KR101331199B1 - Method for producing bibimbab using food materials having excellent antidiabetic activity - Google Patents
Method for producing bibimbab using food materials having excellent antidiabetic activity Download PDFInfo
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- KR101331199B1 KR101331199B1 KR1020120054426A KR20120054426A KR101331199B1 KR 101331199 B1 KR101331199 B1 KR 101331199B1 KR 1020120054426 A KR1020120054426 A KR 1020120054426A KR 20120054426 A KR20120054426 A KR 20120054426A KR 101331199 B1 KR101331199 B1 KR 101331199B1
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Abstract
Description
본 발명은 현미 메밀밥, 청국장 소스, 곤약, 도라지, 브로콜리, 시금치, 표고버섯, 고사리, 양파, 무채 및 두부를 혼합하여 제조하는 것을 특징으로 하는 항당뇨 활성이 우수한 비빔밥의 제조방법 및 상기 방법으로 제조된 항당뇨 활성이 우수한 비빔밥에 관한 것이다.The present invention is a method of producing bibimbap with excellent anti-diabetic activity, characterized in that it is prepared by mixing brown rice buckwheat rice, Cheonggukjang sauce, konjac, bellflower, broccoli, spinach, shiitake mushroom, fern, onion, radish and tofu It relates to a bibimbap produced excellent antidiabetic activity.
세계적으로 건강 추구현상이 확산되면서 육식 중심의 식단을 지양하고 채식 위주의 식단을 선호하는 등 건강식에 대한 관심이 고조되고 있다. 이러한 측면에서 채소를 풍부하게 사용하고 기름지지 않은 동양음식에 대한 관심이 높아지고 있으며, 특히 한국음식은 맛과 영양면에서 우수한 음식으로 평가받고 있다.As the pursuit of health is spreading around the world, interest in health foods is increasing, such as refraining from meat-based diets and preferring vegetarian diets. In this respect, there is a growing interest in oriental foods that use vegetables abundantly and are not greasy. Especially, Korean food is regarded as excellent food in terms of taste and nutrition.
비빔밥은 밥에 여러 가지 나물과 쇠고기 볶음, 육회, 튀각, 참기름을 넣어 비벼먹는 우리나라 일품요리 중 하나이며, 한국의 대표적인 음식이다. 비빔밥에는 여러 가지 채소가 첨가되므로 다양한 비타민, 무기질, 섬유질을 비롯한 기능성분이 많이 함유되어 있어 건강식을 대표하는 음식이라 해도 손색이 없다.Bibimbap is one of Korea's finest dishes, mixed with various herbs, beef stir-fry, sashimi, tempura, and sesame oil. Bibimbap is added to a variety of vegetables, so a variety of vitamins, minerals, fiber and other functional ingredients are a good way to represent a healthy food.
이러한 추세에 따라 전통적인 한국음식 중 비빔밥에 대한 관심이 증가하고 있으며, 비빔밥에 대한 기호도 조사를 비롯하여 영양성, 기능성, 위해성 평가, 비빔밥의 표준조리법 등 비빔밥에 대한 연구가 활발히 진행되고 있다. 그러나 대부분의 연구가 콩나물 비빔밥 및 전주비빔밥을 비롯한 기존의 비빔밥을 위주로 연구되고 있으며, 새로운 비빔밥 개발에 대한 시도는 많으나 이에 대한 연구 보고는 일부에 불과하다.In line with these trends, interest in bibimbap among traditional Korean foods is increasing, and research on bibimbap such as nutrition, functionality, risk assessment, and standard cooking methods of bibimbap, including bibimbap, is being actively conducted. However, most of the research is focused on existing bibimbap, including bean sprouts bibimbap and Jeonju bibimbap, and many attempts have been made to develop new bibimbap, but only a few reports have been made.
비빔밥과 관련된 특허는 첨가되는 비빔밥 재료에 따라 해산물을 이용한 비 빔밥(해초, 해삼, 멍게, 전복, 해물 등), 채소를 이용한 비빔밥(생야채, 메밀싹, 뽕잎, 약초, 금산채, 녹차, 새싹)과 모듬장아찌, 버섯불고기, 견과류 비빔밥에 관한 내용이 있으며, 그 외에도 비빔밥 소스 및 비빔밥 용기와 관련된 내용이 주를 이루고 있다.Patents related to bibimbap include bibimbap using seafood (seaweed, sea cucumber, sea urchin, abalone, seafood, etc.) and bibimbap using vegetables (raw vegetables, buckwheat sprouts, mulberry leaves, herbs, Geumsanchae, green tea, sprouts) And bibimbap, assorted pickles, mushrooms bulgogi and nuts, and bibimbap sauce and bibimbap containers.
한국등록특허 제0522411호에는 생야채 비빔밥이 개시되어 있으나, 본 발명의 항당뇨 활성이 우수한 식재를 이용한 비빔밥의 제조방법과는 상이하다.Korean Patent No. 0522411 discloses a raw vegetable bibimbap, but it is different from the method for producing bibimbap using a plant material having excellent antidiabetic activity.
본 발명은 상기와 같은 요구에 의해 도출된 것으로서, 본 발명에서는 항당뇨 활성이 높은 비빔밥을 개발하기 위해, 항당뇨 활성이 높은 식재료 중 비빔밥 제조 시 적합하고 영양, 맛, 모양 및 식감 등의 재료들 간의 궁합을 고려하여 선정된 재료들인 청국장 소스, 곤약, 도라지, 브로콜리, 시금치, 표고버섯, 고사리, 양파, 무채 및 두부를 현미 메밀밥에 적정량 첨가하여 제조함으로써, 현재 건강식으로 주목받고 있는 한국의 향토음식인 비빔밥의 소비를 증진시키고, 기존의 비빔밥에 비해 맛과 영양면에서도 우수하여 소비자들에게 신뢰받을 수 있는 항당뇨 활성이 증진된 비빔밥을 개발하는 데 그 목적이 있다.The present invention is derived from the above requirements, in the present invention, in order to develop bibimbap with high anti-diabetic activity, suitable ingredients for producing bibimbap among foods with high anti-diabetic activity, such as nutrition, taste, shape and texture In consideration of the compatibility between the ingredients, Cheonggukjang sauce, konjac, bellflower, broccoli, spinach, shiitake mushrooms, bracken, onion, radish and tofu are prepared by adding appropriate amounts to brown rice buckwheat rice, which is now gaining attention in Korea. The purpose of the present invention is to improve the consumption of bibimbap, which is a food, and to improve the anti-diabetic activity, which is excellent in taste and nutrition compared to the existing bibimbap, which can be trusted by consumers.
상기 과제를 해결하기 위해, 본 발명은 현미 메밀밥, 청국장 소스, 곤약, 도라지, 브로콜리, 시금치, 표고버섯, 고사리, 양파, 무채 및 두부를 혼합하여 제조하는 것을 특징으로 하는 항당뇨 활성이 우수한 비빔밥의 제조방법을 제공한다.In order to solve the above problems, the present invention is bibimbap with excellent anti-diabetic activity, characterized in that the mixture of brown rice buckwheat rice, Cheonggukjang sauce, konjac, bellflower, broccoli, spinach, shiitake mushrooms, bracken, onion, radish and tofu It provides a method of manufacturing.
또한, 본 발명은 상기 방법으로 제조된 항당뇨 활성이 우수한 비빔밥을 제공한다.The present invention also provides a bibimbap excellent in antidiabetic activity prepared by the above method.
본 발명의 항당뇨 활성이 우수한 식재료들을 이용하여 제조된 비빔밥은 기름지지 않으면서 맛과 식감에서 우수하여 소비자들의 기호도를 증진시킬 수 있을 뿐만 아니라, 기존의 시판 비빔밥에 비해 항당뇨 성분 등의 기능성 물질이 다량으로 함유되어 있어 기존의 시판 비빔밥과는 차별화된 기능성 비빔밥을 제공할 수 있으며, 향토 음식인 비빔밥 시장 확대에 기여할 것이라 판단된다.Bibimbap prepared by using the food ingredients excellent in anti-diabetic activity of the present invention is excellent in taste and texture without being greasy, thereby improving consumer preference, and functional substances such as anti-diabetic components compared to conventional commercial bibimbap. Since it is contained in a large amount, it can provide a functional bibimbap different from the existing commercial bibimbap, and it is expected to contribute to the expansion of the local bibimbap market.
도 1은 본 발명의 방법으로 완성된 비빔밥을 보여주는 사진이다.
도 2는 본 발명의 비빔밥을 동결건조 및 분쇄한 후의 사진을 보여준다.1 is a photograph showing a bibimbap completed by the method of the present invention.
