KR101444210B1 - A system for indoor inspection of ocean organism - Google Patents

Abstract

The present invention relates to an indoor system for inspecting marine products, wherein the impact of a sea environment on several sorts of marine organisms can be experimented and inspected indoors. More specifically, a water storing tank which is a normal control group, an experimental tank mounted with a pH sensor and a bubble dispersing unit, and another experimental tank mounted with a DO sensor and a bubble dispersing unit are disposed on a water tank table. A running water-type environment is constituted so that a particular quantity of seawater is continuously supplied and discharged through the water storing tank and experimental tanks. CO_2 gas, N_2 gas, or O_2 gas is directly supplied to each of the bubble dispersing units installed in the experimental tanks through a sensor feedback mode using an MFC and a controller. The pH value and dissolve oxygen concentration of the sea water stored in the experimental tanks are maintained at required levels. Accordingly, the impact of marine acidification, which is the pH value of the seawater, on marine organisms and the impact of the dissolve oxygen concentration on marine organisms are simultaneously inspected, so that more particular and systematic environmentally-biological data can be obtained. The running water-type supplying of seawater and the direct feeding technique of gas through experimental tanks are applied, so that a system can be simply formed and more efficient and rational experiment and inspection are possible. The supplying paths of seawater and gas through each of the experimental tanks are constructed through individual single lines, so that confusions caused when the system is operated can be prevented. Also, when the system is broken or defected, the system can be rapidly treated.

Description

Background Art [0002] A system for indoor inspection of ocean organism

The present invention is characterized in that a water tank to be a normal control group, an experimental tank equipped with a pH sensor and a bubble dispersing machine, another experimental tank equipped with a DO sensor and a bubble dispersing machine are arranged on a water tank table, In addition, the bubble disperser installed in each experimental tank is used to directly supply CO ₂ gas, N ₂ gas or O ₂ gas by sensor feedback method by MFC and controller. The pH value and dissolved oxygen concentration of the seawater stored in each experiment group are kept constant at the required levels. In addition to the effects of ocean acidification, ie, the pH value of seawater on marine organisms, the concentration of dissolved oxygen The results of this study are summarized as follows. First, It relates.

Carbon dioxide (CO ₂ ), a major contributor to global warming caused by human industrial activities, absorbs one-third of its total emissions in the atmosphere while the other third is absorbed into land forests and soils, It is known to be absorbed by the oceans, which can be interpreted as a delay in global warming, owing to the fact that the oceans, which make up a large part of the Earth, reduce the total amount of carbon dioxide by 33%.

However, in recent years, oceanic acidification, in which the pH value of the ocean decreases due to excessive absorption of carbon dioxide into the ocean, is rapidly proceeding, and the pH of the ocean surface is reduced from about 8.179 to 8.104 from 1751 to 1994 And that the acidity of the oceans will increase 150% by 2050.

This oceanic acidification is highlighted as a serious environmental problem because of the health and growth of marine organisms such as shellfish or fish, some of which are composed of calcareous bodies, including plant and zooplankton and corals, which form the basis of the food chain This is because it affects the entire food chain of the marine ecosystem and ultimately seriously damages human survival and economic activity.

Worldwide awareness of the seriousness of ocean acidification and the development of alternative energy sources such as sunlight and wind power will reduce CO2 emissions by reducing the use of fossil fuels such as petroleum and coal, while reducing the effects of ocean acidification on various marine life. The research is underway to find a biological genetic trait that is resistant to ocean acidification and to compete to gain a foothold to secure future fisheries resources.

However, the biggest problem in studying and experimenting on the effects of marine acidification on various marine organisms is that the supply of professional manpower and customized testing equipment has not been properly achieved, As a result, the diversity and reliability of the data obtained through experiments are also very low, as well as the economical efficiency and effectiveness are very low due to the ever-changing sea conditions.

The seawater discharged from the acidification aeration tank and the aeration aeration tank is mixed at different ratios at different ratios and stored separately in the storage tank according to the pH concentration. Then, the sea water in the storage tank is sent to the culture tank, The present invention relates to a method and apparatus for analyzing the effect of marine acidification on various marine organisms in accordance with the pH concentration, by means of which the present applicant has filed a patent application No. 130782 10-1174487).

