asp Leu Gly Gly Thr Asn Leu Arg Val Val Leu                  85 90 95 Val Lys Leu Gly Gly Asn His Asp Phe Asp Thr Thr Gln Asn Lys Tyr             100 105 110 Arg Leu Pro Asp His Leu Arg Thr Gly Thr Ser Glu Gln Leu Trp Ser         115 120 125 Phe Ile Ala Lys Cys Leu Lys Glu Phe Val Asp Glu Trp Tyr Pro Asp     130 135 140 Gly Val Ser Glu Pro Leu Pro Leu Gly Phe Thr Phe Ser Tyr Pro Ala 145 150 155 160 Ser Gln Lys Lys Ile Asn Ser Gly Val Leu Gln Arg Trp Thr Lys Gly                 165 170 175 Phe Asp Ile Glu Gly Val Glu Gly His Asp Val Val Pro Met Leu Gln             180 185 190 Glu Gln Ile Glu Lys Leu Asn Ile Pro Ile Asn Val Val Ala Leu Ile         195 200 205 Asn Asp Thr Thr Gly Thr Leu Val Ala Ser Leu Tyr Thr Asp Pro Gln     210 215 220 Thr Lys Met Gly Ile Ile Ile Gly Thr Gly Val Asn Gly Ala Tyr Tyr 225 230 235 240 Asp Val Val Ser Gly Ile Glu Lys Leu Glu Gly Leu Leu Pro Glu Asp                 245 250 255 Ile Gly Pro Asp Ser Pro Ala Ile Asn Cys Glu Tyr Gly Ser Phe             260 265 270 Asp Asn Glu His Leu Val Leu Pro Arg Thr Lys Tyr Asp Val Ile Ile         275 280 285 Asp Glu Glu Ser Pro Arg Gly Gln Gln Ala Phe Glu Lys Met Thr     290 295 300 Ser Gly Tyr Tyr Leu Gly Glu Ile Met Arg Leu Val Leu Leu Asp Leu 305 310 315 320 Tyr Asp Ser Gly Phe Ile Phe Lys Asp Gln Asp Ile Ser Lys Leu Lys                 325 330 335 Glu Ala Tyr Val Met Asp Thr Ser Tyr Pro Ser Lys Ile Glu Asp Asp             340 345 350 Pro Phe Glu Asn Leu Glu Asp Thr Asp Asp Leu Phe Lys Thr Asn Leu         355 360 365 Asn Ile Glu Thr Thr Val Val Glu Arg Lys Leu Ile Arg Lys Leu Ala     370 375 380 Glu Leu Val Gly Thr Arg Ala Ala Arg Leu Thr Val Cys Gly Val Ser 385 390 395 400 Ala Ile Cys Asp Lys Arg Gly Tyr Lys Thr Ala His Ile Ala Ala Asp                 405 410 415 Gly Ser Val Phe Asn Arg Tyr Pro Gly Tyr Lys Glu Lys Ala Ala Gln             420 425 430 Ala Leu Lys Asp Ile Tyr Asn Trp Asp Val Glu Lys Met Glu Asp His         435 440 445 Pro Ile Gln Leu Val Ala Ala Glu Asp Gly Ser Gly Val     450 455 460 Ile Ile Ala Cys Leu Thr Gln Lys Arg Leu Ala Ala Gly Lys Ser Val 465 470 475 480 Gly Ile Lys Gly Glu                 485 <210> 90 <211> 549 <212> PRT <213> Neosartora fumigata <400> 90 Met Thr Ala Pro Leu Val Lys Val Leu His Arg Met Gln Glu Leu Phe   1 5 10 15 Ser Asn Ile Val Ala Ala Leu Glu Ala Met Ile Val Phe Pro Ser Leu              20 25 30 Phe Arg Asp Pro Ser Arg Lys Arg Arg Lys Thr Phe His Gly Ala Tyr          35 40 45 Trp Arg Arg Arg Thr Leu Asp Asn Phe Ala Glu Glu Val Asp Arg Leu      50 55 60 Phe Thr Ser Pro Leu Ser Leu Arg Asn Met Ile Ile Met Ser Glu Lys  65 70 75 80 Ile Arg Glu Gln Phe Lys Ser Cys Leu Gln Ser Ser Pro Val Cys Met                  85 90 95 Leu Pro Ser Tyr Asn His Ala Leu Pro Ser Gly Thr Glu Lys Gly Thr             100 105 110 Tyr Leu Ala Leu Asp Val Gly Gly Ser Thr Phe Arg Val Ala Leu Ile         115 120 125 Glu Leu Gly Gly Ala Gly Ala Met Lys Ile Leu Gln Glu Ser Ser Ser     130 135 140 Pro Ile Asp Asn Asp Val Lys Leu Leu Glu Gly Thr Leu Phe Phe Asp 145 150 155 160 Trp Met Ala Glu Lys Ile Glu Ser Met Leu Ser Ala Val Gly Ala Asp                 165 170 175 Tyr Gly Arg Glu Ala Val Pro Leu Ser Met Gly Leu Ser Trp Ser Phe             180 185 190 Pro Ile Glu Gln Thr Ser Ile Ser Ser Gly Leu Val Ile His Met Gly         195 200 205 Lys Gly Phe Leu Cys Ser Asn Gly Thr Leu Gly Gln Glu Leu Gly Asp     210 215 220 Leu Ile Val Gln Ser Cys Arg Arg Arg Ser Leu Asn Val Arg Val Asp 225 230 235 240 Ala Ile Val Asn As Ser Ser Ala Ala Leu Ser Ser Ala Tyr Val                 245 250 255 Asp Pro Lys Thr Arg Met Ser Leu Ile Leu Gly Thr Gly Thr Asn Val             260 265 270 Ala Val His Phe Pro Val Arg Glu Ile Gly Leu Thr Lys Phe