KR101348109B1 - Apparatus for extracorporeal blood circulation, and vascular diseases diagnostic method - Google Patents

Abstract

The extracorporeal blood circulation device includes a first tube connected to an artery of a living body, a second tube connected to a vein of the living body, a blood storage container connected to each of the first tube and the second tube, and a blood storage container and the second tube. A third tube connecting between the portions, and a measuring unit for measuring at least one of an average flow rate and velocity distribution of the blood flow of the living organism through the third tube.

Description

Extracorporeal blood circulation, and cardiovascular disease research methods {APPARATUS FOR EXTRACORPOREAL BLOOD CIRCULATION, AND VASCULAR DISEASES DIAGNOSTIC METHOD}

The present invention relates to an extracorporeal blood circulation apparatus and a cardiovascular disease research method, and more particularly, to an extracorporeal blood circulation apparatus for measuring hemodynamical properties of living organisms, and a cardiovascular disease research method.

As the number of deaths from cardiovascular diseases in advanced countries has increased significantly, studies related to this have been actively conducted, and the link between these cardiovascular diseases and blood flow has been revealed. Accordingly, in order to obtain indicators for diagnosing circulatory diseases occurring in the cardiovascular system, various studies have been conducted to interpret blood flow phenomena associated with these diseases.

Conventional studies have flowed a physiological saline mixture infused with water or red blood cells to a circulation device, and changed the flow conditions of the mixture using a circulation pump. Under these conditions, a study of measuring the flow rate change of blood flow using a mixed solution and inferring the shear stress from it has been conducted. However, unlike the mixed solution, the actual blood changes in viscosity as the flow rate changes, and thus, the velocity distribution is different, so that the flow described by water or the mixed solution and the actual blood flow show a great difference.

In addition, other conventional studies have shown a method of collecting blood from living organisms, treating the blood to prevent blood coagulation, and then flowing blood into a transparent conduit to study blood flow. In this method, the flow conditions of the blood can be easily changed, but the change in the hemoheological properties of the blood becomes a problem. In detail, even when the anticoagulation treatment is performed to prevent blood coagulation, the shape of red blood cells changes as time passes, thereby affecting red blood cell aggregation, thereby changing rheological properties such as the viscosity of blood. Therefore, the indicator obtained from the outside of living organisms using whole blood should be verified by analyzing the blood flow in the body. However, the blood flow inside the blood vessels in the body is opaque and can only be measured using clinical imaging devices such as ultrasound or MRI phase imaging. Until now, blood flow information that could accurately calculate wall shear stress in blood vessels has not been obtained. On the other hand, other conventional studies are relatively easy to measure the blood flowing through the blood vessels around the skin, but it is difficult to change the flow conditions such as blood flow rate and pulsatility index (hemodynamical) There is a limit to research on properties and indicators.

One embodiment of the present invention is to solve the above-mentioned problems, to provide an extracorporeal blood circulation device, and a cardiovascular disease research method that can easily perform the study of hemodynamic properties and indicators associated with cardiovascular disease.

A first aspect of the present invention for achieving the above technical problem is a first tube connected to the arteries of life, a second tube connected to the vein of the life, a blood storage container connected to each of the first tube and the second tube, An in vitro comprising a third tube connecting between the blood reservoir and a portion of the second tube, and a measurement unit for measuring at least one of an average flow rate and velocity distribution of the blood flow of the organism passing through the third tube Provide a blood circulation device.

A first valve mounted to the first tube, a second valve mounted to the second tube and positioned between the blood reservoir and the portion of the second tube, and mounted to the second tube, And a third valve positioned between the portion of the two tubes and the vein of the living being.

The measuring unit may include one selected from an optical microscope, an image acquisition camera connected to the optical microscope, an imaging device using ultrasound, and an imaging device using X-rays.

The measurement unit may include the optical microscope and the image acquisition camera, and the third tube may be made of transparent ethylene propylene (FEP).

The measuring unit may include one selected from the imaging apparatus using the ultrasonic wave and the imaging apparatus using the X-ray, and the third tube may be made of an opaque material.

The shape of the third tube may have the same shape as stenosis, aneurysm, or bifurcation.

Each of the first tube and the second tube may include heparin filled therein.

