KR101580644B1 - Analysis apparatus for flowing blood - Google Patents

Abstract

A flowing blood measuring device of the present invention is capable of measuring viscosity and a coagulation property of red blood cells in flowing blood. The device includes: a plate accommodating a body of an organism; a blood inflow part receiving blood, discharged from the artery of the organism; a pulsation removing part connected to the blood inflow part, and discharging the blood by removing pulsation from the blood; a micro-fluid chip connected to the pulsation removing part to measure the viscosity of the blood, discharged from the pulsation removing part; a blood moving part connected to the micro-fluid chip, and delivering the blood, flowing into the flowing blood measuring device, to the vein of the organism; and an ultrasonic image part placed in a part of the blood moving part, and applying an ultrasonic wave to the blood, flowing in the blood moving part, to convert the ultrasonic wave, scattered by red blood cells, into visual information. Therefore, the present invention is capable of measuring a variety of hemorheologic properties such as viscosity and coagulation properties at the same time.

Description

[0001] ANALYSIS APPARATUS FOR FLOWING BLOOD [0002]

The present invention relates to a flow blood measuring apparatus capable of measuring hemoglobinological information such as aggregation of erythrocytes and viscosity of blood from a blood in a fluid state.

The mortality rate due to cardiovascular disease is very high worldwide. Hypertension, stress, obesity and smoking have been reported as the main cause of clinical outcome. However, information on the causes of these causes does not sufficiently explain the mechanism of cardiovascular disease. In order to elucidate these in more detail, rheological information of blood is needed in addition to information on blood flow.

Depending on the type of cardiovascular disease, blood rheological properties such as erythrocyte aggregation, blood viscosity and viscoelasticity are altered, and the relationship between these rheological properties and cardiovascular disease continues to be studied.

Conventional devices used to measure hemorheological information are mostly measured under couette flow conditions. However, because the actual blood flows through the blood vessels, it may be different from the characteristics measured in the quet flow. In order to measure blood rheological information, the blood which has been treated with anti-coagulation treatment in the blood also changes into red blood cells as time elapses. As a result, rheological properties such as aggregation of red blood cells and blood viscosity are changed. Because of this, biologic characteristics are changed according to the flow conditions, so there is a limit to existing measurement methods using collected blood.

The present invention relates to a flow blood measurement apparatus proposed for simultaneous measurement of blood flow tie point and erythrocyte flocculation characteristics in order to diagnose a cardiovascular disease or monitor progress.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are not intended to limit the invention to the precise form disclosed. It can be understood.

The apparatus for measuring a flow blood according to an embodiment of the present invention can measure the viscosity from blood in a fluid state and the aggregation characteristics of red blood cells present in the blood and can measure a plate on which a body of a living organism is placed, And a blood inflow part connected to the blood inflow part and connected to the pulsation removing part for removing the pulsation present in the blood to discharge the blood, A microfluidic chip for measuring the viscosity of the blood, a blood transfer part connected to the microfluidic chip, for transferring the blood introduced into the flow blood measurement device to the vein of the living organism, , Ultrasonic waves are applied to the blood moving in the blood moving part, and ultrasonic waves scattered by the red blood cells And an ultrasound image part for converting the sound wave into the time information.

The microfluidic chip may include a first channel through which the blood flows, a second channel through which the buffer flows, and a bridge channel connecting the first channel and the second channel.

The ultrasound imaging unit includes an ultrasound transducer for generating ultrasound to be applied to the blood, an ultrasound signal collector for collecting ultrasound signals scattered by the red blood cells, and an ultrasound image display unit for converting the collected ultrasound signals into time information. . ≪ / RTI >

The plate of the present embodiment may include a heating pad for heating the plate and a temperature measuring unit provided on the plate for measuring the body temperature of the living organism. In order to measure the electrocardiogram of the living organism, And a measurement unit.

In addition, the apparatus for measuring flow blood of the present embodiment may further include an auxiliary pump for controlling the flow rate of the blood.

According to the present invention, there is provided a flow blood measuring apparatus capable of measuring various rheological properties by simultaneously measuring viscosity and erythrocyte agglutination characteristics under the flow of blood. At this time, it is possible to acquire information on various indicators such as cancer cells to be transferred, diabetes mellitus and malaria examination by variously adjusting the flow conditions of the blood.

