KR101618309B1 - Composition for anti-allergy and skin hydration comprising pterocarpus santalinus extracts - Google Patents

Abstract

Disclosed in the present invention is an anti-allergic skin moisturizing composition comprising a Pterocarpus santalinus extract as an active component. In an aspect, provided in the present invention are a pharmaceutical composition, cosmetic composition and food composition for antiallergy, comprising a Pterocarpus santalinus extract which has no side effects and has high stability, as an active component. In another aspect, provided in the present invention are a topical composition for skin, a cosmetic composition, and a food composition, comprising the Pterocarpus santalinus extract which has no side effects and has high stability, as an active component, for moisturizing the skin, enhancing a skin barrier, or increasing skin elasticity.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for anti-allergy and skin moisturizing, comprising an extract of Autumnal oriental extract as an active ingredient,

Disclosed herein is a composition for skin moisturizing, skin barrier strengthening or skin elasticity enhancement comprising an antiallergic composition comprising a self-directional extract as an active ingredient and a self-directional extract as an active ingredient.

Modern society is becoming increasingly complex, and due to the development of industry and civilization, allergic diseases are increasing every year due to increased pollution of natural environment, change of diet, and stress. In recent years, the National Institute of Allergy and Respiratory Diseases of Japan has used the questionnaire "Childhood and Adolescent Allergic Disease (ISAAC)" to find that atopic dermatitis was 16.3% of elementary school students and 7.3% of middle school students in 1995, And 24.8% and 12.8%, respectively.

Mast cells and blood neutrophils are known to be major body cells that cause various allergic diseases such as atopic dermatitis, allergic rhinitis, asthma, food allergies and anaphylactic shock. These cells have a receptor for IgE (FcεRI), an antibody that induces allergy on the cell surface. These receptors are stimulated by allergen-inducing substances (histamine, Prostaglandins and other allergen-inducing and exacerbating substances are secreted outside the cells (Kim K. et al., Eur. J. Pharmacol., 581: 191-203, 2008).

In studies of atopic dermatitis, also known as allergic disease, the incidence of atopic dermatitis by chemokines has also been reported. These chemokines, like cytokines, are expressed in cells, but their actions are known to be slightly different. That is, the chemokines mainly collect specific cells to specific sites. It is known that when a certain cell expresses a specific chemokine, the kind of cells responding to the chemokine moves toward the cell in which the chemokine is expressed, thereby acting as a sort of aggregation signal. In particular, it is a type of cytokine that regulates the migration and activation of various types of white blood cells, and regulates the infiltration of inflammatory cells into tissues. Specific known chemokines include CCL22 / MDC and CCL17 / TARC (recovery and triggering of T-cells; interleukin 4, interleukin 5, interleukin 13 and T-cell production of TNF- alpha), TSLP (increased production of TARC and MDC ), CCL27 / CTACK (lymphocyte recovery and migration), and RANTES (eosinophil and T-cell migration / activation, increased eosinophil adhesion to the surface of endothelial cells).

Currently, there are various ways to treat allergies, but most of them are merely symptomatic relief rather than fundamentally eliminating the cause. Typically, for the treatment of allergies, medicines containing antagonists against histamine or leukotriene such as histamine or leukotriene secreted from allergens by mast cells are used. However, since such a drug shows tolerance within a short period of time when administered to a patient, there is a problem in that the patient's symptoms can not be improved as in the first administration when administered over a period of time or repeatedly.

The surface of the human skin consists of keratinocytes in which the mature keratinocytes are differentiated from the basal layer and degenerated. These keratinocytes are bound by ceramide double stranded lamellar structure, which is present in the stratum corneum, to maintain a smooth and elastic skin. Ceramide is a type of sphingoid lipid with a fatty acid linked to Sphingosine or Phytosphingosine. Ceramide contains about 40% or more of intercellular lipids constituting the stratum corneum of the skin. It is an essential component for structure formation and function. These ceramides have been reported to have various physiological functions in addition to their properties as a main component of the stratum corneum of the skin.