Gly Thr         275 280 285 Arg Pro Pro Gly Trp Phe Asp Tyr Ala Lys His Val Ile Ile Asn Ser     290 295 300 Glu Leu Ser Met Phe Gly Gly Gly Ile Leu Pro Met Thr Arg Trp Asp 305 310 315 320 Asp Ile Leu Asn Gln Thr His Leu Arg Pro Asp Tyr Gln Pro Leu Glu                 325 330 335 Tyr Met Val Thr Gly Arg Tyr Leu Gly Glu Ile Val Arg Leu Ile Ile             340 345 350 Thr Glu Ala Val Glu Thr Ala Asn Leu Phe Arg Gly Glu Leu Pro His         355 360 365 Ser Met Arg Asp Pro Tyr Ser Phe Asp Thr Ser Ile Val Ala Phe Leu     370 375 380 Glu Ala Asp Thr Ser Pro Ser Leu Val Ser Ser Ala Leu Leu Gln 385 390 395 400 Lys Glu His Thr Phe Pro Val Ser Ser Val Glu Asp Leu Arg Phe                 405 410 415 Leu Arg Arg Ile Cys Gln Ile Val Ser Lys Arg Ala Ala Gly Tyr Leu             420 425 430 Ala Thr Ala Ile His Ser Met Trp Cys Leu Arg Asn Asp Ala Glu Phe         435 440 445 Ser Asp Pro Thr Glu Ser Lys Ala Ser Ser Val Lys Asp Thr Gln Glu     450 455 460 Ile Thr Ile Val Glu Ser Glu Glu Asp Ser Ser Ser Ser Ser Ser Ile Ala 465 470 475 480 Cys Asp Gly Ser Val Ile Asn Lys Tyr Pro Gly Phe Arg Asp Arg Cys                 485 490 495 Gln Gly Tyr Leu Asn Gln Leu Thr Gln Gln Thr Asn Ser Ser Gln Gly             500 505 510 Pro Leu Asp Pro Ala Thr Ser Pro Tyr Ile Arg Leu Glu Leu Ala Pro         515 520 525 Glu Ser Ala Ile Leu Gly Ala Ala Val Ala Val Ala Val Ala Val Glu     530 535 540 Asp Gln Lys Ser Gly 545 <210> 91 <211> 485 <212> PRT <213> Saccharomyces cerevisiae <400> 91 Met Val His Leu Gly Pro Lys Lys Pro Gln Ala Arg Lys Gly Ser Met   1 5 10 15 Ala Asp Val Pro Lys Glu Leu Met Asp Glu Ile His Gln Leu Glu Asp              20 25 30 Met Phe Thr Val Asp Ser Glu Thr Leu Arg Lys Val Val Lys His Phe          35 40 45 Ile Asp Glu Leu Asn Lys Gly Leu Thr Lys Lys Gly Gly Asn Ile Pro      50 55 60 Met Ile Pro Gly Trp Val Met Glu Phe Pro Thr Gly Lys Glu Ser Gly  65 70 75 80 Asn Tyr Leu Ala Ile Asp Leu Gly Gly Thr Asn Leu Arg Val Val Leu                  85 90 95 Val Lys Leu Ser Gly Asn His Thr Phe Asp Thr Thr Gln Ser Lys Tyr             100 105 110 Lys Leu Pro His Asp Met Arg Thr Thr Lys His Gln Glu Glu Leu Trp         115 120 125 Ser Phe Ile Ala Asp Ser Leu Lys Asp Phe Met Val Glu Gln Glu Leu     130 135 140 Leu Asn Thr Lys Asp Thr Leu Pro Leu Gly Phe Thr Phe Ser Tyr Pro 145 150 155 160 Ala Ser Gln Asn Lys Ile Asn Glu Gly Ile Leu Gln Arg Trp Thr Lys                 165 170 175 Gly Phe Asp Ile Pro Asn Val Glu Gly His Asp Val Val Pro Leu Leu             180 185 190 Gln Asn Glu Ile Ser Lys Arg Glu Leu Pro Ile Glu Ile Val Ala Leu         195 200 205 Ile Asn Asp Thr Val Gly Thr Leu Ile Ala Ser Tyr Tyr Thr Asp Pro     210 215 220 Glu Thr Lys Met Gly Val Ile Phe Gly Thr Gly Val Asn Gly Ala Phe 225 230 235 240 Tyr Asp Val Val Ser Asp Ile Glu Lys Leu Glu Gly Lys Leu Ala Asp                 245 250 255 Asp Ile Pro Ser Asn Ser Pro Met Ala Ile Asn Cys Glu Tyr Gly Ser             260 265 270 Phe Asp Asn Glu His Leu Val Leu Pro Arg Thr Lys Tyr Asp Val Ala         275 280 285 Val Asp Glu Gln Ser Pro Arg Pro Gly Gln Gln Ala Phe Glu Lys Met     290 295 300 Thr Ser Gly Tyr Tyr Leu Gly Glu Leu Leu Arg Leu Val Leu Leu Glu 305 310 315 320 Leu Asn Glu Lys Gly Leu Met Leu Lys Asp Gln Asp Leu Ser Lys Leu                 325 330 335 Lys Gln Pro Tyr Ile Met Asp Thr Ser Tyr Pro Ala Arg Ile Glu Asp             340 345 350 Asp Pro Phe Glu Asn Leu Glu Asp Thr Asp Asp Ile Phe Gln Lys Asp         355 360 365 Phe Gly Val Lys Thr Thr Leu Pro Glu Arg Lys Leu Ile Arg Arg Leu     370 375 380 Cys Glu Leu Ile Gly Thr Arg Ala Ala Arg Leu Ala Val Cys Gly Ile 385 390 395 400 Ala Ala Ile Cys Gln Lys Arg Gly Tyr Lys Thr Gly His Ile Ala Ala                 405 410 415 