More specifically, first, according to the present invention, since the hemodynamic properties are measured from the actual blood flowing, the viscosity and the change of the erythrocyte agglutination characteristics due to the change of the shear strain (Γ) are simultaneously measured can do.

Second, when the present invention is applied to an extracorporeal circulation conduit that connects a vein and an artery of a mouse, an animal, or a human being to the outside, the blood rheological properties can be directly measured without denaturation of the blood.

Third, by providing an auxiliary pump in the apparatus for measuring blood flow according to the present invention, it is possible to measure changes in hemodynamic characteristics under various flow conditions.

Fourth, the apparatus for measuring blood flow according to the present invention can measure blood glucose using a biochip in addition to the viscosity of blood and the coagulation characteristics of blood cells, and when additional microchannels are added, blood coagulation or deformability can be measured together. Among the measured physical properties, blood viscosity, coagulation characteristics of red blood cells, blood glucose and blood cell deformity are used for cardiovascular disease or diabetes screening, and hematological deposition or deformity information can be used for malaria screening.

1 is a view showing a flow blood measuring apparatus according to an embodiment of the present invention.
2 is a view illustrating a microfluidic chip according to an embodiment of the present invention.
FIG. 3 is a view illustrating the measurement of blood viscosity by a microfluidic chip including a first channel, a second channel, and a bridge channel according to an embodiment of the present invention. Referring to FIG.
4 is a view showing a state where red blood cells are agglomerated to form an aggregate.
5 is a view illustrating an image taken by the ultrasound imaging unit according to an embodiment of the present invention.
6 is a graph showing that the rheological factors of the blood can be changed over time.
FIG. 7 is a graph showing changes in viscosity and degree of aggregation of erythrocytes according to the shear strain. FIG.
8 is a graph showing the correlation between the tie point of blood and the degree of coagulation of erythrocytes.
FIG. 9 is a conceptual diagram for separating aggregates of erythrocytes and showing changes in degree of aggregate separation according to shear strain. FIG.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the following description of the present invention, the well-known functions or constructions are not described in order to simplify the gist of the present invention.

FIG. 1 shows a flow blood measurement apparatus 100 according to an embodiment of the present invention. The apparatus 100 for measuring a flow blood according to an embodiment of the present invention shown in FIG. 1 is a device capable of measuring the viscosity of blood from the blood in a fluid state and the aggregation characteristics of red blood cells present in the blood.

1, the apparatus for measuring blood flow 100 according to the present embodiment includes a plate 110, a blood inflow section 120, a pulsation removing section 130, a microfluidic chip 140, An ultrasound imaging unit 150, and an ultrasound imaging unit 160.

In the plate 110 according to this embodiment, the body of the living organism for supplying the blood to be measured by the flow blood measurement apparatus 100 is disposed.

As shown in FIG. 1, the organism according to the present embodiment may be an animal such as a rat, but is not limited thereto. It may be a variety of organisms such as mammals, reptiles, amphibians including humans, Lt; / RTI >

Therefore, the apparatus 100 for measuring a flow blood of the present invention measures the viscosity of blood and the aggregation characteristics of erythrocytes from the blood in a fluid state supplied from living animal living bodies including humans, and then delivers the blood to the living body.

The blood inflow section 120 is connected to the artery of the living organism disposed on the plate 110 and introduces the blood discharged from the artery into the flow blood measurement apparatus 100 according to the present embodiment.

The blood inflow section 120 according to the present embodiment includes a needlelike needle in direct contact with an artery of a living organism to receive blood, a blood transfer tube for moving blood from living organisms to the flow blood measurement apparatus 100, But the present invention is not limited thereto and may be included in the scope of the present invention if it is a conventional configuration in which blood can be supplied from the artery of the living organism and introduced into the apparatus of the present embodiment.

The pulsation removing part 130 is connected to the blood inflow part 120 and removes the pulsation existing in the blood. Since the blood flowing from the blood inflow section 120 is arterial blood discharged from the heart of living organism, it has a pulsation according to the heartbeat of the living organism. On the other hand, since the blood flow measuring apparatus 100 of the present embodiment requires blood to be supplied at a constant flow rate, pulsations present in the blood must be removed through the pulsation removing unit 130. When the pulsation existing in the blood is removed by the pulsation removing unit 130, the flow rate of the blood discharged from the pulsation removing unit 130 becomes constant.

