KR101465070B1 - Composition for improving milk protein synthesis containing Selenium, feed additive comprising the same and method for improving milk protein synthesis using the same - Google Patents

Abstract

The present invention relates to a composition for promoting the synthesis of milk protein comprising a selenium component, preferably a nanosom containing a selenium component, as an active ingredient. Accordingly, the present invention can be applied as a feed additive to increase the milk protein of the milk obtained from the livestock fed the milk, thereby improving the milk quality. In addition, the content of selenium can be increased in meat and milk of livestock to which the present invention is applied.

Description

[0001] The present invention relates to a composition for promoting milk protein synthesis including selenium, a feed composition containing the same, and a method for enhancing milk protein synthesis using the same.

The present invention relates to a composition for promoting synthesis of a milk protein, a method for producing the same, and a method for enhancing the synthesis of milk protein using the same.

Selenium functions as an enzyme that contains selenium in the human body. Typical selenium-containing enzymes include glutathione peroxidase (GSHPx), thioredoxin, selenoprotein P, or iodothyronine deiodinase. Glutathione peroxidase and thioredoxin function as an antioxidant nutrient that inhibits oxidative stress-induced cell damage by degrading hydrogen peroxide in the body. It is also known that selenium protein P is also antioxidant.

In addition, selenium is known as an essential nutrient. In the absence of selenium, Kessan disease, a kind of cardiac muscle disease, Kashin Beck disease, osteoarthritis, which occurs just before puberty or puberty, myalgia, muscle exhaustion, and cardiomyopathy occur. Various cardiac dysfunctions appear.

On the other hand, milk protein is a protein derived from milk. Milk protein has many amino acids which are insufficient in vegetable protein, such as lysine and methionine, which are essential amino acids. Although many attempts have been made to increase the content of such milk proteins in the past, there has not yet been an effective method for increasing the content of milk proteins.

The present invention provides a composition for increasing milk protein. In addition, a problem to be solved by the present invention is to provide a method for raising milk protein in an animal producing milk.

In order to solve the above-mentioned problems, the present invention provides a feed composition for enhancing milk protein synthesis comprising an effective ingredient of a nanosom containing a selenium component, preferably a selenium component.

The selenium component may be provided as selenium or a mixture of selenium and cysteine.

The nanosome may be prepared by a mixture comprising a phospholipid and water, and the phospholipid may be any one selected from the group consisting of lecithin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, and dipalmitoylphosphaticholine.

The present invention also provides a feed additive for enhancing milk protein synthesis, comprising the composition according to the present invention.

The present invention also provides a method of enhancing milk protein synthesis in an animal by administering to the animal an effective amount of a nanosom containing a selenium component, preferably a selenium component.

The present invention can enhance the synthesis of milk proteins in mammary cells through nanosomes containing a selenium component, preferably a selenium component. As a result, the milk protein content of milk obtained from livestock is increased, and milk quality can be improved. In addition, by increasing the absorption rate of selenium as nanosizing of the selenium component, it is possible to promote the physiological activity of the livestock and to increase the selenium content in the meat or milk using the livestock, thereby producing high quality meat and milk.

BRIEF DESCRIPTION OF THE DRAWINGS The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate exemplary embodiments of the invention and, together with the description of the invention, It should not be construed as limited.
FIG. 1 is a graph of the cell viability test result of the nanosome according to the present invention.
FIG. 2 is a graph showing cell viability test results of the nanosome according to the present invention. FIG.
FIG. 3 is a graph showing the results of experiments on the expression level of bCSNB in the nanosome according to the present invention.

Hereinafter, the present invention will be described in detail. The terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms and the inventor may appropriately define the concept of the term in order to best describe its invention It should be construed as meaning and concept consistent with the technical idea of the present invention. Therefore, the constitution described in the embodiments described herein is merely the most preferred embodiment, and does not represent all of the technical ideas of the present invention, so that various equivalents and variations And the like.

The present invention provides a composition for promoting the synthesis of milk protein, which comprises, as an active ingredient, a nanosom containing a selenium component, preferably a selenium component.

