KR101397539B1 - Composition of Assorted feed having resistance of heat stress for broiler - Google Patents

Abstract

The present invention relates to a compounded feed composition for broilers having resistance to heat stress in broiler chickens, and a method for breeding broilers using the same to reduce heat stress in broilers. The compound feed composition for broilers according to the present invention stimulates the proliferation of the immune system to increase the secretion amount of the blood immunity substance and not only reduce the stress hormone corticosterone but also maintain the proper acidity of the cecum, By promoting the growth of microorganisms and inhibiting the growth of harmful microorganisms, it is possible to effectively reduce the heat stress of broiler chickens through the action mechanism that improves the growth ability of broiler chickens. In addition, the method of breeding the broiler according to the present invention is a method of feeding the diets having different compositions according to the growth stage of the broiler, and in particular, in order to minimize the heat stress due to the 12 hour lighting control and the metabolic heat generation in the broiler, , The heat stress of broiler chickens can be effectively mitigated. Therefore, when the compounded feed and the breeding method of the present invention are used, it is possible to improve the productivity of broiler chickens by raising the resistance ability of the broiler chicken to heat stress (immune cell activation, reduction of stress hormone, maintenance of cecal microflora, etc.) You can contribute.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for a broiler feeder having heat stress resistance,

The present invention relates to a compound feed composition for broiler chickens having resistance to heat stress and a method for breeding broilers to reduce heat stress.

Korea is located geographically in the mid-latitude temperate climatic zone, and the four seasons of spring, summer, fall, and winter are clear, but recently the abnormal weather pattern is divided into short winter and long summer. Due to global warming or urbanization, the average annual temperature has risen by about 1 ℃ from 1904 to 1990. According to the Meteorological Agency statistics, in the past 100 years, the winter in Seoul has been 30 days shorter and the summer 20 days longer do. Also, in summer, it is hot and humid due to the effect of hot and humid North Pacific. In July and August, the average humidity is around 80% nationwide. This high temperature with high relative humidity is a difficult problem in poultry farms that can lead to economic losses in Newcastle (ND) and other respiratory diseases. The damage is caused by a decrease in feed intake and a decrease in productivity Of course, severe cases may lead to massive deaths, and indirect damages such as decrease in the amount of body weight gain, decrease in egg yolk, decrease in fertilization rate, increase in early embryo mortality, decrease in quality and survival rate of practical chicks (Heungseong Feed Co., Ltd., Shim Jae Min, 'High Temperature Stress and Poultry').

Chickens are constant-temperature animals that need to keep their body temperature constant during their lifetime. When they are over three weeks old, their body temperature rises to 41.6 ° C. Unlike other animals, chickens have no sweat glands and have skin covered with feathers. When the temperature rises to 25 degrees Celsius or more, the body temperature rises. At this time, body heat is discharged to the outside to maintain body temperature.

However, it is generally difficult to maintain proper body temperature as the outside temperature is higher than 30 ℃ (warmth) and closer to the chicken body temperature (41 ℃). Therefore, it is necessary to open the mouth and open the mouth of the body through panting Is an important means of releasing body heat. When 1 gm of water is evaporated, it emits 540 kcal of body heat. Chickens exposed to high temperature (heat) physiologically increase the opening respiration rate to maximize body heat emission through evaporation. In the meantime, this gasping means that respiratory rate is increased, thereby promoting the reduction of carbon dioxide (CO2) in the blood, thereby causing respiratory alkalosis. As such, when the electrolyte balance is broken, the productivity of broiler chickens is decreased, And if it can not prevent the rise of body temperature despite this open breathing, it will accompany the coma.

High temperature (heat) stress also affects the humoral immune function of the chicken, reducing the effect of the vaccine. High temperatures above 36 ° C have a negative effect on cellular immunity. The nasal cavity of the chicken has a function to filter dust and germs in the house. The panting and the open breathing due to the high temperature can cause the secondary bacterial respiratory disease with the deterioration of the filtering function, Or more. In addition, when the chicken is exposed to heat for a long period of time, the release of corticosteroids increases and the requirement for vitamin C, folic acid, and vitamin B12 increases, leading to a relative deficiency and a decrease in the body's ability to synthesize vitamin Decrease in these deficiency symptoms will lead to a decrease in serum vitamins and electrolytes, which can eventually lead to delayed growth, egg production, poor egg quality, and even death.