Figure 2 shows a photograph after lyophilization and grinding the bibimbap of the present invention.
본 발명의 목적을 달성하기 위하여, 본 발명은 현미 메밀밥, 청국장 소스, 곤약, 도라지, 브로콜리, 시금치, 표고버섯, 고사리, 양파, 무채 및 두부를 혼합하여 제조하는 것을 특징으로 하는 항당뇨 활성이 우수한 비빔밥의 제조방법을 제공한다.In order to achieve the object of the present invention, the present invention is an anti-diabetic activity characterized in that it is prepared by mixing brown rice buckwheat rice, Cheonggukjang sauce, konjac, bellflower, broccoli, spinach, shiitake mushroom, fern, onion, radish and tofu It provides an excellent method for producing bibimbap.
본 발명의 비빔밥의 제조방법에서, 상기 청국장 소스는 멸치육수에 청국장, 천일염 및 다진마늘을 혼합한 후 끓여 제조할 수 있으나, 이에 제한되지 않는다. 본 발명의 비빔밥에 혼합되는 청국장 소스는 일반 고추장이 아닌 항당뇨 활성이 우수한 식재료인 청국장을 비빔밥 소스에 적합하도록 제조하여, 비빔밥의 풍미를 증진시킬 뿐만 아니라 항당뇨 등의 기능성을 증진시킬 수 있었다.In the method of producing bibimbap of the present invention, the Cheonggukjang sauce may be prepared by mixing the anchovy broth with Cheonggukjang, sun-dried salt and chopped garlic, and then boiling it, but not limited thereto. Cheonggukjang sauce to be mixed in the bibimbap of the present invention was prepared to be suitable for the bibimbap source of the cheonggukjang, a food material with excellent anti-diabetic activity other than the general gochujang, not only to enhance the flavor of bibimbap but also to improve the functionality of anti-diabetic.
본 발명의 비빔밥은 바람직하게는 현미 메밀밥 160~200 중량부, 청국장 소스 30~50 중량부, 곤약 16~24 중량부, 도라지 16~24 중량부, 브로콜리 16~24 중량부, 시금치 16~24 중량부, 표고버섯 16~24 중량부, 고사리 16~24 중량부, 양파 16~24 중량부, 무채 16~24 중량부 및 두부 16~24 중량부를 혼합하여 제조할 수 있으며, 더욱 바람직하게는 현미 메밀밥 180 중량부, 청국장 소스 40 중량부, 곤약 20 중량부, 도라지 20 중량부, 브로콜리 20 중량부, 시금치 20 중량부, 표고버섯 20 중량부, 고사리 20 중량부, 양파 20 중량부, 무채 20 중량부 및 두부 20 중량부를 혼합하여 제조할 수 있다. 본 발명의 비빔밥의 상기 재료들은 항당뇨 활성이 높은 식재료들 중 비빔밥 제조 시 적합하고 영양, 맛, 모양 및 식감 등의 재료들 간의 궁합이 잘 맞고, 또한, 비빔밥 프랜차이즈 사업을 위해 재료들의 수급 용이성과 관리의 간편화를 고려하여 재료들을 선정한 것이다. 밥은 일반 흰쌀이 아닌 현미에 섬유소가 많은 메밀을 사용하였고, 선정된 채소 및 과일은 혈당을 떨어뜨리는 데 도움을 주는 곤약 및 도라지와 브로콜리, 시금치, 표고버섯, 고사리 및 양파 등을 사용하였으며, 또한, 단백질 보충을 위해 두부도 사용하였다.Bibimbap of the present invention is preferably 160 to 200 parts by weight brown rice buckwheat rice, 30 to 50 parts by weight of Cheonggukjang sauce, 16 to 24 parts by weight of konjac, 16 to 24 parts by weight of bellflower, 16 to 24 parts by weight of broccoli, 16 to 24 spinach It can be prepared by mixing parts by weight, 16 to 24 parts by weight of shiitake mushrooms, 16 to 24 parts by weight of fern, 16 to 24 parts by weight of onion, 16 to 24 parts by weight of radish, and 16 to 24 parts by weight of tofu, and more preferably brown rice Buckwheat rice 180 parts, Cheonggukjang sauce 40 parts, Konnyaku 20 parts, Bellflower 20 parts, Broccoli 20 parts, Spinach 20 parts, Shiitake mushrooms 20 parts, Fern 20 parts, Onions 20 parts, Radish 20 It can be prepared by mixing 20 parts by weight of the weight part and tofu. The above-mentioned ingredients of bibimbap of the present invention are suitable for the production of bibimbap among the foods having high anti-diabetic activity, and are well-matched among ingredients such as nutrition, taste, shape, and texture, and also, the bibimbab franchise business is easy The materials were selected for ease of management. Rice is a high-fiber buckwheat in brown rice, not ordinary white rice, and selected vegetables and fruits use konjac and bellflower, broccoli, spinach, shiitake mushrooms, ferns, and onions to help lower blood sugar. Tofu was also used for protein supplementation.
본 발명의 비빔밥의 제조방법은 구체적으로는Bibimbap production method of the present invention is specifically
(a) 현미와 메밀을 8~10:0.5~1.5의 중량비율로 혼합한 후 씻어 현미 메밀밥을 짓는 단계;(a) mixing brown rice and buckwheat at a weight ratio of 8 to 10: 0.5 to 1.5 and washing to make brown rice buckwheat rice;
(b) 멸치육수에 청국장, 천일염 및 다진마늘을 혼합한 후 끓여 청국장 소스를 제조하는 단계;(b) mixing the anchovy broth with Cheonggukjang, sun-dried salt and chopped garlic and then boiling to produce Cheonggukjang sauce;
(c) 곤약, 브로콜리 및 두부를 각각 끓는 물에 데쳐 준비하는 단계;(c) preparing konjac, broccoli and tofu by boiling each in boiling water;
(d) 도라지, 표고버섯, 고사리 및 양파를 각각 볶아 준비하는 단계;(d) preparing roasted bellflower, shiitake mushrooms, ferns and onions, respectively;
(e) 무채와 데친 시금치 각각에 양념을 하여 준비하는 단계; 및(e) seasoning and preparing each of the radish and poached spinach; And
(f) 상기 (a)단계의 현미메밀밥 160~200 중량부에 상기 (b)단계의 제조한 청국장 소스 30~50 중량부, 상기 (c)단계의 준비한 곤약 16~24 중량부, 브로콜리 16~24 중량부 및 두부 16~24 중량부, 상기 (d)단계의 준비한 도라지 16~24 중량부, 표고버섯 16~24 중량부, 고사리 16~24 중량부 및 양파 16~24 중량부와 상기 (e)단계의 준비한 무채 16~24 중량부 및 시금치 16~24 중량부를 혼합하는 단계를 포함할 수 있으며,(f) 160 to 200 parts by weight of brown rice buckwheat in step (a) 30 to 50 parts by weight of the prepared soybean paste sauce in step (b), 16 to 24 parts by weight of konjac prepared in step (c), broccoli 16 ~ 24 parts by weight and 16 to 24 parts by weight of tofu, 16 to 24 parts by weight of the bellflower prepared in step (d), 16 to 24 parts by weight of shiitake mushrooms, 16 to 24 parts by weight of fern and 16 to 24 parts by weight of onion ( e) 16 to 24 parts by weight of the prepared radish prepared in step and may comprise the step of mixing 16 to 24 parts by weight of spinach,
더욱 구체적으로는More specifically,
(a) 현미와 메밀을 9:1의 중량비율로 혼합한 후 씻어 현미 메밀밥을 짓는 단계;(a) mixing brown rice and buckwheat in a weight ratio of 9: 1 and washing to make brown rice buckwheat rice;
(b) 멸치육수에 청국장, 천일염 및 다진마늘을 혼합한 후 끓여 청국장 소스를 제조하는 단계;(b) mixing the anchovy broth with Cheonggukjang, sun-dried salt and chopped garlic and then boiling to produce Cheonggukjang sauce;
(c) 곤약, 브로콜리 및 두부를 각각 끓는 물에 데쳐 준비하는 단계;(c) preparing konjac, broccoli and tofu by boiling each in boiling water;
(d) 도라지, 표고버섯, 고사리 및 양파를 각각 볶아 준비하는 단계;(d) preparing roasted bellflower, shiitake mushrooms, ferns and onions, respectively;
(e) 무채와 데친 시금치 각각에 양념을 하여 준비하는 단계; 및(e) seasoning and preparing each of the radish and poached spinach; And
(f) 상기 (a)단계의 현미메밀밥 180 중량부에 상기 (b)단계의 제조한 청국장 소스 40 중량부, 상기 (c)단계의 준비한 곤약 20 중량부, 브로콜리 20 중량부 및 두부 20 중량부, 상기 (d)단계의 준비한 도라지 20 중량부, 표고버섯 20 중량부, 고사리 20 중량부 및 양파 20 중량부와 상기 (e)단계의 준비한 무채 20 중량부 및 시금치 20 중량부를 혼합하는 단계를 포함할 수 있다.(f) 40 parts by weight of the prepared Cheonggukjang sauce of step (b), 20 parts by weight of prepared konnyaku of step (c), 20 parts by weight of broccoli and 20 parts of tofu To 20 parts by weight of the prepared bellflower of step (d), 20 parts by weight of shiitake mushrooms, 20 parts by weight of fern and onion 20 parts by weight of the prepared radish and 20 parts by weight of spinach prepared in step (e) It may include.
