KR20160098571A - COMPOSITION COMPRISING EXTRACT OF Carex scabrifolia Steud HAVING ANTI-INFLAMMATORY AND ANTI-OXIDANT ACTIVITY - Google Patents
COMPOSITION COMPRISING EXTRACT OF Carex scabrifolia Steud HAVING ANTI-INFLAMMATORY AND ANTI-OXIDANT ACTIVITY Download PDFInfo
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- KR20160098571A KR20160098571A KR1020150019392A KR20150019392A KR20160098571A KR 20160098571 A KR20160098571 A KR 20160098571A KR 1020150019392 A KR1020150019392 A KR 1020150019392A KR 20150019392 A KR20150019392 A KR 20150019392A KR 20160098571 A KR20160098571 A KR 20160098571A
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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Abstract
Description
The present invention relates to an anti-inflammatory, antioxidant active pharmaceutical composition and a food composition containing a Carex scabrifolia Steud extract.
Inflammation is one of the defenses of the body against physical stimuli, chemical stimuli, bacterial infections, etc. It is one of the mechanisms to remove external substances and regenerate damaged areas. Once an inflammation-related stimulus is applied, vasoactive substances such as histamine, serotonine, bradykinin, prostaglandin, hydroxyeicosatetraenoic acid (HETE), leukotriene And the vascular permeability is increased to cause inflammation.
Monocytes are transformed into macrophages that are activated by bacterial infection and act as phagocytic cells. Macrophages induce inflammation by secretion of NO, prostaglandin E2, and proinflammatory cytokines, and regulatory cells important in both inflammation and immune response In order for macrophages to function in this way, they must undergo an activation process.
LPS (lipopolysaccharide), one of the cell wall components of Gram-negative bacteria and well known as endotoxin, is the most well-known external factor involved in macrophage activation. Particularly, in monocytes and macrophages such as RAW 264.7 cells, proinflammatory cytokines such as TNF-α, IL-6 (interleukin-6) and IL-1β (interleukin- -inflammatory cytokine secreted by LPS (lipopolysaccharide) in the inflamed area.
When LPS stimulates macrophages, nitric oxide (NO) is produced in the process of converting L-arginine into L-citrulline by an enzyme called iNOS (inducible nitric oxide synthase) Phagocytes produce NO.
In mammals, NO is synthesized by three kinds of NO synthase (NOS): nNOS (neuronal NOS), eNOS (endothelial NOS) and iNOS (inducible NOS). Among these, NO produced by nNOS and eNOS is produced for normal biological function, and the concentration in tissues is kept low to a certain level. However, the amount of NO produced by iNOS is excessively high, and it is harmful to living organisms such as pathological vasodilation, cytotoxicity and tissue damage. In addition, prostaglandin E2 (PGE2), an inflammatory factor, stimulates phospholipid, which is a constituent of cell membrane, stimulated by LPS, and arachidonic acid liberated by an enzyme called phospholipase A2 causes catalytic action of an enzyme called COXs (cyclooxygenase) And is induced to induce an inflammatory reaction.
COX is classified as COX-1 and COX-2. COX-1 acts on normal biological functions such as platelet formation, gastric wall protection and maintenance of renal function in the body. COX-2 synthesizes PHE2 as an inflammatory mediator. PHE2 is known to be deeply involved in the development of cancer, such as promoting inflammation (pain, fever, etc.), immune response, and angiogenesis. Inhibition of iNOS is mostly due to its mechanism of action being linked to inhibitors of COXs. Therefore, iNOS inhibition materials that are relatively easy to search are discovered and developed as anti-inflammatory materials.
On the other hand, non-steroidal anti-inflammatory drugs (NSAIDs), including selective COX-2 inhibitors, have anti-inflammatory, antipyretic and analgesic effects. However, the long-term use of these drugs can sometimes lead to serious side effects. For example, secondary anemia due to gastrointestinal peptic ulcer bleeding, platelet function suppression, inhibition of induction of labor, side effects to the kidney, liver damage, hypersensitivity.
In order to overcome the side effects of these drugs, natural products such as plants are more preferable as a safer material. In this regard, researches are being actively carried out to find and use the anti-inflammatory materials in natural products.
