KR101374186B1 - Novel bacterium decomposing PBS - Google Patents

Novel bacterium decomposing PBS Download PDF

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KR101374186B1
KR101374186B1 KR1020110065002A KR20110065002A KR101374186B1 KR 101374186 B1 KR101374186 B1 KR 101374186B1 KR 1020110065002 A KR1020110065002 A KR 1020110065002A KR 20110065002 A KR20110065002 A KR 20110065002A KR 101374186 B1 KR101374186 B1 KR 101374186B1
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pbs
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polybutylene succinate
staphylococcus
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이상준
김영옥
김경길
박성욱
김연계
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Abstract

본 발명은 해양 유래의 폴리부틸렌 숙시네이트 분해능이 있는 신규 균주 및 이를 이용한 폴리부틸렌 숙시네이트의 분해방법을 제공한다.The present invention provides a novel strain having a polybutylene succinate degrading ability derived from the ocean and a method for decomposing polybutylene succinate using the same.

Description

신규 ＰＢＳ 분해 세균{Novel bacterium decomposing PBS}Novel bacterium decomposing PBS

본 발명은 신규 세균에 관한 것으로서, 더 상세하게는 PBS 분해능을 가진 신규 균주에 관한 것이다.The present invention relates to a novel bacterium, and more particularly to a new strain having PBS resolution.

해양은 인간에게 있어 차세대 자원의 보고로서 기대를 모우고 있는 영역이라고 할 수 있다. 그러나 현재 우리 연안해역은 해양폐기물로 인해 병들어 가고 있다. 최근 국내에서 해양폐기물 중에 특히 어업인들의 어로활동을 통해 발생하는 폐어망이나 폐밧줄은 연간 수만 톤에 이르며, 이들 대부분은 바다 속으로 버려지고 있다. 이 가운데 해양생태계에 큰 악영향을 미치는 것이 어구, 어망, 로프, 발포, 컵 등의 폐플라스틱이다. 따라서 최근 자연환경에서 완전히 분해되어 사용 후 수거, 처리 단계가 불필요한 생분해성 재질로 만든 환경친화적인 어구 및 수산용 자재개발이 절실히 요구된다.The ocean is an area of high expectations for humans. But now our coastal waters are getting sick due to marine waste. In recent years, domestic marine wastes, especially fishing nets and waste ropes generated through fishing activities of fishers, reach tens of thousands of tons per year, most of which are thrown into the sea. Among these, waste plastics such as fishing gear, fishing nets, ropes, foaming, and cups have a major adverse effect on the marine ecosystem. Therefore, there is an urgent need to develop environmentally friendly fishing gear and fishery materials made of biodegradable materials that are completely decomposed in the natural environment and do not need to be collected and processed after use.

이러한 추세에 부응하기 위해 해양환경에서 완전 분해되는 생분해성 수지, 특히, 폴리부틸렌 숙시네이트{PBS, poly (butylene succinate)} 생분해성 수지로 제조된 환경친화적인 어구 및 수산용 자재의 개발이 진행중이다. PBS{poly(butylene succinate)}는 디카르복시산(dicarboxylic aicd)로서 숙신산(succinic acid)와 글리세롤(glycerol)류인 1,4-부틸렌디올(1,4-butylenediol)을 원료로 사용하여 에스테르화 반응과 축합반응을 거쳐 얻어지는 중합물로서 강도가 우수하고, 결정성이 높아 신장율이 낮은 성질을 보여 준다. 현재 진공성형, 쉬트, 불로우몰딩, 발포, 사출 등의 용도로 사용되고 있고, 어구 및 수산용 자재로도 사용되고 있다. 다만, 이러한 생분해성 수지도 정상적인 환경에서는 분해에 시간이 소요되므로, 이러한 생분해성 수지의 분해를 촉진하기 위해 이들 생분해성 수지의 분해할 수 있는 미생물의 발굴이 절실한 실정이다.In order to meet this trend, development of environmentally friendly fishing gear and fishery materials made of biodegradable resins that are completely decomposed in the marine environment, in particular, polybutylene succinate {PBS, poly (butylene succinate)} biodegradable resins is underway. to be. PBS {poly (butylene succinate)} is a dicarboxylic acid (succinic acid) and glycerol (glycerol) 1,4-butylenediol (1,4-butylenediol) as a raw material and esterification reaction The polymer obtained through the condensation reaction shows excellent strength, high crystallinity and low elongation. Currently, it is used for vacuum molding, sheet, blow molding, foaming, injection, etc., and is also used for fishing gear and fishery materials. However, since such biodegradable resins take time to decompose in a normal environment, in order to promote the decomposition of such biodegradable resins, the development of degradable microorganisms of these biodegradable resins is urgently needed.

