CN102242071A - Application of Pichia guilliermondii 510-6jm in treatment of petroleum hydrocarbon contaminations - Google Patents
Application of Pichia guilliermondii 510-6jm in treatment of petroleum hydrocarbon contaminations Download PDFInfo
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- CN102242071A CN102242071A CN 201110074688 CN201110074688A CN102242071A CN 102242071 A CN102242071 A CN 102242071A CN 201110074688 CN201110074688 CN 201110074688 CN 201110074688 A CN201110074688 A CN 201110074688A CN 102242071 A CN102242071 A CN 102242071A
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Abstract
The invention discloses a novel microorganism, and in particular relates to a novel microorganism Pichia guilliermondii which is capable of degrading 12C-36C linear chain alkanes. The number of the strain of the Pichia guilliermondii is 510-6jm (Pichia guillierondii sp. 510-6jm) CCTCC (China Center for Type Culture Collection) NO:M 2010173. The novel strain Pichia guilliermondii is derived from deep sea, can be used for degrading petroleum hydrocarbon substances, and is mainly applied to bioremediation and industrial washing industries. The invention discloses raw materials required for cultivating the bacterium. Bacteria cells can be cultivated by utilizing the raw materials and are used for degrading petroleum hydrocarbons.
Description
Technical field
The present invention relates to microbial technology field, specifically relate to a kind of new isolating Pichia guilliermondii (Pichia guilliermondii sp.) 510-6jm, can utilize it at the petroleum hydrocarbon class pollutant degradation function, be used for the use of repairing with the industry cleaning polluting.
Background technology
Biological restoration is the biotechnology of a class low consumption, efficient and environmental safety, mainly relies on the natural metabolism process degraded of bacterium, fungi even higher plant and cell free enzyme, removes out the pollutent in the environment.Up to now, genus surplus the microorganism of known energy degraded oil hydro carbons has 100, kind more than 200, they belong to bacterium, actinomycetes, mould and algae.The bacterium of degraded oil has Rhodopseudomonas, some bacterial strain during Pichia guilliermondii genus, Mycosphaerella, Vibrio etc. belong to.Wherein common is Rhodopseudomonas, has found that it contains the plasmid of multiple degradation of hydrocarbon, and once has been used for genetically engineered, to the degraded of short chain alkanes such as ethane, propane, butane or long chain alkane and aromatic hydrocarbons.Common degraded is the bacterial strain during the mould that has has that aspergillus, mould, a spore are mould etc. and belongs to, and white-rot fungi is very high to organic degradation efficiencies such as oil.The yeast protein that often decomposes oil in the yeast and produce with candiyeast.Find again that in recent years as if cyanobacteria and green alga degradable aromatic hydrocarbons, especially blue-green algae have the ability of the multiple aromatic hydrocarbons of oxidation.
Each state-owned nearly 80,000 ocean-going merchantman of the whole world shuttle back and forth in each harbour, the whole world at present, and they can reach more than 1,000,000 tons at the greasy dirt of term of voyage to ocean disposal every year.In addition, the marine oil overflow accident also frequently takes place in various places in recent years, marine oil pollution serious threat to the safety of the marine eco-environment.
Marine oil spill will experience dissolving, volatilization, photochemical oxidation decomposition, absorption and a series of processes such as sedimentation and biological degradation.Wherein, biological degradation is thoroughly to eliminate the most important approach of smeary, and the bioremediation technology that utilizes microbiological deterioration initiatively to remove oil spilling is current a kind of very important Environmental Biotechnology.Wherein, Pichia guilliermondii is one of oil degradation dominant bacteria important in the ocean.The present invention will provide important bacterial classification for petroleum hydrocarbon pollutes biological restoration.
Summary of the invention
The invention provides a kind of new microorganism-Pichia guilliermondii that petroleum hydrocarbon pollutes biological restoration that can be used in.Its called after Pichia guilliermondii Pichia guilliermondii sp.510-6jm, be preserved in Chinese typical culture collection center (CCTCC) on July 10th, 2010, the address: Chinese Wuhan Wuhan University, its preservation registration number is CCTCC NO:M2010173.Pichia guilliermondii of the present invention separates from the surface seawater sample, and this sample collecting is from the Indian Ocean.
