KR101288540B1 - Composition of acaricidal components containing Lycopus lucidus TURCZ essential oil or Octene's derivatives and acaricide using the same - Google Patents

Abstract

The present invention relates to an acaricidal composition comprising an octane essential oil extract, a 1-octen-3-ol or a derivative thereof purified from the lanthanum essential oil extract, and a tick acaricide using the same. While having excellent acaricide activity against food mites, it has an effect as an eco-friendly sanction that is harmless to humans and has proven its stability.

Description

Composition of acaricidal components containing Lycopus lucidus TURCZ essential oil or Octene's derivatives and acaricide using the same}

The present invention relates to an acaricidal composition comprising taxane essential oil extract, 1-octen-3-ol or a derivative thereof purified therefrom, and a tick acaricide using the same.

Recently, as society becomes more complicated and industrialization progresses rapidly, environmental pollution is intensifying, and various diseases are emerging. Among such diseases, allergic diseases are not easy to identify the cause compared to the frequency of occurrence, causing many problems.

The mechanism of occurrence of these allergic diseases is classified into genetic and environmental factors, and they are represented by their complex immune response. Among these genetic factors, current modern medical technology can not control, but environmental factors can be allergic diseases caused by removing allergens that cause allergic diseases can be treated and prevented. In other words, by controlling external factors, it is possible to suppress the progress and development of allergic diseases.

However, the problem is that the types of allergens for these have not yet been accurately identified.

On the other hand, more than 70% of all pediatric respiratory disease patients and more than half of adult patients show positive allergic skin reaction test against house dust mite antigen. In other words, in allergic diseases such as asthma, atopic dermatitis, and allergic rhinitis, exposure and sensitization to house dust mite are important factors in addition to individual genetic susceptibility.

Their distribution is also influenced by various factors such as geopolitical location, climate, lifestyle, house structure, and degree of modernization and industrialization. In recent years, more people have been living in multi-family homes such as apartments and residential complexes, which are less heated and less well ventilated than conventional single-family homes, and house dust mites have been changed to Western lifestyles such as Western bedding, carpets, and humidifiers. It was a good place to live.

House dust mites that are commonly identified in Korea are Dermatophagoides belonging to the family Pyroglyphidae . farinae ) and vertical dust mites ( Dermatophagoides) pteronyssinus ), of which D. farinae is the dominant species. Other ticks are called stored food mites.

Stored food mites are commonly found in warehouses, cereals, and stored foods where food or grains are stored, and the representative species is the long hair mite ( Tyrophagus putrescentiae ). Long hair mite is also known as an important causative antigen in allergic diseases such as asthma, and has been reported to live more actively in food stores with high humidity.

Therefore, studies on how to effectively control such mites have been actively conducted in recent years, such as the way to inhabit the surface of the bed and physically inhale, remove various harmful mites. In addition, a chemical synthetic acaricide and an allergen-modifying agent, which are mainly composed of chemical substances such as benzyl benzoate, permethrin, and tannic acid, may be used for tick abrasion.

However, these chemical synthetic acaricides have a short period of activity after treatment, requiring repeated treatment, which causes many problems such as the appearance of resistant tick species. Therefore, a lot of research is being carried out around the world, focusing on the development of new forms of natural acaricides through eco-friendly materials. However, studies on secondary metabolites of plants that exhibit toxicity, inhibition, avoidance, growth control and behavioral control against specific pests are still insufficient, and further studies on them are urgently needed.

Accordingly, the present inventors, while continuing the effort to overcome the problems of the conventional synthetic acaricides related to ticks and to develop more environmentally friendly materials, as in the present invention, two house dust mites and one stored food 1-octen-3-ol (1-octen-3-ol) and its derivative compounds, which have excellent acaricidal activity against mites and are harmless to humans and have been proved to be stable, are petroleum essential oil extracts or substances isolated and purified from them. The acaricide composition and the acaricide containing it were completed.

