KR101533638B1 - Composition for improving milk protein synthesis containing Selenium and Methionine, feed additive comprising the same and method for improving milk protein synthesis using the same - Google Patents

Abstract

The present invention relates to a composition for enhancing the synthesis of milk protein comprising selenium and a methionine component, preferably a nanosome containing selenium and a methionine component as an active ingredient. Accordingly, the present invention can be applied as a feed additive to increase the milk protein of the milk obtained from the livestock fed the milk, thereby improving the milk quality. In addition, the content of selenium can be increased in meat and milk of livestock to which the present invention is applied.

Description

TECHNICAL FIELD The present invention relates to a composition for promoting milk protein synthesis including selenium and methionine, a feed composition containing the same, and a method for enhancing milk protein synthesis using the same. using the same}

The present invention relates to a composition for promoting synthesis of a milk protein, a method for producing the same, and a method for enhancing the synthesis of milk protein using the same.

Selenium functions as an enzyme that contains selenium in the human body. Typical selenium-containing enzymes include glutathione peroxidase (GSHPx), thioredoxin, selenoprotein P, or iodothyronine deiodinase. Glutathione peroxidase and thioredoxin function as antioxidant nutrients that inhibit cellular damage caused by oxidative stress by decomposing hydrogen peroxide in the body, and selenium protein P is also known to have antioxidant functions.

In addition, selenium is known as an essential nutrient. In the absence of selenium, Kessan disease, a kind of cardiac muscle disease, Kashin Beck disease, osteoarthritis, which occurs just before puberty or puberty, myalgia, muscle exhaustion, and cardiomyopathy occur. Various cardiac dysfunctions appear.

On the other hand, milk protein is a protein derived from milk. Milk protein has many amino acids which are insufficient in vegetable protein, such as lysine and methionine, which are essential amino acids. Although many attempts have been made to increase the content of such milk proteins in the past, there has not yet been an effective method for increasing the content of milk proteins.

Korean Patent Publication No. 10-2005-0109637

The present invention provides a composition for increasing milk protein. In addition, a problem to be solved by the present invention is to provide a method for raising milk protein in an animal producing milk.

In order to solve the above problems, the present invention provides a dietary composition for enhancing the synthesis of milk protein comprising an active ingredient of selenium and a methionine component, preferably a nanosome containing selenium and a methionine component do.

According to a preferred embodiment of the present invention, the weight ratio of the selenium and methionine components may be about 1: 1.

According to another preferred embodiment of the present invention, the nanosome can be prepared by a mixture comprising a phospholipid and water, wherein the phospholipid is selected from the group consisting of lecithin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and dipalmitoylphosphatocholic And the like.

According to another aspect of the present invention, there is provided a feed additive for enhancing milk protein synthesis, comprising a composition according to the present invention.

According to another aspect of the present invention, the present invention provides a method of treating cancer in an animal by administering to the animal an effective amount of nanosom containing selenium and methionine components, preferably selenium and methionine components, Lt; RTI ID = 0.0 > synthesis. ≪ / RTI >

The present invention can enhance the synthesis of milk proteins in mammary gland cells through nano-somes containing selenium and methionine components, preferably selenium and methionine components. As a result, the milk protein content of milk obtained from livestock is increased, and milk quality can be improved. In addition, by increasing the absorption rate of selenium as a nano-sizing of selenium and methionine components, it is possible to promote the physiological activity of the livestock fed the selenium and increase the selenium content in meat or milk using livestock, thereby producing high quality meat and milk.

BRIEF DESCRIPTION OF THE DRAWINGS The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate exemplary embodiments of the invention and, together with the description of the invention, It should not be construed as limited.
1 is a graph showing the cell viability test result of the composition according to the present invention.
Fig. 2 is a graph showing the test results of the expression amount of bCSNAS gene on the composition of the present invention.
FIG. 3 is a graph showing the results of experiments on the expression level of bCSNK in the nanosome according to the present invention.

Hereinafter, the present invention will be described in detail. The terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms and the inventor may appropriately define the concept of the term in order to best describe its invention It should be construed as meaning and concept consistent with the technical idea of the present invention. Therefore, the constitution described in the embodiments described herein is merely the most preferred embodiment, and does not represent all of the technical ideas of the present invention, so that various equivalents and variations And the like.

