KR102105252B1 - Composition for controlling Red mite using Beauveria bassiana JEF-410 and method for preventing Red mite using the same - Google Patents

Abstract

The present invention relates to a Beauveria bassiana JEF-410 strain having a chicken mite control effect, a composition for controlling chicken mite using the same, and a method for controlling chicken mite using the same.

Description

Boberia bassiana JEF-410 strain having chicken mite control effect, composition for controlling mite using chicken and method for controlling chicken mite {Composition for controlling Red mite using Beauveria bassiana JEF-410 and method for preventing Red mite using the same}

The present invention relates to a Boberia bassiana JEF-410 strain having a chicken mite control effect, a composition for controlling chicken mites using the same, and a method for controlling chicken mites using the same.

Chicken mite (wagumo, red mite) is a major cause of decline in productivity of spawning farms from spring to autumn, and is a factor that spreads major diseases and has a great influence on poultry industry. A survey of 11 countries in Europe found that 83% of all farm households were infected with chicken mites, which is estimated to amount to about $ 198 billion annually. In Korea, the infection rate of chicken mite is estimated to be over 90%, and the annual loss is known to be about 1,050 billion won.

It is reported that poultry infected with chicken mite is stressed by vampire, which causes anemia, weakness, and dropping of feathers, resulting in a decrease of about 20% in spawning and an increase in mortality of up to 4%. These chicken mites are known to be very difficult to control because they have excellent viability and can reproduce all year round, and can survive for 9 months without feeding activity, and the population doubles in 6 days at 25 ℃. .

Currently, the number of pesticides registered for the control of chicken mites in Korea is known to be 13, but five of them have been discontinued, and among the remaining four, pesticides have limitations in application that should only be used in empty houses. In reality, the number of chemicals for controlling chicken mites that can be used while chickens are kept is recognized as four types, but as the resistance of chicken mites to chemicals, residual toxicity and environmental pollution have recently increased, new organisms are emerging. The development of enemy pesticides is strongly demanded socially.

In the related art, a chicken mite pesticidal composition using an insecticidal essential oil or a food additive in connection with the control of a chicken mite has been proposed by Korean Patent Publication No. 10-2013-0142588. However, the plant extract has a limitation in that it has a control effect only on chicken mites that are in direct contact with the plant extract due to its action characteristics, and has no control effect on chicken mites that are not directly contacted, such as hiding in a barn. Until recently, there is no known technique using insect-pathogenic fungi that can effectively control even chicken mites that do not directly contact pesticides.

Accordingly, the present inventors have attempted to discover a new insect pathogenic fungus based on microorganisms and have an effective chicken mite management system, which has a control effect even when it is indirectly contacted with chicken mite, while dramatically overcoming the resistance problem and environmental residual problem of existing synthetic pesticides. I tried to be polite.

As a result, the present invention was completed by confirming the excellent insecticidal power of the Beauveria bassiana JEF-410 strain, which is harmless to humans and environmentally safe.

The present invention has been devised to solve the above problems, and the inventors have completed the present invention by confirming the excellent insecticidal power of the Beauveria bassiana JEF-410 strain.

An object of the present invention is to provide a strain of chicken mite (Beauveria bassiana) JEF-410 strain (KFCC11791P) having a control effect.

Another object of the present invention is to provide a composition for controlling chicken mites comprising a Boberia bassiana JEF-410 strain (KFCC11791P), a spore of the strain, or a culture medium of the strain.

Another object of the present invention is to provide a method for controlling chicken mites by treating the composition for controlling chicken mites.

Another object of the present invention is the Boberia bassiana (Beauveria bassiana) JEF-410 strain (KFCC11791P) and Isaria fumosorosea (Isaira fumosorosea) JEF150 (KFCC11757P), spores of the strain or chickens comprising a culture of the strain It is to provide a composition for controlling ticks.

In order to achieve the above object, the present invention can provide a Boberia bassiana (Beauveria bassiana) JEF-410 strain having a control effect of chicken mites.