본 발명은 또한, 상기 방법으로 제조된 항당뇨 활성이 증진된 비빔밥을 제공한다.
The present invention also provides bibimbap enhanced antidiabetic activity prepared by the above method.
이하, 본 발명을 실시예에 의해 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다.
Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.
제조예Manufacturing example 1: One: 항당뇨Anti-diabetic 활성의 식재를 이용한 비빔밥 제조 Bibimbap Preparation Using Active Ingredients
(1) 비빔소스 제조(1) Bibimb Sauce Manufacturing
비빔소스는 멸치육수 50 ml에 생청국장 4큰술, 천일염 1작은술, 다진마늘 1작은술의 분량을 넣어 약한 불로 졸여 청국장 소스를 만들었다.
Bibimbose sauce was made with 50 ml of anchovy broth and 4 tablespoons of raw Cheonggukjang, 1 teaspoon sun-dried salt, and 1 teaspoon minced garlic.
(2) 비빔밥 재료 및 만드는 법(2) bibimbap ingredients and how to make
(a) 현미와 메밀은 9:1의 비율로 씻어 고슬고슬하게 밥을 지었다.(a) Brown rice and buckwheat were washed in a 9: 1 ratio and cooked thoroughly.
(b) 곤약은 4 cm의 길이로 곱게 썰어 끓는 물에 데쳐 준비하였다.(b) The konjac was cut into 4 cm lengths and boiled in boiling water.
(c) 도라지는 소금물에 넣어 여러 번 주물러 씻어 쓴맛을 충분하게 빼주었다. 쓴맛을 제거한 도라지는 4 cm의 길이로 썰어 준비한 후 마늘, 참기름의 양념을 하여 팬에 볶아내어 준비하였다.(c) Put the bellflower in salted water and rub it several times to get enough bitterness. After removing the bitter taste, the bellflower was prepared by cutting into 4 cm lengths, and seasoned with garlic and sesame oil.
(d) 브로콜리는 끓는 물에 소금을 넣고 살짝 데쳐 낸 후 찬물에 헹궈 준비하였다.(d) Broccoli was prepared by putting salt in boiling water, blanching and rinsing in cold water.
(e) 시금치는 다듬어 끓는 물에 소금을 넣고 파랗게 데쳐 낸 후 찬물에 헹궜다. 헹군 시금치의 물기를 꽉 짠 후 소금, 마늘, 참기름, 깨소금에 양념하여 4 cm 의 길이로 썰어 준비하였다.(e) Spinach was trimmed, salted in boiling water, blanched blue, and rinsed in cold water. After squeezing the water of rinsed spinach tight, seasoned with salt, garlic, sesame oil, sesame salt and cut into 4 cm length prepared.
(f) 표고버섯은 건 표고버섯으로 구입하여 하룻밤이상 미지근한 물에 불려 놓아 기둥을 잘라내었다. 불린 표고버섯은 가운데를 포 떠 최대한 얇게 채 썰어 준비하고 간장, 마늘, 참기름, 깨소금에 양념하여 기름에 살짝 볶아 준비하였다.(f) Shiitake mushrooms were purchased as dried shiitake mushrooms and soaked in lukewarm water overnight to cut off the columns. Soaked shiitake mushrooms were prepared by slicing them in the middle and slicing them in soy sauce, garlic, sesame oil, and sesame salt.
(g) 고사리는 마른 고사리를 구입하여 찬물에 하룻밤 이상 담가 놓아 통통하게 불으면 뜨거운 물에 데쳐낸 후 물에 담가 아린맛을 우려내었다. 식힌 고사리는 3 cm로 썰어 놓고 간장, 마늘, 참기름, 깨소금에 양념하여 기름에 살짝 볶아 준비하였다.(g) The fern purchased a dry fern, soaked in cold water for more than one night, and when it is plump, boil it in hot water and soak it in water to make it taste good. Cooled bracken was cut into 3 cm and seasoned with soy sauce, garlic, sesame oil, and sesame salt and lightly roasted.
(h) 양파는 채를 썰어 기름에 살짝 볶아 준비하였다.(h) Onions prepared by slicing vegetables and roasting slightly in oil.
(i) 무는 4 cm 정도로 채 썰어 놓고 소금, 고춧가루, 깨소금 혼합하여 빨갛게 양념하여 준비하였다.(i) Radish was cut into 4 cm and prepared by seasoning with red, salt, red pepper powder and sesame salt.
(j) 두부는 가로 1.5 cm, 세로 1.5 cm, 두께 0.8 cm로 네모지게 썰어 굵은 소금으로 살짝 뿌려 놓은 후 끓는 물에 데쳐 준비하였다.(j) Tofu was cut into 1.5 cm, 1.5 cm, and 0.8 cm thick cubes, sprinkled with coarse salt, and then boiled in boiling water.
(k) 고슬고슬하게 지어진 현미 메밀밥 180 g에 상기 제조한 청국장 소스 40 g과 상기 준비한 곤약 20 g, 도라지 20 g, 브로콜리 20 g, 시금치 20 g, 표고버섯 20 g, 고사리 20 g, 양파 20 g, 무채 20 g 및 두부 20 g을 먹기 좋게 담아 완성하였다(도 1).
(k) To 180 g of brown rice buckwheat rice, made with gritty rice, 40 g of the prepared soy sauce and 20 g of the prepared konnyaku, 20 g of bellflower, 20 g of broccoli, 20 g of spinach, 20 g of shiitake mushrooms, 20 g of onions, 20 onions g, 20 g of radish and 20 g of tofu was finished to eat well (Fig. 1).
1. 실험방법1. Experimental Method
(a) 동결건조 (a) Lyophilization
상기 제조예 1의 방법으로 제조된 비빔밥 1인분(400 g)을 동결 건조하여 100 mesh 이하로 분쇄하였다(도 2).
Bibimbap 1 serving (400 g) prepared by the method of Preparation Example 1 was lyophilized and pulverized to 100 mesh or less (FIG. 2).
(b) 일반성분 분석 (b) general component analysis
일반성분은 AOAC 방법에 준하여 분석하였다. 즉, 수분은 105℃ 상압가열건조법, 조회분은 550℃ 직접회화법, 조단백질은 kjeldahl 법, 조지방은 soxhlet 추출법으로 분석하였다.
General components were analyzed according to the AOAC method. In other words, moisture was analyzed at 105 ℃ atmospheric pressure drying, crude ash at 550 ℃ direct liquefaction, crude protein at kjeldahl, crude fat at soxhlet extraction.
(c) 비타민 C(c) vitamin C
비타민 C 분석은 식품공전에 명시되어 있는 인도페놀(indophenol) 적정법에 의해 정량하였다. 먼저, 시험용액의 조제를 위해 시료(비빔밥 동결건조 분말) 1 g을 칭량한 후 정확히 같은 양의 메타인산-초산용액을 잘 혼합해서 균등한 죽 상태로 한다. 죽상태의 일정량(W g)을 100 mL의 메스플라스크에 옮기고 묽은 메타인산-초산용액으로 100 mL로 하였다. 이 용액을 여과하여 시험용액으로 하였다. 상기 조제한 시험용액 10 mL를 삼각플라스크에 정확히 취하여 즉시 인도페놀용액으로 적어도 5초간 적색이 지속될 때까지 적정하였고, 이때 적정된 인도페놀용액의 소비량을 S mL로 하고 시료 중의 환원형 비타민 C 함량은 하기 식에 의해 구하였다.Vitamin C analysis was quantified by the indophenol titration method specified in the Food Code. First, 1 g of a sample (bibimbap lyophilized powder) is weighed to prepare a test solution, and then exactly the same amount of metaphosphate-acetic acid solution is mixed well to make an even porridge. A certain amount (W g) of porridge was transferred to a 100 mL volumetric flask, and diluted to 100 mL with dilute metaphosphate-acetic acid solution. This solution was filtered to give a test solution. 10 mL of the prepared test solution was precisely taken in an Erlenmeyer flask and immediately titrated with indophenol solution until the red color persisted for at least 5 seconds. At this time, the consumption amount of the indophenol solution was set to S mL and the reduced vitamin C content in the sample was as follows. It calculated | required by the formula.