The present invention provides a composition for enhancing milk protein synthesis comprising as an active ingredient a nanosom containing selenium and a methionine component, preferably selenium and a methionine component.

In the present invention, milk protein is exemplified by milk protein as milk protein, and the composition according to the present invention can enhance the synthesis of milk protein, and such composition can be applied to feed additives to be fed to livestock. The feed additive according to the present invention is a feed additive to be applied to livestock producing dairy products such as dairy cattle, goat, goat and goat. The feed additive according to the present invention enhances the milk protein content of milk obtained from livestock .

The present inventors have found that the expression amount of a milk protein synthesis gene is increased through a nano-som containing selenium and a methionine component, preferably a selenium and a methionine component, and thus the above-mentioned active ingredient is used for the purpose of promoting the synthesis of milk protein And thus the present invention has been completed. That is, the present invention provides a composition comprising a nano-som containing selenium and a methionine component, preferably a selenium and a methionine component, and a feed additive comprising the same, wherein the feed additive is applied to a livestock producing milk, To increase milk protein in milk.

In addition, the content of selenium is also increased in livestock meat and milk to which the feed additive including the composition according to the present invention is applied. Selenium is known as an essential nutrient, and is especially needed for pregnant women and breastfeeding women. The main source of such selenium is meat and milk, and the composition according to the present invention more preferably the selenium content in meat and milk obtained from the livestock to which the feed additive according to the present invention is administered is increased and the consumption of selenium .

The active ingredient of the composition for enhancing the synthesis of a milk protein according to the present invention is characterized in that it is a mixture of selenium and methionine, that is, selenium and methionine are added at the same time, and when two components are used together, the remarkable effect of the present invention can be obtained. At this time, selenium may include selenium in the form of sodium selenite.

In addition, it is expected that the inclusion of selenium and methionine as an active ingredient may have the effect of a milk protein synthesis which is more favorable than combinations with other amino acid components.

In addition, all of the nano-somatosensory mixtures of selenium and methionine are nano-encapsulated, but the nano-encapsulated mixture of selenium and methionine is expressed in the gene of bCSNAS, one of the synthetic genes of α-Casein And the expression level of bCSNK gene, which is one of the synthetic genes of κ-Cacein, is remarkably increased. In particular, it is more preferable that the active ingredient is nano-encapsulated. In addition, even when the active ingredient is treated with nano-encapsulated cells, it can have a cell viability similar to that obtained when the nano-encapsulated cells are treated.

The selenium and methionine components contained in the nanosome are preferably used in an amount of 5 to 20% by weight, more preferably 5 to 15% by weight, and more preferably 10 to 30% by weight, based on the total weight of the mixture for preparing nanosome Is more preferably used.

The nanosomes are prepared by a mixture comprising a phospholipid, and water. Or a solvent other than the water for dissolving the phospholipid may be further added according to the properties of the phospholipid. For example, all kinds of solvents capable of dissolving phospholipids such as ethanol may be added, One, ethanol is preferred.

Nanosomes containing selenium and methionine components can be prepared by a mixture comprising phospholipids, selenium and methionine components, water, and the like. Or a solvent other than water for dissolving the phospholipid may be further added according to the properties of the phospholipid. For example, any kind of solvent capable of dissolving phospholipids such as ethanol may be added, preferably ethanol And it is not limited to this kind.

Nanosome is a substance expressed in various ways by nanosome, micelle, liposome and the like, and is not limited to this name. Is a thermodynamically stable and homogeneous structure made up of amphiphilic, for example, low molecular weight materials having both a hydrophilic group and a hydrophobic group at the same time. The amphipathic substance is an amphipathic substance in the aqueous solution, the hydrophilic group of the amphipathic substance is collected externally, and the hydrophobic group is gathered to form a layer, thereby forming a myoshell as a spherical association. These my shells can be represented as capsules and their names can vary. In addition, the layer by the phospholipid may be a bilayer or a multilayer as well as a single layer, and the number of such phospholipid layers is not limited. Mycels or capsules made with such amphiphilic phospholipids have an average particle size of nanoparticle size, for example from 1 to 1000 nm.