The pulsation eliminator 130 according to the present embodiment may include a chamber 132 and a pressure holding unit 134. The chamber 132 provides an enclosed space therein, and provides a space in which the inflow blood can be temporarily stored. The pressure holding unit 134 can expand and contract in volume and compress or expand the air in the chamber 132 to maintain the pressure in the chamber 132 constant when the pressure of the blood flow changes with the pulsation.

The pressure in the chamber 132 can be kept constant by the pressure holding unit 134 and the flow rate of the blood discharged from the pulsation eliminator 130 is eliminated by eliminating the pulsation existing in the blood.

The microfluidic chip 140 measures the viscosity of the blood by measuring the flow rate ratio between the buffer and the blood which is already known viscosity. FIG. 2 shows a microfluidic chip according to the present embodiment.

2, the microfluidic chip 140 according to the present embodiment includes a first channel 142, a second channel 144, and a bridge channel 146 for measuring a flow rate ratio between the buffer solution and blood ).

Blood for viscosity measurement flows into the first channel 142 and is passed through the second channel 144, and a buffer solution already known in viscosity flows into the second channel 144. The first channel 142 and the second channel 144 are connected to the bridge channel 146 and the blood of the first channel 142 and the buffer of the second channel 144 are introduced into the bridge channel 146 Or may be discharged.

3 is a view illustrating measurement of the viscosity of blood by the microfluidic chip 140 including the first channel 142, the second channel 144, and the bridge channel 146 according to the present embodiment.

3, the first channel 142 and the second channel 144 according to the present embodiment are formed in the same shape, and the first channel 142 and the second channel 144 are formed on the basis of the bridge channel 146, The channels 144 may be symmetrically arranged. At this time, the buffer solution passing through the second channel 144 according to the present embodiment may be phosphate buffered saline.

Blood and buffers passing through the first channel 142 and the second channel 144, which are formed in the same shape and are symmetrically arranged, may flow into the bridge channel 146, respectively, The pressure ratio of the liquid can be calculated as the viscosity ratio.

3, when the flow rate of the buffer solution is increased (e), the flow rate of the blood flowing into the bridge channel 146 is decreased. Conversely, when the flow rate of the buffer solution is decreased (a, b, c, the flow rate of the blood flowing into the bridge channel 146 is increased. Therefore, controlling the flow rate of the buffer solution can form an equilibrium state (d) in which the buffer solution and the blood no longer flow into the bridge channel 146. By measuring the flow ratio of the buffer to the blood in the equilibrium state, the viscosity of the blood can be measured using a buffer solution already known in viscosity.

Referring to FIG. 2, the formula for measuring the viscosity of blood is as follows.

[Equation 1]

(P _Blood : the pressure of the red point in the first channel 142 through which the blood flows

Q _Blood : Flow rate of blood flowing through the first channel (142)

R _L : flow resistance of the first channel 142

P _PBS : Pressure at the blue point in the second channel 144 through which the buffer flows

Q _PBS : the flow rate of the buffer solution flowing in the second channel 144 (fluctuation depending on the flow condition)

R _R : flow resistance of the second channel 144

P _B : Pressure when the pressure is in the same balancing state inside the symmetrical first channel 142 and the second channel 144

Q _Blood ^B : When in equilibrium, the pressure of the red point in the first channel (142)

Q _PBS ^B : When in an equilibrium state, the pressure of the blue point in the second channel 144 through which the buffer flows

μ _Blood : The viscosity of blood

μ _PBS : viscosity of the buffer)

Meanwhile, the microfluidic chip 140 according to the present embodiment may further include a syringe pump 148. The syringe pump 148 according to this embodiment is disposed at one end of the second channel 144 to supply the buffer solution and control the flow rate of the buffer solution. By more accurately controlling the flow rate of the buffer solution by the syringe pump 148, the viscosity of the blood measured therefrom can also be measured more accurately.

The blood moving part 150 is connected to the microfluid chip 140 and the vein of the living organism, and transfers the blood discharged from the microfluidic chip 140 to the vein of the living organism.

The blood moving part 150 according to the present embodiment is provided with a needlelike needle and a flow blood measurement device 100 so as to be able to directly communicate with the vein of the living organism and deliver the blood to the vein as in the blood inflow part 120 But the present invention is not limited thereto and can be included in the scope of the present invention if it is a conventional configuration that allows blood to be transferred to the veins of living organisms.

