KR101399895B1 - A pharmaceutical comprising the extract of Acorus gramineus Soland for treating or preventing erectile dysfunction - Google Patents

Abstract

The present invention relates to a pharmaceutical composition for prevention and treatment of erectile dysfunction, which comprises a relaxation effect of the penile cavernosal smooth muscle via the NO-cGMP pathway and a pharmaceutical composition having relaxation effect of the penile cavernosal smooth muscle through hyperpolarization according to the opening of the K ⁺ channel .
The present invention relates to a pharmaceutical composition for prevention and treatment of erectile dysfunction, and relates to a composition containing an extract of Seokchangpo as an active ingredient.

Description

[0001] The present invention relates to a composition for preventing and treating erectile dysfunction which comprises extracts of Seokchangpo as an active ingredient,

The present invention relates to a pharmaceutical composition for prevention and treatment of erectile dysfunction, which comprises a relaxation effect of the penile cavernosal smooth muscle via the NO-cGMP pathway and a pharmaceutical composition having relaxation effect of the penile cavernosal smooth muscle through hyperpolarization according to the opening of the K ⁺ channel .

The present invention relates to a pharmaceutical composition for prevention and treatment of erectile dysfunction, and relates to a composition containing an extract of Seokchangpo as an active ingredient.

In general, Eerectile Dysfunction (ED) refers to a condition in which an erection is not sufficient to successfully perform sexual activity, or an erectile dysfunction can not be sustained for a sufficient period of time.

Here, it is the smooth muscle of the cavernous surface which plays a decisive role in the erection, since the blood flow is increased or maintained only when the smooth muscle is relaxed, so that the erectile force can be applied.

Specifically, the degree of erection is determined by the degree of relaxation of the smooth muscle caused by the combination of the relaxant materials acting on the smooth muscle of the penile artery and the cavernosal body and the shrinkable material. The endotherium-derived relaxing factor (EDRF) Nitric oxide (NO), acetylcholine, prostaglandin E1, and bradykinin.

The endotherium-derived constricting factor (EDCF) includes norepinephrine, EDCF, endothelin, and angiotensin II.

Recent studies have shown that Nitric Oxide (NO) is a powerful vasodilator (EDRF), and the role of nitric oxide in the relaxation of the corpus cavernosal smooth muscle is becoming more active.

Specifically, when the sexual impulse is transmitted through the nervous system, NO synthase (NO synthase, NOS) produces NO in the endothelial cell using L-arginine as a substrate.

This endothelium-derived nitric oxide converts soluble guanylate cyclase into smooth muscle cells to convert GTP (guanosine 5'-triphosphate) into cGMP (3 ', 5'-cyclic guanosine monophosphate) Thereby increasing the concentration.

The generated cGMP is that it stimulates the protein kinase G (PKG) of cGMP- specific protein enzyme inhibiting smooth muscle intracellular Ca ^{+ 2} influx and thus the relaxation of smooth muscle sensitivity to shrinkage factor of Ca ^{2 +} is reduced (NO-cGMP Mechanism of Penile Erection)

In addition to the NO-cGMP pathway, K ⁺ channels play an important role in the contraction and relaxation of the smooth muscle of the corpus cavernosum.

That is, if K ⁺ channel is active opening by the action of vascular relaxing factor, as the exit is K ⁺ were in existence in high concentration in the cell out of the cell ileonaneunde of the polarization of the cell and out of simhaejyeo cell hyperpolarization, this reaction is Ca into the cytoplasm ^{2 +} is prevented from flowing, the smooth muscle is relaxed.

Here, there are at least four K ⁺ channel subtypes in the cells of the corpus cavernosal smooth muscle: ① calcium-sensitive K ⁺ channel, ② metabolically regulated K ⁺ channel, ③ fast transient A current, ④ delayed rectifier.

Among these K ⁺ channels, the calcium-sensitive channel and the K _ATP channel are most important. When these channels are opened, K ⁺ outflows from the cell, and positive charge in the cell is lost, resulting in hyperpolarization.

As described above, since the physiological phenomenon of penile erection is revealed and the pharmacological action and mechanism of the various pharmacological agents against the cavernosal smooth muscle are studied, efforts have been actively made to use the pharmacological agents having relaxation action in the smooth muscle for the erectile dysfunction treatment .

