KR101262626B1 - An fertilized egg incubation method for takifugu - Google Patents

Abstract

PURPOSE: A river puffer fertilized egg hatching method is provided to utilize a colloid remover for a river puffer fertilized egg in order to safely and easily produce seeds of the river puffer. CONSTITUTION: A river puffer fertilized egg hatching method is composed of the following: a step of artificial fertilization by collecting eggs and sperms from a mother river puffer which is cultured in seawater; a step of washing the artificially fertilized eggs with filtered-sterilized water; a step of removing colloids by stirring the washed fertilized eggs in an fertilized river puffer eggs incubator(4) which is installed with beads after the incubator is aerated by mixing breeding water and acid clay; and a step of hatching the fertilized eggs by accommodating the fertilized eggs in the incubator which is installed with beads to prevent the backflow of the inflow water and the loss of the fertilized eggs when the breeding water does not flow into the inside which is in contact with an inflow pipe(41) of the lower part of a funnel. [Reference numerals] (AA) Pump; (BB) Filtering device; (CC) Sterilizing device

Description

Fertilized egg incubation method for Takifugu.

The present invention relates to a method that can safely and effectively hatch the yellow fertilized egg using the incubator after removing the adherent mucus of the yellow egg fertilized egg in order to prevent the phenomenon in which the yellow egg fertilized eggs are entangled with each other. More specifically, since the yellowing fertilized egg has an adhesive property, when it is housed in the incubator, the inner surface of the incubator and the fertilized egg are entangled with each other, so that the hatching rate is lowered. The present invention relates to a hatching method using decoction of bokbok fertilized egg to control the incubation time by setting the environment such as water temperature in the fertilized egg hatching system for mass production of bokbok seedlings by removing mucus.

Puffer (Takifugu obscurus) is dorsally of acronyms into a fish belonging to the pufferfish (Tetraodontidae) of Tetraodontiformes (Tetraodontiformes) the olive and the stomach is the radiance as a white, x has a wide serotti seonhwangsaek width. The main distribution area is the Yellow Sea, such as the West and South Seas of Korea and the East and South Seas of China, and inhabited by the sea until its motherhood, and from April to May during spawning season, it reaches the upper middle of the river and spawns on the gravel river bed. It is known to have an ecology.

Due to the unique ecological habits of the bokbok, it is known to be caught only in the estuary during the spawning season from April to June, and it is known to lovers only in the spring. Most of the mothers who come to the river for spawning are caught for food production. The amount of resources is rapidly decreasing so that the term is not available.

Unlike other puffer fishes, such as self-propelled or puffer fish, the bokbok is highly adaptable to salt and can be farmed at sea level as well as in domestic water. In addition, since consumption is expected to increase significantly due to the improvement of the diet of people who prefer well-being seafood, it is a fish species that needs to improve productivity and economic efficiency through continuous aquaculture technology development.

Recently, due to environmental pollution and the construction of dams and dams in river estuaries, the spawning grounds of bokbok are disappearing.

On the other hand, at the present research institutes and aquaculture sites, all of them are produced from wild mothers to produce seedlings of bokbok, and the seedling production method dependent on the wild mothers is only possible during April to May, during the spawning season. Since production is dependent on the state of the natural mother, it is impossible to plan seedling production.

All of these seedlings are produced from natural mothers, and the seedling production method that depends on the natural mothers can be produced only during April-May, the spawning season, and the production is dependent on the environment of the water system and the state of the natural mothers. Since it is impossible to produce, it is an obstacle to the development of aquaculture.

In addition, halibut, black sea bream, trout, etc., which are currently the major aquaculture products in Korea, are produced by a professional mother management company to produce fertilized eggs and supply them to seedling producers. Since no method is identified, seedlings are produced using the natural mother. Due to such limitations in technology development, the rate of resource reduction of bokbok is expected to accelerate.

Therefore, in order to prevent the phenomenon in which the incubator inner surface and the fertilized eggs are entangled with each other and the incubation rate is lowered when the fertilized egg is housed in the incubator, the seedling of the bokbok is removed by removing the mucus of the fertilized egg before incubating the fertilized egg in the incubator. It provides a hatching method by removing the colloid of the fertilized egg, which allows the fertilization time to be adjusted by setting the environment such as water temperature or quantity of aquaculture water using the hatching system for producing fertilized eggs.

