KR101608942B1 - Optical microscope using a retroreflective sheet and its operation method - Google Patents

Abstract

The present invention relates to an optical microscope using a retroreflective sheet, and a method to operate the same. The optical microscope of the present invention comprises: a light generation unit to generate light; a stage provided on a light path generated from the light generation unit, having a transparent preparat of a measurement target fixated thereto, and made of a transparent material to transmit a portion of the light generated from the light generation unit; an optical system provided between the stage and the light generation unit, and providing the light path to make the light, which is generated from the light generation unit, incident on stage; a retroreflective sheet for retroreflecting the light transmitted through the stage and the preparat; and an image sensor to convert the light, which is guided by the optical system to be incident, into an electrical signal to obtain the electrical signal. A portion of the light generated from the light generation unit is directly reflected by the measurement object, and another portion of the light is retroreflected by the retroreflective sheet after transmitting through the measurement target. The optical microscope of the present invention is able to improve a clarity of an image information displayed through a display module by primarily directly reflecting the light generated from the light generation unit by the measurement object; secondarily retroreflecting the light by the retroreflective sheet; and outputting a result image information by placing the reflected light together.

Description

TECHNICAL FIELD [0001] The present invention relates to an optical microscope using a retroreflective sheet, and a method of operating the optical microscope.

The present invention relates to an optical microscope using a retroreflective sheet and a method of operating the same.

More specifically, a part of the light generated in the light generating part is directly reflected by the object to be measured, which is imaged on the transparent prepalat, and the other part of the light is reflected by the retroreflective sheet after passing through the object to be measured, Reflected or retroreflected light relates to an optical microscope using a retroreflective sheet that can be viewed and synthesized by an image sensor or eyepiece and its method of operation.

In general, a microscope is a type of magnifying glass that is used to observe microstructures that can not be determined by the naked eye. These microscopes are used primarily for observing cellular and biological samples in biology, biomedical research, medical diagnostics, and materials science.

Typical types of microscopes include an optical microscope using a glass lens and using visible light, a magnetic lens using an electron microscope with short wavelengths and a cantilever with the surface of the sample being adjacent to each other, There is an atomic microscope that allows the atomic structure to be grasped by using the attraction force and the repulsive force generated between the atom at the end and the atom at the surface of the sample.

In particular, the optical microscope includes a light or bright-field microscope for forming an enlarged image using an objective lens, a phase contrast microscope for easily observing living cells using a difference in refractive index, An interference microscope that can easily observe the structure of a transparent specimen due to an interference phenomenon to a light wavelength by superimposing an object light transmitted through a specimen and an interference light separated from light by using a phenomenon that an object delays light, Using a dark-field microscope and two polarizing prisms (or Nicol prisms) that utilize the phenomenon that light is indirectly visible and easily observed in relatively dark places, A polarizing microscope that uses the principle that light is not transmitted when it is placed and an ultraviolet If light of samples that emit fluorescence principle is divided in such a fluorescence microscope (Fluorescece microscope) using the same.

As one of techniques relating to the above-described optical microscope, a microscope module is disclosed in Registration No. 10-1527925 (registered on June, 2015. 06.04).

1 is a cross-sectional view showing an embodiment of a conventional microscope apparatus. Referring to FIG. 1, a conventional microscope apparatus can be used to simultaneously implement an inverted microscope and an orthopedic microscope using a single microscope, and can be used as an inverted microscope and an orthopedic microscope according to the convenience of the user have.

The conventional microscope apparatus includes a stage 1 on which a sample is placed, a light unit 2 for irradiating light onto a sample on the stage 1, light passing through the sample is refracted, A lens barrel 4 for transmitting light that has passed through the objective lens unit 3, an eyepiece lens 4 disposed at one end of the lens barrel 4 for allowing a user to view an image of the sample, And a frame 6 on which the stage 1, the light portion 2 and the objective lens portion 3 are supported and the position of the light portion 2 and the objective lens portion 3 are formed on the stage 5, (1) so that observations on an on-state and an orthographic view can be selectively made.

However, in the conventional technique, the positions of the light portion 2 and the objective lens portion 3 are rotated about the stage 1, so that the observation of the sample placed on the stage 1 can be made to be a sphere However, since there is no configuration for fixing the frame 6 after rotation, the positions of the light unit 2 and the objective lens unit 3 are not fixed, so that the image of the object may be shaken.

In addition, since the structure of the reflector for reflecting the light irradiated from the light portion 2 and observing the sample placed on the stage 1 clearly is not described, it is difficult to improve the sharpness for observing the sample there is a problem.