이와 관련하여, 일본공개특허공보 제2003-310248호에는 폴리부틸렌 숙신산을 분해할 수 있는 미생물이 개시되어 있고, 일본공개특허공보 제1999-225755호에 폴리부틸렌 숙신산을 분해할 수 있는 미생물이 개시되어 있다.In this regard, Japanese Laid-Open Patent Publication No. 2003-310248 discloses a microorganism capable of decomposing polybutylene succinic acid, and Japanese Laid-Open Patent Publication No. 1999-225755 discloses a microorganism capable of decomposing polybutylene succinic acid. Is disclosed.

그러나 이러한 종래의 PBS 분해 균주는 토양 유래의 균주이기 때문에, 해양에 투기된 어구 등의 분해를 위해 사용할 수 없다는 문제점이 있었다.However, since the conventional PBS decomposition strain is a soil-derived strain, there is a problem that can not be used for decomposition of fishing gear, etc. dumped in the ocean.

본 발명은 상기와 같은 문제점을 포함하여 여러 문제점들을 해결하기 위한 것으로서, 해양에서 유래한 장치 및 방법을 제공하는 것을 목적으로 한다. 그러나 이러한 과제는 예시적인 것으로, 이에 의해 본 발명의 범위가 한정되는 것은 아니다.The present invention has been made to solve various problems including the above problems, and an object thereof is to provide an apparatus and a method derived from the ocean. However, these problems are exemplary and do not limit the scope of the present invention.

본 발명의 일 관점에 따르면, 폴리부틸렌 숙시네이트(PBS)를 분해능이 있는 Staphylococcus sp. PBS-1 균주(KACC91577P)가 제공된다. According to an aspect of the present invention, polybutylene succinate (PBS) is a Staphylococcus sp. PBS-1 strain (KACC91577P) is provided.

본 발명의 다른 관점에 따르면, 폴리부틸렌 숙시네이트(PBS)를 분해능이 있는 Paenibacillus sp. PBS-2 균주(KACC91578P)가 제공된다.According to another aspect of the invention, the polybutylene succinate (PBS) is Paenibacillus sp. PBS-2 strain (KACC91578P) is provided.

본 발명의 다른 관점에 따르면, 상기 균주를 포함하는 PBS 분해용 조성물이 제공된다.According to another aspect of the present invention, there is provided a composition for PBS degradation comprising the strain.

본 발명의 다른 관점에 따르면, 상기 균주를 PBS에 처리하는 단계를 포함하는 PBS 분해방법이 제공된다.According to another aspect of the present invention, there is provided a PBS degradation method comprising the step of treating the strain in PBS.

이 때 상기 PBS는 폐어구에 포함된 것일 수 있다.At this time, the PBS may be included in the waste gear.

상기한 바와 같이 이루어진 본 발명의 일 실시예에 따르면, 바다에 투기된 PBS 소재로 된 폐어구를 효과적으로 분해시킬 수 있다. 물론 이러한 효과에 의해 본 발명의 범위가 한정되는 것은 아니다.According to one embodiment of the present invention made as described above, it is possible to effectively decompose waste fishing gear made of PBS material dumped in the sea. Of course, the scope of the present invention is not limited by these effects.

도 1a 및 1b는 본 발명의 PBS 분해 균주들의 계통수(phylogenetic tree)를 나타낸 도면이다.
도 2는 본 발명의 PBS 분해 균주들의 PBS 함유 배지에서의 성장 곡선을 나타내는 그래프이다.
도 3a 및 3b는 탄소원으로서 PBS 다중섬유를 사용한 배지에서 본 발명의 PBS 분해 균주에 의한 PBS 분해정도를 보여주는 주사전자현미경 사진이다.1a and 1b are diagrams showing the phylogenetic tree of PBS degradation strains of the present invention.
Figure 2 is a graph showing the growth curve in PBS containing medium of PBS degradation strains of the present invention.
3a and 3b are scanning electron micrographs showing the degree of PBS degradation by the PBS degradation strain of the present invention in a medium using PBS multiple fibers as a carbon source.