According to a preferred embodiment of the present invention, the chicken mite may be Dermanyssus gallinae.

The present invention can also provide a composition for controlling chicken mites comprising a Beveria bassiana JEF-410 strain, a spore of the strain, or a culture medium of the strain.

According to a preferred embodiment of the present invention, the composition for controlling chicken mites may be formulated as any one selected from the group consisting of liquid formulations, powder formulations, and granular formulations.

According to a preferred embodiment of the present invention, the composition for controlling chicken mites may be formulated to further include any one or more selected from the group consisting of bulking agents and surfactants.

According to a preferred embodiment of the present invention, the surfactant is sodium lignosulfonate (Sodiumlignosulfonate), isotridecyl alcohol ethoxylate (isotridecyl alcohol ethoxylate) and acetylene diol (when the composition for controlling chicken mites is in liquid form) acetylene diol) may be one or more nonionic surfactants selected from the group consisting of.

According to one preferred embodiment of the present invention, the surfactant is sodium lignosulfonate or E0 / P0 / E0 block polymer (EO / PO / EO) when the composition for controlling chicken mites is in powder form or granular form. block copolymer).

According to a preferred embodiment of the present invention, the extender may be a mineral powder.

The present invention can also provide a method for controlling chicken mite comprising the step of treating the composition for controlling the aforementioned chicken mite.

The present invention also includes the control of chicken mites, including the Boberia bassiana JEF-410 strain (KFCC11791P) and Isaira fumosorosea JEF150 (KFCC11757P), spores of the strain, or cultures of the strain. It is possible to provide a composition.

When spraying chicken mite using the method for controlling chicken mite using the Boberia bassiana JEF-410 strain of the present invention, there is a significantly higher control effect than the previously reported insecticidal composition. The composition for controlling chicken mites of the present invention includes a Boberia bassiana JEF-410 strain in a spore form, and thus has a control effect against chicken mites that do not directly contact the composition. In addition, when treating with a chicken mite using the Boberia bassiana JEF-410 strain and Isaira fumosorosea JEF150 (KFCC11757P) of the present invention, when treating with each other than when treating each It has a remarkably high control effect.

Figure 1 shows the growth of the mycelium and spores observed with the naked eye (FIG. 1 (b)) observed by the naked eye (FIG. 1 (a)) and the Boberia bassiana ( Beauveria bassiana ) JEF-410 strain. .
Figure 2 shows the nucleotide sequence of the ITS (Internal Transribed Spacer) of the Beauveria bassiana ( Beauveria bassiana ) JEF-410 strain.
3 is a result of comparing and analyzing the relationship between the previously reported strains and the Boberia bassiana (Beauveria bassiana) JEF-410 strain.
4 shows spore production (FIG. 4 (a)) and solid culture production process (FIG. 4 (b)) of the Boeveria bassiana JEF-410 strain using an Italian millet.
FIG. 5 shows the insecticidal rate (short repetition) of chicken mites (FIG. 5 (a)) and the Boberia bassiana JEF-410 strain by separating treatment of the Beauveria bassiana JEF-410 strain. The chicken tick (day 14) killed by (Fig. 5 (b)) is shown.
FIG. 6 shows the insecticidal rate of chicken mites (3 repetitions) (FIG. 6 (a)) and the Boberia bassiana JEF-410 strain by separation treatment of the Boberia bassiana JEF-410 strain. Chicken mites killed by day (Day 14) (Fig. 5 (b)).
Figure 7 is a chicken mite by mixing treatment with the Beauveria bassiana JEF-410 strain and Lecanicillium attenuatum JEF 145 or Isaira fumosorosea (KFCC11757P) strain. The insecticidal rate of (Fig. 7) is shown.
Figure 8 is a schematic diagram of a method for preparing a hydrating agent using a Beveria bassiana JEF-410 strain, and a Beveria bassiana JEF-410 strain (Fig. 8 (a)) solid-cultured in millet, harvested Incorporating a mixture of spores of the Beauveria bassiana JEF-410 strain (FIG. 8 (b)) and the spores and extenders of the harvested Boberia bassiana JEF-410 strain, surfactants, and surfactants Preparation (Fig. 8 (c)).
Figure 9 is a schematic diagram of a method for preparing a liquid formulation using a Boberia bassiana JEF-410 strain, a Boberia bassiana JEF-410 strain solid cultured in millet (Fig. 9 (b)) , Spores of harvested Boberia bassiana JEF-410 strain (Fig. 9 (b)), mixture of spores and surfactants of Boberia bassiana JEF-410 strain (Fig. 9 (c) ) And Boberia bassiana (Beauveria bassiana) shows the preparation of the liquid formulation of the JEF-410 strain (Fig. 9 (d)).