Viagra, a chemically synthesized substance, Vardenafil, and Tadalafil, a citrate drug product, is a synthetic derivative that acts as a competitive reaction material with cGMP, as a commercially available drug for erectile dysfunction, PDE-5 (phosphodiesterase-5) blocks cGMP-mediated single pathway and improves vascular smooth muscle relaxation by increasing cGMP concentration in the corpus cavernosum.

However, these drugs have a temporary effect, and side effects such as headache, facial flushing, indigestion and heart failure have been reported.

Therefore, there is a need for a drug that maintains natural erectile ability rather than one - time and compulsive erection, and has no side effects.

On the other hand, the relationship between impotence and cardiac health has been studied over a long period of time, and there is no clear evidence to date.

This is based on the fact that the arteries supplying blood to the penis can be blocked earlier than the larger arteries supplying blood to the heart, so that erectile dysfunction can be an early symptom of coronary artery disease.

In Korea, a variety of medicines such as ginseng, red ginseng, antler, gugija, corn oil, bokbunja, tortoise, and omija have been used as folk remedies for erectile dysfunction in Korea. However, studies on erectile dysfunction have been lacking in other medicinal plants.

The present invention relates to a composition for preventing and treating erectile dysfunction, which comprises an extract of Seokchangpo as an active ingredient.

Seok Changpo (Acorns, Acorns gramineus Soland) is the rootstock (rootstock, rootstock) of Seonchungpo and perennial herbaceous plant.

In oriental medicine, it is said that the nature is warm, the taste is very high, and it is said to be in the heart and the stomach (胃 经), and it takes care of the nihilism of 风 寒濕 邪It is known that it has the effect of controlling the circulation of the blood circulation, circulating the blood, releasing the wind, and removing the wetness.

Pharmacologically, it contains ingredients such as β-asarone, caryophyllene, α-humulene, and sekishone. It has sedative action, promotes secretion of digestive juices, inhibits abnormal fermentation of the stomach and at the same time relaxes the seizures of the intestinal smooth muscle .

Meanwhile, Korean Patent No. 0522176 discloses a sexual function composition containing about 47 natural products, which is disclosed in a composition component of Seok Changpo.

Korean Patent No. 0672950 discloses an extract of Seokchangpo for the prevention or treatment of ischemic heart disease.

As a summary, we can see that Seok Changpo is used as one of 47 kinds of natural products in relation to erectile dysfunction, and the use of Seok Changpo is focused on prevention and treatment of heart disease.

It is an object of the present invention to provide a composition for preventing, ameliorating and treating erectile dysfunction by improving vascular condition as a root cause of erectile dysfunction by not taking the concept of a one-time treatment but taking it continuously without causing side effects have.

An object of the present invention, NO-cGMP route and K ⁺ to pay attention to the corpus cavernosum smooth muscle relaxation effects of the passage opening, by measuring the corpus cavernosum relaxation effect of the White Rabbit, erectile dysfunction prevention, an effect of improving and treating significant Composition.

The present invention also provides a composition which is remarkably effective in preventing, ameliorating, and treating erectile dysfunction by measuring erectile efficacy of penile corpus cavernosum.

The present invention aims to solve the technical problem by providing a pharmaceutical composition for preventing and treating erectile dysfunction, which comprises extract of Seokchangpo as an active ingredient.

Further, the present invention provides a pharmaceutical composition for preventing and treating erectile dysfunction, characterized in that ethanol (volume 5 to 10 times the weight of the whole sample) is preferably used as a solvent for extraction of the seokchonpo. .

The present invention solves the technical problem by providing an agent for preventing and treating erectile dysfunction which is formulated into a pharmaceutical unit dosage form by adding an extract of Seokchangpo as an effective ingredient and adding a pharmaceutically acceptable carrier, excipient or diluent I want to.

Examples of the carrier, excipient and diluent include tosse, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, Vaginal cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

The pharmaceutical dosage forms may also be used in the form of pharmaceutically acceptable salts, and may be used alone or in combination with other pharmaceutically active compounds as well as in a suitable set.

In addition, when the extract of Seokchangpo is prepared, it may be prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, surfactants and the like which are usually used.

In addition, each of the above pharmaceutical dosage forms may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols or the like, oral preparations, suppositories, .

In the solid preparation for oral administration, at least one excipient such as starch may be prepared by mixing calcium carbonate, sucrose, lactose, gelatin or the like in the extract. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used.