In the present invention, milk protein is exemplified by milk protein as milk protein, and the composition according to the present invention can enhance the synthesis of milk protein, and such composition can be applied to feed additives to be fed to livestock. The feed additive according to the present invention is a feed additive to be applied to livestock producing dairy products such as dairy cattle, goat, goat and goat. The feed additive according to the present invention enhances the milk protein content of milk obtained from livestock .

The present inventors have found that the survival ability of mammary gland cells is enhanced through the nano-som containing the selenium component, preferably the selenium component, and the expression amount of the milk protein synthesis gene is increased. The present invention has been completed based on the fact that the present invention can be used for enhancing the synthesis of milk protein using selenium according to the present invention, preferably nanosome containing selenium. That is, the present invention provides a composition comprising a nanosome containing a selenium component, preferably a selenium component, and a feed additive comprising the same, wherein the feed additive is applied to a livestock producing milk, To promote milk protein more.

In addition, the content of selenium is also increased in livestock meat and milk to which the feed additive including the composition according to the present invention is applied. Selenium is known as an essential nutrient, and is especially needed for pregnant women and breastfeeding women. The main source of selenium is meat and milk, and the composition according to the present invention more preferably, the amount of selenium is increased in meat and milk obtained from livestock to which the feed additive according to the present invention is administered, and the consumption of selenium Can increase.

The selenium component according to the present invention may be comprised of selenium or a mixture of selenium and cysteine. At this time, selenium preferably contains selenium in the form of sodium selenite.

In addition, nanosomes containing a mixture of selenium and cysteine are more preferable than nosomes containing selenium in that they have higher cell viability and do not increase cytotoxicity. More specifically, the nano-somatization of a mixture of selenium and cysteine can be synergistic through the interaction between cysteine and other nanosome components. Thus, through a feed additive comprising a nanosome containing a mixture of selenium and cysteine, it can exhibit a remarkable effect of promoting the milk protein of milk obtained from livestock to which the feed additive is administered.

When a mixture of selenium and cysteine is applied, the weight ratio of selenium and cysteine is preferably 1: 1.

The various types of selenium contained in the nanosome are preferably used in an amount of 5 to 20% by weight, more preferably 5 to 15% by weight, and more preferably 10 to 30% by weight, based on the total weight of the mixture for preparing nanosome Is more preferably used.

The nanosomes are prepared by a mixture comprising a phospholipid, and water. Or a solvent other than the water for dissolving the phospholipid may be further added according to the properties of the phospholipid. For example, all kinds of solvents capable of dissolving phospholipids such as ethanol may be added, One, ethanol is preferred.

Nanosomes containing a selenium component can be prepared by a mixture comprising phospholipids, selenium components, water, and the like. Alternatively, other solvents may be added in addition to water for dissolving the phospholipids according to the characteristics of the phospholipids. For example, all kinds of solvents capable of dissolving ethanol and the like may be added, Ethanol may be added and is not limited to this kind.

Nanosome is a substance expressed in various ways by nanosome, micelle, liposome and the like, and is not limited to this name. Is a thermodynamically stable and homogeneous structure made up of amphiphilic, for example, low molecular weight materials having both a hydrophilic group and a hydrophobic group at the same time. The amphipathic substance is an amphipathic substance in the aqueous solution, the hydrophilic group of the amphipathic substance is collected externally, and the hydrophobic group is gathered to form a layer, thereby forming a myoshell as a spherical association. These my shells can be represented as capsules and their names can vary. In addition, the layer by the phospholipid may be a bilayer or a multilayer as well as a single layer, and the number of such phospholipid layers is not limited. Mycels or capsules made with such amphiphilic phospholipids have an average particle size of nanoparticle size, for example from 1 to 1000 nm.

The selenium component may be contained in the center of the my shell or between the phospholipid layer and the layer. For example, selenium and cysteine can be naturally encapsulated as a single substance in the center of the my shell or between the phospholipid layer and the layer. In addition to increasing the uptake rate of selenium into the livestock through the nano-souring of the selenium component, the present invention can further enhance the synthesis of milk protein through the nano-somenification of the selenium component.

