KR101603634B1 - In situ Testing Apparatus for Inhalation Toxicity of Nano Paticles Using Lung Model - Google Patents

Abstract

The present invention relates to a real-time inhalation toxicity testing apparatus using a lung model, and more particularly, to a lung inhalation apparatus and method for constructing a lung model apparatus for performing a similar structure and function to a human lung by attaching lung cells, The inhalation toxicity test for nanoparticles can be carried out indirectly by detecting the change in the electrical signal generated from the lung model device according to the damage state of the lung model in real time, In particular, the present invention provides a real-time inhalation toxicity test apparatus using a lung model capable of grasping the state change of lung cells in real time.

Description

TECHNICAL FIELD [0001] The present invention relates to an inhalation toxicity test apparatus using a lung model,

The present invention relates to a real-time inhalation toxicity test apparatus using a lung model. More particularly, the present invention relates to a lung model device configured to carry out similar structure and function of a human lung by attaching lung cells, and to provide a change in electric signal generated from the lung model device according to the damage state of the lung cell due to inhalation of nanoparticles By real-time detection, it is possible to carry out an inhalation toxicity test on nanoparticles in an indirect manner that grasps changes in the state of lung cells without actually using laboratory animals. In particular, And a real-time inhalation toxicity test apparatus using a lung model.

If the 20th century was the era of micro-era, then the 21st century can be called the Nano era. Nanotechnology can be broadly categorized into nanomaterials, nano devices, and environmental and biotechnology-based technologies, depending on the application field.

These nanotechnologies are artificially manipulated atomic or molecular ultrafine materials to create materials or devices that have new properties and functions that can be used today in information technology (IT) and other biotechnology ) Is a state-of-the-art technology.

However, although nanotechnology offers many benefits and benefits that are perceived as a new technological revolution throughout the industry, it is also a well-known fact that these potential risks It can be attributed to the characteristics of nanotechnology.

That is, the smaller the particle, the larger the ratio of the specific surface area. Small particles having such a large specific surface area ratio increase the toxicity when reacted with the biotissues. For example, some nanoparticles such as titanium dioxide, carbon powder, It has already been revealed through academic experiments that the smaller the size, the more toxic it becomes, such as inflammation. In addition, ultrafine nanoparticles may not penetrate into the airways or mucous membranes but may penetrate deep into the alveoli or migrate to the brain. Moreover, recent studies have shown that nanoparticles accumulate in the body to cause diseases or central nervous disorders .

Therefore, in recent years, along with the development of nanotechnology, stability evaluation of nanotechnology has also been actively carried out. As a representative example, the nanoparticle inhalation toxicity test, which evaluates the toxicity occurring when the nanoparticles are inhaled into the human body, Have been studied. Human toxicity data obtained through these nanoparticle inhalation toxicity tests are being used as various basic data on nanoparticles throughout the industry such as nanofibers, cosmetics, semiconductors, and drug delivery systems.

In recent years, importance of nanotechnology has been emphasized, and various types of tests such as efficacy, safety and environmental impact evaluation of nanoparticles on the human body have been carried out as well as tests for inhalation toxicity of nanoparticles. Are conducted in almost the same manner as the inhalation toxicity test in that they evaluate the influence of the nanoparticles on the human body. Hereinafter, various tests on such nanoparticles are collectively referred to as an inhalation toxicity test.

In addition, since nanoparticles are present in an aerosol state and tests for nanoparticles can be similarly applied to particles having submicron particle diameters in an aerosol state, the nanoparticles are referred to as submicron particles As shown in FIG.

These nanoparticles are very fine in size and can be transferred directly to the lungs deep in the human respiration process and attached to lung tissue. Therefore, the inhalation toxicity test for nanoparticles is generally performed by generating nanoparticles in an aerosol state, supplying the nanoparticles to a predetermined size exposure chamber, exposing the nanoparticles to the exposure chamber, And the like.