Microorganism of the present invention is that 0.8-1.5 μ m is long, 0.6-1.2 μ m is wide, does not give birth to flagellum, does not produce gemma.In NaCl concentration be on 3% the LB nutrient agar 28 ℃ cultivate and can form distinctive colonial morphology in 3 days: small, oyster white, translucent, central protrusion, surface wettability, edge have irregular and transparent halo.Can utilize following carbon source: polysorbate40, tween 80, monomethyl succsinic acid, succsinic acid, γ-An Jidingsuan, seminose, melibiose.Can utilize the straight-chain paraffin of carbon chain lengths 12C～36C, growth rapidly in 16C～32C.
Table 1 is a Pichia guilliermondii physiological and biochemical property of the present invention.
Diesel oil Pichia guilliermondii of the present invention is compared and is had following feature with existing other kind that belong to together:
Table 1 physiological and biochemical property
SEQ ID No.1 is the 26S rDNA sequence D 1/D2 district of Pichia guilliermondii of the present invention, and SEQ ID No.2 is the ITS sequence of Pichia guilliermondii of the present invention.
Description of drawings
The transmission electron microscope photo of Fig. 1 diesel oil Pichia guilliermondii of the present invention.
Embodiment
Embodiment
The separation of Pichia guilliermondii 510-6jm
Gather the top layer seawater sample from the Indian Ocean, in the laboratory, can obtain Pichia guilliermondii 510-6jm of the present invention by following condition cultivation.
Artificial seawater substratum: NaCl=24g/L; MgSO
47H
2O=7.0g/L; NH
4NO
3=1g/L; KCl=0.7g/L; KH
2PO
4=2.0g/L; Na
2HPO
4=3.0g/L, pH7.4 adds an amount of micro-mixed solution after the sterilization, and mends with diesel oil as sole carbon source.Diesel oil and trace element are through 0.22 μ M membrane filtration degerming.The 100mL artificial seawater that sample is inoculated in diesel oil content 1% (v/v) is cultivated concentrated.28 ℃, 200r/min shaking table were cultivated after 4～5 days, take out 1mL and change in the 100mL fresh culture from nutrient solution.After the enrichment like this 6 times,, cultivate separation, purifying list bacterium colony after 48 hours for 28 ℃ with final enriched substance gradient dilution, spread plate.
Pichia guilliermondii 510-6jm removes the oil pollution water body
Petroleum pollution water body (pollute oil 0.5%～5%) adds inorganic nitrogen-sourced, inorganic phosphorus and bacterium liquid (inoculum size 1/10000～1/100), 1～200 liter of/ton sewage of air flow (air), and the hydrocarbon contamination clearance rate reaches 35%～40% after 7 days
Pichia guilliermondii 510-6jm removes oil pollution soil
Oil-polluted soils (polluting oil 0.5%～2%) adds nitrogenous fertilizer, phosphate fertilizer and bacterium liquid (inoculum size 1/10000～1/100), stirs ventilative 1 time in 3 days, and the after stain clearance rate reached 40%～50% in 9～12 days.
Claims (6)
1. a Pichia guilliermondii is characterized in that called after Pichia guilliermondii Pichia guilliermondii sp.510-6jm.Its preservation registration number is CCTCC M 2010173.
2. Pichia guilliermondii as claimed in claim 1 is characterized in that its 26S rDNA sequence D 1/D2 district, the ITS sequence area.
3. the application of Pichia guilliermondii as claimed in claim 1 is applied to repair the pollution that oil and related products thereof produce as one of the single component in the bioremediation agents or composition.
4. the application of Pichia guilliermondii as claimed in claim 1 is the greasy dirt on surfaces such as metal, glass and plastics is cleaned; Specifically be cleaning to workplatform, machinery equipment and component in Oil extraction and the production place, container, pipeline.
5. Pichia guilliermondii as claimed in claim 1 shakes bottle with seed culture medium and shakes seed culture medium: glucose 10 grams per liters, yeast powder 10 grams per liters, peptone 20 grams per liters.Fermention medium: glucose 10 grams per liters, yeast powder 10 grams per liters, sal epsom 4 grams per liters, Sodium phosphate dibasic 6 grams per liters, vitriolate of tartar 1 grams per liter, accent pH7.0.Seed fermentation jar 6% inoculum size, 200rpm, 30 ℃ of cultivations changed fermentor tank the same terms fermentation 36 hours in 36 hours over to.Promptly obtain the fermented liquid of Pichia guilliermondii.
