KR101554696B1 - Mobile bio-scaffold controlled by magnetic field and manufacturing method thereof - Google Patents

Abstract

Provided is a mobile bio-scaffold controlled by a magnetic field. The mobile bio-scaffold comprises: a spiral body including a first body formed in a spiral shape for a first surface and a second surface to face each other, and including multiple first holes for culturing cells in the first body; and a magnetic layer formed on the outer surface of the spiral body, wherein the spiral body is moved by being rotated in one direction by an interaction between a rotatory magnetic field applied from the outside and a magnetic layer.

Description

TECHNICAL FIELD [0001] The present invention relates to a mobile bio-carrier capable of controlling a magnetic field and a manufacturing method thereof,

The present invention relates to a moving body biomaterial that can control a magnetic field and a manufacturing method thereof. More particularly, the present invention relates to a moving body biomaterial that can control the movement of a living body carrier by a magnetic field applied from the outside, And a manufacturing method thereof.

Recently, the field of tissue engineering for the treatment and regeneration of tissues has been developed in the field of biotechnology.

Tissue engineering is to maintain and improve the function of our body by understanding the correlation between the structure and function of living tissue and by making a substitution of living tissue and implanting it into the body again by integrating and applying basic concepts and technologies of life sciences and engineering. Or restoring it.

However, despite the rapid growth of medical engineering technology and its high level, damage to human organs and tissues frequently occurs, and the organ transplant surgery to treat it involves technical difficulties, high cost, lack of donors and immunity Side effects due to the use of inhibitors, and the like.

Therefore, there is a great need for the development of artificial organs or tissue regeneration using tissue engineering as a new approach to organ transplantation.

The basic principle of the tissue engineering is to collect the necessary tissues from the body of the patient, to separate the cells from the tissue, and then to separate the cells into a support to prepare a cell-support complex, .

Such a cell-support must satisfy various conditions as well as safety in vivo. First, it should be made of a material that helps the cell adhesion, proliferation, and differentiation activity. The support should be made of a porous structure capable of promoting cell proliferation and tissue regeneration throughout the support. This should be good.

In addition, the biocompatible body must have biocompatibility, and it is required to use a biodegradable material depending on the applied position, so as to provide a large surface area so that the cells and tissues to be implanted can be easily integrated.

The currently used bioconjugates are mainly used for the regeneration of bones, skin, organs, and the shape and pore size of the cells and tissues of the transplants are determined. The formation of new structures on the structure is very important because they are greatly influenced by the porosity and size of the structure and the three-dimensional porous interconnection structure. A suitable porous structure is needed to carry a sufficient number of cells, and interconnected porous structures are necessary for nutrient diffusion.

Recently, research and development for producing a biosuppressor that can be used more effectively and stably for the treatment and regeneration of such tissues has been continued.

Accordingly, in Korean Patent Laid-Open No. 10-2013-0120572 (published on November 1, 2013 (May 31, 2013)), a porous three-dimensional structure containing cells and a method for producing the same are disclosed.

However, conventionally, there has been a difficulty in directly inserting such a support into a living body and locating it at a desired position with the help of a surgical method and a machine in order to place it in a living body. In addition, such a method has a problem in that there is a risk of infection and trauma in the insertion process, and there is a limit in application to a dangerous part when exposed to external parts such as a local site or a blood vessel or brain tissue which is difficult to access.

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems and it is an object of the present invention to provide a mobile body-transportable body having excellent biocompatibility and being capable of being precisely and quickly moved to a target point by a magnetic field applied from the outside, There is a purpose.

It is another object of the present invention to provide a movable body member capable of controlling a magnetic field capable of improving propelling efficiency when the body support is spirally rotated by an external magnetic field.

In order to accomplish the above object, the present invention provides a method of manufacturing a medical device, which comprises a first body formed in a spiral shape so as to face a first surface and a second surface, A helical body having a plurality of first pores; And a magnetic layer formed on an outer surface of the helical body, wherein the helical body rotates and moves in one direction by an interaction between a rotating magnetic field applied from the outside and a magnetic layer, Lt; / RTI >

Preferably, the helical body may include a second body connecting between the first surface and the second surface facing each other.

Preferably, the second body has a plurality of second pores on an outer surface thereof.

Preferably, the helical body may include a third body having an inner space at one end of the first body.

Preferably, the third body has a plurality of third pores on an outer surface thereof, so that the cells can be cultured in the inner space or the drug can be stored.