Asp Gly Ser Val Tyr Asn Lys Tyr Pro Gly Phe Lys Glu Ala Ala Ala             420 425 430 Lys Gly Leu Arg Asp Ile Tyr Gly Trp Thr Gly Asp Ala Ser Lys Asp         435 440 445 Pro Ile Thr Ile Val Pro Ala Glu Asp Gly Ser Gly Ala Gly Ala Ala     450 455 460 Val Ile Ala Leu Ser Glu Lys Arg Ile Ala Glu Gly Lys Ser Leu 465 470 475 480 Gly Ile Ile Gly Ala                 485 <210> 92 <211> 484 <212> PRT <213> Schizosaccharomyces pombe <400> 92 Met Ser Leu His Asp Ala Tyr His Trp Pro Ser Arg Thr Pro Ser Arg   1 5 10 15 Lys Gly Ser Asn Ile Lys Leu Asn Lys Thr Leu Gln Asp His Leu Asp              20 25 30 Glu Leu Glu Glu Gln Phe Thr Ile Pro Thr Glu Leu Leu His Arg Val          35 40 45 Thr Asp Arg Phe Val Ser Glu Leu Tyr Lys Gly Leu Thr Thr Asn Pro      50 55 60 Gly Asp Val Pro Met Val Pro Thr Trp Ile Ile Gly Thr Pro Asp Gly  65 70 75 80 Asn Glu His Gly Ser Tyr Leu Ala Leu Asp Leu Gly Gly Thr Asn Leu                  85 90 95 Arg Val Cys Ala Val Glu Val Gln Gly Asn Gly Lys Phe Asp Ile Thr             100 105 110 Gln Ser Lys Tyr Arg Leu Pro Gln Glu Leu Lys Val Gly Thr Arg Glu         115 120 125 Ala Leu Phe Asp Tyr Ile Ala Asp Cys Ile Lys Lys Phe Val Glu Glu     130 135 140 Val His Pro Gly Lys Ser Gln Asn Leu Glu Ile Gly Phe Thr Phe Ser 145 150 155 160 Tyr Pro Cys Val Gln Arg Ser Ile Asn Asp Ala Ser Leu Val Ala Trp                 165 170 175 Thr Lys Gly Phe Asp Ile Asp Gly Val Glu Gly Glu Ser Val Gly Pro             180 185 190 Leu Leu Ser Ala Ala Leu Lys Arg Val Gly Cys Asn Asn Val Arg Leu         195 200 205 Asn Ile Leu Ser Asp Thr Thr Gly Thr Leu Val Ala Ser Asn Tyr     210 215 220 Ala Ser Pro Gly Thr Gly Ily Gly Val Ile Phe Gly Thr Gly Cys Asn 225 230 235 240 Ala Cys Tyr Ile Glu Lys Phe Ser Glu Ile Pro Lys Leu His Lys Tyr                 245 250 255 Asp Phe Pro Glu Asp Met Asn Met Ile Ile Asn Cys Glu Trp Cys Asp             260 265 270 Phe Asp Asn Gln His Val Val Leu Pro Arg Thr Lys Tyr Asp Val Ala         275 280 285 Ile Asp Glu Glu Ser Pro Arg Gly Leu Gln Thr Tyr Glu Lys Met     290 295 300 Ile Ala Gly Cys Tyr Leu Gly Asp Ile Leu Arg Ile Leu Leu Asp 305 310 315 320 Leu Tyr Glu Gln Gly Ala Leu Phe Asn Gly Gln Asp Val Thr Lys Ile                 325 330 335 Arg Asp Pro Leu Ala Met Asp Thr Ser Val Leu Ser Ala Ile Glu Val             340 345 350 Asp Pro Phe Glu Asn Leu Asp Glu Thr Gln Thr Leu Phe Glu Glu Thr         355 360 365 Tyr Gly Leu Lys Thr Thr Glu Glu Glu Arg Gln Phe Ile Arg Arg Ala     370 375 380 Cys Glu Leu Ile Gly Thr Arg Ser Ala Arg Leu Ser Ala Cys Gly Val 385 390 395 400 Cys Ala Leu Val Arg Lys Met Asn Lys Pro Ser Met Ile Val Gly Thr                 405 410 415 Asp Gly Ser Val Tyr Asn Leu Tyr Pro Arg Phe Lys Asp Arg Leu Ala             420 425 430 Gln Ala Phe Lys Asp Ile Leu Gly Glu Glu Ile Gly Ser Lys Val Val         435 440 445 Thr Ile Pro Ala Glu Asp Gly Ser Gly Val Gly Ala Ala Leu Val Ser     450 455 460 Ala Leu Glu Ala Lys Gly Lys Ala Leu Thr Ser Asp Ile Leu Ala Glu 465 470 475 480 His Leu Lys Asn                 <210> 93 <211> 478 <212> PRT <213> Schwanniomyces occidentalis <400> 93 Met Val His Leu Gly Pro Lys Pro Pro Gln His Arg Lys Gly Ser Phe   1 5 10 15 Leu Asp Val Pro Glu Tyr Leu Leu Lys Glu Leu Thr Glu Leu Glu Gly              20 25 30 Leu Leu Thr Val Ser Gly Glu Thr Leu Arg Lys Ile Thr Asp His Phe          35 40 45 Ile Ser Glu Leu Glu Lys Gly Leu Ser Lys Gln Gly Gly Asn Ile Pro      50 55 60 Met Ile Pro Gly Trp Val Met Asp Phe Pro Thr Gly Lys Glu Met Gly  65 70 75 80 Asp Tyr Leu Ala Ile Asp Leu Gly Gly Thr Asn Leu Arg Val Val Leu                  85 90 95 Val Lys Leu Gly Gly Asn Arg Asp Phe Asp Thr Thr Gln Ser Lys Phe             100 105 110 Ala Leu Pro Glu Asn Met Arg Thr