Registered Patent Publication No. 10-1527925 (June 05, 2015) Published Patent Publication No. 10-2015-0092296 (Aug. 12, 2015) Published Japanese Patent Application No. 10-2013-0030686 (Feb. Patent Registration No. 10-1157791 (2012. 06. 13.)

SUMMARY OF THE INVENTION The present invention has been conceived to solve the above problems, and it is an object of the present invention to provide a light source apparatus and a light source apparatus, in which a part of light generated in a light generating unit is directly reflected by a measurement object, And a retroreflective sheet which is retroreflected by a retroreflective sheet after passing through a measurement object and a method of operating the optical microscope.

Another object of the present invention is to provide an image sensor which is directly reflected by an object to be measured or that converts retroreflected light to an electric signal by a retroreflective sheet, An optical microscope using a reflective sheet, and a method of operation thereof.

Another object of the present invention is to provide an optical microscope and a method of operating the same using a retroreflective sheet that can be directly reflected by an object to be measured or retroreflected by a retroreflective sheet, .

Another object of the present invention is to provide a retroreflective sheet having at least one of a planar shape, a concave shape and a hemispherical shape and having an optical filter for allowing only light of a specific wavelength band to pass through the light generated in the light generating unit And an ascending and descending means for ascending and descending the retroreflective sheet. The present invention also provides an operation method of the optical microscope.

The objects of the embodiments of the present invention are not limited to the above-mentioned objects, and other objects not mentioned can be clearly understood by those skilled in the art from the following description .

According to an aspect of the present invention, there is provided an optical microscope using a retroreflective sheet, comprising: a light generating unit generating light; A light fixture provided on the path of light generated by the light generator and fixed to a transparent preparate on which a measurement object is sampled, a transparent material made of a transparent material, An optical system provided between the lifting bar and the light generating part and providing a path of the light so that the light generated in the light generating part is incident on the lifting bar; A retroreflective sheet for retroreflecting the light transmitted through the flooring and the prepalam; And an image sensor which is obtained by converting the light introduced by the optical system into an electric signal and obtaining the light, wherein a part of the light generated in the light generating unit is directly reflected by the measurement object, And the other part of the light is retroreflected by the retroreflective sheet after passing through the measurement object.

Here, the image sensor may be a charge coupled device (CCD) sensor or a complementary metal oxide semiconductor (CMOS) sensor.

An image processing module for converting an electric signal obtained through the image sensor into resultant image information and outputting the resultant image information; And a display module for displaying the result image information output from the image processing module, wherein the control module controls the display module to display the result image information output from the image processing module through the display module.

Further, the retroreflective sheet is characterized by having at least one of a planar shape, a concave shape, and a hemispherical shape.

The optical filter may further include an optical filter for passing only light of a specific wavelength band among the light generated by the light generating unit.

It is further characterized in that the retroreflective sheet is further provided with an up-and-down means for raising and lowering the retroreflective sheet in the up-and-down direction to adjust the distance between the retroreflective sheet and the stay.

According to another aspect of the present invention, there is provided a method of operating an optical microscope using a retroreflective sheet, comprising: generating light in a light generating unit; The light generated in the light generating unit is incident on an optical system and guided to a path provided by the optical system; A part of the light guided by the optical system is directly reflected by the object to be measured, and another part of the light guided by the optical system is transmitted through the object, the preparate and the object to be measured; The reflections being reflected by the retroreflective sheet, the refractory sheet and the light passing through the object to be measured; Transmitting the retroreflected light by the retroreflective sheet to the ground, the prepalatal and the object to be measured; And a step of converting the light directly reflected by the measurement object and the light retroreflected by the retroreflective sheet into an electrical signal by an image sensor, and obtaining the optical signal, wherein when the sample to be measured is transparent or translucent , A part of the light guided by the optical system is directly reflected by the object to be measured, the other part of the light is transmitted through the object to be measured and then retroreflected by the retroreflective sheet, And when it is opaque, the light induced by the optical system is directly reflected by the object to be measured.

Generating resultant image information using the electrical signal obtained from the image sensor; And displaying the resultant image information through a display module.