In addition, the second aspect of the present invention provides a step of providing the extracorporeal blood circulation device, and passing through the third tube using each of the first valve, the second valve and the third valve of the extracorporeal blood circulation device. Pulsatility index is controlled by controlling at least one of the average flow rate and velocity distribution of the blood flow of the living being, and measuring the average flow rate of the living blood flow through the third tube using the measuring unit of the extracorporeal blood circulation device. It provides a cardiovascular disease research method comprising the step of calculating).

In addition, a third aspect of the present invention provides a measuring unit for measuring at least one of a fourth tube connected between an artery of a living organism and a vein of the living creature, and an average flow rate and velocity distribution of blood flow of the living organism passing through the fourth tube. It provides an extracorporeal blood circulation device comprising a.

It may further include a fourth valve mounted to the fourth tube.

The apparatus may further include a fifth valve mounted at one end of the fourth tube and a sixth valve mounted at the other end of the fourth tube.

According to one embodiment of the above-described problem solving means of the present invention, there is provided an extracorporeal blood circulation device, and a cardiovascular disease research method that can easily study the hemodynamic properties and indicators associated with cardiovascular disease.

1 is a view showing an extracorporeal blood circulation device according to a first embodiment of the present invention.
FIG. 2 is a view illustrating various shapes of the third tube illustrated in FIG. 1.
3 is a view showing an example of the measuring unit shown in FIG.
4 is a view showing another example of the measuring unit shown in FIG. 1.
5 to 8 are graphs for explaining the cardiovascular disease research method according to a second embodiment of the present invention.
9 is a view showing an extracorporeal blood circulation device according to a third embodiment of the present invention.
10 is a view showing an extracorporeal blood circulation device according to a fourth embodiment of the present invention.
11 is a view showing an extracorporeal blood circulation device according to a fifth embodiment of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. The present invention may be embodied in many different forms and is not limited to the embodiments described herein.

In order to clearly illustrate the present invention, parts not related to the description are omitted, and the same or similar components are denoted by the same reference numerals throughout the specification.

In addition, since the sizes and thicknesses of the respective components shown in the drawings are arbitrarily shown for convenience of explanation, the present invention is not necessarily limited to those shown in the drawings.

Also, throughout the specification, when an element is referred to as "including" an element, it is understood that the element may include other elements as well, without departing from the other elements unless specifically stated otherwise.

Hereinafter, the extracorporeal blood circulation apparatus according to the first embodiment of the present invention will be described with reference to FIGS. 1 to 4.

1 is a view showing an extracorporeal blood circulation device according to a first embodiment of the present invention.

As shown in FIG. 1, the extracorporeal blood circulation apparatus according to the first embodiment of the present invention is a device which is located outside the living body 10 such as a mouse, a rabbit, or a pig and is connected to the living body 10. 1 tube 100, second tube 200, blood storage container 300, third tube 400, measuring unit 500, first valve 600, second valve 700, third valve 800 and protective tube 900.

The first tube 100 is connected between the artery 11 of the living body 10 and the blood storage container 300. The first tube 100 has an inner diameter of 580um substantially, and is made of resin such as polyethylene (PE) or the like. The first tube 100 includes a heparin filled therein, thereby preventing blood clotting that may occur at the connection portion of the first tube 100 connected to the artery 11 of the living organism 10.

The second tube 200 is connected between the vein 12 of the living organism 10 and the blood storage container 300. The second tube 200 has an inner diameter of 580 um substantially and is made of resin such as polyethylene (PE) or the like. The second tube 200 includes a heparin filled therein, thereby preventing blood clotting that may occur at the connection portion of the second tube 200 connected to the vein 12 of the creature 10.

The blood storage container 300 is connected to each of the first tube 100, the second tube 200, and the third tube 400. The blood storage container 300 has a blood storage capacity of a predetermined amount (for example, 100ul) substantially, and may be made of a resin. The blood storage container is intended to suppress the formation of blood clots and may constitute a circulator without it.

The third tube 400 is connected between the blood storage container 300 and a portion 210 of the second tube 200. The third tube 400 has a narrower inner diameter than each of the first tube 100 and the second tube 200, and has an inner diameter of 100 μm. The third tube 400 may be made of transparent ethylene propylene (FEP).

FIG. 2 is a view illustrating various shapes of the third tube illustrated in FIG. 1. FIG. 2A shows a stenosis shape, FIG. 2B shows varicose and aneurysm shapes, and FIG. 2C shows a bifurcation shape.

