KR101574585B1 - Mobile bio-scaffold controlled by magnetic field - Google Patents

Abstract

And provides a movable bio-scaffold with magnetic field control.
The present invention relates to a three-dimensional three-dimensional porous bio-scaffold comprising a photocurable polymer; And a magnetic layer coated on the surface of the living body support to a predetermined thickness so as to position the living body support inserted into the living body by a magnetic field provided from the outside to a desired position; .

Description

[0001] The present invention relates to a mobile bio-scaffold controlled by magnetic field,

Field of the Invention [0002] The present invention relates to a movable body support capable of controlling a magnetic field, and more particularly, to a movable body support capable of controlling a movement of a living body support by an external magnetic field to reach a desired position.

Recently, the field of tissue engineering for the treatment and regeneration of tissues has been developed in the field of biotechnology. Tissue engineering is to understand the relationship between structure and function of biomedical tissues by integrating basic concepts and technologies of bioscience and engineering, and to maintain, enhance, or improve the function of our body by implanting biomedical tissue substitute in the body again. It is an applied discipline aiming at restoration.

Despite the rapid growth of high-level medical engineering technology, damage to organs or tissues of the human body occurs frequently, and organ transplant surgery to treat it is technically difficult, costly, lack of donors and use of immunosuppressive drugs And the side effects caused by it.

As a new approach to organ transplantation, there is a great need for the development of artificial organs or tissue regeneration using tissue engineering. The basic principle of tissue engineering is to take the necessary tissues from the body of the patient, to separate the cells from the tissue, to cultivate the separated cells on a support to prepare a cell-support complex, and then transplant the cell-support complex into the body .

Such a cell-support must satisfy various conditions as well as safety in vivo. First, it should be made of a material that helps the cell adhesion, proliferation, and differentiation activity. The support should be made of a porous structure capable of promoting cell proliferation and tissue regeneration throughout the support. This should be good. The biocompatible body must have biocompatibility and have pores to provide a large surface area for easy integration of the cells and tissues to be transplanted. In some cases, the biodegradable material should be used depending on the applied position. Currently used biomedical scaffolds are mainly used for regeneration of bones, skin, organs, etc., and the shape and pore size of the cells and tissues of the biomedical scaffold are determined. The formation of new structures on the structure is a very important factor because it depends heavily on the porosity, size and three-dimensional multispace connection structure of the structure. A suitable porous structure is needed to carry a sufficient number of cells, and interconnected porous structures are necessary for nutrient diffusion.

 Research and development for producing a living body scaffold that can be more effectively and stably used for the treatment and regeneration of such tissues has been continuing in recent years.

However, conventionally, there has been a difficulty in directly inserting such a support into a living body and locating it at a desired position with the help of a surgical method and a machine in order to place it in a living body. In addition, such a method has a problem in that there is a risk of infection and trauma in the insertion process, and there is a limit in application to a dangerous part when exposed to external parts such as a local site or a blood vessel or brain tissue which is difficult to access.

Disclosure of the Invention The present invention has been conceived to solve the above-mentioned problems, and it is an object of the present invention to provide a mobile biomedical scaffold which can control a movement of a living body support by an external magnetic field, The purpose of this is to provide.

In order to solve the above-mentioned problems, the present invention provides a three-dimensional porous bio-scaffold comprising a photocurable polymer; And a magnetic layer coated on the surface of the living body support to a predetermined thickness so as to position the living body support inserted into the living body by a magnetic field provided from the outside to a desired position; . ≪ / RTI >

According to a preferred embodiment of the present invention, a biocompatible metal layer coated on the magnetic layer; . ≪ / RTI >

According to another preferred embodiment of the present invention, the cells may be cultured in the pores of the magnetic field controllable mobile body support.

According to another preferred embodiment of the present invention, the porous bio-support may have a three-dimensional structure including a photocurable polymer.