However, it was possible to analyze the effect of ocean acidification on marine organisms by supplying the different pH values to each culture tank and supplying them to the corresponding culture tank. However, in addition to marine acidification, There is a problem in that it is impossible to simultaneously experiment and analyze the influence of dissolved oxygen deficiency on various marine life as another environmental threatening factor.

In other words, if the thermocline is strongly formed by excessive organic matter (nutrients containing nitrogen or phosphorus) from the land, the ozone layer in the summer is not smooth, and dissolved oxygen in the bottom layer is depleted. Although large numbers of fishermen such as shellfish lacking mobility are killed in large quantities due to the presence of vacant oxygenated water bodies (poorly oxidized water bodies: water bodies with a dissolved oxygen concentration of 3 mg / L or less), it is necessary to conduct experiments, Nevertheless, in the case of the earlier application, the system was operated with a focus on ocean acidification only.

In addition, in the case of the prior art system, the arrangement of the aeration tank, the storage tank and the culture tank and the piping line connecting them are intricately intertwined. In order to control the flow rate of gas and seawater, MFC (mass flow controller) Valves and the like must be installed for each pipeline, there is a problem that the entire system including the pipeline for supplying and distributing seawater and the control route for the controller becomes very complicated.

In addition, for the uniform mixing of acidic and basic seawater, an agitator should be installed for each storage tank, and an exponential test environment in which the seawater is stored in the culture tank for a certain period of time is required for the cultivation of marine life The oxygen supply device must be additionally provided for each culture tank for the supply of dissolved oxygen, so that there is also a problem that the cost of the system installation and operation and maintenance is somewhat increased.

In addition, the pH of the seawater stored in the aeration aeration tank, the pH of the seawater stored in the basifying aeration tank, and the pH of the seawater stored in each reservoir need to be individually controlled, and the seawater discharged from the aeration tank The operation of the system for separating and storing the required pH concentration in the storage tank is also somewhat complicated, which may cause mistakes or crosstalk according to the erroneous operation of the system during the experiment, May be unnecessarily delayed.

Korean Patent No. 10-1174487

SUMMARY OF THE INVENTION The present invention has been made in order to solve the problems of the prior art as described above. The indoor sea bioassay system according to the present invention comprises a water tank serving as a normal control tank, an experimental tank equipped with a pH sensor and a bubble dispersing device, Another experimental tank equipped with a dispersing device is placed on a water table, and a running water environment in which a certain amount of seawater is continuously supplied and discharged through the water tank and the experiment tank is created, By supplying CO ₂ gas, N ₂ gas or O ₂ gas directly to the dispersing device by sensor feedback method using MFC and controller, the pH value and dissolved oxygen concentration of the seawater stored in each experimental tank are kept constant to the required level, In addition to the effects of acidification, ie, the pH value of seawater on marine life, the effect of dissolved oxygen concentration on marine life To make more specific and systematic environmental biological data and to construct the system more easily by applying the direct injection method of gas through the water supply and experimental tank of the sea water, In addition, it is necessary to construct sea water and gas supply routes through individual test vessels as individual single lines to prevent confusion caused by the operation of the system, and also to be able to cope with system malfunctions or faults promptly. This is a technical problem.

As a means for solving the above technical problems, an indoor marine biological assay system according to the present invention is characterized in that an experimental tank in which a pH sensor is mounted and another experimental tank in which a DO sensor is mounted and a water tank in a normal control tank are installed on a water table A bubble disperser is installed in each of the experiment vessels, and a seawater supply line extending from the seawater supply pipe through the distribution valve is connected to the upper end of the water tank and the experimental tank, and the water tank and the sea water supply line by an amount of water that flows to the outside of the water tank, the drain valve for the composition of the flow-through environment to be automatically discharged, and the installation connected to the drainage line, the pH test set of bubble dispersing device a sensor is mounted, the gas injection lines from the CO ₂ tank and MFC And a gas distribution line extending through the gas diffusion line The pH disperser is connected to a gas distribution line extending from the N ₂ / O ₂ tank through a gas injection line and an MFC. The pH sensor and the DO sensor are connected to the controller by a sensor cable. And the MFC is controlled based on the data input from the DO sensor.