Hereinafter, the present invention will be described in more detail.

As described above, it is possible to dramatically overcome the resistance problem and environmental residual problem of the existing synthetic pesticides, and improve the point that the control effect appears only when the insecticidal component based on the existing plant extract directly contacts chicken mites. There is a need to discover new insecticidal fungi based and to develop an effective chicken mite management system.

The composition for controlling chicken mite using the Beauveria bassiana JEF-410 strain according to the present invention is harmless to livestock, is safe to the environment, and provides excellent insecticidal power, and thus is effective as a composition for controlling chicken mite.

Accordingly, the present invention provides a strain of Boberia bassiana JEF-410 having a control effect against chicken mites.

In a specific embodiment of the present invention, the inventors collected soil from Jeongseon-gun, Gangwon-do. The collected soil was treated with brown larvae. As a result, brown larvae killed by insect-pathogenic fungi in the soil were obtained. A single colony of insect pathogenic fungi was isolated from the lethal brown larvae (1/4 Sabouraud dextrose agar, 25 ± 2 ℃)

In another specific embodiment of the present invention, the present inventors sequenced the strain obtained in Example 1 and finally identified as the Boberia bassiana JEF-410 strain (FIG. 2), and 15 previously reported strains. By comparing the flexible relationship between the strains of the genus Boberia (Internal transcribed spacer) and the ITS sequence (FIG. 3), the new strains of the Beauveria bassiana JEF-410 strain have genetic differences from existing strains Was confirmed.

The chicken mite is preferably but not limited to Dermanyssus gallinae, which is called Red Mite.

The present invention provides a composition for the control of chicken mites comprising a Beveria bassiana JEF-410 strain, a spore of the strain or a culture medium of the strain.

The composition for controlling the chicken mite is preferably prepared through solid culture by inoculating the suspension of the strain in the grain medium, more preferably millet, rice, Italian millet, barley, soybean , It is to use a medium consisting of one or more grains selected from the group consisting of sorghum, crude, rice, and brown rice, and among them, it is most preferable to inoculate the millet medium and solid culture (FIG. 4).

In the composition for controlling chicken mites of the present invention, the solid culture is inoculated with a suspension of the strain in a medium, and preferably cultured at 20 to 30 ° C. for 5 to 20 days, and then at room temperature. It can be dried for 30 days, more preferably after 14 days of incubation at 25 ° C, and preferably for 1 day at room temperature.

Since the composition for controlling chicken mites of the present invention contains spores of the Boberia bassiana JEF-410 strain, the spores attach to the host insects and germinate to cause fungi and the like, resulting in a pesticidal effect. In addition, in addition to chicken mites directly contacted therewith, even chicken mites not directly in contact with the composition may exhibit a control effect due to the transmission of spores.

The composition for controlling chicken mites of the present invention is preferably in the form of a liquid, powder or granule. The powder formulation means a powder form, and the granular formulation means a grain or granule form in which a solid culture is diluted 1 to 100 times by weight using an extender, but is not limited thereto.

The powder or granule formulation of the present invention is prepared by mixing a composition for controlling chicken mites, a surfactant and an extender in a weight ratio of 001 to 50%, 001 to 30% and 1 to 70%, respectively, and more preferably It is preferable to prepare by mixing at a weight ratio of 5: 5: 90, respectively (FIG. 8, Example 7). If the content of the surfactant is higher than the above ratio, economical efficiency is lowered and the control composition flows out from the chicken mite, so that a suitable control effect cannot be seen compared to the control cost, and if it is lower than the ratio, the control composition for the chicken mite is sufficient. Because it is not attached, the control effect may be deteriorated.