The selenium and methionine components may be contained in the center of the my shell or between the phospholipid layer and the layer. For example, selenium and methionine components can be naturally encapsulated as a single substance in the center of the my shell or between the phospholipid layer and the layer. In addition to enhancing the uptake rate of selenium into the livestock through nano-souring of the selenium and methionine components, the present invention can further enhance the synthesis of milk protein through the nano-somatization of the selenium component.

In the present invention, the microshell having an average particle diameter of 1 to 500 nm is preferable, and the microshell is more preferably an average particle diameter of 10 to 400 nm. It is further preferable that the average shell diameter of the shell is 100 to 300 nm. This is because, in the case of the average particle size of the my shell, not only the stability of the my shell particle but also the ability of the active substance to penetrate and the bio-absorption can be increased.

As the phospholipid contained in the nanosome, various phospholipids known in the art can be used. Examples of the phospholipid include phospholipids such as lanolin such as egg yolk lecithin, soybean lecithin and hydrogenated lecithin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, Choline, etc. may be used. The phospholipid is preferably 2 to 10% by weight based on the total weight of the mixture for preparing the nanosome.

The present invention may further comprise a polyol for stabilizing the nanosome as an emulsion stabilizing adjuvant in a mixture for making the nanosome. As the polyol, propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methylpropanediol, isoprene glycol, pentylene glycol, erythritol, xylitol, sorbitol and the like can be used. Propylene glycol, and the like are more preferable. Such a polyol is preferably used in an amount of 2 to 15% by weight, more preferably 5 to 10% by weight, based on the total weight of the mixture for preparing the nanosome containing the active ingredient.

The present invention may further comprise a surfactant for stabilizing the nanosome in the mixture for making the nanosome. As such a surfactant, any of those known in the art may be used, and for example, an anionic surfactant, a cationic surfactant, a positive surfactant or a nonionic surfactant may be used, and preferably an anionic surfactant Or a nonionic surfactant is used. Specific examples of anionic surfactants include alkyl acyl glutamates, alkyl phosphates, alkyl lactylates, dialkyl phosphates, alkyl lactates and trialkyl phosphates. Specific examples of nonionic surfactants include alkoxylated alkyl ethers, alkoxylated alkyl esters, alkyl polyglycosides, polyglyceryl esters and sugar esters. More preferably, the surfactant is diacetyl phosphate. The content of the surfactant is preferably 0.5 to 1.5% by weight, more preferably 0.8 to 1.0% by weight, based on the total weight of the water-containing mixture for preparing the nanosome containing the active ingredient.

The nanosome of the present invention can be extruded through a filter having a straight passage or a curved passage or homogenized through a homogenizer to have a reduced uniform size distribution and the prepared solution can be dewatered or lyophilized have.

The present invention also provides a method for producing a nanosome containing an active ingredient according to the present invention by passing a mixture containing selenium and a methionine component, a phospholipid and water to a high pressure homogenizer or the like.

The present invention also provides a method for enhancing milk protein using a composition comprising as an active ingredient nanosomes containing selenium and a methionine component, preferably selenium and a methionine component. That is, the present invention provides a method for increasing the content of milk protein in milk obtained by administering an effective amount of selenium and methionine components to an animal (i.e., a livestock producing milk such as cows, goats and chlorine).

Hereinafter, embodiments of the present invention will be described in detail to facilitate understanding of the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art.

<Examples>

Example 1 (hereinafter referred to as selenium-methionine, Selenium-Methionine or SM)

A selenium-methionine component, a 1: 1 mixture of selenium and methionine, was prepared.

Example 2 (hereinafter referred to as a selenium-methionine nanosome, Selenium-Methionine Nanosome or SMN)

Lecithin (Lipoid S from Pharmachem) and ethanol are mixed and dissolved, and then selenium-methionine, a 1: 1 mixture of selenium and methionine, is mixed. After that, water was added and stirred until completely dissolved to prepare a mixture. The respective contents in the mixture were 10% by weight of the active ingredient, 5% by weight of lecithin, 10% by weight of ethanol and 75% by weight of water. That is, the components are composed of 5% by weight of selenium, 5% by weight of methionine, 10% by weight of ethanol, 5% by weight of lecithin and 75% by weight of distilled water. After that, the nanotubes were processed by using the MN400 of MICRONOX Co., Ltd., and the pressure was cycled three times at 1000 bar.