The preparation for parenteral administration may include a sterilized aqueous solution, a non-aqueous solvent, a suspension, an emulsion, a freeze-dried preparation, and a suppository.

Examples of the non-aqueous solvent and the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like.

Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The preferred dosage of the extract of the present invention varies depending on the condition and the weight of the patient, the degree of disease, age, sex, drug form, route of administration and period of time, but can be appropriately selected by those skilled in the art.

However, for the desired effect, the extract of the present invention is preferably administered at 0.001 to 300 mg / kg, and the administration may be carried out once a day or divided into several times.

The dose is not intended to limit the scope of the invention in any way.

The extract of the present invention can be administered to mammals such as rats, mice, livestock, humans and the like in various routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, muscular, subcutaneous injection.

The present invention aims to solve the technical problem by providing a health functional food composition for preventing and treating erectile dysfunction by adding a food auxiliary additive to the extract of Seokchangpo.

Examples of foods to which the extract can be added include various foods, beverages, gums, tea, vitamin complexes, and health functional foods.

At this time, the amount of the extract in the food or drink may be 0.01 to 20% by weight of the total food, and the health beverage composition may be added in a proportion of 0.02 to 5 g, preferably 0.3 to 1 g based on 100 ml .

The health functional beverage composition of the present invention is not particularly limited to the components other than those containing the above extract, and may contain various flavors or natural carbohydrates as additional components such as ordinary beverages.

Examples of the above-mentioned natural carbohydrates are monosaccharides such as glucose, fructose; Disaccharides such as maltose, sucrose and the like and polysaccharides such as dextrins, cyclodextrins and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. In addition to the above, natural flavoring agents (tau martin, stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavorings.

The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention.

In addition to the above, the extract of the present invention can be used as a nutritional supplement, a vitamin, a mineral (electrolyte), a flavor such as a synthetic flavor and a natural flavor, a coloring agent and an aggravating agent (cheese, chocolate etc.), a pectic acid and its salt, Colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like.

In addition, the extracts of the present invention may contain flesh for the production of natural fruit juices and fruit juice beverages and vegetable beverages.

These components may be used independently or in combination. The proportion of such additives is not so critical, but is generally selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the extract of the present invention.

The pharmaceutical composition for prevention and treatment of erectile dysfunction diseases containing the extract of Seokchangpo as an active ingredient of the present invention can be used continuously without taking side effects to improve the vascular condition as a root cause of erectile dysfunction and prevent erectile dysfunction , ≪ / RTI > improvement and treatment.

The pharmaceutical composition for prevention and treatment of erectile dysfunction disease containing the extract of Seokchonpo extract of the present invention as an active ingredient has a remarkable effect of preventing, ameliorating and treating erectile dysfunction by performing relaxation action of the smooth muscle of the corpus cavernosum.

The pharmaceutical composition for prevention and treatment of erectile dysfunction disease containing the extract of Seokchangpo extract as an active ingredient of the present invention is useful for relaxation of the smooth muscle of the corpus cavernosum through the NO-cGMP pathway and relaxation of the smooth muscle of the penile corpus cavernosum through hyperpolarization according to the opening of the K ⁺ .

The pharmaceutical composition for prevention and treatment of erectile dysfunction diseases containing the extract of Seokchangpo extract of the present invention as an active ingredient has a remarkable effect of preventing erectile dysfunction which improves erectile function by increasing internal pressure of the corpus cavernosum and treating and treating it.

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a graph showing experimental results on relaxation effect of smooth muscle of cauliflower according to Experimental Example 1. Fig.
2 is a graph showing the results of experiments on NO-cGMP activity according to Experimental Example 2. FIG.
3 is a graph showing experimental results on cGMP generation effect according to Experimental Example 3. FIG.
4 is a graph showing experimental results on the K ⁺ channel activity effect according to Experimental Example 4. FIG.
5 to 7 are graphs showing ICP, ICP / MAP, and AUC measurement results for the internal pressure of the cavernosal corpuscle according to Experimental Example 5, respectively.

The terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms and the inventor may properly define the concept of the term in order to best describe its invention It should be construed as meaning and concept consistent with the technical idea of the present invention.