Ala Lys Ser Glu Glu Leu Trp Glu         115 120 125 Phe Ile Ala Glu Cys Leu Gln Lys Phe Val Glu Glu Glu Phe Arg Asn     130 135 140 Gly Val Leu Ser Asn Leu Pro Leu Gly Phe Thr Phe Ser Tyr Pro Ala 145 150 155 160 Ser Gln Gly Ser Ile Asn Glu Gly Tyr Leu Gln Arg Trp Thr Lys Gly                 165 170 175 Phe Asp Ile Glu Gly Val Glu Gly His Asp Val Val Pro Met Leu Gln             180 185 190 Ala Ala Ile Glu Lys Arg Lys Val Ile Glu Val Val Ala Leu Ile         195 200 205 Asn Asp Thr Thr Gly Thr Leu Val Ala Ser Met Tyr Thr Asp Pro Glu     210 215 220 Ala Lys Met Gly Leu Phe Ser Gly Thr Gly Cys Asn Gly Ala Tyr Tyr 225 230 235 240 Asp Val Val Asp Asn Ile Pro Lys Leu Glu Gly Lys Val Pro Asp Asp                 245 250 255 Ile Lys Ser Ser Ser Pro Ale Ile Asn Cys Glu Tyr Gly Ala Phe             260 265 270 Asp Asn Glu His Ile Ile Leu Pro Arg Thr Lys Tyr Asp Ile Gln Ile         275 280 285 Asp Glu Glu Ser Pro Arg Pro Gly Gln Gln Ala Phe Glu Lys Met Ile     290 295 300 Ser Gly Tyr Tyr Leu Gly Glu Val Leu Arg Leu Ile Leu Leu Asp Leu 305 310 315 320 Thr Ser Lys Gln Leu Ile Phe Lys Asp Gln Asp Leu Ser Lys Leu Gln                 325 330 335 Val Pro Phe Ile Leu Asp Thr Ser Ile Pro Ala Arg Ile Glu Glu Asp             340 345 350 Pro Phe Glu Asn Leu Ser Asp Val Glu Glu Leu Phe Gln Glu Ile Leu         355 360 365 Gly Ile Gln Thr Thr Ser Pro Glu Arg Lys Ile Ile Arg Arg Leu Ala     370 375 380 Glu Leu Ile Gly Glu Arg Ser Ala Arg Leu Ser Ile Cys Gly Ile Ala 385 390 395 400 Ala Ile Cys Lys Lys Arg Gly Tyr Lys Thr Ala His Cys Ala Ala Asp                 405 410 415 Gly Ser Val Tyr Asn Lys Tyr Pro Gly Phe Lys Glu Arg Ala Ala Lys             420 425 430 Gly Leu Arg Asp Ile Phe Gln Trp Glu Ser Glu Glu Asp Pro Ile Val         435 440 445 Ile Val Pro Ala Glu Asp Gly Leu Gly Ala Gla Ala Ala Ile Ile Ala     450 455 460 Ala Leu Thr Glu Lys Arg Leu Lys Asp Gly Leu Pro Leu Val 465 470 475

Claims (15)

알카니보락스(Alcanivorax), 바실러스(Bacillus), 비피도박테리움(Bifidobacterium), 코리네박테리움(Corynebacterium), 엔테로코커스(Enterococcus), 지오바실러스(Geobacillus), 락토바실러스(Lactobacillus), 마리노박터(Marinobacter), 메틸로바실러스(Methylobacillus), 리조비움(Rhizobium), 스태필로코커스(Staphylococcus), 스트렙토코커스(Streptococcus) 및 스트렙토마이세스(Streptomyces) 속 미생물로 이루어진 군으로부터 선택되는 미생물 유래의 프럭토스 1,6-비스포스페이트 알돌레이즈 효소를 유효성분으로 함유하는 프럭토스(fructose)에서 타가토스(tagatose)를 생산하기 위한 조성물로서,
상기 바실러스는 바실러스 알칼로필루스(Bacillus alcalophilus), 바실러스 아밀로리쿼파시엔스(Bacillus amyloliquefaciens), 바실러스 아조토포르만스(Bacillus azotoformans), 바실러스 셀룰로실리티쿠스(Bacillus cellulosilyticus), 바실러스 코아귤란스(Bacillus coagulans), 바실러스 사이토톡시쿠스(Bacillus cytotoxicus), 바실러스 할로두란스(Bacillus halodurans), 바실러스 리케니포르미스(Bacillus licheniformis), 바실러스 마카우엔시스(Bacillus macauensis), 바실러스 메가테리움(Bacillus megaterium), 바실러스 메타놀리쿠스(Bacillus methanolicus), 바실러스 닐소니(Bacillus nealsonii), 바실러스 슈도피르무스(Bacillus pseudofirmus), 바실러스 셀리니티레두센스(Bacillus selenitireducens) 및 바실러스 바이헨스테파넨시스(Bacillus weihenstephanensis)로 이루어진 군으로부터 선택되는 어느 하나의 미생물인 것을 특징으로 하는 조성물.Al Carney borax (Alcanivorax), Bacillus (Bacillus), Bifidobacterium (Bifidobacterium), Corynebacterium (Corynebacterium), Enterococcus (Enterococcus), geo Bacillus (Geobacillus), Lactobacillus bacteria (Lactobacillus), Marino bakteo ( Marinobacter), the Bacillus (Methylobacillus), separation tank emptying (Rhizobium), Staphylococcus (Staphylococcus), Streptococcus (Streptococcus) and fructose derived from a microorganism selected from the group consisting of Streptomyces (Streptomyces) spp methyl 1, A composition for producing tagatose from fructose containing 6-bisphosphate aldolase enzyme as an active ingredient,