In other words, the nanoparticles are exposed to the experimental animals to be inhaled to the deep lungs of the experimental animals, and the nanoparticles are inhaled to the deep lungs, and then the state of health change of the experimental animals is measured.

Such an inhalation toxicity test should be carried out using an experimental animal. In recent years, however, ethical problems with animal experiments have arisen, and the regulations on the test methods using experimental animals are continuously expanding. In addition, there is a problem that it is not easy to provide such a test apparatus because the apparatus for testing toxicity of inhalation using an experimental animal has a large scale, has a high installation and operation cost, and has a complicated structure.

Korean Patent No. 10-1221106

SUMMARY OF THE INVENTION The present invention has been made to solve the problems of the prior art, and an object of the present invention is to provide a lung model device for attaching lung cells to perform structure and function similar to human lungs, The inhalation toxicity test for nanoparticles is performed in an indirect manner in which the change in the electrical signal generated from the lung model device is detected in real time according to the damage state of the cell, And in particular, to provide a real-time inhalation toxicity test apparatus capable of real-time monitoring of changes in lung cell status.

Another object of the present invention is to arrange a plurality of mesh tissue panels in such a manner that a mesh tissue panel having a size smaller in lattice spacing is disposed in order along the inflow direction of nanoparticles, Therefore, it is an object of the present invention to provide a real-time inhalation toxicity test apparatus capable of improving the accuracy of an inhalation toxicity test without using an experimental animal.

The present invention relates to a breathing apparatus comprising a case and a breathing operation unit for introducing the nano particles together with air into the case internal space in such a manner that the inflow and outflow of air into the case internal space are alternately repeated, And a mesh tissue panel disposed in the case inner space and to which each of the lattice lines is attached with human or animal lung cells; And a signal detection unit connected to the mesh tissue panel and detecting an electrical signal generated through the mesh tissue panel in real time. The present invention also provides a real-time inhalation toxicity testing apparatus using the lung model.

At this time, a plurality of the mesh tissue panels are mounted in the case, the plurality of mesh tissue panels are formed to have lattice spaces of different sizes, and the lattice spacing And the mesh tissue panels of the smaller size may be arranged to be sequentially positioned.

The real-time inhalation toxicity testing apparatus may further include: a data processing unit receiving the electrical signal detected by the signal detection unit and determining a damage state of the lung cell; And an output unit outputting a determination result of the data processing unit.

The data processing unit may compare the electrical signal received from the signal detection unit with another database data to determine the damage state of the lung cell.

In addition, the database data may be formed by databaseing the damage state of the lung cells according to an electrical signal generated through the mesh tissue panel.

In addition, the breathing operation unit may include a particle supply module for generating nanoparticles and introducing the nanoparticles into the internal space of the case together with air; And an air exhaust module for exhausting air from the inner space of the case, wherein the particle supply module and the air exhaust module can alternately and repeatedly operate.

The air discharge module may include a buffer bag that is connected to the internal space of the case so that the air introduced into the internal space of the case through the particle supply module may be introduced after passing through the plurality of mesh tissue panels The buffer bag is formed of an elastic material so that the shape of the buffer bag can be restored and the air introduced into the case inner space can be discharged from the inner space of the case by the elastic restoring force of the buffer bag.

The main pipe is connected to the inflow pipe and the discharge pipe so that the air can flow in and out of the case. The inflow pipe is connected to the air in the case, The nano particles may be connected to the particle supply module so that the nanoparticles can flow into the particle supply module. The discharge pipe may have an open end so that air can be discharged from the space inside the case.

In addition, the mesh tissue panel may be formed in such a manner that the human or animal lung cells are uniformly cultured in each grid line.

According to the present invention, a lung model device is constructed so as to perform a structure and function similar to that of a human lung by attaching lung cells, and an electrical signal change generated from a lung model device according to the damage state of lung cells due to inhalation of nanoparticles It is possible to carry out an inhalation toxicity test on nanoparticles in an indirect manner that grasps changes in lung cell status without actually using laboratory animals, There is an effect.

