KR101265728B1 - Method for increasing seed productivity and oil of flax plant using gibberellin biosynthetic inhibitors - Google Patents

Abstract

PURPOSE: A method for enhancing flax seed production and oil content using a gibberellin biosynthesis inhibitor is provided to increase flax seed production and oil content by spraying mepiquat chloride or trinexapac-ethyl at an optimal concentration. CONSTITUTION: A method for enhancing flax seed production and oil content comprises the following steps: seeding flax seeds; irrigating the soil with water after 40-45 days from the flax seeding; and spraying the phylloplane of a flax plant body with 200-600 ppm of chloride or 100-300 ppm of trinexapac-ethyl at a speed of 80 to 100 milliliters per 1 m^2. When the mepiquat chloride is sprayed, palmitic acid and stearic aid, which are saturated fatty acids, are decreased while concentration is increased, but oleic acid, linoleic acid, and linolenic acid content, which are unsaturated fatty acids, are increased. [Reference numerals] (1) Spraying time: 50 days after seeding; (2) Spraying amount: 1liter/10m^2; (3) Spraying method: phylloplane spraying using a spay; (4) Spraying concentration: 600ppm; (AA) Flax seeding(early in April, 30cm of inter row planting spacing, drill planting, seeding rates: 500g/10a); (BB) Irrigating operation(40-45 days after seeding, sufficiently irrigating into a soil layer); (CC) Manufacture an enhancer for the productivity and oil content of flax seeds(0.6g of mepiquat chloride + 500ul of a wetting agent + 1l of distilled water); (DD) Spray the enhancer for the productivity and oil content of flax seeds

Description

Method for increasing seed productivity and oil of flax plant using gibberellin biosynthetic inhibitors}

The invention is mepi quart chloride compounds which inhibit the biosynthesis of gibberellins in plant hormones that promote shoot growth of the plants; flax (Mepiquat chloride MC), or tree-neck sapak ethyl (Trinexapac-ethyl) to the optimum concentration (Linum usitatissimum L.) A method for increasing the seed yield and oil content of a plant by spraying it on the plant.

Maybe Linum usitatissimum L.) is a seed of flax plants native to the highlands of Central Asia, and is an economically important oilseed crop containing about 40% fat. The shape is flat and oval with brown and gold color. Flax oil has long been used for industrial purposes such as printing inks, watercolors, paints, linoleum, etc., but the role as a functional food has been important recently because of its excellent nutritional and pharmacological value. Flaxseed oil is known to contain numerous essential ingredients such as fat, protein, fiber, lignans, minerals and vitamins. In addition, flax oil has received much attention as a high content of α-linolenic acid (ALA; C18: 3n-3) and the effects on the health functionalities of omega-3 fatty acids have been reported. In addition, α-linolenic acid is the parent fatty acid of omega-3 fatty acids, and after ingestion, undergoes human metabolism and docosahexaenoic acid (DHA, C22: 6n-3) and eicosapentaenoic acid. (eicosapentaenoic acid) (EPA, C20: 5n-3). At the time when the development of foods with enhanced functionalities is being activated, flax oil is actively being researched as an omega-3 fatty acid source regarding potential effects on the prevention and treatment of cardiovascular diseases.

In addition, flax oil has been reported to have anti-inflammatory effects as a mediator for antioxidant activity, anti-tumor activity, cardiovascular protective action, antihyperlipidemia and the synthesis of eicosanoids. Despite these many advantages, domestic linseed seed and linseed oil are not very applicable to the food industry. Hydrogen cyanide (HCN), which is produced as a hydrolyzate of flax seed cyanide glycosides, is known to cause acute poisoning and chronic CNS syndrome such as Konzo in humans and animals. And can be used as raw materials when processing.

Mepiquat chloride, trinexapac-ethyl and ancymidol are among the representative plant growth inhibitors used in farms, especially in grape cultivation (Gebong, Black Olympia). It has been used as a growth regulator for reducing severity and wetting effort, and it is known as a very useful substance as a pesticide because it has little effect on useful insects such as bees and silkworms (Pesticide Use Manual; Pesticide Industry Association. 2002) ). In addition, cotton cultivation has been reported to increase yield. Therefore, the mepiquat chloride is known to affect the growth and differentiation of plants as a result of stem shortening of the plant, delayed formation of snowflake, suppression of germination and reduced yield.