6. degradation effect:
10 days degradation rates can reach 30～40% under the situation that only has inorganic nitrogen-sourced, oxygen and 1% oil (or diesel oil) to exist.
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| CN 201110074688 CN102242071B (en) | 2011-03-28 | 2011-03-28 | Application of Pichia guilliermondii 510-6jm in treatment of petroleum hydrocarbon contaminations |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103122320A (en) * | 2013-01-22 | 2013-05-29 | 浙江工业大学 | Tert-butyl 6-cyano-(3R,5R)-dihydroxyhexanoate prepared by biological catalysis and strain |
| CN106047733A (en) * | 2016-07-13 | 2016-10-26 | 华中农业大学 | Yeast strain for food fermentation, leavening agent and application of yeast strain or leavening agent |
| CN106520580A (en) * | 2016-09-05 | 2017-03-22 | 华南理工大学 | Yeast and application of yeast in catalyzing synthesis of 2,5-dihydroxymethyl furan |
| CN106591204A (en) * | 2017-01-20 | 2017-04-26 | 国家海洋局第三海洋研究所 | Microecological preparation for promoting algae growth in litopenaeus vannamei aquaculture and preparation |
| CN110283853A (en) * | 2019-07-12 | 2019-09-27 | 东北林业大学 | The method that one plant of Pichia guilliermondii generates alkane by degrading polyethylene |
| CN110317738A (en) * | 2019-07-12 | 2019-10-11 | 东北林业大学 | A method of utilizing Pichia guilliermondii Biodegradable polyethylene |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110257267A (en) * | 2019-07-12 | 2019-09-20 | 东北林业大学 | The Pichia guilliermondii of one plant of degradable polyethylene |
| CN110257268B (en) * | 2019-07-12 | 2022-05-03 | 东北林业大学 | Pichia guilliermondii strain for degrading polyethylene and producing alkane simultaneously |
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| EP1332207B1 (en) * | 2000-07-14 | 2008-10-29 | Archer-Daniels-Midland Company | Transformation systems for flavinogenic yeast |
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| EP1332207B1 (en) * | 2000-07-14 | 2008-10-29 | Archer-Daniels-Midland Company | Transformation systems for flavinogenic yeast |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103122320A (en) * | 2013-01-22 | 2013-05-29 | 浙江工业大学 | Tert-butyl 6-cyano-(3R,5R)-dihydroxyhexanoate prepared by biological catalysis and strain |
| CN106047733A (en) * | 2016-07-13 | 2016-10-26 | 华中农业大学 | Yeast strain for food fermentation, leavening agent and application of yeast strain or leavening agent |
| CN106520580A (en) * | 2016-09-05 | 2017-03-22 | 华南理工大学 | Yeast and application of yeast in catalyzing synthesis of 2,5-dihydroxymethyl furan |
| WO2018040372A1 (en) * | 2016-09-05 | 2018-03-08 | 华南理工大学 | Yeast and use thereof in catalytical synthesis of 2,5-dihydroxymethylfuran |
| CN106520580B (en) * | 2016-09-05 | 2019-08-20 | 华南理工大学 | One saccharomycete and its application in catalysis 2,5- dihydroxymethyl furans synthesis |
| US10662452B2 (en) | 2016-09-05 | 2020-05-26 | South China University Of Technology | Strain of meyerozyma guilliermondii and its use in methods of catalytic synthesis of 2,5-dihydroxymethylfuran |
| CN106591204A (en) * | 2017-01-20 | 2017-04-26 | 国家海洋局第三海洋研究所 | Microecological preparation for promoting algae growth in litopenaeus vannamei aquaculture and preparation |
| CN110283853A (en) * | 2019-07-12 | 2019-09-27 | 东北林业大学 | The method that one plant of Pichia guilliermondii generates alkane by degrading polyethylene |
| CN110317738A (en) * | 2019-07-12 | 2019-10-11 | 东北林业大学 | A method of utilizing Pichia guilliermondii Biodegradable polyethylene |
| CN110317738B (en) * | 2019-07-12 | 2022-10-28 | 东北林业大学 | Method for biodegradation of polyethylene by using pichia guilliermondii |
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| CN102242071B (en) | 2013-08-14 |
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