KR101359528B1 - Radiation protective composition comprising effective bee-venom and royal jelly - Google Patents

Abstract

The present invention relates to a radiation protection composition containing bee venom and royal jelly as an active ingredient.
The radiation protection composition of the present invention contains a bee-derived substance as an active ingredient, and in particular, the bee-derived substance includes bee venom or royal jelly or both bee venom and royal jelly.
According to this invention, the composition for radiation protection which contains bee venom and royal jelly as an active ingredient which have the outstanding radiation protection effect is provided.

Description

Radiation protection composition containing bee venom and royal jelly as active ingredient {RADIATION PROTECTIVE COMPOSITION COMPRISING EFFECTIVE BEE-VENOM AND ROYAL JELLY}

The present invention relates to a radiation protection composition containing bee venom and royal jelly as an active ingredient, and more particularly to a radiation protection composition containing bee venom and royal jelly as an active ingredient having excellent radiation protection effect.

The study on the radiation-inhibiting substance focused on the protective effect of the WR series of organic compounds containing thiols after the fact that the thiol complex containing thiols had a radioprotective effect in 1949. Because of its toxicity, practical applications have been limited. Since 1990, the United States, Israel, Japan and other chemicals such as dipyridamole, adenosine monophosphate, tetratracychlorodecaoxide, adeturon, deoxyspergualin The effectiveness of the synthesis was studied, but due to its serious toxicity, it also showed limitations in practical applications.

These radiation-induced disturbances vary with the doses exposed. In other words, during high doses (100 to 300 Gy), most of them die within a few hours to 1 to 2 days due to central nervous system disorders, and during high doses (10 to 30 Gy), gastrointestinal disorders (gastrointestinal mucosa damage) A large number of deaths occur within one to two weeks, and during intermediate doses (4 to 8 Gy), bone marrow disorders lead to hematopoietic and immune function undergrounds, causing some to die within 30 days, and low doses (1 to 2 Gy). Low dose disturbances are chronic in exposure.

In order to protect against the obstacles caused by radiation, many studies have been conducted on radiation-protective compositions that are safe for humans because of no toxicity and fewer side effects.

Korean Patent Publication No. 10-0791108 (a light absorbing fraction concentrate having a radiation protection effect and a method for manufacturing the same) discloses a composition containing a light absorbing fraction concentrate having an excellent radiation protection effect as an active ingredient.

Korean Laid-Open Patent Publication No. 10-2004-0050030 (Radiation Protection Composition) discloses a composition for radiation protection that is safe for humans, including no toxic and less side effects, including a celery extract or vitamin E.

The invention as described above only consists of studies on the production of a radiation protection composition for a variety of herbal medicines, research on the radiation protection effect on the bee-derived material has not been carried out.

Korean Registered Patent Publication No. 10-0791108 "Freshwater fraction concentrate with radiation protection effect and its manufacturing method" Korean Laid-Open Patent Publication No. 10-2004-0050030 "Composition for Radiation Protection"

An object of the present invention is to provide a radiation protection composition containing bee venom and royal jelly as an active ingredient that is safe for the human body without cytotoxicity, and also has a cell proliferation effect.

Technical problems to be achieved by the present invention are not limited to the above-mentioned technical problems, and other technical problems not mentioned above may be clearly understood by those skilled in the art from the following description. There will be.

The radiation protection composition of the present invention for achieving the above object contains a bee-derived material as an active ingredient, and in particular, the bee-derived material includes bee venom or royal jelly or bee venom and royal jelly.

The royal jelly is characterized in that it contains 100 to 1000 times the weight of the bee venom.

The radiation protection composition of the present invention is characterized by having a cell proliferation effect without cytotoxicity.

Such a radiation protection composition of the present invention may be included as an active ingredient of a pharmaceutical composition for radiation protection, or may be included as an active ingredient of a functional food for radiation protection.

Method for producing a composition for radiation protection of the present invention comprises the steps of dissolving bee venom separated from a bee with water and lyophilizing the supernatant obtained after centrifugation to prepare bee venom dried, lyophilizing the royal royal jelly to prepare a royal jelly dried And mixing the bee venom dried product with the royal jelly dried product.

