KR101274257B1 - Composition for preventing alopecia and promoting hair growth containing essential oil from a seed of Ziziphus jujuba - Google Patents

Abstract

The present invention relates to a pharmaceutical composition for preventing hair loss and promoting hair growth, a health functional food composition and a cosmetic composition containing the jujube seed-derived essential oil extract as an active ingredient. Increasing the weight, thickness and hair follicle relative area has the effect of promoting hair growth.

Description

Composition for preventing alopecia and promoting hair growth containing essential oil from a seed of Ziziphus jujuba}

The present invention relates to a composition for preventing hair loss and promoting hair loss containing jujube seed-derived essential oil extract, more specifically, a pharmaceutical composition for promoting hair loss and promoting hair growth, containing a jujube seed-derived essential oil extract as an active ingredient, a health functional food composition, and It relates to a cosmetic composition.

Hair is one of the major parts of the body derived from the extracellular layer of the skin and is a body protective appendage, and is considered to be an accessory structure of the skin along with sebaceous glands, sweat glands and nails. Hair is also known as skin inducer because it derives from the skin during developmental growth. Hair is important for the overall appearance of the human body. Although some medical treatments have been developed, many suffer from hair loss or hair weakness. Therefore, the development of new treatments to prevent hair loss and to promote hair growth is an important situation.

In this regard, alternative medicines are of interest. Alternative medicine has not yet been incorporated into the mainstream of medical benefits because of limited scientific evidence and incomplete knowledge of the mechanism, but has emerged as an attractive solution worldwide. Although therapeutic studies have developed some synthetic drugs, the side effects associated with them cannot be overlooked. Herbal cosmetics are emerging as an appropriate solution to current problems, natural products are of interest in cosmetics, and about 1,000 plant extracts have been tested for hair growth, and are still broad and diverse in the future. It is a growing sector that is expanding rapidly.

Recently, great progress has been made in the study of hair growth promoters. Several animal models, including C3H mice, have been used to experimentally assess hair growth. Newer evaluation techniques based on cell cultures such as dermal dendritic cells, hair matrix cells and hairy root cells (ORSC) have been developed to quantitatively analyze the extent of hair growth in vitro . Among the cells constituting the hair tissue, dermal dendritic cells and ORSCs can be successfully cultured, but matrix cells have not been identified. Due to the complex structure of the hair tissue, even if epithelial or mesenchymal cells can be cultured individually, no clear results for hair growth will be immediately apparent.

A unique feature of hair growth is its volatility (growth, degenerative and rest periods). These cyclical changes include rapid remodeling of both the dermal and dermal components of the hair follicles. Dermal papilla (DP) is a major mesenchymal component, present at the deepest end of hair follicles, and is believed to play an essential role in the induction of new hair follicles and maintenance of hair growth.

Various cytokines and growth factors are involved in hair morphogenesis and hair growth regulation, which include epidermal growth factor (EGF), transforming growth factor-α (TGF-α) and TGF-β, keratin Keratin growth factor (KGF), insulin-like growth factor-1 (IGF-1), interleukin-1 (IL-1), basic fibroblast growth factor (basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), and hepatocyte growth factor (HGF). It has been found that dermal dendritic cell-inducing factors have a chemotactic effect on surrounding cells, leading to hair growth promotion.

Jujube fruit is described as the “fruit of life,” and the ancient Chinese have known its unique characteristics for thousands of years.Today's practitioners are looking for scientific evidence of the special characteristics of jujube. ) Is a kind of scientific name Ziziphus jujuba , a thorny rhamnaceous plant of the buckthorn family Rhamnaceae, and mainly uses the fruit.The exact natural distribution of the jujube is not clear due to its wide cultivation, It is thought to be northern India, southern China and central China, and according to the Wikipedia Encyclopedia, it is also distributed in southeastern Europe.The jujube fruit is edible, and various parts of the jujube ( Ziziphus jujuba ) have multiple pharmacological characteristics, for example contraception , has the analgesic and anti-diabetic properties. in some areas of the horn stem jujube (Ziziphus jujuba) Water is used to prevent pregnancy. However, scientific studies on the effect of Mr. jujube (Ziziphus jujuba) is a situation rarely. Is used to relieve Mr. insomnia and anxiety in the traditional medicine, jujube (Ziziphus jujuba). In the prior report Jujube ( Ziziphus jujuba ) has been reported to be effective in improving lipid composition in blood glucose and serum of dietary hyperlipidemic rats, especially Ziziphus jujuba as a therapeutic diet for the control of metabolic disorders in adult diseases. However, there are no reports of hair loss prevention and hair growth promoting activity of jujube ( Ziziphus jujuba ) seed-derived essential oil extract (Our paper: Kang Sun-cheol et. Al., Antioxidant and antilisterial effect of seed essential oil and organic extracts from Zizyphus jujuba, Food and Chemical Toxicology 47 (2009), 2374-2380).

