KR101557894B1 - Litsea japonica of oil as an active ingredient of lightening composition for improving and Method of Manufacture - Google Patents

Abstract

The present invention relates to a composition for whitening improvement (whitening cosmetic) containing an oil obtained from a crow tree, as an active ingredient, and a process for producing the same.
The whitening cosmetic composition of the present invention (whitening cosmetic) contains oil extracted from the crow's side tree as an active ingredient, and the oil of the crow's side tree is obtained from the fruit of the crow's side tree or pure fruit or seed Or the oil of the crow tree is seed oil obtained as a seed oil of a crow tree and fruit oil obtained as a pure fruit of a crow tree, and the above-mentioned fruit oil and seed oil are used singly or in combination.
A method for producing an oil used as an effective ingredient of a composition for whitening improvement (whitening cosmetic) of the present invention is divided into pure fruit and seed of a crow tree, and the pure fruit or seed is weighed as a solvent, n-hexane And the mixture was stirred at room temperature for 24 hours to extract and the hexane layer was removed.

Description

TECHNICAL FIELD The present invention relates to a composition for improving whiteness and a method for preparing the same,

The present invention relates to a composition for whitening improvement (whitening cosmetic) containing an oil obtained from a crow tree, as an active ingredient, and a process for producing the same.

Litsea japonica used in the present invention is an evergreen tree species and is distributed vertically in the beaches and at the foot of mountains within 700 m above sea level.

Previous studies on the chemical composition of this plant have reported several types of essential oils, fatty acids, lactones, alkaloids and terpenoids.

Hamabiwalactone A, hamabiwalatone B, akolactone B, litsealactone A and litsealactone B, which are known as Lactones, were isolated.

In addition, the anti-complement activity of epicatechin, afzelin, quercitrin and tiliroside, which are flavonoids isolated from leaves, has been investigated, and tiliroside has been reported to exhibit the strongest inhibitory action on the complement system.

(DPPH, Xanthine Oxidase, Superoxide radical -> Supernox radical -> Supernox radical scavenging activity in the fraction EtOAC layer) and LDH, NO, PGE ₂ (impurities) in fractions of 80% (Epicatechin, Afzlin, Quercitrin, Tiliroside, and Pyridyl) by using silica column, and measuring the amount of flavonoids in leaves (Epicatechin, Afzlin, Quercitrin, Tiliroside, The results of this study were as follows: 1) Anti-complement system activity was measured after Myricitrin, Kaempferol, Quercetin, Myricetin, and PHYTOTHERAPY RESEARCH, In the study, the activities of the crows were 10 ^-1 mg / mL (in the case of the crow tree) And the inhibitory effect was about 18%.

In addition, in a study on the induction effect of HL-60 leukemia cell apoptosis in a crow tree (Litsea japonica) (2009, Kim, Elvira et al., Ykhak Hoeji, 53 (1) HL-60 (cytotoxicity) -MTT measurement showed that the cells were not toxic to normal cells and showed HL-60 (leukemia cell) cell proliferation inhibition effect, DNA fragmentation analysis, Western blot analysis The expression of Bcl-2 and the activation of caspase induced apoptosis and inhibited the proliferation of Bcl-2.

The prior art of the patent literature includes a technology for the composition for the prevention and treatment of cancer diseases containing as an active ingredient a root extract of crows in Korean Patent No. KR-10-2009-0091477 (published on 2009.08.28) ) A technology relating to a composition for the prevention and treatment of diabetic complications, which comprises the extract of Raven or the fraction thereof as an active ingredient in the patent No. 10-1243243 (filed on Mar. 07, 2012), is provided.

(KR) Patent Publication 10-2009-0091477 (Publication date 2009.08.28) (KR) Patent Registration No. 10-1243243 (registered on March 7, 2012)

The present invention provides a composition for whitening improvement (whitening cosmetic) containing the oil of a crow tree having a whitening effect as an active ingredient and a manufacturing method thereof.