Preferably, the first body may be formed so that the first face and the second face that face each other have the same width or are formed to have different widths.

Preferably, the helical body may be formed of a resin material, a biodegradable material that is naturally decomposed in the human body, and a biocompatible magnetic material or a combination thereof.

Preferably, the magnetic layer may be wholly or partially covered with the helical body.

Preferably, the helical body may have a protective layer made of a biocompatible material.

According to another aspect of the present invention, there is provided a method of manufacturing a microporous membrane, comprising: forming a helical body having a first body formed in a spiral shape so that a first surface and a second surface face each other; And forming a magnetic layer on the outer surface of the helical body so that the helical body interacts with a rotating magnetic field externally applied to the helical body.

Preferably, after the step of forming the helical body, a step of forming a second body having one end on the first surface and the other end on the second surface, the second body connecting the first surface and the second surface facing each other .

The method may further include forming a third body having an internal space at one end of the first body after the step of forming the body.

Preferably, after the step of forming the magnetic layer, a step of forming a protective layer made of a biocompatible material to cover the magnetic layer may be further included.

More preferably, after the magnetic layer forming step, the step of culturing the cells in the helical body or storing the drug may be further included.

According to the present invention as described above, the following effects can be obtained.

(1) Since biocompatibility is excellent, it is possible to prevent the occurrence of side effects in the process of inserting into the human body.

(2) Since the movement of the living body support can be controlled by the rotating magnetic field applied from the outside, the cells can be directly transferred to the local site, blood vessels and brain tissue without the aid of the surgical method and the machine.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is an overall perspective view of a movable body-transportable living body carrier according to a first embodiment of the present invention; FIG.
FIG. 2 is a modification of the movable body-movable body carrier according to the first embodiment of the present invention.
FIG. 3 is a use state diagram showing a cell culture state in a mobile body-transportable living body carrier according to the first embodiment of the present invention.
4 is an overall perspective view of a movable body-transportable living body carrier according to a second embodiment of the present invention.
FIG. 5 is a use state diagram showing a cell culture state in a movable body-transportable body carrier according to a second embodiment of the present invention.
6 is an overall perspective view of a movable body-transportable living body carrier according to a third embodiment of the present invention.
FIG. 7 is a use state diagram showing a cell culture state in a mobile body-transportable living body carrier according to a third embodiment of the present invention.
8 is an overall perspective view of a movable body-transportable living body carrier according to a fourth embodiment of the present invention.
9 is a cross-sectional enlarged view of a movable body-transportable body member according to an embodiment of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the following description, a detailed description of known functions and configurations incorporated herein will be omitted when it may obscure the subject matter of the present invention.

The same reference numerals are used for portions having similar functions and functions throughout the drawings.

In addition, in the entire specification, when a part is referred to as being 'connected' to another part, it may be referred to as 'indirectly connected' not only with 'directly connected' . Also, to include an element does not exclude other elements unless specifically stated otherwise, but may also include other elements.

A movable body-transportable body-transportable body and a method of manufacturing the same according to a preferred embodiment of the present invention can control the movement of a living body body by a rotating magnetic field applied from the outside, thereby enabling cells to be delivered to local parts such as internal tissues or microvessels There are technical features.

The biological support capable of controlling the magnetic field according to the preferred embodiment of the present invention includes a helical body 10, 10 ', 10' ', 10' 'and a magnetic layer 200.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is an overall perspective view of a movable body-transportable living body carrier according to a first embodiment of the present invention; FIG.

1, the helical body 10 includes a first body 100 having a first surface 110 and a second surface 120, and the cell 20 is transported to a target point The helical body 10 is formed in a spiral shape in which the first surface 110 and the second surface 120 face each other by twisting the first body 100 in one direction about a virtual axis to increase the transport efficiency of the helical body 10 do.

In this case, the first body 100 has the first face 110 and the second face 120 having the same width (W) repeatedly formed facing each other. However, the first body 100 is not limited thereto, The first surface W1 and the second surface W2 having different widths are formed so as to face each other and one of the surfaces facing each other with increasing width toward the one side or W1 < And the like.

The first body 100 may be formed such that the first face 110 and the second face 120 facing each other are repeatedly formed to have the same pitch L1 = As shown in FIG.

FIG. 3 is a use state diagram showing a cell culture state in a mobile body-transportable living body carrier according to the first embodiment of the present invention.