According to another preferred embodiment of the present invention, the porous bio-support may be cylindrical, hexahedral, elliptical, polyhedral or conical.

According to another preferred embodiment of the present invention, in the XYZ coordinate system, the porous biocompatible member may be any one of cross-sections cut by XY, YZ and XZ planes of any one of rectangular, spherical, triangular, , And the shape of each cross section cut by the XY, YZ, and XZ planes may be the same or different from each other.

According to another preferred embodiment of the present invention, the thickness of the magnetic layer may be 50 to 200 nm.

According to another preferred embodiment of the present invention, the thickness of the magnetic layer may be 100 to 200 nm.

In addition, the present invention provides a method for controlling the above-mentioned magnetic field controllable body support by an external magnetic field to move to a target position in the living body.

Further, the present invention relates to a method for producing a porous bio-scaffold; And coating a magnetic material on the prepared bio-scaffold. The present invention also provides a method of manufacturing a bio-scaffold capable of controlling a magnetic field.

According to a preferred embodiment of the present invention, the step of preparing the bio-support may include the steps of: preparing a bio-scaffold of a porous bio-support by a lithography method using a photocurable polymer; have.

According to another preferred embodiment of the present invention, the step of coating the magnetic material may further include coating a biocompatible metal on the coated magnetic material.

According to another preferred embodiment of the present invention, there is provided a method of manufacturing a biomedical device, comprising the steps of: after the step of coating the biocompatible metal, culturing the cells in the pores of a metal-coated magnetic field controllable biological support; As shown in FIG.

According to another preferred embodiment of the present invention, the three-dimensional body support may be cylindrical, hexahedral, elliptical, polyhedral or conical.

The movable body support capable of controlling the magnetic field of the present invention has excellent biocompatibility and can control the movement of the living body support by an external magnetic field to easily reach a target position. Therefore, it is not necessary to insert directly into the transplantation site with the help of surgical methods and machines, and it is possible to easily place the living body support even in a dangerous part when exposed to the outside such as the local area, blood vessel and brain tissue.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a process diagram illustrating a manufacturing process of a movable body support capable of controlling a magnetic field according to a preferred embodiment of the present invention. FIG.
FIG. 2 is a SEM photograph of a magnetic field controllable mobile biocompound prepared according to the method of Example 1 or Example 2. FIG.
3 is an SEM photograph of an enlarged view of a movable body support capable of controlling a magnetic field produced according to the method of Example 1. Fig.
4 is an SEM photograph of an enlarged view of a movable body support capable of controlling a magnetic field produced according to the method of Example 2. Fig.
5 is a photograph showing a state where a cell is cultured on a mobile body support capable of controlling a magnetic field according to the method of Example 3. Fig.
6 is an image showing a state of control by an external magnetic field of a mobile body support capable of controlling a magnetic field according to a preferred embodiment of the present invention.

Hereinafter, the present invention will be described in more detail with reference to the accompanying drawings.

As described above, the conventional biofilm has to be directly inserted and fixed at a desired position with the help of a surgical method and a machine in order to insert the biofilm in a living body and place it at a transplantation site. In addition, there is a problem in that it is difficult to apply the method to a dangerous part when exposed to external parts such as a local site or a blood vessel or a brain tissue which is difficult to access.

Accordingly, the present invention provides a porous bio-scaffold; And a magnetic body layer coated on the living body support; And a bio-scaffold capable of controlling the magnetic field, which comprises the above-mentioned bio-scaffold.

By this, it is possible to control the movement of the living body support by the external magnetic field while having excellent biocompatibility, and to easily reach the target position.

The magnetic field-controllable movable bio-scaffold of the present invention can be moved to a desired position by an external magnetic field by coating a magnetic material on a porous bio-support to form a magnetic body layer.

First, the porous biocompatible material is not particularly limited as long as it can be used as a conventional biocompatible material. The porous biocompatible material may be a polymer, a ceramic, a nanofiber, a phospholipid layer or a biocompatible metal. Dimensional structure.