As a more specific application example, one or two sets of the water storage tanks are provided on the water tank table, and two to four water tank groups are provided on the water tank table in which the experimental tank in which the pH sensor is mounted and the DO sensor are mounted, the valve in accordance with the water storage tank and the experiment number of sets of the installation is arranged on the outlet side of the water supply line adjacent to the water tank table, the MFC is a gas distribution with a gas distributor and the gas distributor for the N ₂ / O ₂ for CO ₂ in accordance with the test number set of installation Wherein the main valve of the gas distributor for CO ₂ is connected to the CO ₂ tank by a gas injection line, and the main valve of the gas distributor for CO ₂ is connected to the CO ₂ tank by a gas injection line Meanwhile, the main valve of the N ₂ / O ₂ gas distributor is connected to the N ₂ / O ₂ tank by a gas injection line, and each of the MFCs is connected by a gas relay line _One for each of the distribution valves of the CO ₂ gas distributor and the N ₂ / O ₂ gas distributor, and the gas distribution lines are individually extended from the respective MFCs and connected to the bubble distributor of the corresponding experimental tank .

In addition, the pH sensor and the DO sensor are installed so as to penetrate the water tank cover of the experiment tank in the vertical direction with the waterproof pack interposed therebetween, and the water tank cover is provided with a sensor mount for supporting a pH sensor or a DO sensor, The sensor mount includes a sensor bracket fixed to the upper surface of the water tank cover in a vertical direction, a mount plate for closely contacting the pH sensor or the DO sensor to the sensor bracket side, and an assembly bolt for assembling the mount plate to the sensor bracket A plurality of lid attaching units are installed at predetermined intervals on the outer periphery of the water storage tank and the test tank, and the lid attaching unit includes a hinge bolt hingedly connected to the upper end of the water tank, And a mounting knob which is assembled to the hinge bolt in a nut-like manner, and a hinge ball And is characterized in that the insertion recess is formed that is, the set of experiments has been installed over-pressure outlet tank cover, the feed pump is characterized in that it is installed in parallel to the sea water supply to the coronary 2 or 3 as.

According to the present invention, the effect of ocean acidification, that is, the influence of the pH value of seawater on marine life, the effect of dissolved oxygen concentration on marine life, Thus, it is possible to obtain more specific and systematic environmental biological data as compared with the case of the prior art which focuses only on ocean acidification.

In addition, it is possible to simplify the overall structure of the system including the pipeline for the sea water supply and the control route for the controller by applying the water supply of the sea water through the water tank and the experimental tank and the direct injection method of the gas through the tank At the same time, it provides a more efficient and rational experiment through the feedback control of MFC by sensor and controller.

In addition, it is effective to provide a cheaper and more economical system by applying only the sensor mechanism and the bubble dispersing device to each experimental tank. By constructing the single supply line of the sea water and the gas through each experimental tank, Thereby minimizing the operator's mistakes or crosstalk that may occur during the process, and enabling a quick response to a malfunction or failure of the system.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a front view showing an installation state of an indoor marine biological assay system according to the present invention. FIG.
Fig. 2 is a side view of Fig. 1; Fig.
3 is a cross-sectional view of the main portion of the experimental tank used in the present invention.
Figure 4 is a plan view of Figure 3;
5 is an internal structural view of the gas distribution unit used in the present invention.
6 is a piping diagram showing a seawater supply and discharge path applied to the present invention.
7 is a piping diagram showing a CO ₂ gas supply and control path according to the present invention.
8 is a piping diagram showing a N ₂ / O ₂ gas supply and control path according to the present invention.

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

1 and 2, an indoor marine biological assay system 1 according to the present invention includes an experiment vessel 4 on which a pH sensor 5 is mounted and another vessel on which a DO sensor 6 is mounted A water tank 3 serving as a normal control group is placed on the water table 2 and a CO ₂ tank 10 and an N ₂ / O ₂ tank 11 are connected to one side of the water table 2, .

2, the water tank 2 is provided with a three-stage frame structure using an angle frame or the like. However, considering the number of applications of the water storage tank 3, the experiment tank 4, The shape of the water tank 2 can be changed arbitrarily and a drainage passage 18 is formed on the bottom side of the room to drain the water discharged from the water storage tank 3 and the experimental tank 4 to the outside, It is needless to say that a drain pipe or the like may be applied in addition to the pipe 18.