In the present invention, the microshell having an average particle diameter of 1 to 500 nm is preferable, and the microshell is more preferably an average particle diameter of 10 to 400 nm. It is more preferable that the average shell diameter of the shell is 100 to 300 nm. This is because, in the case of the average particle size of the my shell, not only the stability of the my shell particle but also the ability of the active substance to penetrate and the bio-absorption can be increased.

As the phospholipid contained in the nanosome, various phospholipids known in the art can be used. Examples of the phospholipid include phospholipids such as lanolin such as egg yolk lecithin, soybean lecithin and hydrogenated lecithin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, Choline, etc. may be used. The phospholipid is preferably 2 to 10% by weight based on the total weight of the mixture for preparing the nanosome.

The present invention may further comprise a polyol for stabilizing the nanosome as an emulsion stabilizing adjuvant in a mixture for making the nanosome. As the polyol, propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methylpropanediol, isoprene glycol, pentylene glycol, erythritol, xylitol, sorbitol and the like can be used. Propylene glycol, and the like are more preferable. Such a polyol is preferably used in an amount of 2 to 15% by weight, more preferably 5 to 10% by weight, based on the total weight of the mixture for preparing the nanosome containing the active ingredient.

The present invention may further comprise a surfactant for stabilizing the nanosome in the mixture for making the nanosome. As such a surfactant, any of those known in the art may be used, and for example, an anionic surfactant, a cationic surfactant, a positive surfactant or a nonionic surfactant may be used, and preferably an anionic surfactant Or a nonionic surfactant is used. Specific examples of anionic surfactants include alkyl acyl glutamates, alkyl phosphates, alkyl lactylates, dialkyl phosphates, alkyl lactates and trialkyl phosphates. Specific examples of nonionic surfactants include alkoxylated alkyl ethers, alkoxylated alkyl esters, alkyl polyglycosides, polyglyceryl esters and sugar esters. More preferably, the surfactant is diacetyl phosphate. The content of the surfactant is preferably 0.5 to 1.5% by weight, more preferably 0.8 to 1.0% by weight, based on the total weight of the water-containing mixture for preparing the nanosome containing the active ingredient.

The nanosome of the present invention can be extruded through a filter having a straight passage or a curved passage or homogenized through a homogenizer to have a reduced uniform size distribution and the prepared solution can be dewatered or lyophilized have.

The present invention also provides a method for producing a nanosome containing an active ingredient according to the present invention by passing a mixture containing a selenium component, a phospholipid and water to a high pressure homogenizer or the like.

The present invention also provides a method for enhancing milk protein using a composition comprising as an active ingredient a nanosom containing a selenium component, preferably a selenium component. That is, the present invention provides a method for increasing the content of milk protein in milk obtained by administering selenium to an animal (i.e., a livestock producing milk such as cow, goat and chlorine) in an effective amount.

Hereinafter, embodiments of the present invention will be described in detail to facilitate understanding of the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art.

≪ Preparation of nanosome containing selenium >

Example 1

Lecithin (Lipoid S from Pharmachem) and ethanol are mixed and dissolved, and then mixed with selenium-cysteine, a 1: 1 mixture of selenium and cysteine. After that, water was added and stirred until completely dissolved to prepare a mixture. The respective contents in the mixture were 10% by weight of the active ingredient, 5% by weight of lecithin, 10% by weight of ethanol and 75% by weight of water. That is, the constituent components are 5 wt% of selenium, 5 wt% of cysteine, 10 wt% of ethanol, 5 wt% of lecithin and 75 wt% of distilled water. After that, the nanotubes were processed by using the MN400 of MICRONOX Co., Ltd., and the pressure was cycled three times at 1000 bar.

<Experiments on cell viability>

The cell line used was the MAC-T cell line, a bovine-derived mammary cell.