Accordingly, it is an object of the present invention to provide an acaricidal composition comprising taxane essential oil extract, 1-octen-3-ol or derivatives thereof purified from taxane essential oil extract, and a tick acaricide using the same.

As one aspect for achieving the above object, the present invention is a lanthanum ( Lycopus lucidus ) 1-octen-3-ol (1-octen-3-ol) or its derivative compound 3,7-dimethyl-1-octen-3-ol (3, 7-dimethyl-1-octen-3-ol), 1-octen-3-yl acetate, 1-octen-3-yl butyrate ) And 3,7-dimethyl-1-octene (3,7-dimethyl-1-octene) provides a non-active composition comprising at least one compound selected from the group as an active ingredient.

The present invention also provides a tick acaricide prepared using the acaricide composition.

The acaricidal composition comprising the lanthanum essential oil extract or the octene derivative according to the present invention has excellent acaricide activity against two kinds of house dust mites and one kind of stored food mite, and is an environmentally friendly agent that has been proved to be safe and stable to humans. It has an effect.

1 is an EI-MS graph showing that the formula (1) of J33131 isolated and purified from taxane essential oil according to the present invention is 1-octen-3-ol (1-octen-3-ol).
Fig. 2 is a ¹ H-NMR graph showing that the formula (1) of J33131 isolated and purified from taxane essential oil according to the present invention is 1-octen-3-ol (1-octen-3-ol).
Fig. 3 is a ¹³ C-NMR graph showing that the formula (1) of J33131 isolated and purified from taxane essential oil according to the present invention is 1-octen-3-ol (1-octen-3-ol).

As one embodiment for achieving the above object, the present invention is a 1-octen-3-ol (1-octen-3-ol) or derivative compounds thereof isolated and purified from lycran ( Lycopus lucidus ) essential oil extract, taxane essential oil extract Phosphorus 3,7-dimethyl-1-octen-3-ol (3,7-dimethyl-1-octen-3-ol), 1-octen-3-yl acetate, 1 At least one compound selected from -octen-3-yl butyrate and 3,7-dimethyl-1-octene as an active ingredient It provides an acaricide composition comprising.

In the present invention, 'talan ( Lycopus lucidus ) 'is usually named because it grows around ponds and is also known as Jisun because it can eat roots. In Korea, it is easy to cope with nectar ( Lycopus lucidus Turcz.) is called the ground before flowering, and in China the leafy subfamily ( Lycopus) lucidus Turcz. var. hirtus Regel ). Talanchoe has no smell, taste is very bitter, and its properties are slightly warm. It is used for amenorrhea, menstrual pain, postpartum abdominal pain and bruise caused by fish blood, and is effective for boil, liver dysfunction and postpartum urination. Appearance is node, outer surface is yellowish brown or purple, and there is a seed ball on each leaf surface, the quality is light and easy to break, and the curved surface has white water quality and hollow shape.

In the present invention, 'essential oil' refers to a volatile oil having a strong aroma obtained from a flower, a bud, a leaf, a stem, a root, a natural resin, or the like of a plant. The main components are mono and sesquiterpene hydrocarbons polymerized by isoprene and terpene compounds such as alcohols, aldehydes, ketones and esters derived therefrom. Currently, there are more than 1,500 essential oils, about 100 of which use natural and synthetic flavors as raw materials.

Bioassay results of the essential oil, the check box according to the present invention, house dust mites and storage mites food exhibited a 100% live inert to each ^{5㎍ / ㎝ 2, 20㎍ / ㎝} 2 concentration (see Table 1).

On the other hand, in the present invention, the essential oil component extracted from the taxane is to use a conventional conventional, essential oil extraction from natural plants, which is continuous steam distillation method, compression method, reflux cooling extraction method, ultrasonic extraction method, solvent extraction method, It is carried out by any one method selected from among methods such as cold immersion method and supercritical carbon dioxide extraction method, and is performed by the method of continuous steam distillation method which is generally used.