Therefore, when breeding chickens in an environment of more than 30 ℃ (heat), it is necessary to increase the number of broiler chickens such as blood parameters abnormality, stress hormone increase, immune capacity decrease, Physiological changes can cause economic damage, and eventually, the increase in mortality can cause a drop in farm income.

However, to date, no measures have been established to prevent damages caused by broiler chickens under the environment of heat (over 30 ℃). Especially, in case of broiler chickens that can relieve high temperature (heat) Research is minimal.

Accordingly, the present inventors have studied an optimum compound feed composition capable of effectively mitigating the high temperature (heat) stress of broiler chicks raised in a warm (30 ° C or higher) environment, and in addition to the commonly used broiler feed, soybean oil, molasses , Methionine, lysine and vitamin C in a specific range can effectively alleviate the high temperature (heat) stress of the broiler under the high temperature environment of 33 ° C, thereby completing the present invention. In addition, the present inventors have also found that the feed of diets having different compositions according to the growth stage of broiler chickens, the heating of broiler chickens under the heating condition for 12 hours and the feeding of chickens at a specific time in order to minimize the heat stress due to the generation of metabolic heat The present inventors have completed the present invention by confirming that the stress can be effectively reduced.

Korean Patent Publication No. 10-2001-0027079

Accordingly, an object of the present invention is to provide a compounded feed composition for broiler chickens having a resistance to heat stress in broilers including soybean milk, molasses, methionine and lysine in a basic diet for broiler chickens.

Another object of the present invention is (a) a method for producing a broiler chick, which comprises feeding hatching chicks to a broiler base stock for 21 days after hatching, And (b) feeding broiler diets supplemented with soybean milk, molasses, methionine, lysine and vitamin C to a basic diet for broilers from the 21 days after the lapse of 21 days, The present invention provides a method of breeding a broiler having reduced heat stress in broiler chickens, which is characterized by performing 12-hour lighting adjustment (female to male 08: 00-20: 00, name 20: 00-08: 00)

In order to achieve the object of the present invention as described above, the present invention provides a compounded feed composition for broiler chickens having a resistance to heat stress in broiler chickens, which further comprises soybean oil, molasses, methionine and lysine in a basic diet for broiler chickens.

In one embodiment of the present invention, soybean milk, molasses, methionine, and lysine, which are additionally contained in the broiler base feed, are prepared by mixing 5 parts by weight of soybean oil, 2 parts by weight of molasses, 0.15 part by weight of methionine and 0.15 part by weight of lysine.

The In one embodiment, the composition may further comprise vitamin C.

In one embodiment of the present invention, The vitamin C contained in the composition may be contained in an amount of 0.02 part by weight based on the composition of the composition for broiler chicks.

In one embodiment of the present invention, the broiler feed is selected from the group consisting of corn, soybean meal, corn gluten, wheat flour, limestone, dicalcium phosphate, sodium chloride, methionine, lysine, trace mineral premix and vitamin premix ).

In one embodiment of the present invention, the broiler dietary composition comprises 47 to 48 parts by weight of corn, 25 to 35 parts by weight of soybean meal, 5 to 6 parts by weight of corn gluten, 9 to 10 parts by weight of wheat flour, 4 to 7 parts by weight of soybean oil 1 to 2 parts by weight of limestone, 1 to 2 parts by weight of dicalcium phosphate, 0.1 to 0.3 parts by weight of sodium chloride, 0.4 to 0.5 parts by weight of methionine, 0.4 to 0.5 parts by weight of lysine, 0.3 to 0.4 part by weight of trace mineral premix, 0.1 to 0.2 parts by weight of vitamin C, 0.01 to 0.02 parts by weight of vitamin C and 2 to 3 parts by weight of molasses.

Also, the present invention provides a method for producing a chicken broth comprising: (a) feeding hatching chicks to a broiler base stock for 21 days after hatching; And (b) feeding broiler diets supplemented with soybean milk, molasses, methionine, lysine and vitamin C to a basic diet for broilers from the 21 days after the lapse of 21 days, The present invention provides a broiler feeding method for reducing the heat stress of a broiler chicken, which is characterized by carrying out 12-hour lighting adjustment (female 08: 00-20: 00, name 20: 00-08: 00) in the environment.

In one embodiment of the present invention, in the step (b), the feeding of the compounded feed for broilers is performed from 18:00 to 8:00 in order to prevent the body temperature from further rising due to generation of metabolic heat in broilers .