Therefore, the present inventors have tried to increase the seed production and oil content of flax, and as a result, it was found that either mepiquat chloride (MC) or Trinexapac-ethyl was used before the flowering of flax plants (after sowing, 50 Foliar spraying from 80 ml to 100 ml per m ₂ at concentrations of 600 ppm and 300 ppm, respectively, in aqueous solutions, significantly increased the yield of flax seeds, the oil content of linseed seeds, and the linolenic acid content of unsaturated fatty acids. The present invention was completed by confirming the increase.

It is an object of the present invention to provide a production and oil content enhancer of flax seeds containing mepiquat chloride or Trinexapac-ethyl as an active ingredient.

It is another object of the present invention to provide a method for increasing the yield and oil content of flax seeds by spraying the mepiquat chloride or trinexapak ethyl at an optimal concentration.

In order to achieve the above object, the present invention maybe (Linum containing mepi quart chloride (Mepiquat chloride) or tree-neck sapak ethyl (Trinexapac-ethyl) as an active ingredient usitatissimum L.) Provides seed yield and oil content enhancers.

Further, according to the present invention,

1) sowing flax;

2) watering the soil 40 to 45 days after sowing; And

3) A method for increasing the yield and oil content of flax seeds, comprising spraying mepiquat chloride or trinexapac ethyl on flax plants 50 to 55 days after sowing.

Flax ( Linum ) containing mepiquat chloride or Trinexapac-ethyl of the present invention as an active ingredient usitatissimum L.) Seed production and oil content enhancers were planted in flax plants at 80 ml and 100 ml per m ₂ at concentrations of 600 ppm and 300 ppm, respectively, in aqueous solution before flowering (45-50 days after sowing). In case of foliar spraying, the production of flax seeds and the oil content of flax seeds are increased, and the content of linolenic acid, which is an unsaturated fatty acid, is significantly increased, and thus it can be usefully used to increase the production and oil content of flax seeds.

1 is a flow chart of a cultivation technique for increasing flax seed production, flax seed oil content and unsaturated fatty acid linolenic acid content.

Hereinafter, the present invention will be described in detail.

The present invention contains linpiquat chloride (Mepiquat chloride) or Trinexapac ethyl (Trinexapac-ethyl) as an active ingredient ( Linum usitatissimum L.) Provides seed yield and oil content enhancers.

The mepiquat chloride preferably has a concentration of 200 to 600 ppm in an aqueous solution, and more preferably 500 to 600 ppm, but is not limited thereto. When the mepiquat chloride is less than 200 ppm in aqueous solution, it does not effectively reduce the content of gibberellin and thus does not increase the yield of flax seed.

The trinexapak ethyl preferably has a concentration of 100 to 300 ppm in an aqueous solution, and more preferably has a concentration of 200 to 300 ppm, but is not limited thereto. When the trinexapak ethyl is less than 100 ppm in an aqueous solution, it does not effectively reduce the content of gibberellin and thus does not increase the yield of flax seed. It is preferable to spray, spray, atomize or foliate the flax plant at 80 to 100 ml per 1 m _2, and to improve the flax seed on flax plant at 100 ml per 1 m ₂ . Spraying is more preferred but not limited thereto. The cultivation technique that must be preceded before the production of the flax seed and the oil content enhancer application must be sufficient in the soil. Insufficient water in the soil significantly reduces the effects of gibberelup biosynthesis inhibitors, mepiquat chloride or trinexapak ethyl, which do not increase the yield of flax seeds.

The flax seed production and oil content enhancer preferably decreases the saturated fatty acid content and increases the unsaturated fatty acid content with respect to flax seed oil, and more preferably increases the content of linolenic acid.

The flax seed production and oil content enhancer preferably further includes an electrodeposition agent, and may include 0.1 to 2% by weight of a surfactant that serves as an electrodeposition agent in the flax seed production and oil content enhancer, but is not limited thereto.

As the surfactant, a nonionic surfactant is preferable, and for example, TWEEN-80 (PEO (20) -Sorbitan Monooleate), TWEEN-60 (PEO (20) -Sorbitan Monostearate), etc., which have been confirmed to be safe to the human body, may be used. Can be used.