That is, the talan essential oil in the present invention refers to a talan essential oil extract obtained by extracting talan by the continuous steam distillation method. 1-octen-3-ol (1-octen-3-ol), a substance isolated and purified as described above, is obtained from taxane essential oil extract.

In the present invention, the 'continuous steam distillation method', which is an essential oil extraction method, is as follows.

First, the dried plant is pulverized to a predetermined size, and then a predetermined amount of the crushed sample and distilled water are stirred and then injected into the extractor. At this time, distilled water is generally used 10 times the crushed sample. Then, the extraction solvent is injected into the plant. The extraction solvent may be an organic solvent or a mixed solvent thereof, and the organic solvent may be used including any one of lower alcohols having 1 to 5 carbon atoms, methanol, ethanol, isopropanol, acetone, ethyl acetate, and hexane, which is a nonpolar solvent. . The content of the extraction solvent may be appropriately adjusted according to the type of solvent selected and the sample, but preferably 3 to 10 times when water and lower alcohol are used, and 0.6 to 1 times when organic solvent is used. Thereafter, the sample group injected into the extractor is extracted at 10 to 100 ° C., the extraction solvent group is extracted at 10 to 50 ° C. to extract essential oils contained in the plant. Then, in order to separate essential oils in the plant contained in the extraction solvent, dehydrated and filtered by using a dehydrating agent such as anhydrous magnesium sulfate, anhydrous calcium sulfate or anhydrous silica gel, and extracting the taxane essential oil extract at a temperature of 0 to 25 ° C. Concentrate.

In addition, in the step of obtaining the essential oil extract in the talanch according to the present invention, the talc is air-dried for 24 to 48 hours in the shade, and then used to be ground to 10 to 100 mesh (mesh).

In the present invention, the '1-octen-3-ol (1-octen-3-ol)' derivative is a derivative of 1-octen-3-ol (1-octen-3-ol) represented by the formula (1). Can be used.

In the compounds of the following formulas (1) to (5), formula (1) is 1-octen-3-ol (1-octen-3-ol), and formula (2) is 3,7-dimethyl-1-octene- 3-ol (3,7-dimethyl-1-octen-3-ol), formula (3) is 1-octen-3-yl acetate, formula (4) is 1- Octen-3-yl butyrate, formula (5) is 3,7-dimethyl-1-octene (3,7-dimethyl-1-octene).

...화학식 (1)

... Formula (1)

...화학식 (2)

(2)

...화학식 (3)

(3)

...화학식 (4)

... Formula (4)

...화학식 (5)

... Formula (5)

3,7-dimethyl-1-octen-3-ol (3,7-dimethyl-1-octen-3-ol) which is a derivative of 1-octen-3-ol (1-octen-3-ol) in the formula ) [See Formula (2)], 1-octen-3-yl acetate [See Formula (3)], 1-octen-3-yl butyrate (1-octen-3 -yl butyrate) [See Formula (4)] and 3,7-dimethyl-1-octene [See Formula (5)]. have.

The compounds represented by the above formulas (1) to (5) show excellent acaricide effect against two kinds of house dust mites and one type of stored food mite, and the compounds represented by the formulas (1) to (5) alone or Two or more kinds can be mixed and used.

In addition, in the present invention, two kinds of house dust mites, large-legged dust mites, vertical patterned mites, and one kind of stored food mite for the validation of acaricide activity of 1-octen-3-ol and derivative compounds thereof through the examples. As a result of assaying for acaricide activity (carcinogenicity), it was confirmed that 1-octen-3-ol showed excellent acaricide activity as a whole (see Table 2).