In one embodiment of the present invention, the broiler base feed may be composed of corn, soybean meal, corn gluten, wheat flour, limestone, dicalcium phosphate, sodium chloride, methionine, lysine, trace minerals premix and vitamin premix.

In one embodiment of the present invention, 5 parts by weight of soybean oil, 2 parts by weight of molasses, 0.15 parts by weight of methionine, 0.15 parts by weight of lysine and 0.02 parts by weight of vitamin C,

In one embodiment of the present invention, the broiler feed includes 47 to 48 parts by weight of corn, 25 to 35 parts by weight of soybean meal, 5 to 6 parts by weight of corn gluten, 9 to 10 parts by weight of wheat flour, 4 to 7 parts by weight of soybean oil 1 to 2 parts by weight of limestone, 1 to 2 parts by weight of dicalcium phosphate, 0.1 to 0.3 parts by weight of sodium chloride, 0.4 to 0.5 parts by weight of methionine, 0.4 to 0.5 parts by weight of lysine, 0.3 to 0.4 part by weight of trace mineral premix, To 0.2 parts by weight of vitamin C, 0.01 to 0.02 parts by weight of vitamin C and 2 to 3 parts by weight of molasses.

The compound feed composition for broilers according to the present invention stimulates the proliferation of the immune system to increase the secretion amount of the blood immunity substance and not only reduce the stress hormone corticosterone but also maintain the proper acidity of the cecum, By promoting the growth of microorganisms and inhibiting the growth of harmful microorganisms, it is possible to effectively reduce the heat stress of broiler chickens through the action mechanism that improves the growth ability of broiler chickens. In addition, the method of breeding the broiler according to the present invention is a method of feeding the diets having different compositions according to the growth stage of the broiler, and in particular, in order to minimize the heat stress due to the 12 hour lighting control and the metabolic heat generation in the broiler, , The heat stress of broiler chickens can be effectively mitigated. Therefore, when the compounded feed and the breeding method of the present invention are used, it is possible to improve the productivity of broiler chickens by raising the resistance ability of the broiler chicken to heat stress (immune cell activation, reduction of stress hormone, maintenance of cecal microflora, etc.) You can contribute.

In one aspect, the present invention relates to a compounded feed composition for broiler chickens having a resistance to heat stress in broiler chickens, which further contains soybean oil, molasses, methionine, and lysine in a basic diet for broiler chickens.

In the present invention, "heat stress" refers to a change in the physiological change (blood parameter abnormality, increase in stress hormone, decrease in immunity, decrease in activity, gasp At the same time, increase in the amount of water, decrease in feed intake, and decrease in shipment weight). Thermal stress is synonymous with high temperature stress.

In the present invention, the 'resistance to heat stress effect' means an effect of alleviating or reducing the heat stress of the broiler which occurs under a high temperature environment.

The compounded feed composition for broilers according to the present invention has an effect of relieving or reducing the stress due to the high temperature of the broiler raised under high temperature (30 ° C or more) In other words, the compounded feed composition for broiler according to the present invention stimulates broiler immune organ cell proliferation to increase the secretion amount of blood immunity substance, and not only reduces the stress hormone corticosterone, but also maintains the proper acidity of the cecum, And to inhibit the growth of harmful microorganisms through the action mechanism can effectively reduce the heat stress of the broiler.

In the following Example 2 of the present invention, the content of immunoglobulin (IgG) in the blood was high in the experimental group (experimental group 9 or 10) fed with the broiler feed of the present invention under the conditions of the broiler chickens under the heat of the rain, The concentration of corticosterone was low. Especially, in the experimental group 10, higher immunity and lower concentration of stress hormone were detected compared to the chicken (group 6) that was raised under normal conditions without the heat wave condition. As a result of examining the microorganism changes in broiler cecum, it was confirmed that harmful microorganisms were significantly lower in the experimental group (experimental group 9 or experimental group 10) fed with the broiler feed of the present invention. It has been confirmed through experimentation that the compounded feed composition for broiler according to the present invention effectively reduces the heat stress of the broiler under the high temperature (30 ° C or more) temperature.

The compound feed composition for broilers according to the present invention is characterized in that soybean oil, molasses, methionine and lysine are further contained in the basic feed for broiler chickens.

The broiler basis feed used in the present invention may be composed of corn, soybean meal, corn gluten, wheat flour, limestone, dicalcium phosphate, sodium chloride, methionine, lysine, trace mineral premix and vitamin premix.