환원형비타민 C(mg/100 g)= A mg×S/T×10×(시료채취량×2/W)×(100/시료채취량(g)Reduced Vitamin C (mg / 100 g) = A mg × S / T × 10 × (sample collection × 2 / W) × (100 / sample collection (g)
A mg: 인도페놀용액 T mL에 대응하는 아스코르빈산량
A mg: Ascorbic acid amount corresponding to T mL of indophenol solution
(d) 무기질(d) minerals
무기질은 식품공전의 미량영양성분시험법에 의해 분석하였다. 즉, 동결 건조된 비빔밥을 분쇄하여 분해 플라스크에 10 g씩 취하고 질산을 15 mL 가하여 서서히 약하게 가열하고 최초의 심한 반응이 끝난 후 다시 온도를 올려 가열시켰다. 질산이 휘산되어 내용물이 건고될 때까지 가열하고 질산과 증류수를 1:2로 혼합한 용액 10 mL와 과염소산 용액 30 mL를 가하여 서서히 가열시켰다. 고형물이 완전히 용해되고 액이 거의 무색이 될 때까지 가열을 계속하여 분해하고, 분해 후 증발접시에 액을 씻어 옮기고 과염소산을 증발시켰다. 잔류물에 염산과 증류수를 1:2로 혼합한 용액을 10 mL 가하여 수욕상에서 가온하여 완전히 녹인 후 100 mL로 정용하여 시험용액으로 하였다.Minerals were analyzed by the micronutrient test method of the Food Code. That is, lyophilized bibimbap was pulverized, and each 10 g was taken into a decomposition flask, 15 mL of nitric acid was added thereto, and the mixture was slowly heated slightly. After the initial severe reaction, the temperature was raised again. Heated until nitric acid was volatilized and the contents dried, and 10 mL of a mixture of nitric acid and distilled water 1: 2 and 30 mL of perchloric acid solution were slowly heated. The heating was continued to decompose until the solids were completely dissolved and the liquid became almost colorless. After the decomposition, the liquid was washed and transferred to the evaporation dish to evaporate perchloric acid. 10 mL of a solution mixed with hydrochloric acid and distilled water at 1: 2 was added to the residue, which was then warmed in a water bath to completely dissolve and then diluted to 100 mL to obtain a test solution.
인(P)을 제외한 나머지 원소들은 원자흡광분광광도계(Solaar M5, Thermo elemental Ltd., England)로 분석하였고, 인(P)은 몰리브덴 청(molybden blue) 비색법으로 분석하였다. 즉, 시험용액 1 mL에 몰리브덴산암모늄 2 mL, 하이드로퀴논(hydroquinone) 2 mL를 첨가한 후 혼합한 뒤 Na2SO3 2 mL를 넣고 증류수를 채워 총 25 mL로 만든 다음 30분 반응시켜서 분광광도계(UV-1650PC, Shimadzu Co., Kyoto, Japan)로 650 nm에서 측정하였다.
The elements other than phosphorus (P) were analyzed by atomic absorption spectrophotometer (Solaar M5, Thermo elemental Ltd., England), and phosphorus (P) was analyzed by molybdenum blue colorimetric method. That is, add 2 mL of ammonium molybdate and 2 mL of hydroquinone to 1 mL of the test solution, mix, add 2 mL of Na 2 SO 3 , fill it with distilled water, make a total of 25 mL, and react for 30 minutes. (UV-1650PC, Shimadzu Co., Kyoto, Japan) was measured at 650 nm.
(e) 유리 아미노산(e) free amino acids
비빔밥의 유리 아미노산 함량은 아미노산 자동분석기(S433, Sykam Co, Germany)를 이용하여 분석하였다. 시료를 1 g에 70% 에탄올을 가하여 마이크로파 시료전처리 장치(Mars X, CEM Co., USA)로 80℃에서 15분간 추출하였다. 감압여과 후 잔사에 다시 70% 에탄올을 가하여 위와 같은 조건으로 반복 추출하였다. 추출액을 45℃ 이하에서 감압 농축하여 물을 제거한 후 분액깔때기에 옮겨 에테르를 가하여 지방층을 제거하고 분액받은 물층을 45℃ 이하에서 감압농축하여 용매를 완전히 제거하였다. sample dillution buffer(pH 2.2)로 용해하여 10 mL로 정용하고 0.2 ㎛ syringe membrane filter(ChromTECH, Inc., MN, U.S.A)로 여과하였다. 이것을 amino acid analysis system(Sykam S-4300, Germany)을 이용하여 다음의 표 1과 같은 조건으로 분석하였다.The free amino acid content of bibimbap was analyzed using an amino acid automatic analyzer (S433, Sykam Co, Germany). 70% ethanol was added to 1 g of the sample, and the sample was extracted at 80 ° C. for 15 minutes with a microwave sample pretreatment device (Mars X, CEM Co., USA). After filtration under reduced pressure, 70% ethanol was added to the residue, and the mixture was repeatedly extracted under the same conditions. The extract was concentrated under reduced pressure at 45 ° C. to remove water, and then transferred to a separatory funnel to remove ether by adding ether, and the separated water layer was concentrated under reduced pressure at 45 ° C. to completely remove solvent. The solution was dissolved in sample dillution buffer (pH 2.2), purified to 10 mL, and filtered through a 0.2 μm syringe membrane filter (ChromTECH, Inc., MN, U.S.A). This was analyzed using the amino acid analysis system (Sykam S-4300, Germany) under the conditions shown in Table 1 below.
(f) 지방산(f) fatty acids
시료의 지방산 추출은 식품공전법에 준하여 추출하였다. 즉, 동결건조된 비빔밥을 분쇄하여 일정량의 에테르를 가하여 추출한 후 여과하였다. 여과액을 모두 합하여 분액 깔대기에 옮기고 소량의 증류수를 넣어 혼합한 후 에테르층을 분리하고 무수 Na2SO4으로 탈수시켜 여과하였다. 이어 여과액을 회전진공농축장치(rotary vacuum evaporator)로 40℃에서 감압 농축하여 용매를 제거한 후 총 지질을 얻었다. 지방산 조성은 지질을 메틸 에스테르화시킨 후 가스 크로마토그래피(6890N, Agilent technology, USA)로 분석하였다. Fatty acid extraction of the sample was carried out according to the Food Code. That is, lyophilized bibimbap was pulverized, and then extracted by adding a predetermined amount of ether and filtered. All the filtrates were combined, transferred to a separatory funnel, mixed with a small amount of distilled water, the ether layer was separated, dehydrated with anhydrous Na 2 SO 4 , and filtered. The filtrate was then concentrated under reduced pressure at 40 ° C. with a rotary vacuum evaporator to remove the solvent to obtain total lipids. Fatty acid composition was analyzed by gas chromatography (6890N, Agilent technology, USA) after methyl esterification of lipids.
즉, 추출한 지질 0.2 g에 0.5 N NaOH/methanol 5 mL를 넣고 10분간 수욕 상에서 가수분해시킨 후 14% BF3-methanol 5 mL를 가하여 2분간 가열하여 메틸 에스테르화시킨 다음 n-헥산으로 추출하여 지방산 분석용 시료로 사용하였다. 이때 GC의 분석조건은 컬럼은 SP-2560(100 m×0.25 mm, film thickness 0.20 μm)를 사용하였고, detector는 FID(flame ionization detector)를 사용하였다. 컬럼의 초기온도는 140℃이었고 5분간 유지한 다음 4℃/min로 240℃까지 온도를 상승시켜 19분간 유지하였다. 주입기(injector)와 검출기(detector)의 온도는 260℃로 하였고 캐리어 가스(carrier gas)는 N2를 사용하였으며 유속은 0.8 mL/min이었다. 각 지방산은 동일조건에서 표준지방산 methyl ester mixture(supelco 37 comp FAME Mix 10mg/ml in CH2Cl2)와 retention time을 비교하여 동정하였다.
That is, 0.2 g of the extracted lipid was added with 5 mL of 0.5 N NaOH / methanol, hydrolyzed in a water bath for 10 minutes, and 5 mL of 14% BF 3 -methanol was added thereto, heated for 2 minutes, methyl esterified, extracted with n-hexane, and then fatty acid. Used as analytical sample. At this time, the analysis condition of GC was SP-2560 (100 m × 0.25 mm, film thickness 0.20 μm), and the detector was a flame ionization detector (FID). The initial temperature of the column was 140 ° C. and maintained for 5 minutes, and then maintained at 4 ° C./min to 240 ° C. for 19 minutes. The temperature of the injector and detector was 260 ° C, the carrier gas was N 2 , and the flow rate was 0.8 mL / min. Each fatty acid was identified by comparing the retention time with the standard fatty acid methyl ester mixture (supelco 37 comp FAME Mix 10mg / ml in CH 2 Cl 2 ) under the same conditions.
