KR101450813B1 - Health functional food comprising extracts of herbal mixture for releasing stress - Google Patents

Abstract

The present invention relates to a health functional food, comprising extracts from a herbal mixture including Dendranthema indicum (L.) Des Moul., Prunella vulgaris, Elsholtzia ciliata, Curcuma and Mentha arvensis var. piperascens. A composition including the extracts from the herbal mixture has greater effect in relieving stress and is used as food in relieving stress for people who are exhausted such as students, office workers and others.

Description

[0001] The present invention relates to a health functional food containing extracts of herbal mixtures for releasing stress,

The present invention relates to a health functional food for relieving stress containing an extract of a crude drug mixture, and more particularly, to a health functional food for stress relief comprising an extract of a crude drug mixture containing ginger, Functional foods.

Today, modern people are suffering from chronic stress and fatigue due to overwork and busy daily life. Such chronic stress is a major cause of adult diseases such as hypertension, diabetes and cancer as well as irregular eating habits. In Korea, where culture is dominant to be solved to solve this problem, Korea tends to lead to excessive drinking particularly. And exercise and diet are used a lot. In most cases, however, there is a temporary stress effect, but there is a weakness in the fundamental solution. In recent years, a variety of drinking agents including herbal medicines for relieving stress have been commercially available, and such drinking agents tend to avoid consumers because of their unique irritants and strong taste. In addition, although it exhibits the stress relieving effect in its own way, other functions can not be expected and can not induce recovery of the fundamental body.

Particularly, most of the recent contents invented based on the record in the old classic doctrine are carried out in a part of the protection function. However, since the primary gastrointestinal and liver function disorders are accompanied by stress, when the stress is severe, the gastrointestinal function is significantly deteriorated and seriously, the function of the long-term functional role is disturbed. Therefore, there is a problem that it is difficult to solve the problem with the conventional method.

On the other hand, chronic fatigue is caused by accumulation of fatigue due to irregular eating habits and frequent drinking culture due to increased stress. Medically, mental problems such as stress, depression, and anxiety disorders account for about 50% of these fatigue symptoms.

Chrysanthemum indicum is also called 黄菊. In October, the flowers are dried and drunk, and young leaves are used as herbs. There is a strong aroma in the flower, and it is also used for ornamental purposes. It is prescribed for the treatment of hot flushes, pneumonia, bronchitis, headache, gastritis, enteritis, boils in one room. It has a natural antibiotic effect because it has a detoxifying action. In the case of folk remedies, the entire paste is applied to the affected area or the affected area is rinsed with water. It is distributed in Korea, Taiwan, China and Japan.

Prunella vulgaris is a perennial herbaceous outpost of Lamiaceae belonging to Lamiaceae. It is a medicinal herb that has many calyxes and leaves attached to its flowers, stems on its lower part and small hairs of white birds. And there is little taste. It is said to be in the summer when other plants grow vigorously, and it is called candles. It is good to be used as a medicinal product in which the shape of the flower is yellowish brown and dried without breaking. And the candle cleanses the liver (肝气) and releases the urine (acupuncture), acute mastitis, breast cancer, night-time eye pain, difficult to see the light and tears, dizziness of the head and eyes, Is known as a medicine to treat. It has a natural antibiotic effect because it has a detoxifying action.

Seaweed ( Elsholtzia ciliata ) is a herbaceous plant with early spring. It is also commonly used for the treatment of sweating, fever, diuretic, sweating as a hemostatic agent, each species, species, gastritis, hematocrit and bad breath.

In Korea, it is the root of the ginger root ( Curcuma longa ), which is removed from the roots of the ginger root, or steamed and dried. In Japan, it is root stem. In China, it refers to the roots of Curcuma kwangsiensis , Curcuma wenyujin , and Curcuma phaeocaulis , plants of the genus Ulva and Ginger. Ulgum has similar appearance to turmeric, but both sides of the leaf are smooth. It grows naturally in Southeast Asia including southern China, India, and Okinawa, and is cultivated in the southern part of Korea. When mixed with alcohol, it was named as yellow, like gold. It is called magic because its shape is similar to that of astringent and it treats horse disease. This medicine has a peculiar smell, chews the sting, and it is irritating. The taste is spicy and the quality is cool. Ulgum communicates the muscles and helps the blood circulation to cure menstrual cramps, menstrual irregularities and flank pain, treats blood clots, nosebleeds, papules, cleanses the mind, removes the victims' parts and promotes bile secretion and treats gallstones. Pharmacological actions have been reported to reduce bile secretion, excretion, and the formation of nodules in the coronary arteries. Ulugum has been recorded since BC and has been used as a coloring agent for dyes and foodstuffs and is the raw material for Indian curry. In Japan, it is used as a coloring material for radishes, and it is cultivated as a special crop in Okinawa, Japan, known as a world longevity village, and is used as a health food.