In a specific embodiment of the present invention, the inventors of the selected linseed seed (variety name: Hwanamam, Gyeongsangbuk-do Agricultural Research and Extension Services) 500 g per 300 pyeong of the field at 30 cm intervals per week where the seeding depth in the soil is 1 cm. Then, water the soil 40 to 45 days after sowing, and then on day 50 after sowing, mepiquat chloride, a gibberellin biosynthesis inhibitor, and trinexapac ethyl, a representative plant growth inhibitor used in farms, Ethyl) was prepared by concentration and 1 liter per 10 m ² was evenly sprayed onto the plant using a sprayer. Seeds of flax were harvested from 80 to 110 days after sowing, and the cultivation method and the harvested seeds were used in the following experiments (see FIG. 1).

In another specific embodiment of the present invention, the present inventors have found that the plant length (cm), branch water (dog), pod water (dog), ripening ratio (%), and granule weight (g) of flax plants according to the spraying concentration of mepiquat chloride. In the case of TRINEX-PAKethyl, the grass height decreased as the concentration was increased, and the branch number was small at low concentration and increased at high concentration. The maturity ratio, which is an indicator of the fidelity of seeds, was significantly increased when compared with no treatment, and the yield of seeds was also increased. On the other hand, mepiquat chloride was found to decrease in height as the concentration was increased and the number of branches increased than Trinexapakethyl. In particular, it was found that the grain weight was heavier as the concentration was increased and the seed yield showed the highest yield from 600 ppm to 196 kg (see Table 1).

In another specific embodiment of the present invention, the present inventors measured the oil content of flax seed, iodine value indicating the fat maintenance characteristics and unsaturated ratios indicating the oil quality according to mepiquat chloride and Trinexapak ethyl spraying concentration. As a result, it was 382 g / kg, which was the highest at 600 ppm of mepiquat chloride, and 163 mg / g of iodine value, which indicates fat maintenance, was dry oil. In particular, the unsaturated ratio indicating the quality and value of oil was 13.3 at 600 ppm of mepiquat chloride, and it was confirmed that it had the best oil properties (see Table 2).

In another specific embodiment of the present invention, the inventors conducted fatty acid analysis of flax seeds according to mepiquat chloride and tricexacac ethyl sparging concentration, and when saturated spawn was applied to mepiquat chloride, While palmitic acid and stearic acid were decreased, the content of unsaturated fatty acids oleic acid, linoleic acid, and linolenic acid was increased, and the linolenic acid composition ratio was 61.3%, especially at 600 ppm of mepiquat chloride (Table 3).

Thus, Flax (Linum containing mepi quart chloride (Mepiquat chloride) or tree-neck sapak ethyl (Trinexapac-ethyl) according to the present invention as an active ingredient usitatissimum L.) Seed production and oil content enhancer 1 liter per 10 m ² of flax plants at concentrations of 200 to 600 ppm or 100 to 300 ppm, respectively, in aqueous solution before flowering (45 to 50 days after sowing). In case of foliar application, the linseed production and linseed oil content are increased, and the content of linoleic acid, which is an unsaturated fatty acid, is significantly increased. Therefore, flax seed production is increased, and oil and linolenic acid contents are increased. It can be usefully used.

Further, according to the present invention,

1) sowing flax;

2) watering the soil before flowering after sowing; And

3) A method for increasing the yield and oil content of flax seeds, comprising spraying mepiquat chloride or trinexapac ethyl on flax plants 50 to 55 days after sowing.

Linum of step 1) above usitatissimum L.) may be grown or marketed.

The seeding of step 1) is sown 400 to 600 g per 300 pyeong of the selected flax seeds at intervals of 25 to 35 cm weekly, sowing depth in the soil is preferably 0.8 cm to 1.2 cm, 300 selected seeds 500 g per square lined at 30 cm intervals per day, more preferably, sowing depth in the soil is 1.0 cm, but is not limited thereto.

It is preferable that it is 40-45 days after sowing and 42-45 days before flowering of step 2), but it is not limited to this.