The liquid formulation of the present invention is prepared by mixing a composition for controlling chicken mites and a surfactant at a weight ratio of 0.01 to 80% and 0.01 to 20%, respectively, and diluting it with water at a weight ratio of 1: 10 to 1: 2000, In this case, the extender may be added at 1 to 95% by weight, preferably 1 to 50% by weight. More preferably, the composition and the surfactant for controlling chicken mite are mixed in a weight ratio of 100: 1, more preferably 2: 1, and water is 1:10 to 1: 1000, more preferably 1: 100 to water. 1: It is preferable to prepare by diluting at a weight ratio of 1000 (FIG. 9, Example 7) If the content of the surfactant is higher than the above ratio, economical efficiency is lowered and the composition for control flows out from chicken mites, which is suitable for the control cost. If the control effect is not seen, and if it is lower than the above ratio, the control effect may be lowered because the control composition is not sufficiently attached to the chicken mite.

The surfactant of the present invention means all surfactants that can be used in crop protection agents, and among them, it is preferable that they are nonionic surfactants. The nonionic surfactant is polycarboxylate, sodium lignosulfonate, sodium dialkyl sulfosuccinate, sodium alkyl sulfonate, polyoxyethylene Alkyl phenyl ether, sodium tripolyphosphate, sodium alkyl aryl sulfonate, isotridecyl alcohol ethoxylate, acetylene diol, polyoxyethylene alkyl phenyl Ether (polyoxyethylene alkyl phenyl ether), polyoxyethylene alkyl aryl phosphoric ester, polyoxyethylene alkyl aryl ether, polyoxyethylene alkyl aryl polymer, polyoxyethylene alkyl aryl polymer, poly Oxyethylene alkyl aryl polymer special, polyoxyalkylone alkyl peg Polyoxyalkylone alkyl phenyl ether, polyoxyethylene nonyl phenyl ether, sodium sulfonatenaphthalene formaldehyde, E0 / P0 / E0 block polymer (EO / PO / EO block copolymer) , Triton 100 and twin 80 are preferred.

More preferably, when the composition for controlling chicken mites is a liquid formulation, the surfactants are sodium lignosulfonate, isotridecyl alcohol ethoxylate, and acetylene diol. Any one or more selected from the group consisting of, when the composition for controlling chicken mites is a powder or granular formulation, the surfactant is sodium lignosulfonate and E0 / P0 / E0 block polymer (EO / PO / EO block) copolymer) is preferably at least one selected from the group consisting of, but is not limited thereto.

The extender of the present invention is preferably a mineral powder such as diatomaceous earth, talc, lime, apatite, dolomite, mica, kaolinite, bentonite or pyrophyllite, Pyrophyllite is most preferable, but is not limited thereto.

The present invention, Boberia bassiana (Beauveria bassiana) JEF-410 strain causes a fungus on the adult chicken mite, showing an excellent effect on the control of chicken mites.

The present invention can also provide a method for controlling chicken mite comprising the step of treating the composition for controlling the aforementioned chicken mite.

The composition for controlling chicken mites may be prepared as a suspension.

The composition for controlling chicken mites may be contact-treated or spray-treated, and most preferably spray-treated.

The suspension of the present invention is preferably prepared by harvesting spores of a cultured strain that is a solid culture or composition for controlling chicken mites, counting it with a hemocytometer, and diluting it according to the treatment concentration using various surfactants. .

In this case, when the spores are counted by a hemocytometer after solid culture is completed, the number of spores may be 1x10 ⁷ spores / g to 1x10 ¹⁰ spores (conidia) / g, preferably 5x10 ⁸ spores (conidia) It is preferred that / g to 5x10 ⁹ spores (conidia) / g.