The keratinocytes are characteristic cells in which the basal cells continuously proliferating in the lowest epidermis undergo a gradual morphological and functional change and rise to the surface of the skin. After a certain period of time, the old keratinocytes are eliminated from the skin And the new keratinocyte replaces its function. This repetitive sequence of changes is called 'epidermal cell differentiation' or 'keratinization'. During keratinization, keratinocytes form a stratum corneum while producing natural moisturizing factor (NMF) and intercellular lipids (ceramide, cholesterol, fatty acid) As shown in Fig. The natural moisturizing factor is responsible for the ability to contain water in the stratum corneum. In the skin cells, a protein called filaggrin is made, and in the stratum corneum, the filagreen is converted to a natural moisturizing factor. The increase in the synthesis of pilgar greens means an increase in natural moisturizing factors, which means improvement of moisturizing function. In addition, the water content in the stratum corneum helps maintain the activity of enzymes having various physiological activities and maintain the skin barrier in a healthy state. Reduced water content in the skin causes damage to the skin barrier, which in turn results in reduced elasticity and wrinkle formation. In other words, the loss of skin moisturizing function causes skin elasticity reduction.

Korean Patent Publication No. 2003-0068967 Korean Patent Publication No. 2007-0013481

In one aspect, the object of the present invention is to provide a composition for anti-allergy comprising an extract of a natural herbal extract derived from a natural product, which has no side effects and is highly stable, as an active ingredient.

In another aspect, the present invention aims to provide a skin moisturizing, skin barrier-strengthening or skin elasticity-enhancing composition comprising a self-directional extract derived from a natural product having no side effects and having high safety as an active ingredient.

In one aspect, the technology disclosed herein provides a pharmaceutical composition for the prevention or treatment of an allergic disease comprising an extract of a self-directional extract as an active ingredient.

In another aspect, the technique disclosed in the present specification provides a cosmetic composition for preventing or improving an allergic disease comprising a self-extracting extract as an active ingredient.

In another aspect, the techniques disclosed herein provide a food composition for preventing or ameliorating an allergic disease, wherein the composition contains a self-directional extract as an active ingredient.

In another aspect, the technique disclosed in this specification provides a skin external application composition for skin moisturization, skin barrier enhancement or skin elasticity enhancement comprising a self-extracting extract as an active ingredient.

In another aspect, the technology disclosed in this specification provides a cosmetic composition for skin moisturizing, skin barrier strengthening or skin elasticity enhancement comprising a self-directional extract as an active ingredient.

In another aspect, the present invention provides a food composition for skin moisturizing, skin barrier strengthening or skin elasticity enhancement comprising a self-directional extract as an active ingredient.

In one exemplary embodiment, the autotextile extract may be extracted with one or more extraction solvents selected from the group consisting of water and an alcohol having 1 to 6 carbon atoms.

In an exemplary embodiment, the autotextile extract may be an ethanol extract.

In one exemplary embodiment, the autologous oriental extract may be contained in an amount of 0.001 to 80% by weight based on the total weight of the composition.

In an exemplary embodiment, the allergic disease is selected from the group consisting of edema, anaphylaxis, allergic rhinitis, allergic asthma, hay fever, Quincke's edema, Allergic conjunctivitis, allergic keratitis, allergic dermatitis, atopic dermatitis, contact dermatitis, hives, pruritus, allergic conjunctivitis, allergic conjunctivitis, allergic keratitis, , Insect allergies, food allergies, and drug allergies.

In an exemplary embodiment, the composition comprises: a) degranulation of mast cells; b) production of histamine; c) production of TNF-a; d) production of interleukins; And e) production of TARC (Thymus and activation-regulated chemokine / CCL17).

In an exemplary embodiment, the composition comprises: a) expression of a pill green; And b) expression of a ceramide synthase.

In one aspect, the present invention has the effect of providing a composition for anti-allergy comprising an extract of a self-orienting oriental herbal extract having no side effects and high stability as an active ingredient.

In another aspect, the present invention has an effect of providing a composition for skin moisturizing, skin barrier strengthening or skin elasticity enhancement comprising an extract of a self-orienting oriental extract having no side effects and high stability as an active ingredient.

FIG. 1 shows the results of confirming that the extract of the autogranular gland suppresses the secretion of allergen-inducing substances present in granules of mast cells.
FIG. 2 shows the result of confirming that the extract of autogranular gland suppresses histamine production in mast cells.
FIG. 3 shows the results of the results of confirming that the extract of autogranular ganglia inhibits TNF-α production in mast cells.
FIG. 4 shows the result of confirming that the extract of autogranularis inhibits interleukin-4 production in mast cells.
FIG. 5 shows the result of confirming that the extract of autologous taraxacillum suppresses TARC production in human keratinocytes.
FIG. 6 shows the result of confirming that the extract of the autologous filamentous root increases the expression of filamentous keratinocytes in human keratinocytes.
FIG. 7 shows the results of confirming that the extract of the male oriental extract increased ceramide synthase 4 (CERS4) expression in human keratinocytes.