A controller 13 for controlling the system and a gas distribution unit 12 are provided in the wall of the room and a seawater supply pipe 17 introduced from the outdoor side to the indoor side is connected to the lower front side of the water tub 2 And the gas distribution unit 12 is connected to the controller 13 by a cable line 13a with a mass flow controller (MFC) as a basic element, and the sea water supply pipe 17 Is closed.

A seawater supply line 7 extending from the seawater supply pipe 17 through the distribution valve 17b is connected to the upper end of the water storage tank 3 and the experiment tank 4, A drain valve 9 is connected to the drain line 9a at the lower end of the tank 4 for providing a drainage type environment for automatically discharging the amount of seawater flowing into the seawater supply line 7 to the outside of the water tank.

As shown in FIGS. 3 and 4, the experimental tank 4 is a sealed vessel in which a water tank cover 4b is assembled with a waterproof packing 4c interposed therebetween at the upper end of the water tank 4a A pH sensor 5 or a DO sensor 6 is vertically penetrated through the water tank 4a with a waterproof packing 4c interposed therebetween and a bubble disperser 8 .

The seawater supply line 7 is connected to a seawater inlet 7a provided at the upper end of the water tank 4a and the bubble disperser 8 is connected to the water tank 4a through a mounting port 20 provided in the water tank cover 4b, And is connected to the gas distribution lines 12a and 12b inserted into the tank 4a and connected to the penetration portions of the seawater supply line 7 and the gas distribution lines 12a and 12b, It is needless to say that the airtightness must be provided at the installation site of the valve 9.

As a further matter, the water tank cover 4b is provided with a sensor mount 19 for supporting the pH sensor 5 or the DO sensor 6. The sensor mount 19 includes a water tank cover 4b, A mounting plate 19b for bringing the pH sensor 5 or the DO sensor 6 into close contact with the sensor bracket 19a side and a mounting bracket 19b fixed to the mounting plate 19a, And an assembly bolt 19c for firmly assembling the sensor bracket 19a with the sensor bracket 19a.

The use of the sensor mount 19 can stably mount the pH sensor 5 or the DO sensor 6 on the water tank cover 4b, The lower sensor tip position of the pH sensor 5 or the DO sensor 6 can be easily adjusted to the required depth by separating the sensor 19b and the mounting plate 19b, 4, as shown in FIG. 4, and a coupling nut is fastened to the rear end of the assembly bolt 19c.

In addition, a plurality of lid attaching devices 21 are radially provided at a predetermined interval in the outer periphery of the test tank 4, and the lid attaching device 21 has a hinge (not shown) at the upper end of the water tank 4a A hinge bolt 21a connected to the hinge bolt 21a and a mounting knob 21b fastened to the hinge bolt 21a in a nut-like manner. In the periphery of the water tank cover 4b, (21c) are formed.

When the lid attaching device 21 as described above is applied to the test tank 4, the respective lid attaching devices 21 are always suspended in the tank tank 4a. Therefore, the water tank tank 4a is provided with the water tank tank 4b It is possible to perform the operation of mounting the water tank cover 4b more quickly and to remove the water tank cover 4b from the water tank tank 4a by unscrewing the mounting knob 21b and pulling the hinge bolt 21a downward The separation operation of the water tank cover 4b can be further easily performed.

The gas component supplied through the bubble dispersing device 8 is accumulated in the inside of the test tank 4 so that the test tank 4 And prevents the pumping force of the seawater through the seawater supply line 7 from being hindered by the internal pressure of the test tank 4. As a result, It is also advantageous in terms of creating a smooth flow of water.

The storage tank 3 is connected to a pH sensor 5 or a DO sensor 6 and a sensor mount 19 for mounting them in the experimental tank 4 shown in Figs. 3 and 4, a bubble distributor 8, A mounting port 20 for installing the gas distribution lines 12a and 12b connected thereto and a structure similar to that of the experimental tank 4 in a state in which the overpressure discharge port 16 is omitted, It is preferable that the water tank tank 4a and the water tank cover 4b constituting the tank 3 and the experimental tank 4 are made of transparent acrylic material.

1 and 2, the gas bubbler 8 in the experimental tank 4 equipped with the pH sensor 5 is supplied with the gas containing the gas injection line 10a and the MFC from the CO ₂ tank 10 from the gas distribution line (12a), the N ₂ / O ₂ tank 11, the bubble dispersing device (8) of the experimental tank 4 is connected to the installation and, DO sensor 6 is mounted extending through a dispensing unit (12) A gas injection line 11a and another gas distribution line 12b extending through the gas distribution unit 12 including the MFC are connected.

