KR101552819B1 - Method for Maximizing antioxidant of Formica truncicola having treatment effect for prostate hypertrophy, urinary incontinence and premature ejaculation - Google Patents
Method for Maximizing antioxidant of Formica truncicola having treatment effect for prostate hypertrophy, urinary incontinence and premature ejaculation Download PDFInfo
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- KR101552819B1 KR101552819B1 KR1020150086266A KR20150086266A KR101552819B1 KR 101552819 B1 KR101552819 B1 KR 101552819B1 KR 1020150086266 A KR1020150086266 A KR 1020150086266A KR 20150086266 A KR20150086266 A KR 20150086266A KR 101552819 B1 KR101552819 B1 KR 101552819B1
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- Prior art keywords
- powder
- truncicola
- formica
- extract
- ethanol
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- 230000003078 antioxidant effect Effects 0.000 title claims abstract description 13
- 238000000034 method Methods 0.000 title claims abstract description 13
- 241001251054 Formica truncorum Species 0.000 title abstract description 10
- 230000000694 effects Effects 0.000 title description 10
- 206010046543 Urinary incontinence Diseases 0.000 title description 3
- 206010036596 premature ejaculation Diseases 0.000 title description 3
- 210000002307 prostate Anatomy 0.000 title description 3
- 206010020880 Hypertrophy Diseases 0.000 title description 2
- 239000003963 antioxidant agent Substances 0.000 title 1
- 239000000843 powder Substances 0.000 claims abstract description 47
- 239000000469 ethanolic extract Substances 0.000 claims abstract description 39
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 26
- 238000001914 filtration Methods 0.000 claims abstract description 24
- 239000000284 extract Substances 0.000 claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 108091005804 Peptidases Proteins 0.000 claims abstract description 14
- 238000002156 mixing Methods 0.000 claims abstract description 14
- 102000035195 Peptidases Human genes 0.000 claims abstract description 13
- 239000008213 purified water Substances 0.000 claims abstract description 13
- 238000004108 freeze drying Methods 0.000 claims abstract description 12
- 235000021067 refined food Nutrition 0.000 claims abstract description 8
- 241000257303 Hymenoptera Species 0.000 claims description 18
- 241000254158 Lampyridae Species 0.000 claims description 16
- 230000000845 anti-microbial effect Effects 0.000 claims description 16
- 239000004599 antimicrobial Substances 0.000 claims description 14
- 239000000243 solution Substances 0.000 claims description 14
- 239000012670 alkaline solution Substances 0.000 claims description 11
- 239000000203 mixture Substances 0.000 claims description 10
- 210000003056 antler Anatomy 0.000 claims description 5
- 239000011888 foil Substances 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 16
- 239000007864 aqueous solution Substances 0.000 abstract description 7
- 239000004365 Protease Substances 0.000 abstract description 2
- 238000000926 separation method Methods 0.000 abstract 1
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 14
- 229940121375 antifungal agent Drugs 0.000 description 10
- 230000000843 anti-fungal effect Effects 0.000 description 8
- 230000002421 anti-septic effect Effects 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 8
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 7
- 235000019253 formic acid Nutrition 0.000 description 7
- 230000002292 Radical scavenging effect Effects 0.000 description 6
- 239000003513 alkali Substances 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 4
- 239000008176 lyophilized powder Substances 0.000 description 4
- 238000001694 spray drying Methods 0.000 description 4
- 102000019197 Superoxide Dismutase Human genes 0.000 description 3
- 108010012715 Superoxide dismutase Proteins 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 241000894007 species Species 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- GLKCOBIIZKYKFN-UHFFFAOYSA-N 2-aminopteridine-4,7-diol Chemical compound N1=CC(O)=NC2=NC(N)=NC(O)=C21 GLKCOBIIZKYKFN-UHFFFAOYSA-N 0.000 description 2
- ASJSAQIRZKANQN-CRCLSJGQSA-N 2-deoxy-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)CC=O ASJSAQIRZKANQN-CRCLSJGQSA-N 0.000 description 2
- 206010057190 Respiratory tract infections Diseases 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 239000003429 antifungal agent Substances 0.000 description 2
- 229910052787 antimony Inorganic materials 0.000 description 2
- WATWJIUSRGPENY-UHFFFAOYSA-N antimony atom Chemical compound [Sb] WATWJIUSRGPENY-UHFFFAOYSA-N 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000007602 hot air drying Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 235000012149 noodles Nutrition 0.000 description 2
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 2
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 2
- WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 208000006820 Arthralgia Diseases 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- 244000299461 Theobroma cacao Species 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 229930003471 Vitamin B2 Natural products 0.000 description 1
- 235000013334 alcoholic beverage Nutrition 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000002072 anti-mutant effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 229940064004 antiseptic throat preparations Drugs 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 206010006451 bronchitis Diseases 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000019219 chocolate Nutrition 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 210000000232 gallbladder Anatomy 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 235000015243 ice cream Nutrition 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 235000013550 pizza Nutrition 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 235000011056 potassium acetate Nutrition 0.000 description 1
- 229910000027 potassium carbonate Inorganic materials 0.000 description 1
- 235000011181 potassium carbonates Nutrition 0.000 description 1
- 239000001508 potassium citrate Substances 0.000 description 1
- 229960002635 potassium citrate Drugs 0.000 description 1
- QEEAPRPFLLJWCF-UHFFFAOYSA-K potassium citrate (anhydrous) Chemical compound [K+].[K+].[K+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QEEAPRPFLLJWCF-UHFFFAOYSA-K 0.000 description 1
- 235000011082 potassium citrates Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229940079877 pyrogallol Drugs 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 235000013580 sausages Nutrition 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 235000011888 snacks Nutrition 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 235000011083 sodium citrates Nutrition 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 230000002485 urinary effect Effects 0.000 description 1
- 231100000611 venom Toxicity 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011716 vitamin B2 Substances 0.000 description 1
- 235000019164 vitamin B2 Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A23L1/30—
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
- A61K35/64—Insects, e.g. bees, wasps or fleas
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/204—Animal extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/14—Extraction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Insects & Arthropods (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Animal Husbandry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
(A) immersing and drying the dried firefly in an aqueous alkaline solution; (b) adding an aqueous ethanol solution to the firewood taken out in step (a), extracting it, filtering and separating the ethanol extract; (c) separating the ethanol extract of step (b) and adding purified water and proteolytic enzyme to the remaining antiserum extract foil; And (d) mixing the extracted extract of step (c) with the ethanol extract of step (b), filtering and freeze-drying the antifungal powder. , An antimicrobial powder prepared by the above method, and a method for promoting antioxidative activity of a processed food containing the antimicrobial powder and an antimicrobial powder.
Fireworks ( Formica truncicola ) is a species of ants belonging to the ants. The body has a dark red color and a light yellow hairs throughout the body. Fire ants live in rotten trees, soil, and stones in Korea, Japan, China, Taiwan, and Russia, and do not live under live trees. Fireworms are widely distributed inland in Korea and fewer in the south. Currently, there are about 60 species of ants in 1, 26, and 60 species including ants and fire ants in Korea.
The efficacy of fire antibiotics is known to be effective for joint pain, cardiovascular disease, respiratory system bronchitis, urinary system, digestive system, nervous system. 100 grams of dried ants contain over 70 grams of protein and contain 28 free amino acids and 8 amino acids, including isoxanthopterin, biotin, vitamin B2, 2-amino-6-hydro And cyperteridine.
The ant ingredient contains a venomous solution in the gall bladder, the most important of which is formic acid, and contains 90% of the antialdehyde. The antimutant is known to have the above nutritional elements and efficacy, but the formic acid which shows the toxicity of fire ants is strong enough to be 20 times that of Japanese royal family, and it is difficult to completely neutralize the formic acid in general processing, The development of processed food using
Korean Patent Laid-Open Publication No. 2004-0023459 discloses a known technology for manufacturing health supplement foods by mixing fire antiseptics with various medicines and Korean Patent No. 1136090 discloses an antifungal agent A functional food containing a liquid obtained by heating after mixing a medicinal herb is known, but is different from the method for producing an antimicrobial powder in which the antioxidative activity of the present invention is enhanced.
