KR101386075B1 - Biomarkers for identification of exposure to Hexachlorobenzene and the method of identification using thereof - Google Patents
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Abstract
본 발명은 헥사클로로벤젠(Hexachlorobenzene)에 대한 노출 여부 확인용 바이오마커 및 이를 이용한 확인 방법에 관한 것으로, 구체적으로 잔류성 유기 오염물 중 하나인 헥사클로로벤젠에 의해 대조군에 비해 특이적으로 유전자 발현이 증가 또는 감소하는 바이오마커 및 이를 이용한 헥사클로로벤젠에 대한 노출 여부를 확인하는 방법에 관한 것이다. 본 발명의 바이오마커가 집적된 DNA 마이크로어레이 칩은 인간의 간에 대한 헥사클로로벤젠 노출을 판정하는데 유용하게 사용될 수 있으며, 헥사클로로벤젠에 의해 유발되는 독성 작용 기작을 규명하는 도구로 이용될 수 있다.The present invention relates to a biomarker for confirming exposure to hexachlorobenzene and a method for identifying the same using hexachlorobenzene, and specifically, gene expression is increased or increased compared to the control group by hexachlorobenzene, which is one of residual organic contaminants. It relates to a decreasing biomarker and a method for identifying exposure to hexachlorobenzene using the same. The DNA microarray chip incorporating the biomarker of the present invention can be usefully used to determine hexachlorobenzene exposure to the human liver, and can be used as a tool for identifying the toxic mechanism of action induced by hexachlorobenzene.
헥사클로로벤젠, 잔류성 유기 오염물, 살충제, 바이오마커, DNA 마이크로어레이 칩. Hexachlorobenzene, persistent organic contaminants, pesticides, biomarkers, DNA microarray chips.
Description
본 발명은 헥사클로로벤젠(Hexachlorobenzene)에 대한 노출 여부 확인용 바이오마커 및 이를 이용한 확인 방법에 관한 것으로, 더욱 상세하게는 잔류성 유기 오염물 중 하나인 헥사클로로벤젠에 의해 대조군에 비해 특이적으로 유전자 발현이 증가 또는 감소하는 바이오마커 및 이를 이용한 헥사클로로벤젠에 대한 노출 여부를 확인하는 방법에 관한 것이다.The present invention relates to a biomarker for confirming exposure to hexachlorobenzene and a method of identifying the same, and more specifically, to hexachlorobenzene, which is one of residual organic contaminants, gene expression is specifically compared to a control group. It relates to an increasing or decreasing biomarker and a method for identifying the exposure to hexachlorobenzene using the same.
잔류성 유기오염물의 하나인 헥사클로로벤젠(Hexachlorobenzene)은 주로 염소계수소화합물의 생산과정에서 폐산물로서 형성되며 몇가지 농약의 오염물질로 존재하여 대기방출이나 폐기물소각에 의한 비산재에 의해 환경중으로 방출된다. HCB는 화학적으로 안정하고 미생물에 의한 분해가 적어 잔류성이 크다. 대기, 오염된 음용수나 음식물섭취, 토양과의 접촉 또는 작업환경에 의해 주로 노출된다. 잔류 성 유기오염물은 수천 마일의 이동성과 먹이 사슬에 따라 생체에 축적되고, 환경에 지속적으로 존재하여 식물, 동물, 인간에 위해를 유발하는 화학물질이다(James CY et al., Ecotoxicol. environ. saf, 72:162-172, 2009). 헥사클로로벤젠은 신경계 손상, 암 유발, 내분비계 장애 및 인체의 전체적인 기관계에 독성을 나타내는 것으로 보고된 바 있다(ASTDR 1995). 우리나라에서는 도입이 되지 않았다. 대기 중에 방출되면 증기와 고체상에 분배되며 직접적인 광분해에 의한 반감기는 약 80일(Mill and Haag, 1986), 간접적 광분해인 광산화에 의한 반감기는 156.4일~4.2년(Howard et al., 1991; Atkinson, 1987)으로 추정된다. 대기 중에서의 HCB 반감기는 2~6년(Mackay et al.,1992)이라고 보고된 바도 있다. 대기 중에서 강우나 침강에 의해 제거된다. 장거리이동이 가능하여 북극의 대기, 눈, 해수, 식물 및 생물체에서 검출된다(Parrish et al., 1974; Veith et al., 1979; Oehme and Stray, 1982; Oliver 문 Niimi, 1983; Oliver, 1987; Hargrave et al., 1988;1989; Norstrom et al., 1990).Hexachlorobenzene, one of the persistent organic pollutants, is mainly formed as a waste product during the production of chlorine-hydrogen compounds and exists as pollutants of several pesticides and is released into the environment by air ash or fly ash from waste incineration. HCB is chemically stable and has low residuals due to less degradation by microorganisms. Mainly exposed to air, contaminated drinking water or food intake, contact with soil or working environment. Persistent organic contaminants are chemicals that accumulate in living organisms along thousands of miles of mobility and food chains, and are persistent in the environment, causing harm to plants, animals, and humans (James CY et al. , Ecotoxicol. Environ. Saf , 72: 162-172, 2009). Hexachlorobenzene has been reported to be toxic to nervous system damage, cancer causing, endocrine disorders, and the entire organ system of the human body (ASTDR 1995). It was not introduced in Korea. When released into the atmosphere, it is distributed in vapor and solid phases, and the half-life by direct photolysis is about 80 days (Mill and Haag, 1986), and the half-life by photo-oxidation, indirect photolysis, is 156.4-4.2 years (Howard et al., 1991; Atkinson, 1987). HCB half-life in the atmosphere has been reported to be 2-6 years (Mackay et al., 1992). It is removed from the atmosphere by rainfall or sedimentation. It is possible to travel long distances and is detected in the Arctic atmosphere, snow, seawater, plants and organisms (Parrish et al., 1974; Veith et al., 1979; Oehme and Stray, 1982; Oliver Moon Niimi, 1983; Oliver, 1987; Hargrave et al., 1988; 1989; Norstrom et al., 1990).
헥사클로로벤젠의 민물과 해양 생물체에 대한 생물농축계수는 민물조류(chlorella fusca)에 대해 24,800(Freitag et al., 1989), worm(Lumbriculus variegatus)106,804(Nebeker et al., 1989), grass shrimp 11,458, sheephead minnow 6,692, pinfish 21,000(Parrish et al.,1974)이었다. 몇몇 연구에서는 자연수생태계에서 먹이사슬의 상위에 위치하는 생물체에 축적된 HCB는 HCB의 화학적 성질에서 예측되는 수준 이상임을 보여주었다(Oliver, 1987; Oliver and Niimi, 1988). 또한 물고기를 먹는 조류(예를 들면, Herrin gull eggs, 1991, Great Lakes) 및 육식성 조류종(Noble and Elliott, 1986; Pearce et al.,1989; Noble et al., 1992)에서도 HCB가 검출되었다. 특히 송골매(Peregrine falcon)의 알에서 1,060 ng/g wet 이상의 농도로 검출됨으로써 HCB가 먹이사슬을 통해 생물농축됨을 알 수 있다.The bioconcentration coefficients for freshwater and marine organisms of hexachlorobenzene are 24,800 (Freitag et al., 1989), worm (Lumbriculus variegatus) 106,804 (Nebeker et al., 1989), grass shrimp 11,458 for freshwater algae (chlorella fusca) . sheephead minnow 6,692 and pinfish 21,000 (Parrish et al., 1974). Some studies have shown that HCB accumulated in organisms at the top of the food chain in the natural aquatic ecosystem is above levels predicted by the chemical properties of HCB (Oliver, 1987; Oliver and Niimi, 1988). HCB has also been detected in fish-eating birds (eg Herrin gull eggs, 1991, Great Lakes) and carnivorous algae species (Noble and Elliott, 1986; Pearce et al., 1989; Noble et al., 1992). In particular, peregrine falcon eggs were detected at concentrations of 1,060 ng / g or more, indicating that HCB is bioconcentrated through the food chain.
헥사클로로벤젠의 주로 인체의 유방암 세포 및 조직과 생체 시료의 모니터링 관련된 연구에 거의 국한되어 있다. 그리고 상기에서 언급한 것과 같이 헥사클로로벤젠의 인간 간에 대해 장애를 일으킬 가능성에도 인체에서의 위해도 평가 데이터가 충분하지 않고, 노출에 대한 검색 방법 역시 기체크로마토그래피(Gas Chromatography-Mass Spectrometer: GC-MS) 또는 고성능액체크로마토그래피(High Performance Liquid Chromatography: HPLC) 등의 고전적인 방법에 국한되어 있다. 상기 GC-MS 또는 HPLC 방법 등을 이용할 경우 정량적 분석이 가능하나 분석을 위한 적정 조건을 설정하여야 하며 고가의 장비 등이 필요하다. 그러므로 더욱 빠르고 간편한 스크리닝 방법, 예를 들면 프라이머(primer)를 이용하는 실시간(real-time) RT-PCR(reverse transcription polymerase chain reaction) 또는 DNA 마이크로어레이 칩 등을 이용하여 신속한 위해성 평가를 통해 인체에서의 독성작용을 탐색할 수 있는 분자적 지표를 발굴하고 활용하여 헥사클로로벤젠의 노출에 대한 적절한 대책 및 관리를 수행하는 것이 중요한 과제라 하겠다.Hexachlorobenzene is largely limited to studies related to the monitoring of human breast cancer cells and tissues and biological samples. As mentioned above, there is not enough data on risk assessment in humans and the possibility of hexachlorobenzene in human liver, and the detection method for exposure is also based on gas chromatography (GC-MS). ) Or classical methods such as High Performance Liquid Chromatography (HPLC). When using the GC-MS or HPLC method, quantitative analysis is possible, but appropriate conditions for analysis must be set, and expensive equipment is required. Therefore, faster and simpler screening methods, such as real-time reverse transcription polymerase chain reaction (RT-PCR) or DNA microarray chips using primers, can be used for rapid toxicity assessment in humans. It is an important task to identify and utilize the molecular indicators that can explore the action and to take appropriate measures and control against the exposure of hexachlorobenzene.
포유류 6종, 미생물 292종 등 여러 종의 게놈(genome) 염기서열 프로젝트가 완성되어 NCBI(National Center for Biotechnology Information)에 보고되었다. 이렇게 얻어진 막대한 양의 데이터를 기본으로 유전자의 기능을 연구하기 위하여 게놈-와이드 익스프레션(genome-wide expression) 연구가 이루어지고 있다. 즉, 한 번의 실험으로 수천 개의 유전자의 발현을 분석하기 위하여 DNA 마이크로어레이(microarray) 분석을 수행했다(Schena M et al., Proc. Natl. Acad. Sci. USA 93:10614-10619, 1996). The genome sequencing project of several species, including 6 mammals and 292 microbes, has been completed and reported in the National Center for Biotechnology Information (NCBI). Genome-wide expression studies have been conducted to study the function of genes based on the vast amount of data thus obtained. That is, DNA microarray analysis was performed to analyze the expression of thousands of genes in one experiment (Schena M et al., Proc. Natl. Acad. Sci. USA 93: 10614-10619, 1996).
마이크로어레이는 cDNA(complementary DNA)나 20 - 25 염기쌍(base pair) 길이의 올리고뉴클레오티드(oligonucleotide)들의 세트를 유리에 집적화한 것이다. cDNA 마이크로어레이는 학교 내의 연구실 또는 Agilent, Genomic Solutions 등의 회사에서 칩 위에 cDNA 수집물을 기계적으로 고정화하거나 잉크젯(ink jetting) 방법을 이용하여 생산하고 있다(Sellheyer K et al., J. Am. Acad. Dermatol. 51:681-692, 2004). 올리고뉴클레오티드 마이크로어레이는 Affymetrix사에서 사진 식각 공정(photolithography)을 이용하여 칩 위에서 직접 합성하는 방법에 의해 만들어지고 있으며, Agillent사 등에는 합성된 올리고뉴클레오티드를 고정화하는 방법으로 생산하고 있다(Sellheyer K et al., J. Am. Acad. Dermatol. 51:681-692, 2004).A microarray is a collection of cDNA (complementary DNA) or a set of oligonucleotides of 20-25 base pairs in length on glass. cDNA microarrays are produced by labs in schools or companies such as Agilent, Genomic Solutions, etc., by mechanically immobilizing cDNA collections on chips or by using ink jetting (Sellheyer K et al., J. Am. Acad). Dermatol 51: 681-692, 2004). Oligonucleotide microarrays are made by Affymetrix by direct synthesis on a chip using photolithography, and by Agillent et al ., Synthesized oligonucleotides are immobilized (Sellheyer K et al. , J. Am. Acad. Dermatol . 51: 681-692, 2004).
유전자 발현의 분석을 위해서는 조직 등 시료에서 RNA를 수득하여 형광이나 동위원소로 표지화하며 cDNA로 전환시킨 후, DNA 마이크로어레이에 있는 올리고뉴클레오티드와 혼성화 반응을 수행한다. 올리고마이크로어레이는 주로 두 개의 다른 형광(예: Cy3 및 Cy5)으로 대조군과 실험군을 각각 표지화하여 같은 칩 상에서 동시에 혼성화 반응을 수행한 후 광학적으로 이미지를 스캔하여 형광의 세기를 얻 고 그 결과를 분석한다. 두 개의 형광 세기의 비율에 따라 유전자의 발현 여부를 결정한다(Somasundaram K et al., Genomics Proteomics I:1-10, 2002). 최근 DNA 마크로어레이 기술을 이용한 첨단 기법인 독성 유전체학(Toxicogenomics) 연구 등과 접목하여 대량(high throughput)으로 의약품 및 신의약 후보물질은 물론 대표적인 환경오염물질을 비롯한 모든 화학물질에 의한 특정 조직이나 세포주에서 발현되는 유전자들의 발현 패턴의 분석 및 양적 분석이 가능해졌다. 이에 따라 특정 세포 내에서 특정 유전자의 발현 빈도를 분석함으로써 약물의 부작용 및 환경오염물질의 유해작용과 관련된 유전자의 발굴이 가능하며, 이를 통하여 환경오염물질의 유해작용과 약물의 작용 및 부작용에 따른 분자적 메커니즘을 이해하게 될 것이며 나아가 독성 및 부작용을 유발하는 물질의 검색이 가능하게 될 것이다.For analysis of gene expression, RNA is obtained from a sample such as tissue, labeled with fluorescence or isotope, converted to cDNA, and hybridized with oligonucleotides in a DNA microarray. Oligomicroarrays mainly label the control and experimental groups with two different fluorescences (eg Cy3 and Cy5), perform hybridization on the same chip at the same time, and then optically scan the image to obtain fluorescence intensity and analyze the results. do. Gene expression is determined according to the ratio of the two fluorescence intensities (Somasundaram K et al., Genomics Proteomics I: 1-10, 2002). In combination with the recent research on toxic genomics (Toxicogenomics), which is an advanced technique using DNA macroarray technology, high-throughput expression in specific tissues or cell lines by all chemicals including drug and new drug candidates, as well as representative environmental pollutants Analysis and quantitative analysis of the expression patterns of genes are now possible. Accordingly, by analyzing the frequency of expression of specific genes in specific cells, it is possible to discover genes related to adverse effects of drugs and harmful effects of environmental pollutants, and through this, molecules according to the harmful effects of environmental pollutants, drugs, and side effects. It will be possible to understand the mechanisms and further to search for substances that cause toxicity and side effects.
이에, 본 발명자들은 인간 유전자 45,220개의 단편이 집적된 올리고마이크로어레이를 이용하여 헥사클로로벤젠에 노출된 인간 간암 세포인 HepG2 세포주에서의 유전자 발현 프로파일을 관찰 및 분석함으로써, 헥사클로로벤젠에 의해 대조군에 비해 특이적으로 유전자 발현이 증가 또는 감소하는 유전자를 발굴하여, 헥사클로로벤젠 노출 여부를 확인할 수 있는 바이오마커 및 이를 이용한 노출 여부를 확인하는 방법을 확립하여 본 발명을 완성하였다.Therefore, the present inventors observed and analyzed the gene expression profile in HepG2 cell line, which is human liver cancer cells exposed to hexachlorobenzene, using oligomicroarray in which 45,220 fragments of human genes were accumulated. The present invention has been completed by establishing a biomarker capable of confirming exposure to hexachlorobenzene and a method of identifying exposure using the same by discovering a gene that specifically increases or decreases gene expression.
본 발명의 목적은 헥사클로로벤젠(Hexachlorobenzene) 노출에 의해 과발현 또는 저발현되는 바이오마커 및 상기 바이오마커를 이용한 헥사클로로벤젠에 대한 노출 여부를 확인하는 방법을 제공하는 것이다.An object of the present invention is to provide a biomarker that is overexpressed or underexpressed by hexachlorobenzene exposure and a method for confirming exposure to hexachlorobenzene using the biomarker.
상기 목적을 달성하기 위하여, 본 발명은 잔류성 유기 오염질인 헥사클로로벤젠(Hexachlorobenzene) 노출에 의해 발현 변화를 일으키는 것을 특징으로 하는 헥사클로로벤젠에 대한 노출 여부 확인용 바이오마커를 제공한다.In order to achieve the above object, the present invention provides a biomarker for confirming the exposure to hexachlorobenzene, characterized in that the expression changes by the exposure of the residual organic hexachlorobenzene (Hexachlorobenzene).
또한, 본 발명은 상기 바이오마커 유전자 서열의 전부 또는 일부를 포함하는 올리고뉴클레오티드 또는 이의 상보적인 올리고뉴클레오티드가 집적된 헥사클로로벤젠에 대한 노출 여부 확인용 마이크로어레이 칩을 제공한다.In addition, the present invention provides a microarray chip for confirming exposure to hexachlorobenzene in which an oligonucleotide including all or a part of the biomarker gene sequence or its complementary oligonucleotide is integrated.
또한, 본 발명은 상기 바이오마커를 이용한 헥사클로로벤젠에 대한 노출 여부 확인하기 위해 상기 바이오마커를 검출하는 방법을 제공한다.In addition, the present invention provides a method for detecting the biomarker to determine whether the biomarker is exposed to hexachlorobenzene.
아울러, 본 발명은 상기 바이오마커를 이용한 헥사클로로벤젠에 대한 노출 여부 확인용 키트를 제공한다.In addition, the present invention provides a kit for confirming exposure to hexachlorobenzene using the biomarker.
본 발명의 헥사클로로벤젠에 대한 노출 여부 확인용 바이오마커 및 이를 이 용한 확인방법은 DNA 마이크로어레이 칩을 통하여 선별된 반응 유전자들을 바이오마커로 이용하여 헥사클로로벤젠의 노출을 판정하는데 유용하며, 헥사클로로벤젠에 의해 야기되는 독성 작용 기작을 규명하는 도구로 이용할 수 있다.The biomarker for confirming the exposure to hexachlorobenzene of the present invention and the verification method using the same are useful for determining the exposure of hexachlorobenzene using reaction genes selected through DNA microarray chips as biomarkers. It can be used as a tool to identify the mechanism of toxic effects caused by benzene.
이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.
본 발명은 잔류성 유기 오염질인 헥사클로로벤젠(Hexachlorobenzene) 노출에 의해 발현 변화를 일으키는 것을 특징으로 하는 헥사클로로벤젠에 대한 노출 여부 확인용 바이오마커를 제공한다.The present invention provides a biomarker for confirming exposure to hexachlorobenzene, which is characterized by causing a change in expression by exposure to hexachlorobenzene, which is a persistent organic pollutant.
상기 바이오마커는 헥사클로로벤젠의 세포 생존 85%의 농도 처리군에서 발현이 변화된 유전자들로 p-값(p-value)이 0.001 이하에 해당하고, 1.5배 이상 발현이 증가 또는 0.667배 이하로 발현이 감소한 유전자로써, 유전자의 생물학적 방법(biological process)별 분류시 이에 해당하는 유전자의 기능은 스트레스반응(response to stress), 번역(translation), 지혈(hemostasis), 스테로이드 대사 과정(steroid metabolic process), 면역 작용자 과정(immune effector process), 섬유소 분해(fibrinolysis), 세포 분화(cell differentiation), 세포 자살(apoptosis), 지질 대사 과정(lipid metabolic process), 전사 규칙(positive regulation of transcription), 아미노산 대사 과정(amino acid metabolic process) 또는 스테롤 대사 과정(sterol metabolic process)과 관련 유전자로 구성 되어 있다.The biomarker is a gene whose expression is changed in the concentration treatment group of 85% cell survival of hexachlorobenzene, and the p-value corresponds to a p-value of 0.001 or less, and expression of 1.5 times or more is increased or is 0.667 times or less. With these reduced genes, the functions of these genes when classified by their biological methods are: stress to stress, translation, hemostasis, steroid metabolic process, Immune effector process, fibrinolysis, cell differentiation, apoptosis, lipid metabolic process, positive regulation of transcription, amino acid metabolism process It consists of amino acid metabolic process or sterol metabolic process and related genes.