According to another aspect of the present invention, there is provided an optical microscope using a retroreflective sheet, comprising: a light generating unit generating light; A light fixture provided on the path of light generated by the light generator and fixed to a transparent preparate on which a measurement object is sampled, a transparent material made of a transparent material, An optical system provided between the lifting bar and the light generating part and providing a path of the light so that the light generated in the light generating part is incident on the lifting bar; A retroreflective sheet for retroreflecting the light transmitted through the flooring and the prepalam; And an eyepiece (80) in which image information of light incident and guided by the optical system is observed, and a part of the light generated in the light generating unit is directly reflected by the measurement object, and another part Is reflected by the retroreflective sheet after passing through the object to be measured.

The eyepiece lens may further include: a first lens; And a second lens spaced apart from the first lens, the second lens being disposed at a position aligned with the center axis of the first lens.

Further, the first lens and the second lens are each formed in at least one of a planar shape, a concave shape, and a convex shape.

According to the optical microscope using the retroreflective sheet according to the embodiment of the present invention, light generated in the light generating unit is directly reflected first by the object to be measured, secondarily retroreflected by the retroreflective sheet, By outputting the resultant image information, the sharpness of the image information displayed through the display module can be improved.

According to the present invention, since the light emitted from the light generating unit is directly reflected by the object to be measured in a first order and retroreflected by the retroreflective sheet in a second order, the sharpness of the image information observed through the eyepiece can be improved There are advantages.

According to the present invention, the retroreflective sheet has at least one of a planar shape, a concave shape, and a hemispherical shape, and includes an optical filter that allows only light of a specific wavelength band to pass through the light generated in the light generating unit, Up and down means for raising and lowering the light is provided to reduce the influence of the reflection efficiency of light and the interference of the light incident from the light generating portion and the light directly reflected by the measurement object and the light retroreflected by the retroreflective sheet .

Further, according to the present invention, the retroreflective sheet has at least one of a planar shape, a concave shape and a hemispherical shape and is vertically moved up and down so that the reflection efficiency of the light retroreflected by the retroreflective sheet can be further improved There is an effect that can be.

1 is a sectional view showing an embodiment of a conventional microscope apparatus;
2 is a perspective view showing an optical microscope using a retroreflective sheet according to an embodiment of the present invention;
3 is a schematic view showing an optical microscope using a retroreflective sheet according to the embodiment of FIG. 2;
4 is a view showing the state of the optical microscope using the retroreflective sheet according to the embodiment of Fig.
5A is a photograph of an experiment using a general optical microscope to which a retroreflective sheet is not applied.
5B is a photograph of an experiment using an optical microscope to which a retroreflective sheet according to an embodiment of the present invention is applied.
FIG. 6 is a flowchart showing an operation method of an optical microscope using a retroreflective sheet according to the embodiment of FIG. 2. FIG.
7 is a perspective view showing an optical microscope using a retroreflective sheet according to another embodiment of the present invention.

While the invention is susceptible to various modifications and alternative forms, specific embodiments thereof are shown by way of example in the drawings and will herein be described in detail. It is to be understood, however, that the invention is not to be limited to the specific embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention.

It is to be understood that when an element is referred to as being "connected" or "connected" to another element, it may be directly connected or connected to the other element, .

On the other hand, when an element is referred to as being "directly connected" or "directly connected" to another element, it should be understood that there are no other elements in between.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In the present application, the term "comprises" or "having ", etc. is intended to specify the presence of stated features, integers, steps, operations, elements, parts, or combinations thereof, And does not preclude the presence or addition of one or more other features, integers, integers, steps, operations, elements, components, or combinations thereof.

Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the meaning in the context of the relevant art and are to be construed as ideal or overly formal in meaning unless explicitly defined in the present application Do not.

Hereinafter, the present invention will be described in more detail with reference to the accompanying drawings. Prior to this, terms and words used in the present specification and claims should not be construed to be limited to ordinary or dictionary meanings, and the inventor should properly interpret the concept of the term to describe its own invention in the best way. The present invention should be construed in accordance with the meaning and concept consistent with the technical idea of the present invention. Further, it is to be understood that, unless otherwise defined, technical terms and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Descriptions of known functions and configurations that may be unnecessarily blurred are omitted. The following drawings are provided by way of example so that those skilled in the art can fully understand the spirit of the present invention. Therefore, the present invention is not limited to the following drawings, but may be embodied in other forms. In addition, like reference numerals designate like elements throughout the specification. It is to be noted that the same elements among the drawings are denoted by the same reference numerals whenever possible.

The present invention is characterized in that a part of the light generated in the light generating part is directly reflected by a measuring object which is sampled in a transparent prepalat and the other part of the light is retroreflected by a retroreflective sheet after passing through the object to be measured, The retroreflected light relates to an optical microscope using a retroreflective sheet which can be observed by being integrated by an image sensor or an eyepiece and a method of operation thereof.