(g) 총 식이섬유(g) total dietary fiber
총 식이섬유 분석은 소화효소에 의한 비소화성 잔사의 측정법 Prosky 법(AOAC 법)에 따라 동결 건조된 비빔밥을 분쇄하여 1 g을 정확히 측량하여, 500 mL 비이커에 취한 후, 여기에 0.08M Phosphate buffer(pH 6.0) 50 mL를 첨가하였다. 이 용액의 pH를 측정하여 산성이면 0.275N NaOH로, 알칼리면 0.325M HCl을 이용하여 pH 6.0±0.2가 되도록 조절하였다. 여기에 0.1 mL Termamyl(heat stable α-amylase) solution을 첨가한 다음, 알루미늄 호일로 비커를 덮어 95℃ 진탕항온수조(shaking water bath)에서 반응을 시켰다. 실온에서 이 용액을 30분간 방냉시킨 다음 0.275N NaOH용액 10 mL 넣어 pH 7.5±0.1로 조정하고 프로테아제 5 mg을 1 mL의 phosphate buffer에 넣어 조제한 프로테아제(protease) 용액 0.1 mL를 넣은 다음 다시 비커를 알루미늄 호일로 덮고 60℃ 인큐베이터에서 30분간 반응시켰다. 이 용액에 0.325M HCl 10 mL을 넣어 pH 4.0~4.6으로 조정하고 아밀로글루코시다아제(amyloglucosidase) 0.1 mL을 넣고 다시 비커를 알루미늄 호일로 덮고 60℃ 인큐베이터에서 30분간 반응시킨 후 실온에서 방냉 후 95% 에탄올을 용액의 4배 양인 285 mL 넣어준 후 침전시켰다. 0.5 g의 셀라이트(celite)를 유리 여과기(glass filter)에 담아 항량을 구한 수기를 상부 여과관(filter funnel) 위에 장치하고 78% 에탄올로 셀라이트(celite)를 고르게 하나의 막이 되도록 적신 후, 반응완료시킨 enzyme mixture를 흡인 여과시켰다. Total dietary fiber analysis was performed by measuring 1 g of lyophilized bibimbap, which was lyophilized according to the method of measuring non-digestible residues by digestive enzymes, and taken in a 500 mL beaker, followed by 0.08 M Phosphate buffer ( pH 6.0) 50 mL was added. The pH of this solution was measured and adjusted to pH 6.0 ± 0.2 using 0.275N NaOH if acidic and 0.325M HCl with alkali. 0.1 mL Termamyl (heat stable α-amylase) solution was added thereto, and the reaction was carried out in a shaking water bath at 95 ° C. by covering the beaker with aluminum foil. Allow the solution to cool at room temperature for 30 minutes, adjust to pH 7.5 ± 0.1 with 10 mL of 0.275N NaOH solution, add 0.1 mL of the prepared protease solution with 5 mg of protease in 1 mL of phosphate buffer, and then place the beaker Covered with foil and reacted for 30 minutes in an incubator at 60 ° C. Add 10 mL of 0.325M HCl to pH 4.0 ~ 4.6, add 0.1 mL of amyloglucosidase, cover the beaker with aluminum foil, and react for 30 minutes in an incubator at 60 ℃. % Ethanol was added to 285 mL of 4 times the amount of the solution and then precipitated. 0.5 g of celite was placed in a glass filter, and the obtained volume was placed on the upper filter funnel, and the celite was evenly soaked with 78% ethanol to form a single membrane. The reaction mixture was suction filtered.
여과가 완료되면 여기에 78% 에탄올 20 mL로 2회 세척한 뒤, 95% 에탄올 10 mL로 2회 세척하고 계속해서 아세톤 10 mL로 2회 세척하였다. 세척한 다음 침전물이 담긴 도가니는 105℃ 건조기에서 하룻밤 건조시킨 후 데시케이터에서 방냉한 후 무게를 측정하였다. 두 개의 시료 중 하나는 Kjeldahl법으로 단백질을 정량하고 나머지 한 개는 525℃에서 5시간 회화시켜 회분을 정량한 후 TDF 산출식에 적용하여 총 식이섬유 함량을 산출하였다. Once the filtration was completed, it was washed twice with 20 mL of 78% ethanol, then twice with 10 mL of 95% ethanol, and then with twice with 10 mL of acetone. After washing, the crucible containing the precipitate was dried overnight in a 105 ℃ dryer, cooled in a desiccator and weighed. One of the two samples quantified the protein by Kjeldahl method and the other one was incubated at 525 ° C for 5 hours to quantify the ash and applied to TDF formula to calculate the total dietary fiber content.
(h) 총 폴리페놀 화합물(h) total polyphenolic compounds
총 폴리페놀 함량은 Folin-Ciocalteu법으로 측정하였다. 분석을 위해 시료는 동결건조된 비빔밥을 곱게 갈아 0.5 g을 취하여 70% 에탄올을 이용하여 추출하였으며 최종 부피 50 mL로 정용하였다. 먼저 비빔밥 0.5 g을 70% 에탄올 15 mL을 혼합하여 30분 동안 초음파 처리(sonication)한 후 7,000 g에서 10분간(20℃) 원심분리한 후 여과하였고 이를 3반복하여 추출한 뒤 추출액을 50 mL로 정용하여 제조하였다. 이 추출액 0.1 mL과 증류수 3.9 mL, folin-ciocalteu 시약 0.5 mL을 혼합하여 5분간 실온에서 방치하였다. 방치한 용액에 포화 탄산나트륨용액 100 ul를 첨가하여 실온에서 30분간 반응시킨 후 725 nm 파장에서 흡광도를 측정하였다. 페놀함량을 표준물질로서 gallic acid를 사용하여 표시하였고, 전주 시내 비빔밥전문 음식점에서 판매되고 있는 시판 비빔밥(G社)을 비교구로 사용하였다.Total polyphenol content was measured by Folin-Ciocalteu method. For analysis, 0.5 g of the lyophilized bibimbap was finely ground, extracted with 70% ethanol, and the final volume was 50 mL. First, 0.5 g of bibimbap was mixed with 15 mL of 70% ethanol for 30 minutes, sonicated for 30 minutes, centrifuged at 7,000 g for 10 minutes (20 ° C), filtered, extracted three times, and extracted with 50 mL. It was prepared by. 0.1 mL of this extract, 3.9 mL of distilled water, and 0.5 mL of folin-ciocalteu reagent were mixed and left at room temperature for 5 minutes. 100 ul of saturated sodium carbonate solution was added to the solution, which was allowed to react at room temperature for 30 minutes, and then absorbance was measured at 725 nm. The phenol content was expressed using gallic acid as a standard, and a commercial bibimbap (G company) sold in Bibimbap restaurant in Jeonju city was used as a comparison.
(i) 항산화능(i) antioxidant activity
항산화 활성을 DPPH 자유 라디칼 소거능을 통해 확인하였으며 DPPH 자유 라디칼 소거능은 동결건조된 비빔밥을 곱게 갈아 0.5 g을 취하여 70% 에탄올을 이용하여 추출하였으며 최종 부피 50 mL로 정용하였다. 먼저 비빔밥 0.5 g을 70% 에탄올 15 mL을 혼합하여 30분 동안 초음파 처리(sonication)한 후 7,000 g에서 10분간 (20℃) 원심분리한 후 여과하였고 이를 3반복하여 추출한 뒤 추출액을 50 mL로 정용하여 제조하였다. 즉, 에탄올에 DPPH(1,1-diphenyl-2-picrylhydrazyl)를 용해하여 0.1 mM DPPH 용액을 준비하였다. 전처리 된 시료는 10,000 ppm의 농도로서 0.1 mM DPPH 용액과 섞어 37℃에서 30분 반응 후, 517 nm 파장에서 흡광도를 측정하고 시료 무첨가 대조구와 비교하여 아래 식에서 수소 공여능을 산출하였다.Antioxidant activity was confirmed by DPPH free radical scavenging ability, and 0.5 g of lyophilized bibimbap was extracted with 70 g of ethanol and extracted with 50 mL of final volume. First, 0.5 g of bibimbap was mixed with 15 mL of 70% ethanol for 30 minutes, sonicated for 30 minutes, centrifuged at 7,000 g for 10 minutes (20 ° C), filtered, extracted three times, and extracted with 50 mL. It was prepared by. That is, 0.1 mM DPPH solution was prepared by dissolving DPPH (1,1-diphenyl-2-picrylhydrazyl) in ethanol. The pretreated sample was mixed with 0.1 mM DPPH solution at a concentration of 10,000 ppm, and then reacted at 37 ° C. for 30 minutes, and the absorbance was measured at 517 nm. The hydrogen donating ability was calculated from the following equation compared with the sample-free control.