In the mixing step, the royal jelly dried into 100 ~ 1000 times the weight of the bee venom dried and mixed.

According to the present invention, there is provided a radiation protection composition comprising bee venom and royal jelly as an active ingredient, which has a cell proliferation effect, and is non-toxic and has less side effects. The development can contribute to securing safety for radiation, such as reducing radiation exposure of radiation occupational workers or patients during cancer treatment.

In addition, it is possible to increase farm income by developing various products of bee venom and royal jelly.

1 is a graph showing the cell survival rate by radiation during treatment with a radiation protection composition containing bee venom and royal jelly as an active ingredient.
1: Example 1 (0.1 μg bee venom + 1 mg royal jelly)
2: Example 2 (1 microgram of bee venom + 1 mg of royal jelly)
3: Example 3 (10 μg bee venom + 1 mg royal jelly)
4: Example 4 (0.1 μg Bee Venom + Royal Jelly 10 mg)
5: Example 5 (1 μg bee venom + royal jelly 10 mg)
6: Example 6 (10 μg bee venom + Royal Jelly 10 mg)
Figure 2 is a graph showing the cell survival rate due to oxidative stress upon treatment with a radiation protection composition containing bee venom and royal jelly as an active ingredient.
1: Example 1 (0.1 μg bee venom + 1 mg royal jelly)
2: Example 2 (1 microgram of bee venom + 1 mg of royal jelly)
3: Example 3 (10 μg bee venom + 1 mg royal jelly)
4: Example 4 (0.1 μg Bee Venom + Royal Jelly 10 mg)
5: Example 5 (1 μg bee venom + royal jelly 10 mg)
6: Example 6 (10 μg bee venom + Royal Jelly 10 mg)

Hereinafter, the present invention will be described in detail.

The present invention is to provide a radiation protection composition comprising a bee-derived material as an active ingredient, the bee-derived material can be any material derived from the bee, among them bee venom or royal jelly or bee venom and royal jelly When it comprises a to prepare a composition excellent in radiation protection effect.

The bee venom, which is contained in a bee venom sac, consists of about 40 substances and has been used to treat diseases of the human body since BC.

Currently, studies on the action and mechanism of bee venom components, hypersensitivity and toxicity of bee venom, immunotherapy and arthritis, herpes simplex, multiple sclerosis, and treatment of diseases such as tumors have been reported. However, there is not much scientific research.

The royal jelly is a substance produced by bees eating a large amount of nectar and pollen, and is a nutritional substance containing protein, fat, carbohydrates and various trace elements.

Royal jelly contains a lot of vitamins and rich nutrients. Especially, acetylcholine neurotransmitter that makes women's skin moisturize, and parotin-like substance, salivary gland hormone, which prevents aging and rejuvenates muscles, bones and teeth. It is contained.

In addition, Royal Jelly contains 10-HAD, which inhibits the growth of cancer cells, and has been reported to have an excellent effect on mental anxiety, menopausal disorders, and abnormal blood pressure.

The present invention confirmed the prophylactic and therapeutic effect on cells damaged by radiation and oxidative damage by using a composition comprising a bee-derived material as described above.

That is, the composition containing bee venom 1 μg + royal jelly 10 mg showed the highest protective effect against the cytotoxicity of CHO cells by radiation, and the composition containing bee venom and royal jelly also on the cytotoxicity of CHO cells by hydrogen peroxide. (1 μg of bee venom + 10 mg of royal jelly) was found to significantly reduce cytotoxicity.

Therefore, through the development of various materials such as functional foods and pharmaceutical compositions that require radiation protection using bee venom and royal jelly, it is possible to contribute to securing safety for radiation such as reducing radiation exposure of radiation workers and patients during cancer treatment. do.