Hereinafter, an optical microscope using a retroreflective sheet according to the present invention and an operation method thereof will be described in detail with reference to the accompanying drawings.

FIG. 2 is a perspective view showing an optical microscope using a retroreflective sheet according to an embodiment of the present invention, and FIG. 3 is a schematic view showing an optical microscope using a retroreflective sheet according to the embodiment of FIG.

2 and 3, an optical microscope using a retroreflective sheet according to an embodiment of the present invention includes a frame 10, a light generating unit 20, a platform 30, an optical system 40, A reflective sheet 50 and an image sensor 60. In the optical microscope according to the embodiment of the present invention, a part of the light generated in the light generating part 20 is directly reflected by the measurement object, and the other part of the light is transmitted through the measurement object, The directly reflected light and the retroreflected light are acquired by the image sensor 60 and synthesized by an image processing module 71 to be described later and transmitted through the display module 72 to the display 50. [ .

The light generating unit 20 generates light and outputs the light to the reflecting mirror 41. The light generated by the light generating unit 20 is reflected by the reflector 41 and illuminates the preparat 31 of the weighing platform 30.

The light generating unit 20 may further include an optical filter 21 for passing only light of a specific wavelength among the light generated by the light generating unit 20.

The lifting bar 30 is provided in a path of light generated by the light generating unit 20 and reflected by the reflecting mirror 41 and is fixed to a transparent prepartat 31 on which a measurement object is sampled. More specifically, the lifting bar 30 is made of a transparent material and is configured to transmit a part of light generated from the light generating part 20.

The lifting frame 30 can be vertically moved up and down by a coarse screw 32 provided on one side of the frame 10 and is vertically adjusted by the coarse screw 32, The focus can be adjusted by the light reflected by the light source.

The optical system 40 includes a reflector 41 disposed between the light generating unit 20 and the ground 30 and reflecting the light generated from the light generating unit 20 to the ground 30, And an objective lens 42 for collecting light reflected by the reflecting mirror 41 and the retroreflective sheet 50. [

The objective lens 42 may be configured such that a plurality of lenses 42a having different magnifications are arranged in the circumferential direction and can be rotated in place to adjust the magnification.

The retroreflective sheet 50 reflects the light transmitted through the stay 30 and the prepartat 31. The retroreflective sheet 50 may have a shape of at least one of a planar shape, a concave shape and a hemispherical shape, and the up / down means 51 for adjusting the distance between the retroreflective sheet 50 and the image sensor 60 . The elevating and lowering means 51 corresponds to the interference caused by the optical path difference when the optical filter 21 is installed. At this time, the raising and lowering means 51 can be applied to a case where the retroreflective sheet 50 is not only a flat, concave or hemispherical shape, but also other shapes such as a convex shape and a dispersion shape.

The raising and lowering means 51 adjusts the interval between the retroreflective sheet 50 and the ground 30 by raising and lowering the retroreflective sheet 50 in the vertical direction, , It can be manually controlled by a raising / lowering screw 52 provided in front of the retroreflective sheet 50 or automatically controlled by a control unit 70 to be described later to raise and lower the retroreflective sheet 50 .

The image sensor 60 converts the incident light into an electrical signal and acquires the electrical signal. The image sensor 60 may be a charge coupled device (CMOS) sensor or a complementary metal oxide semiconductor (CMOS) sensor. The light obtained by converting the image sensor 60 into an electric signal may be a light 30, and the light retroreflected by the retroreflective sheet 50, which is directly reflected by the object to be measured.

Also, the image sensor 60 acquires the direct reflected light by converting it into a primary electrical signal, and acquires the secondary electrical signal by converting the recursively reflected light into a secondary electrical signal.

According to the present invention, the primary electrical signal and the secondary electrical signal are used to explain that each electric signal is sequentially acquired, and it can be understood that it is an electric signal that is actually generated at the same time.

The optical microscope of the present invention includes an image processing module 71 for converting an electric signal obtained through the image sensor 60 into resultant image information and outputting the resultant image information, And a display module 72 for controlling the display module 72. [

The image processing module 71 may combine the primary electrical signal and the secondary electrical signal obtained by the image sensor 60 and convert the result into the resulting image information.

According to the present invention, since the primary electrical signal obtained by being directly reflected and obtained by the measurement object and the secondary electrical signal obtained by retroreflection by the retroreflective sheet 50 are synthesized and converted into the resulting image information, The sharpness of the result image information displayed through the display module 72 can be improved.