The ultrasound imaging unit 160 is disposed in a part of the blood moving unit 150 and applies ultrasonic waves to the blood moving in the blood moving unit 150. The applied ultrasound is scattered by red blood cells in the blood, and the ultrasound imaging unit 160 of the present embodiment collects ultrasound scattered and converts it into visual information.

The ultrasound imaging unit 160 may include an ultrasound transducer 162, an ultrasound signal collector 164, and an ultrasound image display unit 166.

The ultrasonic transducer 162 generates ultrasonic waves to be applied to blood, and the ultrasonic signal collector 164 receives ultrasonic signals scattered by red blood cells. The ultrasound image display unit 166 converts the ultrasound signals collected by the ultrasound signal collector 164 into images that are time information and displays them on the display.

FIG. 4 shows a state where red blood cells are agglomerated to form an aggregate, and FIG. 5 shows an image taken by the ultrasound imaging unit 160 according to the present embodiment.

As shown in FIG. 4, when the red blood cells form an aggregate, the applied ultrasound is scattered by the aggregates. As the number of erythrocyte aggregates increases, the amount of scattered ultrasound signals increases.

Accordingly, as shown in FIG. 5, the brightness of the screen displayed on the ultrasound image display unit 166 also becomes darker as the amount of ultrasound signals scattered by the erythrocyte agglomerates increases. Therefore, the degree of agglutination of red blood cells can be measured through the brightness of the screen displayed on the ultrasound image display unit 166. This will be described in more detail with reference to Figs. 7 to 9 below.

Table 1 shows changes in blood rheological parameters, such as blood viscosity and erythrocyte aggregation and degree of aggregate separation, over time after blood collection.

0 h 0.5 h 1 h 2 h 3 h Viscosity (cP) 6.4 ± 0.2 7.4 ± 0.2 8.2 ± 0.1 11.0 ± 0.3 11.8 ± 0.1 Erythrocyte aggregation (dB) 20.09 ± 0.15 20.28 ± 0.13 20.42 + 0.13 20.94 + 0.23 21.14 ± 0.11 Aggregate separation _s 0.0644 ± 0.006 0.0551 0.005 0.0503 0.003 0.0481 0.005 0.04423 ± 0.004

In Figure 6, it is shown that the rheological factors of the blood can change over time. 6, Q _PBS is the flow rate of the buffer solution when the inside of the bridge channel 146 is in an equilibrium state, Q _Blood is the flow rate of blood when the inside of the bridge channel 146 is in equilibrium, And is measured using the video unit 160. FIG. Dextran injection is an artificially injected drug to make red blood cell aggregation stronger during the experiment.

T is the body temperature of the creature, the creature providing flow blood according to this embodiment, and HR is the heart rate of the mouse. μ is the viscosity of the blood in the rat, and P is the mean blood pressure in the abdominal aorta of the rat. E is an ultrasound signal of the blood circulation part measured by the ultrasound imaging part 160 and represents the degree of red cell aggregation, and D _s is the degree of separation of the aggregate, which indicates the degree of collapse of erythrocyte aggregation.

6 (a) is a schematic view of the apparatus for measuring blood flow 100 according to the present embodiment. FIG. 6 (b) is a view showing a state where the inside of the microfluidic chip 140 is fluidically balanced And a flow rate of the blood. In this case, the flow rate of blood was measured by using ultrasonic measurement technique and it was measured without additional blood collection. In order to give an abrupt hemorrhodic change, the drug (Dextran) was injected in 50 minutes to increase viscosity and erythrocyte aggregation It is. FIG. 6 (c) is a graph showing changes in body temperature and heart rate with time, and FIG. 6 (d) shows changes in viscosity of blood and mean blood pressure in the abdominal aorta according to time Graph. FIG. 6 (e) is a graph showing changes in ultrasound signals and degree of separation of aggregates indicating the extent of erythrocyte aggregation with time.

Referring to Table 1 and FIG. 6, when the blood is collected to measure the rheological information of the blood, the rheological parameters of the blood are changed with time, which makes it difficult to accurately measure the blood.