The present invention has been made in view of the above-mentioned needs, and it is an object of the present invention to provide an antifungal agent which can effectively neutralize formic acid contained in an antler, thereby solving the problems of human toxicity during ingestion, It is an object of the present invention to establish a manufacturing method of an antimicrobial powder having an antioxidant activity enhanced by extracting fire ants and then lyophilization.
In order to solve the above-described problems, the present invention provides a method for producing a firefly, comprising the steps of: (a) immersing a dried firefly in an aqueous alkaline solution and then taking it out; (b) adding an aqueous ethanol solution to the firewood taken out in step (a), extracting it, filtering and separating the ethanol extract; (c) separating the ethanol extract of step (b) and adding purified water and proteolytic enzyme to the remaining antiserum extract foil; And (d) mixing the extracted extract of step (c) with the ethanol extract of step (b), filtering and freeze-drying the antifungal powder. .
Further, the present invention provides an antifungal powder produced by the above method.
Further, the present invention provides a processed food containing the above antimicrobial powder.
Further, the present invention provides a method for producing a firefly, comprising: (a) immersing a dried firefly in an aqueous alkaline solution and then taking it out; (b) adding an aqueous ethanol solution to the firewood taken out in step (a), extracting it, filtering and separating the ethanol extract; (c) separating the ethanol extract of step (b) and adding purified water and proteolytic enzyme to the remaining antiserum extract foil; And (d) mixing the extracted extract of step (c) with the ethanol extract of step (b), filtering and freeze-drying the antifungal powder.
The present invention is advantageous in that the antioxidative activity is improved by efficiently extracting the functional ingredient contained in the antimicrobial agent while effectively neutralizing the formic acid contained in the antimicrobial agent through the antimicrobial pretreatment process and lyophilizing the extracted extract to powder, There is an effect that the powder can be usefully used in foods, medicines, cosmetics industry, and the like.
In order to achieve the object of the present invention,
(a) immersing the dried firefly in an aqueous alkaline solution and then taking it out;
(b) adding an aqueous ethanol solution to the firewood taken out in step (a), extracting it, filtering and separating the ethanol extract;
(c) separating the ethanol extract of step (b) and adding purified water and proteolytic enzyme to the remaining antiserum extract foil; And
(d) mixing the extracted extract of step (c) with the ethanol extract of step (b), filtering and freeze-drying the antifungal powder. to provide.
In the method for producing an antimicrobial powder of the present invention, the aqueous alkali solution of step (a) may be an aqueous 0.1-10 wt% alkali solution, preferably a pH 8-9 aqueous solution, more preferably a 0.1 wt% Lt; / RTI > The alkali used in the aqueous alkali solution is preferably at least one selected from the group consisting of sodium bicarbonate, sodium acetate, potassium acetate, sodium citrate, potassium citrate and potassium carbonate, more preferably sodium bicarbonate . Through the alkali treatment as described above, the formic acid contained in the fire ants could be partially neutralized.
In addition, in the method for producing an antidandruff powder of the present invention, the protease of step (c) may be but is not limited to Alkalase.
Further, in the method for producing an antidandruff powder of the present invention, the freeze-drying in the step (d) is preferably freeze-dried at -50 to -60 ° C for 20 to 28 hours, more preferably at -55 ° C It can be lyophilized for 24 hours. It was possible to produce an antimicrobial powder having improved physiological activity effects such as antioxidative activity and the like, compared to drying the pre-treated antimicrobial agent under other conditions and conditions.