The antioxidant function inhibits or slows down the oxidation reaction occurring in the body. Oxidation reaction in the body induces aging and cell transformation to generate cancer cells. Therefore, the antioxidant activity is a preventive measure to suppress the negative reaction in the body, and the public is also interested.
On the other hand, in the body, mitochondria, which are main functions of ATP production, generate free radicals, which are byproducts of respiration, in addition to cell respiration, and they are unstable, And react indiscriminately. Therefore, if we can prevent the generation of these free radicals, we can prevent the oxidation of the body, and indirectly, we can prevent aging and cancer.
These antioxidant effects are difficult to experiment with free radicals occurring in the human body and use DPPH and ABTS radicals as biomarkers. The DPPH free radical scavenging activity assay is one of the methods for measuring the antioxidant activity of a sample. The DPPH free radical scavenging activity assay is a method for measuring the antioxidative activity of DPPH (1,1-diphenyl-2-picyl-hydrazyl) This method of decolorizing antioxidant activity is a widely used method because it can measure antioxidative activity in a relatively short time and has a high correlation with the antioxidant activity of plants.
Carex scabrifolia Steud is a perennial plant that grows in a salt marsh in the coastal and estuarine areas as a perennial plant that grows in a marsh by the sea. ( Careful distribution of saliva in the coastal area of Gyeonggi Bay, Shim Hyunbo et al. 20, No. 1, 2002 pp.25-34). It is characterized by small overall as compared to the big date of the first day. It is native to all parts of Korea and is also distributed in Taiwan, Russia, Japan, and China. No studies have been reported on the effect of improving the inflammation of this plant.
It is an object of the present invention to provide a composition containing a plant extract having excellent anti-inflammatory activity and antioxidative activity, wherein the component extracted from Carex scabrifolia steud has an inhibitory activity on inflammatory factor expression and a free radical scavenging activity And to provide a pharmaceutical composition and a food composition containing the same.
In order to achieve the above object, the anti-inflammatory composition according to an embodiment of the present invention contains a Carex scabrifolia Steud extract.
The sunflower seed extract may be one selected from the group consisting of alcohols having 1 to 5 carbon atoms, hexane, chloroform, alcohol, water, and mixtures thereof, and may be extracted by cold precipitation.
The sunflower seed extract is prepared by adding a solvent having a dry weight of 3 to 20 times by weight to a dry powder of sunflower seeds and extracting it at an extraction temperature of 20 to 120 ° C for 30 minutes to 10 days. And may be a powder from which the solvent has been removed from the extract.
The antiinflammatory composition is useful for the prevention or treatment of inflammatory diseases by containing a sunflower extract having anti-inflammatory activity which is confirmed to inhibit the production of NO induced by LPS in RAW 264.7 cells.
The antioxidant composition according to another embodiment of the present invention contains a Carex scabrifolia Steud extract.
The sunflower seed extract may be one selected from the group consisting of alcohols having 1 to 5 carbon atoms, hexane, chloroform, alcohol, water, and mixtures thereof, and may be extracted by cold precipitation.
The sunflower seed extract is prepared by adding a solvent having a weight of 3 to 20 times its dry weight to a dry powder of sunflower seeds and extracting it at an extraction temperature of 20 to 120 ° C for 30 minutes to 10 days. An extract or a powder in which the solvent is removed from the extract.
The antioxidant composition is useful as a pharmaceutical or food composition having an antioxidative activity by containing a sunflower extract having antioxidative activity, which is confirmed to erase DPPH free radicals.
A food composition according to another embodiment of the present invention contains a Carex scabrifolia Steud extract. The food composition has anti-inflammatory activity and antioxidative activity and can be used as a food composition in the form of a health functional food, a nutritional supplement, a health food, or a food additive.
Hereinafter, the present invention will be described in more detail.
The anti-inflammatory pharmaceutical composition according to one embodiment of the present invention contains the Carex scabrifolia Steud extract.