본 발명은 하기와 같이 구성된 군에서 선택되는 것을 특징으로 하는 바이오마커를 제공한다:The present invention provides a biomarker characterized in that it is selected from the group consisting of:
유전자등록번호(GeneBank accession No.) AF011794(cell cycle progression 1), 유전자등록번호(GeneBank accession No.) AF118092(predicted protein of HQ2061; Homo sapiens PRO2061 mRNA, complete cds.), 유전자등록번호(GeneBank accession No.) AF119870(predicted protein of HQ2266; Homo sapiens PRO2266 mRNA, complete cds.), 유전자등록번호(GeneBank accession No.) AF274942(PNAS-17), 유전자등록번호(GeneBank accession No.) AI382322(Collagen, type XXVII, alpha 1), 유전자등록번호(GeneBank accession No.) AI570094[Transcribed locus, weakly similar to NP_033865.2 (Mus musculus)], 유전자등록번호(GeneBank accession No.) AI760454[Hairy and enhancer of split 2(Drosophila)], 유전자등록번호(GeneBank accession No.) AK054921(Cerebellar degeneration-related protein 1, 34kDa), 유전자등록번호(GeneBank accession No.) AK128263(Hypothetical protein MGC15523), 유전자등록번호(GeneBank accession No.) AK128714(Similar to embigin homolog), 유전자등록번호(GeneBank accession No.) BC006406(family with sequence similarity 104, member B), 유전자등록번호(GeneBank accession No.) BC009297(hypothetical protein FLJ22222), 유전자등록번호(GeneBank accession No.) BC012091[hairy and enhancer of split 2(Drosophila)], 유전자등록번호(GeneBank accession No.) BC032824[Homo sapiens chromosome 6 open reading frame 164, mRNA(cDNA clone IMAGE:4824064).], 유전자등록번호(GeneBank accession No.) BC045756(glycoprotein, alpha-galactosyltransferase 1), 유전자등록번호(GeneBank accession No.) CR615245[Similar to 40S ribosomal protein S3a(V-fos transformation effector protein)], 유전자등록번호(GeneBank accession No.) CR621148[Interleukin 6 signal transducer(gp130, oncostatin M receptor)], 유전자등록번호(GeneBank accession No.) CR627148(Homo sapiens, clone IMAGE:5213378, mRNA), 유전자등록번호(GeneBank accession No.) NM_000014(alpha-2-macroglobulin), 유전자등록번호(GeneBank accession No.) NM_000042[apolipoprotein H(beta-2-glycoprotein I)], 유전자등록번호(GeneBank accession No.) NM_000043[Fas(TNF receptor superfamily, member 6)], 유전자등록번호(GeneBank accession No.) NM_000130[coagulation factor V(proaccelerin, labile factor)], 유전자등록번호(GeneBank accession No.) NM_000242[mannose-binding lectin(protein C) 2, soluble(opsonic defect)], 유전자등록번호(GeneBank accession No.) NM_000301(plasminogen), 유전자등록번호(GeneBank accession No.) NM_000509(fibrinogen gamma chain), 유전자등록번호(GeneBank accession No.) NM_000582[secreted phosphoprotein 1(osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)], 유전자등록번호(GeneBank accession No.) NM_000633(B-cell CLL/lymphoma 2), 유전자등록번호(GeneBank accession No.) NM_000761(cytochrome P450, family 1, subfamily A, polypeptide 2), 유전자등록번호(GeneBank accession No.) NM_000846(glutathione S-transferase A2), 유전자등록번호(GeneBank accession No.) NM_000847(glutathione S-transferase A3), 유전자등록번호(GeneBank accession No.) NM_000859(3-hydroxy-3-methylglutaryl-Coenzyme A reductase), 유전자등록번호(GeneBank accession No.) NM_000997(ribosomal protein L37), 유전자등록번호(GeneBank accession No.) NM_001002236(serpin peptidase inhibitor, clade A(alpha-1 antiproteinase, antitrypsin), member 1), 유전자등록번호(GeneBank accession No.) NM_001014(ribosomal protein S10), 유전자등록번호(GeneBank accession No.) NM_001017(ribosomal protein S13), 유전자등록번호(GeneBank accession No.) NM_001072(UDP glucuronosyltransferase 1 family, polypeptide A6), 유전자등록번호(GeneBank accession No.) NM_001085[serpin peptidase inhibitor, clade A(alpha-1 antiproteinase, antitrypsin), member 3], 유전자등록번호(GeneBank accession No.) NM_001134(alpha-fetoprotein), 유전자등록번호(GeneBank accession No.) NM_001402(eukaryotic translation elongation factor 1 alpha 1), 유전자등록번호(GeneBank accession No.) NM_001443(fatty acid binding protein 1, liver), 유전자등록번호(GeneBank accession No.) NM_001568(eukaryotic translation initiation factor 3, subunit 6 48kDa), 유전자등록번호(GeneBank accession No.) NM_001793[cadherin 3, type 1, P-cadherin(placental)], 유전자등록번호(GeneBank accession No.) NM_001932[membrane protein, palmitoylated 3(MAGUK p55 subfamily member 3)], 유전자등록번호(GeneBank accession No.) NM_002130[3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1(soluble)], 유전자등록번호(GeneBank accession No.) NM_002210[integrin, alpha V(vitronectin receptor, alpha polypeptide, antigen CD51)], 유전자등록번호(GeneBank accession No.) NM_002567(phosphatidylethanolamine binding protein 1), 유전자등록번호(GeneBank accession No.) NM_002864(pregnancy-zone protein), 유전자등록번호(GeneBank accession No.) NM_003053[solute carrier family 18(vesicular monoamine), member 1], 유전자등록번호(GeneBank accession No.) NM_003126[spectrin, alpha, erythrocytic 1(elliptocytosis 2)], 유전자등록번호(GeneBank accession No.) NM_003262(translocation protein 1), 유전자등록번호(GeneBank accession No.) NM_003728[unc-5 homolog C(C. elegans)], 유전자등록번호(GeneBank accession No.) NM_003803(myomesin 1, 185kDa), 유전자등록번호(GeneBank accession No.) NM_003936[cyclin-dependent kinase 5, regulatory subunit 2(p39)], 유전자등록번호(GeneBank accession No.) NM_003947(kalirin, RhoGEF kinase), 유전자등록번호(GeneBank accession No.) NM_004048(beta-2-microglobulin), 유전자등록번호(GeneBank accession No.) NM_004066(centrin, EF-hand protein, 1), 유전자등록번호(GeneBank accession No.) NM_004164(retinol binding protein 2, cellular), 유전자등록번호(GeneBank accession No.) NM_004378(cellular retinoic acid binding protein 1), 유전자등록번호(GeneBank accession No.) NM_004508(isopentenyl-diphosphate delta isomerase 1), 유전자등 록번호(GeneBank accession No.) NM_004821(heart and neural crest derivatives expressed 1), 유전자등록번호(GeneBank accession No.) NM_005127(C-type lectin domain family 2, member B), 유전자등록번호(GeneBank accession No.) NM_005348[heat shock protein 90kDa alpha(cytosolic), class A member 1], 유전자등록번호(GeneBank accession No.) NM_005408[chemokine(C-C motif) ligand 13], 유전자등록번호(GeneBank accession No.) NM_005602[claudin 11(oligodendrocyte transmembrane protein)], 유전자등록번호(GeneBank accession No.) NM_006121[keratin 1(epidermolytic hyperkeratosis)], 유전자등록번호(GeneBank accession No.) NM_006136[capping protein(actin filament) muscle Z-line, alpha 2], 유전자등록번호(GeneBank accession No.) NM_006398(ubiquitin D), 유전자등록번호(GeneBank accession No.) NM_012135(family with sequence similarity 50, member B), 유전자등록번호(GeneBank accession No.) NM_012198(grancalcin, EF-hand calcium binding protein), 유전자등록번호(GeneBank accession No.) NM_014332(small muscle protein, X-linked), 유전자등록번호(GeneBank accession No.) NM_014619(glutamate receptor, ionotropic, kainate 4), 유전자등록번호(GeneBank accession No.) NM_016649[ESF1, nucleolar pre-rRNA processing protein, homolog(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_017493(OTU domain containing 4), 유전자등록번호(GeneBank accession No.) NM_017826(spermatogenesis and oogenesis specific basic helix-loop-helix 2), 유전자등록번호(GeneBank accession No.) NM_017855(odontogenic, ameloblast asssociated), 유전자등록번호(GeneBank accession No.) NM_019000(family with sequence similarity 134, member B), 유전자등록번호(GeneBank accession No.) NM_020805[kelch-like 14(Drosophila)], 유전자등록번호(GeneBank accession No.) NM_021009(ubiquitin C), 유전자등록번호(GeneBank accession No.) NM_021104(ribosomal protein L41), 유전자등록번호(GeneBank accession No.) NM_021631(apoptosis inhibitor), 유전자등록번호(GeneBank accession No.) NM_024665[transducin(beta)-like 1X-linked receptor 1], 유전자등록번호(GeneBank accession No.) NM_031308(epiplakin 1), 유전자등록번호(GeneBank accession No.) NM_053275(ribosomal protein, large, P0), 유전자등록번호(GeneBank accession No.) NM_138409(chromosome 6 open reading frame 117), 유전자등록번호(GeneBank accession No.) NM_139137(potassium voltage-gated channel, Shaw-related subfamily, member 2), 유전자등록번호(GeneBank accession No.) NM_145740(glutathione S-transferase A1), 유전자등록번호(GeneBank accession No.) NM_153699(glutathione S-transferase A5), 유전자등록번호(GeneBank accession No.) NM_153811(solute carrier family 38, member 6), 유전자등록번호(GeneBank accession No.) NM_173568(uromodulin-like 1), 유전자등록번호(GeneBank accession No.) NM_181054[hypoxia-inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor)], 유전자등록번호(GeneBank accession No.) NM_203304(ring finger and KH domain containing 1), 유전자등록번호(GeneBank accession No.) XM_498623{PREDICTED: Homo sapiens hypothetical LOC440309(LOC440309), mRNA [XM_498623]}, 유전자등록번호(GeneBank accession No.) XM_928021{Homo sapiens clone DNA147258 LMNE6487(UNQ6487) mRNA, complete cds. [AY358240]}, 유전자등록번호(GeneBank accession No.) XM_931362{PREDICTED: Homo sapiens similar to ribosomal protein S3a, transcript variant 3(LOC439992), mRNA [XM_931362]}, 유전자등록번호(GeneBank accession No.) XM_935867{Homo sapiens cDNA clone IMAGE:3621376, partial cds. [BC012751]}, 유전자등록번호(GeneBank accession No.) XM_940931(similar to fibrillarin), 유전자등록번호(GeneBank accession No.) AA449398(castor zinc finger 1), 유전자등록번호(GeneBank accession No.) AB040942(coiled-coil domain containing 88C), 유전자등록번호(GeneBank accession No.) AB065089(ribosomal protein S2), 유전자등록번호(GeneBank accession No.) AF005082(chromosome 1 open reading frame 46), 유전자등록번호(GeneBank accession No.) AF090927(Homo sapiens clone HQ0457 PRO0457 mRNA, complete cds.), 유전자등록번호(GeneBank accession No.) AF113013(HQ0806; Homo sapiens PRO0806 mRNA, complete cds.), 유전자등록번호(GeneBank accession No.) AF131778[discs, large(Drosophila) homolog-associated protein 3], 유전자등록번호(GeneBank accession No.) AF318368(hypothetical protein LOC650662), 유전자등록번호(GeneBank accession No.) AF323119[nuclear factor(erythroid-derived 2)-like 2], 유전자등록번호(GeneBank accession No.) AF357221(FKSG88), 유전자등록번호(GeneBank accession No.) AI500335[Bruno-like 5, RNA binding protein(Drosophila)], 유전 자등록번호(GeneBank accession No.) AI801539{Transcribed locus, moderately similar to XP_001109274.1 heat shock 27kDa protein 1 [Macaca mulatta]}, 유전자등록번호(GeneBank accession No.) AK001891(proline rich 11), 유전자등록번호(GeneBank accession No.) AK002097(hypothetical protein FLJ11235), 유전자등록번호(GeneBank accession No.) AK021860(F-box protein 17), 유전자등록번호(GeneBank accession No.) AK054771(v-akt murine thymoma viral oncogene homolog 2), 유전자등록번호(GeneBank accession No.) AK057432(IQ motif containing F3), 유전자등록번호(GeneBank accession No.) AK074121(platelet endothelial aggregation receptor 1), 유전자등록번호(GeneBank accession No.) AK074175[leucine-rich repeats and calponin homology(CH) domain containing 4], 유전자등록번호(GeneBank accession No.) AK074778(hypothetical protein FLJ90297), 유전자등록번호(GeneBank accession No.) AK092572(hypothetical protein LOC148413), 유전자등록번호(GeneBank accession No.) AK093729(Hypothetical gene supported by AK127273), 유전자등록번호(GeneBank accession No.) AK095727(CDNA FLJ38408 fis, clone FEBRA2009029), 유전자등록번호(GeneBank accession No.) AK095933(chromodomain helicase DNA binding protein 2), 유전자등록번호(GeneBank accession No.) AK096031(CDNA FLJ38712 fis, clone KIDNE2006733), 유전자등록번호(GeneBank accession No.) AK096643(hypothetical protein FLJ22184), 유전자등록번호(GeneBank accession No.) AK097259(Docking protein 3), 유전자등록번호(GeneBank accession No.) AK098372(Neuroblastoma breakpoint family, member 1), 유전자등록번호(GeneBank accession No.) AK123855(chromosome 1 open reading frame 170), 유전자등록번호(GeneBank accession No.) AK126622(WD repeat domain 90), 유전자등록번호(GeneBank accession No.) AK127768[MRNA; cDNA DKFZp547C1918(from clone DKFZp547C1918)], 유전자등록번호(GeneBank accession No.) AK128072(CDNA FLJ46193 fis, clone TESTI4006234), 유전자등록번호(GeneBank accession No.) AK129584[Full-length cDNA clone CS0DI007YH13 of Placenta Cot 25-normalized of Homo sapiens(human)], 유전자등록번호(GeneBank accession No.) AL050297(R3H domain and coiled-coil containing 1), 유전자등록번호(GeneBank accession No.) AL136837(hypothetical protein DKFZp434F142), 유전자등록번호(GeneBank accession No.) AL564305(Clusterin), 유전자등록번호(GeneBank accession No.) AL566332(Sorting nexin 26), 유전자등록번호(GeneBank accession No.) AX775899(unnamed protein product; Sequence 169 from Patent WO03048202.), 유전자등록번호(GeneBank accession No.) AY945262{Homo sapiens mucin(MUC8) mRNA, complete cds. [AY945262]}, 유전자등록번호(GeneBank accession No.) BC009872(branched chain ketoacid dehydrogenase kinase), 유전자등록번호(GeneBank accession No.) BC014228(prohibitin pseudogene), 유전자등록번호(GeneBank accession No.) BC014606(CDNA clone IMAGE:4826808), 유전자등록번호(GeneBank accession No.) BC016140[mevalonate kinase(mevalonic aciduria)], 유전자등록번호(GeneBank accession No.) BC018035(CDNA clone IMAGE:4819956), 유 전자등록번호(GeneBank accession No.) BC021118(paroxysmal nonkinesiogenic dyskinesia), 유전자등록번호(GeneBank accession No.) BC022394(zinc finger protein 493), 유전자등록번호(GeneBank accession No.) BC022562(CDNA clone IMAGE:4794059), 유전자등록번호(GeneBank accession No.) BC025310(leucine rich repeat and fibronectin type III domain containing 1), 유전자등록번호(GeneBank accession No.) BC027988(hypothetical protein LOC253842), 유전자등록번호(GeneBank accession No.) BC033110[hydroxysteroid(17-beta) dehydrogenase 1], 유전자등록번호(GeneBank accession No.) BC042034(chromosome 7 open reading frame 46), 유전자등록번호(GeneBank accession No.) BC062790(hypothetical gene supported by BC038466; BC062790), 유전자등록번호(GeneBank accession No.) BC063316(F-box and leucine-rich repeat protein 17), 유전자등록번호(GeneBank accession No.) BC080624(Similar to Rho GTPase activating protein 15), 유전자등록번호(GeneBank accession No.) BC092421(Hypothetical gene supported by AK127273), 유전자등록번호(GeneBank accession No.) BC106086(chromosome 20 open reading frame 194), 유전자등록번호(GeneBank accession No.) BC110642(p20), 유전자등록번호(GeneBank accession No.) BG259069(Transcribed locus), 유전자등록번호(GeneBank accession No.) BG620990(Similar to ATP binding domain 3), 유전자등록번호(GeneBank accession No.) BM054818(Mitochondrial ribosomal protein S26), 유전자등록번호(GeneBank accession No.) BU633383(UI-H-FL1-bgu-h-03-0-UI.s1 NCI_CGAP_FL1 Homo sapiens cDNA clone UI-H-FL1-bgu-h-03-0-UI 3', mRNA sequence.), 유전자등록번호(GeneBank accession No.) BX104999(Transcribed locus), 유전자등록번호(GeneBank accession No.) BX111592(Transcribed locus), 유전자등록번호(GeneBank accession No.) BX648950[MRNA; cDNA DKFZp686E0389(from clone DKFZp686E0389)], 유전자등록번호(GeneBank accession No.) CR600494(Hypothetical protein BC006130), 유전자등록번호(GeneBank accession No.) CR601391(Chromosome 1 open reading frame 144), 유전자등록번호(GeneBank accession No.) CR609146[ATP-binding cassette, sub-family C(CFTR/MRP), member 6], 유전자등록번호(GeneBank accession No.) CR936831(C8orfK32 protein), 유전자등록번호(GeneBank accession No.) CV402334[Similar to Adenosylhomocysteinase(S-adenosyl-L-homocysteine hydrolase)(AdoHcyase)], 유전자등록번호(GeneBank accession No.) DA072538(Transcribed locus), 유전자등록번호(GeneBank accession No.) J05272[IMP(inosine monophosphate) dehydrogenase 1], 유전자등록번호(GeneBank accession No.) M94173(calcium channel, voltage-dependent, N type, alpha 1B subunit), 유전자등록번호(GeneBank accession No.) NM_000189(hexokinase 2), 유전자등록번호(GeneBank accession No.) NM_000195(Hermansky-Pudlak syndrome 1), 유전자등록번호(GeneBank accession No.) NM_000221[ketohexokinase(fructokinase)], 유전자등록번호(GeneBank accession No.) NM_000229(lecithin-cholesterol acyltransferase), 유전자등록번호(GeneBank accession No.) NM_000263[N-acetylglucosaminidase, alpha-(Sanfilippo disease IIIB)], 유전자등록번호(GeneBank accession No.) NM_000302(procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1), 유전자등록번호(GeneBank accession No.) NM_000303(phosphomannomutase 2), 유전자등록번호(GeneBank accession No.) NM_000403(UDP-galactose-4-epimerase), 유전자등록번호(GeneBank accession No.) NM_000457(hepatocyte nuclear factor 4, alpha), 유전자등록번호(GeneBank accession No.) NM_000481(aminomethyltransferase), 유전자등록번호(GeneBank accession No.) NM_000495[collagen, type IV, alpha 5(Alport syndrome)], 유전자등록번호(GeneBank accession No.) NM_000594[tumor necrosis factor(TNF superfamily, member 2)], 유전자등록번호(GeneBank accession No.) NM_000638(vitronectin), 유전자등록번호(GeneBank accession No.) NM_000709(branched chain keto acid dehydrogenase E1, alpha polypeptide), 유전자등록번호(GeneBank accession No.) NM_000713{biliverdin reductase B [flavin reductase(NADPH)]}, 유전자등록번호(GeneBank accession No.) NM_000789[angiotensin I converting enzyme(peptidyl-dipeptidase A) 1], 유전자등록번호(GeneBank accession No.) NM_000841(glutamate receptor, metabotropic 4), 유전자등록번호(GeneBank accession No.) NM_000876(insulin-like growth factor 2 receptor), 유전자등록번호(GeneBank accession No.) NM_000918[procollagen-proline, 2-oxoglutarate 4-dioxygenase(proline 4-hydroxylase), beta polypeptide], 유전자등록번호(GeneBank accession No.) NM_000923[phosphodiesterase 4C, cAMP-specific(phosphodiesterase E1 dunce homolog, Drosophila)], 유전자등록번호(GeneBank accession No.) NM_000991(ribosomal protein L28), 유전자등록번호(GeneBank accession No.) NM_001001290[solute carrier family 2(facilitated glucose transporter), member 9], 유전자등록번호(GeneBank accession No.) NM_001001975(ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit), 유전자등록번호(GeneBank accession No.) NM_001004313(similar to RIKEN cDNA A730055C05 gene), 유전자등록번호(GeneBank accession No.) NM_001004349(FLJ45422 protein), 유전자등록번호(GeneBank accession No.) NM_001004356(fibroblast growth factor receptor-like 1), 유전자등록번호(GeneBank accession No.) NM_001005209(similar to RIKEN cDNA A230078I05 gene), 유전자등록번호(GeneBank accession No.) NM_001007125(bA299N6.3), 유전자등록번호(GeneBank accession No.) NM_001008397(similar to RIKEN cDNA 2310016C16), 유전자등록번호(GeneBank accession No.) NM_001013(ribosomal protein S9), 유전자등록번호(GeneBank accession No.) NM_001013839(exocyst complex component 7), 유전자등록번호(GeneBank accession No.) NM_001014763(electron-transfer-flavoprotein, beta polypeptide), 유전자등록번호(GeneBank accession No.) NM_001014765(F-box protein 44), 유전자등록번호(GeneBank accession No.) NM_001017418(small proline-rich protein 2B), 유전자등록번호(GeneBank accession No.) NM_001025200(chymotrypsinogen B2), 유전자등록번호(GeneBank accession No.) NM_001033515(similar to hypothetical protein MGC27019), 유전자등록번호(GeneBank accession No.) NM_001035235(steroid receptor RNA activator 1), 유 전자등록번호(GeneBank accession No.) NM_001037125[unkempt homolog(Drosophila)-like], 유전자등록번호(GeneBank accession No.) NM_001039464(chromosome 1 open reading frame 175), 유전자등록번호(GeneBank accession No.) NM_001039476(chromosome 16 open reading frame 35), 유전자등록번호(GeneBank accession No.) NM_001039842(hypothetical protein LOC339229), 유전자등록번호(GeneBank accession No.) NM_001040078(similar to galectin 9 short isoform), 유전자등록번호(GeneBank accession No.) NM_001064[transketolase(Wernicke-Korsakoff syndrome)], 유전자등록번호(GeneBank accession No.) NM_001070(tubulin, gamma 1), 유전자등록번호(GeneBank accession No.) NM_001087(angio-associated, migratory cell protein), 유전자등록번호(GeneBank accession No.) NM_001098(aconitase 2, mitochondrial), 유전자등록번호(GeneBank accession No.) NM_001102(actinin, alpha 1), 유전자등록번호(GeneBank accession No.) NM_001106(activin A receptor, type IIB), 유전자등록번호(GeneBank accession No.) NM_001152[solute carrier family 25(mitochondrial carrier; adenine nucleotide translocator), member 5], 유전자등록번호(GeneBank accession No.) NM_001155(annexin A6), 유전자등록번호(GeneBank accession No.) NM_001188(BCL2-antagonist/killer 1), 유전자등록번호(GeneBank accession No.) NM_001191(BCL2-like 1), 유전자등록번호(GeneBank accession No.) NM_001250(CD40 molecule, TNF receptor superfamily member 5), 유전자등록번호(GeneBank accession No.) NM_001273(chromodomain helicase DNA binding protein 4), 유전자등록번호(GeneBank accession No.) NM_001286(chloride channel 6), 유전자등록번호(GeneBank accession No.) NM_001348(death-associated protein kinase 3), 유전자등록번호(GeneBank accession No.) NM_001386(dihydropyrimidinase-like 2), 유전자등록번호(GeneBank accession No.) NM_001388(developmentally regulated GTP binding protein 2), 유전자등록번호(GeneBank accession No.) NM_001398(enoyl Coenzyme A hydratase 1, peroxisomal), 유전자등록번호(GeneBank accession No.) NM_001440[exostoses(multiple)-like 3], 유전자등록번호(GeneBank accession No.) NM_001455(forkhead box O3), 유전자등록번호(GeneBank accession No.) NM_001521(general transcription factor IIIC, polypeptide 2, beta 110kDa), 유전자등록번호(GeneBank accession No.) NM_001524[hypocretin(orexin) neuropeptide precursor], 유전자등록번호(GeneBank accession No.) NM_001543[N-deacetylase/N-sulfotransferase(heparan glucosaminyl) 1], 유전자등록번호(GeneBank accession No.) NM_001614(actin, gamma 1), 유전자등록번호(GeneBank accession No.) NM_001620(AHNAK nucleoprotein), 유전자등록번호(GeneBank accession No.) NM_001636[solute carrier family 25(mitochondrial carrier; adenine nucleotide translocator), member 6], 유전자등록번호(GeneBank accession No.) NM_001654(v-raf murine sarcoma 3611 viral oncogene homolog), 유전자등록번호(GeneBank accession No.) NM_001659(ADP-ribosylation factor 3), 유전자등록번호(GeneBank accession No.) NM_001661(ADP-ribosylation factor-like 4D), 유전자등록번호(GeneBank accession No.) NM_001662(ADP-ribosylation factor 5), 유전자등록번호(GeneBank accession No.) NM_001684(ATPase, Ca++ transporting, plasma membrane 4), 유전자등록번호(GeneBank accession No.) NM_001728[basigin(Ok blood group)], 유전자등록번호(GeneBank accession No.) NM_001789[cell division cycle 25 homolog A(S. pombe)], 유전자등록번호(GeneBank accession No.) NM_001823(creatine kinase, brain), 유전자등록번호(GeneBank accession No.) NM_001887(crystallin, beta B1), 유전자등록번호(GeneBank accession No.) NM_001964(early growth response 1), 유전자등록번호(GeneBank accession No.) NM_001978[erythrocyte membrane protein band 4.9(dematin)], 유전자등록번호(GeneBank accession No.) NM_001986[ets variant gene 4(E1A enhancer binding protein, E1AF)], 유전자등록번호(GeneBank accession No.) NM_001997[Finkel-Biskis-Reilly murine sarcoma virus(FBR-MuSV) ubiquitously expressed(fox derived); ribosomal protein S30], 유전자등록번호(GeneBank accession No.) NM_002005(feline sarcoma oncogene), 유전자등록번호(GeneBank accession No.) NM_002046(glyceraldehyde-3-phosphate dehydrogenase), 유전자등록번호(GeneBank accession No.) NM_002060(gap junction protein, alpha 4, 37kDa), 유전자등록번호(GeneBank accession No.) NM_002086(growth factor receptor-bound protein 2), 유전자등록번호(GeneBank accession No.) NM_002119(major histocompatibility complex, class II, DO alpha), 유전자등록번호(GeneBank accession No.) NM_002307[lectin, galactoside- binding, soluble, 7(galectin 7)], 유전자등록번호(GeneBank accession No.) NM_002317(lysyl oxidase), 유전자등록번호(GeneBank accession No.) NM_002332[low density lipoprotein-related protein 1(alpha-2-macroglobulin receptor)], 유전자등록번호(GeneBank accession No.) NM_002333(low density lipoprotein receptor-related protein 3), 유전자등록번호(GeneBank accession No.) NM_002359[v-maf musculoaponeurotic fibrosarcoma oncogene homolog G(avian)], 