In addition, by disposing the plurality of mesh tissue panels so that the mesh tissue panel having the small size of the grid interval is positioned sequentially along the direction of the flow of the nanoparticles, the lung model device can have a structure similar to the actual lung structure, The accuracy of the inhalation toxicity test can be improved without using an experimental animal.

FIG. 1 and FIG. 2 are conceptual diagrams schematically showing the construction of a real-time inhalation toxicity test apparatus using a lung model according to an embodiment of the present invention,
3 is a functional block diagram conceptually showing a configuration of a real-time inhalation toxicity test apparatus using a lung model according to an embodiment of the present invention,
FIG. 4 is a view schematically showing an arrangement state of a mesh tissue panel according to an embodiment of the present invention;
5 is a cross-sectional view schematically showing the internal structure of a closed model device according to an embodiment of the present invention,
6 and 7 are views schematically showing an operating state of a closed model device according to an embodiment of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the drawings, the same reference numerals are used to designate the same or similar components throughout the drawings. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

1 and 2 are conceptual diagrams schematically showing the configuration of a real-time inhalation toxicity test apparatus using a lung model according to an embodiment of the present invention. Is a functional block diagram conceptually showing the configuration of the toxicity test apparatus.

The inhalation toxicity test apparatus according to an embodiment of the present invention can perform an inhalation toxicity test on nanoparticles without using an actual laboratory animal by using a lung model device operating in a structure similar to a human or animal lung structure As an apparatus, it is an apparatus capable of performing inhalation toxicity evaluation in real time.

First, when we look at the structure and function of human lungs, the lungs are generally half-conical in shape, and there are a pair of left and right lungs that occupy most of the chest cavity facing the mediastinum. Rupture is divided into three lobes in the upper part, and left and right lobes are divided into upper and lower lobes.

These lungs are responsible for the respiratory function, and the left and right lungs are connected to the organs (气管) respectively. The bronchus is divided into left and right bronchus from the height of the fifth thoracic vertebra. The bronchus is divided into the bronchus from the hysterectomy to the lobulated bronchus, and from the lungs back to the bronchus (气管 支 枝) Branching to taper, and finally to the alveoli of the pouch shape. In other words, the parenchyma of the lung is a lot of parcels called alveoli, and the capillary network surrounds this innumerable alveoli, and the gas exchange with the red blood cells is carried out through the alveoli.

During the inspiration process, air enters the lungs from the airway through the organs to the lungs, bronchial tubes, and alveoli. During this intake process, airborne nanoparticles can be inhaled into the lungs of people. In general, large-diameter particles adhere to the trachea or bronchus and are not sucked into deep lungs. However, particles of very small size, such as nanoparticles, And to the alveoli.

The inhalation toxicity test for nanoparticles proceeds through breathing to inhale nanoparticles deep into the lungs of experimental animals and to check for changes in their health status.

A real-time inhalation toxicity testing apparatus using a lung model according to an embodiment of the present invention includes a lung model apparatus 10 having a similar structure and function to that of a human lung, And a signal detection unit 20 for detecting the signal. The data processing unit 30 receives and processes the electric signal detected by the signal detecting unit 20 and further includes an output unit 40 that outputs the data processing result of the data processing unit 30 .

The waste model device 10 includes a case 100 in which a housing space is formed and a case 100 in which air is introduced into and discharged from the interior space of the case 10 in an alternating manner, And a mesh (200), which is formed in a lattice form of a conductive material and is disposed in an inner space of the case (100), and in which lattice cells (C) of a human or an animal are attached, And a tissue panel 300.

