KR101435331B1 - Feed supplement having suppresive activity against avian influenza virus and foot and mouth disease virus and, feed composition comprising the same - Google Patents

Abstract

The present invention is a feed additive or a feed composition containing nano-liquid sulfur as an active ingredient. The feed additive or the feed composition can prevent diseases such as hyperlipidemia or arteriosclerosis by suppressing body fat accumulation during livestock feeding to suppress an oversupply of cholesterol, neutral fat, etc. in plasma. Moreover, the feed additive or the feed composition can prevent avian influenza and foot-and-mouth disease by strengthening the immune system.

Description

[0001] The present invention relates to a feed additive having an avian influenza and foot-and-mouth disease inhibitory activity and a feed composition containing the feed additive,

The present invention relates to a feed additive having an avian influenza and foot-and-mouth disease suppressive activity and a feed composition containing the feed additive. More particularly, the present invention relates to a feed additive for inhibiting the excess of cholesterol and triglyceride To a feed additive or feed composition which prevents diseases such as hyperlipemia and arteriosclerosis and further provides a preventive effect against avian influenza and foot-and-mouth disease through the enhancement of immunity.

In recent years, communicable diseases such as foot-and-mouth disease, PRRS, and infectious diseases such as avian flu and swine flu, which are common infectious diseases, have caused social problems.

The foot-and-mouth disease virus is a very important disease for livestock. It is classified as A class in Office International des disease and it belongs to the livestock infectious disease in Korea as well. It can be infected by all animals with split hooves, such as cows, pigs, sheep, goats and deer. The disease is very contagious and in the early stages of the infection, there are blisters on the lips, tongue, gums, nose and hooves Sudden increase in body temperature, appetite, etc., symptoms such as the appearance of acute deaths.

In addition, highly pathogenic avian influenza (AI) is also classified as Class A by the International Bureau of OIE (OIE) and as a domestic livestock epidemic in Korea, with a high mortality rate of over 95%. In addition, swine influenza (SI) is an influenza type A (H1N1 or H3N2 type), but it is still in the category 2 livestock infectious disease in Korea. However, cross infection with human has been confirmed, .

In addition to direct damages caused by various viruses, countries that are affected by such viruses are also affected by international sanctions such as failure to export animal and livestock products to non-developing countries.

In recent years, large-scale livestock disposal work has been carried out in Korea due to foot-and-mouth disease, bird flu, etc., resulting in many side effects such as leachate contamination. As part of efforts to prevent these viruses from spreading to other regions, we have been putting a lot of money and manpower into the work to prevent all vehicles entering and leaving the road. Despite these efforts, however, as has been revealed recently in domestic foot-and-mouth disease, the current disinfection system has exposed a loophole that can not effectively block the spread of viruses.

So far, a lot of studies have been made on the anti-proliferative agent, preventive and therapeutic agent against foot-and-mouth disease or avian flu virus, but it is often harmful to the human body of an animal and its effect is also unclear. It is urgent to develop a feed additive having an excellent preventive effect.

Accordingly, the present invention has been devised to solve the above-mentioned problems, and it is an object of the present invention to prevent the diseases such as hyperlipidemia and arteriosclerosis by suppressing the excess of cholesterol and triglyceride in the plasma by suppressing the anti- And to provide a preventive effect against bird flu or foot and mouth disease through immunity enhancement.

According to an aspect of the present invention,

(1) Feed additives containing nano-liquid sulfur as an active ingredient.

(2) The feed additive according to claim 1, further comprising far-infrared photon water.

(3) The feed additive according to claim 1, further comprising a sulfur powder.

(4) The feed additive according to claim 3, wherein 5 to 20% by weight of a sulfur powder and 0.1 to 0.2% by weight of nano-liquid sulfur are added.

(5) The feed additive according to claim 1, which contains at least one lyophilized powder or extract selected from the group consisting of leek, garlic and onion.

(6) The feed additive according to claim 1, wherein the leek is fermented leek.

(7) A feed composition containing a feed additive according to any one of claims 1 to 6.

(8) A method for inhibiting avian influenza and foot-and-mouth disease in domestic animals, the method comprising feeding nano-liquid sulfur and far-infrared photon water to livestock.

As described above, the feed additive or the feed composition according to the present invention prevents the accumulation of anti-obesity or fat accumulation when fed to livestock and prevents excessive diseases such as hyperlipidemia and arteriosclerosis by inhibiting excessive plasma of cholesterol and triglyceride, Furthermore, it provides a preventive effect against avian influenza and foot and mouth disease through immunity enhancement.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is an overall system configuration diagram for feeding a feed additive according to the present invention to livestock; FIG.

Hereinafter, the contents of the present invention will be described in more detail.

In the present invention, the livestock to which the feed additive is to be fed includes all birds (for example, cattle, pigs, sheep, goats, deer, etc.), and birds such as chickens and ducks.