Hereinafter, the present invention will be described in detail.

In one aspect, the technology disclosed herein provides a pharmaceutical composition for the prevention or treatment of an allergic disease comprising an extract of a self-directional extract as an active ingredient.

In another aspect, the technique disclosed in the present specification provides a cosmetic composition for preventing or improving an allergic disease comprising a self-extracting extract as an active ingredient.

In another aspect, the techniques disclosed herein provide a food composition for preventing or ameliorating an allergic disease, wherein the composition contains a self-directional extract as an active ingredient.

In another aspect, the technique disclosed in this specification provides a skin external application composition for skin moisturization, skin barrier enhancement or skin elasticity enhancement comprising a self-extracting extract as an active ingredient.

In another aspect, the technology disclosed in this specification provides a cosmetic composition for skin moisturizing, skin barrier strengthening or skin elasticity enhancement comprising a self-directional extract as an active ingredient.

In another aspect, the present invention provides a food composition for skin moisturizing, skin barrier strengthening or skin elasticity enhancement comprising a self-directional extract as an active ingredient.

As used herein, the term "active ingredient" refers to a component that can exhibit the desired activity alone, such as a carrier that exhibits the desired activity alone or is itself inactive.

In the present specification, "allergic disease" is a concept of the best known as a change in body which is caused by a change in an in vivo reaction which is caused by an antigen-antibody reaction when an organism comes into contact with an allergen. In this specification, there is no limitation on the kind of the adventitious substance, and it may include, but not limited to, pollen, drug, vegetable fiber, bacteria, food, dye or chemical. In this specification, the specific parts of the body which are changed due to the adventitious substance are not limited. In the present specification, the term "allergic disease" is meant to include changes in the body caused by changes in the in vivo response to endogenous factors such as hormonal changes, stress, emotional disturbance, fatigue and insufficient sleep.

In an exemplary embodiment, the allergic disease is selected from the group consisting of edema, anaphylaxis, allergic rhinitis, allergic asthma, hay fever, Quincke's edema, Allergic conjunctivitis, allergic keratitis, allergic dermatitis, atopic dermatitis, contact dermatitis, hives, pruritus, allergic conjunctivitis, allergic conjunctivitis, allergic keratitis, , Insect allergies, food allergies, and drug allergies. However, the present invention is not limited thereto, and includes all the changes in the living body caused by adventitious factors or endogenous factors.

Red sandalwood is an evergreen tree ( Pterocarpus santalinus ) belonging to the rosewood bean family of the dicotyledonous plant. The core of the rosewood tree is purple and is hard as a birch tree, so it is called red sandalwood. Wood is also used as furniture and building materials.

In the present specification, the term "self-direction" refers to the woody part of the rosewood, which is the soybean plant. The core material of the rosewood tree (the dark part inside the wood) has been used as a medicinal herb and is called " (Palevitch & Yaniv, Oryx Press, Arizona, 1986) has been used in Korea as a medicinal plant for the treatment of inflammation, mental disorders, and ulcers in the private sector.

The endosymbiont may be used without limitation throughout the entire range of plants including part or all of its overhead or underground for the production of the extract. In addition, the above-mentioned self-directing can be used without restrictions such as cultivation or market.

In one exemplary embodiment, the endomedial extract is meant to include all forms of the extract, as well as any form of the extract, such as dried, concentrated, fractionated, fermented, etc., by further processing.

In an exemplary embodiment, the endomorphism extract may be a self-directed end alone or an extract obtained by pulverizing it or obtaining it through an extraction process. The extraction process may be carried out according to a conventional method used in the art.

In one exemplary embodiment, the autologous oriental extract is selected from the group consisting of water, anhydrous or functional alcohols having 1 to 6 carbon atoms (e.g., methanol, ethanol, propanol or butanol), propylene glycol, butylene glycol, dipropylene glycol, glycerin, Extracted with an extraction solvent selected from the group consisting of acetone, chloroform, methylene chloride, butyl acetate, diethyl ether, dichloromethane, hexane and mixtures thereof, in particular water, methanol and ethanol Lt; / RTI > The extraction solvent is not limited to the extraction solvents listed above, and the specific use amount of the extraction solvent is 5 to 100 times the weight of each sample to be extracted.