항산화능 표준물질로서 사용한 ascorbic acid는 항산화능 비교물질로 에탄올에 녹여 10,000 ppm으로 만들어 상기와 동일한 방법으로 처리하였고, 전주 시내 비빔밥전문 음식점에서 판매되고 있는 시판 비빔밥(G社)을 비교구로 사용하였다.Ascorbic acid, used as an antioxidant standard, was dissolved in ethanol as an antioxidant comparator and made into 10,000 ppm, and was treated in the same manner as above. A commercial bibimbap (G company) sold at a bibimbap restaurant in Jeonju city was used as a comparison.
DPPH free radical scavenging activity (%) ={1-(A-B/C)}× 100DPPH free radical scavenging activity (%) = {1- (A-B / C)} 100
A: 샘플 추출액과 DPPH를 반응시킨 후 흡광도 A: Absorbance after reacting the sample extract with DPPH
B: 샘플 추출액과 에탄올로 반응시킨 후 흡광도 B: Absorbance after reaction with sample extract and ethanol
C: 에탄올과 DPPH를 반응시킨 후 흡광도
C: absorbance after reacting ethanol and DPPH
(j) 항당뇨능(j) antidiabetic activity
분석을 위해 시료는 동결건조된 비빔밥을 곱게 갈아 0.5 g을 취하여 70% 에탄올을 이용하여 추출하였으며 최종 부피를 50 mL로 정용하였다. 먼저 비빔밥 0.5 g을 70% 에탄올 15 mL을 혼합하여 30분 동안 초음파처리(sonication)한 후 7,000 g에서 10분간(20℃) 원심분리한 후 여과하였고 이를 3 반복하여 추출한 뒤 추출액을 50 mL로 정용하여 제조하였다. 1,000 ppm의 비빔밥 시료는 100 μL안에 0.2 U/mL α-글루코시다아제 효소액과 100 mM 인산칼륨 완충용액(potassium phosphate buffer, pH 7.0) 그리고 100 mM 인산칼륨 완충용액(potassium phosphate buffer, pH 7.0)에 녹인 2.5 mM p-NPG(p-nitrophenyl α-glucopyranoside)를 가하여 37℃에서 20분간 반응시켰다. 405 nm에서 흡광도를 측정하고 시료 무첨가 대조구와 비교하여 아래식에서 α-글루코시다아제의 저해 활성능을 산출하였다. 항당뇨 표준물질로 사용한 acarbose는 D.W에 녹여 1 mg/ml로 만들어 상기와 동일한 방법으로 처리하였고, 전주 시내 비빔밥 전문음식점에서 판매되고 있는 시판 비빔밥(G社)을 비교구로 사용하였다.For analysis, 0.5 g of the lyophilized bibimbap was finely ground, extracted with 70% ethanol, and the final volume was adjusted to 50 mL. First, 0.5 g of bibimbap was mixed with 15 mL of 70% ethanol, sonicated for 30 minutes, centrifuged at 7,000 g for 10 minutes (20 ° C), filtered, and extracted three times, followed by extracting the extract to 50 mL. It was prepared by. 1,000 ppm bibimbap samples were prepared in 100 μL in 0.2 U / mL α-glucosidase enzyme solution, 100 mM potassium phosphate buffer (pH 7.0) and 100 mM potassium phosphate buffer (pH 7.0). 2.5 mM p-NPG (p-nitrophenyl α-glucopyranoside) was added thereto and reacted at 37 ° C. for 20 minutes. The absorbance was measured at 405 nm and the inhibitory activity of α-glucosidase was calculated by the following equation compared to the sample-free control. Acarbose, used as an antidiabetic standard, was dissolved in D.W to make 1 mg / ml and treated in the same way as above. A commercial bibimbap (G company) sold at a bibimbap restaurant in Jeonju city was used as a comparison.
α-글루코시다아제 저해 활성(%) ={1-(A-B/C)}× 100α-glucosidase inhibitory activity (%) = {1- (A-B / C)} × 100
A: 샘플 비빔밥과 α-글루코시다아제(enzyme)를 반응시킨 후 흡광도 A: Absorbance after reacting sample bibimbap with α-glucosidase
B: 샘플 비빔밥과 α-글루코시다아제-버퍼로 반응시킨 후 흡광도 B: Absorbance after reaction with sample bibimbap and α-glucosidase-buffer
C: α-글루코시다아제-버퍼와 α-글루코시다아제를 반응시킨 후 흡광도
C: absorbance after reacting α-glucosidase-buffer with α-glucosidase
(k) 항고혈압능(k) antihypertensive capacity
항고혈압 활성은 Angiotensin-converting enzyme(ACE)의 저해활성을 spectrophotometric assay 방법에 의해 측정하여 ACE 저해율로 표시하였다. 분석을 위해 시료는 동결건조된 비빔밥을 곱게 갈아 0.5 g을 취하여 70% 에탄올을 이용하여 추출하였으며 최종 부피를 50 mL로 정용하였다. 먼저 동결 건조된 비빔밥 분말 0.5 g을 70% 에탄올 15 mL을 혼합하여 30분 동안 초음파 처리(sonication)한 후 7,000 g에서 10분간(20℃) 원심분리한 후 여과하였고 이를 3 반복하여 추출한 뒤 추출액을 50 mL로 정용하여 제조하였다. 효소액 조제를 위해 0.3 M NaCl을 함유한 0.1M 인산칼륨 완충용액(potassium phosphate buffer, pH 8.3)에 토끼 폐 유래의 angiotensin converting enzyme (Sigma, USA)를 첨가하여 0.25 UN/ml이 되도록 용해하였다. HHL(hippuryl-histidyl-leucine)은 0.3M NaCl을 함유한 0.1M 인산칼륨 완충용액(potassium phosphate buffer, pH 8.3)에 용해시켜 2.5 mM HHL 기질용액을 조제하였다. Positive control로서 captopril(Biochemika, U.S.A.)을 사용하였고, 비빔밥 시료와 동일한 조건에서 비교하기 위해 1,000 ppm의 농도에서 분석하였다. Antihypertensive activity was measured by the spectrophotometric assay and the inhibitory activity of Angiotensin-converting enzyme (ACE) was expressed as ACE inhibition rate. For analysis, 0.5 g of the lyophilized bibimbap was finely ground, extracted with 70% ethanol, and the final volume was adjusted to 50 mL. First, 0.5 g of lyophilized bibimbap powder was mixed with 15 mL of 70% ethanol for 30 minutes, sonicated for 30 minutes, centrifuged at 7,000 g for 10 minutes (20 ° C), filtered, and extracted three times. Prepared by dialysis to 50 mL. To prepare the enzyme solution, an angiotensin converting enzyme (Sigma, USA) derived from rabbit lung was added to 0.1 M potassium phosphate buffer (pH 8.3) containing 0.3 M NaCl, and dissolved to 0.25 UN / ml. HHL (hippuryl-histidyl-leucine) was dissolved in 0.1 M potassium phosphate buffer (pH 8.3) containing 0.3 M NaCl to prepare 2.5 mM HHL substrate solution. Captopril (Biochemika, U.S.A.) was used as a positive control and analyzed at a concentration of 1,000 ppm for comparison under the same conditions as the bibimbap sample.
