KR101498506B1 - Pseudomonas koreensis BIG10005 for controlling potato scab and uses thereof - Google Patents

Abstract

The present invention relates to Pseudomonas koreensis BIG10005 having control activity against Streptomyces spp.; a microorganism agent for controlling Streptomyces spp. containing the Pseudomonas koreensis BIG10005 or a culture medium thereof as an active ingredient; a method for controlling Streptomyces spp. comprising a step of treating the microorganism agent in plants or on soil; and a method for preparing the microorganism agent for controlling Streptomyces spp. comprising a step of culturing the Pseudomonas koreensis BIG10005.

Description

Pseudomonas corynsis BIG10005 strain having potato scab disease control effect and its use {Pseudomonas koreensis BIG10005 for controlling potato scab and uses thereof}

The invention potato deodengyi having a disease control effect relates to N-Sys BIG10005 Pseudomonas collection strains and the use thereof, and more particularly, Pseudomonas Corey N-Sys having antifungal activity against potato deodengyi disease (Pseudomonas koreensis BIG10005 strain, a microorganism preparation for controlling potato scab disease comprising the strain or a culture thereof as an active ingredient, a method for controlling potato scab disease comprising treating the microorganism preparation with a plant or soil, and a method for cultivating the strain To a method for producing a microorganism preparation for controlling potato scab disease.

Potatoes ( Solanum tuberosum ) is one of the world's four largest food crops. In 2002, the total production of 25 EU member states was 6,700,000 M / T and the cultivation area was 2,000,000 hectares. In addition, the cultivated area increased by 47% by the new member countries of 10 countries. In Korea, the total production of potatoes was 446.10M / T in 1980, but the production volume increased to 678,305M / T in 1999, and production and consumption are gradually increasing due to western consumption of food. Today, however, damage to crops caused by pests is becoming increasingly serious, especially for potatoes such as Erwinia cartovora , Ralstonia solanacearum , and Streptomyces spp. are known to be the main diseases that cause crops to lose their value.

Streptomyces scabies is a soil-borne disease that allows hospital bacteria to live on soil or plant remnants and can invade the host plants continuously, and the hosts It is known that it can survive for many years in the ground without plants. This disease mainly forms a cork layer of a tuber, which reduces the value of the product rather than the quantity of potatoes. In Korea, the incidence of potato scab disease is on the average 30-40% and in severe cases it is 60-70%. Especially, the quality of seed potatoes is decreased by this disease, and farmers tend to prefer foreign expensive seed potatoes. In addition, in Korea, especially in Jeju Island, which produces edible potatoes, the damage of scab disease is large, but there is no clear control method.

Although the interest in microbial pesticides has been growing as the only means of replacing chemical synthetic pesticides by farmers due to the increase in demand for domestic organic and environmentally friendly agricultural products, microbial pesticides for the control of soil diseases, which are produced and sold in Korea, It is absolutely lacking in comparison. Since the number and types of microorganisms in the soil are various, the resources that can be developed as bio-pesticides are unlimited and the initial investment cost is less than that of chemical pesticide production facilities, It is an industry with a high growth potential.

Korean Patent No. 0563298 discloses a Streptomyces sp. Strain having potato scab disease prevention effect. Korean Patent Publication No. 2006-0012393 discloses a novel strain of Bacillus sp. Inhibiting potato scab disease. Production of antibiotics and microorganism preparations containing them ", but the Pseudomonas corynsis strain BIG10005 having the potato scab disease prevention effect of the present invention and its use are not disclosed.

The present invention has been made in view of the above needs, and the present inventors have completed the present invention by confirming that the Pseudomonas corynsis BIG10005 strain isolated from soil has a remarkable control effect on potato scab disease.

In order to solve the above-mentioned problems, the present invention provides Pseudomonas sp. Having potency control activity against potato scab disease koreensis ) BIG10005 strain.

The present invention also provides a microorganism preparation for controlling potato scab disease comprising the strain or a culture thereof as an active ingredient.

The present invention also provides a method for controlling potato scab disease comprising the step of treating the plant or soil with the microorganism preparation.

The present invention also provides a method for producing a microorganism preparation for controlling potato scab disease comprising the step of culturing the strain.

The present invention provides a new control method that can replace the action of chemical pesticides or chemical fertilizers while being environmentally friendly by providing a method for controlling potato scab disease using a novel Pseudomonas corynsis strain having potency control activity against potato scab disease. Further, the microorganism preparation using the strain of the present invention is expected to be industrially useful because it can be developed as a biological pesticide.