According to this structure, the lung model device 10 can perform the breathing function of the lung through the breathing operation unit 200, and the nanoparticles introduced into the case 100 by the breathing operation unit 200 pass Since the mesh-shaped mesh tissue panel 300 to which the lung cells C are adhered is disposed so as to be attachable, it has a structure and function similar to those of actual human or animal lungs.

In this case, one mesh structure panel 300 may be mounted in the inner space of the case 100 as shown in FIG. 1. Alternatively, a plurality of mesh structure panels 300 may be installed in the inner space of the case 100, as shown in FIG. have. The plurality of mesh tissue panels 300 are formed to have lattice spaces of different sizes, and the mesh tissue panel 300 having a smaller grid interval along the inflow direction of nanoparticles in the inner space of the case 100 It is preferable to arrange them so as to be sequentially positioned.

According to this structure, the lung model device 10 has a structure very similar to that of an actual human or animal animal, so that the accuracy of the inhalation toxicity test for nanoparticles can be further improved. Details will be described later.

The signal detection unit 20 is configured to detect an electrical signal generated in real time through the mesh tissue panel 300 in conjunction with the mesh tissue panel 300. In more detail, the mesh tissue panel 300 is formed to have a lattice line 301 of a metallic material, such as copper, and the lattice lines 301 are attached to the tissue of the lung cell C . The signal detection unit 20 is electrically connected to the mesh tissue panel 300 to detect an electrical signal change occurring through the mesh tissue panel 300 in real time.

For example, the signal detection unit 20 can be configured to measure the impedance generated through the mesh organizing panel 300 in real time, and can detect changes in the state of the lung cells C through changes in the impedance value have. That is, the lung cells C adhering to the mesh tissue panel 300 are damaged or changed in activity by the nanoparticles flowing into the inner space of the case 100. In this case, The electric resistance value generated in the line 301 is changed, so that it is possible to measure the change state of the lung cell C by measuring it.

Since the signal detecting unit 20 measures an electric signal generated from the mesh organizing panel 300 in real time, the apparatus for testing an inhalation toxicity according to an embodiment of the present invention is capable of real- (C) can be determined.

The apparatus for testing an inhalation toxicity according to an embodiment of the present invention may further include a data processing unit 30 and an output unit 40. The data processing unit 30 may include a data detection unit 30, And receives the electric signal and processes the data to determine the damage state of the lung cell. The output unit 40 may be configured to output the determination result of the data processing unit 30 in the form of a display device, an audio broadcast, or the like.

At this time, the data processing unit 30 can compare the electric signal applied from the signal detecting unit 20 with another database data to determine the damage state of the lung cell C. The database data may be formed in such a manner that the damage state of the lung cells C is previously databaseized according to the electrical signal value generated through the mesh organizing panel 300.

That is, the electrical signal values generated in accordance with the active state of the lung cell C attached to the mesh tissue panel 300, for example, the degree of lung cell death, 50, and the data processing unit 30 compares the electric signal value measured and applied in real time from the signal detection unit 20 with the electric signal value stored in the data base unit 50 as shown in FIG. 3 , A real-time inhalation toxicity test may be performed in such a manner that the active state of the lung cell C corresponding to the electric signal value is extracted from the database unit 50 and output through the output unit 40.

2 and 3, the signal detection unit 20 can detect an electrical signal for each mesh tissue panel 300 in real time when a plurality of mesh tissue panels 300 are provided As a result, it is possible to discriminate the damage states of the lung cells (C) attached to the respective mesh tissue panels (300), thereby obtaining various types of test results for evaluating the inhalation toxicity of nanoparticles have.

Next, the construction of the closed model device 10 will be described in detail with reference to Figs. 4 to 7. Fig.

FIG. 4 is a schematic view illustrating the arrangement of a mesh tissue panel according to an embodiment of the present invention, FIG. 5 is a cross-sectional view schematically showing an internal structure of a closed model device according to an embodiment of the present invention, 6 and 7 are views schematically showing an operating state of a closed model device according to an embodiment of the present invention.