As shown in (a) to (c) of FIG. 2, the shape of the third tube 400 may have the same shape as stenosis, varices and aneurysms, or bifurcation. For example, the third tube 400 may be manufactured to be the same as a site where a lot of cardiovascular disease occurs to analyze hemodynamic characteristics related to the cardiovascular disease. As an example of manufacturing the third tube 400, a gel is poured around a tube having the same shape as in FIGS. 2A to 2C, and after the gel is hardened, the tube is removed to remove the third tube ( 400) or PDMS channels, which are often used for microchannels, to form microstructures by patterning desired shapes on silicon wafers and pouring PDMS on them. Can be. Referring again to FIG. 1, the measurement unit 500 measures an average flow rate of blood flow, which is the flow of blood of the living organism 10 passing through the third tube 400. The measurement unit 500 includes a seating plate 510, a water drop 520, a microscope 530, and an image acquisition camera 540.

The seating plate 510 may be formed of a glass on which one region 410 of the third tube 400 is seated.

The water drop 520 is positioned on the seating plate 510 and accommodates a region 410 of the third tube 400. Since the third tube 400 accommodated in the water drop 520 is made of transparent FPI similar to the refractive index of water, the distortion of the tubular shape of the circular cross section of the third tube 400 is suppressed.

The microscope 530 corresponds to an area 410 of the third tube 400, and the image acquisition camera 540 is connected to the microscope 530 to measure blood flow of blood passing through the third tube 400. Shoot. The average flow velocity of the blood flow passing through the third tube 400 may be measured by observing the blood flow through the third tube 400 using the image acquisition camera 540. In detail, when a particle image velocimetry (PIV) algorithm is applied to an image captured by the image capturing camera 540, one or more of an average flow rate and a velocity distribution of blood flow may be measured.

3 is a view showing an example of the measuring unit shown in FIG. 4 is a view showing another example of the measuring unit shown in FIG. 1.

Meanwhile, as shown in FIG. 3, the measuring unit 500 may include an imaging device using X-rays or an imaging device using ultrasound as shown in FIG. 4. In this case, the size of the third tube 400 is not limited, and the material may be opaque. In detail, as shown in FIG. 3, an X-ray CCD camera is required to obtain an image through X-ray, and an average of blood flow is obtained by applying a particle image velocimetry (PIV) algorithm to the obtained image. One or more of the flow rate and velocity distribution can be measured.

In detail, as shown in FIG. 4, by applying a particle image velocimetry (PIV) algorithm using an ultrasonic B-mode image acquired by an ultrasonic transducer, an average flow velocity or velocity distribution of blood flow can be measured. have. Measuring ordinary tubes with ultrasound causes errors due to reflections in the tubes. So, ultrasonic measurement can reduce errors by making agarose phantom with similar impedance to water and making measurements in this area. Its fabrication involves drilling a hole in an acrylic box and fitting a tube with the desired shape. Then mix the agarose powder with water. Then heat to dissolve agarose powder, make agarose gel, pour it into acrylic box and cool until it hardens. After the gel has solidified, the tube is removed to produce an agarose phantom with channels. An ultrasonic sound absorber is placed on the bottom to remove the ultrasonic signal reflected from the bottom of the acrylic box. For ultrasonic measurements, the top of the agarose phantom must be filled with water.

Referring back to FIG. 1, the first valve 600 is mounted to the first tube 100, and adjusts the size of the inner diameter of the first tube 100 to allow blood flow to pass through the third tube 400. Control the average flow rate. For example, when the inner diameter of the first tube 100 is narrowed by using the first valve 600, the average flow velocity of blood flow through the third tube 400 decreases, and the pulsatility index also decreases. Here, the pulsation index is an index indicating a change in the waveform of the average flow velocity of blood flow.

The second valve 700 is mounted to the second tube 200 and is located between the blood storage container 300 and a portion 210 of the second tube 200. The second valve 700 controls the average flow velocity of the blood flow through the third tube 400 by adjusting the size of the inner diameter of the second tube 200 corresponding to the position where the second valve 700 is located. For example, when the inner diameter of the second tube 200 positioned between the blood storage container 300 and the portion 210 of the second tube 200 is narrowed by using the second valve 700, the third tube 400 is used. The mean flow velocity of blood flow through the s increases, and the pulsation index decreases as an exponential function.