Although not shown in the following specific examples, when the radioprotective composition of the present invention is used as an active ingredient of the pharmaceutical composition as described above, a pharmaceutically acceptable carrier may be further contained, wherein the pharmaceutical Acceptable carriers are those commonly used in the preparation, such as lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpi Ralidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, and the like.

In addition, the pharmaceutical composition may further include lubricants, wetting agents, sweeteners, flavoring agents, emulsifiers, suspending agents, preservatives, and the like, in addition to the above components.

The pharmaceutical composition thus produced may be administered parenterally, or may be administered by subcutaneous infusion or topical administration through the skin (transdermal administration).

Appropriate dosages can be variously prescribed by such factors as formulation method, mode of administration, patient's age, weight, sex, morbidity, food, time of administration, route of administration, rate of excretion and response sensitivity. If administered, the content is preferably 0.001-100 mg / kg body weight per day.

The pharmaceutical compositions of the present invention may be prepared in unit dosage form by formulating with a pharmaceutically acceptable carrier and / or excipient according to methods which can be easily carried out by those skilled in the art. Or may be prepared by incorporation into a multi-dose container.

The formulations may be in the form of solutions, suspensions or emulsions in oils or aqueous media, or in the form of excipients, powders, granules, tablets or capsules, and may additionally contain dispersing or stabilizing agents.

The radiation protection composition of the present invention may be used as an active ingredient of a functional food, although not shown in the following specific examples, but includes a bee-derived material exhibiting excellent radiation protection effect by the present invention as an active ingredient. It will be apparent to those skilled in the art that the composition may be included as an active ingredient of a functional food.

The functional food, may include other ingredients that can give a synergistic effect of the main effect within the range that does not impair the main effect for the purpose of the present invention, for example, fragrances, pigments, fungicides for physical properties improvement It may further include additives such as antioxidants, preservatives, humectants, thickeners, inorganic salts, emulsifiers, synthetic polymers, and supplementary ingredients such as water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, and seaweed extracts. It may further include.

The above ingredients may be appropriately selected and blended by those skilled in the art according to the dosage form or purpose of use, and the amount may be selected within a range that does not impair the object and effect of the present invention.

In addition, the formulation of the functional food may also be in various forms such as solutions, emulsions, viscous mixtures, tablets, powders, etc., which may be ingested by various methods such as simple drinking.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples, but the scope of the present invention is not limited thereto.

<Examples 1 to 6> Preparation of the composition for radiation protection of the present invention

1) Bee Venom

The honey bee ( Apis meliffera L.) was collected by using a bee venom collecting device, and the collected bee venom was dissolved with distilled water, centrifuged, filtered, and lyophilized to separate the pure bee venom.

2) Royal Jelly

The raw solution was taken in the natural state of the raw jelly in the form of jelly in the form of jelly, lyophilized and pulverized to prepare a royal powder in the form of a dry powder.

3) Preparation of Radiation Protection Composition

To prepare a bee venom and royal jelly in the combination ratio as shown in Table 1 to carry out the following experiments.

Bee venom (BV, μg) 0.1 One 10 Royal Jelly (RJ, mg)
One Example 1 Example 2 Example 3 10 Example 4 Example 5 Example 6

Experimental Example 1 Checking Cytotoxicity of Radiation to CHO Cells

(1) Test method

 1) CHO Cell Culture and Cell Proliferation Rate

: CHO-K1 cells were purchased from Korea Cell Line Bank and used for experiments by passage in 37 ° C., 5% CO ₂ incubator using RPMI medium as ovarian-derived cells of female hamsters.

When cultured cells were grown to 90% of the floor area, subcultures were used and the cells of logarithmic growth phase were used for the experiment.

CHO-k1 cells were seeded at 5 × 10 ⁵ cells / ml in a 96 well plate, cultured for one day, attached, replaced with fresh medium, and treated with various concentrations of bee venom and royal jelly.

37 ° C, 5% CO ₂ After 24 hours of incubation, the cell viability was treated with MTT reagent (methylthiazol-2-yl-2.5-diphenyl tetrazolium bromide) and the absorbance at 540 nm was measured by ELISA reader to evaluate the cell proliferation rate of bee venom and royal jelly. It was.