유전자등록번호(GeneBank accession No.) NM_002415[macrophage migration inhibitory factor(glycosylation-inhibiting factor)], 유전자등록번호(GeneBank accession No.) NM_002457(mucin 2, oligomeric mucus/gel-forming), 유전자등록번호(GeneBank accession No.) NM_002466[v-myb myeloblastosis viral oncogene homolog(avian)-like 2], 유전자등록번호(GeneBank accession No.) NM_002468[myeloid differentiation primary response gene(88)], 유전자등록번호(GeneBank accession No.) NM_002542(8-oxoguanine DNA glycosylase), 유전자등록번호(GeneBank accession No.) NM_002573(platelet-activating factor acetylhydrolase, isoform Ib, gamma subunit 29kDa), 유전자등록번호(GeneBank accession No.) NM_002591[phosphoenolpyruvate carboxykinase 1(soluble)], 유전자등록번호(GeneBank accession No.) NM_002650(phosphatidylinositol 4-kinase, catalytic, alpha polypeptide), 유전자등록번호(GeneBank accession No.) NM_002660(phospholipase C, gamma 1), 유전자등록번호(GeneBank accession No.) NM_002720[protein phosphatase 4(formerly X), catalytic subunit], 유전자등록번 호(GeneBank accession No.) NM_002804[proteasome(prosome, macropain) 26S subunit, ATPase, 3], 유전자등록번호(GeneBank accession No.) NM_002821(PTK7 protein tyrosine kinase 7), 유전자등록번호(GeneBank accession No.) NM_002840(protein tyrosine phosphatase, receptor type, F), 유전자등록번호(GeneBank accession No.) NM_002862(phosphorylase, glycogen; brain), 유전자등록번호(GeneBank accession No.) NM_002883(Ran GTPase activating protein 1), 유전자등록번호(GeneBank accession No.) NM_002996[chemokine(C-X3-C motif) ligand 1], 유전자등록번호(GeneBank accession No.) NM_003003[SEC14-like 1(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_003029[SHC(Src homology 2 domain containing) transforming protein 1], 유전자등록번호(GeneBank accession No.) NM_003056{Homo sapiens solute carrier family 19(folate transporter), member 1(SLC19A1), transcript variant 1, mRNA [NM_003056]}, 유전자등록번호(GeneBank accession No.) NM_003061[slit homolog 1(Drosophila)], 유전자등록번호(GeneBank accession No.) NM_003062[slit homolog 3(Drosophila)], 유전자등록번호(GeneBank accession No.) NM_003123[sialophorin(leukosialin, CD43)], 유전자등록번호(GeneBank accession No.) NM_003170[suppressor of Ty 6 homolog(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_003204[nuclear factor(erythroid-derived 2)-like 1], 유전자등록번호(GeneBank accession No.) NM_003213(TEA domain family member 4), 유전자등록번호(GeneBank accession No.) NM_003216(thyrotrophic embryonic factor), 유전자등록번호(GeneBank accession No.) NM_003224(ADP-ribosylation factor related protein 1), 유전자등록번호(GeneBank accession No.) NM_003226[trefoil factor 3(intestinal)], 유전자등록번호(GeneBank accession No.) NM_003258(thymidine kinase 1, soluble), 유전자등록번호(GeneBank accession No.) NM_003259(intercellular adhesion molecule 5, telencephalin), 유전자등록번호(GeneBank accession No.) NM_003277[claudin 5(transmembrane protein deleted in velocardiofacial syndrome)], 유전자등록번호(GeneBank accession No.) NM_003388(CAP-GLY domain containing linker protein 2), 유전자등록번호(GeneBank accession No.) NM_003486[solute carrier family 7(cationic amino acid transporter, y+ system), member 5], 유전자등록번호(GeneBank accession No.) NM_003501(acyl-Coenzyme A oxidase 3, pristanoyl), 유전자등록번호(GeneBank accession No.) NM_003513(histone cluster 1, H2ab), 유전자등록번호(GeneBank accession No.) NM_003633[ectodermal-neural cortex(with BTB-like domain)], 유전자등록번호(GeneBank accession No.) NM_003634[nipsnap homolog 1(C. elegans)], 유전자등록번호(GeneBank accession No.) NM_003683[ribosomal RNA processing 1 homolog(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_003695(lymphocyte antigen 6 complex, locus D), 유전자등록번호(GeneBank accession No.) NM_003748(aldehyde dehydrogenase 4 family, member A1), 유전자등록번호(GeneBank accession No.) NM_003751(eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa), 유전자등록번호(GeneBank accession No.) NM_003791(membrane-bound transcription factor peptidase, site 1), 유전자등록번호(GeneBank accession No.) NM_003801[glycosylphosphatidylinositol anchor attachment protein 1 homolog(yeast)], 유전자등록번호(GeneBank accession No.) NM_003891(protein Z, vitamin K-dependent plasma glycoprotein), 유전자등록번호(GeneBank accession No.) NM_003926(methyl-CpG binding domain protein 3), 유전자등록번호(GeneBank accession No.) NM_003984[solute carrier family 13(sodium-dependent dicarboxylate transporter), member 2], 유전자등록번호(GeneBank accession No.) NM_004082[dynactin 1(p150, glued homolog, Drosophila)], 유전자등록번호(GeneBank accession No.) NM_004090[dual specificity phosphatase 3(vaccinia virus phosphatase VH1-related)], 유전자등록번호(GeneBank accession No.) NM_004168[succinate dehydrogenase complex, subunit A, flavoprotein(Fp)], 유전자등록번호(GeneBank accession No.) NM_004169[serine hydroxymethyltransferase 1(soluble)], 유전자등록번호(GeneBank accession No.) NM_004214[fibroblast growth factor(acidic) intracellular binding protein], 유전자등록번호(GeneBank accession No.) NM_004286(GTP binding protein 1), 유전자등록번호(GeneBank accession No.) NM_004309[Rho GDP dissociation inhibitor(GDI) alpha], 유전자등록번호(GeneBank accession No.) NM_004341(carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase), 유전자등록번호(GeneBank accession No.) NM_004420(dual specificity phosphatase 8), 유전자등록번 호(GeneBank accession No.) NM_004436(endosulfine alpha), 유전자등록번호(GeneBank accession No.) NM_004475(flotillin 2), 유전자등록번호(GeneBank accession No.) NM_004491(glucocorticoid receptor DNA binding factor 1), 유전자등록번호(GeneBank accession No.) NM_004494[hepatoma-derived growth factor(high-mobility group protein 1-like)], 유전자등록번호(GeneBank accession No.) NM_004563[phosphoenolpyruvate carboxykinase 2(mitochondrial)], 유전자등록번호(GeneBank accession No.) NM_004566(6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3), 유전자등록번호(GeneBank accession No.) NM_004567(6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4), 유전자등록번호(GeneBank accession No.) NM_004604(syntaxin 4), 유전자등록번호(GeneBank accession No.) NM_004609[transcription factor 15(basic helix-loop-helix)], 유전자등록번호(GeneBank accession No.) NM_004639(HLA-B associated transcript 3), 유전자등록번호(GeneBank accession No.) NM_004651(ubiquitin specific peptidase 11), 유전자등록번호(GeneBank accession No.) NM_004656[BRCA1 associated protein-1(ubiquitin carboxy-terminal hydrolase)], 유전자등록번호(GeneBank accession No.) NM_004785[solute carrier family 9(sodium/hydrogen exchanger), member 3 regulator 2], 유전자등록번호(GeneBank accession No.) NM_004822(netrin 1), 유전자등록번호(GeneBank accession No.) NM_005018(programmed cell death 1), 유전자등록번호(GeneBank accession No.) NM_005077[transducin-like enhancer of split 1(E(sp1) homolog, Drosophila)], 유전자등록번호(GeneBank accession No.) NM_005082(tripartite motif-containing 25), 유전자등록번호(GeneBank accession No.) NM_005104(bromodomain containing 2), 유전자등록번호(GeneBank accession No.) NM_005119(thyroid hormone receptor associated protein 3), 유전자등록번호(GeneBank accession No.) NM_005163(v-akt murine thymoma viral oncogene homolog 1), 유전자등록번호(GeneBank accession No.) NM_005184[calmodulin 3(phosphorylase kinase, delta)], 유전자등록번호(GeneBank accession No.) NM_005216(dolichyl-diphosphooligosaccharide-protein glycosyltransferase), 유전자등록번호(GeneBank accession No.) NM_005258(GTP cyclohydrolase I feedback regulator), 유전자등록번호(GeneBank accession No.) NM_005338(huntingtin interacting protein 1), 유전자등록번호(GeneBank accession No.) NM_005412[serine hydroxymethyltransferase 2(mitochondrial)], 유전자등록번호(GeneBank accession No.) NM_005417[v-src sarcoma(Schmidt-Ruppin A-2) viral oncogene homolog(avian)], 유전자등록번호(GeneBank accession No.) NM_005468(N-acetylated alpha-linked acidic dipeptidase-like 1), 유전자등록번호(GeneBank accession No.) NM_005469(acyl-CoA thioesterase 8), 유전자등록번호(GeneBank accession No.) NM_005477(hyperpolarization activated cyclic nucleotide-gated potassium channel 4), 유전자등록번호(GeneBank accession No.) NM_005514(major histocompatibility complex, class I, B), 유전자등록번호(GeneBank accession No.) NM_005532(interferon, alpha-inducible protein 27), 유전자등록번 호(GeneBank accession No.) NM_005547(involucrin), 유전자등록번호(GeneBank accession No.) NM_005572(lamin A/C), 유전자등록번호(GeneBank accession No.) NM_005581[basal cell adhesion molecule(Lutheran blood group)], 유전자등록번호(GeneBank accession No.) NM_005619(reticulon 2), 유전자등록번호(GeneBank accession No.) NM_005735[ARP1 actin-related protein 1 homolog B, centractin beta(yeast)], 유전자등록번호(GeneBank accession No.) NM_005762(tripartite motif-containing 28), 유전자등록번호(GeneBank accession No.) NM_005881(branched chain ketoacid dehydrogenase kinase), 유전자등록번호(GeneBank accession No.) NM_005934[myeloid/lymphoid or mixed-lineage leukemia(trithorax homolog, Drosophila); translocated to, 1], 유전자등록번호(GeneBank accession No.) NM_005954(metallothionein 3), 유전자등록번호(GeneBank accession No.) NM_005964(myosin, heavy chain 10, non-muscle), 유전자등록번호(GeneBank accession No.) NM_005967[NGFI-A binding protein 2(EGR1 binding protein 2)], 유전자등록번호(GeneBank accession No.) NM_005987(small proline-rich protein 1A), 유전자등록번호(GeneBank accession No.) NM_006000(tubulin, alpha 4a), 유전자등록번호(GeneBank accession No.) NM_006005[Wolfram syndrome 1(wolframin)], 유전자등록번호(GeneBank accession No.) NM_006012[ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog(E. coli)], 유전자등록번호(GeneBank accession No.) NM_006051[amyloid beta(A4) precursor protein-binding, family B, member 3], 유 전자등록번호(GeneBank accession No.) NM_006058(TNFAIP3 interacting protein 1), 유전자등록번호(GeneBank accession No.) NM_006062(SMYD family member 5), 유전자등록번호(GeneBank accession No.) NM_006099(protein inhibitor of activated STAT, 3), 유전자등록번호(GeneBank accession No.) NM_006230[polymerase(DNA directed), delta 2, regulatory subunit 50kDa], 유전자등록번호(GeneBank accession No.) NM_006234[polymerase(RNA) II(DNA directed) polypeptide J, 13.3kDa], 유전자등록번호(GeneBank accession No.) NM_006266(ral guanine nucleotide dissociation stimulator), 유전자등록번호(GeneBank accession No.) NM_006295(valyl-tRNA synthetase), 유전자등록번호(GeneBank accession No.) NM_006302(glucosidase I), 유전자등록번호(GeneBank accession No.) NM_006322(tubulin, gamma complex associated protein 3), 유전자등록번호(GeneBank accession No.) NM_006328(RNA binding motif protein 14), 유전자등록번호(GeneBank accession No.) NM_006337(microspherule protein 1), 유전자등록번호(GeneBank accession No.) NM_006341[MAD2 mitotic arrest deficient-like 2(yeast)], 유전자등록번호(GeneBank accession No.) NM_006427(SIVA1, apoptosis-inducing factor), 유전자등록번호(GeneBank accession No.) NM_006429[chaperonin containing TCP1, subunit 7(eta)], 유전자등록번호(GeneBank accession No.) NM_006454(MAX dimerization protein 4), 유전자등록번호(GeneBank accession No.) NM_006464(trans-golgi network protein 2), 유전자등록번호(GeneBank accession No.) NM_006516[solute carrier family 2(facilitated glucose transporter), member 1], 유전자등록번호(GeneBank accession No.) NM_006546(insulin-like growth factor 2 mRNA binding protein 1), 유전자등록번호(GeneBank accession No.) NM_006556(phosphomevalonate kinase), 유전자등록번호(GeneBank accession No.) NM_006565[CCCTC-binding factor(zinc finger protein)], 유전자등록번호(GeneBank accession No.) NM_006623(phosphoglycerate dehydrogenase), 유전자등록번호(GeneBank accession No.) NM_006653(fibroblast growth factor receptor substrate 3), 유전자등록번호(GeneBank accession No.) NM_006663[protein phosphatase 1, regulatory(inhibitor) subunit 13 like], 유전자등록번호(GeneBank accession No.) NM_006753(surfeit 6), 유전자등록번호(GeneBank accession No.) NM_006755(transaldolase 1), 유전자등록번호(GeneBank accession No.) NM_006762(lysosomal associated multispanning membrane protein 5), 유전자등록번호(GeneBank accession No.) NM_006801[KDEL(Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1], 유전자등록번호(GeneBank accession No.) NM_006814[proteasome(prosome, macropain) inhibitor subunit 1(PI31)], 유전자등록번호(GeneBank accession No.) NM_006842(splicing factor 3b, subunit 2, 145kDa), 유전자등록번호(GeneBank accession No.) NM_006858(transmembrane emp24 protein transport domain containing 1), 유전자등록번호(GeneBank accession No.) NM_006929[superkiller viralicidic activity 2-like(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_006931[solute carrier family 2(facilitated glucose transporter), member 3], 유전자등록번호(GeneBank accession No.) NM_007024(transmembrane protein 115), 유전자등록번호(GeneBank accession No.) NM_007059[kaptin(actin binding protein)], 유전자등록번호(GeneBank accession No.) NM_007286(synaptopodin), 유전자등록번호(GeneBank accession No.) NM_007317(kinesin family member 22), 유전자등록번호(GeneBank accession No.) NM_007346(opioid growth factor receptor), 유전자등록번호(GeneBank accession No.) NM_007353[guanine nucleotide binding protein(G protein) alpha 12], 유전자등록번호(GeneBank accession No.) NM_012162(F-box and leucine-rich repeat protein 6), 유전자등록번호(GeneBank accession No.) NM_012218(interleukin enhancer binding factor 3, 90kDa), 유전자등록번호(GeneBank accession No.) NM_012227[GTP binding protein 6(putative)], 유전자등록번호(GeneBank accession No.) NM_012334(myosin X), 유전자등록번호(GeneBank accession No.) NM_012398(phosphatidylinositol-4-phosphate 5-kinase, type I, gamma), 유전자등록번호(GeneBank accession No.) NM_012404[acidic(leucine-rich) nuclear phosphoprotein 32 family, member D], 유전자등록번호(GeneBank accession No.) NM_012426(splicing factor 3b, subunit 3, 130kDa), 유전자등록번호(GeneBank accession No.) NM_012458[translocase of inner mitochondrial membrane 13 homolog(yeast)], 유전자등록번호(GeneBank accession No.) NM_012477(WW domain binding protein 1), 유전자등록번호(GeneBank accession No.) NM_012478(WW domain binding protein 2), 유전자등록번호(GeneBank accession No.) NM_013282(ubiquitin-like, containing PHD and RING finger domains, 1), 유전자등록번호(GeneBank accession No.) NM_013328(pyrroline-5-carboxylate reductase family, member 2), 유전자등록번호(GeneBank accession No.) NM_013333(epsin 1), 유전자등록번호(GeneBank accession No.) NM_013356[solute carrier family 16, member 8(monocarboxylic acid transporter 3)], 유전자등록번호(GeneBank accession No.) NM_013379(dipeptidyl-peptidase 7), 유전자등록번호(GeneBank accession No.) NM_013389[NPC1(Niemann-Pick disease, type C1, gene)-like 1], 유전자등록번호(GeneBank accession No.) NM_013392(nuclear receptor binding protein 1), 유전자등록번호(GeneBank accession No.) NM_013407(deoxyhypusine synthase), 유전자등록번호(GeneBank accession No.) NM_013430(gamma-glutamyltransferase 1), 유전자등록번호(GeneBank accession No.) NM_013442[stomatin(EPB72)-like 2], 유전자등록번호(GeneBank accession No.) NM_013976(glutaryl-Coenzyme A dehydrogenase), 유전자등록번호(GeneBank accession No.) NM_014007(zinc finger and BTB domain containing 43), 유전자등록번호(GeneBank accession No.) NM_014064(chromosome 9 open reading frame 32), 유전자등록번호(GeneBank accession No.) NM_014225[protein phosphatase 2(formerly 2A), regulatory subunit A , alpha isoform], 유전자등록번호(GeneBank accession No.) NM_014281(fuse-binding protein-interacting repressor), 유전자등록번호(GeneBank accession No.) NM_014316(calcium regulated heat stable protein 1, 24kDa), 유전자등록번호(GeneBank accession No.) NM_014328(RUN and SH3 domain containing 1), 유전자 등록번호(GeneBank accession No.) NM_014341[mitochondrial carrier homolog 1(C. elegans)], 유전자등록번호(GeneBank accession No.) NM_014392[DNA segment on chromosome 4(unique) 234 expressed sequence], 유전자등록번호(GeneBank accession No.) NM_014437[solute carrier family 39(zinc transporter), member 1], 유전자등록번호(GeneBank accession No.) NM_014551(non-SMC condensin II complex, subunit H2), 유전자등록번호(GeneBank accession No.) NM_014587[SRY(sex determining region Y)-box 8], 유전자등록번호(GeneBank accession No.) NM_014675(ciliary rootlet coiled-coil, rootletin), 유전자등록번호(GeneBank accession No.) NM_014678(SAPS domain family, member 2), 유전자등록번호(GeneBank accession No.) NM_014706(squamous cell carcinoma antigen recognized by T cells 3), 유전자등록번호(GeneBank accession No.) NM_014719(KIAA0738 gene product), 유전자등록번호(GeneBank accession No.) NM_014727(myeloid/lymphoid or mixed-lineage leukemia 4), 유전자등록번호(GeneBank accession No.) NM_014738(KIAA0195), 유전자등록번호(GeneBank accession No.) NM_014748(sorting nexin 17), 유전자등록번호(GeneBank accession No.) NM_014762(24-dehydrocholesterol reductase), 유전자등록번호(GeneBank accession No.) NM_014780(cullin 7), 유전자등록번호(GeneBank accession No.) NM_014952(bromo adjacent homology domain containing 1), 유전자등록번호(GeneBank accession No.) NM_015024(exportin 7), 유전자등록번호(GeneBank accession No.) NM_015075(IQ motif and Sec7 domain 2), 유전자등록번호(GeneBank accession No.) NM_015111(Nedd4 binding protein 3), 유전자등록번호(GeneBank accession No.) NM_015171(exportin 6), 유전자등록번호(GeneBank accession No.) NM_015198[cordon-bleu homolog(mouse)], 유전자등록번호(GeneBank accession No.) NM_015289[vacuolar protein sorting 39 homolog(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_015319[tensin like C1 domain containing phosphatase(tensin 2)], 유전자등록번호(GeneBank accession No.) NM_015353(potassium channel tetramerisation domain containing 2), 유전자등록번호(GeneBank accession No.) NM_015354(nucleoporin 188kDa), 유전자등록번호(GeneBank accession No.) NM_015466(protein tyrosine phosphatase, non-receptor type 23), 유전자등록번호(GeneBank accession No.) NM_015517(MBD2-interacting zinc finger), 유전자등록번호(GeneBank accession No.) NM_015528(ring finger protein 167), 유전자등록번호(GeneBank accession No.) NM_015533[dihydroxyacetone kinase 2 homolog(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_015603(coiled-coil domain containing 9), 유전자등록번호(GeneBank accession No.) NM_015853(SAPK substrate protein 1), 유전자등록번호(GeneBank accession No.) NM_015914(thioredoxin domain containing 11), 유전자등록번호(GeneBank accession No.) NM_015926(testis expressed 264), 유전자등록번호(GeneBank accession No.) NM_015950(mitochondrial ribosomal protein L2), 유전자등록번호(GeneBank accession No.) NM_015953(nitric oxide synthase interacting protein), 유전자등록번호(GeneBank accession No.) NM_015965[NADH dehydrogenase(ubiquinone) 1 alpha subcomplex, 13], 유전자등록번호(GeneBank accession No.) NM_016022[anterior pharynx defective 1 homolog A(C. elegans)], 유전자등록번호(GeneBank accession No.) NM_016332(selenoprotein X, 1), 유전자등록번호(GeneBank accession No.) NM_016437(tubulin, gamma 2), 유전자등록번호(GeneBank accession No.) NM_016438(HIG1 domain family, member 1B), 유전자등록번호(GeneBank accession No.) NM_016492(RAN guanine nucleotide release factor), 유전자등록번호(GeneBank accession No.) NM_016564(cell cycle exit and neuronal differentiation 1), 유전자등록번호(GeneBank accession No.) NM_016733(LIM domain kinase 2), 유전자등록번호(GeneBank accession No.) NM_017422(calmodulin-like 5), 유전자등록번호(GeneBank accession No.) NM_017491(WD repeat domain 1), 유전자등록번호(GeneBank accession No.) NM_017510(transmembrane emp24 protein transport domain containing 9), 유전자등록번호(GeneBank accession No.) NM_017626(DnaJ(Hsp40) homolog, subfamily B, member 12), 유전자등록번호(GeneBank accession No.) NM_017668[nudE nuclear distribution gene E homolog 1(A. nidulans)], 유전자등록번호(GeneBank accession No.) NM_017702(hypothetical protein FLJ20186), 유전자등록번호(GeneBank accession No.) NM_017727(hypothetical protein FLJ20254), 유전자등록번호(GeneBank accession No.) NM_017750[retinol saturase(all-trans-retinol 13,14-reductase)], 유전자등록번호(GeneBank accession No.) NM_017784(oxysterol binding protein-like 10), 유전자등록번호(GeneBank accession No.) NM_017803[dihydrouridine synthase 2-like, SMM1 homolog(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_017854(transmembrane protein 160), 유전자등록번호(GeneBank accession No.) NM_017966[vacuolar protein sorting 37 homolog C(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_018035(hypothetical protein FLJ10241), 유전자등록번호(GeneBank accession No.) NM_018067(MAP7 domain containing 1), 유전자등록번호(GeneBank accession No.) NM_018081(WD repeat domain 79), 유전자등록번호(GeneBank accession No.) NM_018110(docking protein 4), 유전자등록번호(GeneBank accession No.) NM_018111(hypothetical protein FLJ10490), 유전자등록번호(GeneBank accession No.) NM_018125[Rho guanine nucleotide exchange factor(GEF) 10-like], 유전자등록번호(GeneBank accession No.) NM_018150(chromosome 1 open reading frame 164), 유전자등록번호(GeneBank accession No.) NM_018174(microtubule-associated protein 1S), 유전자등록번호(GeneBank accession No.) NM_018226[arginyl aminopeptidase(aminopeptidase B)-like 1], 유전자등록번호(GeneBank accession No.) NM_018231(amino acid transporter), 유전자등록번호(GeneBank accession No.) NM_018358[ATP-binding cassette, sub-family F(GCN20), member 3], 유전자등록번호(GeneBank accession No.) NM_018419[SRY(sex determining region Y)-box 18], 유전자등록번호(GeneBank accession No.) NM_018490(leucine-rich repeat-containing G protein-coupled receptor 4), 유전자등록번호(GeneBank accession No.) NM_018645[hairy and enhancer of split 6(Drosophila)], 유전자등록번 호(GeneBank accession No.) NM_018660(zinc finger protein 395), 유전자등록번호(GeneBank accession No.) NM_018670[mesoderm posterior 1 homolog(mouse)], 유전자등록번호(GeneBank accession No.) NM_018687(hepatocellular carcinoma-associated gene TD26), 유전자등록번호(GeneBank accession No.) NM_018964[solute carrier family 37(glycerol-3-phosphate transporter), member 1], 유전자등록번호(GeneBank accession No.) NM_018973(dolichyl-phosphate mannosyltransferase polypeptide 3), 유전자등록번호(GeneBank accession No.) NM_018979(WNK lysine deficient protein kinase 1), 유전자등록번호(GeneBank accession No.) NM_020158(exosome component 5), 유전자등록번호(GeneBank accession No.) NM_020172(signal peptide peptidase-like 2B), 유전자등록번호(GeneBank accession No.) NM_020210[sema domain, immunoglobulin domain(Ig), transmembrane domain(TM) and short cytoplasmic domain,(semaphorin) 4B], 유전자등록번호(GeneBank accession No.) NM_020246[solute carrier family 12(potassium/chloride transporters), member 9], 유전자등록번호(GeneBank accession No.) NM_020834(homeobox and leucine zipper encoding), 유전자등록번호(GeneBank accession No.) NM_020954(KIAA1618), 유전자등록번호(GeneBank accession No.) NM_021095[solute carrier family 5(sodium-dependent vitamin transporter), member 6], 유전자등록번호(GeneBank accession No.) NM_021170[hairy and enhancer of split 4(Drosophila)], 유전자등록번호(GeneBank accession No.) NM_021247(protamine 3), 유전자등록번호(GeneBank accession No.) NM_021574(breakpoint cluster region), 유전자등록번호(GeneBank accession No.) NM_021603(FXYD domain containing ion transport regulator 2), 유전자등록번호(GeneBank accession No.) NM_021807(exocyst complex component 4), 유전자등록번호(GeneBank accession No.) NM_021932[resistance to inhibitors of cholinesterase 8 homolog A(C. elegans)], 유전자등록번호(GeneBank accession No.) NM_021939(FK506 binding protein 10, 65 kDa), 유전자등록번호(GeneBank accession No.) NM_021969(nuclear receptor subfamily 0, group B, member 2), 유전자등록번호(GeneBank accession No.) NM_022171(T-cell leukemia translocation altered gene), 유전자등록번호(GeneBank