Peppermint ( Mentha arvensis var. Piperascens) is a perennial herbaceous perennial plant of the monocotyledonous plants of the monocotyledonous plant. It is also known as the yomikomura, bunchach, indancho, and minta . It grows in damp fields. It is 60 ~ 100cm high. The stem has a square cross section and hairs on the surface. The leaves are faced with a leaf with a bag, and the edges are serrated. On the surface of the leaf there is an oil gland where the oil is secreted. Most of the essential oil is stored in this oil gland. Peppermint oil is a pale yellow liquid which is obtained by drying stem and leaf of peppermint and separating the essential oil contained in it by steam distillation. It is a liquid which contains free menthol, which is generated when the essential oil is cooled to below 4 ℃, , And it is also known as de-balun oil. Menthol is used as an antiseptic, analgesic, stimulant, nasal agent, insect repellent, or as a refreshing agent or fragrance for toothpaste, jam, candy, cosmetics, and tobacco. There are many kinds of mints that are made from peppermint oil. They are often called different names depending on the raw species. The oriental species ( M. arvensis ) has a high menthol content in refined oil and refers to it when referring to peppermint oil. The essential oil of Peppermint, a Western species, is called peppermint oil and is rich in aroma and good quality. Other spearmint oil from spearmint produced in North America and Europe, and penny royal oil from Spain's Penny Royal mint. Peppermint oil is widely used in soft drinks, toothpaste, fragrance of confectionery, medicines and the like.

Dandelion (陳皮) is the mature peel of citrus unshiu ( citrus unshiu ) or related plants in Korea. In Japan, tangerine (Citrus unshiu) and byeonggam (Citrus reticulata) into the dermis, are using gyulgam (Citrus tachibana) to gyulpi (橘皮) in China stipulates the byeonggam (Citrus reticulata) and the cultivar as the dermis. The dermis is said to be better in the long term, and the redder the better, so the name of Hongpie and Dermis is formed. The fruit is called tangerine (Tachibana), the leaf is tachibana (橘 葉), the yellow part where the inside of the fruit bark is removed is called 柑 紅 (橘), the seed is called 橘 核 (橘 核) . It has a peculiar odor, is slightly irritating, has a spicy flavor, and is warm. The dermis cleanses the gums and strengthens the function of the spleen, treating only the abdominal cavity, trimming, vomiting, nausea, indigestion, sluggishness, lingering symptoms, and thin stools. Seawater, sputum and diuretic action. It is a medicine that the effect of medicine increases with the reading aloud, mahwang, dandelion, sour oil, Pharmacological action has been reported to cause the digestive tract stimulation, digestion promotion, geomorphic, anti-ulcer, antidiarrheal secretion, hypertension, elevated blood pressure, antiallergic, bile secretion promotion, uterine smooth muscle suppression, Its appearance is unevenly shaped shell, outer side is yellowish red, dark tan, and there are many small concave marks due to loss. The inside is white or pale grayish brown, and the vagina is light and easy to crumble. Other names include 皮 귀 귀 귀)))))))))))))))))))))))))))))))))))))))))))))))))))))))))))), .