In one embodiment of the present invention, the compounded feed composition for broiler chickens according to the present invention may further contain soybean oil, molasses, methionine and lysine in a basic feed having the above-described composition, and soybean oil, molasses, The lysine may be 5 parts by weight of soybean oil, 2 parts by weight of molasses, 0.15 part by weight of methionine and 0.15 part by weight of lysine based on the compound composition for broiler chow.

In another embodiment of the present invention, the compounded feed composition for broiler chickens according to the present invention may further comprise vitamin C together with soybean oil, molasses, methionine and lysine in a basic diet for broiler chickens. The additional vitamin C is preferably 0.02 part by weight based on the compound composition for broiler chow.

In another embodiment of the present invention, the compounded feed composition for broiler chickens comprises 47 to 48 parts by weight of corn, 25 to 35 parts by weight of soybean meal, 5 to 6 parts by weight of corn gluten, 9 to 10 parts by weight of wheat flour, 1 to 2 parts by weight of limestone, 1 to 2 parts by weight of dicalcium phosphate, 0.1 to 0.3 parts by weight of sodium chloride, 0.4 to 0.5 parts by weight of methionine, 0.4 to 0.5 parts by weight of lysine, 0.3 to 0.4 parts by weight of trace mineral premix, 0.1 to 0.2 parts by weight of vitamin premix, 0.01 to 0.02 parts by weight of vitamin C and 2 to 3 parts by weight of molasses.

In another aspect, the present invention provides a method for producing broiler chickens, comprising the steps of: (a) feeding hatching chickens in a broiler base stock for 21 days after hatching; And (b) feeding broiler diets supplemented with soybean milk, molasses, methionine, lysine and vitamin C to a basic diet for broilers from the 21 days after the lapse of 21 days, The present invention relates to a method of breeding broiler chickens that reduces heat stress in broiler chicks, which is characterized by performing 12-hour lighting control (female 8: 00-20: 00, 20: 00-08: 00) in the environment.

In the step (b), it is preferable to feed the compounded feed for broiler chickens at the time of 18: 00-8: 00 in order to prevent the body temperature from further rising due to the generation of metabolic heat in broiler chickens.

The broiler base feed may be composed of corn, soybean meal, corn gluten, wheat flour, limestone, dicalcium phosphate, sodium chloride, methionine, lysine, trace minerals premix and vitamin premix.

5 parts by weight of soybean oil, 2 parts by weight of molasses, 0.15 part by weight of methionine, 0.15 part by weight of lysine and 0.15 part by weight of vitamin C were added to soybean milk, molasses, methionine, lysine and vitamin C, 0.02 parts by weight .

In one embodiment of the present invention, the broiler feed includes 47 to 48 parts by weight of corn, 25 to 35 parts by weight of soybean meal, 5 to 6 parts by weight of corn gluten, 9 to 10 parts by weight of wheat flour, 4 to 7 parts by weight of soybean oil, 1 to 2 parts by weight of limestone, 1 to 2 parts by weight of dicalcium phosphate, 0.1 to 0.3 parts by weight of sodium chloride, 0.4 to 0.5 parts by weight of methionine, 0.4 to 0.5 parts by weight of lysine, 0.3 to 0.4 parts by weight of trace minerals premix, , 0.01 to 0.02 parts by weight of vitamin C and 2 to 3 parts by weight of molasses.

The broiler chickens according to the present invention are fed through feedstuffs having different compositions according to the growth stage of the broiler chickens. In particular, in order to minimize the heat stress due to the 12 hours lighting control and the metabolic heat generation in the broiler chickens, Through the feeding method, the heat stress of the broiler can be effectively alleviated.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.

< Example  1>

Broiler feed Heat stress  analysis

In order to test the degree of thermal stress relief of the broiler according to the composition of the compounded feed, the present inventors firstly applied the mixed feed having the composition ratio shown in Table 1 below to the broiler chickens and then applied heat stress for a certain period of time, We analyzed the changes of material, immune organ weights and microorganisms in the cecum.