The watering of step 2) is preferably watered sufficiently into the soil layer 40 to 45 days after seeding the flax seeds, and if there is not enough water in the soil, gibberel chloride biosynthesis inhibitor mepiquat chloride or trinexapak The effect of ethyl is significantly reduced.

The spraying of step 3) is preferably foliar spraying on the flax plant after the flax seed wavelength but before the flax flower blooms on the 50th day, but is not limited thereto.

The mepiquat chloride of step 3) preferably has a concentration of 200 to 600 ppm in an aqueous solution, and more preferably 500 to 600 ppm, but is not limited thereto. When the mepiquat chloride is less than 200 ppm in aqueous solution, it does not effectively reduce the content of gibberellin and thus does not increase the yield of flax seed.

The trinexapac ethyl of step 3) preferably has a concentration of 100 to 300 ppm in an aqueous solution, and more preferably 200 to 300 ppm, but is not limited thereto. When the trinexapak ethyl is less than 100 ppm in an aqueous solution, it does not effectively reduce the content of gibberellin and thus does not increase the yield of flax seed.

The method of increasing flax seed production and oil content of the present invention increases flax seed production and oil content of flax seeds, and since linolenic acid, an unsaturated fatty acid, significantly improves the content of flax seeds, the flax seed production is increased, and the oil content and linolenic acid It may be useful for growing flax seed growing methods.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.

However, the following examples and experimental examples are intended to illustrate the present invention in detail, and the content of the present invention is not limited by the examples and the experimental examples.

< Example  1> Cultivation method to increase seed production and oil content of flax

In order to increase the seed production and oil content of flax, 500 g per 300 pyeong of selected flax seeds (variety name: Hwanamam, Gyeongsangbuk-do Agricultural Research and Extension Services) were lined at 30 cm intervals per week, and the seeding depth in the soil was 1 cm. Use Then sprayer enough 1 liter mepi quart chloride (Mepiquat chloride) 600 ppm solution to 10 m ² per gibberellin biosynthesis inhibitor, the water in the 50 days Dan counter after sowing into the soil 40 to 45 days after planting probably a plant Evenly foliar spray. Seeds of flax were harvested from 80 to 110 days after sowing, and the cultivation method and the harvested seeds were used for the following experiments (FIG. 1).

< Experimental Example  1> Gibberellin  Identification of Seed Growth and Seed Yields of Flax Plants by Application Levels of Biosynthesis Inhibitors

In order to check the growth and seed production of flax plants according to the spraying concentration of mepiquat chloride (MC) or Trinexapac-ethyl, mepiquat chloride and Trinexapak ethyl were prepared by concentration and The seeds were sprayed on plants, and flax seeds were harvested under the same conditions as in Example 1, except that the mepiquat chloride and trinexapak ethyl were sprayed at different concentrations. In addition, the measurement of plant height (cm), number of branches (dog), number of pods (dog), ripening ratio (%) and weight in grains (g) of flax plants were measured by a known method, and then converted to an average value, and then compared. It was.

As a result, as shown in Table 1, in the case of trinexapakethyl compared to the untreated, the height was smaller as the concentration was higher, the branch number was small at a low concentration, it was confirmed that increased at a high concentration. The maturity ratio, which is an indicator of the fidelity of seeds, was significantly increased when compared with no treatment, and the yield of seeds was also increased. On the other hand, mepiquat chloride was found to decrease in height as the concentration was increased and the number of branches increased than Trinexapakethyl. In particular, it was confirmed that the weight of the grains was heavier as the concentration was increased, and the seed yield was the highest in water yield from 600 ppm to 196 kg (Table 1).

Medicine Spray concentration
(ppm) Plant height
(cm) Basin
(dog) Pod
(dog) Ripening rate
(%) In the sky
(g) Seed quantity
(kg / 10a) No treatment 85.6 6.4 17.1 88.6 5.91 161 Mepiquat
Chloride
200 81.4 6.6 18.9 93.7 6.30 179 400 79.3 7.9 20.2 93.5 6.31 186 600 76.5 8.5 21.5 94.1 6.42 196 Trinexa Park
ethyl
100 82.1 6.5 17.2 90.2 5.93 174 200 82.5 6.8 17.5 91.2 6.31 176 300 80.3 8.1 18.7 91.9 6.32 180

< Experimental Example  2> Gibberellin  Determination of Oil Content, Iodine Value and Unsaturation Ratio by Application Levels of Biosynthesis Inhibitors

In order to check the oil content and quality change of flax plants according to the spraying concentration of Mepiquat chloride (MC) or Trinexapac-ethyl, mepiquat chloride in the same manner as in <Experimental Example 1> And the oil content of linseed seed, the iodine value indicating the fat maintenance characteristics, and the unsaturated ratio indicating the oil quality according to the Trinexapak ethyl sparging concentration were measured according to the method described in the Food Code (2001).