The sunflower seed extract can be obtained by extracting sunflower seeds, drying and crushing the powder and pulverizing it with a solvent. The dried and crushed sunflower seeds may be leaves, stems, roots, flowers, fruits, mixes or outposts of the first, second, and third sun, and the drying may be carried out in a known manner to the extent that useful components of the first- And can proceed, for example, by a method of natural drying in the shade. In addition, it is preferable that the crushing is performed after crushing to such an extent that useful components of the first day of the first day can be sufficiently extracted in the subsequent extraction process, and pulverized. The drying and crushing steps may be carried out in reverse order or repeatedly as required.
The extraction may be carried out by, for example, hot water extraction, cold extraction, warming extraction, reflux cooling extraction, ultrasonic extraction, or the like, preferably by a cold extraction method.
The extraction is carried out using a solvent, which may be an alcohol of 1 to 5 carbon atoms, hexane, chloroform, alcohol, water and mixtures thereof, preferably water, methanol, ethanol, .
The extraction is carried out at a temperature of 20 to 100 ° C, preferably 20 to 70 ° C, for 30 to 10 days, preferably 3 to 20 times, preferably 3 to 10 times, , Or an extraction time of 1 hour to 2 days. The method of the present invention can be applied to the production of the above pharmaceutical composition, 5 times, preferably 2 times to 3 times, to obtain a liquid-phase extract.
The chrysanthemum morifolium extract may be separated from the supernatant by centrifugation or filtration, and then concentrated or dried. For example, the above-mentioned liquid phase sunflower extract may be concentrated in a vacuum rotary condenser at 20 to 100 ° C, preferably 40 to 70 ° C, and the liquid phase of the sunflower seed extract may be dried to obtain a powdered sunflower seed Extracts may also be obtained. The thus-concentrated or powdered sunflower seed extract can be used as needed in water, an alcohol or a mixed solvent thereof. In addition, the liquid phase of the sunflower seed extract, its concentrate or powder thereof may be an extract obtained by fractionation using nucleic acid or ethyl acetate or fractional stroke of the fraction.
The chrysanthemum morifolium extract is useful as a pharmaceutical composition for antiinflammatory activity by inhibiting the expression of iNOS which is an inflammation mediator and inhibiting the expression of NO produced by the enzyme and containing components useful for the prevention and treatment of inflammation related diseases And can be used as a pharmaceutical composition for the prevention or treatment of inflammatory diseases, and provides a pharmaceutical composition for preventing or treating inflammation caused by infection from external bacteria.
The anti-inflammatory composition may further comprise additional components and may further comprise at least one of, for example, a carrier, excipient, and diluent, for example, lactose, dextrose, sucrose, sorbitol, But are not limited to, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate Tartrate, magnesium stearate, and mineral oil.
The antiinflammatory composition may have various formulations and may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, external preparations such as syrups and aerosols, have. In the case of formulation, it can be prepared using diluents or excipients such as fillers, extenders, binders, humectants, disintegrants, surfactants and the like which are usually used, and the preparations for non-oral administration include sterilized aqueous solutions, Emulsions, freeze-dried preparations, suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include withexol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.
The preferred dosage of the anti-inflammatory composition may be appropriately selected depending on the condition and body weight of the patient, the degree of disease, the form of the drug, the route of administration and the period of time. However, for the desired anti-inflammatory effect, the extract or the compound of the present invention can be administered in an amount of 0.01 to 500 mg / kg per day, preferably 0.1 to 200 mg / kg per day, divided once to several times per day. The dose of the compound may also be increased or decreased depending on the route of administration, degree of disease, sex, weight, age, and the like.
The antioxidant composition according to another embodiment of the present invention contains a Carex scabrifolia Steud extract. Among the descriptions of the antioxidant composition, descriptions overlapping with the anti-inflammatory composition are omitted. The antioxidant composition may be used as an active ingredient or adjuvant of a pharmaceutical composition, and may be used as an active ingredient or an additional ingredient in an antioxidant.
The food composition according to another embodiment of the present invention contains an extract of Carex scabrifolia Steud and has an anti-inflammatory and antioxidative function and can be used as a health food, a nutritional supplement, a health food, Can be utilized as a composition.