The pH sensor 5 and the DO sensor 6 are connected to the controller 13 by sensor cables 5a and 6a and the controller 13 is connected to the pH sensor 5 and the DO sensor 6 And the gas distribution unit 12 including the MFC is controlled on the basis of the input data. Values inputted from the pH sensor 5 and the DO sensor 6 are numerically expressed on the outer surface of the front side of the controller 13 And an emergency switch 15a are provided in the system to prevent the malfunction of the system or the malfunction of the system when the malfunctioning switch 15a is connected to the system, Thereby stopping the overall operation of the system.

Referring to FIGS. 1 and 2, two water basin tanks 2 are provided on the water tank 3, and an experiment vessel 4 equipped with a pH sensor 5 and a DO sensor 6 In the case of the water tank 3 serving as a normal control group, only one water tank 3 may be provided, and an experiment tank 4 in which the pH sensor 5 is mounted 4 and the DO sensor 6 can be installed from at least two to five or more.

However, it is not preferable to install five or more experimental tanks (4) in consideration of the circumstances of the narrow indoor space and the economical efficiency of the experiment. Therefore, as shown in Figs. 1 and 2, The supply path of the seawater through the seawater supply pipe 17 and the supply path of the gas from the CO ₂ tank 10 and the N ₂ / O ₂ tank 11 in accordance with the installation state of the water storage tank 3 and the test tank 4, The supply route of the gas through the unit 12 is constructed as a single single line.

6 shows the supply path of the seawater applied when two reservoirs 3 and a total of eight experiment vessels 4 are installed. The distribution valve 17b is connected to the reservoir 3 and the experimental tank 4, A total of ten seawater supply lines 7 extending from each of the distribution valves 17b are disposed in the water storage tank 3 in correspondence to the number of the water storage tank 2 and the outlet side of the adjacent sea water supply pipe 17, ) And the test tank 4 side, and then connected to the seawater inlet 7a of the tank.

In addition, the drainage line 9a connected to the drainage valve 9 at the lower end of each of the reservoir 3 and the experimental tank 4 extends toward the drainage passage 18 in the room, and in order to avoid the complexity of the drawing, The drainage lines 9a are integrated into a single line but each drainage line 9a is also a single single line as in the case of the sea water supply line 7 and only the adjacent drainage lines 9a It is also possible to integrate into the line of.

In addition, when the water tank 3 and the experimental tank 4 are used as reference, the seawater is flowed at a relatively small flow rate of about 0.5 L (liter) per minute. However, It is advantageous that the two or three supply pumps 26 are installed in parallel on the seawater supply pipe 17 so as to be alternately operated and the removal of foreign matter is performed on the inlet side of the sea water supply pipe 17 It is desirable to install a seawater filter 17C for the water.

5, 7, and 8, a total of eight experiment vessels (4) including four experimental vessels 4 on which the pH sensor 5 is mounted and four experimental vessels 4 on which the DO sensor 6 is mounted The gas distribution unit 12 is provided with eight MFCs 24 corresponding to the number of the experiment vessels 4 installed in the gas distribution unit 12, The gas distributor 22 for CO ₂ and the gas distributor 22 for N ₂ / O ₂ .

Each of the MFCs 24 is connected to a relay board 25 having a function of adjusting voltage and current and a signal transmission function by a connection cable 25a and the relay board 25 is connected to a cable line 13a, Each of the gas distributors 22 is a small-sized gas storage tank having a cylindrical shape, and one main valve 22a is provided at the lower end thereof, and four A dispensing valve 22b is provided.

The main valve (22a) for the CO ₂ gas distributor 22 comprises a main gas injection line connected to the installation and the CO ₂ tank 10 by (10a) On the other hand, the N ₂ / O ₂ gas distributor 22 for the valve (22a) is a gas inlet line and to the installation connections (11a) N ₂ / O ₂ tank (11) by each of the MFC (24) is a gas distributor 22 for the CO ₂ by a gas transit line 23 One for each of the distribution valves 22b of the N ₂ / O ₂ gas distributor 22 and one for each of the distribution valves 22b of the N ₂ / O ₂ gas distributor 22.