이하, 첨부된 실시예를 통해 본 발명을 보다 상세히 설명하고자 한다. 그러나 본 발명은 이하에서 개시되는 실시예에 한정되는 것이 아니라 서로 다른 다양한 형태로 구현될 수 있는 것으로, 이하의 실시예는 본 발명의 개시가 완전하도록 하며, 통상의 지식을 가진 자에게 발명의 범주를 완전하게 알려주기 위해 제공되는 것이다. Hereinafter, the present invention will be described in more detail with reference to the accompanying examples. It should be understood, however, that the invention is not limited to the disclosed embodiments, but may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, Is provided to fully inform the user.

실시예 1: PBS-트립톤 한천 평판배지의 제조Example 1 Preparation of PBS-Tryptone Agar Plate Medium

Tansengco 및 Tokiwa에 의해 언급된 바(Tansengco and Tokiwa, 1998. World Journal of Microbiology & Biotechnology, 14, 133-138)에 기반한 기본배지[(NH4)2SO4 1 g, MgSO4·7H2O 200 mg, NaCl 100 mg, CaCl2·2H2O 20 mg, FeSO4·7H2O 10 mg, Na2MoO4·2H2O 0.5 mg, Na2WO4·2H2O 0.5 mg, MnSO4 0.5 mg, K2HPO4 1.6 g, KH2PO4 200 mg]에 PBS 분말(Sigma, USA, 2 g/L)을 탄소원으로 사용하고 효모 추출물 100 mg/L 대신에 질소원으로 트립톤(100 mg/L)을 사용하여 배지를 조성하였다. 유화 배지(emulsified medium, pH 7.2)를 액체배지로 사용하였고, 한천 평판배지를 만들기 위해 20 g의 한천을 상기 유화배지에 첨가하였다. 상기 배지를 121℃에서 15분간 가압멸균기에서 멸균처리하였다. 본 발명자들은 상기 배지들을 각각 PBS-트립톤 기본배지(PBS-tryptone basal medium) 및 PBS-트립톤 기본 한천 평판배지(PBS-tryptone basal agar plate medium)로 명명하였고, 상기 배지들을 이용하여 PBS-효모 추출물-기본 한천 평판배지(PBS-yeast extract-basal agar plate, Tansengco and Tokiwa, 1998)에서 분리되지 않는 PBS 분해 균을 지시 평판배지(indicator plate)로서 분리할 수 있었다.Basic medium based on Tansengco and Tokiwa (Tansengco and Tokiwa, 1998. World Journal of Microbiology & Biotechnology, 14, 133-138) [(NH 4 ) 2 SO 4 1 g, MgSO 4 · 7H 2 O 200 mg, NaCl 100 mg, CaCl 2 · 2H 2 O 20 mg, FeSO 4 · 7H 2 O 10 mg, Na 2 MoO 4 · 2H 2 O 0.5 mg, Na 2 WO 4 · 2H 2 O 0.5 mg, MnSO 4 0.5 mg , 1.6 g of K 2 HPO 4 , 200 mg of KH 2 PO 4 ] was used as a carbon source and PBS powder (Sigma, USA, 2 g / L) and tryptone (100 mg / L) as a nitrogen source instead of 100 mg / L yeast extract. ) Was used to formulate the medium. An emulsified medium (pH 7.2) was used as a liquid medium, and 20 g of agar was added to the emulsified medium to make agar plate medium. The medium was sterilized in an autoclave at 121 ° C. for 15 minutes. The inventors named the media as PBS-tryptone basal medium and PBS-tryptone basal agar plate medium, respectively. PBS degrading bacteria that could not be separated from extract-based agar plate (PBS-yeast extract-basal agar plate, Tansengco and Tokiwa, 1998) could be isolated as indicator plates.