In one illustrative embodiment, the autologous extract may be obtained by a method selected from the group consisting of supercritical extraction, subcritical extraction, high temperature extraction, high pressure extraction, ultrasonic extraction, fermentation using natural microorganisms or natural fermentation or adsorption resin containing XAD and HP-20 May be prepared according to a conventional extraction method in the art. Specifically, it may be warmed and refluxed or extracted at room temperature, but is not limited thereto. The extraction frequency may be 1 to 5 times, but it may be extracted three times in detail, but is not limited thereto. The extraction time can be from 2 to 24 hours, in particular from 2 to 12 hours, or from 3 to 10 hours, or from 3 to 5 hours, but is not limited thereto.

In one exemplary embodiment, the autologous oriental extract may be contained in an amount of 0.001 to 80% by weight based on the total weight of the composition. The inclusion of the autologous oriental extract within the above-mentioned range in the composition has an appropriate composition ratio with other ingredients and has excellent antiallergic effect, skin moisturizing effect, skin barrier strengthening effect and skin elasticity strengthening effect, have. Specifically, the autotalpe extract is present in an amount of 0.005 to 78 wt.%, Or 0.01 to 76 wt.%, Or 0.05 to 74 wt.%, Or 0.1 to 72 wt.%, Or 0.5 to 70 wt.%, Or 1.0 to 68 wt. Or 1.0 to 66 wt%, or 1.0 to 64 wt%, or 1.0 to 62 wt%, or 1.0 to 60 wt%, or 1.0 to 50 wt%, or 1.0 to 40 wt%, or 1.0 to 30 wt% %, Or 1.0 to 20 wt%, or 1.0 to 10 wt%.

The composition according to the present disclosure can inhibit histamine production. The composition may inhibit or inhibit the production of histamine itself or may inhibit or inhibit the activity of the histamine produced by the composition of the present disclosure. In another aspect, the composition may inhibit or inhibit the activity of an up-stream enzyme or protein that produces histamine.

In addition, the composition according to the present specification can inhibit the production of interleukin. The composition may inhibit or inhibit the production of the interleukin itself or may inhibit or inhibit the activity of the interleukin that is generated insignificantly by the compositions herein. In another aspect, the composition may inhibit or inhibit the activity of an up-stream enzyme or protein that produces interleukin. In one exemplary embodiment, the interleukin includes, but is not limited to, interleukin 4, interleukin 5, interleukin 13.

On the other hand, TARC (Thymus and activation-regulated chemokine / CCL17) is a type of chemokine involved in leukocyte migration (migration). Atopic dermatitis has migrating (migrating) activity to Th2 cells expressing TARC receptor CCR4. TARC production is enhanced in skin epidermal keratinocytes and in peripheral blood mononuclear cells, and excessively produced TARC It is believed that Th2 cells expressing CCR4 migrate to the skin and exacerbate the condition. The serum TARC levels in patients with atopic dermatitis are significantly higher than those of other skin diseases and increase with the severity of atopic dermatitis. Thus, TARC can be a key indicator for objectively evaluating the condition of atopic dermatitis.

The composition according to the present disclosure can inhibit the production of TARC. The composition may inhibit or inhibit the production of the TARC itself or may inhibit or inhibit the activity of the TARC that is generated negligible by the compositions herein. In another aspect, the composition may inhibit or inhibit the activity of an up-stream enzyme or protein that produces TARC.

A composition for improving, alleviating or treating an existing allergic disease can not fundamentally solve the cause of allergy by using an antagonistic antibody against a receptor of an already generated and secreted allergen causing substance. However, Which can be a fundamental solution for the prevention of allergic diseases and the like. Accordingly, the composition for anti-allergy according to the present invention can be usefully used as a composition having an excellent effect for preventing, improving or treating allergic diseases such as asthma, atopic dermatitis, rhinitis and the like.

In addition, the composition according to the present disclosure can increase the production of filla greens. The composition may inhibit or inhibit the activity of an up-stream enzyme or protein that produces pillar green.

In addition, the composition according to the present disclosure may increase the production of ceramide synthase (CERS). The composition may inhibit or inhibit the activity of an up-stream enzyme or protein that produces CERS. The CERS includes, but is not limited to, CERS1, CERS2, CERS3, CERS4, CERS5, CERS6.