Therefore, the present inventors have conducted intensive studies to develop hair loss prevention and hair growth promoting agent using natural products, and confirmed that the essential oil extract derived from jujube ( Ziziphus jujuba ) seed has hair loss prevention and hair growth promoting activity and the mechanism of the effect thereof. The present invention was completed by confirming.

Accordingly, an object of the present invention is to provide a pharmaceutical composition for preventing hair loss and promoting hair growth containing an extract of essential oil derived from jujube ( Ziziphus jujuba ).

In addition, another object of the present invention to provide a health functional food composition for preventing hair loss and promoting hair growth containing the extract of jujube ( Ziziphus jujuba ) seed derived essential oil.

It is another object of the present invention to provide a cosmetic composition for preventing hair loss and promoting hair growth containing an essential oil extract derived from jujube ( Ziziphus jujuba ).

An object of the present invention as described above is to separate the essential oil extract from the jujube seed, and after the local jujube treatment of the separated jujube seed essential oil extract to the epidermal skin of BALB / c mice, the hair length, weight, thickness and relative area of hair follicles increase It was achieved by identifying the effect of promoting hair growth by entraining.

The present invention provides a pharmaceutical composition for preventing hair loss and promoting hair growth, containing an essential oil extract derived from jujube ( Ziziphus jujuba ) seed.

In another aspect, the present invention provides a health functional food composition for preventing hair loss and promoting hair growth containing the extract of jujube ( Ziziphus jujuba ) seed derived essential oil.

In addition, another object of the present invention provides a cosmetic composition for preventing hair loss and promoting hair growth containing an essential oil extract derived from jujube ( Ziziphus jujuba ).

In the present invention, the term "active ingredient" refers to a substance or a group of substances (including medicinal herbs whose pharmacologically active ingredients are not known) which are expected to directly or indirectly express the efficacy effect of the medicine by inherent pharmacological action. It means containing a main component as.

In the present invention, "health functional food" means a food manufactured and processed using raw materials or ingredients having functional properties useful for the human body according to the Health Functional Food Act No. 6767, and "functional" means a human body It means the ingestion for the purpose of obtaining a useful effect in health use such as nutrient control or physiological action on the structure and function of.

Pharmaceutical compositions comprising the jujube seed essential oil extract of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.

Pharmaceutical compositions comprising the essential oil extract of jujube seed of the present invention, powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, oral formulations, external preparations, suppositories, and sterile injectable solutions, respectively, according to a conventional method. Formulated in the form of can be used.

Carriers, excipients and diluents that may be included in the pharmaceutical composition comprising the jujube seed essential oil extract of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate , Gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid preparations include at least one excipient such as starch, calcium carbonate, sucrose in the extract. Or lactose, gelatin, or the like is mixed. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The preferred dosage of the jujube seed essential oil extract according to the present invention depends on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and the duration, and may be appropriately selected by those skilled in the art. However, for the desired effect, the jujube seed essential oil extract of the present invention is preferably administered at 0.0001 to 100 mg / kg, preferably at 0.001 to 100 mg / kg. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

Jujube seed essential oil extract according to the present invention can be administered to various mammals such as mice, mice, livestock, humans. All modes of administration can be envisaged, for example, can be administered directly to the skin such as oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebroventricular injections and scalp.

In addition, jujube seed essential oil extract according to the present invention may be added to food or beverage for the purpose of hair loss prevention and hair growth promoting effect. Foods to which the jujube seed essential oil extract can be added include, for example, various foods, beverages, gums, teas, vitamin complexes, health functional foods, powders, granules, tablets, capsules or beverages. At this time, the amount of the jujube seed essential oil extract in the food or beverage may be added at 0.01 to 15% by weight of the total food weight, the health beverage composition is 0.02 to 5 g, preferably 0.3 to 1 g based on 100 ml Can be added in proportion.