4 is a view showing the state of the optical microscope using the retroreflective sheet according to the embodiment of FIG.

As shown in FIG. 4, the optical microscope of the present invention is such that the light generated in the light generating unit 20 is guided to the platform 30 through the objective lens 42 by the reflecting mirror 41. A part of the light guided by the reflecting mirror 41 is directly reflected to the image sensor 60 by the object to be measured which is imaged on the prepartat 31 of the reel 30 and another part of the light is reflected by the reel 30 And the light transmitted through the stretchable material 30 and the prepartat 31 is retroreflected by the retroreflective sheet 50 to the image sensor 60. [

The light directly reflected by the object to be measured and the light retroreflected by the retroreflective sheet 50 are converted into an electric signal in the image sensor 60 and obtained, The electric signal is converted into the resultant image information in the image processing module 71 of the controller 70 and the converted image information converted in the image processing module 71 is supplied to the display module 72 of the controller 70, do.

FIG. 5A is a photograph of an experiment using a general optical microscope to which a retroreflective sheet is not applied, and FIG. 5B is a photograph of an experiment using an optical microscope to which a retroreflective sheet according to an embodiment of the present invention is applied.

As shown in FIGS. 5A and 5B, when an object to be measured is observed with a general optical microscope to which the retroreflective sheet 50 of the present invention is not applied, the shape of the object to be observed observed with an optical microscope is ambiguous, When the object to be measured is observed with an optical microscope to which the retroreflective sheet 50 of the present invention is applied, the shape and color of the object to be observed observed with an optical microscope are clear, and the retroreflective sheet 50 It can be seen that the sharpness is significantly improved when an object to be measured is observed with an optical microscope which is not applied.

6 is a flowchart showing an operation method of the optical microscope using the retroreflective sheet according to the embodiment of FIG.

6, an operation method of an optical microscope using a retroreflective sheet according to an embodiment of the present invention can be performed in eight steps, which includes a light generation step S10, a light induction step S20 A direct reflection and transmission step S30, a retroreflection step S40, a retransmission step S50, an electrical signal conversion step S60, a result image information generation step S70 and a display step S80 .

1. Light generation step (S10)

In the light generation step S10, light is generated in the light generator 20. At this time, the light generated by the light generating unit 20 is filtered by the optical filter 21, and only the light of a specific wavelength band can be passed through.

2. Light induction step (S20)

The light induction step S20 induces light generated in the light generation step S10 to be incident on the optical system 40 to a path provided by the optical system 40. [ At this time, the path provided by the optical system 40 is for allowing the light generated by the light generating unit 20 to emit the flooring 30, and the light can be guided by the reflecting mirror 41.

3. Direct reflection and transmission step (S30)

In the direct reflection and transmission step S30, a part of the light guided by the optical system 40 in the light induction step S20 is directly reflected by the measurement object sampled in the prepalat 31, 40 are transmitted through the platform 30, the prepalat 31 and the object to be measured. This is because when the sample to be measured is transparent or semitransparent to the sample of the preparate 31 and the sample to be measured is opaque to the sample 31, Can be directly reflected.

4. In the reflex reflection step (S40)

In the retroreflecting step S40, the light passing through the platform 30, the prepalat 31 and the object to be measured is retroreflected by the retroreflective sheet 50.

5. Re-permeation step (S50)

The retransmission step S50 is a step in which the retroreflected light in the retroreflecting step S40 retransmits the platform 30, the prepalat 31 and the measurement object.

6. Electrical signal conversion step (S60)

In the electrical signal conversion step S60, the light directly reflected in the direct reflection and transmission step S30 and the light retroreflected in the retroreflection step S40 are converted into an electrical signal by the image sensor 60 . At this time, the image sensor 60 may convert the directly reflected light into a primary electrical signal, and convert the retroreflected light into a secondary electrical signal.

7. Result image information generation step (S70)

The result image information generation step S70 is to generate the result image information using the electrical signal converted by the image sensor 60 in the electrical signal conversion step S60. At this time, the resultant image information may be generated by the image processing module 71, and the image processing module 71 may simultaneously generate the primary and secondary electrical signals converted by the image sensor 60 And the resultant image information can be reconstructed considering the time difference of the primary electrical signal and the secondary electrical signal.

8. Display step (S80)

The displaying step S80 displays the result image information generated by the image processing module 71 in the result image information generating step S70.

7 is a perspective view showing an optical microscope using a retroreflective sheet according to another embodiment of the present invention.