The keratinocytes form a stratum corneum while producing intercellular lipids such as natural moisturizing factors and ceramides, thereby enabling the stratum corneum to function as an enhanced skin barrier with firmness and elasticity of elasticity and moisturization. The composition according to the present invention increases the amount of ceramide synthetic enzyme which is a natural moisturizing factor and skin barrier component to enhance skin moisturizing and skin elasticity, thereby providing skin moisturizing, skin barrier enhancement and skin elasticity enhancement.

In this specification, the pharmaceutical composition may be one comprising an external preparation for skin. Formulations of the pharmaceutical compositions may be, but are not limited to, solutions, suspensions, emulsions, gels, suspensions, suppositories, creams, ointments, patches, pads or spraying agents. The formulations can be readily prepared according to conventional methods in the art and can be prepared by conventional means such as excipients, wetting agents, emulsifying accelerators, suspending agents, salts or buffers for controlling osmotic pressure, coloring agents, spices, stabilizers, preservatives, Adjuvants may be used as appropriate.

In addition, the pharmaceutical composition may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously, etc. depending on the intended method, and the active ingredient of the pharmaceutical composition may be appropriately selected depending on the age, Sex, weight, pathology and severity thereof, route of administration, or judgment of the prescriber. Determination of the amount of application based on these factors is well within the level of ordinary skill in the art and its daily dose is, for example, from 0.1 mg / g / day to 100 mg / g / day, more specifically from 5 mg / g / day to 50 mg / g / day. < / RTI >

In the present specification, the formulation of the cosmetic composition is not particularly limited, and can be appropriately selected according to the purpose. For example, it may be formulated into a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an oil phase in water, a suspension, a solid, a gel, a powder, a paste, a foam or an aerosol composition But is not limited to.

In addition, the cosmetic composition may further contain, in addition to the above-mentioned substances, other ingredients which can give a synergistic effect to the main effect, without impairing the main effect. The cosmetic composition may further include a moisturizing agent, an emollient agent, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic and inorganic pigment, a fragrance, a cold agent or a limiting agent. The blending amount of the above components can be easily selected and used by those skilled in the art within a range not to impair the objects and effects of the present invention. The blending amount thereof is 0.01 to 5% by weight, or 0.01 to 3% .

Food compositions herein provide various forms of food additives or functional foods. Specifically, the composition can be processed into an extruded tea, a liquid tea, a beverage, a fermented milk, a cheese, a yogurt, a juice, a probiotic agent or a health supplement, etc., and can be used in various other food additives.

In addition, the food composition may further contain other ingredients and the like that can give a synergistic effect to the main effect within a range in which the active ingredient does not impair the intended main effect. For example, additives such as perfume, coloring agent, bactericide, antioxidant, preservative, moisturizing agent, thickening agent, inorganic salt, emulsifier and synthetic polymer substance may be further added for improvement of physical properties. In addition, it may further contain auxiliary components such as water-soluble vitamins, oiliness vitamins, high molecular weight peptides, polymeric polysaccharides and seaweed extract. The ingredients may be selected and mixed by the person skilled in the art without difficulty depending on the purpose of formulation or use, and the amount thereof may be selected within a range that does not impair the objects and effects of the present specification. For example, the amount of addition of the components may range from 0.01 to 5% by weight, or from 0.01 to 3% by weight, based on the total weight of the composition.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

Example 1: Preparation of autologous oriental extract

Pterocarpus santalinus was purchased from Kyungdong market in Seoul and washed and dried. The self-direction was pulverized and placed in an extraction container and an appropriate amount of ethanol was added. These were allowed to stand at room temperature for 7 days, and then filtered with a filter paper to obtain an autogranular extract. Specifically, 1 L of ethanol was added to 100 g of vertically orientated oriental extract, followed by extraction and concentration to obtain 14.2 g of autologous oriental extract. The thus obtained autographed extract was dissolved in DMSO (dimethyl sulfoxide) and used in the following experimental examples.

Experimental Example 1. Inhibition of secretion of allergens present in granules of mast cells

(Jeong, H.J. et al., Cytokine, 18: 252-9, 2002) whether or not the extract of Zonta orientalis suppresses the secretion of allergens present in the granules of mast cells.