Referring to FIG. 3, a plurality of first pores 130 are provided on the outer surface of the first body 100 to increase the surface area where the cells can be cultured.

Thus, a larger amount of cells can be cultured in the helical body 10.

Here, the first pores 130 are shown in a trapezoidal shape, but the present invention is not limited thereto. The first pores 130 may be formed in various shapes such as a circular shape, a triangular shape, a polygonal shape, and the like.

4 is an overall perspective view of a movable body-transportable living body carrier according to a second embodiment of the present invention.

Referring to FIG. 4, the helical body 10 'is provided with a first body 100 and a second body 200.

One end of the second body 200 is provided on the first surface 110 and the other end of the second body 200 is provided on the first surface 110. The first body 110 has a first surface 110 and a second surface 120 facing each other, And is provided on the second surface 120 to connect between the first surface 110 and the second surface 120 of the first body 100.

Accordingly, the helical body 10 'is provided with an inner space by the second body 200, and the cells 20 are cultured in the first pores 130 and the inner space to transfer the cells to the first target site .

FIG. 5 is a use state diagram showing a cell culture state in a movable body-transportable body carrier according to a second embodiment of the present invention.

Referring to FIG. 5, on the outer surface of the second body 200, a plurality of second pores 210 through which the cells 20 can be cultured are formed. The second pores 210 may be formed in various shapes such as a circular shape, a triangle shape, and a polygonal shape, and may be formed in the same size or different sizes.

Accordingly, cells are cultured in the first pores 130 and the second pores 210, so that a larger amount of the cells 20 can be cultured in the honeycomb 10 '.

6 is an overall perspective view of a movable body-transportable living body carrier according to a third embodiment of the present invention.

Referring to FIG. 6, the helical body 10 '' includes a first body 100 and a third body 300.

The first body 100 includes a third body 300 having the shape of a cylindrical body having an inner space at one end of the first body 110 in the shape of a helical body in which the first surface 110 and the second surface 120 face each other, . Accordingly, the third body 300 rotates together with the first body 100 during operation of the helical body 10 "to transfer the cells 20 to the first target point.

Here, although the third body 300 is shown as a cylindrical shape, the third body 300 is not limited thereto and may be provided in various shapes such as a rectangular tube, a cone, and the like.

Also, although the third body 300 is shown at one end of the first body 100, the third body 300 is not limited thereto and may be provided at both ends of the first body 100.

FIG. 7 is a use state diagram showing a cell culture state in a mobile body-transportable living body carrier according to a third embodiment of the present invention.

Referring to FIG. 7, a plurality of third pores 310 through which the cells 20 are cultured are formed on the outer surface of the third body 300. The third pores 310 may be formed in various shapes such as a circular shape, a triangle shape, a polygonal shape, and the like. The third pores 310 may have the same size or different sizes.

Accordingly, the helical body 10 "can transfer a large amount of cells 20 to the target point initially set by culturing the cells in the first pores 130 and the third pores 310.

8 is an overall perspective view of a movable body-transportable living body carrier according to a fourth embodiment of the present invention.

Referring to FIG. 8, the helical body 10 '' includes a first body 100, a second body 200, and a third body 300.

The first body 100 has a spiral shape in which the first face 110 and the second face 120 face each other. The second body 200 has a first face 110 and a second face 110, And is provided on the second surface 120 to connect the first surface 110 and the second surface 120 of the first body 100 to each other.

The third body 300 is provided at one end of the first body 100 and is a cylindrical body having an inner space.

Here, although the third body 300 is shown as a cylindrical shape, the third body 300 is not limited thereto, and may be formed in various shapes such as a rectangular tube, a cone shape, and the like.

Accordingly, the third body 300 rotates with the first body 100 and transfers the cells 20 to the target point during the operation of the helical body 10 ". Here, 1 body 100, but it is not limited thereto and may be provided at both ends of the first body 100.

A plurality of first pores 130 through third pores 310 are formed on the outer surfaces of the first body 100, the second body 200 and the third body 300, . Here, the first to third pores 130 to 310 may be formed in various shapes such as a circular shape, a triangle shape, and a polygonal shape, and may be formed in the same size or different sizes.

The first to third pores 130 to 310 may include only the first pores 130 and the second pores 210 or may include only the first pores 130 and the third pores 310, As shown in FIG.

Accordingly, the helical body 10 '' 'can transfer cells to the original target site by culturing the cells 20 in the first pores 130, the second pores 210 and the third pores 310 .