The method for producing an antimicrobial powder of the present invention, more specifically,
(a) immersing the dried firefly in an aqueous alkaline solution having a pH of 8 to 9 for 50 to 70 minutes and then taking out the same;
(b) adding an aqueous 45 to 55% (v / v) ethanol solution to the fire ants taken in step (a), extracting the mixture at 50 to 70 ° C for 8 to 12 hours, filtering and separating the ethanol extract;
(c) separating the ethanol extract of step (b) and adding purified water and proteolytic enzyme to the remaining firewood, and maintaining the pH at 8 to 9 and extracting at 40 to 50 ° C for 65 to 80 hours; And
(d) mixing the extracted extract of step (c) with the ethanol extract of step (b), filtering and then freeze-drying at -50 to -60 ° C for 20 to 28 hours,
More specifically,
(a) immersing the dried firefly in an aqueous alkaline solution of pH 8.5 for 60 minutes and then taking it out;
(b) adding 50% (v / v) ethanol aqueous solution to the fire ants taken in step (a), extracting at 60 ° C for 10 hours, filtering and separating the ethanol extract;
(c) separating the ethanol extract from the step (b), adding purified water and proteolytic enzyme to the remaining antler leaves, and extracting the mixture at 40 to 50 ° C for 72 hours while maintaining the pH at 9; And
(d) mixing the extracted extract of step (c) with the ethanol extract of step (b), filtering, and lyophilizing at -55 ° C for 24 hours.
In the method of manufacturing an antimicrobial powder of the present invention, the antimicrobial pretreated through the steps (a) to (c) above effectively removes toxic substances such as formic acid and enhances extraction efficiency, And could be prepared as an enhanced extract.
The present invention also provides an antimicrobial powder improved in antioxidative activity produced by the above method.
The present invention also provides a processed food containing the fire antler powder. There is no particular limitation on the kind of the processed food. Examples of the foods to which the antiseptic powder can be added include dairy products including soups, meats, sausages, breads, chocolates, candies, snacks, confectionery, pizza, ramen noodles, other noodles, gums, ice cream, , A drink, an alcoholic beverage, and a vitamin complex, all of which are processed foods in a conventional sense.
The present invention also relates to
(a) immersing the dried firefly in an aqueous alkaline solution having a pH of 8 to 9 for 50 to 70 minutes and then taking out the same;
(b) adding an aqueous 45 to 55% (v / v) ethanol solution to the fire ants taken in step (a), extracting the mixture at 50 to 70 ° C for 8 to 12 hours, filtering and separating the ethanol extract;
(c) separating the ethanol extract of step (b) and adding purified water and proteolytic enzyme to the remaining firewood, and maintaining the pH at 8 to 9 and extracting at 40 to 50 ° C for 65 to 80 hours; And
(d) mixing the extracted extract of step (c) with the ethanol extract of step (b), filtering, and lyophilizing at -50 to -60 ° C for 20 to 28 hours. / RTI >
The method for enhancing the antioxidant activity of the antifungal powder of the present invention is more specifically
(a) immersing the dried firefly in an aqueous alkaline solution of pH 8.5 for 60 minutes and then taking it out;
(b) adding 50% (v / v) ethanol aqueous solution to the fire ants taken in step (a), extracting at 60 ° C for 10 hours, filtering and separating the ethanol extract;
(c) separating the ethanol extract from the step (b), adding purified water and proteolytic enzyme to the remaining antler leaves, and extracting the mixture at 40 to 50 ° C for 72 hours while maintaining the pH at 9; And
(d) mixing the extracted extract of step (c) with the ethanol extract of step (b), filtering, and lyophilizing at -55 ° C for 24 hours.
Hereinafter, the present invention will be described in detail with reference to examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.
Manufacturing example 1: Manufacture of freeze-dried lyophilized powder
(1) 1 kg of dry fire ants were immersed in a 0.1% aqueous solution of sodium bicarbonate of pH 8.5 for 1 hour and then recovered.
(2) 5 kg of a 50% ethanol aqueous solution was added to the recovered fireworks, and the mixture was stirred at 60 ° C for 10 hours, filtered, and then the ethanol extract was separated.
(3) 5 kg of purified water was added to the remaining ethanol extract of the above-mentioned antimicrobial extract, and 5 g of proteinase (alkalazine, Novozymes) was added while maintaining the pH at 9 to 40 ° C at 50 ° C. The enzyme was degraded.
(4) The extract of step (3) and the ethanol extract of step (2) were combined, filtered through a 200 mesh microfilter, and lyophilized at -55 ° C for 24 hours to be powdered.
Comparative Example 1: Manufacture of fire antiseptic hot-air dried powder
The powder was prepared by the method of Preparation Example 1, but without lyophilization in the step (4), the powder was dried by hot air at 50 ° C for 6 hours.