실시예 2: 균주의 분리 및 동정Example 2: Isolation and Identification of Strains

해양 토양 시료를 대한민국 기장군 일대에서 2009년 3월부터 10월 사이에 수집하였다. 상기 시료를 밤새 현탁시킨 후 상기 실시예 1에서 제조한 PBS-트립톤 기본 한천 평판배지 상에 도말하였다. 분리된 세균을 특정 기간동안 200 rpm의 정속으로 20℃ 및 37℃에서 각각 교반하였다. 액체배지의 시료를 성장 정도를 조사하기 위해 일정 간격으로 취해 660 nm에서의 흡광도(OD660)를 측정하였다. 각각의 실험은 세 번 반복하였고, 수득된 데이터를 이용하여 분산 분석을 수행하였다. Marine soil samples were collected from March to October 2009 in Gijang-gun, Republic of Korea. The samples were suspended overnight and then plated on a PBS-Tryptone base agar plate medium prepared in Example 1. The isolated bacteria were stirred at 20 ° C. and 37 ° C., respectively, at a constant speed of 200 rpm for a specific period of time. Samples of the liquid medium were taken at regular intervals to investigate the degree of growth, and the absorbance at 660 nm (OD 660 ) was measured. Each experiment was repeated three times and variance analysis was performed using the data obtained.

그 결과 20℃에서는 4일 배양 후 콜로니 주위에 투명한 구역을 만들어 PBS 분해 활성을 가진 1 개 균주를 분리하여 PBS-1 균주으로 명명하였고, 37℃에서는 11일 배양 후 1 개의 콜로니 주위에 투명한 구역을 만들어 PBS 분해 활성을 가진 콜로니를 분리하여 PBS-2 균주로 명명하였다. 그러나 이러한 균주들은 서로 교차된 온도에서는 용해작용을 보이지 않았다.As a result, after 4 days incubation at 20 ℃ to create a transparent zone around the colony was isolated one strain with PBS degradation activity was named PBS-1 strain, at 37 ℃ after 11 days incubated transparent zone around one colony Colonies having PBS degradation activity were isolated and named as PBS-2 strains. However, these strains did not show solubility at temperatures crossed with each other.

PBS-1 및 2 균주 각각에서 게놈 DNA를 분리한 뒤, 각 균주의 16S rRNA 서열에 대한 PCR 및 서열분석을 수행하였다(Yoon, J.-H. et al., 1998. Int. J. Syst. Bacteriol. 48, 187-194). 16S rDNA 유전자에 대한 염기서열은 하기 유니버설 프라이머를 이용하여 수행하였다(Yoon et al., 1998): 포워드 프라이머 9F(5'-GAG TTT GAT CCT GGC TCA G-3', 서열번호 1) 및 리버스 프라이머 1542R(5'-AGA AAG GAG GTG ATC CAG CC-3', 서열번호 2). 그 분석된 결과 PBS-1 균주의 16S rDNA 서열은 서열번호 3 그리고 PBS-2 균주의 16S rDNA 서열은 서열번호 4로 규명되었다. 상기 16S rDNA 서열들을 2011년 4월 3일 자로 GenBank에 각각 Acession No. JF502463 및 JF502464로 각각 등록하였다. 분석된 각 균주의 16S rRNA 서열을 EzTaxon server(//www.eztaxon.org/)를 이용하여 계통발생분석을 실시한 결과(Chun et al., 2007. Int. J. Syst. Evol. Microbiol. 57, 2259-2261), PBS-1균은 Staphylococcus epidermidis와 99.5% Staphylococcus warneri와 99.1% Staphylococcus caprae와 99%의 상동성을 갖고 있어 Staphylococcus sp. PBS-1으로 명명하였고(도 1A), PBS-2균은 Paenibacillus barcinonensis와 99%의 상동성을 가지고 있어 Paenibacillus sp. PBS-2로 명명하였으며(도 1B), 2010년 8월 23일에 각각 수탁번호 KACC91577P 및 KACC91578P로 국립농업과학원 농업유전자원센터에 기탁하였다. Genomic DNA was isolated from each of PBS-1 and 2 strains, followed by PCR and sequencing of the 16S rRNA sequences of each strain (Yoon, J.-H. et al., 1998. Int. J. Syst. Bacteriol. 48, 187-194). The base sequence for the 16S rDNA gene was performed using the following universal primers (Yoon et al., 1998): forward primer 9F (5'-GAG TTT GAT CCT GGC TCA G-3 ', SEQ ID NO: 1) and reverse primer 1542R (5'-AGA AAG GAG GTG ATC CAG CC-3 ', SEQ ID NO: 2). As a result of analysis, 16S rDNA sequence of PBS-1 strain was identified by SEQ ID NO: 3 and 16S rDNA sequence of PBS-2 strain was identified by SEQ ID NO: 4. The 16S rDNA sequences were each obtained from Acession No. It was registered as JF502463 and JF502464, respectively. As a result of phylogenetic analysis of the analyzed 16S rRNA sequence of each strain using EzTaxon server (//www.eztaxon.org/) (Chun et al ., 2007. Int. J. Syst. Evol. Microbiol . 57, 2259-2261), PBS-1 was found to have 99% homology with Staphylococcus epidermidis , 99.5% Staphylococcus warneri , 99.1% Staphylococcus caprae, and Staphylococcus sp. It was named PBS-1 (Fig. 1A), and PBS-2 had 99% homology with Paenibacillus barcinonensis, and Paenibacillus sp. It was named PBS-2 (FIG. 1B) and deposited on August 23, 2010 with the accession numbers KACC91577P and KACC91578P, respectively, at the National Institute of Agricultural Science.