The lung model device (10) for inhalation toxicity test according to an embodiment of the present invention enables the inhalation of nanoparticles by breathing through a structure similar to that of a human lung, And includes a case 100, a breathing operation unit 200, and a mesh tissue panel 300 as described above.

The case 100 may be formed in a general box shape in which a receiving space is formed therein. Since the airflow generated by the breathing operation unit 200 is generated in the case 100, the internal space may be formed to have a cylindrical shape so as to smoothly flow the air. Alternatively, as shown in FIGS. 1 and 2, Or a polygonal column shape, for example, as shown in FIG.

The breathing operation unit 200 is configured to perform a breathing operation by alternately repeating the inflow and outflow of air into the space of the case 100. Through this breathing operation, air And the nanoparticles are introduced together with the nanoparticles. The breathing operation unit 200 includes a particle supply module 210 for generating nanoparticles and introducing the nanoparticles into the internal space of the case 100 together with air and an air exhaust module for discharging air from the internal space of the case 100 220). The particle supply module 210 performs the inspiratory function of the breathing operation and the air ventilation module 220 performs the breath function of the breathing operation.

The mesh tissue panel 300 is formed in a mesh lattice of a conductive material and mounted in the inner space of the case 100. In each grid line 301 formed of a conductive material, human or animal lung cells C are attached . One mesh structure panel 300 may be mounted in the space inside the case 100, but it is preferable that a plurality of the mesh structure panels 300 are mounted so as to have a structure similar to a lung structure of an actual human or animal.

The plurality of mesh tissue panels 300, 310, 320, 330, and 340 are formed to have lattice spaces d1, d2, d3, and d4 of different sizes as shown in FIGS. 4 and 5, A mesh structure panel 300 having a smaller mesh size along the inflow direction of the nanoparticles by the operation unit 200 is disposed in order.

In more detail, the mesh tissue panel 300 is formed in a mesh lattice form and the lung cells C are attached to the respective lattice lines 301, ) Can be uniformly cultured. The method of culturing and adhering cells as described above can be accomplished through various well-known cell culture methods, and a detailed description thereof will be omitted.

The plurality of mesh structure panels 300 are formed to have lattice spaces d1, d2, d3, and d4 of different sizes. The mesh structure panels 300 have meshes having a smaller mesh size along the inflow direction of the nanoparticles. And the tissue panel 300 are sequentially positioned.

That is, as described above, air and nano particles are introduced into the inner space of the case 100 from the outside by the particle supplying module 210 of the respiration actuating unit 200. At this time, As shown in FIG. 5, the mesh structure panels 300 having a smaller grid spacing are sequentially arranged. For example, as shown in FIG. 2 and FIG. 3, four mesh tissue panels 310, 320, 330 and 340 may be arranged in a row along the inflow direction of the nanoparticles. At this time, The mesh structure panel 310 located at a relatively large grid spacing d1 and then sequentially arranged at a grid spacing of d2, d3, and d4 toward the downstream can be arranged in a row.

When the air and the nanoparticles are introduced into the inner space of the case 100 by the breathing operation unit 200 according to this structure, the air and the nanoparticles form a plurality of mesh tissue panels 300 (310, 320, 330, and 340) And the flow passing sequentially. At this time, the relatively large amount of nanoparticles pass through the mesh structure panel 310 located at the uppermost position because the mesh structure panel 310 has a relatively large lattice spacing d1. However, the mesh structure panels 320, 330, d2, d3, and d4 are reduced, the amount of nanoparticles passing through the mesh structure panels 320, 330, and 340 is sequentially decreased.

That is, the nanoparticles introduced into the inner space of the case 100 by the breathing operation unit 200 are respectively supplied to the respective mesh tissue panels 300 (310, 320, 330, 340) in the course of passing through the plurality of mesh tissue panels 300 The nanoparticles pass through the plurality of mesh tissue panels 300: 310, 320, 330, and 340 in a manner that they are attached. Particularly, since the lattice spacing of the plurality of mesh tissue panels 300: 310, 320, 330, and 340 is sequentially decreased, the amount of nanoparticles passing through each of the mesh tissue panels 300, 310, 320, 330 and 340 is further reduced.