All laboratory procedures, including animals, have been subject to the scientific and ethical rules set forth in the European Experimental Animal Handbook, and have been approved by the Animal Experimental Ethics Committee of Kangwon National University. 375 chicks of Ross system were supplied from Hanyang hatchery on the day of hatching, and the productivity was examined by feeding the compounded feed having the composition ratio shown in Table 1 for 32 days after hatching. At this time, 3 repetitions of 5 treatments (25 pens per 1.65 m ^{2 of} repetitive pen) were completely randomized. During the whole experimental period, water and compound feed were freely consumed. From the 28th day to the 32th day after hatching, 5 hours (11: 00-16: 00hrs) was given to the experimental group 2 to the experimental group 5 except the experimental group 1 (normal condition) (33 ± 1 ° C) and maintained at a relative humidity of 70% for 24 hours. From the start of the test to the 21st day, all experimental groups fed the same electro diet (see Table 1 below: basal diet + Uji).

Broiler feed 1 Ingredients
(% as-fed) Growth stage Electricity
(0-21 days) Experiment 1
review
(22-35 days) Experiment 2
review
(22-35 days) Experiment group 3
review
(22-35 days) Experiment group 4
review
(22-35 days) Experiment group 5
review
(22-35 days) Basic feed corn 52.00 50.00 50.00 45 44.70 44.67 Soybean meal 34.00 25.00 25.00 25.00 25.00 25.00 Corn gluten 4.70 5.70 5.70 5.70 5.70 5.70 flour - 10.00 10.00 10.00 10.00 10.00 limestone 1.25 1.25 1.25 1.25 1.25 1.25 Calcium phosphate 1.70 1.70 1.70 1.70 1.70 1.70 Sodium chloride 0.25 0.25 0.25 0.25 0.25 0.25 Methionine 0.30 0.30 0.30 0.30 0.30 0.30 Lysine 0.30 0.30 0.30 0.30 0.30 0.30 mineral
Premix 0.34 0.34 0.34 0.34 0.34 0.34 vitamin
Premix 0.16 0.16 0.16 0.16 0.16 0.16 Compounding material Uji 5.00 5.00 5.00 - - - Soybean oil - - - 5.00 5.00 5.00 molasses - - - 5.00 5.00 5.00 Welcome - - - - 0.15 0.15 Lysine - - - - 0.15 0.15 Vitamin C - - - - - 0.03
(300 ppm) sum 100 100 100 100 100 100 Heat stress treatment none none has exist has exist has exist has exist

Methionine: DL-Met, 50%, Lysine: L-Lys HCl, 78%, mineral premix: trace mineral premix

As a result, the body weight gain, feed intake and blood immunity were significantly higher in the experimental group 1 treated with the normal condition than the other experimental groups, and the experimental group 2, the experimental group 3, the experimental group 4 and the experimental group 5, No statistically significant differences between the two groups were observed (see Tables 2 and 3 below). The numbers of sacrifice of broiler chickens due to heat stress were higher in the order of experimental group 5, experimental group 2, experimental group 3, and experimental group 4, but no sacrifice occurred in experimental group 1 (see Table 2 below). The weight of the thymus, spleen, and F sac of the immunized organs was the highest in the experimental group 1, and the experimental group 2 and the experimental group 3 were higher than the experimental group 4 and the experimental group 5, respectively, and there was a statistically significant difference (see Table 2 below). Among the cecal microorganisms, Lactobacillus was the highest in the experimental group 1, and the highest in the experimental group 2, the experimental group 3, the experimental group 4, and the experimental group 5, except for the difference between the experimental group 4 and the experimental group 5, The car was recognized. Total aerobic bacteria, Escherichia coli and Salmonella, which were harmful bacteria, showed the lowest value in the experimental group 1 and statistically significant difference was shown between the treatments (see Table 4 below).

From the above results, it can be seen that the feeding of the broiler diets of Table 1 does not show a heat stress reducing effect, presumably because the level of the additives added in the compound feed at the same time as the continuous illumination (24 hours) (In our experiment group 5, our company was the most abundant). Chickens have a characteristic of consuming feed all day if they have a bright fire, and if they eat food when exposed to heat, the body temperature may increase due to the metabolism heat, which may increase the heat stress damage.