Rat basophilic leukocyte (RBL-2H3, American Type Culture Collection, USA) mast cells were cultured in a minimal medium containing antibiotics and 10% bovine serum. After culturing, the cells were harvested by trypsin, and the cells were added to a 24-well microtiter plate at 2 × 10 ⁵ cells / well and cultured to 80% growth. The thus-cultured cells were substituted with PIPES buffer (25 mM PIPES, pH 7.2, 159 mM NaCl, 5 mM KCl, 0.4 mM MgCl ₂ , 1 mM CaCl ₂ , 5.6 mM glucose, and 0.1% BSA) The extracts obtained from Example 1 were added at concentrations of 5, 10 and 20 μg / ml, respectively, and cultured for 1 hour. One hour later, DNP-BSA was added to a final concentration of 200 μg / ml and stimulation was induced for 30 minutes. The degree of secretion of allergen-inducing substances was determined by measuring the activity of beta-hexosaminidase, a marker of degranulation secreted in the medium. The activity of beta-hexosaminidase was determined by p- The amount of p-nitrophenol liberated from p-nitrophenyl-acetyl-β-D-glucosaminide was determined by the method of Funaba M. et al. Cell Biol. Int., 27: 879-85, 2003).

As a result, as shown in FIG. 1, the extract of the autogranular extract inhibited the secretion amount of β-hexosaminidase, which is a marker of mast cell degranulation, and the inhibitory effect was also increased as the concentration of the self- Respectively. Thus, it was confirmed that the extract of the autogranular exudate had an excellent effect of inhibiting the secretion of allergens present in granules of mast cells.

Experimental Example 2. Inhibition of histamine production

Whether or not the extracts of rhizome extract inhibited histamine production was confirmed as follows.

RBL-2H3 mast cells were cultured in a 24-well microtiter plate containing 2.5% fetal bovine serum (DMEM) (Dulbecco's Modified Eagle's Media) at 2 × 10 ⁵ cells / well , And cultured until it grew to 80%. Then, each of the 5, 10 and 20 μg / ml of the autotextile extracts obtained from Example 1 was added to serum-free DMEM medium, and the mast cells were cultured for 24 hours. The mast cells were treated with DNP-BSA for 30 minutes. Then, the cell culture was collected, centrifuged, and the supernatant was harvested. The amount of histamine in the supernatant was determined using an EIA kit (Bertin Pharma, France).

As a result, as shown in FIG. 2, it was confirmed that the extract of autographed oriental extract significantly inhibited the secretion amount of histamine and had an excellent inhibitory effect on histamine production.

Experimental Example 3. Inhibition of TNF-α production

It was confirmed as follows whether or not the extract of Autogranatum suppresses the production of TNF-α.

RBL-2H3 mast cells were cultured in a 24-well microtiter plate containing 2.5% fetal bovine serum (DMEM) (Dulbecco's Modified Eagle's Media) at 2 × 10 ⁵ cells / well , And cultured to 80% growth. Then, each of the 5, 10 and 20 μg / ml of the autotextile extracts obtained from Example 1 was added to serum-free DMEM medium, and the mast cells were cultured for 24 hours. The mast cells were treated with DNP-BSA for 30 minutes. Then, the cell culture was collected, centrifuged, and the supernatant was harvested. The amount of TNF-a in the supernatant was determined using an ELISA kit (abcam, UK).

As a result, as shown in FIG. 3, it was confirmed that the extract of the autogranular extract suppressed the secretion amount of TNF-α and had an excellent TNF-α production inhibitory effect.

Experimental Example 4. Inhibition of interleukin-4 production

It was confirmed as follows whether or not the extract of the self directed extract inhibits the production of interleukin-4.

RBL-2H3 mast cells were cultured in a 24-well microtiter plate containing 2.5% fetal bovine serum (DMEM) (Dulbecco's Modified Eagle's Media) at 2 × 10 ⁵ cells / well , And cultured to 80% growth. Then, each of the 5, 10 and 20 μg / ml of the autotextile extracts obtained from Example 1 was added to serum-free DMEM medium, and the mast cells were cultured for 24 hours. The mast cells were treated with DNP-BSA for 30 minutes. Then, the cell culture was collected, centrifuged, and the supernatant was harvested. The amount of interleukin-4 in the supernatant was determined using an ELISA kit (abcam, UK).

As a result, as shown in FIG. 4, it was confirmed that the extract of autographed oriental extract suppresses the secretion amount of interleukin-4 and has an excellent inhibitory effect on interleukin-4 production.

Experimental Example 5: Suppression of TARC production

It was confirmed whether the extract of Tetrafacos extract inhibited TARC production as follows .