9 is a cross-sectional enlarged view of a movable body-transportable body member according to an embodiment of the present invention.

Referring to FIG. 9, a magnetic layer 30 and a protective layer 40 may be formed on the outer surface of the helical body 10, 10 ', 10', 10 '' '.

The helical body 10, 10 ', 10' ', 10' '' is not particularly limited as long as it is a material and a form that can be used as a conventional living body carrier, and is made of a resin material such as a polymer, a ceramic, or a nanofiber, Biodegradable materials, biocompatible magnetic materials, and the like, which can be naturally degraded.

Preferably, the helical bodies 10, 10 ', 10' 'and 10' '' may be manufactured by a lithography method using a photocurable polymer so as to form micro-sized cells which are easily transported in vivo.

Herein, the photocurable polymer is a polymer that cures upon irradiation of light, and is not particularly limited as long as it can form a three-dimensional body carrier through lithography. More preferably SU-8 polymer, KMPR, IP-L or IP-G, or the like. Most preferably SU-8 polymer.

Since the helical body 10, 10 ', 10' ', 10' '' can control the movement of the living body carrier by the external magnetic field by forming the magnetic layer 30 on the outer surface, And the bioconjugate can be placed at the transplantation site.

The magnetic layer 30 is preferably made of a metal having a magnetic property of a certain intensity and a small amount of corrosive (reactivity), and more preferably a metal such as nickel (Ni), iron (Fe), cobalt (Co), or neodymium ), And the like, or may be in a mixed form, and most preferably, it may include nickel (Ni).

Accordingly, the helical bodies 10, 10 ', 10 "and 10'" move in one direction by the interaction between the rotating magnetic field applied from the outside and the magnetic layer 200, Can be transferred.

Here, the magnetic layer 30 is formed on the entire outer surface of the helical body 10, 10 ', 10', 10 '' ', but the present invention is not limited thereto. 10 ', 10 ", 10' '' selectively formed in the three bodies 300 or formed at the joint between the body and the body.

The biotransporter is implanted into a human body using a tool such as an endoscope not shown in the form of a cell-support complex by culturing the cells 20 to be implanted in the pores or the outer surface of the body, It is important.

Accordingly, the helical body 10, 10 ', 10' ', 10' '' is provided with a protective layer 40 covering the entire outer surface so as not to have a side effect in vivo. However, the present invention is not limited thereto, and the protective layer 40 may be formed to cover the entire outer surface of the magnetic layer 30.

Here, the protective layer 40 is preferably made of a material having excellent biocompatibility, and may be a single or a mixture of titanium (Ti), medical stainless steel, alumina (Al ₂ O ₃ ), gold (Au) And preferably titanium (Ti).

The movable body-carrier capable of controlling the magnetic field and the method of manufacturing the same are described as follows.

The magnetic field controllable biological support of the present invention comprises the steps of: forming a helical body; And forming a magnetic layer on the outer surface of the helical body.

The step of forming the helical body 10 is a main body in which the cells 20 are cultured and forms a helical body 10 capable of transporting the cells 20. The first surface 110 and the second surface The first body 100 having a strip shape is twisted in one direction around a virtual axis so that the helical body 10 having the first surface 110 and the second surface 120 face each other . At this time, the first body 100 forms a plurality of first pores 130 in order to increase the surface area where the cells are cultured.

Examples of the method for producing such a helical body include a method for producing a living body support, such as a particulate leaching method, an emulsion freeze-drying method, a high pressure gas expansion method and a phase separation method separation, Fused Deposition Modeling (FDM) or lithography.

Preferably, the bio-scaffold capable of controlling the magnetic field can be a three-dimensional bio-scaffold produced by a lithography method using a photocurable polymer.

The step of forming the magnetic layer may include forming a magnetic layer 30 on the outer surface of the helical body 10 so that the helical body 10 interacts with a rotating magnetic field applied from the outside, This is to control the movement.

The magnetic layer 30 may be formed by a conventional method such as electron beam evaporation, dipping, electrolytic plating, sputtering, or chemical vapor deposition Can be coated.

Accordingly, the magnetic field controllable body support according to the present invention can be applied to a dangerous area exposed to the outside such as a local site, blood vessels and brain tissue without using a surgical method or a machine as in the prior art, Lt; RTI ID = 0.0 &gt; 20 &lt; / RTI &gt;

The first surface 110 is provided at one end and the other end is provided at the second surface 120 to face the first surface 110 so as to widen the surface area of the helical body 10, And a second body (200) connecting the second surface (120). Accordingly, the helical body 10 'has an internal space of a predetermined size, thereby increasing the surface area at which the cells can be cultured. Here, a plurality of second pores 210 may be provided on the outer surface of the second body 200.