Therefore, the embodiments described in the present specification and the configurations shown in the drawings are merely the most preferred embodiments of the present invention and are not intended to represent all of the technical ideas of the present invention. Therefore, various equivalents It should be understood that water and variations may be present.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Example  One. Seokchangpo  Preparation of extract

400 g of Seokchangpo were immersed for one week at room temperature using an ultrasonic digester (100 W, 40 kHz) so that the sample could be extracted sufficiently to 5 to 10 times the weight of the total sample, preferably to 2 L of ethanol.

The extracts of Seokchonpo extracted for one week were first filtered through mung bean mud to remove moss and debris, and then filtered using a Watman NO.3 filter by vacuum filtration.

Finally, the filtered ethanol extract of Seokchangpo was concentrated by using a vacuum concentrator and then lyophilized to obtain a powdery composition (41.09 g).

Reference example  1. Experimental preparation

1-1. Experimental animal

New Zealand white rabbits (about 2 kg) were distributed from Samtako, Korea to the laboratory environment for 2 weeks.

The experimental animals were fed with solid feed and water at a temperature of 22 ± 1 ℃, humidity 55 ± 10%, dark / light for 12 hours.

All animal studies were conducted with the National Institutes of Health (NIH) guide for the care and use of laboratory animals (USA) and the Wonkwang University Laboratory Animal Ethics Committee (WKU09-102).

1-2. Reagent preparation

The acetylcholine (ACh), phenylephrine HCl (PE), N ^G -nitroarginine methyl ester (L-NAME), 1 H- [1,2,4] -oxadiazolo- [4,3-a] -quinoxalin 1-one (ODQ), indomethacin, glibenclamide, tetraethylammonium (TEA), atropine, (±) -propranolol HCl, 3-isobutyl-1-methylxanthine (IBMX), trichloroacetic acid Louis, MO).

1-3. Isolation of smooth muscle of cavernous cavernosum

The animals were anesthetized with ketamine (50 mg / kg i.v.) through a diaphragmatic vein of a healthy rabbit and sacrificed by cardiac hemorrhage. Then, the penis tissue was peeled and removed until it was branched into the lower part of the pubic bone.

Penile tissues were washed with 4 ° C Krebs buffer (NaCl 136.9 mM, KCl 5.4 mM, MgCl ₂ 6H ₂ O _{1.0 mM, CaCl 2 2.0 mM,} NaHCO 3 23.8 mM, and glucose 5.5 mM), and the corneal cavernosal tissue strips (2 × 2 × 6 mm) were prepared by carefully removing the cavernosal surface of the penis to prevent damage to the endothelial cells of the corpus cavernosal smooth muscle.

1-4. Measurement of relaxation effect

The isoflurane tissue sections were fixed in Krebs buffer at 37 ° C, saturated with 95% O ₂ - 5% CO ₂ gas. The isometric tension was measured using a force-displacement transducer (Grass FT 03, Grass Instrument, MA ) (Grass Model 7E, Grass Instrument, MA).

After 5 min of contraction with 1 μM of phenylephrine (PE), the stability of the endothelial cells of the penile cavernosal smooth muscle was measured by relaxation with 1 μM of acetylcholine (ACh), followed by washing three times with Krebs buffer .

In order to measure the relaxation effect of the ethanol extract of Seokchangpo on pretreatment of various blocking agents, the drug was pretreated for 20 minutes before shrinking with PE, followed by relaxation reaction with ethanol extract of Seokchangpo.

1-5. cGMP  And cAMP  Production amount measurement

The corpus cavernosum was excised and the corpuscle was excised and cultured in a kreps solution containing 95% O ₂ and 5% CO ₂ , pretreated with PE and IBMX for 5 minutes, treated with 5 minutes of drug and then treated with 6% trichloroacetic acid (TCA) and centrifuged at 13,000 rpm for 15 min. The supernatant was extracted three times with water saturated diethylether. The extract was concentrated with a Speed-vac concentrator and cGMP and cAMP were measured by radioimmunoassay.

1-6. Statistical processing

The significance of the test results was analyzed using the Sigma Plot 2010 program. The Student t-test or the one-way ANOVA test was used to determine the significance of the test results when the p-value was less than 0.05.