The present invention includes a feed additive containing nano-liquid sulfur as an active ingredient. In the present invention, nano-liquid sulfur, which is obtained by subjecting a volatile sulfur powder with toxicity to toxicity to a ball-mill, a basket-mill or a nano-mill, may be subjected to ultra-fine pulverization. At this time, preferably, the legal sulfur can be prepared by the method disclosed in Korean Patent No. 10-1070993.

Examples of the method for producing the nano-liquid sulfur include those described in Korean Patent Laid-open Nos. 10-2011-0114846 and 10-2007-0111567, etc. In the present invention, the nano-liquid sulfur obtained by using such a method, It is enough to use sulfur.

The nano-liquid sulfur, which is an active ingredient of the feed additive according to the present invention, may be prepared by mixing nano-sulfur and water in a ratio (v / v) of 1: 500-1: 1000.

Further, the feed additive according to the present invention is added with far-infrared photon water. The far infrared ray photon number generally means that the irregularly arranged water is arranged in a hexagonal shape by making the arrangement uniform by using the apparatus described in Korean Patent Laid-Open No. 10-2012-0113842. At present, the far infrared ray photon number can be obtained by water obtained by a water purifier commercially available ELT-701 (trade name, Ebada Eco-friendly Co., Ltd., Korea).

The nano-liquid sulfur and the far-infrared photon number are preferably mixed at a ratio (v / v) of 1: 500 to 1: 1000.

The feed additive according to the present invention may further comprise a sulfur powder. By mixing turmeric powder with nano-liquid sulfur, it prevents the accumulation of anti-obesity or body fat when fed to livestock and suppresses excess of cholesterol, triglyceride and the like in plasma, thereby further enhancing the function of preventing diseases such as hyperlipemia and arteriosclerosis .

At this time, it is preferable to add 5 to 20% by weight of the sulfur dioxide powder to the feed additive and 0.1 to 0.2% by weight of the nano-liquid sulfur.

The feed additive according to the present invention preferably contains at least one lyophilized powder or extract selected from the group consisting of leek, garlic and onion, more preferably fermented leek.

The lyophilized powder or extract thereof functions to enhance the immune activity while enhancing the preventive function against the disease. The at least one lyophilized powder or extract selected from the group consisting of leeks, garlic, and onions is not particularly limited, but is preferably added in an amount of 0.1 to 0.5% by weight.

The feed additive according to the present invention having the above-described structure can be introduced into livestock using the liquid supply device as shown in FIG.

That is, water is injected into the ELT-701 water purifier 2 through the water supply part 1a of the main tank 1 to produce the far-infrared photon water. The water thus produced is supplied to the nano-liquid sulfur supply part (1b) and then fed into at least one or more auxiliary tanks (3) to be fed into beverages (4).

The nano-liquid phase sulfur is introduced from the sulfur tank 5 into the nano-liquid phase sulfur supply unit 1b of the main tank 1 so that the nano-liquid phase sulfur and the far-infrared photon number are 1: 500 to 1: v to be mixed by the control panel 6. Likewise, the control panel 6 may control the amount of the feed additive supplied to each of the auxiliary tanks 3 and the injection time. The feed additive supplied to the auxiliary tank 3 can be injected into the form of a drink in the form of a beverage or injected into the form of an additive for the feed or sprayed using a mist sprayer installed in a house, The closing time and the like can be similarly controlled by the control panel 6.

Hereinafter, the present invention will be described in more detail with reference to the following Examples and Experimental Examples. However, the following examples and experimental examples are illustrative of the present invention, and the content of the present invention is not limited by the following examples and experimental examples.

[Example 1]

The mixture of nano-liquid sulfur and far-infrared photons at a ratio of 1: 500 (v / v) was prepared as a feed additive.

[Example 2]

The same procedure as in Example 1 was carried out except that the nano-liquid phase sulfur and the far-infrared photon number were mixed at a ratio of 1: 1000 (v / v).

[Example 3]

The same procedure as in Example 1 was carried out except that 5 wt% of a sulfur-containing powder and 0.1 wt% of a nano-liquid sulfur were added to the feed additive.

[Example 4]

The same procedure as in Example 1 was carried out except that 20 wt% of the sulfurized powder and 0.2 wt% of the nano-liquid sulfur were added to the feed additive.

[Example 5]

Except that 0.1% by weight of freeze-dried powder of leek was added.

[Example 6]

The same procedure as in Example 3 was carried out except that 0.1 wt% of the lyophilized powder of garlic was added.

[Example 7]

The same procedure as in Example 3 was carried out except that 0.1% by weight of the lyophilized powder of onion was added.

[Example 8]

Fermented leek, garlic, and onion were added in an amount of 0.1% by weight based on the weight of the freeze-dried powder.

[Example 9]

The procedure of Example 5 was repeated except that 0.1% by weight of fermented leek was added instead of leek.

[Example 10]

The procedure of Example 8 was repeated except that fermented leek was added in an amount of 0.1 wt% instead of leek.

