KR101358718B1 - Cosmetic composition which has ricinus communis l. extract and sophora japonica flower extract as active components, and improves cellulite - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a castor extract and a lump extract as an active ingredient and a cosmetic composition comprising isoquacitrine. The cosmetic composition of the present invention has an excellent effect on the improvement of cellulite and body slimming.

Description

COSMETIC COMPOSITION WHICH HAS RICINUS COMMUNIS L. EXTRACT AND SOPHORA JAPONICA FLOWER EXTRACT AS ACTIVE COMPONENTS, AND IMPROVES CELLULITE}

The present invention relates to a cosmetic composition having a cellulite improving effect and a slimming effect and a manufacturing method thereof.

Recently, as interests and expectations for skin care have increased due to improved living standards, women's interest in cosmetics, especially functional beauty products, has been increasing day by day and interest in slimming products that give special functions is also increasing.

Cellulite occurs mainly in women's skin and subcutaneous fat, and is a phenomenon that becomes rough skin like rough orange peel due to circulatory disorder due to accumulation of excess fat and waste products. The cause of cellulite is different from mere obesity, but it is basically caused by the increase or hypertrophy of fat cells, so the breakdown and release of fat in fat cells can ultimately help to create a slim and smooth body.

The present inventors, while studying the cosmetic composition having a cellulite improvement and body slimming effect, confirmed that the cellulite improvement and body slimming effect is good when applying a specific plant extract or isoquacitrin to the skin and completed the present invention.

An object of the present invention is to provide a cosmetic composition having a cellulite improving effect and a slimming effect.

In order to achieve the above object, the present invention provides a cosmetic composition comprising the castor extract and lump extract as an active ingredient.

In another aspect, the present invention provides a cosmetic composition comprising isoquacitrin as an active ingredient.

Cosmetic composition of the present invention has a cellulite improvement and a slimming effect shows excellent cosmetic beauty.

1 is a result of measuring the adipocyte inhibitory ability of flavonoids.
Figure 2 is a result confirming the Wnt / β-catenin signaling promoting ability of the flavonoids using HEK 293-TOP cells.
Figure 3 shows the results of confirming the Wnt / β-catenin signaling promoting ability of the flavonoids using 3T3-L1 cells.
4 is a result confirming the Wnt / β-catenin signaling promoting ability of the extract of the plant extracts.

The present invention includes a specific plant extract as an active ingredient, provides a cosmetic composition having an effect of improving cellulite.

In another aspect, the present invention provides a cosmetic composition comprising isoquacitrin, and has a cellulite improving effect.

In another aspect, the present invention provides a method for producing a cosmetic composition having a cellulite improving effect comprising the step of adding isoquacitrin to the cosmetic base.

The present invention also provides a method for producing a cosmetic composition having a cellulite improving effect comprising the step of adding a plant extract to the cosmetic base.

Hereinafter, the present invention will be described in detail.

The cosmetic composition of the present invention is applied to the skin and used. Preferably the cosmetic composition of the present invention is a cosmetic composition for transdermal absorption.

The cosmetic composition of the present invention may include a plant extract, the plant is castor, lumps, bark wood, cypress, 칡, glycolysis, dried geolji, jimo, short axis, haegeum. Preferably the plant of the invention is castor and / or lumped.

The plant extract of the present invention may use the outpost of the plant or the plant may be used for each part. For example, the leaves, stems, stem bark (bark) of the plant, wood, fruit, root, ground, underground, outpost can be used.

The plant extract of the present invention can be prepared by hot water extraction or extraction with a lower alcohol having 3 or less carbon atoms. The lower alcohol having 3 or less carbon atoms is preferably methanol, but is not limited thereto.

The cosmetic composition of the present invention has cellulite improving effect. The cellulite improvement effect refers to a decrease in the volume of the cellulite, a decrease in the height of the cellulite, a removal of cellulite, and a flattening of the cellulite and its surroundings.