Growth ability of broiler (g / head) Evaluation items Experimental group Experiment 1 Experiment 2 Experiment group 3 Experiment group 4 Experiment group 5 pooled SEM Weight gain 1,581 1,471 1,401 1,257 1,235 18.770 Feed intake 2,624 2,404 2,228 2,010 2,064 24.571 Feed efficiency 0.60 0.61 0.62 0.62 0.60 0.0022 Our company 0 4 3 2 6 -

Blood immunity and immunogen weight (organ weight / fresh weight,%) Evaluation items Experimental group Experiment 1 Experiment 2 Experiment group 3 Experiment group 4 Experiment group 5 pooled SEM IgG (μg / ml) 174.81 102.58 84.01 77.57 77.85 0.7019 F Bag (Bursa) Weight (%) 0.23 0.20 0.19 0.11 0.14 0.0081 Spleen weight (%) 0.17 0.15 0.12 0.08 0.09 0.0020 Thymus weight (%) 0.22 0.18 0.19 0.14 0.12 0.0095

Changes in cecal microorganisms (Log10 cfu / cecum content g) Evaluation items Experimental group Experiment 1 Experiment 2 Experiment group 3 Experiment group 4 Experiment group 5 pooled SEM Lactobacillus 8.07 7.85 7.26 6.01 4.78 0.1457 Total aerobic bacteria (%) 5.16 5.51 5.20 8.41 8.87 0.0278 Escherichia coli (%) 3.20 3.35 4.61 6.68 6.50 0.1651 Salmonella (%) 2.72 3.19 3.08 4.07 4.41 0.1218

< Example  2>

Broiler Heat stress Reduce (mitigate)  Analysis of the best combination of feed and breeding methods available

In Example 1, the fact that the continuous lighting can increase the body temperature due to the metabolic heat generation due to excessive consumption of the feed to increase the heat stress in breeding the broiler, and the fact that inappropriate ingredients (soybean oil, molasses, Methionine, lysine, Vitamin C) did not contribute to the improvement of growth performance and immune function of broiler chicks. Based on the above results, the optimal composition ratio of feedstuffs to reduce (alleviate) heat stress in broiler chickens was analyzed (8: 00-20: 00, 20: 00-08: 00) under the conditions of heat and light for 12 hours, and the feeding time was changed from 18:00 to 8: 00.

For the experiment, 360 Ross broiler chicks were fed from hatchery hatchery on the day of hatching, and the productivity of broiler chickens was investigated by feeding the compound feed of Table 5 below. At this time, 3 repetitions of 6 treatments (20 pens per 1.65 m ^{2 of} repetitive pen) were completely randomized. From the start of the test to the 21st day, all experimental groups were fed with the same amount of feed (see Table 1 below: base feed + Uji). Until 22-27 days, Illumination was performed. The relative humidity was maintained at 70% with the high temperature (33 ± 1 ° C) condition of 5 hours (11: 00-16: 00) daily from day 28 to day 32 and lighting control , 20: 00-08: 00). (18: 00-24: 00 repeatedly 1.5 kg per pen manual feeding + 24: 00-08: 00) under high temperature conditions artificially imposed for 5 days from 28 to 32 days Repeated 1.5 kg per pen). The temperature was maintained at 25 ° C as soon as the heat stress was completed during the day.

Broiler feed 2 Ingredients
(% as-fed) Growth stage Electricity
(0-21 days) Experiment group 6
review
(22-35 days) Experiment group 7
review
(22-35) Experiment group 8
review
(22-35) Experiment group 9
review
(22-35) Experiment group 10
review
(22-35) Experiment group 11
review
(22-35) Basic feed corn 52.00 50.00 50.00 45 47.70 47.68 47.67 Soybean meal 34.00 25.00 25.00 25.00 25.00 25.00 25.00 Corn gluten 4.70 5.70 5.70 5.70 5.70 5.70 5.70 flour - 10.00 10.00 10.00 10.00 10.00 10.00 limestone 1.25 1.25 1.25 1.25 1.25 1.25 1.25 Calcium phosphate 1.70 1.70 1.70 1.70 1.70 1.70 1.70 Sodium chloride 0.25 0.25 0.25 0.25 0.25 0.25 0.25 Methionine 0.30 0.30 0.30 0.30 0.30 0.30 0.30 Lysine 0.30 0.30 0.30 0.30 0.30 0.30 0.30 mineral
Premix 0.34 0.34 0.34 0.34 0.34 0.34 0.34 vitamin
Premix 0.16 0.16 0.16 0.16 0.16 0.16 0.16 Compounding material Uji 5.00 5.00 5.00 - - - - Soybean oil - - - 5.00 5.00 5.00 5.00 molasses - - - 5.00 2.00 2.00 2.00 Welcome - - - - 0.15 0.15 0.15 Lysine - - - - 0.15 0.15 0.15 Vitamin C - - - - - 0.02
(200 ppm) 0.03
(300 ppm) sum 100 100 100 100 100 100 100 Heat stress treatment none none has exist has exist has exist has exist has exist