The nanoparticles relatively large in size are liable to adhere to the mesh structure panel 300 located on the upstream side because they are difficult to pass through the mesh structure panel 300. However, It is possible to reach and adhere to the mesh structure panel 300 located on the downstream side.

The arrangement structure of the mesh tissue panel 300 is similar to that of the lung structure. As described above, in the inspiration process during the breathing operation, the air is sucked into the organs, bronchi, bronchioles and alveoli, and the organs, bronchi, bronchioles and alveoli sequentially have a finer tissue structure. Likewise, the mesh structure panel 300 according to an embodiment of the present invention includes a mesh structure panel 300 having a smaller grid interval along the flow direction of nanoparticles.

According to this structure, the lung model device according to an embodiment of the present invention can have a structure similar to an actual lung structure, and in this structure, a breathing operation can be performed through the breathing operation unit 200, And is formed in a shape very similar to the function. That is, the lung model device according to an embodiment of the present invention has a structure similar to that of the lungs through the plurality of mesh tissue panels 300, and performs the breathing function of the lung through the breathing operation unit 200, It is formed in a shape very similar to the structure and function of the lung, and various types of tests can be performed using the same.

Particularly, since the lung tissue (C) is attached to the mesh tissue panel 300, the inhalation toxicity test for the nanoparticles is carried out in the absence of an experimental animal by a method of grasping the state change of the lung cell (C) can do. The state change of the lung cell C can be grasped by the electric signal measured by the signal detecting unit 20 in real time as described above.

As described above, for example, four mesh structure panels 300 may be provided. In the grid lines 301 of the mesh structure panels 300, the organs, bronchi, bronchioles, and alveolar cells are sequentially And the mesh tissue panel 300 may represent the organs, bronchi, bronchioles and alveoli tissues, respectively. When the mesh tissue panel 300 is installed in the inner space of the case 100 and the air and the nanoparticles are introduced into the inner space of the case 100 through the breathing operation unit 200, The lung tissue of the mesh tissue panel 300 may be damaged. An inhalation toxicity test for the nanoparticles can be performed in such a manner that the damage or active state of the lung tissue is inspected.

In addition, the lung tissue has a wide variety of tissues such as the alveolar tube and the alveolar parenchyma as well as the four tissues described above. For example, the lung tissue can be classified into 23 sub-tissues. The mesh structure panel 300 and the cell structure can be variously changed according to the user's need.

Next, the configuration of the breathing operation unit 200 performing the breathing function of the lung will be described in more detail.

The breathing operation unit 200 alternately repeats the inflow and outflow of air into the space inside the case 100. The particle supply module 210 for generating nanoparticles and introducing the air into the space inside the case 100 And an air discharging module 220 for discharging air from the inner space of the case 100. At this time, the particle supplying module 210 and the air discharging module 220 alternately operate repeatedly.

According to this structure, the nanoparticles are introduced into the inner space of the case 100 through the particle supply module 210, and after the air is introduced, the air is discharged through the air exhaust module 220. The nanoparticles may be discharged together with air discharged through the air exhaust module 220.

That is, the nanoparticles are introduced into the inner space of the case 100 together with the air through the particle supply module 210. During the inflow, the nanoparticles pass through the plurality of mesh tissue panels 300, Can be attached to each of the lung cells (C) attached to the plurality of mesh tissue panels 300 as described above. Since the nanoparticles are adhered to the lung cells C of the mesh tissue panel 300 during the inflow of the nanoparticles, the air is discharged through the air discharge module 220 to the lung cells C The attached nanoparticles remain attached to the lung cell C without being discharged. Of course, some nanoparticles that do not adhere to the lung cells (C) may be vented out together with air during the air discharge process.