The cosmetic composition of the present invention has an effect of smooth skin. This is an effect of inhibiting and preventing the skin from becoming uneven due to aggregation of subcutaneous adipocytes and surrounding waste water and moisture, thereby preventing cellulite from being formed.

Meanwhile, the cosmetic composition of the present invention has a body slimming effect. Body slimming refers to promoting the redistribution of fat evenly in the bumpy body line that is not uniformly distributed under the skin, reducing the volume of the body or part of the body, Reducing the volume of a portion of the fat cells, improving localized obesity, dissolving fat in adipocytes, reducing triglycerides and body fluid accumulation in adipocytes, and the like.

In addition, the cosmetic composition of the present invention is effective in improving skin elasticity, and has an effect of improving obesity.

The cosmetic composition of the present invention can be applied to both men's cosmetics and women's cosmetics. However, considering that cellulite is well-tolerated by women, it is useful to use the cosmetic composition of the present invention as a cosmetic composition for women. The cosmetic composition of the present invention may also be used to prevent the development of cellulite due to hormonal changes in the pregnant woman.

The cosmetic composition of the present invention may be prepared in any formulations conventionally produced in the art and may be formulated into a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant- containing cleansing oil, powder foundation , An emulsion foundation, a wax foundation and a spray, but is not limited thereto. The cosmetic composition of the present invention may also be formulated as a softening agent, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

In the cosmetic composition of the present invention, the skin absorption rate of the active ingredient is 0.01 μg / cm ² to 150 μg / cm ² per hour. Preferably the cosmetic composition of the present invention has a skin absorption rate of 0.1 μg / cm ² to 100 μg / cm ² per hour, more preferably 1.0 μg / cm ² to 50 μg / cm ² per hour. When the skin absorption rate is less than 0.01 μg / cm ² per hour, the cellulite improvement effect is insignificant, and when the skin absorption rate exceeds 150 μg / cm ² per hour, the cellulite improving ability / body slimming effect is increased compared to the active ingredient dose. Inadequate and uneconomical The active ingredient is a plant extract, preferably isoquacitrin.

The cosmetic composition of the present invention has a high isoquacitrine content as compared to a general cosmetic composition.

Isoquacitrin of the present invention may be added to cosmetic compositions in the form of a compound, i.e., a compound of formula (1), a derivative thereof or a salt thereof. In addition, the isoquacitrine of the present invention may be added in the cosmetic composition in the form of an extract containing isoquacitrin. The extract may be a plant extract. That is, the plant extract may be one or more extracts selected from the group consisting of castor extract, lump extract, bark tree extract, cypress extract, 칡 extract, glycolysis extract, dried huangji extract, hairy extract, shrunken extract and haegeum extract.

The cosmetic composition of the present invention may contain a skin penetration promoter for effective transdermal absorption. The skin penetration promoting agent is not particularly limited so long as it is a general skin penetration promoting agent used in the cosmetic composition in the art. For example, the skin penetration promoter of the present invention may be a fatty acid / ester compound such as dimethylsulfoxide, oleic acid, limonene, and the like.

When the cosmetic composition of the present invention is used, transdermal absorption may be promoted by using physical methods such as shock waves, iontophoresis, electrical invasion, and microinfiltration.

Hereinafter, the present invention will be described in more detail with reference to the following examples and experimental examples. However, the following examples and experimental examples are intended to illustrate the contents of the present invention, but the scope of the invention is not limited by the examples and the experimental examples.

<Production of Plant Extracts>

The leaves and stems of the bark tree, the bark and stems of the cypress tree, the bark of the larvae, the stem of persimmon, the castor stem, and the outpost, respectively, were dried (shape, room temperature, about 5 days) and then ground. 200 ml of methanol was added to these 30-40 g, methanol extracted (HPLC Methanol, 50 ° C, 1500psi, 20 minutes, DIONEX Corporation, ASE300 ACCELERATED SOLVENT EXTRACOR), and the solution was dispensed into a sample tube and concentrated to Biotron corporation, Modul spin 40 ( 45 ℃, reduced pressure, 24 hours) and then extracted samples were stored at a low temperature.