The food composition of the present invention is not particularly limited to other ingredients except the jujube seed essential oil extract as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, natural and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extracts (e.g., rebaudioside, A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of natural carbohydrates is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.

In addition to the above, the jujube seed essential oil extract of the present invention can be used in various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors such as flavoring agents, coloring and neutralizing agents (such as cheese, chocolate), pectic acid and salts thereof, Alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the jujube seed essential oil extract of the present invention may contain flesh for preparing natural fruit juice and fruit juice beverage and vegetable beverage. These components can be used independently or in combination. The proportion of such additives is not so important, but the jujube seed essential oil extract of the present invention is generally selected in the range of 0 to about 20 parts by weight per 100 parts by weight.

In the cosmetic composition of the present invention, the composition is characterized in that the formulation of the lotion (skin lotion), lotion, cream, massage cream, essence, pack. The formulations may be prepared using methods known in the art. In addition, the content of the jujube seed essential oil extract in the formulation may also be included within the content range (about 1-10% by weight) conventionally known in the art.

Components included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the jujube seed essential oil extract as an active ingredient, for example, stabilizers, solubilizers, vitamins, pigments and flavorings Conventional adjuvants and carriers are included.

In the present invention, jujube seed was steam distilled to obtain a pale yellow oil mainly composed of phenolic compounds, oxygenated mono- and sesquiterpenes, and oils having their respective hydrocarbons.

The present invention focused on the scientific investigation into the hair growth potential of jujube seed essential oil extract in a dose dependent manner. Hair removal skin of BALB / c mice was treated with jujube seed essential oil extract and hydrocortisone for 7 days, and hair growth effects were confirmed for 21 days.

Hair growth was more significantly initiated in the 1% and 10% jujube seed essential oil extract treated hair removal areas compared to the control (see FIG. 1). This explains the fact that more hair follicles are present in the growth phase of the hair growth cycle in the 1% and 10% jujube seed essential oil extract treatments. This was confirmed again by measuring the hair length on days 7, 14 and 21 days after the experiment (see FIG. 2).

In order to further investigate the hair growth effect, in the present invention, the weight of hair per cm ² of back skin area was measured. As shown in Figure 3, the hair weight of the jujube seed essential oil extract treated mice was heavier than the control. However, hydrocortisone did not show any effect on hair growth. In the present invention, hair thickness and relative area of hair follicles were also evaluated using a microscope. As shown in Figures 4 and 5, 1% and 10% jujube seed essential oil extract treated mice in the assay showed a more substantial effect compared to the control and other spheres.

In conclusion, the present invention concluded that the jujube seed essential oil extract has hair growth promoting activity, and it is believed that the jujube seed essential oil extract may be included as a component of the hair growth promoter derived from natural resources.

As described above, the jujube seed essential oil extract according to the present invention has an effect of promoting hair growth by increasing hair length, weight, thickness, and hair follicle relative area in a depilation animal model, which is useful as a hair loss prevention and hair growth promoter.

1 is a diagram showing hair growth activity in hair removal-treated BALB / c mice.
Figure 2 is a diagram showing the results of hair length measurement in hair removal treatment BALB / c mice.
Figure 3 is a diagram showing the results of hair weight measurement in hair removal treatment BALB / c mice.
Figure 4 is a diagram showing the results of measuring the hair thickness in hair removal treatment BALB / c mice.
5 is a diagram showing the results of hair follicle area measurement in hair removal-treated BALB / c mice.

Hereinafter, preferred embodiments of the present invention will be described in more detail with reference to Examples. However, the scope of the present invention is not limited to these examples.

Example

Jujube seeds were collected in August 2008 in Gyeongsan, Korea, washed, dried and ground.

As experimental animals, 5-week-old female BALB / c mice (18-20 g) were purchased from Orient Bio, Inc. (Seoul, South Korea), and placed in polypropylene cages (3 per cage) in a standard laboratory. Diet and water were optionally fed. The experimental animals were bred for about 7 days before the experiment in a 12:12 hour contrast cycle in an air-conditioned room. Room temperature (about 23 ° C.) and humidity (about 60%) were automatically adjusted.

Statistical analysis was performed using one-way ANOVA. Statistical package (SPSS version 11.0) was used for data analysis.