HaCaT human keratinocytes were seeded at 2 × 10 ⁵ cells / well in a 24-well microtiter plate containing 2.5% fetal bovine serum-containing DMEM (Dulbecco's Modified Eagle's Media) , And cultured to 90% growth. Subsequently, the concentrations of the autotuning extracts obtained from Example 1 at 1, 5 and 10 μg / ml were added to serum-free DMEM medium together with TNF-α and IFN-γ 10 ng / Lt; / RTI > for 24 hours. After 24 hours, the cell culture was collected, centrifuged and only the supernatant was harvested. The amount of TARC in the supernatant was determined using an ELISA kit (abcam, UK).

As a result, as shown in Fig. 5, it was confirmed that the extract of the autologous oriental extract had an excellent effect of inhibiting TARC production.

Experimental Example 6. Increase in the amount of pilar green expression

The effect of the extracts of P. orientalis on the expression level of pilar green was confirmed as follows .

First, human keratinocytes were cultured in a 6-well microtiter plate containing 2.5% fetal bovine serum-containing DMEM (Dulbecco's Modified Eagle's Media) at a density of 2 × 10 ⁶ cells / well ), And cultured until it grew to about 90%. Subsequently, each of the autologous extract 5 obtained in Example 1 and 10 μg / ml of each of TNF-α and IFN-γ was added to serum-free DMEM medium together with 10 ng / ml of IFN-γ, Lt; / RTI > After 24 hours, total RNA was harvested from the cells using TRIzol reagent (Invitrogen, Carlsbad, Calif., USA) and reverse transcribed, and RT-PCR analysis was performed as follows. First, the RNA was reverse transcribed using reverse transcriptase for cDNA synthesis. RT-PCR was performed with the following specific primers:

β-actin

Forward primer: 5'-AGCCATGTACGTAGCCATCC-3 '(SEQ ID NO: 1)

Reverse primer: 5'-CTCTCAGCTGTGGTGGTGAA-3 '(SEQ ID NO: 2)

Filaggrin

Forward primer: 5'-AGTGCACTCAGGGGGCTCACA-3 '(SEQ ID NO: 3)

Reverse primer: 5'-CCGGCTTGGCCGTAAGTGTGT-3 '(SEQ ID NO: 4)

Each relative mRNA expression level was normalized to a beta -actin value.

As a result, as shown in FIG. 6, it was found that the extract of the autographed oriental extract remarkably increased the expression amount of pilgar green. Accordingly, the extract of the self-directional extract of the present invention increased the natural moisturizing factor of the stratum corneum to improve the skin moisturization and strengthen the skin barrier, thereby improving the skin health and improving the skin.

EXPERIMENTAL EXAMPLE 7 Increase in Expression of Ceramide Synthase

The effect of the extracts on the expression of Ceramide synthase 4 (CERS4) was confirmed as follows .

First, human keratinocytes were cultured in a 6-well microtiter plate containing 2.5% fetal bovine serum-containing DMEM (Dulbecco's Modified Eagle's Media) at a density of 2 × 10 ⁶ cells / well ), And cultured until it grew to about 90%. Subsequently, each of the autologous extract 5 obtained in Example 1 and 10 μg / ml of each of TNF-α and IFN-γ was added to serum-free DMEM medium together with 10 ng / ml of IFN-γ, Lt; / RTI > After 24 hours, total RNA was harvested from the cells using TRIzol reagent (Invitrogen, Carlsbad, Calif., USA) and reverse transcribed, and RT-PCR analysis was performed as follows. First, the RNA was reverse transcribed using reverse transcriptase for cDNA synthesis. RT-PCR was performed with the following specific primers:

β-actin

Forward primer: 5'-AGCCATGTACGTAGCCATCC-3 '(SEQ ID NO: 1)

Reverse primer: 5'-CTCTCAGCTGTGGTGGTGAA-3 '(SEQ ID NO: 2)

CERS4

Forward primer: 5'-TGGCCATGCGCCTTGCCTTT-3 '(SEQ ID NO: 5)

Reverse primer: 5'-GGCGTCTCCGGAACCATCGC-3 '(SEQ ID NO: 6)

Each relative mRNA expression level was normalized to a beta -actin value.

As a result, as shown in FIG. 7, the autoganotic extract promoted the expression of ceramide synthase, which is an essential ingredient for showing the structure and function of the stratum corneum, to maintain smooth and elastic skin, thereby enhancing skin moisturization, And it was confirmed that there is an excellent effect in improving the elasticity.