The above-mentioned preferred types of surfactants are replaced by the above description. The treatment concentration of the suspension is preferably 1x10 ⁵ spores (conidia) to 1x10 ⁸ spores (conidia) / ml, more preferably 1 * 10 ⁷ spores (conidia) / ml.

In the method for controlling the chicken mite of the present invention, spraying the chicken mite means spraying 0.1 to 10 ml of suspension per 100 birds on the chicken mite, preferably spraying with 1 ml of suspension. . When the suspension is processed in excess of the above capacity, it is difficult to secure the economics of the control against the throughput, and when treating the suspension below the above capacity, it is difficult to see a high control activity.

When the spore suspension of the Boberia bassiana JEF-410 strain of the present invention was prepared and sprayed on a chicken mite nymph, it was confirmed that it shows high insecticidal activity (FIG. 5).

The present invention is also for the control of chicken mites comprising the Boberia bassiana JEF-410 strain (KFCC11791P) and Isaira fumosorosea JEF150 (KFCC11757P), spores of the strain, or cultures of the strain. Compositions can be provided.

When the Boberia bassiana JEF-410 strain (KFCC11791P) and Isaira fumosorosea JEF150 (KFCC11757P) strains of the present invention were sprayed with a spore suspension, chickens were treated rather than alone. It was confirmed that the insecticidal effect of ticks was improved (Fig. 6).

The chicken mite is preferably but not limited to Dermanyssus gallinae, which is called Red Mite.

The composition for controlling the chicken mite is preferably prepared through solid culture by inoculating the suspension of the strain in the grain medium, more preferably millet, rice, Italian millet, barley, soybean , It is to use a medium consisting of one or more grains selected from the group consisting of sorghum, crude, rice and brown rice, and among them, it is most preferable to inoculate the millet medium and solid culture (FIG. 6).

In the composition for controlling chicken mites of the present invention, the solid culture is inoculated with a suspension of the strain in a medium, and preferably cultured at 20 to 30 ° C. for 5 to 20 days, and then at room temperature. It can be dried for 30 days, more preferably after 14 days of incubation at 25 ° C, and preferably for 1 day at room temperature.

The composition for controlling chicken mites of the present invention is preferably in the form of a liquid, powder or granule. The powder formulation means a powder form, and the granular formulation means a grain or granule form in which a solid culture is diluted 1 to 100 times by weight using an extender, but is not limited thereto.

The present invention can also be a ratio of Boberia bassiana JEF-410 strain (KFCC11791P) and Isaira fumosorosea JEF150 (KFCC11757P), preferably from 10: 1 to 1:10. May be 5: 1 to 1: 5, and most preferably 1: 1.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as limited by these examples.

Example 1. Isolation and identification of strains

1-1. Isolation of strain

The present inventors isolated insect-pathogenic fungi from soil samples under the following conditions. After treating 5 g of primary distilled water with 20 g of collected soil, brown larvae were spun into the soil. As a result, the mycelium of the fungus was observed in the body after the brown larvae were killed. The hyphae were isolated from the body and cultured in 1/4 SDA (Sabouraud dextrose agar) at 25 ± 2 ° C.

1-2. Growth and morphology of isolated strains

The agar block cultured with the Beauveria bassiana JEF-410 strain was transferred to Sabraud Dextrose Agar (SDA) and cultured for 2 days (25 ° C, dark), followed by visual observation and optical microscopy (400 times) ( Figure 1). White spores and methods of spore formation, typical features of the strain of the genus Beauveria, have been observed.