Preferably, the porous bio-support may have a three-dimensional structure including a photocurable polymer, and the three-dimensional structure may advantageously support cell migration and invasion. Further, more preferably, the multi-symbiotic living body support is manufactured by a lithography method using a photocurable polymer, so that a micro-sized three-dimensional structure biological living body support capable of easily moving in vivo can be formed.

The photocurable polymer is a polymer that cures when irradiated with light and is not particularly limited as long as it can form a three-dimensional living body support by lithography. More preferably, SU-8 polymer, KMPR, IP-L or IP -G, and the like, and most preferably SU-8 polymer.

 The three-dimensional bio-scaffold formed in this manner can suitably control its shape depending on cells and tissues to be transplanted and is not particularly limited, but may be more preferably cylindrical, hexahedral, elliptical, polyhedral, or conical.

In addition, the three-dimensional body support may additionally have two forms of a cylindrical shape, a hexahedral shape, an elliptical spherical shape, a polyhedral shape, or a conical shape. For example, a three-dimensional living body support or a hexahedral type in which two types of cylindrical shape and helical shape are joined, and two types of hexahedron type in which a wavy shape or a longitudinal length is narrower than the above one type are bonded A three-dimensional bio-scaffold of the present invention can be produced.

Further, on the XYZ coordinate system of the 3D living body support, any one of the cross sections cut by the XY, YZ, and XZ planes may be a sphere, a square, or a triangle.

According to a preferred embodiment of the present invention, when the 3D living body scaffold has a spherical shape in which one of the XY, YZ, and XZ planes is spherical, the area of the 3D living body support is 3.14 to 785,000 탆 ² .

According to another preferred embodiment of the present invention, when any one of the cross sections cut by the XY, YZ, and XZ planes on the XYZ coordinate of the 3D living body support is a square shape, the area of the cross section is 1 to 10 ⁶ 탆 ² < / RTI >

According to another preferred embodiment of the present invention, when the three-dimensional body scaffold is triangular in cross-section by XY, YZ and XZ planes on the XYZ coordinate, the cross-sectional area is 0.5 to 5 * 10 ⁵ 탆 ² .

According to another preferred embodiment of the present invention, the shapes of the cross sections cut by the XY, YZ and XZ planes may be the same or different from each other.

Further, the size of the three-dimensional body scaffold is not particularly limited as long as it can be inserted and moved in a living body, but it may preferably have a length, a length or a diameter of 1 to 1,000 μm and a height of 1 to 1,000 μm More preferably 10 to 300 mu m in width, length or diameter, and 10 to 300 mu m in height. If the living body support satisfies the above range, it can be easily moved in vivo by an external magnetic field, and is advantageous for swimming in blood vessels, ventricles, viscoelastic properties in the organ, fluid and the like. For example, in the case of the cylindrical form of Example 1, the diameter was 75 탆 and the height was 150 탆, and in the case of the hexahedron of Example 2, the width was 75 탆, the length was 75 탆, and the height was 150 탆 .

In addition, the pore size of the porous biocompatible material can be controlled depending on the cells and tissues implanted in the biocompatible material, and there is no particular limitation thereto. Preferably from 5 to 30 mu m, and more preferably from 10 to 20 mu m.

The present invention includes a magnetic body layer coated on such a porous biological support. It is possible to control the movement of the living body support by the external magnetic field by coating the magnetic substance on the living body support so that the living body support is positioned at the implantation site without directly inserting the living body support at the implantation site with the help of the surgical method or machine . If the magnetic material is not coated on the porous bio-support and magnetic particles are contained in the bio-support, it is difficult to control by the external magnetic field, and it is difficult to form the bio-support of the three-dimensional structure using the lithography method . Since magnetic particles are not transparent to light, interference is increased when light is irradiated. Therefore, it is difficult to form a desired three-dimensional body scaffold by lithography, irregular shape of a living body scaffold can be formed irregularly, And the array is also irregularly formed. In order to produce a micro-sized three-dimensional bio-scaffold which can be easily moved in vivo by an external magnetic field, lithography is preferable, and therefore, it is not suitable to prepare a bio-scaffold including magnetic particles.