Preferably, a third body 300 having an inner space is provided at one end of the first body 100 to expand the cell culture space after the hull forming step. Here, the third body is shown as a cylindrical shape, but is not limited thereto, and may be provided in various forms such as a sphere, a cone, or the like, and may be provided at one end as well as at one end of the first body 100.

Preferably, after the magnetic layer forming step, a protective layer 40 made of a biocompatible metal may be coated on the outer circumferential surface of the magnetic layer 30 in order to improve in vivo stability and biocompatibility.

The biocompatible metal is not particularly limited as long as it is stable in vivo and has excellent biocompatibility. More preferably, the biocompatible metal is a metal such as titanium (Ti), medical stainless steel, alumina (Al ₂ O ₃ ) Or mixed, and most preferably titanium (Ti).

As with the coating of the magnetic material, the coating method is not particularly limited as long as it is a conventional coating method, but it can be more preferably coated by an electron beam deposition method, a dipping method, an electrolytic plating method, a sputtering method or a chemical vapor deposition method.

Preferably, after the magnetic layer forming step, the cells to be transported to the target site may be cultured or the drug may be stored in the inner space of the first pores 130 to the third pores 310 and the helical body.

Accordingly, the present invention provides a biomedical support capable of controlling the magnetic field and a method of manufacturing the biomedical support, which are excellent in biocompatibility. Therefore, it is possible to prevent the occurrence of side effects in the process of insertion into a human body, It is possible to directly transfer the cells to a local site, blood vessels and brain tissues without the help of a surgical method and a machine.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the inventions. It will be apparent to those of ordinary skill in the art.

10,10 ', 10 "10,"": Spiral 20: Cell
30: magnetic layer 40: protective layer
100: first body 110: first side
120: second side 130: first stage
200: second body 210: second porosity
300: Third Body 310: Third Porous

Claims (14)

And a first body formed in a spiral shape so that the first surface and the second surface face each other, wherein the first body has a plurality of first pores so as to be cultured or stored by attaching cells to the first body, ; And
And a magnetic layer formed on an outer surface of the helical body,
Wherein the helical body rotates and moves in one direction by an interaction between a rotating magnetic field applied from the outside and a magnetic layer. The method according to claim 1,
Wherein the helical body includes a second body connecting between the first surface and the second surface facing each other. 3. The method of claim 2,
Wherein the second body has a plurality of second pores on an outer surface thereof. 3. The method according to claim 1 or 2,
Wherein the helical body comprises a third body having an inner space at one end of the first body. 5. The method of claim 4,
Wherein the third body has a plurality of third pores on an outer surface thereof, wherein the cells are cultured in the inner space or the drug is stored. The method according to claim 1,
Wherein the first body is formed so that the first face and the second face that face each other have the same width or are repeatedly formed to have different widths. The method according to claim 1,
Wherein the helical body is formed of a resin material, a biodegradable material that is naturally decomposed in the human body, and a biocompatible magnetic material, or a combination thereof. 8. The method of claim 1 or 7,
Wherein the magnetic layer is wholly or partially covered with the helical body. 8. The method of claim 1 or 7,
Wherein the helical body comprises a protective layer made of a biocompatible material. Forming a helical body having a plurality of first pores so as to be cultured by attaching cells to the first body, the first body being formed in a spiral shape so that the first and second surfaces face each other; And
And forming a magnetic layer on an outer surface of the helical body so that the helical body interacts with a rotating magnetic field applied from the outside. 11. The method of claim 10,
After the helicoid formation step,
Further comprising the step of forming a second body, one end of which is provided on the first surface and the other end is provided on the second surface and which connects the first surface and the second surface facing each other, A method for manufacturing a mobile body carrier. The method according to claim 10 or 11,
After the helicoid formation step,
Further comprising forming a third body having an inner space at one end of the first body. &Lt; Desc / Clms Page number 19 &gt; The method according to claim 10 or 11,
After the magnetic layer forming step,
And forming a protective layer made of a biocompatible material so as to cover the magnetic layer. The method according to claim 10 or 11,
After the magnetic layer forming step,
Wherein the method further comprises the step of culturing the cells in the helical body or storing the drug.

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