Example 2. DNA sequencing of the Beauveria bassiana strain of Example 1

The present invention identified the Boberia bassiana of Example 1. Specifically, PCR was performed under the following conditions. 5 minutes at 94 ° C, 60 seconds at 94 ° C, 30 seconds at 62 ° C, and 30 seconds at 72 ° C were performed 30 times, and finally, post maturation was performed at 72 ° C for 3 minutes. The sequence was performed using the β-tubulin primer of Table 1 below. PCR products were electrophoresed at 140 V for 25 minutes using 0.8% agarose gel and Eco dye. The finally obtained product was sequenced and finally identified as B. bassiana (FIG. 2). The inventors of the present invention named Boberia bassiana of Example 1 as the Boberia bassiana JEF-410 strain. The β-tubulin sequence of the Beauveria bassiana JEF-410 strain is set forth in SEQ ID NO: 3. In addition to want to make a flexible relationship with the existing known Beauveria spp, the US National Biological Information Center of Beauveria sp 15 species reported in (http://www.ncbi.nlm.nih.gov/) β-tublin The sequence was compared to the flexible relationship with Beauveria JEF-410 (Fig. 3). As a result of the analysis, it was confirmed that there is a genetic difference from the known strains.

primer direction order Sequence number ITS (internal transcribed spacer) Forward 5'-CCG TAG GTG AAC CTG CGG-3 ' One Reverse 5'-TCC TCC GCT TAT TGA TAT GC-3 ' 2

Example 3. Production of spores under solid culture conditions using millet

After adding 200 g of millet to the cultured polyvinyl bag, 0.16 ml of 50% citric acid was further treated. The microwave was treated for 5 minutes so that moisture was absorbed into the millet sufficiently. The inlet part of the polyvinyl bag was prepared using a paper cup and sterile gauze for smooth oxygen supply. The polyvinyl bag containing millet was sterilized for 15 min at 121 ° C. After cooling at room temperature, 1 ml of B. bassiana JEF-410 strain suspension (1x10 ⁷ conidia / ml) was inoculated into a polyvinyl bag. After incubation for 14 days at 25 ° C, spores of each strain dried for 1 day at room temperature were finally produced (FIG. 4).

Example 4. Control effect on chicken mite of Beauveria bassiana JEF-410 strain

A spore suspension (1x10 ⁷ conidia / ml) was prepared using B. bassiana JEF-410 cultured in millet, and then sprayed with chicken mite nymphs. On the 7th day of spraying at 25 ° C. conditions, live and dead water were investigated. As a result of the experiment, JEF-410 was able to confirm the insecticidal rate of more than 50% faster than the negative control sprayed with 0.03% silwet after 3 days after treatment, and showed an insecticidal effect of more than 95% on the 5th day of treatment. It showed a higher insecticidal effect than the drug treatment (Fig. 5). Therefore, based on the results, it was judged that the B. bassiana JEF-410 strain showed higher insecticidal activity than chicken B. bassiana strain, and it was estimated that the applicability was higher than that of the resistant chemical agent.

Example 5. Control effect on chicken mite of Beauveria bassiana JEF-410 strain

A spore suspension (1 × 10 ⁷ conidia / ml) was prepared using B. bassiana JEF-410 cultured in millet, and then sprayed onto chicken mite nymphs. On the 10th day of spraying at 25 ° C, the number of live and dead insects was investigated. As a result of the experiment, JEF-410 strain was found to be 50% faster than the negative control sprayed with 0.03% silwet on the 5th day of treatment, and showed a 100% insecticidal effect on the 10th day of treatment. This showed a higher insecticidal effect than the conventional chemical treatment (Fig. 6). Therefore, based on the results, it was judged that the B. bassiana JEF-410 strain showed higher insecticidal activity than chicken B. bassiana strain, and was more applicable than the pyrethroid-based chemical drug (alpha cypermethrin) that showed resistance. It was estimated.

Example 6.Beauveria bassiana (Beauveria bassiana) JEF-410 strain and Lecanicillium attenuatum JEF 145 or Isaria fumosorosea (Isaira fumosorosea) JEF150 (KFCC11757P) Control effect on chicken mites (spray treatment)