Methionine: DL-Met, 50%, Lysine: L-Lys HCl, 78%, mineral premix: trace mineral premix

As a result, for 21 days from the start of the experiment There was no statistically significant difference between broiler chicks fed the electro diet (basal diet + woji) under normal conditions. The daily weight gain and daily dietary intake of broiler chicks fed unlimited feeds of Table 5 above under normal conditions from 22 to 27 days before induction of heat stress (heat wave condition) were significantly higher in experimental group 8 and 9 than in the other experimental groups Respectively. On the other hand, the body weight gain was significantly higher in the experimental group 10 than the experimental groups 6 and 7 one week before the induction of heat stress (heat wave condition), but the statistical significance was not recognized between the experimental groups 6 and 7. The daily food intake was 6, , And no statistically significant difference was observed between the experimental group 10. From the 28th to the 32nd of the day, the heat stress was induced by artificially applying the heat of 33 ℃ for 5 hours. The body weight gain of the broiler was the highest in the experimental group 10, followed by the experimental group 6, the experimental group 7, and the experimental group 9 , Experimental group 11, and experimental group 8. The total body weight gain was significantly higher in the experimental groups 10 and 9 than in the experimental group 10, the experimental group 9, and the experimental group 6, 7, 8, and 11, while the total feed intake was not significantly different (See Table 6 below). &Lt; tb &gt;&lt; TABLE &gt;

In addition, the weight of the immunological organs in the living tissue was the lowest in the experimental group 8 in the F sac, the thymus, and the spleen, and the highest in the experimental group 10 and statistically significant difference was observed between the treatment groups (see Table 7 below).

Immunoglobulin (IgG) in blood was higher in the order of experimental group 10, experimental group 6, experimental group 9, experimental group 11, experimental group 8, and experimental group 7, and corticosterone, which is a stress hormone in blood, 11, 9, 6, and 10, respectively (see Table 8 below). The results showed that the experimental group 10 had the highest level of immune substances in blood and the lowest level of stress hormone. Also in the case of Experiment group 9, the level of immunological substance in blood was good and the level of the stress hormone corticosterone was also relatively low.

Among the harmful microorganisms in the feces, total aerobic bacteria were higher in experimental group 8 and 11 than in experimental group 8, experimental group 11, experimental group 6, experimental group 7, experimental group 9, and experimental group 10. E. coli was higher in the experimental group 11, 6, experimental group 8, experimental group 7, experimental group 9, and experimental group 10 (see Table 9 below). It was found that the cecum environment was the best among the experimental groups other than experimental group 6 raised under normal conditions.

The total volatile fatty acids measured in the contents of the cecum were significantly higher in the experimental group 9 and the experimental group 10 than in the experimental group 11, the experimental group 6, the experimental group 7, and the experimental group 8 (see Table 10 below). The content of acetic acid and propionic acid was the highest among the volatile fatty acids, and the increase of volatile fatty acid could contribute to increase the acidity of the cecum.

Growth ability of broiler (g / head) Evaluation items Experimental group 6 7 8 9 10 11 Pooled
SEM Weight gain
(0-21 days) 1,064 1,059 1,058 1,060 1,066 1,059 20.215 Daily weight gain
(22-27 days) 64.83 63.00 82.00 79.66 68.01 63.00 0.8902 Daily weight gain
(28-32 days) 41.37 37.44 23.25 34.02 50.41 34.00 1.5071 Total weight gain
(0-32 days) 1,658 1,624 1,666 1,708 1,726 1,607 22.081 Feed intake
(0-21 days) 1,624 1,620 1,638 1,631 1,624 1,620 27.025 Daily feed intake
(22-27 days) 93.33 96.83 127.2 127.7 97.71 96.00 18.155 Daily feed intake
(28-32 days) 105.4 96.04 76.32 75.80 110.5 98.80 3.7711 Total feed intake
(0-32 days) 2,707 2,681 2,781 2,772 2,760 2,690 21.098 Feed efficiency 0.61 0.61 0.60 0.62 0.63 0.61 0.0052 Our company (%) One 0 0 0 0 0 -

Immune organ weight (organ weight / fresh weight,%) Evaluation items Experimental group 6 7 8 9 10 11 Pooled
SEM F Bag (Bursa) Weight (%) 0.23 0.22 0.18 0.21 0.24 0.20 0.0057 Spleen weight (%) 0.17 0.18 0.15 0.18 0.19 17 0.0032 Thymus weight (%) 0.20 0.21 0.17 0.18 0.22 18 0.0069