즉, 추출시료용액 100 uL에 기질액 hippuryl-histidyl-leucine(HHL) 150 uL과 0.25 UN/mL ACE 효소액 100 uL를 가한 다음 37℃에서 30분간 반응시켰다. 여기에 1N HCl 250 uL을 첨가하여 반응을 정지시킨 후, 에틸아세테이트 3 mL을 가해 1분 동안 교반하고, 이를 원심분리(5,000 x g, 10분, 4℃)하여 상등액 3 mL를 얻었다. 상등액을 120℃, 30분간 가열하여 완전히 건조시킨 후, 건조물은 2 mL의 증류수를 가하고 228 nm에서 흡광도를 측정하여 ACE 저해활성을 측정하였다. 대조구는 추출액 대신 추출용매를 동량 가해 실험하였으며, 동시에 시료 및 기질, 효소액을 첨가하고 반응 전에 1N HCl을 첨가하여 효소반응을 정지시킨 Blank군의 흡광도를 측정하였고, ACE 저해활성은 다음 식에 의하여 산출하였다.That is, 150 uL of substrate solution hippuryl-histidyl-leucine (HHL) and 100 uL of 0.25 UN / mL ACE enzyme solution were added to 100 uL of the extracted sample solution, followed by reaction at 37 ° C. for 30 minutes. After the reaction was stopped by adding 250 uL of 1N HCl, 3 mL of ethyl acetate was added thereto, stirred for 1 minute, and centrifuged (5,000 × g, 10 minutes, 4 ° C.) to obtain 3 mL of the supernatant. After the supernatant was heated to 120 ° C. for 30 minutes and completely dried, 2 mL of distilled water was added and the absorbance was measured at 228 nm to measure ACE inhibitory activity. The control group was tested by adding the same amount of the extraction solvent instead of the extract, and at the same time, the absorbance of the blank group was stopped by adding the sample, the substrate and the enzyme solution, and adding 1N HCl before the reaction, and the ACE inhibitory activity was calculated by the following equation. It was.
ACE inhibition (%) = (C-S / C-B) x 100ACE inhibition (%) = (C-S / C-B) x 100
S: 샘플 흡광도S: sample absorbance
C: control absorbance(시료 대신 추출용매 100 μL를 가해 반응시킨 대조구의 흡광도)C: control absorbance (absorbance of the control which was reacted by adding 100 μL of extraction solvent instead of the sample)
B: blank absorbance(효소반응 전에 0.1N HCl 용액을 첨가하여 효소반응을 정지시킨 Blank구의 흡광도)
B: blank absorbance (absorbance of blank spheres which stopped enzyme reaction by adding 0.1N HCl solution before enzyme reaction)
(l) 관능검사(l) sensory evaluation
기능성 식재를 이용한 비빔밥 개발의 기호도 검사를 위한 검사원은 (재)전주생물소재연구소의 연구원을 대상으로 20대에서 40대에 이르기까지 남성 6명, 여성 6명으로 총 12명을 선발하였다. 본 연구의 관능검사는 오전 10시부터 11시 사이, 또는 오후 4시부터 5시 사이에 관능검사를 실시하였고, 평가서는 시료의 배식과 함께 실험요원이 배부한 후 약 10분 후에 수거하였다. The inspector for the palatability test for the development of bibimbap using functional food was selected from 6 men and 6 women in their 20s and 40s for the researchers of Jeonju Institute of Biomaterials. The sensory evaluation was performed between 10 am and 11 am or between 4 pm and 5 pm. The evaluation was collected about 10 minutes after the distribution of the samples by the experimental personnel.
시료는 한 끼 식사의 절반량인 200 g에 맞추어 만들어 제공하였으며 온도는 65℃ 이상으로 따뜻하게 유지하여 제공하였다. 관능평가를 위해 검사원 각자에게 채점표를 나누어 주고 7점 척도에 의해 각 측정 항목의 기호도를 측정하도록 하였다. 각 메뉴특성에 대한 질문은 5문항으로 외관(색), 향, 맛, 입안에서의 느낌(질감), 전체적인 기호도 등 총 5가지의 항목에 대해 설명하였고, 척도는 7점 척도(1 : 매우 싫다, 4: 보통, 7: 매우 좋다)법을 사용하였다.
Samples were prepared for 200 g of half a meal and the temperature was maintained at 65 ° C. or higher. For the sensory evaluation, scorecards were distributed to each surveyor and the degree of preference of each item was measured by 7 point scale. The question about each menu's characteristics was five questions that explained five items, including appearance (color), aroma, taste, texture (texture) and overall preference, and the scale was a 7-point scale (1: very disliked). , 4: normal, 7: very good).
실시예Example 1: 비빔밥의 일반성분 1: General Ingredients of Bibimbap
기능성 식재를 이용한 본 발명의 항당뇨 비빔밥의 일반성분은 하기 표 2에 나타난 바와 같다. 수분함량은 73.88%를 나타내었으며, 조당질은 18.31%, 조단백질은 4.35%, 조지방은 2.41%, 조회분은 1.05%로 나타났고, 3대 영양소 중 조지방 함량이 가장 낮은 것으로 나타났다. General components of the anti-diabetic bibimbap of the present invention using a functional plant are as shown in Table 2 below. Moisture content was 73.88%, crude sugar was 18.31%, crude protein was 4.35%, crude fat was 2.41%, crude ash was 1.05%, and crude fat content was the lowest among the three major nutrients.
한국인 영양섭취 기준(2010)의 남자(30~49세)의 1일 열량 필요추정량은 2,400 Kcal, 1일 단백질 권장량은 55 g으로 이것의 1/3의 함량을 한끼당 열량 필요추정량 및 단백질권장량으로 산출하였을 때, 열량 필요추정량은 800 Kcal, 단백질 권장량은 18.3 g이다. 기능성 식재를 이용한 비빔밥의 단백질, 지방 및 탄수화물 함량에 농촌진흥청에서 발간한 식품성분표에서 제시한 FAO/WHO에너지 환산계수(단백질 2.44 Kcal/g, 지방 8.37 Kcal/g, 탄수화물 3.57 Kcal/g)를 곱하여 산출하였을 때, 항당뇨 식재를 이용한 비빔밥의 1인분 열량은 각각 384 Kcal로 한국인 남자(30~49세)의 한끼당 열량 필요추정량의 48.0%에 해당하고, 단백질 함량은 17.4 g으로 한국인 남자(30~49세)의 한끼당 단백질 권장량의 95.1%에 해당하여 저열량 고영양 식품임을 확인할 수 있었다.The estimated daily calorie requirement for men (30-49 years old) is 2,400 Kcal and the recommended daily protein amount is 55 g, according to the Korean Nutrition Intake Standard (2010). When calculated, the required calorie estimate is 800 Kcal and the recommended protein is 18.3 g. The FAO / WHO energy conversion coefficient (protein 2.44 Kcal / g, fat 8.37 Kcal / g, carbohydrate 3.57 Kcal / g) presented in the food composition table published by RDA for the bibimbap content of functional foods When multiplied, 1 serving of bibimbap with anti-diabetic foods was 384 Kcal, respectively, corresponding to 48.0% of the estimated calories per meal of Korean males (ages 30-49) and protein content of 17.4 g (Korean males). 30 ~ 49 years old, 95.1% of the recommended protein per meal was confirmed that it is a low-calorie, high-nutrition food.
항당뇨 식재를 이용한 비빔밥의 각 영양소가 차지하는 전체 열량에 대한 비율은 각각 탄수화물 68.0%, 지방 21%, 단백질 11%로 한국인 영양섭취기준 중 19세 이상 성인의 에너지 적정비율(탄수화물 55~70%, 단백질 7~20%, 지질 15~25%)과 비교 시, 비교적 부합하였다.
The ratio of total calories of each nutrient of bibimbap using antidiabetic ingredients was 68.0%, 21% fat, and 11% protein, respectively. The ratio of energy for adults aged 19 or older (carbohydrate 55 ~ 70%, 7-20% protein, 15-25% lipid).
실시예Example 2: 비빔밥의 미량성분 2: Trace Ingredients of Bibimbap
본 발명의 비빔밥의 비타민 C의 함량을 분석한 결과, 40.60 mg%로 나타났다. 비빔밥 1인 분량(400 g)을 기준으로 하였을 때, 항당뇨 식재를 이용한 비빔밥의 비타민 C 함량은 42.4 mg으로 성인(30~49세) 1일 권장섭취량 100 mg의 42.4%에 해당하여 비타민 C의 우수한 공급원임을 확인할 수 있었다. 또한, 본 발명의 비빔밥의 무기질 함량분석 결과, 총 무기질 함량은 비빔밥(DM basis) 100 g당 1,546.31 mg이였으며, 칼슘(Ca)은 102.77 mg, 인(P)은 189.63 mg, 나트륨은 603.54 mg, 칼륨 635.36 mg 및 철 15.01 mg의 함량을 나타내었다.As a result of analyzing the content of vitamin C of the bibimbap of the present invention, it was found that 40.60 mg%. Based on 1 serving of bibimbap (400 g), the vitamin C content of bibimbap using antidiabetic foods was 42.4 mg, which is equivalent to 42.4% of the recommended daily intake of 100 mg for adults (30 ~ 49 years). It was confirmed that it is an excellent source. In addition, as a result of analyzing the mineral content of the bibimbap of the present invention, the total mineral content was 1,546.31 mg per 100 g of bibimbap (DM basis), 102.77 mg of calcium (Ca), 189.63 mg of phosphorus (P), 603.54 mg of sodium, A content of 635.36 mg potassium and 15.01 mg iron was shown.