(2) Test result

As a result of measuring the cytotoxicity of bee venom and royal jelly to CHO cells by MTT assay, it was confirmed that the bee venom and royal jelly content was not shown but showed no effect on cell proliferation without showing cytotoxicity. It was used in the following experiment in combination in the same ratio.

Experimental Example 2 Confirmation of Cell Viability to Radiation in CHO Cells

The compositions for Examples 1-6 were prepared and their irradiation against cell toxicity and cell viability were measured.

1) Test method

: Irradiation experiments were conducted at the university hospital. CHO-k1 cells were placed at a distance of 100 cm from the radiation source and irradiated with 2 Gy.

Before irradiation, bee venom and royal jelly were treated and incubated for 1 hour, and then irradiated.

After washing with RPMI 1640 medium 37 ℃, 5% CO ₂ After 48 hours of incubation, the resulting colonies were fixed with alcohol and stained with basic dye (crystal violet) to calculate colony forming efficiency (CFE).

2) Test result

As shown in Figure 1, the protective effect of bee venom and royal jelly composition against cytotoxicity by irradiation was measured. Treatment of bee venom and royal jelly composition was carried out concentration by 1 hour before irradiation. The radiation dose was irradiated with 2Gy selected under preliminary experimental conditions. As shown in FIG. 1, cell viability was increased by about three times in composition 4 (Example 4, bee venom 1 μg + royal jelly 10 mg).

Example 3 Measurement of Cell Viability by Oxidative Stress

1) Experimental method

: Disperse CHO-k1 cells at 5 × 10 ⁵ cells / ml, incubate for 24 hours with bee venom and royal jelly, and then expose to 0.1 mM hydrogen peroxide (H ₂ O ₂ ) for 30 minutes to reduce oxidative stress. Induced. Thereafter, the cells were exchanged with normal medium of RPMI 1640 for 48 hours, and then cell viability was analyzed by MTT.

2) Experimental results

H ₂ O ₂ on cells When treated with bee venom and royal jelly composition as shown in Figure 2 24 hours before the treatment was confirmed a cytoprotective effect.

That is, the regeneration of cells damaged by radiation and oxidative stress was most effective in the composition of Example 5 of bee venom and royal jelly. When treated with bee venom alone, the effect was very weak at below 0.1 or above 10, but when royal jelly was treated together, it was confirmed to have a synergistic effect. In addition, in the case of royal jelly, the radiation blocking effect was not confirmed at 1 mg or less, but in combination with bee venom, there was a radiation blocking effect. In other words, the treatment with bee venom and royal jelly alone was effective, but the effect was weak, when the combination of the two were confirmed that the effect is significantly increased.

Based on the above results, the present invention provides a radiation protection composition comprising a bee venom and royal jelly as an active ingredient, which has a cell proliferation effect, and is nontoxic and has low side effects, which is safe for humans. Through the development of various materials, such as drug development, it can contribute to securing radiation safety, such as reducing the radiation exposure of radiation occupational workers or patients during cancer treatment, and can also increase farm income by developing various products of bee venom and royal jelly. It can be seen.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. You can implement the examples. The scope of the present invention is defined by the appended claims, and all differences within the scope of the claims are to be construed as being included in the present invention.

Claims (11)

delete delete delete delete delete delete delete delete Collecting bee venom from bees by a bee venom collecting device from a Western bee of Apis meliffera L., dissolving the bee venom with distilled water, centrifuging and preparing a bee venom dried bean by freeze-drying the supernatant;
Preparing a royal jelly dried product by lyophilizing the raw royal jelly; And
Mixing the bee venom dried product with the royal jelly dried, and adding and mixing 10 mg of the royal jelly dried per 1 μg of the bee venom dried,
Method for producing a composition for radiation protection.
delete Prepared by the manufacturing method of claim 9,
Characterized by containing 10 mg of the royal jelly dried per 1 μg of the bee venom dried from the Western bee of Apis meliffera L.,
Radiation protection composition.

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