The coated magnetic material layer is not particularly limited as long as it is a metal having a magnetic property and a small corrosivity (reactivity), but is preferably a metal such as nickel (Ni), iron (Fe), cobalt (Co), or neodymium (Nd) Or mixed, and most preferably nickel (Ni).

The thickness of the magnetic material layer is not particularly limited as long as the thickness of the living body support has a magnetic property capable of moving in vivo by an external magnetic field, but may be preferably 50 to 200 nm, more preferably 100 to 200 nm . If the thickness of the magnetic substance layer is less than 50 nm, there is a problem that the magnetic property is insufficient and control of the in vivo support is difficult due to the external magnetic field. When the thickness exceeds 200 nm, it takes a long time to deposit the magnetic substance layer, The surface of the living body support is not smooth, the magnetic substance is not uniformly deposited on the photo-curable polymer, and the thickly formed magnetic substance layer may be separated from the photo-curable polymer as a whole.

Furthermore, the magnetic field controllable biological support may further comprise a biocompatible metal layer coated on the coated magnetic layer to improve in vivo stability and biocompatibility.

The biocompatible metal layer is not particularly limited as long as it is stable in the living body and is excellent in biocompatibility. It is more preferable that the biocompatible metal layer is composed of titanium (Ti), medical stainless steel, alumina (Al ₂ O ₃ ) Or mixed, and most preferably titanium (Ti).

The thickness of the biocompatible metal layer is not particularly limited as long as it does not deteriorate the biocompatibility of the biocompatible support, but it may preferably be 10 to 50 nm. If the thickness of the biocompatible metal layer is less than 10 nm, the biocompatibility is significantly reduced. When the thickness exceeds 50 nm, it takes a long time to deposit the biocompatible metal layer and the biocompatible metal layer is not separated cleanly from the substrate. There is a problem that the surface is not smooth and the biocompatible metal is not uniformly deposited on the magnetic body.

Such a magnetic field controllable biological support may comprise cells cultured in the pores. The cell-support complex can be formed by culturing the same or different cells in the pores of the magnetic field controllable body support, and the cell-support complex can be injected into the living body and controlled by the external magnetic field to move to the implantation site.

In addition, the present invention provides a method for moving the above-mentioned movable body support capable of controlling a magnetic field by an external magnetic field to a target position in the living body.

FIG. 6 shows a magnetic field control of a movable body support capable of controlling a magnetic field of the present invention. As shown in FIG. 2, a movable body support capable of controlling a magnetic field is subjected to a linear motion (A: linear motion principle, C: (E: simultaneous control, F: target point tracking) is performed on a three-dimensional complex path by a combination of these motions, . Therefore, it is unnecessary to directly insert a living body support into a graft site with the help of surgical methods and machines as in the past, and there is little risk of infection and trauma, and even when exposed to the outside such as a local area, The biological support can be easily positioned through the magnetic field control.

The present invention also relates to a method of manufacturing a bio-scaffold of a porous bio-scaffold; And coating a magnetic material on the prepared bio-scaffold. The present invention also provides a method of manufacturing a bio-scaffold capable of controlling a magnetic field.

Specifically, the step of preparing a porous biocompatible support will be described.

Porous bio-supports may be prepared by conventional methods for preparing various bio-supports, for example, particulate leaching, emulsion freeze-drying, high- pressure gas expansion and phase separation, Fused Deposition Modeling (FDM), and lithography.

The material for preparing the porous biocompatible material is not particularly limited as long as it can be used as a conventional biocompatible material and can be made of polymer, ceramic, nanofiber, phospholipid layer or biocompatible metal, May form a three-dimensional structure as well as a two-dimensional structure.