On the other hand, Guanjihwang, lumps, jimo, uniaxial and Haegeumsa dried outposts (grain, room temperature, about 5 days), and then crushed. 2 L of water was added to 0.1 kg of these milled samples, followed by hot water extraction using a yaktanggi (Daewoong yaktanggi) for 2.5 hours, filtered and freeze-dried for 12 hours (Biotron corporation, Vaccum free dryer cleanvac 8).

Preparation of Examples 1 to 7

To prepare a cosmetic base having the ingredients of Table 1 below. And 1.0 weight part of components of Table 2 were mixed with respect to 100 weight part of purified water of Table 1, and the cosmetic composition sample was produced (Examples 1-7). For example, Example 1 adds 1.0 part by weight of castor starch extract to the cosmetic base of Table 1, and Example 2 adds 1.0 part by weight of lump extract to the cosmetic base of Table 1. In addition, Example 3 is a total of 1.0 parts by weight of the castor extract and lump extract in the same amount to the cosmetic base of Table 1 added. At this time, the plant extracts were used as extracts prepared in the <preparation of plant extracts>.

Experimental Example 1 Measurement of Adipocyte Inhibitory Activity

Human precursor fat cell line 3T3-L1 cells (Korea Cell Line Bank) were treated with DMEM (Dulbeco's Modified Eagle's Medium) containing 10% fetal bovine serum (FBS) and streptomycin at 37 ° C and 5% CO. _The cells were cultured in an incubator maintained at ₂ . When cells became confluent, they were subcultured with trypsin-EDTA. In order to induce differentiation of the cells, a suspension solution was prepared so as to have a cell density of 3.3 × 10 ³ cells / cm 2, and the cells were second cultured by dispensing into 12 well plates. When cells became confluent, differentiation was induced by exchanging with 10% FBS-DMEM medium containing 5 ug / ml insulin, 0.25 Μm dexamethasone zone, and 0.5 mM IBMX (isobutylmethylxanthine). About 10 days after the differentiation medium treatment, more than 90% of the cells differentiate into adipocytes.

Differentiated adipocytes were incubated in 60 mm dish for 24 hours at 1x10 ⁶ cells / well, washed once with HBSS (Hanks' balanced salt solution), exchanged with medium without FBS, Rutin, Quercetin , Isoquercitrin and Isohamnetin were each added 50 uM and reacted for 24 hours. After the reaction for 24 hours, the medium was removed, washed twice with PBS, and then, 1 ml of PBS containing 10% formaldehyde, which had been made cold, was fixed for 1 hour, and washed three times with PBS. After adding 0.7% Oil red O solution (Muto Chemical, Tokyo. Japan) and reacting for 2 hours, the cells were observed. At this time, the cells were stained red.

As a result, it was confirmed that the fat was reduced the group to which isoquacitrine was added (FIG. 1).

Experimental Example 2 Flavonoids Promote Wnt / β-catenin Signaling

HEK 293-TOP cells (3 × 10 4) were seeded in 96-well plates and incubated in DMEM containing 10% FBS for one day. As a sample to cells, Hyroside, Rutin, Quercetin, Isoquercitrin, Isorhamnetin, Hyperin, Quercitrin Hydrate, 10 μM and 50 μM of Daidzein, Genistein, and tannic acid, respectively, were incubated for 24 hours, and then total cell lysate was 25 μL 1Ⅹ reporter lysis buffer per well (Promega, Madison, WI) was obtained by lysis. A sample untreated group was used as a control. Reporter activity was measured using a microplate luminometer (BMG Labtech, Offenburg, Germany) with 25 μL of luciferin (USB, Cleveland, OH) per well.

As a result, 1mM VPA, an activator of the Wnt / b-catenin pathway, greatly increased the reporter activity, and didzeyne, Zenithine and isoquacitrine were effective, and isoquacitrine showed the highest effect (FIG. 2). .