The Bacillus is Bacillus al knife peel Ruth (Bacillus alcalophilus), Bacillus amyl Lowry query Pacific Enschede (Bacillus amyloliquefaciens), Bacillus azo Sat formate only switch (Bacillus azotoformans), Bacillus cellulite in silico T kusu (Bacillus cellulosilyticus), Bacillus core tangerine Fragrance Such as Bacillus coagulans , Bacillus cytotoxicus , Bacillus halodurans , Bacillus licheniformis , Bacillus macauensis , Bacillus megaterium , From the group consisting of Bacillus methanolicus , Bacillus nealsonii , Bacillus pseudofirmus , Bacillus selenitireducens , and Bacillus weihenstephanensis , from the group consisting of Bacillus methanolicus , Bacillus nealsonii , Bacillus pseudofirmus , Bacillus selenitireducens and Bacillus weihenstephanensis One of the microorganisms selected The composition according to claim. 제1항에 있어서, 헥소카이네이즈 또는 프럭토카이네이즈; 및 타가토스-6-포스페이트 피타아제;로 이루어진 군으로부터 선택되는 하나 이상의 효소를 추가로 포함하는 것을 특징으로 하는 프럭토스(fructose)에서 타가토스(tagatose)를 생산하기 위한 조성물.3. The composition of claim 1, wherein the composition comprises hexokinase or fructokinase; And a tagatose-6-phosphate phytase. The composition for producing tagatose in a fructose according to claim 1, 제2항에 있어서, 상기 타가토스-6-포스페이트 피타아제 대신에 타가토스-6-포스파타아제 효소를 추가로 포함하는 것을 특징으로 하는 프럭토스(fructose)에서 타가토스(tagatose)를 생산하기 위한 조성물.3. The method according to claim 2, further comprising a tagatose-6-phosphatase enzyme in place of the tagatose-6-phosphate tagase in order to produce tagatose from fructose. Composition. 제1항 내지 제3항 중 어느 한 항에 있어서, 상기 효소들은 고정화된 효소인 것을 특징으로 하는 프럭토스(fructose)에서 타가토스(tagatose)를 생산하기 위한 조성물.The composition for producing tagatose in fructose according to any one of claims 1 to 3, wherein the enzymes are immobilized enzymes. 제4항에 있어서, 상기 고정화 방법은 흡착법, 공유결합법 또는 가두기법(entrapment)인 것을 특징으로 하는 프럭토스(fructose)에서 타가토스(tagatose)를 생산하기 위한 조성물.5. The composition according to claim 4, wherein the immobilization method is an adsorption method, a covalent bonding method, or a entrapment method. 제1항에 있어서, 상기 프럭토스 1,6-비스포스페이트 알돌레이즈 효소는 알카니보락스 보르쿠멘시스(Alcanivorax borkumensis), 바실러스 알칼로필루스(Bacillus alcalophilus), 바실러스 아밀로리쿼파시엔스(Bacillus amyloliquefaciens), 바실러스 아조토포르만스(Bacillus azotoformans), 바실러스 셀룰로실리티쿠스(Bacillus cellulosilyticus), 바실러스 코아귤란스(Bacillus coagulans), 바실러스 사이토톡시쿠스(Bacillus cytotoxicus), 바실러스 할로두란스(Bacillus halodurans), 바실러스 리케니포르미스(Bacillus licheniformis), 바실러스 마카우엔시스(Bacillus macauensis), 바실러스 메가테리움(Bacillus megaterium), 바실러스 메타놀리쿠스(Bacillus methanolicus), 바실러스 닐소니(Bacillus nealsonii), 바실러스 슈도피르무스(Bacillus pseudofirmus), 바실러스 셀리니티레두센스(Bacillus selenitireducens), 바실러스 바이헨스테파넨시스(Bacillus weihenstephanensis), 비피도박테리움 브레베(Bifidobacterium breve), 코리네박테리움 글루타미쿰(Corynebacterium glutamicum), 코리네박테리움 크로펜스테티(Corynebacterium kroppenstedtii), 엔테로코커스 아시니(Enterococcus asini), 엔테로코커스 카캐(Enterococcus caccae), 엔테로코커스 카셀리플라부스(Enterococcus casseliflavus), 엔테로코커스 세코룸(Enterococcus cecorum), 엔테로코커스 콜룸배(Enterococcus columbae), 엔테로코커스 패칼리스(Enterococcus faecalis), 엔테로코커스 패시움(Enterococcus faecium), 엔테로코커스 플라베센스(Enterococcus flavescens), 엔테로코커스 길부스(Enterococcus gilvus), 엔테로코커스 해모페록시두스(Enterococcus haemoperoxidus), 엔테로코커스 이탈리쿠스(Enterococcus italicus), 엔테로코커스 모라비엔시스(Enterococcus moraviensis), 엔테로코커스 문티(Enterococcus mundtii), 엔테로코커스 팔렌스(Enterococcus pallens), 엔테로코커스 페니쿨리콜라(Enterococcus phoeniculicola), 엔테로코커스 라피노수스(Enterococcus raffinosus), 엔테로코커스 사카로리티쿠스(Enterococcus saccharolyticus), 엔테로코커스 종(Enterococcus sp .), 엔테로코커스 빌로룸(Enterococcus villorum), 지오바실러스 카우스토필루스(Geobacillus kaustophilus), 지오바실러스 종(Geobacillus sp.), 지오바실러스 스테아로써모필루스(Geobacillus stearothermophilus), 지오바실러스 써모데니트리피칸스(Geobacillus thermodenitrificans), 락토바실러스 애시도필루스(Lactobacillus acidophilus), 락토바실러스 아밀로리티쿠스(Lactobacillus amylolyticus), 락토바실러스 안트리(Lactobacillus antri), 락토바실러스 카제이(Lactobacillus casei), 락토바실러스 