The third valve 800 is mounted to the second tube 200 and is located between the portion 210 of the second tube 200 and the vein 12 of the living being 10. The third valve 800 controls the average flow rate of the blood flow through the third tube 400 by adjusting the size of the inner diameter of the second tube 200 corresponding to the position where the third valve 800 is located. For example, when the inner diameter of the second tube 200 located between the portion 210 of the second tube 200 and the vein 12 of the living organism 10 is narrowed by using the third valve 800, The average flow rate of blood flow through the tube 400 decreases and the pulsation index increases.

The protective tube 900 is disposed between the first valve 600 and the first tube 100 and between each of the second valve 700 and the third valve 800 and the second tube 200, and the first valve. It is suppressed that damage occurs to each of the first tube 100 and the second tube 200 by the operation of the 600, the second valve 700, and the third valve 800. The protective tube 900 has an inner diameter of 800um substantially, and is made of a resin such as silicone.

As described above, the extracorporeal blood circulation apparatus according to the first embodiment of the present invention is a novel concept of extracorporeal blood circulation combining the advantages of the internal blood measurement and the external circulation device for use in the study of the flow characteristics of blood flow associated with cardiovascular diseases. It is a device, because blood is not denatured because the actual blood circulating inside the living body 10 is used as it is, and since the blood flow is measured in an extracorporeal circulation loop that can arbitrarily change the shape of the blood vessel, the blood flow compared to the body Easy to measure

In addition, the extracorporeal blood circulation apparatus according to the first embodiment of the present invention uses the first valve 600, the second valve 700, and the third valve 800, respectively, to pass blood flow through the third tube 400. It is easy to change the mean flow rate and pulsation index of the blood vessel, making it easy to measure various hemodynamic features of blood flow and hemoheological changes such as blood velocity distribution, pulsation index, and erythrocyte aggregation. have. In other words, using the extracorporeal blood circulation device can study the various indicators of blood.

Hereinafter, a cardiovascular disease research method according to a second exemplary embodiment of the present invention will be described with reference to FIGS. 1 and 5 to 8.

First, the extracorporeal blood circulation apparatus according to the first embodiment of the present invention shown in FIG. 1 is provided.

Specifically, the extracorporeal blood circulation apparatus according to the first embodiment of the present invention described above is connected to the living body 10. The second tube 200 and the first tube 100 are connected to each of the vein 12 such as the jugular vein of the living body 10 such as a rat and the artery 11 such as the femoral artery to connect blood of the living body 10 to each other. The first tube 100, the second tube 200, and the third tube 400 are flowed to prevent degeneration of blood and maintain pulsation caused by the heart of the living body 10.

Next, using the first valve 600, the second valve 700 and the third valve 800 of the extracorporeal blood circulation device to control the average flow rate of the blood flow of the living organism 10 passing through the third tube 400 In addition, a pulsatility index is calculated by measuring at least one of an average flow rate and a velocity distribution of blood flow of the living organism 10 passing through the third tube 400 using the measurement unit 500 of the extracorporeal blood circulation device. .

Specifically, as shown in (a) of FIG. 5, particle image velocimetry (PIV) algorithms are applied to two consecutive images measured using the measuring unit 500, and FIG. Obtain the same velocity field information. At this time, if the cross section of the flow of blood flow does not change, since the velocity distribution is constant along the flow direction, the average is given along the flow direction to obtain more accurate velocity distribution information of the blood flow. Here, the image of FIG. 5 (a) was obtained by using a high speed camera using a 60 times lens, the Y axis of FIG. 5 (b) is the inner diameter direction of the third tube 400 and the X axis is the flow direction of blood flow. Indicates.

Using the velocity field information, a velocity distribution as shown in FIG. 6 (a) can be obtained, and the velocity field is measured by applying a particle image flowmeter algorithm to the entire image acquired for a predetermined time. Find the velocity distribution. If the maximum velocity value, which is the central value of the velocity distribution, is extracted over time, a change in the central velocity of blood flow flowing through the center of the third tube 400 as shown in FIG. 6 (b) may be obtained. Can be calculated. Here, the X axis of (a) of Figure 6 is the inner diameter direction of the third tube 400, the Y axis represents the speed of blood flow, the X axis of Figure 6 (b) is time and the Y axis is the center of the third tube 400 It represents the speed of blood flow.

FIG. 7 illustrates an average of blood flow through the third tube 400 when the inner diameter of the second tube 200 is reduced by using the second valve 700 in the extracorporeal blood circulation apparatus according to the first embodiment described above. It is a graph showing the change in flow rate and pulsation index accordingly. FIG. 8 illustrates the first tube 100 using the first valve 600 while reducing the inner diameter of the second tube 200 using the second valve 700 in the extracorporeal blood circulation apparatus according to the first embodiment described above. ), Or when the inner diameter of the second tube 200 is reduced by using the third valve 800, the mean flow velocity of blood flow through the third tube 400 and the change in pulsation index accordingly are shown. It is a graph.