On the other hand, in the present invention, 'lethal dose for 50% (LD ₅₀ )' refers to the dose or radiation dose of toxic substances that kill 50% of a group of experimental animals, in the case of toxic substances, It is expressed in mg of toxins per kilogram of body weight. In this case, the lethal dose varies according to the type of animal or the poisonous route (oral, transdermal or subcutaneous), so they are also booked. In addition, according to the US Environmental Protection Agency (1998) classification, half the lethal dose of oral administration is 500 to 5,000 mg / kg body weight (Class III), and it is a low toxicity substance, and 50 to 500 mg / kg body weight (Class II) is normal. It is classified as a toxic substance, and 1-50 mg (Class I) per kg body weight is classified as highly toxic.

According to the present invention, 1-octene-3-ol (1-octene-3-ol) was found to have a relatively low acute toxicity to mammals in oral toxicity experiments.

In the present invention, the taxane essential oil compound may be included in an amount of 0.01 to 40% by weight based on the total weight of the composition.

When the content ratio of the taxane essential oil compound is less than 0.01% by weight based on the total weight of the tick killing effect of the present invention may be small, when the content of more than 40% of the total weight, the relative content of other common compositions It may be difficult to show an appropriate acaricide effect in the blending ratio, and a problem may arise that the economics should be taken into account in the preparation of the acaricide.

On the other hand, the content of the taxane essential oil compound can be modified according to the formulation of the pesticide, the method of spraying, the area of spraying, the pesticide species.

In the present invention, the acaricide composition is two kinds of house dust mites ( Dermatophagoides farinae ), vertical patterned mite ( Dermatophagoides pteronyssinus ) or one kind of stored food mites ( Tyrophagus) putrescentiae ) can have a killing effect.

In the present invention, the large-legged dust mite, the vertical patterned mite is a kind of house dust mite, which is an insect of an arthropod belonging to the eight-legged arachnid. Eat dandruff, keratin, etc. falling from the body of humans, 1g of dandruff, keratin can be a month's food for thousands of house dust mites. They are very small, about 0.1 to 0.2 mm in size, and excreted by mites or carcasses of ticks are broken down into very small fine dust containing harmful proteins, which are dispersed in the air every time the mattress, urine, blanket and pillow are moved. When a person breathes easily enters the body and is mixed with dust becomes an allergen, which is the main cause of allergic asthma, rhinitis, atopic dermatitis, etc., there is a problem that is not easily removed by a general cleaner.

In the present invention, Tyrophagus putrescentiae , which is a long hair mite, is a species belonging to the genus Acaridae. It is a worldwide species that pollutes stored grains and processed foods. Usually they live on the surface of contaminated grain or food, but sometimes they penetrate inwards, causing serious economic losses, and causing allergies to farmers and workers who handle salted ham.

In another aspect, the present invention provides a tick acaricide prepared using the acaricide composition.

In the present invention, the mite acaricide may be used in the form of one or more formulations selected from the group consisting of liquids, emulsions, coatings, smokers, fumigants, granules and solids.

Acaricides containing taxane essential oils or octene derivative compounds separated therefrom may be used as a conventional method of treating pesticides, such as coating, dipping and fumigation, in which the dosage is also applied to a conventional pesticide. This is possible.

The acaricide may be prepared and applied in various forms, may be prepared in the form of a liquid, emulsion, coating or granules by dissolving or dispersing in one or more solvents for coating and dipping treatment, optionally using a surfactant for fumigation treatment In combination with a bulking agent, for example a liquid solvent, pressurized liquefied gas and / or a solid carrier, and are preferably used in the form of smokers, fumigants, coatings, granules and solids.

To prepare such various types of preparations, solvents and additives conventionally used in the art can be used for the taxane essential oil or the octene derivative compounds isolated therefrom according to the invention. For example, ethanol or water, which is harmless to the human body, may be prepared in a liquid form using a dispersion, and may be prepared in a solid form using a conventional excipient or the like. In addition, the concentration of the active ingredient in the acaricide is used in an amount effective to carry out acaricide mechanism with respect to the tick, the specific concentration at this time depends on the type of formulation and the method of application.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example  1. Extract essential oils from natural plants

Natural plant state talc ( Lycopus Lucidus ) outpost was washed three times in 1000 ml of distilled water and dried and pulverized for more than 48 hours in a well-ventilated place without sunlight.