실시예 3: 균주의 특성 분석Example 3: Characterization of Strains

PBS 분해 균주들의 성장곡선을 조사하기 위해 Staphylococcus sp. PBS-1 및 Paenibacillus sp. PBS-2의 각 균을 PBS-트립톤 기본배지 2 ml에 접종하고, 각각 20℃ 및 37℃에서 200 rpm의 속도로 교반하면서, 밤새 배양한 뒤, 새로운 PBS-트립톤 기본 배지 50 ml에 밤새 배양한 PBS-1 및 PBS-2 균주 배양액을 1%의 농도로 희석하여 재접종하고 각각 20℃ 및 37℃에서 200 rpm의 속도로 교반하면서 배양한 뒤, PBS-트립톤 기본 배지로 10배 희석된 배양액을 24시간 단위로 OD600 값을 측정하였다(도 2).To investigate the growth curve of PBS degrading strains, Staphylococcus sp. PBS-1 and Paenibacillus sp. Each bacterium of PBS-2 was inoculated in 2 ml of PBS-Tryptone base medium and incubated overnight at 200 rpm at 20 ° C. and 37 ° C., respectively, and then overnight in 50 ml of fresh PBS-Trypton base medium. The cultured PBS-1 and PBS-2 strain cultures were re-inoculated at a concentration of 1% and incubated with stirring at a speed of 200 rpm at 20 ° C. and 37 ° C., respectively, and then diluted 10-fold with PBS-Tryptone basal medium. The culture medium was measured for OD 600 in units of 24 hours (FIG. 2).

그 결과 Staphylococcus sp. PBS-1 균주는 20℃에서 1일 만에 최고 성장에 도달하고 바로 사멸기로 접어들어 2일에는 OD600 값이 거의 0에 수렴하여 PBS 분해능이 초기에만 매우 제한적으로 강함을 보여주었다. 반면에 Paenibacillus sp. PBS-2 균주는 1일 만에 대수기에 도달한 뒤 관찰된 4일까지 느린 성장을 계속적으로 지속하는 현상을 보였다.As a result, Staphylococcus sp. The PBS-1 strain reached its peak growth in one day at 20 ° C. and immediately entered the killing phase, and on day 2, the OD 600 value converged to almost zero, indicating that the PBS resolution was very limited only at the beginning. Paenibacillus sp. The PBS-2 strain showed a phenomena that continued to grow slowly until 4 days observed after reaching the log phase in 1 day.