[Experimental Example 1] Weight gain and feed intake

Animal experiments were conducted to investigate the effect of nano-liquid sulfur on serum lipid changes in rats, and the feed additives of the above-described example cultivated in Haenam, Jeollanam-do were used. Five - week - old male Wister rats were immunized for 7 days before the experiment and were randomly divided into 5 groups according to body weight. The control group (CON) fed general feeds (Feedrap, Gyeonggi - do) and the control group (HFD) fed high - fat diet.

Weight change and total feed intake for 6 weeks were measured and the results are shown in Table 1.

Item Initial weight Final weight Weight gain Feed intake CON 181.2 345.5 164.3 20.8 HFD 181.3 367.0 185.7 19.6 Example 1 181.0 361.3 180.3 19.0 Example 2 181.0 342.5 161.5 19.7 Example 3 181.3 369.5 188.2 20.0 Example 4 181.5 370.2 188.7 20.1 Example 5 181.3 371.6 190.3 20.3 Example 6 181.4 372.0 190.6 20.7 Example 7 181.0 371.8 190.8 20.6 Example 8 181.2 371.5 190.3 20.4 Example 9 181.2 371.6 190.4 20.7 Example 10 181.4 371.4 190.0 20.5

As described above, the feed additives according to the present invention showed no significant difference in body weight change and body weight gain compared to the control group fed with the general diet and high fat diet.

[Experimental Example 2] Changes in serum lipids

Animal experiments were conducted to investigate the effect of nano-liquid sulfur on serum lipid changes in rats, and the feed additives of the above-described example cultivated in Haenam, Jeollanam-do were used. Five - week - old male Wister rats were immunized for 7 days before the experiment and were randomly divided into 5 groups according to body weight. The control group (CON) fed general feeds (Feedrap, Gyeonggi - do) and the control group (HFD) fed high - fat diet. The treatment groups (T, TS, S and AS) contained 10% by weight of the sulfurized powder, 10% by weight of the sulfurized powder, 0.19% by weight of the nano-liquid sulfur and 0.38% by weight of the nano- , And 10% by weight of a sulfur powder, 0.19% by weight of nano-liquid sulfur, and 0.1% by weight of fermented persimmon (TSA). The results are shown in Table 2.

Item Total cholesterol
(mg / dL) HDL cholesterol
(mg / dL) LDL cholesterol
(mg / dL) Neutral lipid
(mg / dL) CON 53.0 18.8 2.8 8.0 HFD 54.0 18.9 3.1 9.0 Example 1 38.0 21.1 2.0 6.0 Example 2 38.1 20.0 1.8 5.9 Example 3 32.0 19.4 1.6 5.4 Example 4 31.3 18.4 1.3 4.7 Example 5 31.2 17.9 1.4 4.6 Example 6 31.0 17.8 1.5 4.9 Example 7 30.8 17.0 1.2 4.1 Example 8 31.2 17.5 1.1 3.8 Example 9 31.1 17.0 1.2 3.5 Example 10 30.4 17.3 1.0 3.1

As shown in Table 1, in the group treated with nano-liquid sulfur, excellent results were obtained in the serum lipid changes in comparison with the normal control group and the comparative control group, and thus it was confirmed that diseases such as hyperlipemia and arteriosclerosis Of the patients.

[Experimental Example 3]

In order to confirm the inhibitory activity against the avian influenza virus H9N2 and the foot-and-mouth disease virus in the samples obtained in the above-mentioned respective Examples, the avian influenza virus H9N2 and foot-and-mouth viruses were mixed with the samples of the respective Examples and reacted for 1 hour. , And after one week, samples were taken from the fertilized eggs and the degree of viable virus was measured to the extent that plaque was formed on the medium. At this time, the same procedure was used except that amantadine was used instead of the sample of the above example as the control group.

Sample Avian influenza Foot-and-mouth disease Comparative Example × × Example 1 ○ ○ Example 2 ○ ○ Example 3 ◎ ◎ Example 4 ◎ ◎ Example 5 ◎ ◎ Example 6 ◎ ◎ Example 7 ◎ ◎ Example 8 ◎ ◎ Example 9 ◎ ◎ Example 10 ◎ ◎

*: Survival virus detection, ○: Survival virus trace amount detection, ◎: Survival virus detection not possible.

1: Main tank
2: Far infrared photonic water producing device
3: auxiliary tank
4: Congratulations
5: Sulfur tank
6: Control Panel

Claims (8)

Wherein the nano-liquid phase sulfur and the far-infrared photon number are in the range of 1: 500 to 1: 1000 (v (v)), / v). < / RTI > The feed additive for inhibiting avian influenza and foot-and-mouth disease. The feed additive for avian influenza and foot-and-mouth disease suppression according to claim 1, further comprising at least one lyophilized powder or extract selected from the group consisting of garlic and onion. A method for inhibiting avian influenza and foot and mouth disease in a domestic animal, the method comprising feeding the feed additive of claim 1 or 2 to livestock.

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