150 ml of the pulverized sample was injected with 1500 ml of distilled water, and extracted by continuous steam distillation apparatus (Steam Distillation Extraction, SDE, Nickerson & Likens equipment) for 6 hours at 100 ° C. 300 ml was used.

The extracted hexane extract was dried over anhydrous magnesium sulfate as a desiccant, separated from the extraction solvent and the essential oil using a filter paper, concentrated under reduced pressure at 30 ° C., and sealed and refrigerated.

Example  2. of target mites Apoptosis  Bioassay

Example 2 -One. Breeding of ticks

The acaricide activity was assayed against two kinds of house dust mites and one kind of stored food mites of the lanthanum essential oil extracted from Example 1 at all times.

Two house dust mites and one stored food mite used in the present invention used mites bred under a relative humidity of 75 ± 5% and a temperature of 25 ± 1 ° C. in a laboratory where external exposure of any acaricide was blocked. In a 15 cm x 12 cm x 6 cm container, feed a 20: 1 mixture of pomace and ebiose with a tick and cover the lid of a 18 cm x 18 cm x 18 cm container with an appropriate amount of saturated aqueous sodium solution at the bottom. Breeding.

Example 2 -2. Apoptosis  Bioassay

The bioassay was carried out as follows to test the acaricide activity of the tick mites of the talan essential oil extracted in Example 1.

In the present invention, the acaricide activity against two kinds of house dust mites and one kind of stored food mite was performed by fumigant bioassay, which is a kind of indirect contact method. In the preliminary experiments, acetone, an organic solvent having no effect on the sample and the tick, was used as a treating solvent. A sample was set to 1 mg per 2 ml microtube, and each sample was dissolved in 20 μl of acetone for each concentration and treated with 10 μl on a paper disk.

After the treatment was dried for 10 minutes in the fume larynx and inserted into the microtube stopper, each adult tube was inoculated with 30 adult ticks of two kinds of house dust mites, one of the test mites and one stored food mite.

All experiments were carried out in three iterations, and bioassay results were observed by killing ticks using an optical microscope (x20) 24 hours after treatment.

At this time, the fine brush was used to stimulate the tick and the individual without any movement was considered to be fertilized. Live ratio was subjected to analysis of variance (ANOVA) after conversion to arcsine square-root value, significance was black in swepe (Scheffe's test, p = 0.05 , SAS Institute, 1996).

Salmon percentage extracted from taklan (mean ± standard deviation,%) essential oil Mite to be tested Salination rate (mean ± standard deviation,%) Treatment concentration ( μ g / cm ² ) 20 10 5 2.5 1.25 Talc Dust mites 100 100 100 70 ± 0.2 33 ± 0.5 Vertical pattern dust mite 100 100 100 65 ± 0.3 15 ± 0.4 Longhair mites 100 55 ± 0.2 25 ± 0.3 0 ± 0.0 0 ± 0.0

Bioassay results of talan essential oils for the two house dust mites and one stored food mite,

Dust mites showed 100% acaricide activity at low concentrations of 5 μg / cm ² , and stored mites showed 100% acaricide activity at concentrations of 20 μg / cm ² .

Example 3 . Acaricide  Separation and Purification of Substances

3-1. Silica gel column  Chromatography

After extracting the taxane essential oil in Example 1, the acaricide composition contained in the essential oil was isolated and purified. Talc essential oils, which showed excellent acaricide activity against two house dust mites and one stored food mite, were separated by silica gel column chromatography.