Blood immunity and corticosterone concentration (μg / mL) Evaluation items Experimental group 6 7 8 9 10 11 Pooled
SEM IgG 241.7 97.02 101.5 222.1 269.7 120.5 0.5133 corticosterone 10.04 67.19 75.61 15.01 7.33 67.20 0.1205

Changes in cecal microorganisms (Log10 cfu / cecum content g) Evaluation items Experimental group 6 7 8 9 10 11 Pooled
SEM Total aerobic bacteria 7.81 8.12 9.06 7.68 7.37 9.12 0.0831 coliform 7.63 7.60 8.57 7.07 6.73 8.21 0.1215 E. coli 3.83 3.13 3.73 3.16 3.09 4.02 0.1377

Volatile fatty acids in the cecum (μmol / contents of the cecum g) Evaluation items Experimental group 6 7 8 9 10 11 Pooled
SEM Acetic acid 100.77 87.01 86.43 128.19 127.03 110.23 8.1760 Propionic acid 33.09 24.34 22.03 36.51 35.81 33.00 0.5380 Butyrate 10.51 13.74 15.87 5.12 4.89 11.21 0.7489 Isobutyric acid 6.50 8.09 7.88 4.67 4.71 4.03 0.5801 Valeric acid 5.52 5.66 5.93 3.09 1.55 1.92 0.1255 Isovaleric acid 3.07 3.26 3.70 1.17 1.25 3.07 0.3821 Total SCFA 159.4 142.1 141.84 178.7 175.2 163.46 7.1885

In conclusion, feeding the experimental group 9 or the experimental group 10 to the broiler under heat stress of 33 ° C stimulates the immune system cell proliferation to increase the secretion amount of the blood immunity substance and reduce the stress hormone corticosterone, And the growth rate of the broiler chickens was improved by inhibiting the growth of harmful microorganisms. Therefore, it was concluded that the experimental group 9 or the experimental group 10 was most suitable for the broiler feed having the optimum mixing ratio having the resistance effect against the heat stress of the broiler.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (11)

delete delete delete delete delete delete (a) feeding hatching chickens in a broiler base feed with a combination of tallow, from a hatching day to a 21st day; And
(b) feeding the broiler dietary supplement containing the soybean oil, molasses, methionine, lysine and vitamin C to the basic diet for broilers from the time when 21 days have elapsed, and in a high temperature environment exceeding 30 캜 A method of brooding a broiler which reduces heat stress in broiler chickens, characterized in that the broiler is lighted from 8 o'clock to 20 o'clock and lighted from 20 o'clock to 8 o'clock for 12 hours. 8. The method of claim 7,
In the step (b), the feeding of the compounded feed for broilers is performed from 18:00 to 8:00 in order to prevent the body temperature from rising due to generation of metabolic heat in the broiler. How to breed a broiler. 8. The method of claim 7,
Characterized in that the broiler base feed comprises corn, soybean meal, corn gluten, wheat flour, limestone, dicalcium phosphate, sodium chloride, methionine, lysine, trace mineral premix and vitamin premix. A method of brooding a broiler which reduces heat stress. 8. The method of claim 7,
5 parts by weight of soybean oil, 2 parts by weight of molasses, 0.15 part by weight of methionine, 0.15 part by weight of lysine and 0.02 part by weight of vitamin C were added to the broiler base stock, based on the compounding feed for broiler chicks supplemented with soybean oil, molasses, methionine and lysine Wherein the heat stress of the broiler is reduced. 8. The method of claim 7,
The broiler chowder mixture contains 47 to 48 parts by weight of corn, 25 to 35 parts by weight of corn gluten, 5 to 6 parts by weight of corn gluten, 9 to 10 parts by weight of wheat flour, 4 to 7 parts by weight of soybean oil, 1 to 2 parts by weight of limestone, 1 to 2 parts by weight of calcium, 0.1 to 0.3 parts by weight of sodium chloride, 0.4 to 0.5 parts by weight of methionine, 0.4 to 0.5 parts by weight of lysine, 0.3 to 0.4 parts by weight of trace minerals premix and 0.1 to 0.2 parts by weight of vitamin premix, 0.02 part by weight of molasses and 2 to 3 parts by weight of molasses.