In detail, as shown in FIG. 7, when the inner diameter of the second tube 200 is narrowed by using the second valve 700, the average flow velocity of the blood flow passing through the third tube 400 is increased, thereby pulsating. The exponent decreases as an exponential function.

In addition, by using the first valve 600 and the third valve 800, the inner diameter of each of the first tube 100 and the second tube 200 is narrowed, and the average flow velocity and pulsation of the blood flow passing through the third tube 400 are reduced. Control the exponent. As shown in FIG. 8, when the inner diameter of the first tube 100 is narrowed using the first valve 600, the average flow velocity and pulsation index of the blood flow passing through the third tube 400 decrease, and the third valve When the inner diameter of the second tube 200 is narrowed using the 800, the average flow velocity of blood flow through the third tube 400 decreases, but the pulsation index increases.

As such, each of the first valve 600, the second valve 700, and the third valve 800 may be used to control the average flow rate and pulsation index of the blood flow through the third tube 400. By using the blood circulation device, hemodynamic properties can be easily measured while maintaining the hemodynamic properties of the blood in the living body, and hemodynamic studies on various indicators can be performed by easily adjusting the flow conditions.

That is, the cardiovascular disease research method according to the second embodiment of the present invention by appropriately adjusting each of the first valve 600, the second valve 700, and the third valve 800 included in the extracorporeal blood circulation device, Since the average flow velocity and pulsation index of the blood flow passing through the third tube 400 can be easily changed, the influence of these flow condition changes on the erythrocyte aggregation or the velocity distribution change can be studied.

In addition, the cardiovascular disease research method according to the second embodiment of the present invention uses the first valve 600, the second valve 700, and the third valve 800, and at the same time, the third tube 400 which is a bypass conduit. Hemorrhagic characteristics related to these diseases can be analyzed in detail by forming the shape of the site of the cardiovascular disease such as stenosis, aneurysm, varicose, bifurcation.

In addition, the cardiovascular disease research method according to the second embodiment of the present invention can be applied to the pathology of related diseases when applied to an animal model having a circulatory disease or a blood rheological disease.

In addition, in the cardiovascular disease research method according to the second embodiment of the present invention, blood flow and red blood cell aggregation may be simultaneously observed using actual blood.

In addition, the cardiovascular disease research method according to the second embodiment of the present invention can be applied to plants or insects that can connect the first tube 100 and the second tube 200 in addition to mammals such as rats, rabbits and pigs. .

Hereinafter, the extracorporeal blood circulation apparatus according to each of the third to fifth embodiments of the present invention will be described with reference to FIGS. 9 to 11.

Hereinafter, only the characteristic portions different from the first embodiment will be described by taking excerpts, and the portions where the description is omitted are according to the first embodiment. In the third, fourth, and fifth embodiments of the present invention, the same elements are described with the same reference numerals as the first embodiment of the present invention for the convenience of description.

9 is a view showing an extracorporeal blood circulation device according to a third embodiment of the present invention.

As shown in FIG. 9, the extracorporeal blood circulation apparatus according to the third embodiment of the present invention is a device which is located outside the living body 10 such as a mouse, a rabbit, or a pig and is connected to the living body 10. 4 tube 403 and measurement unit 500.

The fourth tube 403 connects the artery 11 and the vein 12 of the living organism 10. The fourth tube 403 may be made of transparent ethylene propylene (FEP) or may be made of an opaque material.

As described above, the extracorporeal blood circulation apparatus according to the third embodiment of the present invention is a novel concept of extracorporeal blood circulation combining the advantages of the internal blood measurement and the external circulation device for use in the study of flow characteristics of blood flow associated with cardiovascular diseases. It is a device, because blood is not denatured because the actual blood circulating inside the living body 10 is used as it is, and since the blood flow is measured in an extracorporeal circulation loop that can arbitrarily change the shape of the blood vessel, the blood flow compared to the body Easy to measure

10 is a view showing an extracorporeal blood circulation device according to a fourth embodiment of the present invention.