Food compositions of this type can be prepared in a variety of forms according to conventional methods. For example, the health food may be prepared by liquefying, granulating, encapsulating, and pulverizing the chrysanthemum morifolium extract itself in the form of tea, juice, and drink so that it can be consumed. In addition, the sunscreen extract of the present invention may be mixed with a known active ingredient known to have anti-inflammatory effects to prepare a composition. Functional foods also include, but are not limited to, beverages (including alcoholic beverages), fruits and processed foods such as canned fruits, jars, jam, maare marlade, fish, meat and processed foods (Eg butter, cheeses, etc.), edible vegetable oils (eg, sourdoughs, etc.), breads and noodles (eg udon, buckwheat noodles, ramen noodles, spaghetti, macaroni, , Margarine, vegetable protein, retort food, frozen food, various kinds of seasoning (e.g., soybean paste, soy sauce, sauce, etc.). The preferred content of the sunflower seed extract of the food composition is not particularly limited, but is preferably 0.01 to 50% by weight in the finally prepared food.
The anti-inflammatory composition, antioxidative composition and food composition of the present invention contain an extract of Carex scabrifolia steud for inhibiting the expression of an inflammatory factor and antioxidant activity, and can be usefully used as a pharmaceutical composition or a functional food composition.
FIG. 1 shows the results of evaluating the amount of NO produced from the samples treated with the chrysanthemum extract prepared in Example 1 of the present invention.
FIG. 2 shows the results of evaluating the iNOS activity produced from the samples treated with the sunflower seed extract prepared in Example 1 of the present invention.
FIG. 3 shows the results of evaluating the antioxidative activity of the sunflower seed extract prepared in Example 1 of the present invention using the DPPH free radical-scavenging activity assay.
Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.
Example 1: Preparation of sunflower seed extract
The outcrops of the domestic naturally occurring sunflower seeds ( Carex scabrifolia Steud ) were collected, dried for 14 days in the shade, and then crushed to a size of 20 mesh or less.
1 liter of ethanol was added to 100 g of the dried powder of the sunflower latex, and the mixture was cooled at 25 DEG C for 7 days. The mixture was extracted three times repeatedly and filtered through a filter paper (Wattsman, USA) to obtain a filtered extract. Ethanol was removed from the filtrate extract at 40 ° C using a vacuum rotary condenser to obtain 12 to 20 g of a chrysanthemum morifolium extract residue. 10 mg of the sample was used as a sample for the detection of the anti-inflammatory activity.
Example 2: Anti-inflammatory activity of sunflower seed extract
The antioxidant activity of the extracts of chrysanthemum morifolium was confirmed by measuring the amount of NO and by using iNOS (inducible nitric oxide synthase) assay.
RAW 264.7 cells were inoculated into a 24-well plate at a density of 10 x 10 cells / well using DMEM medium and cultured for 12 hours. The RAW 264.7 cells were inoculated with 20, 4, 0.8, or 0.16 ug / ml and LPS (1 μg / ml) at the same time, and cultured for 24 hours. From the cultured samples, the amount of NO 2 - present in the cell culture was measured using a Griess reagent, and the amount of NO produced and iNOS activity were measured. Specifically, 500 μl of the cell culture supernatant of the above cultured samples and 50 μl of a grease reagent (2.5% sulfanilamide, 0.25% naphthylethylenediamine dissolved in 10% phosphoric acid) were mixed and reacted on a 24-well plate for 10 minutes, The absorbance was measured at 540 nm using a reader. The standard concentration curve was obtained by serial dilution of DMSO. The amount of NO produced and the activity of iNOS were evaluated. The results are shown in FIGS. 1 and 2 and Table 1. In addition, the antiinflammatory activity was compared between the negative control and the positive control using aspirin in Comparative Examples 1 and 2. The aspirin used inhibited iNOS activity by 50% at a concentration of about 3.2 μg / mL , And 100 μg / mL, respectively.
(Aspirin)
(Negative control)
Referring to FIG. 1, it was confirmed that the chrysanthemum morifolium extract effectively reduced the production of NO in RAW 264.7 c cells induced by LPS, and it was also confirmed that the degree of reduction was concentration-dependent. NO is synthesized from L-arginine by nitric oxide synthase (NOS). Under pathologic conditions, increased NO promotes the development of inflammation, which is synthesized by iNOS, a synergistic agent with other inflammatory mediators. In addition, iNOS is strongly stimulated by exposure to inflammatory cytokines and bacterial endotoxins. Therefore, a substance capable of reducing NO production by iNOS can be utilized as an anti-inflammatory agent.