Therefore, the gas distribution line (12a), (12b) are each MFC extending from 24 to the one individual bar that is installed connected to the bubble dispersing device (8) of the experiment bath (4), CO ₂ supply the gas distribution lines The upper four lines and the lower four lines are connected to the same gas distribution line 12a (12b) as shown in FIG. 5 so that the gas distribution line 12a for supplying N ₂ / O ₂ and the gas distribution line 12b for supplying N ₂ / So as to escape to the outlet side of the gas distribution unit 12.

It is preferable to use a high-pressure hose having a small diameter of about 1 to 2 mm for the gas injection lines 10a, 11a, the gas relay line 23 and the gas distribution lines 12a, 12b, The seawater supply line 17 may be a corrosion resistant metal or plastic pipe having a diameter of about 2 to 5 cm and the seawater supply line 7 and the drain line 9a may be made of a corrosion- The CO ₂ tank 10 and the N ₂ / O ₂ tank 11 are high pressure tanks storing liquefied gas or compressed gas.

The pH concentration of the seawater stored in each experimental tank 4 is 7.2, 7.4, 7.8, and 8.0 through the feedback control of the CO ₂ gas supply amount by the pH sensor 5, the controller 13, and the MFC 24 And the dissolved oxygen concentration of the seawater stored in each of the experiment vessels 4 is controlled by the DO sensor 6 and the N ₂ gas supply amount by the controller 13 and the MFC 24 It is desirable to maintain the level of 3.5, 4.5, 5.5, and 6.5 ppm by feedback control.

Therefore, in the present invention, the experiment of culturing the marine organisms in a water-soluble manner under the conditions of acidification condition and dissolved oxygen using the N ₂ tank together with the CO ₂ tank 10 is given priority, and the condition of the basic sea water ( _{2) In} case of experiment, O ₂ tank should be replaced with N ₂ tank when experiment is required under the overcondition condition of dissolved oxygen. The cultured organism to be put into the experimental tank (4) is typical of fish such as fish, shellfish or sea cucumber Any marine organism that requires experimentation can be put into each experimental group (4) as a culture organism.

According to the present invention, the effect of ocean acidification, that is, the influence of the pH value of seawater on marine life, and the influence of dissolved oxygen concentration on marine life can be simultaneously tested and verified in the room, And more specific and systematic environmental biological data can be obtained as compared with the case of the prior art which focuses only on the environment.

In addition, by applying the water supply of the seawater through the water tank 3 and the experiment tank 4 and the direct injection of the gas through the tank 4, the control line for the sea water supply piping and the controller 13 It is possible to simplify the overall configuration of the system including the MFC 24 and to perform more efficient and rational experimentation only through the feedback control of the MFC 24 by the pH sensor 5 and the DO sensor 6 and the controller 13 do.

It is also possible to provide a cheaper and more economical system by applying only the sensor mechanism and the bubble disperser 8 to each of the experimental vessels 4 and to separately supply the sea water and the gas supply channels through the respective vessels 4 By constructing with a single line, it is possible to minimize operator errors and crosstalk that may occur during the experiment, and to be able to cope with system malfunction or failure quickly.

1: Marine biological assay system 2: Tank table
3: Water tank 4: Experiment tank 4a: Water tank
4b: Tank cover 4c: Waterproof packing 5: pH sensor
6: DO sensor 5a, 6a: sensor cable 7: sea water supply line
7a: Seawater inlet 8: Bubble disperser 9: Drain valve
9a: Drain line 10: CO ₂ tank 11: N ₂ / O ₂ tank
10a, 11a: gas injection line 12: gas distribution unit 12a, 12b: gas distribution line
13: Controller 13a: Cable line 14: Indicator
15: Operation lamp 15a: Emergency switch 16: Overpressure outlet
17: Seawater supply pipe 17a, 22a: Main valve 17b, 22b: Distribution valve
17c: Seawater filter 18: Drain line 19: Sensor mount
19a: Sensor bracket 19b: Mounting plate 19c: Assembly bolt
20: mounting port 21: lid mount 21a: hinge bolt
21b: mounting knob 21c: incision groove 22: gas distributor
23: gas relay line 24: MFC 25: relay board
25a: connection cable 26: supply pump

Claims (6)