Licorice is called ural licorice and curly licorice. It grows in Russia (Siberia), Iran, Afghanistan, Pakistan, China (Gansuk province, Shin Kangseong), Mongolia and grows in Korea. European licorice is distributed in Southern Europe, Central Asia and China. As a variant of European licorice, there are Russian licorice, Persian licorice, and Iran licorice, but not medicinal. In Korea and Japan, roots and stem parts of licorice ( Glycyrrhiza uralensis Fischer), European licorice ( Glycyrrhiza glabra L.) or other plants are peeled or peeled. In China, dried roots of licorice ( Glycyrrhiza uralensis Fischer: licorice), licorice ( Glycyrrhiza glabra L) and licorice ( Glycyrrhiza inflata Batal: 果 甘草) are used. Licorice has a unique odor, sweet taste, harmonizes the toxicity of all medicines, and has the effect of showing good efficacy. In addition to this, licorice regulates the heat and fraud of the book, makes good communication of all blood vessels, and strengthens muscles and bones. Pharmacological action is effective in detoxification, hepatitis, urticaria, dermatitis, eczema. Jinhae, genomes, muscle relaxation, diuretic, anti-inflammatory action and inhibits peptic ulcer. The active ingredients of licorice include glycyrrhizin, glabraic acid, liquiritin which is a flavanone glycoside, neuriculitin, rhamnolcuritin, chalcones glycoside isocyanuric acid, And dozens of other flavonoids and coumarins such as hernia, umbelliferone, malic acid, benzoic acid, ferulic acid, and saccharides.

Jujube is the fruit of a jujube, also called jujube or wood grain. It is used for cookies, medicinal use, and as jujube, jujube tea, jujube vinegar, and jujube porridge. Honey juice as a processed product is popular in China, Japan and Europe. In one room, it is used as diuretic, tonic, and emollient. Examples of the active ingredient of jujube include fructose, sucrose, glucose, rhamnose and organic acids such as succinic acid, malic acid, wine acid, succinic acid and glycolic acid. Examples of inorganic salts include potassium, iron, cobalt, . In addition, it is rich in proteins, amino acids, ascorbic acid, cadecine, flavonoids and the like.

On the other hand, although the preference for health functional foods for relieving stress using various herbal medicines is increasing, the efficacy and effects thereof have not been scientifically proved, and medicines based on herbal medicines have been abused or misused abusively.

Accordingly, the inventors of the present invention completed the present invention upon confirming that the extract of the herbal medicine mixture containing the mackerel, mackerel, sardine, ointment, peppermint and peppermint has the stress relieving effect while studying the health functional food for relieving stress.

Meanwhile, herbal medicine extracts containing licorice and jujube such as licorice and jujube in Korea Patent No. 475197, Korean Patent Publication No. 2005-0015214, Korean Patent No. 278204 and Korean Patent No. 204166 have stress relieving effect However, since the kind of herbal medicine contained in the composition of the prior art or the compounding ratio thereof is different from that contained in the extract of the herbal medicine mixture of the present invention, the present invention can be said to be an invention having a different composition from the prior art have.

Korean Registered Patent No. 475197 (Health supplement food effective for relieving stress and its manufacturing method, registered on Feb. 25, 2005) Korean Unexamined Patent Publication No. 2005-0015214 (herbal composition for relieving stress by using grape as a main material, published Feb. 21, 2005) Korean Registered Patent No. 278204 (Functional Stress Relief Drink, registered on October 17, 2000) Korea Patent No. 204166 (Anti-Stress Composition, published on Mar. 26, 1999)

It is an object of the present invention to provide a health functional food for stress relieving containing an extract of a crude drug mixture, and more particularly to a health functional food containing an extract of a crude drug mixture containing gingko nigrescens, And to provide a health functional food for relieving stress.

The present invention relates to a health functional food for relieving stress containing an extract of a herbal medicine mixture, and more preferably to a health food for stress relief comprising an extract of a herbal medicine mixture, which comprises a safflower, a safflower, an ointment, Functional foods.

The herbal medicine mixture may contain 50 to 200 parts by weight of herbs, 20 to 100 parts by weight of herbs, 20 to 100 parts by weight of herbage, and 20 to 100 parts by weight of peppermint, based on 100 parts by weight of germania.

In addition, the herbal medicine mixture may contain one or more herbal medicine selected from the group consisting of 5 to 30 parts by weight of dermis, 5 to 30 parts by weight of licorice and 5 to 30 parts by weight of jujube based on 100 parts by weight of gum.

The extract of the crude drug mixture can be prepared by extracting a herbal medicine mixture comprising hamburger, herb, herb, herb, and peppermint with water, C1 to C4 lower alcohol or a mixed solvent thereof.