A solvent mixed with hexane and ethyl acetate in the ratio of 9: 1, 8: 2, and 7: 3 was used as the elution solvent, and fractions of J1, J2, J3, and J4 were obtained by fractionating 5 ml per minute. there was. As a result of assaying the acaricide activity of two kinds of house dust mites and one kind of stored food mite in each of the four fractions, the J3 fractionation layer showed excellent acaricide activity, and hexane and ethyl acetate were further refined. Secondary silica gel column chromatography was performed using an eluting solvent mixed at a ratio of 9: 1.

As a result, the fractions of J31, J32, J33 were obtained, and bioassay was performed, and the J33 fractionation had the best acaricide activity against ticks.

3-2. High Performance Liquid Chromatography

High performance liquid chromatography (HPLC) was performed to separate and purify the acaricide composition of a single substance from the J33 fractionation layer separated by silica gel column chromatography.

In order to isolate and purify the final acaricide active substance, the J33 fractionation layer was subjected to high performance liquid chromatography under conditions of maximum absorption wavelength of 251 nm, HPLC grade chloroform and detection rate of 3 ml / min. A fractionation layer of J333 was obtained.

The bioassay of each fractionation layer confirmed the excellent killing power in the J331 fractionation layer. In addition, the separation was performed by replacing the column for a smooth separation to confirm that J3311 as a single material.

3-3. Acaricide  Structural Analysis of Materials

The separated and purified J3311 fractionation layer was measured by mass spectrometry (EI-MS), and molecular weight was determined spectroscopically by ¹ H-NMR (600 MHz) and ¹³ C-NMR (150 MHz). Therefore, it was confirmed that the substance separated from the taxane essential oil was 1-octen-3-ol (1-octen-3-ol).

EI-MS (70eV) m / z (% relative intensity): M + 128 (100, base peak), 111 (11), 99 (12), 95 (11), 85 (5), 81 (20), 69 (19), 67 (11), 58 (31), 57 (76), 52 (10);

¹ H-NMR (CDCl ₃ , 600 MHz): 5.835-5.874 (1H, t , J = 23.4 MHz, H-2), 5.089-5.228 (2H, d , J = 83.4 MHz, H-1), 3.974-4.094 (1H, d , J = 72.0, H-3), 3.974-4.094 (2H, t , J = 72.0 MHz, H-4), 1.815 (1H, s , H-3), 1.318-1.323 (2H, d , J = 3.0 MHz, H-7), 1.295-1.306 (2H, d , J = 6.6 MHz, H-5), 1.295-1.306 (2H, d , J = 6.6 MHz, H-6), 0.878-0.901 (3H, doublet , J = 13.8 MHz, H-8);

¹³ C-NMR (CDCl ₃ , 150 MHz): 141.430 (C-2), 114.573 (C-1), 73.344 (C-3), 37.065 (C-4), 31.828 (C-6), 25.077 (C- 5), 22.674 (C-7), 14.095 (C-8)

...화학식(1)

(1)

Example  4. 1- Octene Of 3-ols and derivative compounds thereof Acaricide  compare

Validation of acaricide activity of the purified 1-octen-3-ol (1-octen-3-ol) and derivative compounds thereof in Example 3 was carried out through bioassay of two kinds of house dust mites and one kind of stored food mite. Table 4 below was compared with LD ₅₀ (half dose).

The 95% confidence band in Table 2 below indicates that the result can be trusted with a 95% probability.