1 is N-Sys Corey Pseudomonas (Pseudomonas koreensis ) BIG10005 strain against Streptomyces scabies .
2 is a phylogenetic diagram showing the phylogenetic location of Pseudomonas corynsis BIG10005 isolated from soil.
Fig. 3 is a photograph of harvested potato cultivated in a soil that does not pretreat or treat Pseudomonas corynsis BIG10005 strain. (a) Potatoes grown in the untreated soil, (b) Potatoes grown in the strain treated soil.

In order to accomplish the object of the present invention, the present invention provides a Pseudomonas koreanis koreensis ) BIG10005 strain.

The Pseudomonas corynsis BIG10005 strain of the present invention was isolated from Pseudomonas sp. Expressing fluorescence from potato cultivation soil and confirmed to have an antagonistic activity against potato scab pathogens. The Pseudomonas corynsis strain BIG10005 was deposited at the Korea Research Institute of Bioscience and Biotechnology on Aug. 30, 2013 (Accession No .: KCTC 12479BP).

The present invention also provides a microorganism preparation for controlling potato scab disease comprising the strain or a culture thereof as an active ingredient.

The microbial preparation for controlling potato scab disease of the present invention comprises Pseudomonas cornisis koreensis strain BIG10005 or a culture thereof. The microorganism preparation for controlling potato scab disease according to the present invention may be prepared as a solution, a powder, a suspension, a dispersion, an emulsion, an oily dispersant, a paste, a dust, a scattering material or a granule But is not limited thereto.

In the microorganism preparation according to the embodiment of the present invention, the pathogen of the potato deodengyi disease Streptomyces Scar bieseu (Streptomyces scabies), Streptomyces ah CD Scar bieseu (Streptomyces acidiscabies) or Streptomyces hair jidi Scar bieseu (Streptomyces turgidiscabies ), preferably Streptomyces scabies , but are not limited thereto.

The present invention also provides a method for controlling potato scab disease comprising the step of treating the plant or soil with a microorganism preparation for controlling potato scab disease.

The microbial preparation for controlling potato scab disease of the present invention comprises Pseudomonas cornisis koreensis strain BIG10005 or a culture thereof, and the microbial agent may be treated, but not limited to, a plant or soil by immersion treatment, crossing treatment, spraying, spraying, spraying, scattering or swelling.

The present invention also provides a method for producing a microorganism preparation for controlling potato scab disease comprising the step of culturing the strain.

The microbial preparation for controlling potato scab disease according to the present invention may be prepared as a solution, a powder, a suspension, a dispersion, an emulsion, an oily dispersant, a paste, a dust, a dispersant or a granule but is not limited thereto.

The composition for controlling plant diseases of the present invention can be formulated into various forms. The preparation may be prepared, for example, by adding a solvent and / or a carrier. Often, inert additives and surface-active materials, such as emulsifiers or dispersants, are mixed into the formulation. Suitable surface-active materials include, but are not limited to, aromatic sulfonic acids (e.g., lignosulfonic acid, phenol-sulfonic acid, naphthalene- and dibutylnaphthalenesulfonic acid), fatty acids, alkyl- and alkylarylsulfonates, alkyl lauryl ethers, A salt of a fatty alcohol glycol ether, a sulfonate naphthalene and derivatives thereof, a condensate of formaldehyde, a condensate of naphthalene or naphthalene sulfonic acid, a phenol and a formaldehyde Condensates, polyoxyethylene octylphenol ethers, ethoxylated isooctyl-, octyl- or nonylphenol, alkylphenyl or tributylphenyl polyglycol ethers, alkylaryl polyether alcohols, isotridecyl alcohol, fatty alcohol / ethylene oxide condensates , Ethoxylated castor oil, polyoxyethylene alkyl ether or polyoxypropylene, lauryl alcohol Polyglycol ether acetate, sorbitol esters, lignin-sulfite waste liquid, or may be a cellulose, but is not limited thereto.