Experimental Example 3 Flavonoids Promote Wnt / β-catenin Signaling

3T3-L1 cells were fixed for 15 minutes at RT with 4% PFA. Cells were treated with 0.1% Triton X-100 for 15 minutes at RT for permeabilization. Cells were blocked with 5% BSA, 1% NGS for 30 min at RT, and incubated overnight at 4 ° C. with mouse anti-β-catenin antibody (BD transduction laboratory, 1: 100). The cells were washed with PBS and then double stained with Alexa 488-conjugated anti-mouse antibody for 1 hour at RT. After counterstaining with DAPI and mounting, signals were detected with a confocal microscope (LSM510 META). Cells were treated with 50 μM of VPA (Valproic acid), Rutin, Quercetin, Isoquercitrin, and Isohamnetin, respectively, and 48 hours later, β-catenin level was measured through immunocytochemistry. Confirmed. On the other hand, a control group, that is, a positive control group was used LiCl treatment group well known as an activator of the Wnt / β-catenin pathway.

As a result, β-catenin level was increased in nucleus and cytosol. Also, among the treated flavonoids, isoquacitrine had the highest β-catenin level (FIG. 3).

Experimental Example 4 Wnt / β-catenin Signaling Promoting Activity of the Extract

HEK 293-TOP cells (3 × 10 ⁴ ) were seeded in 96-well plates and incubated in DMEM containing 10% FBS for one day. As a sample to the cells, the bark (leaf, stem), cypress (bark), cypress (stem), 칡 (bark), castor (stem), castor (outpost), corresponding to the production of the extract (Stem), dried Guhwang, chrysanthemum, hairy, chymo (herb), shrunk and haegeum extracts were treated to 1 μg / ml per well, incubated for 24 hours, and then total cell lysate was 25 μL 1 당 reporter per well. It was obtained by lysis in lysis buffer (Promega, Madison, Wis.). A sample untreated group was used as a control. Reporter activity was measured using a microplate luminometer (BMG Labtech, Offenburg, Germany) with 25 μL of luciferin (USB, Cleveland, OH) per well.

As a result, bark (leaf, stem), cypress (bark), cypress (stem), 줄 (bark), castor (stem), castor (outpost), persimmon (stem), dried yellow, lump, zyme, jimo (Sec), shrunk and haegeum extracts showed a relative increase in reporter activity by more than 50% (experimental sample fluorescence value / untreated fluorescence value × 100 = increase rate (%)) (Table 3 and Figure 4).

Experimental Example 5 Analysis of Isoquacitrine Content in Plant Extracts

The above 13 plant extracts, which showed high Wnt / β-catenin signaling promoting ability, were 0.186ug / ml, 0.744ug / ml, 1.86ug / ml, 3.72 with quantitative isoquacitrin standard (Sigma, 11793), respectively. ug / ml and 9.30 ug / ml methanol solutions were prepared and tested under the following HPLC conditions to obtain a calibration curve and to determine the content of isoquacitrin from the calibration curve.

Considering the fact that many unknown components are detected due to the nature of plant extracts, this test extracts the UV spectrum of the peak from 3D stereoscopic data with the UV spectrum of the isoquacitrin standard. Verification was made by comparison.

HPLC conditions

Detector: PDA Detector (λ 260nm)

Column: CAPCELL PAK, UG-120, 4.6 * 250mm, 5um

Mobile phase A: 0.1% H ₃ PO ₄ in H ₂ O / Acetonitrile (85:15)

B: 0.1% H ₃ PO ₄ in Acetonitrile

        Gradient: 0min 0% B → 35min 70% B, maintain 10min

Flow rate: 0.8ml / min

As a result, the isoquacitrine content of castor (outpost) was the highest (1.01%), and the isoquacitrine content of the lumped (medicinal) was 0.11%.

Experimental Example 6 Skin Safety Experiment through Primary Stimulation Patch of Human Skin

Skin irritation evaluation of high concentrations of several extracts was carried out through a closed patch-test. The subjects of this study were 20 healthy adults in their 20s to 40s.