However, the ultrasound imaging unit 160 according to the present embodiment measures the aggregation characteristics of red blood cells by applying ultrasonic waves to the blood that is disposed and moved in a part of the blood movement unit 150. Therefore, it is not necessary to separately extract blood, and therefore it is possible to measure more accurately the measurement of the coagulation characteristic of erythrocytes from the blood in the fluid state, without elapsing the time required for the measurement. In addition, the viscosity can be measured in the microfluidic chip 140 having a relatively high shear strain, and the change in the hemodynamic properties can be easily determined by measuring the erythrocyte aggregation at a low shear strain.

With respect to how the hemodynamic information can be calculated through the measurement results of the flow blood measurement apparatus 100 according to the present embodiment, the correlation between the degree of aggregation of red blood cells, the viscosity of blood, and the separation of aggregates of erythrocytes Will be described in detail below with reference to FIGS. 7 to 9. FIG.

FIG. 7 shows changes in viscosity and degree of coagulation of erythrocytes according to the shear strain, and FIG. 8 shows the correlation between the blood tie point and the degree of aggregation of erythrocytes. FIG. 9 is a conceptual diagram of the separation of aggregates of erythrocytes and a change in degree of aggregate separation according to shear strain.

More specifically, FIG. 7 shows the change of the viscosity (μ) and the average internal ultrasound signal value (E) according to the change of the shear strain (Γ). As described above, since the ultrasound signal scattered from the blood becomes stronger as the erythrocyte aggregation increases, the degree of erythrocyte aggregation can be estimated by using the ultrasonic signal.

The graph of FIG. 7 was measured using the blood of rats according to one embodiment of the present invention. In order to observe the changes in viscosity and coagulation characteristics under various hemodynamic conditions, blood was added to phosphate buffered saline to prevent erythrocyte aggregation (PBS), a Dextran-treated sample that promotes erythrocyte aggregation, and a DIDS-treated sample in which erythrocyte aggregation is inhibited.

Figure 8 shows the relationship between viscosity and ultrasonic signals under various conditions. The correlation coefficient (R) between the two factors was found to be highly correlated. The experimental results are fitted to the solid line, and the dashed line and dotted line represent the 95% confidence interval and the predicted interval. It can be seen that viscosity and erythrocyte aggregation show a linear relationship under various conditions.

FIG. 9 is a conceptual diagram for separating erythrocyte aggregates and shows the change in degree of aggregate separation according to shear strain. As shown in the conceptual diagram of Fig. 9, erythrocyte aggregates of flow blood are separated into small aggregates or individual red blood cells by shear stress. The dissociation of blood speckle (D _s ) can be estimated from the ultrasound signals scattered from the blood using the following equation.

&Quot; (2) "

Where m _t1 and m _t2 are the mean values of image brightness of tile 1 or 2, σ _t1 , σ _t2 are the standard deviations of each tile, and σ _tlt2 is the covariance of the two tiles. Tile 1 is a part of the ultrasound image obtained at a specific moment (t), and tile 2 is a part of the ultrasound image acquired at a certain time (t). The distance traveled by the blood is inferred and the tile 2 is moved accordingly. According to FIG. 9, as the shear strain increases, the degree of dispersion of the aggregate increases sharply. Using the measured flow rate and erythrocyte aggregation information, other rheological factors such as segregation of aggregates can be measured. At this time, the viscosity of the blood is measured at high shear strain conditions and the erythrocyte agglomeration is measured at low shear strain.

The flow blood measurement apparatus 100 according to the present embodiment is capable of measuring the blood rheological information to be measured under different shear strain conditions such as a high shear strain (separation phenomenon of erythrocyte aggregates) and a low shear strain Can be measured at once from the blood in the fluid state.

The apparatus for measuring blood flow 100 according to the present embodiment may further include a heating pad 112 for heating the plate 110 so that the body temperature of the living organism disposed on the plate 110 can be maintained constant .

If the body temperature of the organism is maintained within a certain range, the hematological characteristics of the blood can be measured more accurately without affecting the temperature and the hematological characteristics of the blood. Therefore, the apparatus 100 for measuring flow blood according to the present embodiment may further include a heating pad 112 to compensate for a decrease in body temperature caused when blood circulates out of the living organism, thereby maintaining a constant body temperature of the living organism have.

The flow blood measurement apparatus 100 according to the present embodiment may further include a temperature measurement unit 114 for measuring the body temperature of the living organism to check whether the temperature of the living organism is kept constant. The temperature measuring unit 114 according to the present embodiment may be provided on the plate 110 so as to measure the body temperature of a living body disposed on the plate 110.