Results of Measurement of Half-Resistance of Octene Compounds compound LD ₅₀ value ( μ g / cm ² )
(95% confidence) Dust mites Vertical pattern dust mite Longhair mites Talc oil 2.19
(2.13-2.25) 2.25
(2.00-2.50) 8.45
(7.80-9.10) 1-octen-3-ol 0.25
(0.22-0.28) 0.27
(0.26-0.28) 2.35
(2.21-2.49) 3,7-dimethyl-1-octen-3-ol 0.29
(0.28-0.30) 0.37
(0.36-0.38) 2.41
(2.32-2.50) 1-octen-3-yl acetate 2.42
(2.35-2.55) 2.48
(2.43-2.53) 5.80
(5.60-6.00) 1-octen-3-yl butyrate 2.32
(2.12-2.52) 2.36
(2.22-2.50) 5.20
(4.60-5.80) 3,7-dimethyl-1-octene 9.34
(8.68-10.00) 9.90
(8.80-11.00) - Benzyl benzoate 10.02
(9.98-10.06) 9.56
(9.50-9.62) 11.53
(11.04-12.02) Diet 36.84
(36.68-37.00) 18.23
(17.47-18.99) 30.33
(30.27-30.39)

In Table 2, 1-octen-3-ol (1-octen-3-ol) isolated and purified from taxane essential oils are two kinds of house dust mites, each of which is a target mite (large dust mite, vertical dust mite). The half lethal doses of and 1 stored food mite (long hair mite) were 0.25, 0.27, and 2.35 μ g / cm ² . Thus it can be seen that the excellent acaricide active material for three kinds of ticks.

As a result of performing bioassay on the derivative compound having a structure similar to 1-octen-3-ol (1-octen-3-ol),

3,7-dimethyl-1- containing methyl group (-CH ₃ ) at carbon position 3 and 7 in 1-octen-3-ol structure The half lethal doses of octen-3-ol (3,7-dimethyl-1-octen-3-ol) were 0.29, 0.37, 2.41 μg / cm ² .

This is slightly lower than 1-octen-3-ol, but compared with benzyl benzoate or DEET, which are currently commercially available organic synthetic acaricides. It can be seen that it has a very high killing power.

Next, in the 1-octen-3-ol (1-octen-3-ol) structure, the hydroxy group (-OH) of carbon 3 is lost and another functional group is attached to the 1-octene- modified into an alkene structure. 3-yl butylate (1-octen-3-yl butyrate) is 2.32, 2.36, 5.20 μg / cm ² and 1-octen-3-yl acetate is 2.42, respectively. 2.48, 5.80 μg / cm ² and 3,7-dimethyl-1-octene showed 9.34 and 9.90 μg / cm ² , respectively.

However, 3,7-dimethyl-1-octene showed no activity against long hair mites.

Example 5 . Stability Evaluation Data

1-octen-3-ol (1-octen-3-ol) is a substance registered in the Food Additives Database of the Food and Drug Administration (FDA). It has been known.

In addition, according to Material Safety Data Sheets (MSDS), 1-octen-3-ol (1-octen-3-ol) has an oral toxicity of 340 mg / kg and transdermal toxicity of 3,300 mg / kg. appear. Therefore, it was found that this substance has a relatively low acute toxicity to mammals.

However, the stability evaluation data for the semi-numerical dose of derivative compounds thereof have not been reported yet.

Claims (5)

1-octen-3-ol (1-octen-3-ol), 3,7-dimethyl-1-octen-3-ol (3,7-dimethyl-1-octen-3-ol), 1-octene- Acaricide composition comprising at least one compound selected from 3-yl acetate (1-octen-3-yl acetate) and 1-octen-3-yl butyrate as an active ingredient .
The method of claim 1,
The compound is characterized in that it comprises 0.01 to 40% by weight relative to the total weight of the composition, the non-active composition.
The method of claim 1,
The acaricide composition is a two-legged dust mite ( Dermatophagoides) farinae ), vertical dust mite ( Dermatophagoides) pteronyssinus ) or one of the stored food mites ( Tirophagus putrescentiae ), having an acaricide effect, characterized in that it has an acaricide effect.
Mite acaricides prepared using the acaricide composition of any one of claims 1 to 3.
5. The method of claim 4,
The acaricide is characterized in that it is used in the form of one or more formulations selected from the group consisting of liquids, emulsions, coatings, smokers, fumigations, granules and solids, mite acaricides.

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