The medium composition of MAC-T cells was DMEM medium containing 100 units / ml of penicillin, 100 ug / ml of streptomycin, 5 ug / ml of insulin, 50 ug / ml of gentamicin, 1 ug / ml of hydrocortisone and 10% of FBS.

Another cell line was MDBK, a bovine kidney cell.

The medium composition of the MDBK cells was DMEM medium containing 100 units / ml penicillin, 100 ug / ml streptomycin, 50 ug / ml gentamycin and 10% FBS.

This experiment was conducted to measure cell viability. Yellow tetrazolium salt MTT (MTT) was reduced from living cells to water-insoluble formazan crystals. The crystals were dissolved in DMSO And the amount of the generated crystal was measured by a spectroscopic method. As a result, as the number of living cells increases, the number of these crystals increases. Therefore, the viability of cells can be easily measured by this method.

First, the MAC-T cells were plated on a 12-well plate at 2 x 10 &lt; ^{4 &} gt ;. Experimental Example 1-1) Selenium 18 mg / ml, Experimental Example 1-2 Nanosomes containing selenium-cysteine 18 mg / ml were treated in each cell. MTT solution was then added to each well at a volume of 10% and placed in a 37 ° C CO ₂ incubator for 2 to 3 hours. Then, the medium was removed, and 500 μl of DMSO was added thereto. After thoroughly mixing with pipetting, absorbance was measured at 570 nm.

In addition, laying crushing the MDBK cells in place of the MAC-T cell, Experimental Example 2-1) selenium 18㎎ / ml in Experimental Example 2-2) selenium-treated nano-containing cysteine 18㎎ / ml cotton in each cell. Others proceeded in the same manner as the MAC-T cells.

The results are shown in FIGS. 1 and 2. Referring to FIG. 1, it was confirmed that MAC-T cells treated with nanosome containing selenium-cysteine had higher viability, ie, viability of mammary cells than MAC-T cells treated with non-nanosome-free selenium. 2, MDBK cells treated with nanosomes containing selenium-cysteine were found to have higher viability than MDBK cells treated with non-nanosome-free selenium. In other words, Respectively.

&Lt; Experimental Expression of mRNA Expression Related to Milk Protein Synthesis in MAC-T Cells &

Experimental Example  3-1

Example 1, which is a nanosome containing selenium-cysteine, is reacted with MAC-T cells for 24 hours. Then, RNA is extracted by chloroform extraction and cDNA is synthesized using reverse transcriptase. The reaction was carried out at 25 ° C for 10 minutes, then at 48 ° C for 120 minutes, then at 95 ° C for 5 minutes.

Then, real-time PCR is performed using Primer and SYBR corresponding to the indicator. The reaction conditions are annealing at 95 ° C for 40 cycles and at 60 ° C for 1 minute.

The primers used were as follows.

Experimental Example 3-2

The same experiment was performed except that the nanosome containing the selenium-cysteine was reacted.

 The expression level of bCSNB gene, one of the synthetic genes of β-casein in milk protein, is shown in FIG. FIG. 3 shows that the nanosome containing selenium-cysteine causes more genes to express milk protein.

Claims (11)

A composition for promoting the synthesis of a milk protein, which comprises, as an active ingredient, a nanosome containing a mixture component of selenium (Selenium) and cysteine. delete delete The method according to claim 1,
Wherein the nanosome is produced by a mixture comprising a phospholipid and water. 5. The method of claim 4,
Wherein the phospholipid is at least one selected from the group consisting of lecithin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, and dipalmitoylphosphaticholine. A feed additive for milk protein promotion comprising a composition according to any one of claims 1 and 5 to 5. A method of enhancing milk protein synthesis in an animal by administering to the animal an effective amount of a nanosome containing a mixture of selenium and cysteine. delete delete 8. The method of claim 7,
Wherein said nanosome is produced by a mixture comprising a phospholipid and water. 11. The method of claim 10,
Wherein the phospholipid is at least one selected from the group consisting of lecithin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, and dipalmitoylphosphaticholine.

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