Therefore, when the respiratory action unit 200 is operated, the nanoparticles repeatedly flow into the inner space of the case 100 and adhere to the lung cells C of the mesh tissue panel 300, so that when a long time elapses, The amount of nanoparticles adhering to the cell C is increased, and thus the lung cell C may be damaged or dead. Since the change in the characteristic of the lung cell C causes a change in the electrical resistance value of the mesh tissue panel 300, the electrical signal of the mesh tissue panel 300 is measured in real time through the signal detection unit 20, Evaluation of inhalation toxicity of nanoparticles on cell (C) can be performed in real time.

The particle supply module 210 includes a particle generator 211 for generating nanoparticles and a particle generator 211 for generating nanoparticles generated from the particle generator 211 to flow into the inner space of the case 100, And an air inflow pump 212 for introducing air into the main body 210. 5, the nanoparticles generated from the particle generator 211 are introduced into a separate mixing chamber 213 and mixed with the air by the air inflow pump 212 in the mixing chamber 213, (Not shown).

The air discharge module 220 includes a cushion bag 221 that is connected to the internal space of the case 100. The cushion bag 221 is connected to the inside of the case 100 through the particle supply module 210, The air introduced into the space can be introduced after passing through the plurality of mesh tissue panels 300.

6, air is introduced into the inner space of the case 100 from the upper layer, and the inflow air flows into the buffer bag 221 after passing through the plurality of mesh tissue panels 300. In other words, The bag (221) is mounted so as to communicate with the lower end of the case (100).

Therefore, the air and the nanoparticles introduced into the inner space of the case 100 by the particle supply module 210 always flow into the buffer bag 221 after passing through the mesh structure panel 300, The flow stably remains in the form of passing through all of the plurality of mesh tissue panels 300.

On the other hand, the buffer bag 221 is formed of an elastic material so that it can be restored in shape, and it can be formed as a kind of rubber balloon. 7, when the operation of the particle supply module 210 is completed and the inflow of air is stopped, the buffer bag 221 and the inner space of the case 100 are separated from each other by the elastic restoring force of the buffer bag 221 The air is discharged to the outside.

The air discharge module 220 may be configured to have a buffer bag 221 of elastic material, but an air discharge pump (not shown) for discharging air from the space inside the case 100 may be additionally provided . In this case, the buffer bag 221 need not be formed of an elastic material, but may be formed of a flexible material capable of simply changing the volume.

Since the buffer bag 221 is formed so as to communicate with the inner space of the case 100 and change its volume, the air and the nanoparticles are introduced into the inner space of the case 100 by the particle supply module 210 The pressure of the inner space of the case 100 can be prevented from rising and thus the function of keeping the inflow of air and nano particles smoothly by the particle supply module 210 is performed.

The main pipe 410 is installed in the case 100 so as to communicate with the internal space so that air can flow in and out of the internal space and the main pipe 410 is branched to the inflow pipe 411 and the discharge pipe 412 And the inflow pipe 411 is connected to the particle supply module 210 so that air and nano particles can be introduced into the space inside the case 100. The discharge pipe 412 discharges air from the space inside the case 100 So that the end is open.

5, a flow path switching valve 420 capable of selectively opening the inflow pipe 411 and the discharge pipe 412 is mounted on the branched portion of the main pipe 410, (420) is configured to operate in conjunction with the operating states of the particle supply module (210) and the air exhaust module (220). That is, the flow path switching valve 420 operates to open the inlet pipe 411 during the operation of the particle supply module 210 and operates to open the outlet pipe 412 during operation of the air outlet module 220 .