In another aspect, the present invention provides a process for the preparation of an extract of a crude drug mixture comprising germ, spinach, ointment, corn, and peppermint,

(1 step) preparing a herbal mixture by mixing 100 parts by weight of germ, 20 to 100 parts by weight of herbal oil, 20 to 100 parts by weight of herbal oil, and 20 to 100 parts by weight of peppermint.

(Step 2) Mixing the crude drug mixture of the above-mentioned one step with an extraction solvent having a total weight of 1 to 10 times the total weight of the crude drug mixture and extracting the mixture at 70 to 130 ° C for 1 to 10 hours; And

(Step 3) Extracts of herbal medicine mixtures containing gingko leaves, herbs, ointments, herbs and peppermint can be prepared through the steps of removing the solid content of the extracts of the two processes and collecting the filtrate.

Hereinafter, the present invention will be described in detail.

The extract of the crude drug mixture may be used in the form of a concentrate obtained by concentrating the extract at a reduced pressure or a dried product thereof when a C1 to C4 lower alcohol or a mixed solvent with water is used. In addition, in the case of extracting with water, the filtrate from which the solid content has been removed can be immediately used, and it can be used in the form of a concentrated product or a dried product thereof.

The extract of the crude drug mixture may be isolated by known methods used for separation and extraction of plant components, such as extraction with an organic solvent (alcohol, ether, acetone, etc.), partition with hexane and water, Or may be fractionated or purified by a suitable combination method.

The chromatography can be carried out using silica gel column chromatography, LH-20 column chromatography, ion exchange resin chromatography, medium pressure liquid chromatography chromatography, thin layer chromatography (TLC), silica gel vacuum liquid chromatography, and high performance liquid chromatography.

The dosage of the health functional food of the present invention will depend on the age, sex, body weight of the subject to be treated, the specific disease or pathological condition to be treated, the severity of the disease or pathological condition, the route of administration and the judgment of the prescriber. Dosage determinations based on these factors are within the level of ordinary skill in the art and generally the dosage ranges from 0.01 mg / kg / day to approximately 2000 mg / kg / day, based on the concentrated dry matter of the herbal mixture. A more preferable dosage is 1 mg / kg / day to 500 mg / kg / day. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

The present invention also provides a health functional food for relieving stress containing an extract of the herbal medicine mixture and a pharmaceutically acceptable food-aid additive. The health functional food of the present invention includes forms such as tablets, capsules, pills, and liquids. Examples of the foods to which the extract of the present invention can be added include various foods, beverages, gums, tea, vitamins , And health functional foods.

Meanwhile, the health functional food for stress relief containing the extract of the herbal medicine mixture of the present invention may be provided as a form easily sterilized and packaged in a pouch form.

The present invention relates to a health functional food containing an extract of a herbal medicine mixture, which comprises an extract of a herbal medicine comprising ginger root, ginger root, persimmon oil, ganoderma, and peppermint. The composition containing the extract of the herbal medicine mixture is excellent in stress relieving effect, , Can be easily used as a food for stress-relieving modern people weary of daily life.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flow chart showing a process for producing an extract of a crude drug mixture of the present invention. FIG.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Rather, the intention is to provide an exhaustive, complete, and complete disclosure of the principles of the invention to those skilled in the art.

EXAMPLES Example 1 Preparation of Extracts of Herbal Drug Mixtures Including Gastropung, Herb, Ointment,

The crude drug mixture of Table 1 below was mixed with 3 kg of water and extracted with a herbal medicine extractor at 120 ° C for 9 hours. The solids were removed to obtain an extract of each herbal mixture. At this time, the total weight of the herbal medicine mixture was 330 g, and a herbal medicine mixture of 50-200 parts by weight, 20-100 parts by weight of herbal oil, 20-100 parts by weight of herbage and 20-100 parts by weight of peppermint, . In some embodiments, 5 to 30 parts by weight of dermis, 5 to 30 parts by weight of licorice, and 5 to 30 parts by weight of jujube are mixed with the herbal medicine mixture based on 100 parts by weight of gum.