As a result, as shown in Table 2, the oil content was 382 g / kg, which was the highest at 600 ppm of mepiquat chloride, and the iodine value, which indicates the fat maintenance characteristic, was 163 mg / g. In particular, the unsaturated ratio indicating the quality and value of the oil was 13.3 at 600 ppm of mepiquat chloride, and it was confirmed that it had the best oil properties (Table 2).

Medicine Spray concentration
(ppm) Oil content
(g / kg) Iodine
(mg / g) Desaturation ratio No treatment 356 155 10.1 Mepiquat
Chloride
200 353 159 11.0 400 359 162 12.5 600 382 163 13.3 Trinexa Park
ethyl
100 359 159 10.9 200 357 161 12.5 300 361 163 14.4

< Experimental Example  3> Gibberellin  Confirmation of the proportion of oil composition according to the application concentration of biosynthesis inhibitor

In order to confirm the oil composition change of flax plants according to the spraying concentration of Mepiquat chloride (MC) or Trinexapac-ethyl, mepiquat chloride and trimethium in the same manner as in <Experimental Example 1> Fatty acid analysis of flax seeds was performed according to nexapak ethyl sparging concentration.

Specifically, fatty acid analysis according to the composition ratio of flax seeds according to the mepiquat chloride and Trinexapak ethyl spraying concentration cultivated in the <Experimental Example 1> after extracting the fat by Soxhlet method and pretreatment with reference to the Food Code It was then analyzed by GC (Agilent GC 7890, Agilent Technologies, Santa Clara, CA, USA) equipped with a capillary column (SP-2560, 100 m × 0.2 μm × 0.25 mm). The inlet and detector (FID) temperatures were set to 260 ° C., and the oven temperature was maintained at 180 ° C. for 40 minutes and then heated to 3 ° C. per minute to 230 ° C. for 20 minutes. Carrier gas was used as helium gas and the split ratio was set to 20: 1. Each fatty acid was quantified by comparing the retention time with standard fatty acids (FAME Mix C4-C24, 100 mg, Neat, Supelco, Bellefonte, PA, USA) under the same conditions.The content indicates the area of each peak as a relative percentage. It was.

As a result, when mepiquat chloride was applied as shown in Table 3 below, as the concentration increased, the saturated fatty acids palmitic acid and stearic acid decreased while the unsaturated fatty acids oleic acid, linoleic acid and linolenic acid increased. Linolenic acid composition ratio of 600 ppm chloride was 61.3%, the highest result was confirmed (Table 3).

Medicine
Spray concentration
(ppm) Composition ratio (% Peak area) Palmitic acid Stearic acid Oleic acid Linoleic acid Linolenic acid No treatment 5.2 3.6 17.7 16.0 55.5 Mepiquat
Chloride
200 5.0 3.2 18.7 16.4 55.6 400 4.6 2.8 19.5 17.0 57.1 600 4.6 2.9 20.5 18.6 61.3 Trinexa Park
ethyl
100 5.2 3.3 9.1 17.0 56.5 200 4.7 3.0 19.7 18.4 59.1 300 4.2 2.5 20.3 18.4 57.6

Claims (9)

delete delete delete delete delete delete 1) sowing flax;
2) sowing the flax seeds of step 1), and then watering the soil after 40 to 45 days before flowering; And
3) after sowing, 50 il mepi quart chloride (Mepiquat chloride) 200 to 600 ppm, or tri-neck sapak ethyl (Trinexapac-ethyl) maybe sprayed on the plant leaf area in 80 ㎖ to 100 ㎖ per 1m ² at 100 to 300 ppm concentration A method for increasing the yield and oil content of flax seeds, the method comprising:

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