In the present invention, in order to produce a porous bio-support which is easy to move in vivo by external magnetic field control, a three-dimensional bio-scaffold is manufactured by a lithography method using a photocurable polymer . The three-dimensional structure is advantageous in supporting migration and infiltration of cells, and can be produced by a lithography method using a photocurable polymer. Thus, a micro-sized three-dimensional porous biocompatible Can be manufactured.

The photocurable polymer is a polymer that cures when irradiated with light and is not particularly limited as long as it can form a three-dimensional living body support by lithography. More preferably, SU-8 polymer, KMPR, IP-L or IP -G, and the like, and most preferably SU-8 polymer.

The 3-dimensional bio-scaffold prepared in this manner can be suitably regulated in accordance with the cells and tissues to be transplanted and is not particularly limited, but it is more preferable that it is made of a cylindrical, hexahedral, elliptical spherical, polyhedral or conical shape .

In addition, the three-dimensional body scaffold may have a sphere, a quadrangle, or a triangle on either side of the XYZ, XZ, YZ, and XZ planes cut out by the XYZ coordinate system.

According to a preferred embodiment of the present invention, when the 3D living body scaffold has a spherical shape in which one of the XY, YZ, and XZ planes is spherical, the area of the 3D living body support is 3.14 to 785,000 탆 ² .

According to another preferred embodiment of the present invention, when any one of the cross sections cut by the XY, YZ, and XZ planes on the XYZ coordinate of the 3D living body support is a square shape, the area of the cross section is 1 to 10 ⁶ 탆 ² < / RTI >

According to another preferred embodiment of the present invention, when the three-dimensional body scaffold is triangular in cross-section by XY, YZ and XZ planes on the XYZ coordinate, the cross-sectional area is 0.5 to 5 * 10 ⁵ 탆 ² .

According to another preferred embodiment of the present invention, the porous bio-scaffold is any one of cross-sections cut by XY, YZ and XZ faces is a sphere, a rectangle, a triangle or a polygon having five or more sides, The shapes of the cross sections cut by the XY, YZ, and XZ planes may be the same or different from each other.

Further, the size of the three-dimensional body scaffold is not particularly limited as long as it can be inserted and moved in a living body, but it may preferably have a length, a length or a diameter of 1 to 1,000 μm and a height of 1 to 1,000 μm More preferably 10 to 300 mu m in width, length or diameter, and 10 to 300 mu m in height. If the living body support satisfies the above range, it can be easily moved in vivo by an external magnetic field, and is advantageous for swimming in blood vessels, ventricles, viscoelastic properties in the organ, fluid and the like.

In addition, the pore size of the porous biocompatible material can be controlled depending on the cells and tissues implanted in the biocompatible material, and there is no particular limitation thereto. Preferably from 5 to 30 mu m, and more preferably from 10 to 20 mu m.

The size and the pore size of the three-dimensional body scaffold can be adjusted to satisfy the above range by controlling the intensity of light, scan speed, slice distance, and the like, which are examined in the lithography method.

Next, a magnetic material is coated on the prepared living body support.

It is possible to control the movement of the living body support by the external magnetic field by coating the magnetic substance on the living body support so that the living body support is positioned at the implantation site without directly inserting the living body support at the implantation site with the help of the surgical method or machine . If the magnetic material is prepared not including the magnetic material on the prepared porous bio-support, but the magnetic particles are included in the preparation of the bio-support, it is difficult to control by the external magnetic field, and the three- There is a difficulty in forming. Since the magnetic particles are not transparent to light, interference is increased when light is irradiated. Therefore, it is difficult to form a desired three-dimensional body scaffold by the lithography method. Thus, the shape of the living body scaffold can be irregularly formed, There is a problem that the size and the arrangement of the electrodes are irregularly formed. In order to produce a micro-sized three-dimensional bio-scaffold which can be easily moved in vivo by an external magnetic field, lithography is preferable, and therefore, it is not suitable to prepare a bio-scaffold including magnetic particles.