Samples were prepared by adding extracts (samples) prepared in the <Preparation of Plant Extracts> to the cosmetic base of Table 1 according to Table 4 below. At this time, 1.0 parts by weight of the sample of Table 5 was added to 100 parts by weight of purified water of Table 1, respectively. Meanwhile, the saline solution used as a control contained 0.9% of NaCl.

Take 25 μL of each sample, place it in an IQ chamber, attach it to the back, remove it after 24 hours, and after 30 minutes and 48 hours, quantify the side effects, itching and erythema, edema, etc. It calculated based on <Equation 1>. The judges were conducted by a professional researcher who was more than 10 years of the experiment.

<Formula 1>

Average stimulus judgment range = {Σ (evaluation standard number X number of people of corresponding evaluation value) / (total number of subjects X highest evaluation standard value)} × 100

Evaluation Criteria

+++ 2

++ 1.5

+ 1.0

+-0.5

- 0

Skin irritation judgment range

1 Minimum stimulation (10 or less)

2 mild irritation (greater than 10-less than -20)

3 Moderate stimulation (greater than 20-less than -30)

4 severe irritation (over 30)

As a result, they showed similar stimuli at 1 parts by weight, and showed a primary skin irritation response, which was not a significant result with saline, indicating that it was a safe substance for external application of skin (Table 4).

Experimental Example 7 Cellulite Improvement and Body Slimming Effect

Forty women in their twenties and thirties with a BMI (Body Mass Index) of 23 to 25 who had no other metabolic abnormalities detected (20 in each group) were applied to the thigh area twice daily, morning and evening, for eight weeks. After using the samples of Examples 1 to 7 prepared in <Preparation of Examples 1 to 7> before and after use for 8 weeks, by measuring the subcutaneous fat thickness after use to determine the slimming effect. At this time, the control group was set to the group using only the cosmetic base of Table 1, subjects were administered to maintain a regular life and a regular diet during the test period. As a measuring method, a reduced length (ΔL = L ₁₂ -L ₀ ) was measured using a ruler, and the result was expressed using a student's t-test.

As a result, the slimming effect of Example 1, Example 2, Example 3, and Example 7 was favorable, and the effect of Example 7 was especially favorable. In contrast, no significant changes in thigh circumference were observed in other experimental and control groups (Table 5).

Meanwhile, in Examples 1 to 7 and the control treatment, the thickness of the subcutaneous fat layer (in mm) was measured using Dermascan-C using ultrasound to determine the effect on cellulite, and the obtained value was student's t-test. Statistical significance was analyzed by comparing before and after use.

As a result, cellulite was most effectively alleviated when using Examples 1 and 2, and Example 7 also showed a significant effect.

Claims (10)

Cosmetic composition comprising castor extract as an active ingredient, cellulite prevention and improvement effect.
The method of claim 1,
Cosmetic composition, characterized in that it further comprises one or more selected from the group consisting of lump extract, chopsticks extract, cypress extract, 칡 extract, glycolysis extract, dried Guangjihwang extract, jimo extract, uniaxial extract and haegeum extract.
The method of claim 1,
Cosmetic composition, characterized in that it further comprises at least three selected from the group consisting of lump extract, bark tree extract, cypress extract, 칡 extract, glycolysis extract, geonjihwang extract, jimo extract, uniaxial extract and haegeum extract.
The method of claim 1,
The castor extract is a cosmetic composition, characterized in that it comprises isoquacitrin.
The method of claim 1,
A cosmetic composition, which is a cosmetic composition for skin slimming to be applied topically.
4. The method according to any one of claims 1 to 3,
The extract is a cosmetic composition, characterized in that extracted with hot water extraction or lower alcohol of 3 or less carbon atoms.
The method of claim 1,
The cosmetic composition is a cosmetic composition, characterized in that the skin absorption of the active ingredient is 0.01 μg / cm ² to 500 μg / cm ² .
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