Condition Country (g) (G) Bream (g) Gulgum (g) Mint (g) Dermis (g) Licorice (g) Jujube (g) Total (g) Example 1-1 100 170 20 20 20 0 0 0 330 Examples 1-2 100 50 100 40 40 0 0 0 330 Example 1-3 100 50 40 100 40 0 0 0 330 Examples 1-4 100 50 40 40 100 0 0 0 330 Examples 1-5 100 100 35 30 35 30 0 0 330 Examples 1-6 100 100 35 30 35 0 30 0 330 Examples 1-7 100 100 35 30 35 0 0 30 330 Examples 1-8 100 100 35 30 35 15 15 0 330 Examples 1-9 100 100 35 30 35 15 0 15 330 Example 1-10 100 100 33 33 33 15 8 8 330

≪ Comparative Example 1 > Preparation of comparative extracts >

An extract of the crude drug mixture was prepared in the same manner as in Example 1, and the extract for comparison was prepared by referring to Table 2 below.

Condition Country (g) (G) Bream (g) Gulgum (g) Mint (g) Total (g) Comparative Example 1-1 30 150 50 50 50 330 Comparative Example 1-2 50 0 100 100 80 330 Comparative Example 1-3 100 130 0 0 100 330 Comparative Example 1-4 100 0 130 0 100 330 Comparative Example 1-5 100 150 0 80 0 330 Comparative Example 1-6 100 0 230 0 0 330 Comparative Example 1-7 0 100 0 230 0 330 Comparative Example 1-8 0 0 100 0 230 330

< Experimental Example  1. Confirmation of stress relieving effect ①>

For the stress-relieving effect experiment, the extracts of Example 1 and Comparative Example 1 were orally administered to mice at 4 weeks of age using ICR mice (mouse: 25 ± 3 g, male) for 4 weeks for 7 weeks in each group 21 were treated with sterilized distilled water only, 7 animals were unstimulated as untreated control group, 7 animals were used as physical stress control group, and 7 animals were used as psychological stress control group). Seven out of the untreated control group mice that did not take the extract were subjected to electrical shock of 2 mA for 1 hour from 10:00 to 11:00 for 3 days (120 seconds after 10 seconds of shock) 1 &lt; / RTI &gt; and the extract of Comparative Example 1 were observed in a socio-psychological stress box during the same period (causing psychological stress for one hour per day). After 3 days of stress induction, blood was collected and corticosterone was measured in the plasma to evaluate the stress relieving effect. For blood sampling, the mice were anesthetized with ether, and the blood was collected from the heart, left at low temperature for 2 hours, and centrifuged at 700 xg for 10 minutes. Next, the centrifuged supernatant was stored at -70 ° C in a complete blood cell count (Green Cross) treated with 0.05 ml of sodium heparin per 1.0 ml of blood, and then cryostomized with corticosterone ) Was used for the analysis.

The content of corticosterone in blood plasma was measured by Sliber (1958). 1 ml of plasma and 3 ml of petroleum ether were mixed and vigorously shaken for 30 seconds. After centrifugation, the solvent layer was discarded. The purified plasma was diluted with 5 ml of distilled water, and 15 ml of methylene chloride (methylene chloride) for 30 seconds. The extract was added to a tube containing 1 ml of 0.1 N NaOH solution, shaken for a short time and centrifuged. After centrifugation, the alkali layer was discarded and 10 ml of methylene chloride was added to a tube containing 2 ml of 30 N sulfuric acid. After centrifugation for 1 ~ 2 minutes, the supernatant layer was discarded and 2 ml of the lower layer was left at room temperature for 30 ~ 90 minutes. Then, the value of O.D. was measured at excitation 470 nm and emission 530 nm. 20 mg of the corticosterone standard was dissolved in 5.0 ml of ethanol, and a standard calibration curve was prepared using corticosterone diluted to 0.1 - 0.5 μg with distilled water to quantitate the content (㎍ / dl plasma) of each sample.

Condition Content of corticosterone (μg / dl plasma) Physical Stress Load Control 33 Psychological stress load control 27 Example 1-1 16 Examples 1-2 15 Example 1-3 16 Examples 1-4 15 Examples 1-5 14 Examples 1-6 16 Examples 1-7 14 Examples 1-8 15 Examples 1-9 13 Example 1-10 14 Comparative Example 1-1 21 Comparative Example 1-2 23 Comparative Example 1-3 24 Comparative Example 1-4 22 Comparative Example 1-5 23 Comparative Example 1-6 22 Comparative Example 1-7 23 Comparative Example 1-8 24 Non-stressed and untreated controls 12

Table 3 shows that the extract of the crude drug mixture of Example 1 of the present invention had remarkably better stress relieving effect than that of the crude drug mixture of Comparative Example 1 and exhibited a stress index similar to that of the non- .