In addition, referring to FIG. 2 and Table 1, it was also confirmed that the chrysanthemum morifolium extract showed an inhibitory effect on iNOS in RAW 264.7 cells induced by LPS, and that it was concentration-dependent in relation to the chrysanthemum morifolium extract. NO produced by activated iNOS in inflammatory conditions has deleterious effects on the organism such as pathological vasodilation, cytotoxicity, and tissue damage. RAW 264.7 showed a strong inhibitory effect on the production of NO and proinflammatory cytokines by treatment with LPS for the treatment of sunflower seed extracts. The chrysanthemum morifolium extract showed a concentration-dependent decrease in LPS-induced NOS production and that the chrysanthemum morifolium extract had a strong inhibitory effect on iNOS protein expression in RAW 264.7 cells induced by LPS .
Example 3 Evaluation of Antioxidative Activity of Sunflower Seedlings Extract
The antioxidant activity of the chrysanthemum morifolium extract prepared in Example 1 was measured using a DPPH free radical scavenging activity test. Twenty microliters of TBHQ (positive control), methanol (negative control), and methanol solution (2000, 1000, 500, 250, and 65.5 μg / ml) were added to each well of a 96-well microplate. DPPH (Sigma chemical Co. USA) methanol solution was added. The plate was wrapped with a foil to block the light, reacted at room temperature for 20 minutes, and the absorbance at 517 nm was measured. The results are shown in FIG.
Referring to FIG. 3, it was confirmed that the sunscreen extract of DPPH scavenged DPPH free radicals in a concentration-dependent manner, and it was confirmed that DPPH free radicals had excellent antioxidative activities like quinones having antioxidative functions.
While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, Of the right.
Claims (5)
Wherein the chrysanthemum morifolium extract is extracted by cold precipitation with any one selected from the group consisting of alcohols having 1 to 5 carbon atoms, hexane, chloroform, alcohol, water, and mixtures thereof.
The sunflower seed extract is prepared by adding a solvent having a dry weight of 3 to 20 times by weight to a dry powder of sunflower seeds and extracting it at an extraction temperature of 20 to 120 ° C for 30 minutes to 10 days. Wherein the solvent is removed from the extract.
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| KR1020150019392A KR20160098571A (en) | 2015-02-09 | 2015-02-09 | COMPOSITION COMPRISING EXTRACT OF Carex scabrifolia Steud HAVING ANTI-INFLAMMATORY AND ANTI-OXIDANT ACTIVITY |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102086854B1 (en) * | 2018-10-05 | 2020-03-11 | 대한민국 | Anti-oxidant or anti-inflammatory cosmetic compositions containing the plants extract of Cyperaceae |
| KR20210035594A (en) * | 2019-09-24 | 2021-04-01 | 한국해양대학교 산학협력단 | Compositions comprising Carex pumila extract or its organic solvent fraction for inhibiting activities of matrix metalloproteinases and method for producing the same |
| CN114146133A (en) * | 2021-12-21 | 2022-03-08 | 吉林省蒲川生物医药有限公司 | Application of carex breviculmis in preparation of anti-aging product |
-
2015
- 2015-02-09 KR KR1020150019392A patent/KR20160098571A/en not_active Application Discontinuation
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102086854B1 (en) * | 2018-10-05 | 2020-03-11 | 대한민국 | Anti-oxidant or anti-inflammatory cosmetic compositions containing the plants extract of Cyperaceae |
| KR20210035594A (en) * | 2019-09-24 | 2021-04-01 | 한국해양대학교 산학협력단 | Compositions comprising Carex pumila extract or its organic solvent fraction for inhibiting activities of matrix metalloproteinases and method for producing the same |
| CN114146133A (en) * | 2021-12-21 | 2022-03-08 | 吉林省蒲川生物医药有限公司 | Application of carex breviculmis in preparation of anti-aging product |
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