That is, in order to develop a whitening effect material from a natural product, the present applicant found out that the oil of the crow tree (fruit or seed) was excellent in whitening effect while screening from a natural product, (Whitening cosmetic) and a process for producing the same.

The composition for whitening improvement (whitening cosmetic) of the present invention contains an oil extracted from a crow tree as an active ingredient.

The oil of the crow tree is obtained from either the fruit of the crow tree (excluding seeds) or the fruit including seeds or seeds,

Or the oil of the crow tree is a fruit oil obtained by the fruit of a crow tree (excluding seeds) and a seed oil obtained by a seed of a crow tree, and the above-mentioned fruit oil and seed oil are used singly or in combination.

The oil of the crow tree may be extracted in various ways. In the present invention, n-hexane is used as a solvent, and the mixture is stirred to extract hexane.

Another method for producing an oil used as an active ingredient of a whitening improving composition (whitening cosmetic), which is another inventive method, comprises the steps of: dividing the fruit of a crow tree (excluding seeds) and seeds, (n-hexane) was added in an amount of 9 to 11 times by weight, the mixture was stirred at room temperature for 24 hours, and the hexane layer was removed.

The oil of the crow tree of the present invention contains Beta-cubebene (22.96%), Beta-selinene (11.23%), Gamma-Elemene (10.21%) and Beta-Caryophyllene (7.37% Ability to inhibit synthesis of melanin in cells, and thus has an effect of improving whitening, so that it can be effectively used as an effective ingredient of a composition for whitening improvement (whitening cosmetic).

1 is a graph showing the inhibitory activity of tyrosinase activity of fruit oil and seed oil of a crow tree according to the present invention.
FIG. 2 is a graph showing the inhibitory effect on intracellular melanin synthesis of fruit oil and seed oil of a crow tree according to the present invention.
Figure 3 is a chromatogram of fruit oil of a crow tree according to the present invention.

It is to be understood that the terms and words used in the present specification and claims should not be construed as limited to a conventional or dictionary sense and that the configurations shown in the embodiments and drawings described herein are only the most preferred embodiments of the present invention It is to be understood that the invention is not limited to the specific embodiments disclosed herein, and that various equivalents and modifications may be made thereto without departing from the scope of the present invention.

The present invention provides a whitening cosmetic (whitening-specific composition) material containing an oil of a crow tree as an active ingredient.

Hereinafter, the present invention will be described in detail. Row tree fruit is called 'pure fruit', and seeds in the fruit are classified as 'seeds'.

The crude tree oil, which is an active ingredient of the composition according to the present invention, is separated into pure fruit (excluding seeds) or seeds (seeds in the fruit), and oil is obtained from them.

However, it is also possible to acquire oil from the fruit of the crow tree (without seed separation, ie, pure fruit and seed mixed).

In the embodiment of the present invention, in order to utilize various compositions such as medicines, foods, cosmetics, etc., it is necessary to separate the fruits and seeds from each other so as to obtain oil therefrom. In this case, oil is obtained from fruits including seeds Which is also within the scope of the present invention.

In addition to the extraction method using n-hexane, which is an embodiment of the present invention, extraction methods such as supercritical extraction method, distillation method, and pressing method can be used as a method of extracting the fruit of the crow tree, the pure fruit, It is possible to obtain oil by using. However, when the distillation method and the pressing method are used, the amount of oil to be extracted has a disadvantage less than that of the extraction method using the n-hexane of the embodiment.

- Nine hexane (n-hexane), which is a solvent, is added to 9-11 times (preferably 10 times) the weight of the seeds of the pure fruit of the crow tree, 15 to 25 ° C) for 24 hours, and then the hexane layer is removed to obtain a crow's noodle oil.

- Nine hexane (n-hexane) is added to the oil of the seeds of the tree in the crow's side and dried seeds (Litsea japonica) of the crows, and 9-11 times (preferably 10 times) ) For 24 hours, and then the hexane layer is removed to obtain a tree seed oil for crows.