EXPERIMENTAL EXAMPLE 2. Confirmation of stress relieving effect (2)

From Sep. 2012, the extracts were administered to 10- to 60-year-olds for 7 days for each extract of Example 1 and Comparative Example 1, for 2 days after a meal for 20 days, three times a day for 3 days, and 20 days The state of the body and mind was shown by the 5-point spot method (5 points: excellent, 4 points: excellent, 3 points: normal, 2 points: poor, 1 point: very poor) in Table 4 below. For this, sleepiness effect, helplessness improvement effect, fatigue feeling improvement effect, stress relieving effect were confirmed.

Condition Sleep effect Helplessness improvement effect Fatigue improvement effect Stress relieving effect Example 1-1 3.7 3.8 3.9 3.8 Examples 1-2 3.9 3.9 4.0 3.8 Example 1-3 3.8 4.0 3.9 3.9 Examples 1-4 4.2 3.9 4.0 4.0 Examples 1-5 3.9 3.9 4.2 4.1 Examples 1-6 3.8 4.2 4.1 4.1 Examples 1-7 4.2 4.3 4.2 3.9 Examples 1-8 4.3 4.2 4.3 4.2 Examples 1-9 4.0 4.2 3.8 4.3 Example 1-10 4.2 4.1 4.0 4.2 Comparative Example 1-1 2.5 2.2 2.0 2.4 Comparative Example 1-2 2.5 2.3 2.4 2.1 Comparative Example 1-3 1.8 1.9 2.2 2.0 Comparative Example 1-4 2.1 1.9 2.4 2.0 Comparative Example 1-5 2.0 2.1 1.8 2.2 Comparative Example 1-6 2.2 1.8 2.1 1.9 Comparative Example 1-7 1.9 2.2 2.0 1.8 Comparative Example 1-8 1.5 1.9 1.7 2.1

* 5 points: Excellent, 4 points: Excellent, 3 points: Average, 2 points: Poor, 1 point: Very poor

As shown in Table 4, the extract of the herbal medicine mixture of the present invention had a sleeping effect, reduced helplessness, improved fatigue and stress relieving effect.

< Experimental Example  3. Toxicity test>

Experimental Example 3-1. Acute toxicity

In order to investigate the toxicity of the herbal mixture (lyophilized product) of Example 1-10 of the present invention to the animal body in an acute (within 24 hours) when an excessive amount of the extract of lyophilized mixture of the present invention was taken in a short period of time and to determine the mortality rate, Respectively. Twenty ICR mice were injected into each of the control and experimental groups. The control group was administered only PEG-400 / tween-80 / ethanol (8/1/1, v / v / v) and the experimental group was treated with the PEG-400 / tween -80 / ethanol (8/1/1, v / v / v) for oral administration. After 24 hours of administration, the respective mortality rates were examined. As a result, it was confirmed that all the mice survived in the control group and the experimental group administered with the herbal mixture extract of Example 1-10 of the present invention at a concentration of 2 g / kg / day.

Experimental Example 3-2. Organ organs toxicity test in experimental group and control group

In the long-term toxicity test, the extract (lyophilisate) of the crude drug mixture of Example 1-10 of the present invention was administered to C57BL / 6J mice (10 mice per group) for 8 weeks at each concentration. In order to investigate the effects on the organs (tissues) of animals, the experimental group to which the extract of the crude drug mixture of Example 1-10 of the present invention was administered and the PEG-400 / tween-80 / ethanol (8/1/1, v / 8 weeks after the administration of v / v alone, the blood was collected and the concentration of glutamate-pyruvate transferase (GPT) and blood urea nitrogen (BUN) in the blood was measured using a Select E (Vital Scientific NV, Respectively. As a result, GPT, which is known to be related to hepatotoxicity, and BUN, which is known to be related to renal toxicity, showed no significant difference compared to the control group. In addition, liver and kidney were cut from each animal, followed by a general tissue section preparation, histological observation with an optical microscope, and no abnormalities were observed in all tissues.