 The magnetic material to be coated is not particularly limited as long as it is a metal having a magnetic property and a small corrosivity (reactivity), but is preferably a metal such as nickel (Ni), iron (Fe), cobalt (Co) or neodymium Or mixed, and most preferably nickel (Ni).

The method of coating the magnetic material on the prepared porous bio-support is not particularly limited as long as it is a conventional coating method, and more preferably, the electron-beam deposition method, the dipping method, the electrolytic plating method, the sputtering method or the chemical vapor deposition method By weight.

The thickness of the magnetic material layer formed by coating the magnetic material is not particularly limited as long as the thickness of the living body support has magnetism capable of moving in vivo by an external magnetic field, but it may preferably be 50 to 200 nm, Lt; RTI ID = 0.0 > nm. ≪ / RTI > If the thickness of the magnetic substance layer is less than 50 nm, there is a problem that the magnetic property is insufficient and control of the in vivo support is difficult due to the external magnetic field. When the thickness exceeds 200 nm, it takes a long time to deposit the magnetic substance layer, The surface of the living body support is not smooth, the magnetic substance is not uniformly deposited on the photo-curable polymer, and the thickly formed magnetic substance layer may be separated from the photo-curable polymer as a whole.

Further, the method for manufacturing a movable body support capable of controlling the magnetic field may further include coating a biocompatible metal on the coated magnetic material to improve stability and biocompatibility in vivo.

The biocompatible metal is not particularly limited as long as it is stable in vivo and has excellent biocompatibility. More preferably, the biocompatible metal is a metal such as titanium (Ti), medical stainless steel, alumina (Al ₂ O ₃ ) Or mixed, and most preferably titanium (Ti).

As with the coating of the magnetic material, the coating method is not particularly limited as long as it is a conventional coating method, but it can be more preferably coated by an electron beam deposition method, a dipping method, an electrolytic plating method, a sputtering method or a chemical vapor deposition method.

The thickness of the biocompatible metal layer formed by coating the biocompatible metal is not particularly limited as long as the biocompatibility of the biocompatible material is not impaired, but may be preferably 10 to 50 nm. If the thickness of the biocompatible metal layer is less than 10 nm, the biocompatibility is significantly reduced. When the thickness exceeds 50 nm, it takes a long time to deposit the biocompatible metal layer and the biocompatible metal layer is not separated cleanly from the substrate. There is a problem that the surface is not smooth and the biocompatible metal is not uniformly deposited on the magnetic body.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples should not be construed as limiting the scope of the present invention, and should be construed to facilitate understanding of the present invention.

< Example 1 > Manufacture of Cylindrical Magnetic Field Controlled Mobile Body Support

A 3 cm glass wafer was washed in an ultrasonic bath using isopropyl alcohol (IPA) to remove residual dust and organic materials. 1.25 mL of SU-8 (NANOTM SU-8 100, Microchem) was spin-coated at a rate of 100 rpm / s for 10 seconds of ramp up to 500 rpm and 300 rpm / s for 30 seconds of ramp up to 1000 rpm . From this process, a 100 탆 thick SU-8 layer was coated on the glass. After that, the mixture was treated on a 65 ° C hot plate for 10 minutes, then at 95 ° C for 30 minutes, and then at room temperature for 10 minutes.

Two-photon polymerization (TPP) 3D laser lithography was then performed to polymerize the designed structures. The laser intensity, scan speed, and slice distance were 16% intensity, 50 ㎛ / s, and 0.9 ㎛ of maximum laser intensity (20 ㎽ / sec). Then treated on a 65 ° C hot plate for 1 minute, and at 95 ° C for 10 minutes.

After cooling the sample, a cylindrical porous support having a diameter of 75 mu m, a height of 150 mu m and a pore size of 10 to 20 mu m was prepared using mr-Dev 600 for 20 minutes to develop SU-8 .