As shown in FIG. 10, the extracorporeal blood circulation apparatus according to the fourth embodiment of the present invention is a device which is located outside the living body 10 such as a mouse, a rabbit, or a pig and is connected to the living body 10. A four tube 403, a measuring unit 500 and a fourth valve 704.

The fourth tube 403 connects the artery 11 and the vein 12 of the living organism 10. The fourth tube 403 may be made of transparent ethylene propylene (FEP) or may be made of an opaque material.

The fourth valve 704 is mounted to the fourth tube 403 and serves to adjust the inner diameter of the fourth tube 403.

As described above, the extracorporeal blood circulation apparatus according to the fourth embodiment of the present invention can easily change the average flow rate and pulsation index of the blood flow through the fourth tube 403 by using the fourth valve 704, thereby allowing the blood vessel to Various hemodynamic features of blood flow and hemoheological changes such as velocity distribution of blood flow, pulsation index and erythrocyte aggregation can be easily measured. In other words, using the extracorporeal blood circulation device can study the various indicators of blood.

11 is a view showing an extracorporeal blood circulation device according to a fifth embodiment of the present invention.

As shown in FIG. 11, the extracorporeal blood circulation apparatus according to the fifth embodiment of the present invention is a device which is located outside the living body 10 such as a mouse, a rabbit, or a pig and is connected to the living body 10. A four tube 403, a measuring unit 500, a fifth valve 705 and a sixth valve 706.

The fourth tube 403 connects the artery 11 and the vein 12 of the living organism 10. The fourth tube 403 may be made of transparent ethylene propylene (FEP) or may be made of an opaque material.

The fifth valve 705 is mounted at one end of the fourth tube 403, and the sixth valve 706 is mounted at the other end of the fourth tube 403. Each of the fifth valve 705 and the sixth valve 706 serves to adjust the inner diameter of the fourth tube 403.

As described above, the extracorporeal blood circulation apparatus according to the fifth embodiment of the present invention uses the fifth valve 705 and the sixth valve 706, respectively, and the average flow velocity and pulsation of blood flow through the fourth tube 403. The exponential change makes it easy to measure various hemodynamic features of blood flow and hemoheological changes, such as blood velocity distribution, pulsation index, and erythrocyte aggregation. In other words, using the extracorporeal blood circulation device can study the various indicators of blood.

While the invention has been shown and described with reference to certain preferred embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made therein without departing from the spirit and scope of the following claims. Those who are engaged in the technology field will understand easily.

First tube 100, second tube 200, blood storage vessel 300, third tube 400, measurement unit 500

Claims (11)

A first tube connected to the artery of life;
A second tube connected to the vein of the living organism;
A blood storage container connected to each of the first tube and the second tube;
A third tube connecting between the blood storage container and a portion of the second tube; And
An extracorporeal blood circulation device comprising a measurement unit for measuring at least one of an average flow rate and velocity distribution of the blood flow of the living organism passing through the third tube. In claim 1,
A first valve mounted to the first tube;
A second valve mounted to the second tube and positioned between the blood reservoir and the portion of the second tube; And
And a third valve mounted to the second tube, the third valve being located between the portion of the second tube and the vein of the living being. 3. The method of claim 2,
The measuring unit is an extracorporeal blood circulation device including one selected from an optical microscope, an image acquisition camera connected to the optical microscope, an imaging device using ultrasound, an imaging device using X-rays. 4. The method of claim 3,
The measuring unit includes the optical microscope and the image acquisition camera,
The third tube is an extracorporeal blood circulation device consisting of fluorinated ethylene propylene (FEP). 4. The method of claim 3,
The measuring unit includes a selected one of the imaging device using the ultrasound and the imaging device using the X-ray,
The third tube is an extracorporeal blood circulation device made of an opaque material. 4. The method of claim 3,
An extracorporeal blood circulation device having the same shape as the stenosis, varicose and aneurysm, or bifurcation. 3. The method of claim 2,
Wherein said first tube and said second tube each comprise heparin filled therein. delete A fourth tube connecting between the artery of the living thing and the vein of the living thing; And
A measuring unit measuring at least one of an average flow rate and a velocity distribution of the blood flow of the living organism passing through the fourth tube
In vitro blood circulation device comprising a. The method of claim 9,
An extracorporeal blood circulation device further comprising a fourth valve mounted to the fourth tube. The method of claim 9,
A fifth valve mounted to one end of the fourth tube; And
A sixth valve mounted to the other end of the fourth tube
Extracorporeal blood circulation device further comprising.

Images