A plurality of water tanks serving as hermetically sealed vessels are dispersed and arranged on the water tank 2 and a seawater supply pipe 17 having a supply pump 26 and a main valve 17a is connected to each of the water tanks, In an indoor marine biological assay system (1) in which the effect of a marine environment on various marine life can be experimentally and assayed by installing a gas bubbling dispersing device (8) for forming an ecological environment in the water tank, ,
The water tank is divided into a test tank 4 in which the pH sensor 5 is mounted and another test tank 4 in which the DO sensor 6 is mounted and a water tank 3 as a normal control tank. , And a bubble disperser (8) is installed in each of the experiment vessels (4)
The experiment tank 4 in which the pH sensor 5 is mounted and the experimental tank 4 in which the DO sensor 6 is mounted are installed in the water tank 2 on the water tank 2, Two to four vessels are respectively installed on the water tank 2,
A seawater supply line 7 extending from a seawater supply pipe 17 through a distribution valve 17b is connected to the upper end of the water storage tank 3 and the experimental tank 4, A drain valve 9 is connected to the drain line 9a for forming a drainage type environment in which the amount of seawater flowing into the seawater supply line 7 is automatically discharged to the outside of the water tank,
A gas distribution line 12a extending from the CO ₂ tank 10 through the gas injection line 10a and the MFC 24 is provided in the bubble dispersing device 8 of the test tank 4 equipped with the pH sensor 5 And the gas bubble expander 8 of the experimental tank 4 equipped with the DO sensor 6 is extended from the N ₂ / O ₂ tank 11 through the gas injection line 11a and the MFC 24 A gas distribution line 12b is connected,
The pH sensor 5 and the DO sensor 6 are connected to the controller 13 by sensor cables 5a and 6a and the controller 13 is connected to the pH sensor 5 and the DO sensor 6 And the MFC (24) is controlled based on the input data. The water treatment system according to claim 1, wherein the dispensing valve (17b) is disposed at an outlet side of the sea water supply pipe (17) adjacent to the water tank table (2) according to the number of the water tank (3) 24 are installed to form a gas distribution unit 12 together with the gas distributor 22 for CO ₂ and the gas distributor 22 for N ₂ / O ₂ in accordance with the number of the test vessels 4,
The gas distributor 22 is provided with one main valve 22a and two to four distribution valves 22b and the main valve 22a of the CO ₂ gas distributor 22 is connected to a gas injection line (10a) that is installed connected to the CO ₂ tank 10 by the other hand, the main valve (22a) of the N ₂ / O ₂ gas distributor (22), by the gas injection line (11a) N ₂ / O ₂ tank (11)
Each of the MFCs 24 is connected to the distribution valve 22b of the CO ₂ gas distributor 22 and the distribution valve 22b of the N ₂ / O ₂ gas distributor 22 by the gas relay line 23 Characterized in that the gas distribution lines (12a) and (12b) are individually connected to the bubble distributor (8) of the experiment vessel (4), one by one from each MFC (24) Biological assay system. The water tank (4) according to claim 1 or 2, wherein the water tank (3) and the experimental tank (4) are provided with a water tank cover (4b) 6 are installed so as to penetrate the water tank cover 4b of the test tank 4 in the vertical direction with the waterproof pack kings 4c interposed therebetween,
A sensor mount 19 for supporting the pH sensor 5 or the DO sensor 6 is provided on the water tank cover 4b and the sensor mount 19 is mounted on the upper surface of the water tank cover 4b A mounting plate 19b for closely contacting the pH sensor 5 or the DO sensor 6 to the sensor bracket 19a side and a mounting plate 19b for mounting the mounting plate 19b to the sensor bracket 19a, And an assembling bolt (19c) for assembling the bioassay (19a). 4. The apparatus according to claim 3, wherein two to eight lid-attaching devices (21) are provided at regular intervals on the outer periphery of the water storage tank (3) and the test tank (4)
The lid mounting unit 21 includes a hinge bolt 21a hingedly connected to the upper end of the water tank 4a and a mounting knob 21b mounted on the hinge bolt 21a in a nut- ,
And an incision groove (21c) into which a hinge bolt (21a) is inserted is formed on a peripheral edge of the water tank cover (4b). The indoor sea bioassay system according to claim 3, wherein an overpressure outlet (16) is installed in the water tank cover (4b) of the test tank (4). The indoor sea bioassay system according to claim 1 or 2, wherein the supply pumps (26) are installed in parallel on the sea water supply pipe (17) in two or three.

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