6, air and nano particles supplied from the particle supply module 210 are introduced into the inner space of the case 100 through the inflow pipe 411 and the main pipe 410, The plurality of mesh tissue panels 300 sequentially pass through the inner space of the bag 100 and then flow into the buffer bag 221. During the inflow of the air and the nanoparticles, the nanoparticles are attached to the lung cells C of the mesh tissue panel 300. When the operation of the particle supplying module 210 is completed, air is discharged from the space inside the case 100 by the elastic restoring force of the buffer bag 221 as shown in FIG. The air discharge path is discharged from the inner space of the case 100 through the main piping 410 and the discharge piping 412.

This process is repeatedly carried out to continuously inflow the nanoparticles into the inner space of the case 100 and then to examine changes in the characteristics of the lung cells C attached to the mesh tissue panel 300, The test can be performed.

The foregoing description is merely illustrative of the technical idea of the present invention and various changes and modifications may be made by those skilled in the art without departing from the essential characteristics of the present invention. Therefore, the embodiments disclosed in the present invention are intended to illustrate rather than limit the scope of the present invention, and the scope of the technical idea of the present invention is not limited by these embodiments. The scope of protection of the present invention should be construed according to the following claims, and all technical ideas within the scope of equivalents thereof should be construed as falling within the scope of the present invention.

10: closed model device 20: signal detection unit
30: Data processing unit 40: Output unit
50:
100: Case 200: Breathing operation unit
210: particle supply module 220: air discharge module
221: buffer bag 300: mesh tissue panel
301: Grid line 410: Main piping
411: Inflow pipe 412: Discharge pipe
420: flow path switching valve C: lung cell

Claims (9)

delete A respiration operation unit for introducing the nanoparticles together with air into the case internal space in such a manner that the inflow and outflow operations of the air to and from the case internal space are alternately repeated; A lung model device disposed in the case interior space and including a mesh tissue panel to which human or animal lung cells are attached to each grid line; And
A signal detection unit connected to the mesh tissue panel and detecting an electrical signal generated through the mesh tissue panel in real time,
/ RTI >
A plurality of mesh tissue panels are mounted inside the case,
Wherein the plurality of mesh tissue panels are formed to have lattice spacings of different sizes and mesh structured panels of a size smaller in lattice spacing are sequentially positioned along the flow direction of the nanoparticles in the case inner space,
The breathing operation unit
A particle supplying module for generating nanoparticles and introducing the nanoparticles into the internal space of the case together with air; And
An air discharge module for discharging air from an inner space of the case,
Wherein the particle supply module and the air discharge module alternately and repeatedly operate.
3. The method of claim 2,
A data processing unit for receiving the electrical signal detected by the signal detecting unit and determining a damaged state of the lung cell; And
An output unit for outputting a determination result of the data processing unit;
Further comprising: an inhalation toxicity measuring device for measuring an inhalation toxicity of the lung model;
The method of claim 3,
Wherein the data processing unit compares an electric signal applied from the signal detection unit with a separate database data to determine the damage state of the lung cell.
5. The method of claim 4,
Wherein the database data is formed by databaseing damage states of lung cells according to electrical signals generated through the mesh tissue panel.
delete 3. The method of claim 2,
The air exhaust module
And a plurality of mesh tissue panels disposed in the case to communicate with the inner space of the case through the plurality of mesh tissue panels,
Wherein the buffer bag is made of an elastic material so that the shape of the buffer bag can be restored, and air introduced into the case inner space is discharged from the inner space of the case by elastic restoring force of the buffer bag. Real - time inhalation toxicity testing device.
3. The method of claim 2,
The main pipe is installed in the case so as to communicate with the inner space so that air can flow in and out of the inner space,
Wherein the main pipe is branched into an inlet pipe and an outlet pipe, and the inlet pipe is connected to the particle supply module so that air and nano particles can be introduced into the case internal space, And the open end is opened so that it can be discharged.
6. The method according to any one of claims 2 to 5,
The mesh tissue panel
Wherein each of the lattice lines is formed in such a manner that human or animal lung cells are uniformly cultured.

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