무기질(mg/100g)
Mineral (mg / 100g)
본 발명의 비빔밥에는 약 20여 종의 유리 아미노산이 검출되었으며, 유리아미노산 함량은 총 8.824 mg/g으로 나타났다. 한편, 대체적으로 트립토판(Tryptophane), 글루탐산(glutamic acid), 아스파르트산(aspartic acid), 아르기닌(arginine)이 주요 아미노산으로 나타났으며, 인체에 필요한 필수아미노산인 이소류신(isoleucine), 류신(leucine), 라이신(lysine), 메티오닌(methionine), 페닐알라닌(phenylalanine), 트레오닌(threonine) 그리고 발린(valine) 등은 전반적으로 골고루 분포되어 있었다.About 20 free amino acids were detected in the bibimbap of the present invention, and the free amino acid content was 8.824 mg / g. On the other hand, tryptophane, glutamic acid, aspartic acid, and arginine were shown as major amino acids, and essential amino acids such as isoleucine, leucine, and leucine Lysine, methionine, phenylalanine, threonine and valine were evenly distributed throughout.
본 발명의 비빔밥의 주요 지방산 조성비를 분석한 결과는 표 5와 같다. 주요 지방산은 리놀레산(linoleic acid), 올레산(oleic acid), 스테아린산(stearic acid), 팔미트산(palmitic acid)으로 나타났는데, 리놀레산(linoleic acid) 함량이 가장 높았으며, 필수지방산 함량도 비교적 골고루 분포되어 있었다.The results of analyzing the main fatty acid composition of the bibimbap of the present invention are shown in Table 5. The major fatty acids were linoleic acid, oleic acid, stearic acid, and palmitic acid, which had the highest content of linoleic acid and even distribution of essential fatty acids. It was.
실시예Example 3: 비빔밥의 특수성분 및 3: special ingredients of bibimbap and 항당뇨Anti-diabetic 활성 activation
본 발명의 비빔밥의 총 식이섬유 함량은 비빔밥 100 g 당 11.31 g으로 한끼의 비빔밥으로 1일 충분 섭취량의 상당부분을 충족시킬 수 있음을 알 수 있었다. 또한, 총 폴리페놀 함량은 시판 비빔밥에 비해 본 발명의 비빔밥이 더 높게 함유되어 있음을 확인할 수 있었으며, 또한, 항산화 활성, 항당뇨활성, 항고혈압 활성도 시판되는 비빔밥에 비해 본원 발명의 비빔밥이 높은 활성을 나타내는 것을 확인할 수 있었다.The total dietary fiber content of the bibimbap of the present invention was 11.31 g per 100 g of bibimbap, it can be seen that one meal of bibimbap can satisfy a substantial portion of sufficient intake per day. In addition, it was confirmed that the total polyphenol content of bibimbap of the present invention is higher than that of commercial bibimbap, and also, the bibimbap of the present invention has higher activity of antibiotic, antidiabetic and antihypertensive activity than commercial bibimbap. It could be confirmed that it represents.
실시예Example 4: 비빔밥의 관능평가 4: sensory evaluation of bibimbap
본 발명의 비빔밥의 관능 특성을 분석하기 위해 외관(색), 향, 맛, 질감 및 전체적인 기호도를 7점 척도법에 의해 평가하였다. 이때의 채점기준은 “아주 좋다”는 7점, “보통”은 4점, “아주 나쁘다”는 1점으로 하였고, 그 결과는 표 7에 나타내었다.In order to analyze the sensory properties of the bibimbap of the present invention, the appearance (color), aroma, taste, texture and overall acceptability were evaluated by a seven-point scale method. The scoring criteria at this time were “very good”, 7 points, “normal” was 4 points, and “very bad” was 1 point, and the results are shown in Table 7.
그 결과 본 발명의 비빔밥은 색, 향, 맛, 질감 및 전체적인 기호도의 모든 항목에 걸쳐서 5점 이상을 점수를 나타내어, 전반적인 기호도가 좋음을 시사한다고 판단된다.As a result, the bibimbap of the present invention indicates a score of 5 or more points over all items of color, aroma, taste, texture, and overall acceptability, and thus, suggests that the overall acceptability is good.
Claims (4)
(a) 현미와 메밀을 8~10:0.5~1.5의 중량비율로 혼합한 후 씻어 현미 메밀밥을 짓는 단계;
(b) 멸치육수에 청국장, 천일염 및 다진마늘을 혼합한 후 끓여 청국장 소스를 제조하는 단계;
(c) 곤약, 브로콜리 및 두부를 각각 끓는 물에 데쳐 준비하는 단계;
(d) 도라지, 표고버섯, 고사리 및 양파를 각각 볶아 준비하는 단계;
(e) 무채와 데친 시금치 각각에 양념을 하여 준비하는 단계; 및
(f) 상기 (a)단계의 현미메밀밥 160~200 중량부에 상기 (b)단계의 제조한 청국장 소스 30~50 중량부, 상기 (c)단계의 준비한 곤약 16~24 중량부, 브로콜리 16~24 중량부 및 두부 16~24 중량부, 상기 (d)단계의 준비한 도라지 16~24 중량부, 표고버섯 16~24 중량부, 고사리 16~24 중량부 및 양파 16~24 중량부와 상기 (e)단계의 준비한 무채 16~24 중량부 및 시금치 16~24 중량부를 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 항당뇨 활성이 우수한 비빔밥의 제조방법.The method of claim 1,
(a) mixing brown rice and buckwheat at a weight ratio of 8 to 10: 0.5 to 1.5 and washing to make brown rice buckwheat rice;
(b) mixing the anchovy broth with Cheonggukjang, sun-dried salt and chopped garlic and then boiling to produce Cheonggukjang sauce;
(c) preparing konjac, broccoli and tofu by boiling each in boiling water;
(d) preparing roasted bellflower, shiitake mushrooms, ferns and onions, respectively;
(e) seasoning and preparing each of the radish and poached spinach; And
(f) 160 to 200 parts by weight of brown rice buckwheat in step (a) 30 to 50 parts by weight of the prepared soybean paste sauce in step (b), 16 to 24 parts by weight of konjac prepared in step (c), broccoli 16 ~ 24 parts by weight and 16 to 24 parts by weight of tofu, 16 to 24 parts by weight of the bellflower prepared in step (d), 16 to 24 parts by weight of shiitake mushrooms, 16 to 24 parts by weight of fern and 16 to 24 parts by weight of onion ( e) 16 to 24 parts by weight of prepared radish and 16 to 24 parts by weight of spinach prepared in the step of producing a bibimbap excellent antidiabetic activity, characterized in that it comprises the step of manufacturing.
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Cited By (5)
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KR101526545B1 (en) * | 2015-01-20 | 2015-06-09 | 박금순 | Cheonggukjang rice and manufacturing method thereof |
KR101675571B1 (en) * | 2015-05-15 | 2016-11-11 | 농업회사법인 유한회사 농정심 | Manufacturing method of Bibimbab comprising natural topping and herb source |
KR101705022B1 (en) * | 2016-02-19 | 2017-02-09 | 재단법인 발효미생물산업진흥원 | Method for producing Ssamjang using fermented tomato product with improved antidiabetic activity and Ssamjang produced by the same method |
KR20190094572A (en) | 2018-02-05 | 2019-08-14 | 동의대학교 산학협력단 | Composition for anti-diabetic and manufacturing method thereof |
KR20190094573A (en) | 2018-02-05 | 2019-08-14 | 동의대학교 산학협력단 | Functional food composition for diabetic patients comprising barley and manufacturing method thereof |
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KR20040060530A (en) * | 2002-12-30 | 2004-07-06 | 강창환 | A boiled rice with assorted mixtures of fresh vegetables effective against diabets and its manufacturing method |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
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KR101526545B1 (en) * | 2015-01-20 | 2015-06-09 | 박금순 | Cheonggukjang rice and manufacturing method thereof |
KR101675571B1 (en) * | 2015-05-15 | 2016-11-11 | 농업회사법인 유한회사 농정심 | Manufacturing method of Bibimbab comprising natural topping and herb source |
KR101705022B1 (en) * | 2016-02-19 | 2017-02-09 | 재단법인 발효미생물산업진흥원 | Method for producing Ssamjang using fermented tomato product with improved antidiabetic activity and Ssamjang produced by the same method |
KR20190094572A (en) | 2018-02-05 | 2019-08-14 | 동의대학교 산학협력단 | Composition for anti-diabetic and manufacturing method thereof |
KR20190094573A (en) | 2018-02-05 | 2019-08-14 | 동의대학교 산학협력단 | Functional food composition for diabetic patients comprising barley and manufacturing method thereof |
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