Comparative Example 2: Fire ants Spray drying Powder manufacturing
The filtered extract was vacuum-concentrated and adjusted to 30 brix without being lyophilized in step (4), and the filtrate was filtered at a rate of 0.3 L per minute at an inlet temperature of 180 ° C and an air temperature of 110 ° C Followed by spray drying.
Experimental Method
(1) Superoxide dismutase (SOD) -like activity
3 ml of Tris-HCl buffer, 0.2 ml of 0.2 mM pyrogallol and 0.2 ml of a sample were added to the test tube, and the mixture was allowed to stand at 25 ° C for 10 minutes. Then 1 ml of 1N HCl was added to stop the reaction and measure the absorbance at 420 nm.
SOD-like activity (%) = 100 - (A / B * 100)
A: Absorbance of the sample added group
B: Absorbance of the sample without added sample
(2) Hydroxy radical scavenging ability
0.2 ml of 0.1 mM FeSO 4 / EDTA solution, 0.2 ml of 10 mM 2-deoxyribose, 0.1 ml of a sample, 1.3 ml of 0.1 M phosphate buffer (pH 7.4), 10 mM of H 2 O 2 After incubation at 37 ° C for 2 hours, 1 ml of 20% TCA (trichloroacetic acid) was added and the mixture was heated at 100 ° C for 15 minutes, rapidly cooled, and absorbance was measured at 532 nm.
Example 1: Antimony powder SOD Similar activity
SOD-like activity of antifungal powders prepared according to the preparation examples and comparative examples was measured and the results are shown in Table 1 below. As a result, 77.11% of freeze - dried lime - free powder showed higher activity than that of antiseptic hot air drying and spray drying powder.
Example 2: Antimony powder Radical Scatters
Hydroxy radical scavenging activity was measured by 2-deoxyribose oxidation. Hydroxy radicals are known to play an important role in biooxidation due to their strong reactivity in active oxygen. The hydroxy radical scavenging ability of the antifungal powders prepared according to the preparation examples and the comparative examples was measured and the results are shown in Table 2 below. As a result, it showed a radical scavenging ability of 53.13% in the lyophilized lyophilized powder and showed higher scavenging ability than those of 31.01% and 13.41% of the antiseptic hot air drying and spray drying powder.
Claims (6)
(b) adding an aqueous ethanol solution to the firewood taken out in step (a), extracting it, filtering and separating the ethanol extract;
(c) separating the ethanol extract of step (b) and adding purified water and proteolytic enzyme to the remaining antiserum extract foil; And
(d) mixing the extracted extract of step (c) with the ethanol extract of step (b), filtering and lyophilizing the mixture.
(a) immersing the dried firefly in an aqueous alkaline solution having a pH of 8 to 9 for 50 to 70 minutes and then taking out the same;
(b) adding an aqueous 45 to 55% (v / v) ethanol solution to the fire ants taken in step (a), extracting the mixture at 50 to 70 ° C for 8 to 12 hours, filtering and separating the ethanol extract;
(c) separating the ethanol extract of step (b) and adding purified water and proteolytic enzyme to the remaining firewood, and maintaining the pH at 8 to 9 and extracting at 40 to 50 ° C for 65 to 80 hours; And
(d) mixing the extracted extract of step (c) with the ethanol extract of step (b), filtering and then lyophilization at -50 to -60 ° C for 20 to 28 hours. Wherein the antioxidant activity is increased.
(b) adding an aqueous 45 to 55% (v / v) ethanol solution to the fire ants taken in step (a), extracting the mixture at 50 to 70 ° C for 8 to 12 hours, filtering and separating the ethanol extract;
(c) separating the ethanol extract of step (b) and adding purified water and proteolytic enzyme to the remaining firewood, extracting it at 40 to 50 ° C for 65 to 80 hours while maintaining the pH at 8 to 9; And
(d) mixing the extracted extract of step (c) with the ethanol extract of step (b), filtering, and lyophilizing at -50 to -60 ° C for 20 to 28 hours. ≪ / RTI >
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