Similarly, the flow blood measurement apparatus 100 according to the present embodiment may further include an electrocardiogram measuring unit 116 for measuring an electrocardiogram of a living organism. Since the electrocardiogram measuring unit 116 according to the present embodiment is also for measuring the electrocardiogram of a living organism, it may be provided on the plate 110 on which the living organism is disposed.

The flow blood measurement apparatus 100 including the temperature measurement unit 114 or the electrocardiogram measurement unit 116 may further include a display unit 170 capable of visualizing and displaying information on body temperature or electrocardiogram .

The display unit 170 converts electrical signals relating to body temperature or ECG measured by the temperature measuring unit 114 or the electrocardiogram measuring unit 116 into time information and displays the time information so that the time information can be confirmed from the outside.

In addition, the apparatus 100 for measuring flow blood according to the present embodiment further includes an auxiliary pump (not shown) which can more accurately and variously change the flow rate of blood.

The auxiliary pump (not shown) can change the flow rate of the blood or the shear strain thereof, so that various hematological characteristics can be measured. In addition, various characteristics such as blood cell deformity, sedimentation rate, blood glucose, etc. measured from flowing blood can be applied to the study of biomedical indicators.

The apparatus for measuring blood flow 100 according to an embodiment of the present invention has been described above. According to the present invention, the viscosity of the blood and the erythrocyte agglutination characteristics can be measured in the flow conditions of the blood. That is, hemodynamic information can be measured without extracting blood separately.

According to the apparatus 100 for measuring a flow blood of the present invention, the apparatus 100 for measuring a flow blood of the present invention can measure blood cohesion coefficient and erythrocyte flocculation characteristics of blood flowing from the inside of a living body, Can be measured. Therefore, it is possible to measure in real time the change of the blood rheological properties such as the viscosity of the blood and the degree of agglutination of erythrocytes from the blood in the fluid state in which denaturation does not occur over time due to the extraction and extraction of the blood. The measured hemorrhagic characteristics can be used for the diagnosis of cardiovascular diseases or hematologic diseases of living organisms.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It is obvious to those who have. Accordingly, it should be understood that such modifications or alterations should not be understood individually from the technical spirit and viewpoint of the present invention, and that modified embodiments fall within the scope of the claims of the present invention.

100: flow blood measuring device
110: Plate
112: heating pad
114: Temperature measuring unit
116: electrocardiogram measuring unit
120: blood inflow section
130: Pulse removal
132: chamber
134: Pressure maintaining unit
140: Microfluidic chip
142: First channel
144: Second channel
146: Bridge Channel
148: Syringe pump
150:
160: Ultrasonic image part
162: Ultrasonic transducer
164: Ultrasonic Signal Collector
166: Ultrasound image display unit
170:

Claims (6)

A flow blood measurement apparatus capable of measuring viscosity from a blood in a fluid state and coagulation characteristics of red blood cells present in the blood,
Plates on which the body of the organism is placed;
A blood inflow portion for introducing the blood discharged from an artery of the living organism;
A pulsation removing part connected to the blood inflow part and discharging the blood by removing pulsation existing in the blood;
A microfluidic chip connected to the pulsation removing unit and measuring a viscosity of the blood discharged from the pulsation removing unit;
A blood moving part connected to the microfluidic chip and transferring the blood introduced into the flow blood measurement device to the vein of the living organism; And
And an ultrasonic imaging unit disposed in a part of the blood moving unit and applying ultrasound to the blood moved in the blood moving unit to convert ultrasound scattered by the red blood cells into time information. The method according to claim 1,
The microfluidic chip may include:
A first channel through which the blood flows;
A second channel through which the buffer solution flows; And
And a bridge channel connecting the first channel and the second channel. The method according to claim 1,
The ultrasound imaging unit includes:
An ultrasonic transducer for generating ultrasonic waves to be applied to the blood;
An ultrasound signal collector for collecting ultrasound signals scattered by the red blood cells; And
And an ultrasound image display unit for converting the collected ultrasound signals into time information. The method according to claim 1,
The plate may comprise:
A heating pad for heating the plate; And
And a temperature measurement unit for measuring a temperature of the living body. The method according to claim 1,
The plate may comprise:
Further comprising an electrocardiogram measuring unit for measuring an electrocardiogram of the living organism. The method according to claim 1,
Further comprising an auxiliary pump for regulating the flow rate of the blood.

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