Spore suspensions were sprayed under two conditions using B. bassiana JEF-410, L.attenuatum JEF 145 , and I.fumosorosea JEF150 cultured in millet. First, for the treatment of a single strain, a spore suspension (1x10 ⁷ conidia / ml) was prepared, and then sprayed with chicken mite nymphs. Second, in the case of the treatment of the mixed strain, a spore suspension (2x10 ⁷ conidia / ml) was prepared and then mixed under JEF-410 + JEF-145 (1: 1) or JEF-410 + JEF-150 (1: 1) conditions. Chicken mite nymphs were sprayed to be processed to a final concentration of 1x10 ⁷ conidia / ml. After spraying at 25 ° C., the number of live and dead insects was investigated every day to proceed to the 8th day. As a result of the experiment, B. bassiana JEF-410 and Isaria fumosorosea JEF-150 were confirmed to increase the insecticidal effect from day 2 to day 6 in the mixed treatment (FIG. 6). Day 2 viability was 53.2% for JEF-410 and 81.4% for JEF-150, but 35.9% for JEF-410 + JEF-150 mixed treatment, which was significantly lower than JEF-410 single strain treatment. On the fourth day, the viability was 39.4% for JEF-410 and 67.3% for JEF-150, but 22.6% for JEF-410 + JEF-150 mixed treatment, which was lower than that of JEF-410 single strain treatment. Lastly, on the 6th day, JEF-410 showed 22.5% and JEF-150 showed 44.1%, but JEF-410 + JEF-150 mixed treatment showed 17.1%, which was lower than JEF-410 single strain treatment. . Therefore, based on the results, the B. bassiana JEF-410 strain was I.fumosorosea JEF150. When mixed with the strain, it was confirmed that the insecticidal effect is improved. However, the synergistic effect of mixing with B. bassiana JEF-140 and L. attenuatum JEF-145 was not confirmed (data not shown).

Example 7. Preparation of Beauveria bassiana JEF-410 hydrating agent and liquid formulation.

The hydrating agent was prepared through the following procedure. Spores were harvested from the solid cultures of the JEF-410 strains using millet (Fig. 8 (A), (B)). The obtained spore powder (original agent), extender (pyrophyllite) and surfactant were mixed in a weight ratio of 90: 5: 5 (Fig. 8 (C)). The filtering process was performed using a sieve to secure a particle size of 300 mesh or less. Liquid formulation was prepared through the following procedure. Spores were harvested from the solid cultures of the JEF-410 strains using millet (Fig. 9 (A), (B)). The obtained spore powder (original agent) and the surfactant (surfactant) were mixed in a ratio of about 2: 1 by weight (Fig. 9 (C)). In order to secure a fine powder particle size, a fine powder was obtained using a sieve. The obtained fine powder was diluted in water to prepare a concentrated liquid formulation (FIG. 9 (d)).

[Accession Number]

Depository name: Korea Microbial Conservation Center (domestic)

Accession number: KFCC11791P

Date of Deposit: 20180829

[Accession Number]

Depository name: Korea Microbial Conservation Center (domestic)
Accession number: KFCC11757P

Date of Deposit: 20171215

Claims (9)

delete A composition for the control of chicken mites, comprising the Beauveria bassiana JEF-410 strain (KFCC11791P) and Isaira fumosorosea JEF150 (KFCC11757P), spores of the strain or a culture of the strain.
3. The composition for controlling chicken mites according to claim 2, wherein the composition for controlling chicken mites is formulated with any one selected from the group consisting of liquid formulations, powder formulations, and granular formulations.
A composition for controlling chicken mite, further comprising at least one selected from the group consisting of a bulking agent and a surfactant in the composition for controlling chicken mite of claim 2.
The method according to claim 4, wherein the surfactant is sodium lignosulfonate (isotridecyl alcohol ethoxylate) and acetylene diol (acetylene diol) when the composition for controlling chicken mites is in liquid form. A composition for controlling chicken mites, which is a nonionic surfactant having at least one selected from the group consisting of.
The method according to claim 4, wherein the surfactant is sodium lignosulfonate (Sodium lignosulfonate) or E0 / P0 / E0 block polymer (EO / PO / EO block copolymer) when the composition for controlling chicken mites is in powder form or granular form. Non-ionic surfactant, composition for controlling chicken mites.
The composition for controlling chicken mites according to claim 4, wherein the extender is a mineral powder.
A method of controlling chicken mite by treating the composition for control of claim 3. delete

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