The corn tree pure fruit oil and the seed oil may be used alone or in a mixed ratio.

The crude oil of the corn root of the present invention and the seed oil showed excellent efficacy as a result of the inhibition of tyrosinase activity.

In addition, the amount of synthesis of intracellular melanin was measured.

Accordingly, the pure white fruit of the crow and the oil of the seed according to the present invention can be used as a cosmetic (composition for whitening characteristics) useful for improving whitening.

Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are provided only for the purpose of easy understanding of the present invention, and the present invention is not limited by the examples.

Example 1 : Production of pure fruit oil of a crow tree

5,000 ml of n-hexane was added to 500 g of a lysoya japonica pure lyophilus, and the mixture was stirred at room temperature (20 ° C) for 24 hours. Then, hexane was removed to remove oil 95g.

Example 2  : Oil production of seed part of nuts tree fruit of crow

5,000 ml of n-hexane was added to 500 g of freeze-dried Litsea japonica seeds, and the mixture was stirred at room temperature (20 ° C.) for 24 hours. Then hexane was removed to obtain 46 g of oil .

Experimental Example 1 : Analysis of components of pure fruit oil of a crow tree

The analysis of the components of the pure wood oil of crows obtained according to Example 1 was carried out.

Experimental method: Instrument Shimadzu, it was used QP2010Plus, Column is RTX-5 (30m x 0.25mm, 0.25㎛) a was used, with other conditions Injection temperature 250 ℃, Detector temperature 250 ℃, Mass range (m / z ) 30-400, and injection volume 1 μL.

Experimental Results: The crude oil obtained from Example 1 was analyzed to obtain the results as shown in Table 3 and Table 1. As the result, Beta-cubebene (22.96%), Beta-selinene (11.23%), , Gamma-Elemene (10.21%) and Beta-Caryophyllene (7.37%).

Table 1 below is an analytical table of pure white fruit oil obtained according to Example 1.

Compound Name Area (%) peak Remarks beta-cubebene 22.96 14 beta-selinene 11.23 15 Gamma-Elemene 10.21 16 beta-Caryophyllene 7.37 8

Experimental Example 2 : Whitening improvement effect of pure fruit or seed oil of crow tree (1): Tyrosinase inhibitory ability

In order to confirm the whitening improvement effect of pure white fruit oil and seed oil obtained according to Example 1 and Example 2, an evaluation test for inhibition of tyrosinase activity was carried out.

Experimental method: Tyrosinase is an enzyme involved in determining the initial rate of the most important step in the melanin biosynthetic pathway in the human body. Many whitening ingredients inhibit the enzyme. Thus, the ability to inhibit mushroom-derived tyrosinase can be evaluated to evaluate whitening efficacy.

The enzyme was assayed for its absorbance by reacting with [enzyme solution + substrate solution + sample (6.25, 12.5, 25 ug / mL)] for a certain period of time and measuring the absorbance. The concentration of the sample was not toxic to the B16- Respectively.

Experimental Results: The results of measuring tyrosinase inhibitory ability against pure white fruit oil and seed oil obtained according to Example 1 and Example 2 are shown in Table 1 and Table 1 below.

As a result, the inhibitory ability of Tyrosinase was relatively dependent on the concentration of arbutin, which is a positive control, and the effect of seed oil was similar to that of pure white fruit oil.

The positive control group, arbutin, was able to inhibit 16.6% tyrosinase activity at 25 ug / mL, whereas 25.0% and 22.1% inhibited Tyrosinase activity at 25 ug / mL concentration of pure fruit oil and seed oil Respectively.