크리스파투스(Lactobacillus crispatus), 락토바실러스 커바투스(Lactobacillus curvatus), 락토바실러스 델브루에키 아종 불가르쿠스(Lactobacillus delbrueckii subsp . bulgaricus), 락토바실러스 헬베티쿠스(Lactobacillus helveticus), 락토바실러스 이너스(Lactobacillus iners), 락토바실러스 존슨아이(Lactobacillus johnsonii), 락토바실러스 뮤리누스(Lactobacillus murinus), 락토바실러스 파라카제이 아종 파라카제이(Lactobacillus paracasei subsp . paracasei), 락토바실러스 플란타룸 아종 플란타룸(Lactobacillus plantarum subsp . plantarum), 락토바실러스 루테리(Lactobacillus reuteri), 락토바실러스 람노수스(Lactobacillus rhamnosus), 락토바실러스 루미니스(Lactobacillus ruminis), 락토바실러스 살리바리우스(Lactobacillus salivarius), 락토바실러스 종(Lactobacillus sp .), 마리노박터 히드로카보노클라스티쿠스(Marinobacter hydrocarbonoclasticus), 메틸로바실러스 플라젤라투스(Methylobacillus flagellatus), 리조비움 그라하미(Rhizobium grahamii), 리조비움 레규미노사룸 비브이. 트리폴리아이(Rhizobium leguminosarum bv . trifolii), 리조비움 루피니(Rhizobium lupini), 리조비움 종(Rhizobium sp .), 스태필로코커스 에피더미디스(Staphylococcus epidermidis), 스트렙토코커스 써모필러스(Streptococcus thermophilus), 스트렙토마이세스 아베미틸리스(Streptomyces avermitilis) 및 스트렙토마이세스 비리도크로모게네스(Streptomyces viridochromogenes)로 이루어진 군으로부터 선택되는 미생물로부터 유래한 효소인 것을 특징으로 하는 프럭토스(fructose)에서 타가토스(tagatose)를 생산하기 위한 조성물.The method according to claim 1, wherein the fructose 1,6-bisphosphate aldolase enzyme is selected from the group consisting of Alcanivorax borkumensis), Bacillus know Phil sword Luce (Bacillus alcalophilus), Bacillus amyl Lori query Pacifico Enschede (Bacillus amyloliquefaciens), Bacillus azo only Saturday's Fort (Bacillus azotoformans), silica as Bacillus cellulose tea kusu (Bacillus cellulosilyticus), Bacillus lance core tangerine (Bacillus coagulans), Bacillus Saito ethoxy kusu (Bacillus cytotoxicus), Bacillus halo two lance (Bacillus halodurans), Bacillus Fort Lee Kenny Miss (Bacillus licheniformis), Macau N-Sys Bacillus (Bacillus macauensis), MEGATHERIUM Bacillus (Bacillus megaterium), Bacillus meta fun Syracuse (Bacillus methanolicus), Bacillus carbonyl Sony (Bacillus nealsonii , Bacillus pseudofirmus), Bacillus selrini Tire, two sense (Bacillus selenitireducens), Bacillus by Henderson seutepa cis norbornene (Bacillus weihenstephanensis), Bifidobacterium breve (Bifidobacterium breve), Corynebacterium glutamicum (Corynebacterium glutamicum ), Corynebacterium kroppenstedtii , Enterococcus asini ), enterococcus cata ( Enterococcus caccae), Enterococcus Kassel ripple La Booth (Enterococcus casseliflavus), Enterococcus three Corum (Enterococcus cecorum ), enterococcus colomba ( Enterococcus columbae ), Enterococcus faecalis faecalis ), Enterococcus sp. faecium , enterococcus flavosense flavescens , Enterococcus gilvus ), enterococcus halobacterium ( Enterococcus haemoperoxidus , Enterococcus italicus , Enterococcus moraviensis , Enterococcus, mundtii ), Enterococcus pallens), Enterococcus penny Cooley coke (Enterococcus phoeniculicola), Enterococcus raffinose Versus (Enterococcus raffinosus ), enterococcus saccharolyticus ( Enterococcus saccharolyticus), Enterococcus species (Enterococcus sp . ), Enterococcus villorum , Geobacillus kaustophilus), geo Bacillus species (Geobacillus sp.), loose a brush (Geobacillus as geo Bacillus stearate stearothermophilus ), Geobacillus thermodenitrificans ( Geobacillus thermodenitrificans ), Lactobacillus acidophilus ( Lactobacillus acidophilus , Lactobacillus amylolyticus amylolyticus , Lactobacillus antri , Lactobacillus &lt; RTI ID = 0.0 &gt; casei ), Lactobacillus crispatus , Lactobacillus &lt; RTI ID = 0.0 &gt; gt; Lactobacillus &lt; / RTI &gt; delbrueckii subsp . bulgaricus), Lactobacillus helveticus (Lactobacillus helveticus , Lactobacillus &lt; RTI ID = 0.0 &gt; iners , Lactobacillus johnsonii ), Lactobacillus murinus ), Lactobacillus paracasei paracasei ( Lactobacillus paracasei subsp . paracasei , Lactobacillus plantarum, Lactobacillus plantarum subsp . plantarum , Lactobacillus reuteri , Lactobacillus rhamnosus , Lactobacillus ruminis), Lactobacillus salivarius (Lactobacillus salivarius , Lactobacillus sp . ), Marinobacter ( Marinobacter &lt; RTI ID = 0.0 &gt; hydrocarbonoclasticus), Bacillus methyl Plastic gelatinase tooth (Methylobacillus flagellatus , Rhizobium grahamii , Rhizobium reguminosa , and so on. Rhizobium leguminosarum bv . Trifolii , Rhizobium &lt; RTI ID = 0.0 &gt; lupini , Rhizobium sp . ), Staphylococcus epidermidis (Staphylococcus epidermidis), Streptococcus Thermo filler's (Streptococcus thermophilus), Streptomyces Abbe US subtilis (Streptomyces wherein the enzyme is an enzyme derived from a microorganism selected from the group consisting of avermitilis , Streptomyces viridochromogenes , and a fructose. 제6항에 있어서, 상기 프럭토스 1,6-비스포스페이트 알돌레이즈 효소는 서열번호 1 내지 38 및 서열번호 42 내지 72의 아미노산 서열로 이루어진 군으로부터 선택되는 어느 하나의 아미노산 서열을 갖는 것을 특징으로 하는 프럭토스(fructose)에서 타가토스(tagatose)를 생산하기 위한 조성물.The fructose 1,6-bisphosphate aldolase enzyme according to claim 6, wherein the fructose 1,6-bisphosphate aldolase enzyme has an amino acid sequence selected from the group consisting of the amino acid sequences of SEQ ID NOS: 1 to 38 and SEQ ID NOS: 42 to 72 A composition for producing tagatose in fructose. 제2항에 있어서, 상기 프럭토카이네이즈는 바실러스 서브틸리스(Bacillus subtilis), 에세리키아 콜라이(Escherichia coli), 푸소박테리움 모르티페룸(Fusobacterium mortiferum), 클렙시엘라 뉴모니애(Klebsiella pneumoniae), 락토코커스 락티스(Lactococcus lactis), 페디오코커스 펜토사세우스(Pediococcus pentosaceus), 리조비움 레규미노사룸(Rhizobium leguminosarum), 살모넬라 톰슨(Salmonella thompson), 살모넬라 티피무리움(Salmonella typhimurium), 스트렙토코커스 뮤탄스(Streptococcus mutans), 비브리오 알지노리티쿠스(Vibrio alginolyticus) 및 자이모모나스 모빌리스(Zymomonas mobilis)로 이루어진 군으로부터 선택되는 미생물로부터 유래한 효소인 것을 특징으로 하는 프럭토스(fructose)에서 타가토스(tagatose)를 생산하기 위한 조성물.The method of claim 2, wherein the fructo kinase is Bacillus subtilis (Bacillus subtilis), Escherichia coli (Escherichia coli , Fusobacterium &lt; RTI ID = 0.0 &gt; mortiferum , Klebsiella pneumoniae , Lactococcus lactis , Pediococcus pentosaceus , Rhizobium &lt; RTI ID = 0.0 &gt; leguminosarum), Salmonella Thompson (Salmonella thompson), Salmonella typhimurium (Salmonella typhimurium , Streptococcus mutans, mutans , Vibrio alginolyticus and Zymomonas &lt; RTI ID = 0.0 &gt; mobilis) composition for the production of tagatose (tagatose) in fructose (fructose), characterized in that the enzyme derived from a microorganism selected from the group consisting of. 제8항에 있어서, 상기 프럭토카이네이즈는 서열번호 73 내지 86의 아미노산 서열로 이루어진 군으로부터 선택되는 어느 하나의 아미노산 서열을 갖는 것을 특징으로 하는 프럭토스(fructose)에서 타가토스(tagatose)를 생산하기 위한 조성물.9. The method according to claim 8, wherein the fructokinase has an amino acid sequence selected from the group consisting of the amino acid sequences of SEQ ID NOs: 73 to 86. The fructose- / RTI &gt; 제2항에 있어서, 상기 헥소카이네이즈는 캔디다 알비칸스(Candida albicans), 에메리셀라 니둘란스(Emericella nidulans), 클루이베로마이세스 락티스(Kluyveromyces lactis), 네오사르토리아 푸미가타(Neosartorya fumigata), 사카로마이세스 세레비지애(Saccharomyces cerevisiae), 스키조사카로마이세스 폼베(Schizosaccharomyces pombe) 및 쉬바니오마이세스 옥시덴탈리스(Schwanniomyces occidentalis)로 이루어진 군으로부터 선택되는 미생물로부터 유래한 효소인 것을 특징으로 하는 프럭토스(fructose)에서 타가토스(tagatose)를 생산하기 위한 조성물.3. The method according to claim 2, wherein the hexokinase is selected from the group consisting of Candida albicans , Emericella &lt; RTI ID = 0.0 &gt; nidulans), Cluj Vero Mai Seth lactis (Kluyveromyces lactis , Neosartorya &lt; RTI ID = 0.0 &gt; fumigata), Celebi as Saccharomyces My jiae access (Saccharomyces S. cerevisiae ), skiing investigation, Schizosaccharomyces wherein the enzyme is an enzyme derived from a microorganism selected from the group consisting of pombe , Schwanniomyces occidentalis , and fructose. 