Example 1 Isolation of Jujube Seed Essential Oil Extract

Approximately 250 g of crushed jujube seeds using a Klevenzer-type apparatus were steam distilled for 3 hours to obtain an essential oil extract as a pale yellow oil as an extract of an oil having phenolic compounds, oxygenated mono- and sesquiterpenes and their respective hydrocarbons. . The obtained essential oil extract was dried over anhydrous Na ₂ SO ₄ and stored at 4 ° C. in a sealed vial until further analysis.

Experimental Example 1: In vivo hair growth activity assay

Mice were divided into five groups of three each. All the mice's back hairs were shaved 4 cm ² using an electric shaver. 10 μL of the essential oil extract prepared in Example 1 and hydrocortisone were applied daily to the shaving site of each mouse, and no control was performed. This treatment lasted for 7 days. Hair growth was visually observed and recorded at 7, 14 and 21 days from the start of topical application.

Except for the hydrocortisone treated group, it was observed that hair growth was initiated at the shaving site at the end of the experiment in all groups. As shown in FIG. 1, it can be seen that the entire hair removal site of the jujube seed essential oil extract treated mice was covered with hair at the end of the 3rd week compared with the control group. In the hydrocortisone treated group, the hair appeared poorer than the control group.

Experimental Example 2 Hair Length Measurement

On the 7, 14 and 21 days after starting the treatment of Experimental Example 1, hair was randomly collected from the shaved areas of all mice. The length of 10 hairs was measured and the average length was calculated. The results are expressed as the average length ± S.D. of 10 hairs.

As shown in Figure 2, 1% and 10% jujube seed essential oil extract treated mice showed a greater effect on hair growth compared to the other groups. At the end of the experiment, the hair lengths of the 1% and 10% jujube seed essential oil extract treatment groups were found to be 9.96 and 10.02 mm, respectively, compared to the control (8.94 mm) and 0.1% jujube seed essential oil extract treatment groups (9.22 mm). lost. Hydrocortisone, on the other hand, did not show any effect on hair growth.

Experimental Example 3 Hair Weight Measurement

On the 21st day of starting the treatment of Experimental Example 1, the cervix of the mouse was displaced and killed. 1 cm ² of skin with the back of the hair and the back of the hair without hair was cut out from all mice in each group and weighed by a balance. After metering, the hair weight was calculated by subtracting the skin weight from the hairy skin weight. The results are expressed as the average length ± SD of 10 hairs.

Freshly grown hair was weighed in all test groups and compared to controls. Hair weight was found to be highest in mice treated with 1% jujube seed essential oil extract. Hair weight was measured at 53, 57 and 54 mg per cm ^{2 of} dorsal skin area, respectively, in 0.1, 1 and 10% jujube seed essential oil extract treated mice, whereas in control, 50 mg per cm ² of skin area (FIG. 3). Reference).

Experimental Example 4 Measurement of Hair Thickness and Hair Follicle Area

Hair was immediately taken from the shaved area of all mice and hair thickness and hair follicle area were evaluated microscopically (magnification × 400).

The diameter of the hair and the area of the hair follicles were calculated by the above photographs and expressed as the mean ± S.D. of 10 vibrissal hairs of each group.

As shown in FIG. 4, hair thickness was measured at each 5, 10 and 15 cm distance from the hair follicle using high magnification micrographs for each hair. The distance from the hair follicles was found to change hair thickness. Within 5 cm from the hair follicle, the hair thickness of the control group was almost the same as that of the control, whereas within 10 cm of the hair follicle, the hair thickness was significantly increased compared to the control. The 0.1%, 1% and 10% jujube seed essential oil extract treatments were 4.4, 5.0 and 4.8 cm compared to the control (4.1 cm), respectively. In contrast, hair thickness was 4.9, 5.1, 6.2 and 5.6 cm in the control, 0.1%, 1% and 10% jujube seed essential oil extract treatments, respectively. The relative area of hair follicles is shown in FIG. 5. In the present invention, the area of hair follicles was found to be larger in the 1% and 10% jujube seed essential oil extract treatment than in the control. On the other hand, hydrocortisone and 0.1% jujube seed essential oil extract treatment did not show an effect on the hair follicle area.

Claims (3)

A pharmaceutical composition for promoting hair growth containing an essential oil extract derived from jujube ( Ziziphus jujuba ) seed as an active ingredient.
A functional food composition for promoting hair growth comprising an essential oil extract derived from jujube ( Ziziphus jujuba ) seed.
A cosmetic composition for promoting hair growth comprising an essential oil extract derived from jujube ( Ziziphus jujuba ) seed.

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