accession No.) NM_022372(G protein beta subunit-like), 유전자등록번호(GeneBank accession No.) NM_022449(RAB17, member RAS oncogene family), 유전자등록번호(GeneBank accession No.) NM_022460(HCLS1 binding protein 3), 유전자등록번호(GeneBank accession No.) NM_022833(family with sequence similarity 129, member B), 유전자등록번호(GeneBank accession No.) NM_022908(5'-nucleotidase domain containing 2), 유전자등록번호(GeneBank accession No.) NM_022911(solute carrier family 26, member 6), 유전자등록번호(GeneBank accession No.) NM_022914[adrenocortical dysplasia homolog(mouse)], 유전자등록번호(GeneBank accession No.) NM_023018(NAD kinase), 유전자등록번호(GeneBank accession No.) NM_023939(hypothetical protein MGC2752), 유전자등록번호(GeneBank accession No.) NM_023947(chitinase domain containing 1), 유전자등록번호(GeneBank accession No.) NM_024012[5-hydroxytryptamine(serotonin) receptor 5A], 유전자등록번호(GeneBank accession No.) NM_024066(prion protein interacting protein), 유전자등록번호(GeneBank accession No.) NM_024103[solute carrier family 25(mitochondrial carrier; phosphate carrier), member 23], 유전자등록번호(GeneBank accession No.) NM_024109(chromosome 16 open reading frame 68), 유전자등록번호(GeneBank accession No.) NM_024294(chromosome 6 open reading frame 106), 유전자등록번호(GeneBank accession No.) NM_024296(coiled-coil domain containing 28B), 유전자등록번호(GeneBank accession No.) NM_024310[pleckstrin homology domain containing, family F(with FYVE domain) member 1], 유전자등록번호(GeneBank accession No.) NM_024516(chromosome 16 open reading frame 53), 유전자등록번호(GeneBank accession No.) NM_024536(chondroitin polymerizing factor), 유전자등록번호(GeneBank accession No.) NM_024671(zinc finger protein 768), 유전자등록번호(GeneBank accession No.) NM_024710(isochorismatase domain containing 2), 유전자등록번호(GeneBank accession No.) NM_024827(histone deacetylase 11), 유전자등록번호(GeneBank accession No.) NM_024859(MAGI family member, X-linked), 유전자등록번호(GeneBank accession No.) NM_024872(docking protein 3), 유전자등록번호(GeneBank accession No.) NM_024955(FAD-dependent oxidoreductase domain containing 2), 유전자등록번호(GeneBank accession No.) NM_025021(CREB regulated transcription coactivator 1), 유전자등록번호(GeneBank accession No.) NM_025083[enhancer of mRNA decapping 3 homolog(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_025084(hypothetical protein FLJ22795), 유전자등록번호(GeneBank accession No.) NM_025139(armadillo repeat containing 9), 유전자등록번호(GeneBank accession No.) NM_025207[FAD1 flavin adenine dinucleotide synthetase homolog(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_025227(bactericidal/permeability-increasing protein-like 1), 유전자등록번호(GeneBank accession No.) NM_025233(Coenzyme A synthase), 유전자등록번호(GeneBank accession No.) NM_030578(hypothetical protein MGC4093), 유전자등록번호(GeneBank accession No.) NM_030891(leucine rich repeat containing 3), 유전자등록번호(GeneBank accession No.) NM_030900(transforming growth factor beta regulator 4), 유전자등록번호(GeneBank accession No.) NM_030966(keratin associated protein 1-3), 유전자등록번호(GeneBank accession No.) NM_031209[queuine tRNA-ribosyltransferase 1(tRNA-guanine transglycosylase)], 유전자등록번호(GeneBank accession No.) NM_031232(amyloid beta(A4) precursor protein-binding, family A, member 2 binding protein), 유전자등록번호(GeneBank accession No.) NM_031918(Kruppel-like factor 16), 유전자등록번호(GeneBank accession No.) NM_032125(chromosome 1 open reading frame 160), 유전자등록번호(GeneBank accession No.) NM_032179{Homo sapiens cleavage and polyadenylation specific factor 3-like(CPSF3L), transcript variant 2, mRNA [NM_032179]}, 유전자등록번호(GeneBank accession No.) NM_032271(TNF receptor-associated factor 7), 유전자등록번호(GeneBank accession No.) NM_032283(zinc finger, DHHC-type containing 18), 유전자등록번호(GeneBank accession No.) NM_032326(transmembrane protein 175), 유전자등록번호(GeneBank accession No.) NM_032334(chromosome 8 open reading frame 53), 유전자등록번호(GeneBank accession No.) NM_032349[nudix(nucleoside diphosphate linked moiety X)-type motif 16-like 1], 유전자등록번호(GeneBank accession No.) NM_032389(zinc finger protein 289, ID1 regulated), 유전자등록번호(GeneBank accession No.) NM_032569(cytokine-like nuclear factor n-pac), 유전자등록번호(GeneBank accession No.) NM_032689(zinc finger protein 607), 유전자등록번호(GeneBank accession No.) NM_032789[poly(ADP-ribose) polymerase family, member 10], 유전자등록번호(GeneBank accession No.) NM_032918(RAS-like, estrogen-regulated, growth inhibitor), 유전자등록번호(GeneBank accession No.) NM_032928(transmembrane protein 141), 유전자등록번호(GeneBank accession No.) NM_033036(galactose-3-O-sulfotransferase 3), 유전자등록번호(GeneBank accession No.) NM_033046(rhotekin), 유전자등록번호(GeneBank accession No.) NM_033060(keratin associated protein 4-10), 유전자등록번호(GeneBank accession No.) NM_033178(double homeobox, 4), 유전자등록번호(GeneBank accession No.) NM_033184(keratin associated protein 2-4), 유전자등록번호(GeneBank accession No.) NM_033260(forkhead box Q1), 유전자등록번호(GeneBank accession No.) NM_033310(potassium channel, subfamily K, member 4), 유전자등록번호(GeneBank accession No.) NM_033388[ATG16 autophagy related 16-like 2(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_033453[inosine triphosphatase(nucleoside triphosphate pyrophosphatase)], 유전자등록번호(GeneBank accession No.) NM_033502(transcriptional regulating factor 1), 유전자등록번호(GeneBank accession No.) NM_052853(aarF domain containing kinase 2), 유전자등록번호(GeneBank accession No.) NM_052865(chromosome 20 open reading frame 72), 유전자등록번호(GeneBank accession No.) NM_052880(phosphoinositide-3-kinase interacting protein 1), 유전자등록번호(GeneBank accession No.) NM_052948(sorting nexin 26), 유전자등록번호(GeneBank accession No.) NM_057161(kelch domain containing 3), 유전자등록번호(GeneBank accession No.) NM_058219(exosome component 6), 유전자등록번호(GeneBank accession No.) NM_080686(HLA-B associated transcript 2), 유전자등록번호(GeneBank accession No.) NM_080792(signal-regulatory protein alpha), 유전자등록번호(GeneBank accession No.) NM_080825(chromosome 20 open reading frame 144), 유전자등록번호(GeneBank accession No.) NM_080863(ankyrin repeat and SOCS box-containing 16), 유전자등록번호(GeneBank accession No.) NM_130468[carbohydrate(N-acetylgalactosamine 4-0) sulfotransferase 14], 유전자등록번호(GeneBank accession No.) NM_133177(protein tyrosine phosphatase, receptor type, U), 유전자등록번호(GeneBank accession No.) NM_133371(myozenin 3), 유전자등록번호(GeneBank accession No.) NM_138322(proprotein convertase subtilisin/kexin type 6), 유전자등록번호(GeneBank accession No.) NM_138325(proprotein convertase subtilisin/kexin type 6), 유전자등록번호(GeneBank accession No.) NM_138361(leucine rich repeat and sterile alpha motif containing 1), 유전자등록번호(GeneBank accession No.) NM_138385(transmembrane protein 129), 유전자등록번호(GeneBank accession No.) NM_138387(glucose 6 phosphatase, catalytic, 3), 유전자등록번호(GeneBank accession No.) NM_138412[retinol dehydrogenase 13(all-trans/9-cis)], 유전자등록번호(GeneBank accession No.) NM_138578(BCL2-like 1), 유전자등록번호(GeneBank accession No.) NM_138763(BCL2-associated X protein), 유전자등록번호(GeneBank accession No.) NM_139067(SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2), 유전자등록번호(GeneBank accession No.) NM_139280[ORM1-like 3(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_145040(protein kinase C, delta binding protein), 유전자등록번호(GeneBank accession No.) NM_145110{Homo sapiens mitogen-activated protein kinase kinase 3(MAP2K3), transcript variant C, mRNA [NM_145110]}, 유전자등록번호(GeneBank accession No.) NM_145179{Homo sapiens chromosome 21 open reading frame 93(C21orf93), mRNA [NM_145179]}, 유전자등록번호(GeneBank accession No.) NM_145343(apolipoprotein L, 1), 유전자등록번호(GeneBank accession No.) NM_145345(UBX domain containing 5), 유전자등록번호(GeneBank accession No.) NM_145685[BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_145870[glutathione transferase zeta 1(maleylacetoacetate isomerase)], 유전자등록번호(GeneBank accession No.) NM_145904(high mobility group AT-hook 1), 유전자등록번호(GeneBank accession No.) NM_147780(cathepsin B), 유전자등록번호(GeneBank accession No.) NM_152243[CDC42 effector protein(Rho GTPase binding) 1], 유전자등록번호(GeneBank accession No.) NM_152272(CHMP family, member 7), 유전자등록번호(GeneBank accession No.) NM_152299(non-SMC condensin II complex, subunit H2), 유전자등록번호(GeneBank accession No.) NM_152326(ankyrin repeat domain 9), 유전자등록번호(GeneBank accession No.) NM_152600(zinc finger protein 579), 유전자등록번호(GeneBank accession No.) NM_153002(G protein-coupled receptor 156), 유전자등록번호(GeneBank accession No.) NM_153200(endothelial differentiation-related factor 1), 유전자등록번호(GeneBank accession No.) NM_153236(GTPase, IMAP family member 7), 유전자등록번호(GeneBank accession No.) NM_153253(signal-induced proliferation-associated gene 1), 유전자등록번호(GeneBank accession No.) NM_153284(hyaluronoglucosaminidase 1), 유전자등록번호(GeneBank accession No.) NM_153320[solute carrier family 22(organic anion transporter), member 7], 유전자등록번호(GeneBank accession No.) NM_153461(interleukin 17 receptor C), 유전자등록번호(GeneBank accession No.) NM_153631(homeobox A3), 유전자등록번호(GeneBank accession No.) NM_153824(pyrroline-5-carboxylate reductase 1), 유전자등록번호(GeneBank accession No.) NM_153827[misshapen-like kinase 1(zebrafish)], 유전자등록번호(GeneBank accession No.) NM_170692(RAS protein activator like 2), 유전자등록번호(GeneBank accession No.) NM_170726(aldehyde dehydrogenase 4 family, member A1), 유전자등록번호(GeneBank accession No.) NM_171982(tripartite motif-containing 35), 유전자등록번호(GeneBank accession No.) NM_172027[ankyrin repeat and BTB(POZ) domain containing 1], 유전자등록번호(GeneBank accession No.) NM_172171[calcium/calmodulin-dependent protein kinase(CaM kinase) II gamma], 유전자등록번호(GeneBank accession No.) NM_173179(solute carrier family 35, member C2), 유전자등록번호(GeneBank accession No.) NM_173354(SNF1-like kinase), 유전자등록번호(GeneBank accession No.) NM_173467[malonyl CoA:ACP acyltransferase(mitochondrial)], 유전자등록번호(GeneBank accession No.) NM_173546(kelch domain containing 8B), 유전자등록번호(GeneBank accession No.) NM_173596[solute carrier family 39(metal ion transporter), member 5], 유전자등록번호(GeneBank accession No.) NM_173687(chromosome 8 open reading frame 31), 유전자등록번호(GeneBank accession No.) NM_173728[Rho guanine nucleotide exchange factor(GEF) 15], 유전자등록번호(GeneBank accession No.) NM_174903(ring finger protein 151), 유전자등록번호(GeneBank accession No.) NM_174910(t-complex-associated-testis-expressed 3), 유전자등록번호(GeneBank accession No.) NM_174913(chromosome 14 open reading frame 21), 유전자등록번호(GeneBank accession No.) NM_174937(transcription elongation regulator 1-like), 유전자등록번호(GeneBank accession No.) NM_174983(chromosome 19 open reading frame 28), 유전자등록번호(GeneBank accession No.) NM_175609(ADP-ribosylation factor GTPase activating protein 1), 유전자등록번호(GeneBank accession No.) NM_175709(chromobox homolog 7), 유전자등록번호(GeneBank accession No.) NM_175734(chromosome 17 open reading frame 74), 유전자등록번호(GeneBank accession No.) NM_175852(taxilin alpha), 유전자등록번호(GeneBank accession No.) NM_177967(UBA domain containing 2), 유전자등록번호(GeneBank accession No.) NM_177987(tubulin, beta 8), 유전자등록번호(GeneBank accession No.) NM_178150(F-box protein, helicase, 18), 유전자등록번호(GeneBank accession No.) NM_178176(monoacylglycerol O-acyltransferase 3), 유전자등록번호(GeneBank accession No.) NM_178326[ATG4 autophagy related 4 homolog B(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_178352(late cornified envelope 1D), 유전자등록번호(GeneBank accession No.) NM_178433(late cornified envelope 3B), 유전자등록번호(GeneBank accession No.) NM_178518(transmembrane protein 102), 유전자등록번호(GeneBank accession No.) NM_178519(chromosome 17 open reading frame 55), 유전자등록번호(GeneBank accession No.) NM_178819[1-acylglycerol-3-phosphate O-acyltransferase 6(lysophosphatidic acid acyltransferase, zeta)], 유전자등록번호(GeneBank accession No.) NM_178830(chromosome 19 open reading frame 47), 유전자등록번호(GeneBank accession No.) NM_181468(integrin beta 4 binding protein), 유전자등록번호(GeneBank accession No.) NM_181718(aspartate beta-hydroxylase domain containing 1), 유전자등록번호(GeneBank accession No.) NM_181738(peroxiredoxin 2), 유전자등록번호(GeneBank accession No.) NM_181809(bone morphogenetic protein 8a), 유전자등록번호(GeneBank accession No.) NM_182470(pyruvate kinase, muscle), 유전자등록번호(GeneBank accession No.) NM_182557(B-cell CLL/lymphoma 9-like), 유전자등록번호(GeneBank accession No.) NM_182614(family with sequence similarity 70, member B), 유전자등록번호(GeneBank accession No.) NM_182947(RAC/CDC42 exchange factor), 유전자등록번호(GeneBank accession No.) NM_182983[hepsin(transmembrane protease, serine 1)], 유전자등록번호(GeneBank accession No.) NM_183352[SEC13 homolog(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_184041(aldolase A, fructose-bisphosphate), 유전자등록번호(GeneBank accession No.) NM_194278(chromosome 14 open reading frame 43), 유전자등록번호(GeneBank accession No.) NM_194302(coiled-coil domain containing 108), 유전자등록번호(GeneBank accession No.) NM_198040[polyhomeotic homolog 2(Drosophila)], 유전자등록번호(GeneBank accession No.) NM_198061[carboxylesterase 2(intestine, liver)], 유전자등록번호(GeneBank accession No.) NM_198149(transmembrane protein 58), 유전자등록번호(GeneBank accession No.) NM_198181(hypothetical protein LOC440295), 유전자등록번호(GeneBank accession No.) NM_198284{Homo sapiens hypothetical protein LOC349114(LOC349114), mRNA [NM_198284]}, 유전자등록번호(GeneBank accession No.) NM_198334(glucosidase, alpha; neutral AB), 유전자등록번호(GeneBank accession No.) NM_199037(sodium channel, voltage-gated, type I, beta), 유전자등록번호(GeneBank accession No.) NM_199345(similar to phosphatidylinositol 4-kinase alpha), 유전자등록번호(GeneBank accession No.) NM_201382(plectin 1, intermediate filament binding protein 500kDa), 유전자등록번호(GeneBank accession No.) NM_201535(NDRG family member 2), 유전자등록번호(GeneBank accession No.) NM_201629[tight junction protein 2(zona occludens 2)], 유전자등록번호(GeneBank accession No.) NM_203344(SERTA domain containing 3), 유전자등록번호(GeneBank accession No.) NM_203351(mitogen-activated protein kinase kinase kinase 3), 유전자등록번호(GeneBank accession No.) NM_203393(hypothetical gene supported by BC031661), 유전자등록번호(GeneBank accession No.) NM_206949(family with sequence similarity 14, member B), 유전자등록번호(GeneBank accession No.) NM_206962(protein arginine methyltransferase 2), 유전자등록번호(GeneBank accession No.) NM_207107(UCHL5 interacting protein), 유전자등록번호(GeneBank accession No.) NM_207370(G protein-coupled receptor 153), 유전자등록번호(GeneBank accession No.) NM_213604(ADAMTS-like 5), 유전자등록번호(GeneBank accession No.) NM_213647(fibroblast growth factor receptor 4), 유전자등록번호(GeneBank accession No.) NM_214710(protease, serine-like 1), 유전자등록번 호(GeneBank accession No.) NP106190{GB|X76785.1|CAA54180.1 hypothetical protein [NP106190]}, 유전자등록번호(GeneBank accession No.) NR_002187(hypothetical protein LOC286016), 유전자등록번호(GeneBank accession No.) NR_002603(nuclear pore complex interacting protein pseudogene), 유전자등록번호(GeneBank accession No.) NR_002797(hypothetical protein LOC255783), 유전자등록번호(GeneBank accession No.) NR_002837(ubiquitin-conjugating enzyme E2M pseudogene 1), 유전자등록번호(GeneBank accession No.) NR_002929(actin pseudogene), 유전자등록번호(GeneBank accession No.) S72422[dihydrolipoamide S-succinyltransferase pseudogene(E2 component of 2-oxo-glutarate complex)], 유전자등록번호(GeneBank accession No.) U21012[Clone 120Pa immunoglobulin light chain variable region(VkJ)], 유전자등록번호(GeneBank accession No.) U35612[SRY(sex determining region Y)-box 12], 유전자등록번호(GeneBank accession No.) U63828(chromosome 20 open reading frame 181), 유전자등록번호(GeneBank accession No.) U68019(SMAD family member 3), 유전자등록번호(GeneBank accession No.) XM_028217(hypothetical LOC90024), 유전자등록번호(GeneBank accession No.) XM_293937{PREDICTED: Homo sapiens similar to ankyrin repeat domain 33(LOC345711), mRNA [XM_293937]}, 유전자등록번호(GeneBank accession No.) XM_373788{full-length cDNA clone CS0DF014YD20 of Fetal brain of Homo sapiens(human). [CR593500]}, 유전자등록번호(GeneBank accession No.) XM_926717(similar to actin, alpha 2, smooth muscle, aorta), 유 전자등록번호(GeneBank accession No.) XM_927514[similar to Elongation factor 1-delta(EF-1-delta)(Antigen NY-CO-4)], 유전자등록번호(GeneBank accession No.) XM_928285{PREDICTED: Homo sapiens similar to protein expressed in prostate, ovary, testis, and placenta 15, transcript variant 1(LOC653239), mRNA [XM_928285]}, 유전자등록번호(GeneBank accession No.) XM_928640{Homo sapiens N-deacetylase/N-sulfotransferase(heparan glucosaminyl) 2, mRNA(cDNA clone IMAGE:4665687), partial cds. [BC018681]}, 유전자등록번호(GeneBank accession No.) XM_932013{PREDICTED: Homo sapiens hypothetical protein LOC644134(LOC644134), mRNA [XM_932013]}, 유전자등록번호(GeneBank accession No.) XM_936535{PREDICTED: Homo sapiens hypothetical protein LOC648245, transcript variant 1(LOC648245), mRNA [XM_936535]}, 유전자등록번호(GeneBank accession No.) XM_937698(similar to synaptogyrin 2), 유전자등록번호(GeneBank accession No.) XM_943677{Homo sapiens hypothetical LOC389634, mRNA(cDNA clone IMAGE:4157715). [BC037255]}, 유전자등록번호(GeneBank accession No.) XM_946056{PREDICTED: Homo sapiens hypothetical protein LOC643669, transcript variant 2(LOC643669), mRNA [XM_933624]}.Gene access number (GeneBank accession No. ) AF011794 (cell cycle progression 1), Gene accession number (GeneBank accession No. AF118092 (predicted protein of HQ2061; Homo sapiens PRO2061 mRNA, complete cds. ), Gene accession number (GeneBank accession No. AF119870 (predicted protein of HQ2266; Homo sapiens PRO2266 mRNA, complete cds. ), Gene accession number (GeneBank accession No. ) AF274942 (PNAS-17), Gene Accession Number (GeneBank accession No. ) AI382322 (Collagen, type XXVII, alpha 1), Gene accession number (GeneBank accession No. ) AI570094 [Transcribed locus, weakly similar to NP_033865. 2 (Mus musculus)], GeneBank accession No. ) AI760454 [Hairy and enhancer of split 2 (Drosophila)], gene accession number (GeneBank accession No. ) AK054921 (Cerebellar degeneration-
1) 상기 바이오마커 중에서 헥사클로로벤젠의 노출에 의해 발현이 증가하는 바이오마커 유전자는 하기와 같다:1) Biomarker genes whose expression is increased by exposure of hexachlorobenzene in the biomarkers are as follows:
유전자등록번호(GeneBank accession No.) AF011794(cell cycle progression 1), 유전자등록번호(GeneBank accession No.) AF118092(predicted protein of HQ2061; Homo sapiens PRO2061 mRNA, complete cds.), 유전자등록번호(GeneBank accession No.) AF119870(predicted protein of HQ2266; Homo sapiens PRO2266 mRNA, complete cds.), 유전자등록번호(GeneBank accession No.) AF274942(PNAS-17), 유전자등록번호(GeneBank accession No.) AI382322(Collagen, type XXVII, alpha 1), 유전자등록번호(GeneBank accession No.) AI570094[Transcribed locus, weakly similar to NP_033865.2 (Mus musculus)], 유전자등록번호(GeneBank accession No.) AI760454[Hairy and enhancer of split 2(Drosophila)], 유전자등록번호(GeneBank accession No.) AK054921(Cerebellar degeneration-related protein 1, 34kDa), 유전자등록번호(GeneBank accession No.) AK128263(Hypothetical protein MGC15523), 유전자등록번호(GeneBank accession No.) AK128714(Similar to embigin homolog), 유전자등록번호(GeneBank accession No.) BC006406(family with sequence similarity 104, member B), 유전자등록번호(GeneBank accession No.) BC009297(hypothetical protein FLJ22222), 유전자등록번호(GeneBank accession No.) BC012091[hairy and enhancer of split 2(Drosophila)], 유전자등록번호(GeneBank accession No.) BC032824[Homo sapiens chromosome 6 open reading frame 164, mRNA(cDNA clone IMAGE:4824064).], 유전자등록번호(GeneBank accession No.) BC045756(glycoprotein, alpha-galactosyltransferase 1), 유전자등록번호(GeneBank accession No.) CR615245[Similar to 40S ribosomal protein S3a(V-fos transformation effector protein)], 유전자등록번호(GeneBank accession No.) CR621148[Interleukin 6 signal transducer(gp130, oncostatin M receptor)], 유전자등록번호(GeneBank accession No.) CR627148(Homo sapiens, clone IMAGE:5213378, mRNA), 유전자등록번호(GeneBank accession No.) NM_000014(alpha-2-macroglobulin), 유전자등록번호(GeneBank accession No.) NM_000042[apolipoprotein H(beta-2-glycoprotein I)], 유전자등록번호(GeneBank accession No.) NM_000043[Fas(TNF receptor superfamily, member 6)], 유전자등록번호(GeneBank accession No.) NM_000130[coagulation factor V(proaccelerin, labile factor)], 유전자등록번호(GeneBank accession No.) NM_000242[mannose-binding lectin(protein C) 2, soluble(opsonic defect)], 유전자등록번호(GeneBank accession No.) NM_000301(plasminogen), 유전자등록번호(GeneBank accession No.) NM_000509(fibrinogen gamma chain), 유전자등록번호(GeneBank accession No.) NM_000582[secreted phosphoprotein 1(osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)], 유전자등록번호(GeneBank accession No.) NM_000633(B-cell CLL/lymphoma 2), 유전자등록번호(GeneBank accession No.) NM_000761(cytochrome P450, family 1, subfamily A, polypeptide 2), 유전자등록번호(GeneBank accession No.) NM_000846(glutathione S-transferase A2), 유전자등록번호(GeneBank accession No.) NM_000847(glutathione S-transferase A3), 유전자등록번호(GeneBank accession No.) NM_000859(3-hydroxy-3-methylglutaryl-Coenzyme A reductase), 유전자등록번호(GeneBank accession No.) NM_000997(ribosomal protein L37), 유전자등록번호(GeneBank accession No.) NM_001002236(serpin peptidase inhibitor, clade A(alpha-1 antiproteinase, antitrypsin), member 1), 유전자등록번호(GeneBank accession No.) NM_001014(ribosomal protein S10), 유전자등록번호(GeneBank accession No.) NM_001017(ribosomal protein S13), 유전자등록번호(GeneBank accession No.) NM_001072(UDP glucuronosyltransferase 1 family, polypeptide A6), 유전자등록번호(GeneBank accession No.) NM_001085[serpin peptidase inhibitor, clade A(alpha-1 antiproteinase, antitrypsin), member 3], 유전자등록번호(GeneBank accession No.) NM_001134(alpha-fetoprotein), 유전자등록번호(GeneBank accession No.) NM_001402(eukaryotic translation elongation factor 1 alpha 1), 유전자등록번호(GeneBank accession No.) NM_001443(fatty acid binding protein 1, liver), 유전자등록번호(GeneBank accession No.) NM_001568(eukaryotic translation initiation factor 3, subunit 6 48kDa), 유전자등록번호(GeneBank accession No.) NM_001793[cadherin 3, type 1, P-cadherin(placental)], 유전자등록번호(GeneBank accession No.) NM_001932[membrane protein, palmitoylated 3(MAGUK p55 subfamily member 3)], 유전자등록번호(GeneBank accession No.) NM_002130[3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1(soluble)], 유전자등록번호(GeneBank accession No.) NM_002210[integrin, alpha V(vitronectin receptor, alpha polypeptide, antigen CD51)], 유전자등록번호(GeneBank accession No.) NM_002567(phosphatidylethanolamine binding protein 1), 유전자 등록번호(GeneBank accession No.) NM_002864(pregnancy-zone protein), 유전자등록번호(GeneBank accession No.) NM_003053[solute carrier