&Lt; Formulation Example 1 >

Formulation Example 1-1. Manufacture of tablets

200g of the extract (lyophilized product) of the crude drug mixture of Example 1-10 of the present invention was mixed with 175.9g of lactose, 180g of potato starch and 32g of colloidal silicic acid. To this mixture was added a 10% gelatin solution, which was pulverized and passed through a 14-mesh sieve. This was dried, and a mixture obtained by adding 160 g of potato starch, 50 g of talc and 5 g of magnesium stearate was made into tablets.

Formulation Example 1-2. Injection preparation

1 g of the herbal mixture extract (lyophilized product) of Example 1-10 of the present invention, 0.6 g of sodium chloride and 0.1 g of ascorbic acid were dissolved in distilled water to make 100 ml. This solution was placed in a bottle and sterilized by heating at 20 DEG C for 30 minutes.

<Formulation Example 2: Food Preparation>

Formulation Example 2-1. Manufacture of cooking seasonings

The lyophilized mixture extract (lyophilized product) of Example 1-10 of the present invention was added to the cooking seasoning at 1% by weight to prepare a cooking sauce for health promotion.

Formulation Example 2-2. Manufacture of flour food products

(Lyophilized product) of the herbal mixture of Examples 1-8 of the present invention was added to wheat flour at 0.1 wt%, and bread, cake, cookies, crackers and noodles were prepared using the mixture to prepare health promotion foods Respectively.

Preparation Example 2-3. Manufacture of soups and gravies

The health enhancing soup and the juice were prepared by adding 0.1% by weight of the extract (lyophilized product) of the herbal mixture of Example 1-10 of the present invention to the soup and the juice.

Formulation Example 2-4. Manufacture of dairy products

The lyophilized mixture extract (lyophilized product) of Example 1-10 of the present invention was added to milk in an amount of 0.1% by weight, and various dairy products such as butter and ice cream were prepared using the milk.

Formulation Example 2-5. Vegetable juice manufacturing

0.5 g of the herbal mixture extract (lyophilized product) of Example 1-10 of the present invention was added to 1,000 ml of tomato juice or carrot juice to prepare vegetable juice for health promotion.

Formulation Example 2-6. Manufacture of fruit juice

0.1 g of the herbal mixture extract (lyophilized product) of Example 1-10 of the present invention was added to 1,000 ml of apple juice or grape juice to prepare health promotion fruit juice.

Claims (6)

A health functional food for stress relief characterized by containing as an active ingredient an extract of a herbal medicine mixture containing a sweet potato, a sweet potato, a herb, an ointment, a herb, and a peppermint. The method according to claim 1,
Wherein said herbal mixture is mixed with 50 to 200 parts by weight, 20 to 100 parts by weight of herbal oil, 20 to 100 parts by weight of herbage, and 20 to 100 parts by weight of peppermint, food. 3. The method according to claim 1 or 2,
Wherein the herbal medicine mixture contains one or more herbal medicine selected from the group consisting of 5 to 30 parts by weight of dermis, 5 to 30 parts by weight of licorice and 5 to 30 parts by weight of jujubes based on 100 parts by weight of germlings. food. 3. The method according to claim 1 or 2,
Wherein the extract of the crude drug mixture is prepared by extracting a herbal medicine mixture comprising mackerel, sardine, sardine, oyster, herring and peppermint with water, C1 to C4 lower alcohol or a mixed solvent thereof. food. (1 step) preparing a herbal mixture by mixing 100 parts by weight of germ, 20 to 100 parts by weight of herbal oil, 20 to 100 parts by weight of herbal oil, and 20 to 100 parts by weight of peppermint.
(Step 2) Mixing the crude drug mixture of the above-mentioned one step with an extraction solvent having a total weight of 1 to 10 times the total weight of the crude drug mixture and extracting the mixture at 70 to 130 ° C for 1 to 10 hours; And
(Step 3) removing the solid content of the extract of the two steps and collecting the filtrate;
Wherein the extract is a mixture of two or more of the following ingredients. 6. The method of claim 5,
Wherein the extraction solvent of the two steps is selected from water, C1 to C4 lower alcohols or a mixed solvent thereof.

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