따라서 이러한 성장 형태는 Staphylococcus sp. PBS-1 균주의 경우 1일 만에 PBS를 신속하게 분해함으로써 탄소원이 빠르게 고갈됨에 따라 사멸되는 것으로 보이며, 반면 분해시킨 PBS로부터 비교적 긴 생존을 보인 Paenibacillus sp. PBS-2 균주의 경우 Staphylococcus sp. PBS-1 균주와는 현저히 다른 생장곡선의 특징을 보였다. 따라서 Staphylococcus sp. PBS-1 균주는 Paenibacillus sp. PBS-2와 아주 PBS 분해능이 현저히 다른 균주임을 보여 주고 있다.Thus, these growth forms are Staphylococcus sp. In the case of the PBS-1 strain, the PBS-1 was rapidly degraded in one day, and it appeared to die as the carbon source was rapidly depleted, whereas the Paenibacillus sp. For PBS-2 strains, Staphylococcus sp. The growth curve was remarkably different from the PBS-1 strain. Thus, Staphylococcus sp. PBS-1 strain was Paenibacillus sp. PBS-2 and PBS-2 have very different resolution.

상기 결과로부터 Staphylococcus sp. PBS-1 균주는 PBS 배지를 탄소원으로서 사용할 때 비교적 짧은 기간만 생육할 수 있고, 분해된 PBS의 아주 일부만을 생장에 사용할 수 있고, 이는 PBS 분해능이 매우 제한적이거나 또는 PBS 사슬을 긴 길이로 분해하기 때문인 것으로 추정할 수 있고, 반면 Paenibacillus sp. PBS-2는 PBS 배지를 비교적 오랜 기간 사용할 수 있고, 이는 분해된 PBS를 비교적 짧은 길이 분자로 절단하여 절단된 저분자 PBS를 탄소원으로 사용할 수 있기 때문인 것으로 추정할 수 있어서, PBS를 분해하는 효소가 한 가지 만이 아니라 적어도 두 가지 이상의 여러 가지 형태가 있음을 시사한다. From the results, Staphylococcus sp. PBS-1 strains can only grow for a relatively short period of time when using PBS medium as a carbon source, and only a small portion of the degraded PBS can be used for growth, which is very limited in PBS resolution or to break down PBS chains into long lengths. Presumably due to Paenibacillus sp. PBS-2 can use PBS medium for a relatively long time, and it can be presumed that the cleaved PBS can be used as a carbon source by cleaving the degraded PBS into relatively short length molecules. It suggests that there are at least two different forms, not just branches.

실시예 4: PBS 분해 균들의 PBS 섬유의 분해능 조사Example 4 Investigation of PBS Fiber Resolution of PBS Degrading Bacteria

Staphylococcus sp. PBS-1 및 Paenibacillus sp. PBS-2 균주를 PBS-트립톤 기본 배지에 접종하고, 각각 20℃ 및 37℃에서 200 rpm의 속도로 교반하면서 밤새 배양한 뒤, 배양액을 탄소원인 PBS를 제거한 트립톤 기본 배지 100 ml에 1 %(v/v)으로 재접종하였다. 이때, 탄소원으로 PBS 다중섬유(PBS multi filament, (주)이래화학)를 길게 잘라서 자외선으로 5분간 쬐여 무균 처리한 후 트립톤 기본 배지 속으로 침매시킨 후 테이프로 고정시켜 사용하였다. Staphylococcus sp. PBS-1 및 Paenibacillus sp. PBS-2 균주가 접종된 배양액을 각각 20℃ 및 37℃에서 200 rpm의 속도로 교반하여 배양하면서 일주일 간격으로 PBS 다중섬유를 1 cm의 길이로 절단한 샘플을 증류수로 2회 세척 후, 37℃에서 밤새 완전히 건조시킨 후 4℃에 보관한 후, 이 PBS 다중섬유를 주사전자현미경(SAM, HITACHI S3500N)으로 1000배 배율로 촬영하여 분해정도를 조사하였다(도 3a 및 3b). Staphylococcus sp. PBS-1 and Paenibacillus sp. PBS-2 strains were inoculated into PBS-Tryptone basal medium, incubated overnight at 20 rpm and 37 ° C. at 200 rpm, and the culture medium was 1% in 100 ml of Tryptone basal medium without PBS as a carbon source. (v / v). At this time, PBS multi filament (PBS multi filament, Co., Ltd. chemicals) as a carbon source was cut long and exposed to ultraviolet light for 5 minutes, sterilized in a trypton base medium and fixed with tape. Staphylococcus sp. PBS-1 and Paenibacillus sp. After culturing the culture solution inoculated with PBS-2 strain at 20 rpm and 37 ° C. at a speed of 200 rpm, the sample cut to length PBS multi-fiber 1 cm at weekly intervals was washed twice with distilled water and then 37 ° C. After completely dried overnight at and stored at 4 ℃, the PBS multi-fiber was photographed at 1000 times magnification with a scanning electron microscope (SAM, HITACHI S3500N) to investigate the degree of degradation (Fig. 3a and 3b).