family 18(vesicular monoamine), member 1], 유전자등록번호(GeneBank accession No.) NM_003126[spectrin, alpha, erythrocytic 1(elliptocytosis 2)], 유전자등록번호(GeneBank accession No.) NM_003262(translocation protein 1), 유전자등록번호(GeneBank accession No.) NM_003728[unc-5 homolog C(C. elegans)], 유전자등록번호(GeneBank accession No.) NM_003803(myomesin 1, 185kDa), 유전자등록번호(GeneBank accession No.) NM_003936[cyclin-dependent kinase 5, regulatory subunit 2(p39)], 유전자등록번호(GeneBank accession No.) NM_003947(kalirin, RhoGEF kinase), 유전자등록번호(GeneBank accession No.) NM_004048(beta-2-microglobulin), 유전자등록번호(GeneBank accession No.) NM_004066(centrin, EF-hand protein, 1), 유전자등록번호(GeneBank accession No.) NM_004164(retinol binding protein 2, cellular), 유전자등록번호(GeneBank accession No.) NM_004378(cellular retinoic acid binding protein 1), 유전자등록번호(GeneBank accession No.) NM_004508(isopentenyl-diphosphate delta isomerase 1), 유전자등록번호(GeneBank accession No.) NM_004821(heart and neural crest derivatives expressed 1), 유전자등록번호(GeneBank accession No.) NM_005127(C-type lectin domain family 2, member B), 유전자등록번호(GeneBank accession No.) NM_005348[heat shock protein 90kDa alpha(cytosolic), class A member 1], 유전자등록번호(GeneBank accession No.) NM_005408[chemokine(C-C motif) ligand 13], 유전자등록번호(GeneBank accession No.) NM_005602[claudin 11(oligodendrocyte transmembrane protein)], 유전자등록번호(GeneBank accession No.) NM_006121[keratin 1(epidermolytic hyperkeratosis)], 유전자등록번호(GeneBank accession No.) NM_006136[capping protein(actin filament) muscle Z-line, alpha 2], 유전자등록번호(GeneBank accession No.) NM_006398(ubiquitin D), 유전자등록번호(GeneBank accession No.) NM_012135(family with sequence similarity 50, member B), 유전자등록번호(GeneBank accession No.) NM_012198(grancalcin, EF-hand calcium binding protein), 유전자등록번호(GeneBank accession No.) NM_014332(small muscle protein, X-linked), 유전자등록번호(GeneBank accession No.) NM_014619(glutamate receptor, ionotropic, kainate 4), 유전자등록번호(GeneBank accession No.) NM_016649[ESF1, nucleolar pre-rRNA processing protein, homolog(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_017493(OTU domain containing 4), 유전자등록번호(GeneBank accession No.) NM_017826(spermatogenesis and oogenesis specific basic helix-loop-helix 2), 유전자등록번호(GeneBank accession No.) NM_017855(odontogenic, ameloblast asssociated), 유전자등록번호(GeneBank accession No.) NM_019000(family with sequence similarity 134, member B), 유전자등록번호(GeneBank accession No.) NM_020805[kelch-like 14(Drosophila)], 유전자등록번호(GeneBank accession No.) NM_021009(ubiquitin C), 유전자등록번호(GeneBank accession No.) NM_021104(ribosomal protein L41), 유전자등록번호(GeneBank accession No.) NM_021631(apoptosis inhibitor), 유전자등록번호(GeneBank accession No.) NM_024665[transducin(beta)-like 1X-linked receptor 1], 유전자등록번호(GeneBank accession No.) NM_031308(epiplakin 1), 유전자등록번호(GeneBank accession No.) NM_053275(ribosomal protein, large, P0), 유전자등록번호(GeneBank accession No.) NM_138409(chromosome 6 open reading frame 117), 유전자등록번호(GeneBank accession No.) NM_139137(potassium voltage-gated channel, Shaw-related subfamily, member 2), 유전자등록번호(GeneBank accession No.) NM_145740(glutathione S-transferase A1), 유전자등록번호(GeneBank accession No.) NM_153699(glutathione S-transferase A5), 유전자등록번호(GeneBank accession No.) NM_153811(solute carrier family 38, member 6), 유전자등록번호(GeneBank accession No.) NM_173568(uromodulin-like 1), 유전자등록번호(GeneBank accession No.) NM_181054[hypoxia-inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor)], 유전자등록번호(GeneBank accession No.) NM_203304(ring finger and KH domain containing 1), 유전자등록번호(GeneBank accession No.) XM_498623{PREDICTED: Homo sapiens hypothetical LOC440309(LOC440309), mRNA [XM_498623]}, 유전자등록번호(GeneBank accession No.) XM_928021{Homo sapiens clone DNA147258 LMNE6487(UNQ6487) mRNA, complete cds. [AY358240]}, 유전자등록번호(GeneBank accession No.) XM_931362{PREDICTED: Homo sapiens similar to ribosomal protein S3a, transcript variant 3(LOC439992), mRNA [XM_931362]}, 유전자등록번호(GeneBank accession No.) XM_935867{Homo sapiens cDNA clone IMAGE:3621376, partial cds. [BC012751]}, 유전자등록번호(GeneBank accession No.) XM_940931(similar to fibrillarin).GeneBank accession No.AF011794 (cell cycle progression 1), GeneBank accession No.AF118092 (predicted protein of HQ2061; Homo sapiens PRO2061 mRNA, complete cds.), GeneBank accession No. AF119870 (predicted protein of HQ2266; Homo sapiens PRO2266 mRNA, complete cds.), GeneBank accession No. AF274942 (PNAS-17), GeneBank accession No. AI382322 (Collagen, type XXVII) , alpha 1), GeneBank accession No. AI570094 [Transcribed locus, weakly similar to NP_033865.2 (Mus musculus)], GeneBank accession No. AI760454 [Hairy and enhancer of split 2 (Drosophila) )], GeneBank accession No. AK054921 (Cerebellar degeneration-related
2) 상기 바이오마커 중에서, 헥사클로로벤젠의 노출에 의해 발현이 감소하는 바이오마커 유전자는 하기와 같다:2) Among the biomarkers, biomarker genes whose expression is reduced by exposure of hexachlorobenzene are as follows:
유전자등록번호(GeneBank accession No.) AA449398(castor zinc finger 1), 유전자등록번호(GeneBank accession No.) AB040942(coiled-coil domain containing 88C), 유전자등록번호(GeneBank accession No.) AB065089(ribosomal protein S2), 유전자등록번호(GeneBank accession No.) AF005082(chromosome 1 open reading frame 46), 유전자등록번호(GeneBank accession No.) AF090927(Homo sapiens clone HQ0457 PRO0457 mRNA, complete cds.), 유전자등록번호(GeneBank accession No.) AF113013(HQ0806; Homo sapiens PRO0806 mRNA, complete cds.), 유전자등록번호(GeneBank accession No.) AF131778[discs, large(Drosophila) homolog-associated protein 3], 유전자등록번호(GeneBank accession No.) AF318368(hypothetical protein LOC650662), 유전자등록번호(GeneBank accession No.) AF323119[nuclear factor(erythroid-derived 2)-like 2], 유전자등록번호(GeneBank accession No.) AF357221(FKSG88), 유전자등록번호(GeneBank accession No.) AI500335[Bruno-like 5, RNA binding protein(Drosophila)], 유전자등록번호(GeneBank accession No.) AI801539{Transcribed locus, moderately similar to XP_001109274.1 heat shock 27kDa protein 1 [Macaca mulatta]}, 유전자등록번호(GeneBank accession No.) AK001891(proline rich 11), 유전자등록번호(GeneBank accession No.) AK002097(hypothetical protein FLJ11235), 유전자등록번호(GeneBank accession No.) AK021860(F-box protein 17), 유전자등록번호(GeneBank accession No.) AK054771(v-akt murine thymoma viral oncogene homolog 2), 유전자등록번호(GeneBank accession No.) AK057432(IQ motif containing F3), 유전자등록번호(GeneBank accession No.) AK074121(platelet endothelial aggregation receptor 1), 유전자등록번호(GeneBank accession No.) AK074175[leucine-rich repeats and calponin homology(CH) domain containing 4], 유전자등록번호(GeneBank accession No.) AK074778(hypothetical protein FLJ90297), 유전자등록번호(GeneBank accession No.) AK092572(hypothetical protein LOC148413), 유전자등록번호(GeneBank accession No.) AK093729(Hypothetical gene supported by AK127273), 유전자등록번호(GeneBank accession No.) AK095727(CDNA FLJ38408 fis, clone FEBRA2009029), 유전자등록번호(GeneBank accession No.) AK095933(chromodomain helicase DNA binding protein 2), 유전자등록번호(GeneBank accession No.) AK096031(CDNA FLJ38712 fis, clone KIDNE2006733), 유전자등록번호(GeneBank accession No.) AK096643(hypothetical protein FLJ22184), 유전자등록번호(GeneBank accession No.) AK097259(Docking protein 3), 유전자등록번호(GeneBank accession No.) AK098372(Neuroblastoma breakpoint family, member 1), 유전자등록번호(GeneBank accession No.) AK123855(chromosome 1 open reading frame 170), 유전자등록번호(GeneBank accession No.) AK126622(WD repeat domain 90), 유전자등록번호(GeneBank accession No.) AK127768[MRNA; cDNA DKFZp547C1918(from clone DKFZp547C1918)], 유전자등록번호(GeneBank accession No.) AK128072(CDNA FLJ46193 fis, clone TESTI4006234), 유전자등록번호(GeneBank accession No.) AK129584[Full-length cDNA clone CS0DI007YH13 of Placenta Cot 25-normalized of Homo sapiens(human)], 유전자등록번호(GeneBank accession No.) AL050297(R3H domain and coiled-coil containing 1), 유전자등록번호(GeneBank accession No.) AL136837(hypothetical protein DKFZp434F142), 유전자등록번호(GeneBank accession No.) AL564305(Clusterin), 유전자등록번호(GeneBank accession No.) AL566332(Sorting nexin 26), 유전자등록번호(GeneBank accession No.) AX775899(unnamed protein product; Sequence 169 from Patent WO03048202.), 유전자등록번호(GeneBank accession No.) AY945262{Homo sapiens mucin(MUC8) mRNA, complete cds. [AY945262]}, 유전자등록번호(GeneBank accession No.) BC009872(branched chain ketoacid dehydrogenase kinase), 유전자등록번호(GeneBank accession No.) BC014228(prohibitin pseudogene), 유전자등록번호(GeneBank accession No.) BC014606(CDNA clone IMAGE:4826808), 유전자등록번호(GeneBank accession No.) BC016140[mevalonate kinase(mevalonic aciduria)], 유전자등록번호(GeneBank accession No.) BC018035(CDNA clone IMAGE:4819956), 유전자등록번호(GeneBank accession No.) BC021118(paroxysmal nonkinesiogenic dyskinesia), 유전자등록번호(GeneBank accession No.) BC022394(zinc finger protein 493), 유전자등록번호(GeneBank accession No.) BC022562(CDNA clone IMAGE:4794059), 유전자등록번호(GeneBank accession No.) BC025310(leucine rich repeat and fibronectin type III domain containing 1), 유전자등록번호(GeneBank accession No.) BC027988(hypothetical protein LOC253842), 유전자등록번호(GeneBank accession No.) BC033110[hydroxysteroid(17-beta) dehydrogenase 1], 유전자등록번호(GeneBank accession No.) BC042034(chromosome 7 open reading frame 46), 유전자등록번호(GeneBank accession No.) BC062790(hypothetical gene supported by BC038466; BC062790), 유전자등록번호(GeneBank accession No.) BC063316(F-box and leucine-rich repeat protein 17), 유전자등록번호(GeneBank accession No.) BC080624(Similar to Rho GTPase activating protein 15), 유전자등록번호(GeneBank accession No.) BC092421(Hypothetical gene supported by AK127273), 유전자등록번호(GeneBank accession No.) BC106086(chromosome 20 open reading frame 194), 유전자등록번호(GeneBank accession No.) BC110642(p20), 유전자등록번호(GeneBank accession No.) BG259069(Transcribed locus), 유전자등록번호(GeneBank accession No.) BG620990(Similar to ATP binding domain 3), 유전자등록번호(GeneBank accession No.) BM054818(Mitochondrial ribosomal protein S26), 유전자등록번호(GeneBank accession No.) BU633383(UI-H-FL1-bgu-h-03-0-UI.s1 NCI_CGAP_FL1 Homo sapiens cDNA clone UI-H-FL1-bgu-h-03-0-UI 3', mRNA sequence.), 유전자등록번호(GeneBank accession No.) BX104999(Transcribed locus), 유전자등록번호(GeneBank accession No.) BX111592(Transcribed locus), 유전자등록번호(GeneBank accession No.) BX648950[MRNA; cDNA DKFZp686E0389(from clone DKFZp686E0389)], 유전자등록번호(GeneBank accession No.) CR600494(Hypothetical protein BC006130), 유전자등록번호(GeneBank accession No.) CR601391(Chromosome 1 open reading frame 144), 유전자등록번호(GeneBank accession No.) CR609146[ATP-binding cassette, sub-family C(CFTR/MRP), member 6], 유전자등록번호(GeneBank accession No.) CR936831(C8orfK32 protein), 유전자등록번호(GeneBank accession No.) CV402334[Similar to Adenosylhomocysteinase(S-adenosyl-L-homocysteine hydrolase)(AdoHcyase)], 유전자등록번호(GeneBank accession No.) DA072538(Transcribed locus), 유전자등록번호(GeneBank accession No.) J05272[IMP(inosine monophosphate) dehydrogenase 1], 유전자등록번호(GeneBank accession No.) M94173(calcium channel, voltage-dependent, N type, alpha 1B subunit), 유전자등록번호(GeneBank accession No.) NM_000189(hexokinase 2), 유전자등록번호(GeneBank accession No.) NM_000195(Hermansky-Pudlak syndrome 1), 유전자등록번호(GeneBank accession No.) NM_000221[ketohexokinase(fructokinase)], 유전자등록번호(GeneBank accession No.) NM_000229(lecithin-cholesterol acyltransferase), 유전자등록번호(GeneBank accession No.) NM_000263[N-acetylglucosaminidase, alpha-(Sanfilippo disease IIIB)], 유전자등록번호(GeneBank accession No.) NM_000302(procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1), 유전자등록번 호(GeneBank accession No.) NM_000303(phosphomannomutase 2), 유전자등록번호(GeneBank accession No.) NM_000403(UDP-galactose-4-epimerase), 유전자등록번호(GeneBank accession No.) NM_000457(hepatocyte nuclear factor 4, alpha), 유전자등록번호(GeneBank accession No.) NM_000481(aminomethyltransferase), 유전자등록번호(GeneBank accession No.) NM_000495[collagen, type IV, alpha 5(Alport syndrome)], 유전자등록번호(GeneBank accession No.) NM_000594[tumor necrosis factor(TNF superfamily, member 2)], 유전자등록번호(GeneBank accession No.) NM_000638(vitronectin), 유전자등록번호(GeneBank accession No.) NM_000709(branched chain keto acid dehydrogenase E1, alpha polypeptide), 유전자등록번호(GeneBank accession No.) NM_000713{biliverdin reductase B [flavin reductase(NADPH)]}, 유전자등록번호(GeneBank accession No.) NM_000789[angiotensin I converting enzyme(peptidyl-dipeptidase A) 1], 유전자등록번호(GeneBank accession No.) NM_000841(glutamate receptor, metabotropic 4), 유전자등록번호(GeneBank accession No.) NM_000876(insulin-like growth factor 2 receptor), 유전자등록번호(GeneBank accession No.) NM_000918[procollagen-proline, 2-oxoglutarate 4-dioxygenase(proline 4-hydroxylase), beta polypeptide], 유전자등록번호(GeneBank accession No.) NM_000923[phosphodiesterase 4C, cAMP-specific(phosphodiesterase E1 dunce homolog, Drosophila)], 유전자등록번호(GeneBank accession No.) NM_000991(ribosomal protein L28), 유전자등록번호(GeneBank accession No.) NM_001001290[solute carrier family 2(facilitated glucose transporter), member 9], 유전자등록번호(GeneBank accession No.) NM_001001975(ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit), 유전자등록번호(GeneBank accession No.) NM_001004313(similar to RIKEN cDNA A730055C05 gene), 유전자등록번호(GeneBank accession No.) NM_001004349(FLJ45422 protein), 유전자등록번호(GeneBank accession No.) NM_001004356(fibroblast growth factor receptor-like 1), 유전자등록번호(GeneBank accession No.) NM_001005209(similar to RIKEN cDNA A230078I05 gene), 유전자등록번호(GeneBank accession No.) NM_001007125(bA299N6.3), 유전자등록번호(GeneBank accession No.) NM_001008397(similar to RIKEN cDNA 2310016C16), 유전자등록번호(GeneBank accession No.) NM_001013(ribosomal protein S9), 유전자등록번호(GeneBank accession No.) NM_001013839(exocyst complex component 7), 유전자등록번호(GeneBank accession No.) NM_001014763(electron-transfer-flavoprotein, beta polypeptide), 유전자등록번호(GeneBank accession No.) NM_001014765(F-box protein 44), 유전자등록번호(GeneBank accession No.) NM_001017418(small proline-rich protein 2B), 유전자등록번호(GeneBank accession No.) NM_001025200(chymotrypsinogen B2), 유전자등록번호(GeneBank accession No.) NM_001033515(similar to hypothetical protein MGC27019), 유전자등록번호(GeneBank accession No.) NM_001035235(steroid receptor RNA activator 1), 유전자등록번호(GeneBank accession No.) NM_001037125[unkempt homolog(Drosophila)-like], 유전자등록번호(GeneBank accession No.) NM_001039464(chromosome 1 open reading frame 175), 유전자등록번호(GeneBank accession No.) NM_001039476(chromosome 16 open reading frame 35), 유전자등록번호(GeneBank accession No.) NM_001039842(hypothetical protein LOC339229), 유전자등록번호(GeneBank accession No.) NM_001040078(similar to galectin 9 short isoform), 유전자등록번호(GeneBank accession No.) NM_001064[transketolase(Wernicke-Korsakoff syndrome)], 유전자등록번호(GeneBank accession No.) NM_001070(tubulin, gamma 1), 유전자등록번호(GeneBank accession No.) NM_001087(angio-associated, migratory cell protein), 유전자등록번호(GeneBank accession No.) NM_001098(aconitase 2, mitochondrial), 유전자등록번호(GeneBank accession No.) NM_001102(actinin, alpha 1), 유전자등록번호(GeneBank accession No.) NM_001106(activin A receptor, type IIB), 유전자등록번호(GeneBank accession No.) NM_001152[solute carrier family 25(mitochondrial carrier; adenine nucleotide translocator), member 5], 유전자등록번호(GeneBank accession No.) NM_001155(annexin A6), 유전자등록번호(GeneBank accession No.) NM_001188(BCL2-antagonist/killer 1), 유전자등록번호(GeneBank accession No.) NM_001191(BCL2-like 1), 유전자등록번호(GeneBank accession No.) NM_001250(CD40 molecule, TNF receptor superfamily member 5), 유전자등록번호(GeneBank accession No.) NM_001273(chromodomain helicase DNA binding protein 4), 유전자등록번호(GeneBank accession No.) NM_001286(chloride channel 6), 유전자등록번호(GeneBank accession No.) NM_001348(death-associated protein kinase 3), 유전자등록번호(GeneBank accession No.) NM_001386(dihydropyrimidinase-like 2), 유전자등록번호(GeneBank accession No.) NM_001388(developmentally regulated GTP binding protein 2), 유전자등록번호(GeneBank accession No.) NM_001398(enoyl Coenzyme A hydratase 1, peroxisomal), 유전자등록번호(GeneBank accession No.) NM_001440[exostoses(multiple)-like 3], 유전자등록번호(GeneBank accession No.) NM_001455(forkhead box O3), 유전자등록번호(GeneBank accession No.) NM_001521(general transcription factor IIIC, polypeptide 2, beta 110kDa), 유전자등록번호(GeneBank accession No.) NM_001524[hypocretin(orexin) neuropeptide precursor], 유전자등록번호(GeneBank accession No.) NM_001543[N-deacetylase/N-sulfotransferase(heparan glucosaminyl) 1], 유전자등록번호(GeneBank accession No.) NM_001614(actin, gamma 1), 유전자등록번호(GeneBank accession No.) NM_001620(AHNAK nucleoprotein), 유전자등록번호(GeneBank accession No.) NM_001636[solute carrier family 25(mitochondrial carrier; adenine nucleotide translocator), member 6], 유전자등록번호(GeneBank accession No.) NM_001654(v-raf murine sarcoma 3611 viral oncogene homolog), 유전자등록번호(GeneBank accession No.) NM_001659(ADP-ribosylation factor 3), 유전자등록번호(GeneBank accession No.) NM_001661(ADP-ribosylation factor-like 4D), 유전자등록번호(GeneBank accession No.) NM_001662(ADP-ribosylation factor 5), 유전자등록번호(GeneBank accession No.) NM_001684(ATPase, Ca++ transporting, plasma membrane 4), 유전자등록번호(GeneBank accession No.) NM_001728[basigin(Ok blood group)], 유전자등록번호(GeneBank accession No.) NM_001789[cell division cycle 25 homolog A(S. pombe)], 유전자등록번호(GeneBank accession No.) NM_001823(creatine kinase, brain), 유전자등록번호(GeneBank accession No.) NM_001887(crystallin, beta B1), 유전자등록번호(GeneBank accession No.) NM_001964(early growth response 1), 유전자등록번호(GeneBank accession No.) NM_001978[erythrocyte membrane protein band 4.9(dematin)], 유전자등록번호(GeneBank accession No.) NM_001986[ets variant gene 4(E1A enhancer binding protein, E1AF)], 유전자등록번호(GeneBank accession No.) NM_001997[Finkel-Biskis-Reilly murine sarcoma virus(FBR-MuSV) ubiquitously expressed(fox derived); ribosomal protein S30], 유전자등록번호(GeneBank accession No.) NM_002005(feline sarcoma oncogene), 유전자등록번호(GeneBank accession No.) NM_002046(glyceraldehyde-3-phosphate dehydrogenase), 유전자등록번호(GeneBank accession No.) NM_002060(gap junction protein, alpha 4, 37kDa), 유전자등록번호(GeneBank accession No.) NM_002086(growth factor receptor-bound protein 2), 유전자등록번호(GeneBank accession No.) NM_002119(major histocompatibility complex, class II, DO alpha), 유전자등록번호(GeneBank accession No.) NM_002307[lectin, galactoside-binding, soluble, 7(galectin 7)], 유전자등록번호(GeneBank accession No.) NM_002317(lysyl oxidase), 유전자등록번호(GeneBank accession No.) NM_002332[low density lipoprotein-related protein 1(alpha-2-macroglobulin receptor)], 유전자등록번호(GeneBank accession No.) NM_002333(low density lipoprotein receptor-related protein 3), 유전자등록번호(GeneBank accession No.) NM_002359[v-maf musculoaponeurotic fibrosarcoma oncogene homolog G(avian)], 유전자등록번호(GeneBank accession No.) NM_002415[macrophage migration inhibitory factor(glycosylation-inhibiting factor)], 유전자등록번호(GeneBank accession No.) NM_002457(mucin 2, oligomeric mucus/gel-forming), 유전자등록번호(GeneBank accession No.) NM_002466[v-myb myeloblastosis viral oncogene homolog(avian)-like 2], 유전자등록번호(GeneBank accession No.) NM_002468[myeloid differentiation primary response gene(88)], 유전자등록번호(GeneBank accession No.) NM_002542(8-oxoguanine DNA glycosylase), 유전자등록번호(GeneBank accession No.) NM_002573(platelet-activating factor acetylhydrolase, isoform Ib, gamma subunit 29kDa), 유전자등록번호(GeneBank accession No.) NM_002591[phosphoenolpyruvate carboxykinase 1(soluble)], 유전자등록번호(GeneBank accession No.) NM_002650(phosphatidylinositol 4-kinase, catalytic, alpha polypeptide), 유전자등록번호(GeneBank accession No.) NM_002660(phospholipase C, gamma 1), 유전자등록번호(GeneBank accession No.) NM_002720[protein phosphatase 4(formerly X), catalytic subunit], 유전자등록번호(GeneBank accession No.) NM_002804[proteasome(prosome, macropain) 26S subunit, ATPase, 3], 유전자등록번호(GeneBank accession No.) NM_002821(PTK7 protein tyrosine kinase 7), 유전자등록번호(GeneBank accession No.) NM_002840(protein tyrosine phosphatase, receptor type, F), 유전자등록번호(GeneBank accession No.) NM_002862(phosphorylase, glycogen; brain), 유전자등록번호(GeneBank accession No.) NM_002883(Ran GTPase activating protein 1), 유전자등록번호(GeneBank accession No.) NM_002996[chemokine(C-X3-C motif) ligand 1], 유전자등록번호(GeneBank accession No.) NM_003003[SEC14-like 1(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_003029[SHC(Src homology 2 domain containing) transforming protein 1], 유전자등록번호(GeneBank accession No.) NM_003056{Homo sapiens solute carrier family 19(folate transporter), member 1(SLC19A1), transcript variant 1, mRNA [NM_003056]}, 유전자등록번호(GeneBank accession No.) NM_003061[slit homolog 1(Drosophila)], 유전자등록번호(GeneBank accession No.) NM_003062[slit homolog 3(Drosophila)], 유전자등록번호(GeneBank accession No.) NM_003123[sialophorin(leukosialin, CD43)], 유전자등록번호(GeneBank accession No.) NM_003170[suppressor of Ty 6 homolog(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_003204[nuclear factor(erythroid-derived 2)-like 1], 유전자등록번호(GeneBank accession No.) NM_003213(TEA domain family member 4), 유전자등록번호(GeneBank accession No.) NM_003216(thyrotrophic embryonic factor), 유전자등록번호(GeneBank accession No.) NM_003224(ADP-ribosylation factor related protein 1), 유전자등록번호(GeneBank accession No.) NM_003226[trefoil factor 3(intestinal)], 유전자등록번호(GeneBank accession No.) NM_003258(thymidine kinase 1, soluble), 유전자등록번호(GeneBank accession No.) NM_003259(intercellular adhesion molecule 5, telencephalin), 유전자등록번호(GeneBank accession No.) NM_003277[claudin 5(transmembrane protein deleted in velocardiofacial syndrome)], 유전자등록번호(GeneBank accession No.) NM_003388(CAP-GLY domain containing linker protein 2), 유전자등록번호(GeneBank accession No.) NM_003486[solute carrier family 7(cationic amino acid transporter, y+ system), member 5], 유전자등록번호(GeneBank accession No.) NM_003501(acyl-Coenzyme A oxidase 3, pristanoyl), 유전자등록번호(GeneBank accession No.) NM_003513(histone cluster 1, H2ab), 유전자등록번호(GeneBank accession No.) NM_003633[ectodermal-neural cortex(with BTB-like domain)], 유전자등록번호(GeneBank accession No.) NM_003634[nipsnap homolog 1(C. elegans)], 유전자등록번호(GeneBank accession No.) NM_003683[ribosomal RNA processing 1 homolog(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_003695(lymphocyte antigen 6 complex, locus D), 유전자등록번호(GeneBank accession No.) NM_003748(aldehyde dehydrogenase 4 family, member A1), 유전자등록번호(GeneBank accession