A multilayer of a magnetic body layer (Ni) and a biocompatible metal layer (Ti) was formed on the support. First, 150 nm of nickel (Ni), which is a magnetic material layer, was coated using an E-beam evaporation system in which a chuck was tilted for uniform deposition. Then, a biocompatible metal layer of 20 占 퐉 in thickness was coated on the bio-support on which the magnetic layer was laminated.

SEM photographs of the thus prepared movable bio-scaffold capable of controlling the magnetic field are shown in FIG.

< Example 2 > Hexahedral type  Fabrication of magnetic field-controlled mobile bio-support

The laser intensity, scan speed and slice distance were set to 16% of the laser intensity (20 ㎽ / sec), 50 ㎛ / s and 0.9 ㎛, and the transverse length was 75 ㎛, Mu] m and a pore size of 10 to 20 nm was prepared in the same manner as in Example 1, except that a biocompatible porous support was prepared.

FIG. 4 shows an SEM photograph of the thus-prepared movable body support capable of controlling the magnetic field.

< Example 3 > Cell culture in magnetic field control mobile biomaterial support

Prior to culturing the cells, the bio-scaffolds prepared on glass were immersed in an autoclave at 10 [mu] m / ml of poly L lysine (PLL) (0.01%; Sigma Chemical Co., St. Louis, Mo., USA).

HEK293 (human embryonic kidney 293) cells to be cultured in the above-mentioned biocompatible cells were cultured in Dulbecco's Modification of Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (SH30919.03, Thermo, USA) And cultured at 37 ° C, 5% CO ₂ . The medium was then discarded and ethylenediaminetetra acetic acid (EDTA), containing 0.25% (wt / vol) trypsin, was added to prepare a 1x106 cells / ml cell suspension. The cells were then treated in an incubator at 37 ° C for 30 seconds and centrifuged at 1000 rpm for 5 minutes to separate the cells and DMEM.

The cell supernatant separated from the above was cultured in a medium containing the magnetic field controllable mobile body support. The cells of the medium were cultured for 4 days, and after 4 days, the medium was removed and the dish was washed with distilled phosphate buffered solution (dPBS). The cells were then impregnated with 4% paraformaldehyde solution and placed at 4 ° C for 24 hours. The paraformaldehyde was then removed and the surface of the glass wafer was washed three times with dPBS. After clearing the Dish, the cells were coated and platinum plated onto the cells to observe the cell cultured magnetic field controllable mobile support by SEM.

FIG. 5 shows the result of culturing the cells on a mobile body support capable of controlling the magnetic field. The HEK293 cells used stuck to the living body support, and it was found that the magnetic field controllable living body support of the present invention is biocompatible when viewed from the filopodia extending from the cells.

Claims (7)

Wherein one of the cross sections cut by the XY, YZ, and XZ planes on the XYZ coordinate is any one of a sphere, a rectangle, a triangle, or a polygon having five or more sides, and a pore having a size of 5 to 30 탆 A three-dimensional three-dimensional porous bio-scaffold; And
A magnetic substance layer coated on the surface of the living body support to a thickness of 50 to 200 nm so that a living body support inserted into the living body by a magnetic field provided from the outside can be moved to a desired position;
A biocompatible metal layer coated on the surface of the magnetic layer at a thickness of 10 to 50 nm; / RTI &gt;
The magnetic layer may be formed by coating at least one of nickel (Ni), iron (Fe), cobalt (Co), and neodymium (Nd) by an E-beam evaporation system,
Wherein the biocompatible metal layer is coated with at least one of titanium (Ti), stainless steel, alumina (Al2O3) or gold (Au) by an E-beam evaporation system (bio-scaffold). delete 2. The movable body support as claimed in claim 1, comprising cells cultured in the pores of the magnetic field controllable living body support. delete delete delete The method according to claim 1,
Wherein the magnetic body layer has a thickness of 100 to 200 nm.

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