Experimental Example  One. Seokchangpo  Relaxation effect of extracts on smooth muscle of corpus cavernosum

1 is a graph showing experimental results on the relaxation effect of the smooth muscle of the penile cavernosa according to Experimental Example 1. (Each value shows mean SD (n = 4))

The smooth muscle of the penile corpus cavernosum was shrunk with 1 μM PE for 5 minutes, and then the extract of Seokchangpo (0.1 ug / ml to 0.3 mg / ml, EAG (Ethanol extract of Acorus gramineus Solan) according to Example 1) As a result of measuring the relaxation effect of the smooth muscle of the corpus cavernosum, the smooth muscle of the corpus cavernosum relaxed in a concentration dependent manner, and a relaxation effect of 96.48 ± 2.24% was observed at a concentration of 0.3 mg / ml.

Experimental Example 2 . Seokchangpo  Extract NO - cGMP  Active effect

2 shows experimental results on the NO-cGMP activity effect according to Experimental Example 2. (Each value shows mean SD (n = 4). ^# P <0.05, * p <0.01, ** P <0.001 , etc. vehicle group.)

L-NAME 0.1 mM, a non-specific NO (nitric oxide) inhibitor, and 10 μM ODQ, a guanylate cyclase inhibitor, were each pretreated for 20 minutes and shrunk to 1 μM PE. The extracts were administered in a concentration-dependent manner to measure the relaxation effect of the corpus cavernosum.

FIG. 2 shows that the extract of Seokchang extract significantly activates the NO-cGMP pathway in a concentration-dependent manner, and the NO-cGMP pathway is significantly inhibited when the NO inhibitor and the guanylate cyclase inhibitor are pretreated have.

Experimental Example 3 . Seokchangpo  Extract cGMP  Generation effect

. Figure 3 is a diagram showing experimental results for cGMP generated effect according to the Experimental Example 3 (Each value shows mean ± SD (n = 5) * p <0.001, ** P <0.001, vs. vehicle group; † p <0.01, etc. EAG (3 μg / ml) group.)

As a result of measuring the amount of cGMP and cAMP produced after direct administration of the extract of Seokchangpo extract according to Example 1 to the corpus cavernosum tissue section, the concentration of cGMP Respectively.

On the other hand, the amount of cAMP produced was not changed by the ethanol extract of Seokchangpo.

Therefore, it can be shown that Seokchangpo extract activates the NO-cGMP pathway in a concentration-dependent manner in the corpus cavernosum tissue.

Experimental Example 4 . Seokchangpo  The K of the extract ⁺  Channel effect

FIG. 4 is a graph showing experimental results on the K ⁺ channel activity effect according to Experimental Example 4. Each value shows mean SD (n = 4). * P <0.01, ** P <0.001, .)

10 μM tetraethylammonium (TEA), a non-selective K ⁺ channel inhibitor, and 10 μM glibenclamide, an ATP-sensitive K ⁺ channel inhibitor, were each pretreated for 20 min and shrunk with 1 μM PE for 5 min. The relaxation effect of the smooth muscle of cavernous cavernosum was measured.

FIG. 4 shows that the relaxation effect of the smooth muscle of the penile corpus cavernosum was significantly inhibited by the TEA pretreatment, and the relaxation effect was not significantly inhibited by glibenclamide pretreatment.

Experimental Examples 1 to 4 are summarized as follows. Experimental Example 1: The extract of Seokchonpo has the effect of relaxing the smooth muscle of the penile cavernosum in a concentration-dependent manner.

In addition, L-NAME, which is an inhibitor of nitric oxide synthesis, and ODQ, a soluble guanylate cyclase inhibitor, were measured after the pretreatment, and the relaxation effect on smooth muscle of corpus cavernosum was significantly inhibited by the extract of Seokchangpo. (Experimental Example 2)

In addition, the ethanol extract of Seokchangpo has an effect of increasing the production amount of cGMP in a concentration-dependent manner. (Experimental Example 3)

In addition, the TEA and the K _ATP non-specific K ⁺ channel blockers as inhibitors of the K ⁺ channel Pathway-selective blockers sulfonylurea drug glibenclamide was pretreated and the relaxation effect of Seokchonpo extract was significantly inhibited by TEA. (Experimental Example 4)

Therefore, it can be understood that the relaxation effect of the smooth muscle of the penile corpus cavernosum by the extract of Seokchang extract according to Example 1 occurs via the NO-cGMP system.