No.) NM_003751(eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa), 유전자등록번호(GeneBank accession No.) NM_003791(membrane-bound transcription factor peptidase, site 1), 유전자등록번호(GeneBank accession No.) NM_003801[glycosylphosphatidylinositol anchor attachment protein 1 homolog(yeast)], 유전자등록번호(GeneBank accession No.) NM_003891(protein Z, vitamin K-dependent plasma glycoprotein), 유전자등록번호(GeneBank accession No.) NM_003926(methyl-CpG binding domain protein 3), 유전자등록번호(GeneBank accession No.) NM_003984[solute carrier family 13(sodium-dependent dicarboxylate transporter), member 2], 유전자등록번호(GeneBank accession No.) NM_004082[dynactin 1(p150, glued homolog, Drosophila)], 유전자등록번호(GeneBank accession No.) NM_004090[dual specificity phosphatase 3(vaccinia virus phosphatase VH1-related)], 유전자등록번호(GeneBank accession No.) NM_004168[succinate dehydrogenase complex, subunit A, flavoprotein(Fp)], 유전자등록번호(GeneBank accession No.) NM_004169[serine hydroxymethyltransferase 1(soluble)], 유전자등록번호(GeneBank accession No.) NM_004214[fibroblast growth factor(acidic) intracellular binding protein], 유전자등록번호(GeneBank accession No.) NM_004286(GTP binding protein 1), 유전자등록번호(GeneBank accession No.) NM_004309[Rho GDP dissociation inhibitor(GDI) alpha], 유전자등록번호(GeneBank accession No.) NM_004341(carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase), 유전자등록번호(GeneBank accession No.) NM_004420(dual specificity phosphatase 8), 유전자등록번호(GeneBank accession No.) NM_004436(endosulfine alpha), 유전자등록번 호(GeneBank accession No.) NM_004475(flotillin 2), 유전자등록번호(GeneBank accession No.) NM_004491(glucocorticoid receptor DNA binding factor 1), 유전자등록번호(GeneBank accession No.) NM_004494[hepatoma-derived growth factor(high-mobility group protein 1-like)], 유전자등록번호(GeneBank accession No.) NM_004563[phosphoenolpyruvate carboxykinase 2(mitochondrial)], 유전자등록번호(GeneBank accession No.) NM_004566(6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3), 유전자등록번호(GeneBank accession No.) NM_004567(6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4), 유전자등록번호(GeneBank accession No.) NM_004604(syntaxin 4), 유전자등록번호(GeneBank accession No.) NM_004609[transcription factor 15(basic helix-loop-helix)], 유전자등록번호(GeneBank accession No.) NM_004639(HLA-B associated transcript 3), 유전자등록번호(GeneBank accession No.) NM_004651(ubiquitin specific peptidase 11), 유전자등록번호(GeneBank accession No.) NM_004656[BRCA1 associated protein-1(ubiquitin carboxy-terminal hydrolase)], 유전자등록번호(GeneBank accession No.) NM_004785[solute carrier family 9(sodium/hydrogen exchanger), member 3 regulator 2], 유전자등록번호(GeneBank accession No.) NM_004822(netrin 1), 유전자등록번호(GeneBank accession No.) NM_005018(programmed cell death 1), 유전자등록번호(GeneBank accession No.) NM_005077[transducin-like enhancer of split 1(E(sp1) homolog, Drosophila)], 유전자등록번호(GeneBank accession No.) NM_005082(tripartite motif-containing 25), 유전자등록번호(GeneBank accession No.) NM_005104(bromodomain containing 2), 유전자등록번호(GeneBank accession No.) NM_005119(thyroid hormone receptor associated protein 3), 유전자등록번호(GeneBank accession No.) NM_005163(v-akt murine thymoma viral oncogene homolog 1), 유전자등록번호(GeneBank accession No.) NM_005184[calmodulin 3(phosphorylase kinase, delta)], 유전자등록번호(GeneBank accession No.) NM_005216(dolichyl-diphosphooligosaccharide-protein glycosyltransferase), 유전자등록번호(GeneBank accession No.) NM_005258(GTP cyclohydrolase I feedback regulator), 유전자등록번호(GeneBank accession No.) NM_005338(huntingtin interacting protein 1), 유전자등록번호(GeneBank accession No.) NM_005412[serine hydroxymethyltransferase 2(mitochondrial)], 유전자등록번호(GeneBank accession No.) NM_005417[v-src sarcoma(Schmidt-Ruppin A-2) viral oncogene homolog(avian)], 유전자등록번호(GeneBank accession No.) NM_005468(N-acetylated alpha-linked acidic dipeptidase-like 1), 유전자등록번호(GeneBank accession No.) NM_005469(acyl-CoA thioesterase 8), 유전자등록번호(GeneBank accession No.) NM_005477(hyperpolarization activated cyclic nucleotide-gated potassium channel 4), 유전자등록번호(GeneBank accession No.) NM_005514(major histocompatibility complex, class I, B), 유전자등록번호(GeneBank accession No.) NM_005532(interferon, alpha-inducible protein 27), 유전자등록번호(GeneBank accession No.) NM_005547(involucrin), 유전자등록번호(GeneBank accession No.) NM_005572(lamin A/C), 유전자등록번호(GeneBank accession No.) NM_005581[basal cell adhesion molecule(Lutheran blood group)], 유전자등록번호(GeneBank accession No.) NM_005619(reticulon 2), 유전자등록번호(GeneBank accession No.) NM_005735[ARP1 actin-related protein 1 homolog B, centractin beta(yeast)], 유전자등록번호(GeneBank accession No.) NM_005762(tripartite motif-containing 28), 유전자등록번호(GeneBank accession No.) NM_005881(branched chain ketoacid dehydrogenase kinase), 유전자등록번호(GeneBank accession No.) NM_005934[myeloid/lymphoid or mixed-lineage leukemia(trithorax homolog, Drosophila); translocated to, 1], 유전자등록번호(GeneBank accession No.) NM_005954(metallothionein 3), 유전자등록번호(GeneBank accession No.) NM_005964(myosin, heavy chain 10, non-muscle), 유전자등록번호(GeneBank accession No.) NM_005967[NGFI-A binding protein 2(EGR1 binding protein 2)], 유전자등록번호(GeneBank accession No.) NM_005987(small proline-rich protein 1A), 유전자등록번호(GeneBank accession No.) NM_006000(tubulin, alpha 4a), 유전자등록번호(GeneBank accession No.) NM_006005[Wolfram syndrome 1(wolframin)], 유전자등록번호(GeneBank accession No.) NM_006012[ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog(E. coli)], 유전자등록번호(GeneBank accession No.) NM_006051[amyloid beta(A4) precursor protein-binding, family B, member 3], 유전자등록번호(GeneBank accession No.) NM_006058(TNFAIP3 interacting protein 1), 유전자등록번호(GeneBank accession No.) NM_006062(SMYD family member 5), 유전자등록번호(GeneBank accession No.) NM_006099(protein inhibitor of activated STAT, 3), 유전자등록번호(GeneBank accession No.) NM_006230[polymerase(DNA directed), delta 2, regulatory subunit 50kDa], 유전자등록번호(GeneBank accession No.) NM_006234[polymerase(RNA) II(DNA directed) polypeptide J, 13.3kDa], 유전자등록번호(GeneBank accession No.) NM_006266(ral guanine nucleotide dissociation stimulator), 유전자등록번호(GeneBank accession No.) NM_006295(valyl-tRNA synthetase), 유전자등록번호(GeneBank accession No.) NM_006302(glucosidase I), 유전자등록번호(GeneBank accession No.) NM_006322(tubulin, gamma complex associated protein 3), 유전자등록번호(GeneBank accession No.) NM_006328(RNA binding motif protein 14), 유전자등록번호(GeneBank accession No.) NM_006337(microspherule protein 1), 유전자등록번호(GeneBank accession No.) NM_006341[MAD2 mitotic arrest deficient-like 2(yeast)], 유전자등록번호(GeneBank accession No.) NM_006427(SIVA1, apoptosis-inducing factor), 유전자등록번호(GeneBank accession No.) NM_006429[chaperonin containing TCP1, subunit 7(eta)], 유전자등록번호(GeneBank accession No.) NM_006454(MAX dimerization protein 4), 유전자등록번호(GeneBank accession No.) NM_006464(trans-golgi network protein 2), 유전자등록번호(GeneBank accession No.) NM_006516[solute carrier family 2(facilitated glucose transporter), member 1], 유전자등록번호(GeneBank accession No.) NM_006546(insulin-like growth factor 2 mRNA binding protein 1), 유전자등록번호(GeneBank accession No.) NM_006556(phosphomevalonate kinase), 유전자등록번호(GeneBank accession No.) NM_006565[CCCTC-binding factor(zinc finger protein)], 유전자등록번호(GeneBank accession No.) NM_006623(phosphoglycerate dehydrogenase), 유전자등록번호(GeneBank accession No.) NM_006653(fibroblast growth factor receptor substrate 3), 유전자등록번호(GeneBank accession No.) NM_006663[protein phosphatase 1, regulatory(inhibitor) subunit 13 like], 유전자등록번호(GeneBank accession No.) NM_006753(surfeit 6), 유전자등록번호(GeneBank accession No.) NM_006755(transaldolase 1), 유전자등록번호(GeneBank accession No.) NM_006762(lysosomal associated multispanning membrane protein 5), 유전자등록번호(GeneBank accession No.) NM_006801[KDEL(Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1], 유전자등록번호(GeneBank accession No.) NM_006814[proteasome(prosome, macropain) inhibitor subunit 1(PI31)], 유전자등록번호(GeneBank accession No.) NM_006842(splicing factor 3b, subunit 2, 145kDa), 유전자등록번호(GeneBank accession No.) NM_006858(transmembrane emp24 protein transport domain containing 1), 유전자등록번호(GeneBank accession No.) NM_006929[superkiller viralicidic activity 2-like(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_006931[solute carrier family 2(facilitated glucose transporter), member 3], 유전자등록번호(GeneBank accession No.) NM_007024(transmembrane protein 115), 유전자등록번호(GeneBank accession No.) NM_007059[kaptin(actin binding protein)], 유전자등록번호(GeneBank accession No.) NM_007286(synaptopodin), 유전자등록번호(GeneBank accession No.) NM_007317(kinesin family member 22), 유전자등록번호(GeneBank accession No.) NM_007346(opioid growth factor receptor), 유전자등록번호(GeneBank accession No.) NM_007353[guanine nucleotide binding protein(G protein) alpha 12], 유전자등록번호(GeneBank accession No.) NM_012162(F-box and leucine-rich repeat protein 6), 유전자등록번호(GeneBank accession No.) NM_012218(interleukin enhancer binding factor 3, 90kDa), 유전자등록번호(GeneBank accession No.) NM_012227[GTP binding protein 6(putative)], 유전자등록번호(GeneBank accession No.) NM_012334(myosin X), 유전자등록번호(GeneBank accession No.) NM_012398(phosphatidylinositol-4-phosphate 5-kinase, type I, gamma), 유전자등록번호(GeneBank accession No.) NM_012404[acidic(leucine-rich) nuclear phosphoprotein 32 family, member D], 유전자등록번호(GeneBank accession No.) NM_012426(splicing factor 3b, subunit 3, 130kDa), 유전자등록번호(GeneBank accession No.) NM_012458[translocase of inner mitochondrial membrane 13 homolog(yeast)], 유전자등록번호(GeneBank accession No.) NM_012477(WW domain binding protein 1), 유전자등록번호(GeneBank accession No.) NM_012478(WW domain binding protein 2), 유전자등록번호(GeneBank accession No.) NM_013282(ubiquitin-like, containing PHD and RING finger domains, 1), 유전자등록번호(GeneBank accession No.) NM_013328(pyrroline-5- carboxylate reductase family, member 2), 유전자등록번호(GeneBank accession No.) NM_013333(epsin 1), 유전자등록번호(GeneBank accession No.) NM_013356[solute carrier family 16, member 8(monocarboxylic acid transporter 3)], 유전자등록번호(GeneBank accession No.) NM_013379(dipeptidyl-peptidase 7), 유전자등록번호(GeneBank accession No.) NM_013389[NPC1(Niemann-Pick disease, type C1, gene)-like 1], 유전자등록번호(GeneBank accession No.) NM_013392(nuclear receptor binding protein 1), 유전자등록번호(GeneBank accession No.) NM_013407(deoxyhypusine synthase), 유전자등록번호(GeneBank accession No.) NM_013430(gamma-glutamyltransferase 1), 유전자등록번호(GeneBank accession No.) NM_013442[stomatin(EPB72)-like 2], 유전자등록번호(GeneBank accession No.) NM_013976(glutaryl-Coenzyme A dehydrogenase), 유전자등록번호(GeneBank accession No.) NM_014007(zinc finger and BTB domain containing 43), 유전자등록번호(GeneBank accession No.) NM_014064(chromosome 9 open reading frame 32), 유전자등록번호(GeneBank accession No.) NM_014225[protein phosphatase 2(formerly 2A), regulatory subunit A , alpha isoform], 유전자등록번호(GeneBank accession No.) NM_014281(fuse-binding protein-interacting repressor), 유전자등록번호(GeneBank accession No.) NM_014316(calcium regulated heat stable protein 1, 24kDa), 유전자등록번호(GeneBank accession No.) NM_014328(RUN and SH3 domain containing 1), 유전자등록번호(GeneBank accession No.) NM_014341[mitochondrial carrier homolog 1(C. elegans)], 유전자등록번호(GeneBank accession No.) NM_014392[DNA segment on chromosome 4(unique) 234 expressed sequence], 유전자등록번호(GeneBank accession No.) NM_014437[solute carrier family 39(zinc transporter), member 1], 유전자등록번호(GeneBank accession No.) NM_014551(non-SMC condensin II complex, subunit H2), 유전자등록번호(GeneBank accession No.) NM_014587[SRY(sex determining region Y)-box 8], 유전자등록번호(GeneBank accession No.) NM_014675(ciliary rootlet coiled-coil, rootletin), 유전자등록번호(GeneBank accession No.) NM_014678(SAPS domain family, member 2), 유전자등록번호(GeneBank accession No.) NM_014706(squamous cell carcinoma antigen recognized by T cells 3), 유전자등록번호(GeneBank accession No.) NM_014719(KIAA0738 gene product), 유전자등록번호(GeneBank accession No.) NM_014727(myeloid/lymphoid or mixed-lineage leukemia 4), 유전자등록번호(GeneBank accession No.) NM_014738(KIAA0195), 유전자등록번호(GeneBank accession No.) NM_014748(sorting nexin 17), 유전자등록번호(GeneBank accession No.) NM_014762(24-dehydrocholesterol reductase), 유전자등록번호(GeneBank accession No.) NM_014780(cullin 7), 유전자등록번호(GeneBank accession No.) NM_014952(bromo adjacent homology domain containing 1), 유전자등록번호(GeneBank accession No.) NM_015024(exportin 7), 유전자등록번호(GeneBank accession No.) NM_015075(IQ motif and Sec7 domain 2), 유전자등록번호(GeneBank accession No.) NM_015111(Nedd4 binding protein 3), 유전자등록번호(GeneBank accession No.) NM_015171(exportin 6), 유전자등록번호(GeneBank accession No.) NM_015198[cordon-bleu homolog(mouse)], 유전자등록번호(GeneBank accession No.) NM_015289[vacuolar protein sorting 39 homolog(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_015319[tensin like C1 domain containing phosphatase(tensin 2)], 유전자등록번호(GeneBank accession No.) NM_015353(potassium channel tetramerisation domain containing 2), 유전자등록번호(GeneBank accession No.) NM_015354(nucleoporin 188kDa), 유전자등록번호(GeneBank accession No.) NM_015466(protein tyrosine phosphatase, non-receptor type 23), 유전자등록번호(GeneBank accession No.) NM_015517(MBD2-interacting zinc finger), 유전자등록번호(GeneBank accession No.) NM_015528(ring finger protein 167), 유전자등록번호(GeneBank accession No.) NM_015533[dihydroxyacetone kinase 2 homolog(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_015603(coiled-coil domain containing 9), 유전자등록번호(GeneBank accession No.) NM_015853(SAPK substrate protein 1), 유전자등록번호(GeneBank accession No.) NM_015914(thioredoxin domain containing 11), 유전자등록번호(GeneBank accession No.) NM_015926(testis expressed 264), 유전자등록번호(GeneBank accession No.) NM_015950(mitochondrial ribosomal protein L2), 유전자등록번호(GeneBank accession No.) NM_015953(nitric oxide synthase interacting protein), 유전자등록번호(GeneBank accession No.) NM_015965[NADH dehydrogenase(ubiquinone) 1 alpha subcomplex, 13], 유전자등록번호(GeneBank accession No.) NM_016022[anterior pharynx defective 1 homolog A(C. elegans)], 유전자등록번호(GeneBank accession No.) NM_016332(selenoprotein X, 1), 유전자등록번호(GeneBank accession No.) NM_016437(tubulin, gamma 2), 유전자등록번호(GeneBank accession No.) NM_016438(HIG1 domain family, member 1B), 유전자등록번호(GeneBank accession No.) NM_016492(RAN guanine nucleotide release factor), 유전자등록번호(GeneBank accession No.) NM_016564(cell cycle exit and neuronal differentiation 1), 유전자등록번호(GeneBank accession No.) NM_016733(LIM domain kinase 2), 유전자등록번호(GeneBank accession No.) NM_017422(calmodulin-like 5), 유전자등록번호(GeneBank accession No.) NM_017491(WD repeat domain 1), 유전자등록번호(GeneBank accession No.) NM_017510(transmembrane emp24 protein transport domain containing 9), 유전자등록번호(GeneBank accession No.) NM_017626(DnaJ(Hsp40) homolog, subfamily B, member 12), 유전자등록번호(GeneBank accession No.) NM_017668[nudE nuclear distribution gene E homolog 1(A. nidulans)], 유전자등록번호(GeneBank accession No.) NM_017702(hypothetical protein FLJ20186), 유전자등록번호(GeneBank accession No.) NM_017727(hypothetical protein FLJ20254), 유전자등록번호(GeneBank accession No.) NM_017750[retinol saturase(all-trans-retinol 13,14-reductase)], 유전자등록번호(GeneBank accession No.) NM_017784(oxysterol binding protein-like 10), 유전자등록번호(GeneBank accession No.) NM_017803[dihydrouridine synthase 2-like, SMM1 homolog(S. cerevisiae)], 유전 자등록번호(GeneBank accession No.) NM_017854(transmembrane protein 160), 유전자등록번호(GeneBank accession No.) NM_017966[vacuolar protein sorting 37 homolog C(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_018035(hypothetical protein FLJ10241), 유전자등록번호(GeneBank accession No.) NM_018067(MAP7 domain containing 1), 유전자등록번호(GeneBank accession No.) NM_018081(WD repeat domain 79), 유전자등록번호(GeneBank accession No.) NM_018110(docking protein 4), 유전자등록번호(GeneBank accession No.) NM_018111(hypothetical protein FLJ10490), 유전자등록번호(GeneBank accession No.) NM_018125[Rho guanine nucleotide exchange factor(GEF) 10-like], 유전자등록번호(GeneBank accession No.) NM_018150(chromosome 1 open reading frame 164), 유전자등록번호(GeneBank accession No.) NM_018174(microtubule-associated protein 1S), 유전자등록번호(GeneBank accession No.) NM_018226[arginyl aminopeptidase(aminopeptidase B)-like 1], 유전자등록번호(GeneBank accession No.) NM_018231(amino acid transporter), 유전자등록번호(GeneBank accession No.) NM_018358[ATP-binding cassette, sub-family F(GCN20), member 3], 유전자등록번호(GeneBank accession No.) NM_018419[SRY(sex determining region Y)-box 18], 유전자등록번호(GeneBank accession No.) NM_018490(leucine-rich repeat-containing G protein-coupled receptor 4), 유전자등록번호(GeneBank accession No.) NM_018645[hairy and enhancer of split 6(Drosophila)], 유전자등록번호(GeneBank accession No.) NM_018660(zinc finger protein 395), 유전자등록번 호(GeneBank accession No.) NM_018670[mesoderm posterior 1 homolog(mouse)], 유전자등록번호(GeneBank accession No.) NM_018687(hepatocellular carcinoma-associated gene TD26), 유전자등록번호(GeneBank accession No.) NM_018964[solute carrier family 37(glycerol-3-phosphate transporter), member 1], 유전자등록번호(GeneBank accession No.) NM_018973(dolichyl-phosphate mannosyltransferase polypeptide 3), 유전자등록번호(GeneBank accession No.) NM_018979(WNK lysine deficient protein kinase 1), 유전자등록번호(GeneBank accession No.) NM_020158(exosome component 5), 유전자등록번호(GeneBank accession No.) NM_020172(signal peptide peptidase-like 2B), 유전자등록번호(GeneBank accession No.) NM_020210[sema domain, immunoglobulin domain(Ig), transmembrane domain(TM) and short cytoplasmic domain,(semaphorin) 4B], 유전자등록번호(GeneBank accession No.) NM_020246[solute carrier family 12(potassium/chloride transporters), member 9], 유전자등록번호(GeneBank accession No.) NM_020834(homeobox and leucine zipper encoding), 유전자등록번호(GeneBank accession No.) NM_020954(KIAA1618), 유전자등록번호(GeneBank accession No.) NM_021095[solute carrier family 5(sodium-dependent vitamin transporter), member 6], 유전자등록번호(GeneBank accession No.) NM_021170[hairy and enhancer of split 4(Drosophila)], 유전자등록번호(GeneBank accession No.) NM_021247(protamine 3), 유전자등록번호(GeneBank accession No.) NM_021574(breakpoint cluster region), 유전자등록번호(GeneBank accession No.) NM_021603(FXYD domain containing ion transport regulator 2), 유전자등록번호(GeneBank accession No.) NM_021807(exocyst complex component 4), 유전자등록번호(GeneBank accession No.) NM_021932[resistance to inhibitors of cholinesterase 8 homolog A(C. elegans)], 유전자등록번호(GeneBank accession No.) NM_021939(FK506 binding protein 10, 65 kDa), 유전자등록번호(GeneBank accession No.) NM_021969(nuclear receptor subfamily 0, group B, member 2), 유전자등록번호(GeneBank accession No.) NM_022171(T-cell leukemia translocation altered gene), 유전자등록번호(GeneBank accession No.) NM_022372(G protein beta subunit-like), 유전자등록번호(GeneBank accession No.) NM_022449(RAB17, member RAS oncogene family), 유전자등록번호(GeneBank accession No.) NM_022460(HCLS1 binding protein 3), 유전자등록번호(GeneBank accession No.) NM_022833(family with sequence similarity 129, member B), 유전자등록번호(GeneBank accession No.) NM_022908(5'-nucleotidase domain containing 2), 유전자등록번호(GeneBank accession No.) NM_022911(solute carrier family 26, member 6), 유전자등록번호(GeneBank accession No.) NM_022914[adrenocortical dysplasia homolog(mouse)], 유전자등록번호(GeneBank accession No.) NM_023018(NAD kinase), 유전자등록번호(GeneBank accession No.) NM_023939(hypothetical protein MGC2752), 유전자등록번호(GeneBank accession No.) NM_023947(chitinase domain containing 1), 유전자등록번호(GeneBank accession No.) NM_024012[5-hydroxytryptamine(serotonin) receptor 5A], 유전자 등록번호(GeneBank accession No.) NM_024066(prion protein interacting protein), 유전자등록번호(GeneBank accession No.) NM_024103[solute carrier family 25(mitochondrial carrier; phosphate carrier), member 23], 유전자등록번호(GeneBank accession No.) NM_024109(chromosome 16 open reading frame 68), 유전자등록번호(GeneBank accession No.) NM_024294(chromosome 6 open reading frame 106), 유전자등록번호(GeneBank accession No.) NM_024296(coiled-coil domain containing 28B), 유전자등록번호(GeneBank accession No.) NM_024310[pleckstrin homology domain containing, family F(with FYVE domain) member 1], 유전자등록번호(GeneBank accession No.) NM_024516(chromosome 16 open reading frame 53), 유전자등록번호(GeneBank accession No.) NM_024536(chondroitin polymerizing factor), 유전자등록번호(GeneBank accession No.) NM_024671(zinc finger protein 768), 유전자등록번호(GeneBank accession No.) NM_024710(isochorismatase domain containing 2), 유전자등록번호(GeneBank accession No.) NM_024827(histone deacetylase 11), 유전자등록번호(GeneBank accession No.) NM_024859(MAGI family member, X-linked), 유전자등록번호(GeneBank accession No.) NM_024872(docking protein 3), 유전자등록번호(GeneBank accession No.) NM_024955(FAD-dependent oxidoreductase domain containing 2), 유전자등록번호(GeneBank accession No.) NM_025021(CREB regulated transcription coactivator 1), 유전자등록번호(GeneBank accession No.) NM_025083[enhancer of mRNA decapping 3 homolog(S. cerevisiae)], 유전자등 록번호(GeneBank accession No.) NM_025084(hypothetical protein FLJ22795), 유전자등록번호(GeneBank accession No.) NM_025139(armadillo repeat containing 9), 유전자등록번호(GeneBank accession No.) NM_025207[FAD1 flavin adenine dinucleotide synthetase homolog(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_025227(bactericidal/permeability-increasing protein-like 1), 유전자등록번호(GeneBank accession No.) NM_025233(Coenzyme A synthase), 유전자등록번호(GeneBank accession No.) NM_030578(hypothetical protein MGC4093), 유전자등록번호(GeneBank accession No.) NM_030891(leucine rich repeat containing 3), 유전자등록번호(GeneBank accession No.) NM_030900(transforming growth factor beta regulator 4), 유전자등록번호(GeneBank accession No.) NM_030966(keratin associated protein 1-3), 유전자등록번호(GeneBank accession No.) NM_031209[queuine tRNA-ribosyltransferase 1(tRNA-guanine transglycosylase)], 유전자등록번호(GeneBank accession No.) NM_031232(amyloid beta(A4) precursor protein-binding, family A, member 2 binding protein), 유전자등록번호(GeneBank accession No.) NM_031918(Kruppel-like factor 16), 유전자등록번호(GeneBank accession No.) NM_032125(chromosome 1 open reading frame 160), 유전자등록번호(GeneBank accession No.) NM_032179{Homo sapiens cleavage and polyadenylation specific factor 3-like(CPSF3L), transcript variant 2, mRNA [NM_032179]}, 유전자등록번호(GeneBank accession No.) NM_032271(TNF receptor-associated factor 7), 유전자등록번호(GeneBank accession No.) NM_032283(zinc finger, DHHC-type containing 