Suitable solid carrier materials are in principle all porous and comprise an agriculturally acceptable carrier such as mineral earths such as silica, silica gel, silicate, talc, kaolin, limestone, lime, chalk, Fertilizers such as ammonium sulfate, ammonium phosphate, ammonium nitrate, urea, vegetable products such as cereal flour, bark powder, wood meal, And nut shell powder), or cellulose powder. The solid carrier may be used alone or in combination of two or more.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Example  1. Isolation and Identification of Antagonistic Strain

In order to obtain antagonistic microorganisms against Streptomyces scabies , Pseudomonas sp. sp.) was isolated pure. The antagonistic ability of the isolated strains was confirmed (FIG. 1) and the genetic fidelity of BIG10005 strains exhibiting antagonistic activity against potato scabbard pathogens was investigated. The 16S DNA sequence was analyzed by DNA extraction and 16S DNA amplification. The amplified PCR product was purified and sequenced. The analyzed nucleotide sequence (1,303 bp) was obtained from the DNA data bank of Japan (DDBJ) / NCBI (US National Bioinformatics Center) / Genebank (http://www.ncbi.nlm.nih.gov/genbank/) and the ribosome database project (www.dict.md) of the bosomal Database Project, and as a result, it was found that a strain belonging to the phylogenetic group including species of Pseudomonas sp. , Pseudomonas corynsis koreensis ) Ps9-14 ^T (AF468452) and was identified as Pseudomonas corynsis (Fig. 2).

Example  2. Pseudomonas Corinisis BIG10005  Biochemical characteristics of strains

In order to investigate the biochemical characteristics of Pseudomonas corynsis strain BIG10005, it was confirmed using an API kit. As a result, Pseudomonas corynsis strain BIG10005 produces an enzyme that degrades protein and gelatin, and is characterized by using L-arabinose, D-galactose, mannose, and inositol Respectively. The biochemical characteristics of the Pseudomonas corynsis strain BIG10005 are summarized in Table 1 below.

Example  3. Pseudomonas Corinisis BIG10005  Effect of potato scab disease control by treatment

Pseudomonas corynsis koreensis ) The control effect of BIG10005 strain on potato scab disease was confirmed in potato cultivation farm in Jeju Island. Before the ceremony for the preparation of the potatoes (Daijima) in Gujurup, Jeju Island, Pseudomonas koelinsis One liter of the BIG10005 strain culture (10 ⁸ cfu / ml) was diluted in 300 L of water, spread on the surface of 200 pyeong soil, tilled, and subjected to rotary working.

Pseudomonas corinisis After 86 days of treatment with strain BIG10005, the area of disease incidence of potato scabbard was examined. As a result, Pseudomonas corynsis The control of potato scab disease in the BIG10005 strain treated soil was confirmed to be 80.6% (Table 2 and Fig. 3).

Changes in area of occurrence of potato scab by BIG10005 strain Pseudomonas corinisis
Whether BIG10005 strain is processed Area of disease occurrence (%) after 86 days of treatment Significant difference
(DMRT) The control
(%) I repeat Ⅱ repeat Ⅲ repeat Average process 3.5 17.6 23.2 14.8 a 80.6 No treatment 76.5 69.4 83.0 76.3 b -

Example  4. Pseudomonas Corinisis BIG10005  Analysis of Soil Microbial Density Change by Strain Treatment

The microbial density per gram of potato cultivated soil following the treatment with Pseudomonas corynsis BIG1005 strain was confirmed. Bacterial density of Pseudomonas sp. Expressing fluorescence using Kings B agar medium and Nutrient agar was used to determine the density of common bacteria using PDA medium with yeast and fungal density and starch casein agar (SCA, starch casein agar), respectively. The results of the Kings B agar medium indicate that Pseudomonas corynsis Pseudomonas fluorescence, which shows fluorescence in the soil before and after 30 days and 60 days after treatment of the BIG10005 strain, It was confirmed that the density of the inner bacteria did not decrease.

Changes in Microbial Density in Soil by Treatment with BIG10005 (log cfu / g Soil) Test medium Before strain treatment After 30 days of treatment After 45 days of treatment After 60 days of treatment King's B agar 3.56 6.2 6.13 6.5 Nutrient agar 8.9 9.5 9.62 9.01 PDA 4.5 4.3 3.98 4.1 SCA 7.5 6.4 6.01 6.13

Korea Biotechnology Research Institute KCTC12479BP 20130830

Claims (5)

Pseudomonas sp. Having potency against potato scab disease koreensis strain BIG10005 (KCTC 12479BP). A microorganism preparation for controlling potato scab disease comprising the strain of claim 1 or a culture thereof as an active ingredient. The microorganism preparation for controlling potato scabbard according to claim 2, wherein the pathogen of the potato scab disease is Streptomyces scabies . A method for controlling potato scab disease comprising the step of treating a plant or soil with the microbial agent of claim 2 or 3. A method for producing a microorganism preparation for controlling potato scab disease comprising the step of culturing the strain of claim 1.

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