In other words, the extract of Seokchangpo can increase the synthesis of nitric oxide in the endothelial cells and activate the soluble guanylate cyclase to increase the production of cGMP, which can be considered to relax the smooth muscle of the cavernous cavernosal.

In addition, the relaxation effect of smooth muscle of corpus cavernosum by the extract of Seokchangpo extract is caused by activation of K ⁺ channel.

Reference example  2. Experimental preparation

2-1. Experimental animal

Sprague-Dawley (SD) male white rats weighing 380 g were distributed from Samtako, Korea and were adapted to the laboratory environment for 2 weeks.

The breeding of the experimental animals and the animal experiments were carried out in the same manner as described in Reference Example 1-1.

2-2. Reagent preparation

The reagent preparation was prepared in the same manner as described in Reference Example 1-2.

2-3. Measuring pressure inside the corpus cavernosum intracavernosal pressure  : ICP )

A healthy white rats weighing approximately 400-450 g were anesthetized with sodium pentobabital (50 mg / kg, i.v.) and cannulated with carotid artery and jugular vein, respectively, and carotid artery pressure was measured and injected into the jugular vein.

Then, the penis was incised and the penis was completely exposed. A 23 G needle was inserted into the right penis, and the bladder plexus was connected to an electrical stimulator, and electrical stimulation was performed at 5 min intervals at 10 V, 0.1 ms, 3 Hz and 5 Hz intensity And the pressure inside the penis was measured.

2-4. Statistical processing

The statistical treatment was carried out in the same manner as described in Reference Example 1-6.

Experimental Example 5 . Seokchangpo  The effect of the extract on the internal pressure of the corpus cavernosum

FIG. 5 is a graph showing the results of ICP measurement (saline: vehicle, sildenafil: positive control, each value shows mean ± SD (n = 6). * P <0.05, ** p <0.01, *** p <0.001 vs. saline group. FIG. 6 shows ICP / MAP measurement results (Each value shows mean SD (n = 6). * p <0.05, ** p < (P <0.05, ** p <0.01, *** p <0.001 vs. saline group, p <0.001 vs. saline group) Respectively.

ICP, ICP / MAP, and AUC were measured by injecting 30 mg / kg of salicylic acid (EAG), saline 0.9% NaCl, and sildenafil 1 mg / kg from the jugular vein at a rate of about 0.1 ml / min.

ICP (intracavernosal pressure) is the value when the pressure inside the corpus cavernosum reaches the maximum from the time of the electric stimulation. ICP / MAP is the value when the pressure inside the corpus cavernosum reaches its peak, The ratio of blood pressure divided by area under curve of cavernosometry (AUC) is calculated by calculating the area of increased pressure over one minute of electrical stimulation.

5 to 7, the values of the intracavalular pressure (ICP), the ICP / MAP, and the area under the curve (AUC) are shown, respectively. It can be seen that the extract of Seokchangpo increased the internal pressure in a concentration dependent manner.

It was also found that the pressure in the corpus cavernosum was significantly increased compared with the saline alone group.

Experimental Example 5 is summarized as follows. It can be seen that Seokchangpo extract has a local erectile improving effect in the corpus cavernosum.

In addition, the AUC value is a dose / clearance, indicating that the effect of the extract of Seokchangpo on the erectile function is remarkable.

In Experimental Examples 1 to 5 as a whole, the extract of Seokchonpo according to Example 1 has relaxation effect of the penile cavernosal smooth muscle through the NO-cGMP pathway, and relaxes the smooth muscle of the cavernous cavernous smooth muscle by causing the hyperpolarization by opening the K ⁺ It can be said that.

In addition, Seokchangpo extract has a local erectile effect and a sustained effect of erectile function.

Claims (5)

Seok Changpo (Acorns, Acorns The present invention relates to a pharmaceutical composition for preventing and treating erectile dysfunction, which comprises an extract of Gramineus Soland as an active ingredient.
The method according to claim 1,
Wherein the extract is obtained by extracting with ethanol.
The method according to claim 1,
Wherein the extract relaxes the smooth muscle of the penile cavernosum through the NO-cGMP pathway.
The method according to claim 1,
Wherein the extract has hyperpolarization through the opening of the K ⁺ channel to relax the smooth muscle of the penile cavernosum.
A health functional food composition for preventing or improving erectile dysfunction, wherein a food additive is added to an extract of Seokchangba ( Acorus gramineus Soland).

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