18), 유전자등록번호(GeneBank accession No.) NM_032326(transmembrane protein 175), 유전자등록번호(GeneBank accession No.) NM_032334(chromosome 8 open reading frame 53), 유전자등록번호(GeneBank accession No.) NM_032349[nudix(nucleoside diphosphate linked moiety X)-type motif 16-like 1], 유전자등록번호(GeneBank accession No.) NM_032389(zinc finger protein 289, ID1 regulated), 유전자등록번호(GeneBank accession No.) NM_032569(cytokine-like nuclear factor n-pac), 유전자등록번호(GeneBank accession No.) NM_032689(zinc finger protein 607), 유전자등록번호(GeneBank accession No.) NM_032789[poly(ADP-ribose) polymerase family, member 10], 유전자등록번호(GeneBank accession No.) NM_032918(RAS-like, estrogen-regulated, growth inhibitor), 유전자등록번호(GeneBank accession No.) NM_032928(transmembrane protein 141), 유전자등록번호(GeneBank accession No.) NM_033036(galactose-3-O-sulfotransferase 3), 유전자등록번호(GeneBank accession No.) NM_033046(rhotekin), 유전자등록번호(GeneBank accession No.) NM_033060(keratin associated protein 4-10), 유전자등록번호(GeneBank accession No.) NM_033178(double homeobox, 4), 유전자등록번호(GeneBank accession No.) NM_033184(keratin associated protein 2-4), 유전자등록번호(GeneBank accession No.) NM_033260(forkhead box Q1), 유전자등록번호(GeneBank accession No.) NM_033310(potassium channel, subfamily K, member 4), 유전자등록번호(GeneBank accession No.) NM_033388[ATG16 autophagy related 16-like 2(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_033453[inosine triphosphatase(nucleoside triphosphate pyrophosphatase)], 유전자등록번호(GeneBank accession No.) NM_033502(transcriptional regulating factor 1), 유전자등록번호(GeneBank accession No.) NM_052853(aarF domain containing kinase 2), 유전자등록번호(GeneBank accession No.) NM_052865(chromosome 20 open reading frame 72), 유전자등록번호(GeneBank accession No.) NM_052880(phosphoinositide-3-kinase interacting protein 1), 유전자등록번호(GeneBank accession No.) NM_052948(sorting nexin 26), 유전자등록번호(GeneBank accession No.) NM_057161(kelch domain containing 3), 유전자등록번호(GeneBank accession No.) NM_058219(exosome component 6), 유전자등록번호(GeneBank accession No.) NM_080686(HLA-B associated transcript 2), 유전자등록번호(GeneBank accession No.) NM_080792(signal-regulatory protein alpha), 유전자등록번호(GeneBank accession No.) NM_080825(chromosome 20 open reading frame 144), 유전자등록번호(GeneBank accession No.) NM_080863(ankyrin repeat and SOCS box-containing 16), 유전자등록번호(GeneBank accession No.) NM_130468[carbohydrate(N-acetylgalactosamine 4-0) sulfotransferase 14], 유전자등록번호(GeneBank accession No.) NM_133177(protein tyrosine phosphatase, receptor type, U), 유전자등록번호(GeneBank accession No.) NM_133371(myozenin 3), 유전자등록번호(GeneBank accession No.) NM_138322(proprotein convertase subtilisin/kexin type 6), 유전자등록번호(GeneBank accession No.) NM_138325(proprotein convertase subtilisin/kexin type 6), 유전자등록번호(GeneBank accession No.) NM_138361(leucine rich repeat and sterile alpha motif containing 1), 유전자등록번호(GeneBank accession No.) NM_138385(transmembrane protein 129), 유전자등록번호(GeneBank accession No.) NM_138387(glucose 6 phosphatase, catalytic, 3), 유전자등록번호(GeneBank accession No.) NM_138412[retinol dehydrogenase 13(all-trans/9-cis)], 유전자등록번호(GeneBank accession No.) NM_138578(BCL2-like 1), 유전자등록번호(GeneBank accession No.) NM_138763(BCL2-associated X protein), 유전자등록번호(GeneBank accession No.) NM_139067(SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2), 유전자등록번호(GeneBank accession No.) NM_139280[ORM1-like 3(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_145040(protein kinase C, delta binding protein), 유전자등록번호(GeneBank accession No.) NM_145110{Homo sapiens mitogen-activated protein kinase kinase 3(MAP2K3), transcript variant C, mRNA [NM_145110]}, 유전자등록번호(GeneBank accession No.) NM_145179{Homo sapiens chromosome 21 open reading frame 93(C21orf93), mRNA [NM_145179]}, 유전자등록번호(GeneBank accession No.) NM_145343(apolipoprotein L, 1), 유전자등록번호(GeneBank accession No.) NM_145345(UBX domain containing 5), 유전자등록번호(GeneBank accession No.) NM_145685[BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB(S. cerevisiae)], 유전자등록번 호(GeneBank accession No.) NM_145870[glutathione transferase zeta 1(maleylacetoacetate isomerase)], 유전자등록번호(GeneBank accession No.) NM_145904(high mobility group AT-hook 1), 유전자등록번호(GeneBank accession No.) NM_147780(cathepsin B), 유전자등록번호(GeneBank accession No.) NM_152243[CDC42 effector protein(Rho GTPase binding) 1], 유전자등록번호(GeneBank accession No.) NM_152272(CHMP family, member 7), 유전자등록번호(GeneBank accession No.) NM_152299(non-SMC condensin II complex, subunit H2), 유전자등록번호(GeneBank accession No.) NM_152326(ankyrin repeat domain 9), 유전자등록번호(GeneBank accession No.) NM_152600(zinc finger protein 579), 유전자등록번호(GeneBank accession No.) NM_153002(G protein-coupled receptor 156), 유전자등록번호(GeneBank accession No.) NM_153200(endothelial differentiation-related factor 1), 유전자등록번호(GeneBank accession No.) NM_153236(GTPase, IMAP family member 7), 유전자등록번호(GeneBank accession No.) NM_153253(signal-induced proliferation-associated gene 1), 유전자등록번호(GeneBank accession No.) NM_153284(hyaluronoglucosaminidase 1), 유전자등록번호(GeneBank accession No.) NM_153320[solute carrier family 22(organic anion transporter), member 7], 유전자등록번호(GeneBank accession No.) NM_153461(interleukin 17 receptor C), 유전자등록번호(GeneBank accession No.) NM_153631(homeobox A3), 유전자등록번호(GeneBank accession No.) NM_153824(pyrroline-5-carboxylate reductase 1), 유전자등록번호(GeneBank accession No.) NM_153827[misshapen-like kinase 1(zebrafish)], 유전자등록번호(GeneBank accession No.) NM_170692(RAS protein activator like 2), 유전자등록번호(GeneBank accession No.) NM_170726(aldehyde dehydrogenase 4 family, member A1), 유전자등록번호(GeneBank accession No.) NM_171982(tripartite motif-containing 35), 유전자등록번호(GeneBank accession No.) NM_172027[ankyrin repeat and BTB(POZ) domain containing 1], 유전자등록번호(GeneBank accession No.) NM_172171[calcium/calmodulin-dependent protein kinase(CaM kinase) II gamma], 유전자등록번호(GeneBank accession No.) NM_173179(solute carrier family 35, member C2), 유전자등록번호(GeneBank accession No.) NM_173354(SNF1-like kinase), 유전자등록번호(GeneBank accession No.) NM_173467[malonyl CoA:ACP acyltransferase(mitochondrial)], 유전자등록번호(GeneBank accession No.) NM_173546(kelch domain containing 8B), 유전자등록번호(GeneBank accession No.) NM_173596[solute carrier family 39(metal ion transporter), member 5], 유전자등록번호(GeneBank accession No.) NM_173687(chromosome 8 open reading frame 31), 유전자등록번호(GeneBank accession No.) NM_173728[Rho guanine nucleotide exchange factor(GEF) 15], 유전자등록번호(GeneBank accession No.) NM_174903(ring finger protein 151), 유전자등록번호(GeneBank accession No.) NM_174910(t-complex-associated-testis-expressed 3), 유전자등록번호(GeneBank accession No.) NM_174913(chromosome 14 open reading frame 21), 유전자등록번호(GeneBank accession No.) NM_174937(transcription elongation regulator 1-like), 유전자등록번호(GeneBank accession No.) NM_174983(chromosome 19 open reading frame 28), 유전자등록번호(GeneBank accession No.) NM_175609(ADP-ribosylation factor GTPase activating protein 1), 유전자등록번호(GeneBank accession No.) NM_175709(chromobox homolog 7), 유전자등록번호(GeneBank accession No.) NM_175734(chromosome 17 open reading frame 74), 유전자등록번호(GeneBank accession No.) NM_175852(taxilin alpha), 유전자등록번호(GeneBank accession No.) NM_177967(UBA domain containing 2), 유전자등록번호(GeneBank accession No.) NM_177987(tubulin, beta 8), 유전자등록번호(GeneBank accession No.) NM_178150(F-box protein, helicase, 18), 유전자등록번호(GeneBank accession No.) NM_178176(monoacylglycerol O-acyltransferase 3), 유전자등록번호(GeneBank accession No.) NM_178326[ATG4 autophagy related 4 homolog B(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_178352(late cornified envelope 1D), 유전자등록번호(GeneBank accession No.) NM_178433(late cornified envelope 3B), 유전자등록번호(GeneBank accession No.) NM_178518(transmembrane protein 102), 유전자등록번호(GeneBank accession No.) NM_178519(chromosome 17 open reading frame 55), 유전자등록번호(GeneBank accession No.) NM_178819[1-acylglycerol-3-phosphate O-acyltransferase 6(lysophosphatidic acid acyltransferase, zeta)], 유전자등록번호(GeneBank accession No.) NM_178830(chromosome 19 open reading frame 47), 유전자등록번호(GeneBank accession No.) NM_181468(integrin beta 4 binding protein), 유전자등록번호(GeneBank accession No.) NM_181718(aspartate beta-hydroxylase domain containing 1), 유전자등록번호(GeneBank accession No.) NM_181738(peroxiredoxin 2), 유전자등록번호(GeneBank accession No.) NM_181809(bone morphogenetic protein 8a), 유전자등록번호(GeneBank accession No.) NM_182470(pyruvate kinase, muscle), 유전자등록번호(GeneBank accession No.) NM_182557(B-cell CLL/lymphoma 9-like), 유전자등록번호(GeneBank accession No.) NM_182614(family with sequence similarity 70, member B), 유전자등록번호(GeneBank accession No.) NM_182947(RAC/CDC42 exchange factor), 유전자등록번호(GeneBank accession No.) NM_182983[hepsin(transmembrane protease, serine 1)], 유전자등록번호(GeneBank accession No.) NM_183352[SEC13 homolog(S. cerevisiae)], 유전자등록번호(GeneBank accession No.) NM_184041(aldolase A, fructose-bisphosphate), 유전자등록번호(GeneBank accession No.) NM_194278(chromosome 14 open reading frame 43), 유전자등록번호(GeneBank accession No.) NM_194302(coiled-coil domain containing 108), 유전자등록번호(GeneBank accession No.) NM_198040[polyhomeotic homolog 2(Drosophila)], 유전자등록번호(GeneBank accession No.) NM_198061[carboxylesterase 2(intestine, liver)], 유전자등록번호(GeneBank accession No.) NM_198149(transmembrane protein 58), 유전자등록번호(GeneBank accession No.) NM_198181(hypothetical protein LOC440295), 유전자등록번호(GeneBank accession No.) NM_198284{Homo sapiens hypothetical protein LOC349114(LOC349114), mRNA [NM_198284]}, 유전자 등록번호(GeneBank accession No.) NM_198334(glucosidase, alpha; neutral AB), 유전자등록번호(GeneBank accession No.) NM_199037(sodium channel, voltage-gated, type I, beta), 유전자등록번호(GeneBank accession No.) NM_199345(similar to phosphatidylinositol 4-kinase alpha), 유전자등록번호(GeneBank accession No.) NM_201382(plectin 1, intermediate filament binding protein 500kDa), 유전자등록번호(GeneBank accession No.) NM_201535(NDRG family member 2), 유전자등록번호(GeneBank accession No.) NM_201629[tight junction protein 2(zona occludens 2)], 유전자등록번호(GeneBank accession No.) NM_203344(SERTA domain containing 3), 유전자등록번호(GeneBank accession No.) NM_203351(mitogen-activated protein kinase kinase kinase 3), 유전자등록번호(GeneBank accession No.) NM_203393(hypothetical gene supported by BC031661), 유전자등록번호(GeneBank accession No.) NM_206949(family with sequence similarity 14, member B), 유전자등록번호(GeneBank accession No.) NM_206962(protein arginine methyltransferase 2), 유전자등록번호(GeneBank accession No.) NM_207107(UCHL5 interacting protein), 유전자등록번호(GeneBank accession No.) NM_207370(G protein-coupled receptor 153), 유전자등록번호(GeneBank accession No.) NM_213604(ADAMTS-like 5), 유전자등록번호(GeneBank accession No.) NM_213647(fibroblast growth factor receptor 4), 유전자등록번호(GeneBank accession No.) NM_214710(protease, serine-like 1), 유전자등록번호(GeneBank accession No.) NP106190{GB|X76785.1|CAA54180.1 hypothetical protein [NP106190]}, 유전자등록번호(GeneBank accession No.) NR_002187(hypothetical protein LOC286016), 유전자등록번호(GeneBank accession No.) NR_002603(nuclear pore complex interacting protein pseudogene), 유전자등록번호(GeneBank accession No.) NR_002797(hypothetical protein LOC255783), 유전자등록번호(GeneBank accession No.) NR_002837(ubiquitin-conjugating enzyme E2M pseudogene 1), 유전자등록번호(GeneBank accession No.) NR_002929(actin pseudogene), 유전자등록번호(GeneBank accession No.) S72422[dihydrolipoamide S-succinyltransferase pseudogene(E2 component of 2-oxo-glutarate complex)], 유전자등록번호(GeneBank accession No.) U21012[Clone 120Pa immunoglobulin light chain variable region(VkJ)], 유전자등록번호(GeneBank accession No.) U35612[SRY(sex determining region Y)-box 12], 유전자등록번호(GeneBank accession No.) U63828(chromosome 20 open reading frame 181), 유전자등록번호(GeneBank accession No.) U68019(SMAD family member 3), 유전자등록번호(GeneBank accession No.) XM_028217(hypothetical LOC90024), 유전자등록번호(GeneBank accession No.) XM_293937{PREDICTED: Homo sapiens similar to ankyrin repeat domain 33(LOC345711), mRNA [XM_293937]}, 유전자등록번호(GeneBank accession No.) XM_373788{full-length cDNA clone CS0DF014YD20 of Fetal brain of Homo sapiens(human). [CR593500]}, 유전자등록번호(GeneBank accession No.) XM_926717(similar to actin, alpha 2, smooth muscle, aorta), 유전자등록번호(GeneBank accession No.) XM_927514[similar to Elongation factor 1-delta(EF-1-delta)(Antigen NY-CO-4)], 유전자등록번호(GeneBank accession No.) XM_928285{PREDICTED: Homo sapiens similar to protein expressed in prostate, ovary, testis, and placenta 15, transcript variant 1(LOC653239), mRNA [XM_928285]}, 유전자등록번호(GeneBank accession No.) XM_928640{Homo sapiens N-deacetylase/N-sulfotransferase(heparan glucosaminyl) 2, mRNA(cDNA clone IMAGE:4665687), partial cds. [BC018681]}, 유전자등록번호(GeneBank accession No.) XM_932013{PREDICTED: Homo sapiens hypothetical protein LOC644134(LOC644134), mRNA [XM_932013]}, 유전자등록번호(GeneBank accession No.) XM_936535{PREDICTED: Homo sapiens hypothetical protein LOC648245, transcript variant 1(LOC648245), mRNA [XM_936535]}, 유전자등록번호(GeneBank accession No.) XM_937698(similar to synaptogyrin 2), 유전자등록번호(GeneBank accession No.) XM_943677{Homo sapiens hypothetical LOC389634, mRNA(cDNA clone IMAGE:4157715). [BC037255]}, 유전자등록번호(GeneBank accession No.) XM_946056{PREDICTED: Homo sapiens hypothetical protein LOC643669, transcript variant 2(LOC643669), mRNA [XM_933624]}.Gene access number (GeneBank accession No. ) AA449398 (castor zinc finger 1), Gene accession number (GeneBank accession No. ) AB040942 (coiled-coil domain containing 88C), gene registration number (GeneBank accession No. ) AB065089 (ribosomal protein S2), gene registration number (GeneBank accession No. ) AF005082 (
본 발명자들은 헥사클로로벤젠에 대한 노출 여부 확인용 바이오마커를 발굴하기 위하여, 헥사클로로벤젠을 인간 간암 세포주(HepG2)에 처리하여 85% 생존 농도(IC15 dose)의 헥사클로로벤젠(도 1 참조)을 인간 간암 세포주에 처리한 후, mRNA를 분리하여 4X44 k 올리고마이크로어레이 칩 Human whole genome microarray(Agilent, USA)를 이용하여 유전자 발현양상을 분석하였다(도 2 참조). 분석시 p-값(p-value)이 0.001 이하이고, Cy5/Cy3 비율이 1.5배 이상인 경우 발현이 증가한 유전자로 분류하였고, 0.667배 이하인 경우 발현 감소한 유전자로 분류하였다. 분석 결과, 발현이 증가한 유전자는 0.21%(45,220개의 유전자 중 96개), 발현이 감소한 유전자는 1.364%(45,220개의 유전자 중 617개)임을 확인하였다. 상기 유전자들 중 기능이 알려진 유전자들을 생물학적 방법(biological process)에 따라 분류한 결과, 스트레스반응(response to stress), 번역(translation), 지혈(hemostasis), 스테로이드 대사 과정(steroid metabolic process), 면역 작용자 과정(immune effector process), 섬유소 분해(fibrinolysis), 세포 분화(cell differentiation), 세포 자살(apoptosis), 지질 대사 과정(lipid metabolic process), 전사 규칙(positive regulation of transcription), 아미노산 대사 과정(amino acid metabolic process) 또는 스테롤 대사 과정(sterol metabolic process)와 관련된 유전자로 분류되었다(표 2 및 표 3 참조). 상기 유전자들은 본 발명에서 사용한 헥사클로로벤젠을 처리했을 때, 인간 간암 세포주에서 독성과 관련이 있다는 보고는 없다.In order to identify a biomarker for confirming exposure to hexachlorobenzene, the present inventors treated hexachlorobenzene in a human liver cancer cell line (HepG2) to treat hexachlorobenzene at 85% survival concentration (IC15 dose) (see FIG. 1). After treatment with human liver cancer cell lines, mRNA was isolated and analyzed for gene expression using a 4 × 44 k oligomicroarray chip, Human whole genome microarray (Agilent, USA) (see FIG. 2). When the p-value was less than 0.001 and the Cy5 / Cy3 ratio was 1.5 times or more, the expression was classified as a gene with increased expression. As a result, 0.21% (96 out of 45,220) genes increased expression, and 1.364% (617 out of 45,220 genes) decreased expression. Among the genes, genes whose functions are known are classified according to biological methods, resulting in response to stress, translation, hemostasis, steroid metabolic process, and immune action. Immune effector process, fibrinolysis, cell differentiation, apoptosis, lipid metabolic process, positive regulation of transcription, amino acid metabolism process genes associated with acid metabolic process or sterol metabolic process (see Table 2 and Table 3). The genes have not been reported to be associated with toxicity in human liver cancer cell lines when treated with hexachlorobenzene used in the present invention.
또한, 본 발명은 상기 바이오마커 유전자 서열의 전부 또는 일부를 포함하는 올리고뉴클레오티드 또는 그의 상보적인 올리고뉴클레오티드가 집적된, 헥사클로로벤젠에 대한 노출 여부 확인용 마이크로어레이 칩을 제공한다.The present invention also provides a microarray chip for confirming exposure to hexachlorobenzene in which an oligonucleotide including all or part of the biomarker gene sequence or its complementary oligonucleotide is integrated.
상기 올리고뉴클레오티드 또는 이의 상보가닥 분자는 상기 바이오마커 유전 자의 15 내지 50개, 바람직하게는 15 내지 30개, 더욱 바람직하게는 18 내지 25개의 핵산을 포함한다.The oligonucleotide or complementary strand molecule thereof comprises 15 to 50, preferably 15 to 30, more preferably 18 to 25 nucleic acids of the biomarker gene.
본 발명의 DNA 마이크로어레이 칩은 당업자에게 알려진 방법으로 제작할 수 있다. 상기 마이크로어레이 칩을 제작하는 방법은 하기와 같다. 상기 탐색된 바이오마커를 탐침 DNA 분자로 이용하여 DNA 칩의 기판상에 고정화시키기 위해 파이조일렉트릭(piezoelectric) 방식을 이용한 마이크로파이펫팅(micropipetting)법 또는 핀(pin) 형태의 스폿터(spotter)를 이용한 방법 등을 사용하는 것이 바람직하나 이에 한정되는 것은 아니다. 상기 DNA 마이크로어레이 칩의 기판은 아미노-실란(amino-silane), 폴리-L-라이신(poly-L-lysine) 및 알데히드(aldehyde)로 이루어진 군에서 선택되는 하나의 활성기가 코팅된 것이 바람직하나 이에 한정되는 것은 아니다. 또한, 상기 기판은 슬라이드 글래스, 플라스틱, 금속, 실리콘, 나일론 막, 및 니트로셀룰로스 막(nitrocellulose membrane)으로 이루어진 군에서 선택될 수 있으나 이에 제한되는 것은 아니다. The DNA microarray chip of the present invention can be produced by methods known to those skilled in the art. A method of fabricating the microarray chip is as follows. In order to immobilize the searched biomarker as a probe DNA molecule on a substrate of a DNA chip, a micropipetting method using a piezoelectric method or a pin type spotter is used. It is preferable to use a method and the like, but is not limited thereto. The substrate of the DNA microarray chip is preferably coated with one activator selected from the group consisting of amino-silane, poly-L-lysine, and aldehyde, But is not limited thereto. In addition, the substrate may be selected from the group consisting of slide glass, plastic, metal, silicon, nylon membrane, and nitrocellulose membrane, but is not limited thereto.
또한, 본 발명은 헥사클로로벤젠에 대한 노출 여부 확인을 위한 상기 마커의 검출 방법을 제공한다.In addition, the present invention provides a method for detecting the marker for confirming the exposure to hexachlorobenzene.
구체적으로, 본 발명은Specifically, the present invention provides
1) 실험군인 피검체 유래 인간 체세포와 대조군 체세포에서 RNA를 분리하는 단계;1) separating RNA from test subject-derived human somatic cells and control somatic cells;
2) 단계 1)의 실험군 및 대조군의 RNA를 cDNA로 합성하면서 실험군과 대조군 을 각기 다른 형광물질로 표지하는 단계;2) labeling the experimental group and the control group with different fluorescent substances while synthesizing RNA of the experimental group and the control group of step 1) with cDNA;
3) 단계 2)의 각기 다른 형광물질로 표지된 cDNA를 상기 DNA 마이크로어레이칩과 혼성화 시키는 단계;3) hybridizing cDNA labeled with different fluorescent materials of step 2) with the DNA microarray chip;
4) 단계 3)의 반응한 DNA 마이크로어레이칩을 분석하는 단계; 및4) analyzing the reacted DNA microarray chip of step 3); And
5) 단계 4)의 분석한 데이터에서 본 발명의 바이오마커의 발현 정도를 대조군과 비교하여 확인하는 단계를 포함하는, 헥사클로로벤젠에 대한 노출 여부를 확인하기 위해 상기 바이오마커를 검출하는 방법을 제공한다.5) providing a method for detecting the biomarker to confirm the exposure to hexachlorobenzene, comprising the step of confirming the expression level of the biomarker of the present invention in the analyzed data of step 4) compared to the control group do.
상기 방법에 있어서, 단계 1)의 체세포는 인간 간암 세포주(HepG2)를 사용하는 것이 바람직하나 이에 한정되는 것은 아니며, 인간의 간 또는 간암의 세포 및 조직에서 유래한 세포라면 모두 사용 가능하다. 이때, 대조군은 건강한 개체일 것이다.In the above method, the somatic cell of step 1) preferably uses human liver cancer cell line (HepG2), but is not limited thereto, and any cell derived from cells and tissues of human liver or liver cancer may be used. At this time, the control group will be a healthy individual.
상기 방법에 있어서, 단계 2)의 형광물질은 Cy3, Cy5, FITC(poly L-lysine-fluorescein isothiocyanate), RITC(rhodamine-B-isothiocyanate) 및 로다민(rhodamine)으로 이루어진 군으로부터 선택되는 것이 바람직하나 이에 한정되는 것은 아니며, 당업자에게 알려진 형광물질은 모두 사용 가능하다. In the method, the fluorescent material of step 2) is preferably selected from the group consisting of Cy3, Cy5, poly L-lysine-fluorescein isothiocyanate (FITC), rhodamine-B-isothiocyanate (RITC), and rhodamine (rhodamine). It is not limited to this, any fluorescent material known to those skilled in the art can be used.
상기 방법에 있어서, 단계 3)의 혼성화는 40 내지 50℃에서 10 내지 20시간 동안 수행하는 것이 바람직하나 이에 한정되는 것은 아니다.In the above method, the hybridization of step 3) is preferably performed at 40 to 50 ° C. for 10 to 20 hours, but is not limited thereto.
상기 방법에 있어서, 단계 3)의 DNA 마이크로어레이 칩은 인간 게놈 중 본 발명에서 상기 과발현 또는 저발현 유전자(표 2 및 표 3 참조)가 탑재된 마이크로어레이 칩이라면 사용 가능하고, Whole Human Genome Oligo Microarray(Agilent, USA) 등을 사용하는 것이 바람직하나 이에 한정되는 것은 아니며, 상기 본 발명자가 제작한 DNA 마이크로어레이 칩을 사용하는 것이 가장 바람직하다.In the above method, the DNA microarray chip of step 3) can be used as long as it is a microarray chip loaded with the over- or low-expression gene (see Tables 2 and 3) in the human genome of the human genome, and Whole Human Genome Oligo Microarray. (Agilent, USA) and the like are preferably used, but are not limited thereto. It is most preferable to use the DNA microarray chips produced by the present inventors.
상기 방법에 있어서, 단계 4)의 분석은 혼성화된 마이크로어레이 칩을 세척 및 염색한 후 스캔하여 이미지 프로세싱을 수행한 다음 통계적 분석을 수행하는 것이 바람직하다. 상기 염색 및 세척은 uidics Station 450을 이용하고, 스캔은 GeneChip scanner 3000을 이용하며, 이미지 프로세싱은 Affymetrix GeneChip Operating System(GCOS)을 이용하며, 모든 절차는 Affymetrix사의 프로토콜에 따라 수행하는 것이 바람직하나 이에 한정되지 않는다. 상기 통계학적 분석은 GenPlex software version 2.3을 이용하여 수행하는 것이 바람직하나 이에 한정되는 것은 아니며, 당업자에게 알려진 분석 소프트웨어를 사용하여도 무방하다.In the above method, the analysis of step 4) is preferably performed by washing and staining the hybridized microarray chip, scanning, performing image processing, and then performing statistical analysis. The staining and washing using uidics Station 450, the scan is GeneChip scanner 3000, and the image processing is Affymetrix GeneChip Operating System (GCOS), and all procedures are preferably performed according to the protocol of Affymetrix, but is not limited thereto. The statistical analysis is preferably performed using GenPlex software version 2.3, but is not limited thereto. Analysis software known to those skilled in the art may be used.