<Experiments on cell viability>

The cell line used was the MAC-T cell line, a bovine-derived mammary cell. The medium composition of the MAC-T cells was DMEM medium containing 100 units / ml of penicillin, 100 ug / ml of streptomycin, 5 ug / ml of insulin, 50 ug / ml of gentamicin, 1 ug / ml of hydrocortisone and 10% of FBS. This experiment was conducted to measure cell viability. Yellow tetrazolium salt MTT (MTT) was reduced from living cells to water-insoluble formazan crystals. The crystals were dissolved in DMSO And the amount of the generated crystal was measured by a spectroscopic method. As a result, as the number of living cells increases, the number of these crystals increases. Therefore, the viability of cells can be easily measured by this method.

First, the MAC-T cells were plated on a 12-well plate at 2 x 10 &lt; ^{4 &} gt ;. Example 1 (SM) and Example 2 (SMN) were treated with 18 占 퐂 / ml to 300 占 퐂 / ml for each cell. MTT solution was then added to each well at a volume of 10% and placed in a 37 ° C CO ₂ incubator for 2 to 3 hours. Then, the medium was removed, and 500 μl of DMSO was added thereto. After thoroughly mixing with pipetting, absorbance was measured at 570 nm.

The results of the above cell viability are shown in Fig. Referring to FIG. 1, it was confirmed that the cell viability was more than 80% in both Example 1 in which nano-shedding was not performed and Example 2 in which nano-shedding was performed.

&Lt; Experimental Expression of mRNA Expression Related to Milk Protein Synthesis in MAC-T Cells &

The expression levels of bCSNAS gene and one of the synthetic genes of κ-Casein in one of the synthetic genes of α-Casein were determined by treating MAC-T cells with 100 μg / ml of Example 1 (SM) and Example 2 (SMN) The expression level of one bCSNK gene was confirmed

More specifically, the components of Example 1 and Example 2 were reacted for 24 hours, RNA was extracted by chloroform extraction, and cDNA was synthesized using reverse transcriptase. The reaction was carried out at 25 ° C for 10 minutes, then at 48 ° C for 120 minutes, then at 95 ° C for 5 minutes.

Then, real-time PCR is performed using Primer and SYBR corresponding to the indicator. The reaction conditions are annealing at 95 ° C for 40 cycles and at 60 ° C for 1 minute.

The primers used were as follows.

The expression level of bCSNAS, which is one of the synthetic genes of α-casein in milk protein, is shown in FIG. 2. The expression level of bCSNK, which is one of the synthetic genes of κ-casein in milk protein, Respectively. Referring to FIG. 2, both the nano-cocooned Example 2 and the non-nano-cocooned Example 1 exhibited a high expression level for the bCSNAS gene, and the nano-cocooned Example 2 was increased by about 40%. In addition, as shown in FIG. 3, both the nano-encapsulated Example 2 and the non-nano-encapsulated Example 1 exhibited a high expression level on the bCSNK gene, and in particular, the nano-encapsulated Example 2 was increased by about 52% have.

Claims (11)

A composition for enhancing a milk protein synthesis, which comprises selenium and methionine as an active ingredient. The method according to claim 1,
Wherein said composition comprises nanosomes containing selenium and a methionine component as an effective ingredient. The method according to claim 1,
Wherein the weight ratio of the selenium and methionine components is 1: 1. 3. The method of claim 2,
Wherein the nanosome is produced by a mixture comprising a phospholipid and water. 5. The method of claim 4,
Wherein the phospholipid is at least one selected from the group consisting of lecithin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, and dipalmitoylphosphaticholine. A feed additive for enhancing milk protein comprising the composition according to any one of claims 1 to 5. A method of promoting milk protein synthesis in an animal characterized by administering to the animal an effective amount of Selenium and Methionine components. 8. The method of claim 7,
A method of promoting milk protein synthesis in an animal, comprising administering to the animal an effective amount of nanosomes comprising selenium and a methionine component. 8. The method of claim 7,
Wherein the weight ratio of selenium and methionine components is 1: 1. 9. The method of claim 8,
Wherein the nanosome is produced by a mixture comprising a phospholipid and water. 11. The method of claim 10,
Wherein the phospholipid is at least one selected from the group consisting of lecithin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, and dipalmitoylphosphaticholine.

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