7, an optical microscope using a retroreflective sheet according to another embodiment of the present invention is an optical microscope according to an embodiment of the present invention in which the image sensor is deformed into an eyepiece 80 to be.

The ocular lens 80 of the present invention is guided by the optical system 40 so that the image information of the incident light is observed. The eyepiece 80 may include a first lens 81 and a second lens (not shown) spaced apart from the first lens 81 and aligned with the central axis of the first lens 81, (Not shown) for adjusting an enlargement magnification by adjusting an interval between the first lens 81 and the second lens according to an embodiment of the present invention. At this time, the first lens 81 and the second lens may be at least one of a planar, concave, and convex shape.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It can be seen that branch substitution, modification and modification are possible.

10: frame
20: light generating part 21: optical filter
30: treasure house 31:
32: Coarse thread
40: optical system 41: reflector
42: objective lens 42a: lens
50: Retroreflective sheet 51: Raising and lowering means
52: Raising and lowering screws
60: Image sensor
70: control unit 71: image processing module
72: display module
80: eyepiece lens 81: first lens

Claims (11)

A light generating unit 20 for generating light;
A transparent prepartat 31 on the path of light generated by the light generating part 20 and having a sample to be measured is fixed and is made of a transparent material so that light emitted from the light generating part 20 A lifting base 30 through which a part is transmitted;
An optical system 40 provided between the lifting base 30 and the light generating unit 20 and providing the light path so that the light generated from the light generating unit 20 is incident on the lifting base 30;
A retroreflective sheet (50) for retroreflecting the light transmitted through the platform (30) and the prepartrate (31); And
An image sensor 60 for converting light incident on the optical system 40 into an electric signal to be acquired;
Lt; / RTI >
When the measurement object is transparent or translucent, a part of the light generated in the light generating part 20 is directly reflected by the measurement object, and another part of the light is transmitted through the measurement object, Is retroreflected by the light source 50,
An image processing module 71 for converting an electric signal obtained through the image sensor 60 into result image information and outputting the resultant image information and a display module 72 for displaying result image information outputted from the image processing module 71 And a controller 70 for controlling the display module 72 to display the result image information output from the image processing module 71,
Further comprising an optical filter (21) for allowing only light of a specific wavelength band to pass through the light generated by the light generating unit (20)
The retroreflective sheet 50 is automatically controlled by the control unit 70 to raise and lower the retroreflective sheet 50 in the up and down direction to adjust the distance between the retroreflective sheet 50 and the ground 30, Further comprising a lifting means (51) for preventing lifting of the retroreflective sheet.
The method according to claim 1,
The image sensor (60)
Characterized by comprising a CCD sensor (Charge Coupled Device Sensor) or a CMOS sensor (Complementary Metal Oxide Semiconductor Sensor).
delete The method according to claim 1,
The retroreflective sheet (50)
Wherein at least one of a planar, a concave, and a hemispherical shape is used.
delete delete A light generating unit 20 for generating light;
A transparent prepartat 31 on the path of light generated by the light generating unit 20 and having a sample to be measured is fixed and is made of a transparent material so that light emitted from the light generating unit 20 A lifting base 30 through which a part is transmitted;
An optical system 40 provided between the lifting base 30 and the light generating unit 20 and providing the light path so that the light generated from the light generating unit 20 is incident on the lifting base 30;
A retroreflective sheet (50) for retroreflecting the light transmitted through the platform (30) and the prepartrate (31); And
An ocular lens 80 for observing image information of light guided by the optical system 40;
Lt; / RTI >
A part of the light generated in the light generating part 20 is directly reflected by the measurement object and another part of the light is transmitted through the measurement object and then retroreflected by the retroreflective sheet 50,
Further comprising an optical filter (21) for allowing only light of a specific wavelength band to pass through the light generated by the light generating unit (20)
The retroreflective sheet 50 is automatically controlled by the control unit 70 to raise and lower the retroreflective sheet 50 in the up and down direction to adjust the distance between the retroreflective sheet 50 and the stay 30, Further comprising a lifting / lowering means (51) for preventing lifting of the retroreflective sheet.
The method of claim 7,
The eyepiece lens (80)
A first lens 81; And
A second lens spaced from the first lens 81 and disposed at a position aligned with the center axis of the first lens 81;
Wherein the reflective film is a transparent film.
The method of claim 8,
The first lens (81) and the second lens
Wherein each of the convex portions is formed in at least one of a planar shape, a concave shape, and a convex shape.
delete delete

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