또한, 본 발명은In addition,
1) 실험군인 피검체 유래 인간 체세포와 대조군 체세포에서 RNA를 분리하는 단계;1) separating RNA from test subject-derived human somatic cells and control somatic cells;
2) 단계 1)의 RNA를 대상으로, 본 발명의 바이오마커 유전자를 증폭할 수 있는 프라이머쌍을 사용하여 실시간 RT-PCR(Real-time reverse transcript polymerase chain reaction)을 수행하는 단계; 및2) performing real-time reverse transcript polymerase chain reaction (RT-PCR) on the RNA of step 1) using a primer pair capable of amplifying the biomarker gene of the present invention; And
3) 단계 2)의 유전자 산물을 대조군과 비교하여 발현 정도를 확인하는 단계를 포함하는, 헥사클로로벤젠에 대한 노출 여부를 확인하기 위해 상기 바이오마커 를 검출하는 방법을 제공한다.3) it provides a method of detecting the biomarker to determine the exposure to hexachlorobenzene comprising the step of confirming the expression level by comparing the gene product of step 2) with the control.
상기 방법에 있어서, 단계 1)의 체세포는 인간 간암 세포주(HepG2)를 사용하는 것이 바람직하나 이에 한정되는 것은 아니며, 인간의 간 또는 간암의 세포 및 조직에서 유래한 세포라면 모두 사용 가능하다.In the above method, the somatic cell of step 1) preferably uses human liver cancer cell line (HepG2), but is not limited thereto, and any cell derived from cells and tissues of human liver or liver cancer may be used.
상기 방법에 있어서, 단계 3)의 프라이머 쌍은 단계 1)의 프라이머 쌍은 바이오마커 유전자의 증폭 산물이 100 내지 300 bp가 되도록 설계된 15 내지 50머 길이, 바람직하게는 15 내지 30머의 길의, 더욱 바람직하게는 18 내지 25머의 길이의 정방향 및 역방향 프라이머 쌍은 모두 사용 가능하다.In the method, the primer pair of step 3) is 15-50mers long, preferably 15-30mers long, designed such that the amplification product of the biomarker gene is 100-300 bp, More preferably both forward and reverse primer pairs of length 18 to 25 mer can be used.
또한, 본 발명은 상기 마이크로어레이 칩을 포함하는, 헥사클로로벤젠에 대한 노출 여부 확인용 키트를 제공한다.In addition, the present invention provides a kit for confirming exposure to hexachlorobenzene, including the microarray chip.
상기 확인용 키트에 추가적으로 인간 체세포를 포함하는 헥사클로로벤젠에 대한 노출 여부 진단용 키트를 제공한다. 상기 인간 간세포는 HepG2를 사용하는 것이 바람직하나 이에 한정되는 것은 아니며, 인간의 간 또는 간암의 세포 및 조직에서 유래한 세포라면 모두 사용 가능하다. In addition to the identification kit, a kit for diagnosing exposure to hexachlorobenzene including human somatic cells is provided. The human hepatocyte is preferably HepG2, but is not limited thereto. Any human hepatocyte may be used as long as it is derived from cells and tissues of human liver or liver cancer.
상기 키트에 추가적으로 형광물질을 포함할 수 있으며, 상기 형광물질은 스트렙아비딘-알칼리 탈인화효소 접합물질(strepavidin-like phosphatease conjugate), 화학형광물질(chemiflurorensce) 및 화학발광물질(chemiluminescent)로 이루어진 군으로부터 선택되는 것이 바람직하나 이에 한정되는 것은 아니다.The kit may further include a fluorescent material, wherein the fluorescent material is selected from the group consisting of a strepavidin-like phosphatease conjugate, a chemiflurorensce and a chemiluminescent material. It is preferred to be selected, but is not limited thereto.
상기 키트에 추가적으로 반응 시약을 포함할 수 있으며, 상기 반응 시약은 혼성화에 사용되는 완충용액, RNA로부터 cDNA를 합성하기 위한 역전사효소, cNTPs 및 rNTP(사전 혼합형 또는 분리 공급형), 형광 염색제의 화학적 유도제와 같은 표식시약, 세척 완충용액 등으로 구성될 수 있으나 이에 한정된 것은 아니며, 당업자에게 알려진 DNA 마이크로어레이 칩의 혼성화 반응에 필요한 반응 시약은 모두 포함할 수 있다.The kit may further include a reaction reagent, which is a buffer used for hybridization, reverse transcriptase for synthesizing cDNA from RNA, cNTPs and rNTP (premixed or separated feed), chemical inducer of fluorescent dyes It may be composed of a labeling reagent, such as a washing buffer, but is not limited thereto, and may include all reaction reagents required for hybridization of DNA microarray chips known to those skilled in the art.
아울러, 본 발명은 상기 바이오마커 유전자에 상보적이고 바이오마커 유전자를 증폭할 수 있는 프라이머 쌍을 포함하는, 헥사클로로벤젠에 대한 노출 여부 확인용 키트를 제공한다.In addition, the present invention provides a kit for confirming exposure to hexachlorobenzene, comprising a primer pair complementary to the biomarker gene and capable of amplifying the biomarker gene.
상기 확인용 키트의 프라이머 쌍은 다음과 같은 서열번호 1 내지 11로 기재되는 서열로 구성된 군으로부터 선택되어 사용하는 것이 바람직하나 이에 한정되는 것은 아니며, 상기 바이오마커 유전자의 증폭 산물이 100 내지 300 bp가 되도록 설계된 15 내지 50머 길이, 바람직하게는 15 내지 30머의 길의, 더욱 바람직하게는 18 내지 25머의 길이의 정방향 및 역방향 프라이머 쌍은 모두 사용 가능하다.Primer pair of the identification kit is preferably used selected from the group consisting of the sequence described in SEQ ID NO: 1 to 11, but is not limited thereto, the amplification product of the biomarker gene is 100 to 300 bp Both forward and reverse primer pairs designed to be 15-50 mer long, preferably 15-30 mer long, more preferably 18-25 mer long, are available.
상기 확인용 키트에 추가적으로 인간 체세포를 포함하는 헥사클로로벤젠에 대한 노출 여부 진단용 키트를 제공한다. 상기 인간 간세포는 HepG2를 사용하는 것이 바람직하나 이에 한정되는 것은 아니며, 인간의 간 또는 간암의 세포 및 조직에서 유래한 세포라면 모두 사용 가능하다. In addition to the identification kit, a kit for diagnosing exposure to hexachlorobenzene including human somatic cells is provided. The human hepatocyte is preferably HepG2, but is not limited thereto. Any human hepatocyte may be used as long as it is derived from cells and tissues of human liver or liver cancer.
상기 키트에 추가적으로 반응 시약을 포함할 수 있으며, 상기 반응 시약은 RNA로부터 cDNA를 합성하기 위한 역전사효소, cNTPs 및 rNTP(사전 혼합형 또는 분 리 공급형), 형광 염색제의 화학적 유도제와 같은 표식시약, 세척 완충용액 등으로 구성될 수 있으나 이에 한정된 것은 아니며, 당업자에게 알려진 RT-PCR 반응에 필요한 반응 시약은 모두 포함할 수 있다.In addition to the kit may include a reaction reagent, the reagent, such as reverse transcriptase for synthesizing cDNA from RNA, cNTPs and rNTP (premixed or separated feed), chemical inducer of fluorescent dyes, washing It may be composed of a buffer solution and the like, but is not limited thereto, and may include all reaction reagents required for the RT-PCR reaction known to those skilled in the art.
이하, 본 발명을 실시예에 의해 상세히 설명한다.Hereinafter, the present invention will be described in detail with reference to examples.
단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다.However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.
<실시예 1> 세포 배양 및 화학물질 처리≪ Example 1 > Cell culture and chemical treatment
<1-1> 세포배양<1-1> Cell culture
인간 간암 세포주인 HepG2 세포(Human hepatocellular liver carcinoma cell line)를 5% FBS가 첨가된 DMEM 배지(Gibro-BRL, USA)를 이용하여 100 ㎜ 디쉬(dish)에서 80% 정도 자랄 때까지 배양하였다. 본 발명자들은 기존의 연구와 보고를 통해 잔류성 유기 오염물질 중 하나인 헥사클로로벤젠을 선정하였으며, 이를 DMSO에 용해시켰다. 매질(vehicle) 농도는 모든 실험에서 0.1% 이하였다.Human liver cancer cell line HepG2 cells (Human hepatocellular liver carcinoma cell line) were incubated in DMEM medium (Gibro-BRL, USA) to which 5% FBS was added until 80% growth in 100 mm dish. The inventors have selected hexachlorobenzene, one of the persistent organic pollutants, through existing studies and reports, and dissolved it in DMSO. Vehicle concentration was less than 0.1% in all experiments.
<1-2> 세포 독성 실험(MTT assay) 및 화학 물질 처리<1-2> Cytotoxicity test (MTT assay) and chemical treatment
Mossman 등(J. Immunol. Methods, 65:55-63, 1983)의 방법으로 HepG2 세포주 를 이용한 MTT 실험을 수행하였다. 24-웰 플레이트의 로 DMEM 배지에 80 × 104/웰 세포수로 분주하여 DMSO에 용해된 헥사클로로벤젠을 처리하였고, 48시간 후에 MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetra zolium bromide) 5 ㎎/㎖을 혼합하여 튜브에 가하여 37℃에서 3 시간 동안 배양하였다. 이 후 배지를 제거하고 형성된 포르마잔 크리스탈(formazan crystal)을 DMSO 500 ㎕에 용해하였다. 96-웰 플레이트로 옮겨 분주(aliquot)하엿고 흡광도 540 nm에서 O.D.값을 측정하였다. MTT experiment using HepG2 cell line was performed by the method of Mossman et al. ( J. Immunol. Methods , 65: 55-63, 1983). In a 24-well plate, raw DMEM medium was dispensed with 80 × 10 4 / well cell number to treat hexachlorobenzene dissolved in DMSO, and after 48 hours, MTT (3- [4,5-dimethylthiazol-2-yl]- 2,5-diphenyltetra zolium bromide) 5 mg / ml was mixed and incubated at 37 ° C. for 3 hours. The medium was then removed and the formazan crystal formed was dissolved in 500 μl of DMSO. The plate was transferred to a 96-well plate, aliquoted, and the OD value was measured at an absorbance of 540 nm.
HepG2 세포주에서 헥사클로로벤젠의 세포독성을 살펴본 결과, 세포가 85% 생존 농도(IC20 dose)는 80 μM 이었으며(도 1 참조), 상기 농도로 결정하여 마이크로어레이 실험을 수행하였다.As a result of examining the cytotoxicity of hexachlorobenzene in the HepG2 cell line, the 85% viable concentration (IC20 dose) of the cells was 80 μM (see FIG. 1).
<실시예 2> 마이크로어레이 실험≪ Example 2 > Microarray experiment
<2-1> RNA의 분리 및 형광 물질 표지<2-1> RNA Isolation and Fluorescent Labeling
6 × 106 세포/㎖ 농도로 100 ㎜ 디쉬에 HepG2 세포를 분주한 후, 실시예 1-2에서 결정한 농도의 헥사클로로벤젠을 48시간 동안 처리하였다. 이후, 상기 처리한 세포로부터 트리졸(trizol) 시약(Invitrogen life technologies, USA)을 사용하여 제조사의 방법대로 전체 RNA를 분리하였고, RNeasy mini kit(Qiagen, USA)를 사용하여 정제하였다. 게놈 DNA는 RNA 정제 동안 RNase-free DNase set(Qiagen, USA)를 사용하여 제거하였다. 각 전체 RNA 시료의 양은 분광광도계로 측정하였고, 농도는 Experion(Automated Electrophoresis station, Bio-Rad, USA)으로 확인하였다.After dispensing HepG2 cells in a 100 mm dish at a concentration of 6 × 10 6 cells / ml, hexachlorobenzene at the concentration determined in Example 1-2 was treated for 48 hours. Thereafter, total RNA was isolated from the treated cells using a trizol reagent (Invitrogen life technologies, USA) according to the manufacturer's method, and purified using an RNeasy mini kit (Qiagen, USA). Genomic DNA was removed using an RNase-free DNase set (Qiagen, USA) during RNA purification. The amount of each total RNA sample was measured by spectrophotometer, the concentration was confirmed by Experion (Automated Electrophoresis station, Bio-Rad, USA).
<2-2> 표지된 cDNA 제조 <2-2> Labeled cDNA Preparation
올리고 마이크로어레이 분석을 위하여 실시예 2-1에서 수득한 헥사클로로벤젠 처리군의 전체 RNA를 사용하여 cDNA를 제조하였다. 구체적으로, 상기 수득한 전체 RNA 30 ㎍과 올리고(dT) 프라이머 2 ㎍(1 ㎍/㎕)를 혼합하고 65℃에서 10분간 반응시킨 후 바로 얼음에 넣어 어닐링(annealing)시켰다. 상기 어닐링된 RNA의 역전사(Reverse Transcript) 반응을 위해 표 1과 같이 시약을 혼합하였다.CDNA was prepared using total RNA of the hexachlorobenzene treatment group obtained in Example 2-1 for oligo microarray analysis. Specifically, 30 μg of the obtained RNA and 2 μg (1 μg / μl) of oligo (dT) primer were mixed and reacted at 65 ° C. for 10 minutes, and then annealed in ice immediately. Reagents were mixed as shown in Table 1 for Reverse Transcript reaction of the annealed RNA.
대조군인 HepG2 세포주에서 분리한 전체 RNA는 Cy3-dUTP(녹색)로 표지화하였고, 헥사클로로벤젠을 처리한 HepG2 세포주로부터 분리한 RNA는 Cy5-dUTP(적색)로 표지화하였다. 이때 두 시료는 Microcon YM-30 컬럼(Millipore, USA)을 사용하여 혼합, 정제되었다. Total RNA isolated from the control HepG2 cell line was labeled with Cy3-dUTP (green), and RNA isolated from HepG2 cell line treated with hexachlorobenzene was labeled with Cy5-dUTP (red). At this time, the two samples were mixed and purified using a Microcon YM-30 column (Millipore, USA).
<2-3> 혼성화 반응<2-3> Hybridization reaction
혼성화 및 세척 과정은 지노첵(주)(한국)의 지시방법에 따라 수행되었다. 혼성화는 12시간 동안 62℃ 오븐에서 수행되었다. DNA 마이크로어레이 칩으로는 4X44 k Whole Human Genome Oligo Microarray(Agilent, USA)를 이용하였다. 세척 과정(2분간 2× SSC/0.1% SDS, 3분간 1× SSC 및 2분간 0.2× SSC에 세척)을 거친 후 슬라이드는 3분간 800 rpm으로 원심분리하여 건조하였다.Hybridization and washing process was carried out according to the instructions of Gino Chek Co., Ltd. (Korea). Hybridization was performed in a 62 ° C. oven for 12 hours. As a DNA microarray chip, 4 × 44 k Whole Human Genome Oligo Microarray (Agilent, USA) was used. After the washing process (washing in 2 × SSC / 0.1% SDS for 2 minutes, 1 × SSC for 3 minutes and 0.2 × SSC for 2 minutes), the slides were dried by centrifugation at 800 rpm for 3 minutes.
<2-4> 형광 이미지 획득<2-4> Fluorescence image acquisition
슬라이드 상의 혼성화 이미지는 Genepix 4000B(Axon Instruments, USA)로 스캔하였다. 결합하지 않은 유전자를 세척한 칩은 레이저 광 스캐너(laser fluorescence scanner)를 사용하여 형광 이미지를 획득하였다. 이때 녹색 형광 이미지는 대조군에서, 적색 형광 이미지는 실험군에서만 특이하게 발현되는 유전자의 활성정도를 나타내게 되며, 노란색 형광 이미지는 녹색과 적색의 보색으로 두 군의 발현이 큰 차이가 없음을 의미하였다. 스캔한 이미지들은 유전자 발현 비율을 얻기 위하여 GenePix 4.1 소프트웨어(Axon Instruments, USA)로 분석하였다. 상기 방법에 의해 수득한 데이터로부터, Cy5/Cy3 비율이 1.5배 이상인 경우 발현이 증가한 유전자로 분류하였고, 0.667배 이하인 경우 발현 감소한 유전자로 분류함으로써 헥사클로로벤젠에 대한 마커 유전자를 선별하였다. 이때, p-값(p-value)은 0.001 이하이다. Hybridization images on the slides were scanned with the Genepix 4000B (Axon Instruments, USA). Chips washed with unbound genes were obtained with a fluorescence image using a laser fluorescence scanner. In this case, the green fluorescence image in the control group, the red fluorescence image represents the activity level of the gene specifically expressed in the experimental group, and the yellow fluorescence image means that the expression of the two groups does not have a large difference in complementary colors of green and red. Scanned images were analyzed with GenePix 4.1 software (Axon Instruments, USA) to obtain gene expression rates. From the data obtained by the above method, marker genes for hexachlorobenzene were selected by classifying genes with increased expression when the Cy5 / Cy3 ratio was 1.5 times or more and by genes with reduced expression when 0.65 or less times. At this time, the p-value (p-value) is 0.001 or less.
그 결과, 발현이 증가한 유전자는 0.21%(45,220개의 유전자 중 96개), 발현이 감소한 유전자는 1.364%(45,220개의 유전자 중 617개)임을 확인하였다.As a result, it was confirmed that the gene with increased expression was 0.21% (96 of 45,220 genes) and the gene with reduced expression was 1.364% (617 of 45,220 genes).
상기 선별된 유전자들의 기능을 생물학적 방법(biological process)에 따라 분류한 결과, 표 2 및 표 3에서 나타난 바와 같이, 스트레스반응(response to stress), 번역(translation), 지혈(hemostasis), 스테로이드 대사 과정(steroid metabolic process), 면역 작용자 과정(immune effector process), 섬유소 분해(fibrinolysis), 세포 분화(cell differentiation), 세포 자살(apoptosis), 지질 대사 과정(lipid metabolic process), 전사 규칙(positive regulation of transcription), 아미노산 대사 과정(amino acid metabolic process) 또는 스테롤 대사 과정(sterol metabolic process)와 관련된 유전자로 분류되었다.As a result of categorizing the functions of the selected genes according to a biological process, as shown in Tables 2 and 3, the response to stress, translation, hemostasis, and steroid metabolic processes steroid metabolic process, immune effector process, fibrinolysis, cell differentiation, apoptosis, lipid metabolic process, transcriptional regulation of genes involved in transcription, amino acid metabolic processes, or sterol metabolic processes.
<실시예 3> 실시간 RT-PCR 정량Example 3 Real-Time RT-PCR Quantitation
상기 <실시예 2>의 헥사클로로벤젠에 의해 과발현 및 저발현된 유전자 중 몇 종의 발현변화 정도를 실시간 RT-PCR 방법으로 확인하였다.The degree of expression change of several genes overexpressed and low expressed by hexachlorobenzene of <Example 2> was confirmed by real-time RT-PCR method.
구체적으로, My IQ 실시간 PCR(My IQ Real-time PCR; Bio-rad, USA)을 이용하여 정량적인 실시간 RT-PCR을 실시하였다. 구체적으로, 올리고 dT 프라이머와 Superscript kit(Omniscipt™ kit, Qiagen, Co., USA)를 이용하여 역전사 반응을 수행하여 cDNA를 합성하였다. 먼저 PCR에 이용한 표적 유전자와 내재성(endogenous) 대조군(RPLP0)에 대한 프라이머를 사이버그린(SYBR Green) 마스터 믹스(Master mix; Bio-rad, USA)에 첨가하여 PCR을 실시한 후, 적절한 농도를 선택하는 프라이머 적합화(primer optimization) 과정을 수행하였다. 상기 cDNA 0.2 ㎕와 증류수 3.8 ㎕, 하기 표 4의 센스 프라이머 0.5 ㎕, 안티센스 프라이머 0.5 ㎕, 사이버그린 I 염색 수퍼믹스(Bio-rad, USA) 5 ㎕를 혼합하여, PCR 튜브에 담아 단계 1: 95℃, 3분; 단계 2(45회 반복): 단계 2-1: 95℃, 10초; 단계 2-2: 55 내지 65℃ 45초; 단계 3: 95℃, 1분; 단계 4: 55℃, 1분; 단계 5(반복 80회): 55℃, 10초로 프로그램을 설계한 My IQ 실시간 PCR 기계에서 반응을 수행한 후, 정량 소프트웨어(software)를 사용하여 분석하였다. 사이버그린 I 염색은 이중나선 DNA에 결합하는 염색법으로서, 실시간 RT-PCR 과정 동안 이중나선 DNA가 생성될수록 형광강도(fluroscense intensity)가 증가함으로써, 실시간 RT-PCR 산물을 정량하기 위하여 사용하였다.Specifically, quantitative real-time RT-PCR was performed using My IQ Real-time PCR (Bio-rad, USA). Specifically, cDNA was synthesized by performing reverse transcription using an oligo dT primer and a Superscript kit (Omniscipt ™ kit, Qiagen, Co., USA). First, the primers for the target gene and endogenous control (RPLP0) used for PCR were added to the SYBR Green Master mix (Bio-rad, USA), and then PCR was selected. Primer optimization was performed. 0.2 μl of the cDNA and 3.8 μl of distilled water, 0.5 μl of the sense primer of Table 4, 0.5 μl of the antisense primer, and 5 μl of the Cyberrin I staining supermix (Bio-rad, USA) were added to the PCR tube, followed by Step 1: 95. C, 3 minutes; Step 2 (repeated 45 times): Step 2-1: 95 ° C, 10 seconds; Step 2-2: 55 to 65 ° C. 45 seconds; Step 3: 95 캜, 1 min; Step 4: 55 [deg.] C, 1 min; Step 5 (80 repetitions): The reaction was performed on a My IQ real-time PCR machine designed for program at 55 ° C., 10 seconds, followed by analysis using quantification software. Cyberin I staining is a staining method that binds to double-stranded DNA, which was used to quantify real-time RT-PCR products by increasing the fluorescence intensity as the double-stranded DNA was generated during the real-time RT-PCR process.
그 결과, 하기 표 5에 나타난 바와 같이 2종의 과발현 유전자들과 1종의 저발현 유전자들의 유전자 발현 양상은 올리고 마이크로어레이 칩의 결과와 매우 유사하게 나타남을 확인하였다.As a result, as shown in Table 5, it was confirmed that the gene expression patterns of two overexpression genes and one low expression gene were very similar to those of the oligo microarray chip.
(상대적 배율)Real-time PCR
(Relative magnification)
(Cy3/Cy5 비율)cDNA Microarray
(Cy3 / Cy5 ratio)
따라서, 본 발명의 마이크로어레이 칩을 이용한 실험으로 선별된 유전자들은 헥사클로로벤젠에 대한 노출 여부 확인에 유용하게 이용될 수 있음을 알 수 있었다.Therefore, it was found that the genes selected by experiments using the microarray chip of the present invention can be usefully used to confirm exposure to hexachlorobenzene.
본 발명의 바이오마커가 집적된 DNA 마이크로어레이 칩은 헥사클로로벤젠 노출 여부를 간단하고 신속하게 판정하는데 유용하게 사용될 수 있으며, 헥사클로로벤젠에 의해 유발되는 독성 작용 기작을 규명하는 도구로 이용될 수 있다.The DNA microarray chip incorporating the biomarker of the present invention can be usefully used for simple and rapid determination of hexachlorobenzene exposure, and can be used as a tool for identifying the mechanism of toxic action caused by hexachlorobenzene. .
도 1은 헥사클로로벤젠(Hexachlorobenzene)의 인간 간암 세포주에서의 세포 독성을 조사한 그래프이다.1 is a graph illustrating the cytotoxicity of hexachlorobenzene in human liver cancer cell lines.
도 2는 헥사클로로벤젠을 처리한 인간 간암 세포주에서의 유전자 발현 양상을 마이크로어레이 칩을 이용하여 분석한 결과를 나타내는 그림이다.Figure 2 is a diagram showing the results of analyzing the gene expression pattern in the human liver cancer cell line treated with hexachlorobenzene using a microarray chip.
<110> Korea Institute of Science and Technology <120> Biomarkers for identification of exposure to Hexachlorobenzene and the method of identification using thereof <130> 9p-03-66 <160> 6 <170> KopatentIn 1.71 <210> 1 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> glutathione S-transferase A1 sense primer <400> 1 aggaagcctc ccatggatga gaaa 24 <210> 2 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> glutathione S-transferase A1 antisense primer <400> 2 ttcttggcct ccatgactgc gtta 24 <210> 3 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> kelch-like 14(Drosophila) sense primer <400> 3 acttacaaga gccacaggag ccaa 24 <210> 4 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> kelch-like 14(Drosophila) antisense primer <400> 4 actgagttca ggcatctctg gtga 24 <210> 5 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> ubiquitin-like, containing PHD and RING finger domains, 1 sense primer <400> 5 ctggtacgac gcggagat 18 <210> 6 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> ubiquitin-like, containing PHD and RING finger domains, 1 antisense primer <400> 6 cgacagtcgt tcagagaatc a 21 <110> Korea Institute of Science and Technology <120> Biomarkers for identification of exposure to Hexachlorobenzene and the method of identification using <130> 9p-03-66 <160> 6 <170> Kopatentin 1.71 <210> 1 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> glutathione S-transferase A1 sense primer <400> 1 aggaagcctc ccatggatga gaaa 24 <210> 2 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> glutathione S-transferase A1 antisense primer <400> 2 ttcttggcct ccatgactgc gtta 24 <210> 3 <211> 24 <212> DNA <213> Artificial Sequence <220> 223 kelch-like 14 (Drosophila) sense primer <400> 3 acttacaaga gccacaggag ccaa 24 <210> 4 <211> 24 <212> DNA <213> Artificial Sequence <220> 223 kelch-like 14 (Drosophila) antisense primer <400> 4 actgagttca ggcatctctg gtga 24 <210> 5 <211> 18 <212> DNA <213> Artificial Sequence <220> 223 ubiquitin-like, containing PHD and RING finger domains, 1 sense primer <400> 5 ctggtacgac gcggagat 18 <210> 6 <211> 21 <212> DNA <213> Artificial Sequence <220> 223 ubiquitin-like, containing PHD and RING finger domains, 1 antisense primer <400> 6 cgacagtcgt tcagagaatc a 21
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