Table 2 below shows the tyrosinase activity inhibition ability of pure hulled wood oil and seed oil obtained according to Examples 1 and 2 above.

division Tyrosinase activity
Inhibition (%) Remarks Positive control
[Arbutin] 6.25 / / mL 15.1 ± 2.6 12.50 占 퐂 / mL 15.9 ± 1.9 25.00 / / mL 16.6 ± 2.5
Example 1 6.25 / / mL 20.1 ± 0.3 12.50 占 퐂 / mL 20.6 ± 2.1 25.00 / / mL 25.0 ± 0.6
Example 2 6.25 / / mL 19.7 ± 0.3 12.50 占 퐂 / mL 20.5 ± 1.2 25.00 / / mL 22.1 ± 2.1

EXPERIMENTAL EXAMPLE 3 : Whitening improvement effect of pure fruit or seed oil of a crow tree (2): Effect of preventing melanin synthesis in cells

In order to confirm the whitening improvement effect of pure white fruit oil and seed oil obtained according to Examples 1 and 2, an experiment for evaluating the inhibitory effect on intracellular melanin synthesis was conducted.

Experimental Method: 3 days after culturing Murine melanoma of B16-F10 cell line under 1 × 10 after frequency division by ⁶ cell / well, were prepared for the measurement concentration of 6.25, 12.5, 25 ug / mL , 5% CO 2, 37 ℃ in 6well plate , Melanin was recovered from the cultured cells and the absorbance was measured.

Experimental Results: The results of measuring intracellular melanin synthesis inhibitory effects on pure white fruit oil and seed oil obtained according to Example 1 and Example 2 are shown in FIG. 2 and Table 3 below.

As a result, the ability to inhibit melanin synthesis in cells was inhibited in a concentration - dependent manner, and the effect of seed oil was similar to that of pure white fruit oil.

The intracellular melanin content of the seedless pure fruit oil of crows showed 82.9%, 60.6% and 41.8% at 6.25 ug / mL, 12.50 ug / mL and 25.00 ug / mL, respectively. The content of melanin was 75.5%, 66.3% and 49.2% at the concentration of 6.25 ug / mL, 12.50 ug / mL and 25.00 ug / mL, respectively.

Table 3 below shows the melanin contents (%) in the intracellular melanin synthesis inhibitory effect of seedless pure oil and seed oil obtained according to Examples 1 and 2 above.

division Melanin contents (%) Remarks
Negative control 6.25 / / mL 100 ± 2.6 12.50 占 퐂 / mL 100 ± 1.9 25.00 / / mL 100 ± 2.7 Positive control
[Arbutin] 25.00 / / mL 97.1 ± 2.6 50.00 / / mL 90.5 ± 2.5 100.00 / / mL 77.2 ± 2.6
Example 1 6.25 / / mL 82.9 ± 0.9 12.50 占 퐂 / mL 60.6 ± 0.6 25.00 / / mL 41.8 ± 0.3
Example 2 6.25 / / mL 75.5 ± 0.8 12.50 占 퐂 / mL 66.3 ± 0.6 25.00 / / mL 49.2 ± 0.3

The pure white fruit oil and seed oil of the present invention have excellent whitening improving effect. Therefore, according to the present invention, pure white fruit oil and seed oil of crows can be usefully used for products having whitening improving effect.

Claims (6)

The n-hexane, which is a solvent, is added to the crow's nuts at a ratio of 9-11 times the weight, and the mixture is stirred at room temperature for 24 hours to remove the n-hexane layer. To inhibit the production of melanin. The method according to claim 1,
Wherein the oil is obtained from any one of pure fruit or seed of a crow tree. The method according to claim 1,
The oil is seed oil obtained as a fruit oil obtained as a pure fruit of a crow tree or as a seed of a crow tree,
Wherein the pure fruit oil and the seed oil are used alone or in combination. N-hexane, which is a solvent, is added to the crow's nuts at a ratio of 9-11 times by weight,
The mixture was stirred at room temperature for 24 hours,
A method for preparing a crow's side tree oil for whitening improvement which inhibits melanin production obtained by removing n-hexane layer. 5. The method of claim 4,
Wherein the corn tree fruit is separated into pure fruit and seed, and then oil is separately prepared from the separated pure fruit and seed, respectively. delete

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