제10항에 있어서, 상기 헥소카이네이즈는 서열번호 87 내지 93의 아미노산 서열로 이루어진 군으로부터 선택되는 어느 하나의 아미노산 서열을 갖는 것을 특징으로 하는 프럭토스(fructose)에서 타가토스(tagatose)를 생산하기 위한 조성물.[Claim 11] The method according to claim 10, wherein the hexokinase has an amino acid sequence selected from the group consisting of the amino acid sequences of SEQ ID NOs: 87 to 93. The method for producing tagatose from fructose Composition. 프럭토스(fructose)에 헥소카이네이즈 또는 프럭토카이네이즈; 알카니보락스(Alcanivorax), 바실러스(Bacillus), 비피도박테리움(Bifidobacterium), 코리네박테리움(Corynebacterium), 엔테로코커스(Enterococcus), 지오바실러스(Geobacillus), 락토바실러스(Lactobacillus), 마리노박터(Marinobacter), 메틸로바실러스(Methylobacillus), 리조비움(Rhizobium), 스태필로코커스(Staphylococcus), 스트렙토코커스(Streptococcus) 및 스트렙토마이세스(Streptomyces) 속 미생물로 이루어진 군으로부터 선택되는 미생물 유래의 프럭토스 1,6-비스포스페이트 알돌레이즈; 및 타가토스-6-포스페이트 피타아제;를 첨가하여 반응시키는 단계를 포함하는 프럭토스(fructose)에서 타가토스(tagatose)를 생산하는 방법으로서,
상기 바실러스는 바실러스 알칼로필루스(Bacillus alcalophilus), 바실러스 아밀로리쿼파시엔스(Bacillus amyloliquefaciens), 바실러스 아조토포르만스(Bacillus azotoformans), 바실러스 셀룰로실리티쿠스(Bacillus cellulosilyticus), 바실러스 코아귤란스(Bacillus coagulans), 바실러스 사이토톡시쿠스(Bacillus cytotoxicus), 바실러스 할로두란스(Bacillus halodurans), 바실러스 리케니포르미스(Bacillus licheniformis), 바실러스 마카우엔시스(Bacillus macauensis), 바실러스 메가테리움(Bacillus megaterium), 바실러스 메타놀리쿠스(Bacillus methanolicus), 바실러스 닐소니(Bacillus nealsonii), 바실러스 슈도피르무스(Bacillus pseudofirmus), 바실러스 셀리니티레두센스(Bacillus selenitireducens) 및 바실러스 바이헨스테파넨시스(Bacillus weihenstephanensis)로 이루어진 군으로부터 선택되는 어느 하나의 미생물인 것을 특징으로 하는 프럭토스(fructose)에서 타가토스(tagatose)를 생산하는 방법.Hexokinase or fructokinase in fructose; Al Carney borax (Alcanivorax), Bacillus (Bacillus), Bifidobacterium (Bifidobacterium), Corynebacterium (Corynebacterium), Enterococcus (Enterococcus), geo Bacillus (Geobacillus), Lactobacillus bacteria (Lactobacillus), Marino bakteo ( Marinobacter), the Bacillus (Methylobacillus), separation tank emptying (Rhizobium), Staphylococcus (Staphylococcus), Streptococcus (Streptococcus) and fructose derived from a microorganism selected from the group consisting of Streptomyces (Streptomyces) spp methyl 1, 6-bisphosphate aldolase; And tagatose-6-phosphate phytase; and a step of reacting the fructose with the tagatose-
The Bacillus is Bacillus al knife peel Ruth (Bacillus alcalophilus), Bacillus amyl Lowry query Pacific Enschede (Bacillus amyloliquefaciens), Bacillus azo Sat formate only switch (Bacillus azotoformans), Bacillus cellulite in silico T kusu (Bacillus cellulosilyticus), Bacillus core tangerine Fragrance Such as Bacillus coagulans , Bacillus cytotoxicus , Bacillus halodurans , Bacillus licheniformis , Bacillus macauensis , Bacillus megaterium , From the group consisting of Bacillus methanolicus , Bacillus nealsonii , Bacillus pseudofirmus , Bacillus selenitireducens , and Bacillus weihenstephanensis , from the group consisting of Bacillus methanolicus , Bacillus nealsonii , Bacillus pseudofirmus , Bacillus selenitireducens and Bacillus weihenstephanensis One of the microorganisms selected Heterotrophic methods for producing monohydrate (tagatose) in the fructose (fructose), characterized. 제12항에 있어서, 상기 효소들은 고정화된 효소인 것을 특징으로 하는 프럭토스(fructose)에서 타가토스(tagatose)를 생산하는 방법.13. The method according to claim 12, wherein the enzymes are immobilized enzymes. 10. A method for producing tagatose from fructose. 제12항에 있어서, 상기 효소 반응은 세포 내에서 수행되는 것을 특징으로 하는 프럭토스(fructose)에서 타가토스(tagatose)를 생산하는 방법.13. The method according to claim 12, wherein the enzyme reaction is carried out intracellularly. 삭제delete
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