그 결과 Staphylococcus sp. PBS-1 및 Paenibacillus sp. PBS-2 균주를 1주일(도 3a) 및 2주일(도 3b) 배양한 후 적출된 PBS 다중섬유 표면에 현저한 분해흔이 관찰되었으며, 이런 결과는 대조구와 큰 차이로서, Staphylococcus sp. PBS-1 및 Paenibacillus sp. PBS-2 균주의 PBS 다중섬유에 대한 분해능이 매우 양호함이 입증되었다. As a result, Staphylococcus sp. PBS-1 and Paenibacillus sp. After incubating the PBS-2 strains for 1 week (FIG. 3A) and 2 weeks (FIG. 3B), significant degradation traces were observed on the surface of the PBS multifibers extracted. These results were significantly different from those of the control group, showing Staphylococcus sp. PBS-1 and Paenibacillus sp. It was proved that the resolution of PBS-2 strain against PBS multifiber was very good.

본 발명은 상기 실시예를 참고로 설명되었으나 이는 예시적인 것에 불과하며, 당해 기술분야에서 통상의 지식을 가진 자라면 이로부터 다양한 변형 및 균등한 다른 실시예가 가능하다는 점을 이해할 것이다. 따라서 본 발명의 진정한 기술적 보호 범위는 첨부된 특허청구범위의 기술적 사상에 의하여 정해져야 할 것이다.Although the present invention has been described with reference to the above embodiments, these are merely exemplary, and it will be understood by those skilled in the art that various modifications and equivalent other embodiments are possible. Therefore, the true technical protection scope of the present invention will be defined by the technical spirit of the appended claims.

농업생명공학연구원Agricultural Biotechnology Research Institute KACC91577PKACC91577P 2010082320100823 농업생명공학연구원Agricultural Biotechnology Research Institute KACC91578PKACC91578P 2010082320100823

서열목록 전자파일 첨부Attach an electronic file to a sequence list

Claims (5)

삭제delete 폴리부틸렌 숙시네이트 분해능이 있고, 서열번호 4로 기재되는 핵산서열로 구성되는 16S rDNA를 갖는 패니바실러스 PBS-2 균주(Paenibacillus sp. PBS-2, KACC91578P). Paenibacillus sp. PBS-2, KACC91578P, which has polybutylene succinate resolution and has 16S rDNA consisting of the nucleic acid sequence set forth in SEQ ID NO: 4. 제2항의 균주를 포함하는 폴리부틸렌 숙시네이트 분해용 조성물.Polybutylene succinate decomposition composition comprising the strain of claim 2. 제2항의 균주를 폴리부틸렌 숙시네이트에 처리하는 단계를 포함하는 폴리부틸렌 숙시네이트의 분해 방법.A process for decomposing polybutylene succinate comprising the step of treating the strain of claim 2 to polybutylene succinate. 제4항에 있어서,
상기 폴리부틸렌 숙시네이트는 폐어구에 포함되어 있는,
방법.5. The method of claim 4,
The polybutylene succinate is contained in the waste mouth,
Way.
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KR19990073601A (en) * 1999-07-28 1999-10-05 서원택 Paenibacillus sp. WN9, an extracellular microbial polysaccaride produced thereby and an additive for cement mixture containing the polysaccaride
JP2004166542A (en) * 2002-11-18 2004-06-17 Japan Science & Technology Agency New plastic decomposing bacterium
KR20070108348A (en) * 2007-10-30 2007-11-09 박세준 Sjp microorganism in bacillus

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR19990073601A (en) * 1999-07-28 1999-10-05 서원택 Paenibacillus sp. WN9, an extracellular microbial polysaccaride produced thereby and an additive for cement mixture containing the polysaccaride
JP2004166542A (en) * 2002-11-18 2004-06-17 Japan Science & Technology Agency New plastic decomposing bacterium
KR20070108348A (en) * 2007-10-30 2007-11-09 박세준 Sjp microorganism in bacillus

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