Formulations and formulations of compositions according to one aspect of the invention are described below, but are also applicable to various other formulations and formulations, which are not intended to be limiting but merely illustrative of the invention.

[Formulation Example 1] Soft capsule

The soft capsules were prepared by mixing 150 mg of the extract of Momordicae orientalis, 2 mg of palm oil, 8 mg of palm kernel oil, 4 mg of yellowing oil, and 6 mg of lecithin and 400 mg per capsule according to a conventional method.

[Formulation Example 2] Tablets

The granules were formed by mixing 150 mg of the extract of Momotaro virginicum, 100 mg of glucose, 50 mg of red ginseng, 96 mg of starch and 4 mg of magnesium stearate, and 30% ethanol was added thereto, followed by drying at 60 ° C. Respectively.

[Formulation Example 3] Granules

The granules were formed by mixing 150 mg of extract from the genus orientalis, 100 mg of glucose, 50 mg of red ginseng extract and 600 mg of starch and 100 mg of 30% ethanol, and dried at 60 ° C. to form granules. The final weight of the contents was 1 g.

[Formulation Example 4] Drinking agent

150 mg of the extract of Momordicae orientalis, 10 g of glucose, 50 mg of red ginseng extract, 2 g of citric acid and 187.8 g of purified water were mixed and filled in a bottle. The final volume of the contents was adjusted to 200 ml.

[Formulation Example 5] Preparation of health food

Autumnal extract: 1000 mg

Vitamin mixture

Vitamin A Acetate ............. 70 ㎍

Vitamin E ..................... 1.0 mg

Vitamin B1 ..................... 0.13 mg

Vitamin B2 .................... 0.15 mg

Vitamin B6 ...................... 0.5 mg

Vitamin B12 ..................... 0.2 g

Vitamin C ....................... 10 mg

Biotin ......................... 10 μg

Nicotinic acid amide ... 1.7 mg

Folic acid ............................ 50 ㎍

Calcium pantothenate ................... 0.5 mg

Mineral mixture

Ferrous sulfate ........................ 1.75 mg

Zinc oxide ......................... 0.82 mg

Magnesium carbonate ..................... 25.3 mg

Potassium phosphate monohydrate 15 mg

Secondary calcium phosphate ...................... 55 mg

Potassium citrate ....................... 90 mg

Calcium carbonate ......................... 100 mg

Magnesium chloride ..................... 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

[Formulation Example 6] Preparation of health drink

Autumnal extract: 1000 mg

Citric acid ...................... 1000 mg

Oligosaccharides ...................... 100 g

Plum concentrate ..................... 2 g

Taurine ......................... 1 g

Purified water was added to the entire mixture to make 900 ml

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was stirred and heated at 85 ° C for about 1 hour. The resulting solution was filtered and sterilized in a 2-liter sterile container. The resulting solution was refrigerated, Can be used for the preparation of a composition.

Although the composition ratio is relatively mixed with a component suitable for a favorite drink, it is also possible to arbitrarily modify the blending ratio according to the regional or national preference such as the demand level, demand country, use purpose, and the like. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

[Formulation Example 1] Softening lotion (skin lotion)

Table 1.

[Formulation Example 2] Softening lotion (milk lotion)

Table 2.

[ Formulation Example  3] Nourishing cream

Table 3.

[ Formulation Example  4] Massage cream

Table 4.

[Formulation Example 5] Pack

Table 5.

[Formulation Example 6] ointment

Table 6.

Having described specific portions of the present invention in detail, it will be apparent to those skilled in the art that this specific description is only a preferred embodiment and that the scope of the present invention is not limited thereby. It will be obvious. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (12)

delete delete delete delete A cosmetic composition for enhancing skin barrier comprising Pterocarpus santalinus extract as an active ingredient and promoting ceramide lipid synthesis.
A composition for enhancing skin barrier comprising an extract of Pterocarpus santalinus as an active ingredient and promoting ceramide lipid synthesis.
The method according to claim 5 or 6,
Wherein the autotuning extract is extracted with at least one extraction solvent selected from the group consisting of water and an alcohol having 1 to 6 carbon atoms.
The method according to claim 5 or 6,
Wherein the autotextile extract is an ethanol extract.
The method according to claim 5 or 6,
Wherein said autotuning extract is comprised between 0.001 and 80% by weight based on the total weight of the composition.
The method according to claim 5 or 6,
Wherein the composition enhances the expression of pillar green to improve skin moisturization. delete delete

Images