Korean Unexamined Patent Publication No. 1997-032376 relates to a hatching method of a bokbok, and a method of capturing wild mother bokbok to improve the hatching rate is disclosed, and Korean Patent Publication No. 1997-032375 discloses a method of inducing ovulation In this regard, a composition for identifying appropriate hormones for inducing ovulation of mature males during the spawning season is disclosed. In addition, in relation to aquaculture methods for other fish species, Korean Laid-Open Patent Publication No. 10-2005-0102458 discloses a method for inducing early spawning and seedling production of horsefish, and Korean Patent Publication No. 10-1059515 describes the environment of the reference group. Regarding early spawning induction and cultivation method through water temperature stimulation, Korean Patent Publication No. 10-0872327 discloses early spawning induction and control of seedling production by controlling the maturity of sperm. However, the prior art includes the steps of artificial fertilization by harvesting chaeran from seawater farming mother bokbok; Washing the inseminated fertilized egg with filtered sterilized water; The bottom of the funnel in the shape of a cone shape in the reverse direction is provided with an inlet pipe for inflowing the breeding water, and a part of the upper part is formed with an outlet for discharging the introduced breeding water and is connected to the outflow pipe. If the breeding water does not flow inside the contact with the inlet tube to prevent the backflow of the inflow and loss of the fertilized egg was not disclosed a configuration for the hatching device is installed. In addition, after mixing and aeration of mixed breeding water and acid clay, the fertilized egg is added and stirred to remove the colloid by fertilizing the fertilized egg, and the pump for feeding the fertilized egg and the filtration and sterilizing device for filtration and sterilization in parallel. The water supply pipe connected to the inlet is connected to the warm water tank, and a portion of the inlet pipe connected to the heated water tank is equipped with an inlet water control valve to be connected to the incubator, and the outlet pipe of the incubator is accommodated in the incubator connected to flow to the breeding tank to incubate. The technical features consisting of the steps to make it do not disclose the difference.

The present invention is safe and easy to improve the conventional seedling production method, which requires a skilled technique, a large number of manpower and a large amount of glass plate to modify the egg of the blessed to have a sticky and submerged properties (sedimentary sticking eggs). In order to provide a hatching method using a fertilized egg decoction agent for the production of seedlings.

The conventional method of producing seedlings by attaching fertilized eggs to the glass plate is very difficult to control the adhesion density (1 fertilized egg per 20mm ² ) of the glass plate, which requires many years of skilled technology and a lot of manpower. Was very severe at 30-85%. In addition, when producing a large number of seedlings, a large number of glass plates are required, and due to the area occupied by the glass plates, the fertilized eggs are managed in a large number of breeding areas, which is inefficient due to problems such as raising the breeding water heating ratio.

In order to achieve the above object, the present invention is to provide a safe and easy incubator and hatching method of the bokbok fertilized egg by removing the colloid which is the adhesive component of the bokbok fertilized egg using an incubator. More specifically, the step of artificial fertilization by harvesting chaeran from seawater farming mother bokbok; Washing the inseminated fertilized egg with filtered sterilized water; The bottom of the funnel in the shape of a cone shape in the reverse direction is provided with an inlet pipe for inflowing the breeding water, and a part of the upper part is formed with an outlet for discharging the introduced breeding water and is connected to the outflow pipe. If breeding water does not flow into the interior, the breeding water and acidic clay are mixed and aerated in an incubator with a bead to prevent inflow of backwater and loss of fertilized eggs by blocking the inflow pipe. Thereafter, the fertilized egg is added to the fertilized egg to remove the colloid of the fertilized egg and the pump for feeding the fertilized water and the filtration device and the sterilization device connected in parallel to the water supply pipe connected to the hot water tank, the warm water tank The inflow water control valve is mounted on a part of the inlet pipe connected to the incubator, and the inlet pipe of the incubator is incubated in the incubator connected to the incubator to flow into the breeding tank to incubate. By providing.

The hatching method and hatching apparatus using the colloid removal of the bokbok fertilized egg according to the present invention is the egg (settlement egg) of the property of sinking in the water of fish species other than the bokbok or sticky egg (sedimentary sticking egg) of the property of sinking in water It can be used to contribute to stabilizing seed production of farmed fish. In addition, a large amount of mothers are not required for the production of seedlings, and a large number of tanks are not required for the management of fertilized eggs, which can greatly reduce farming costs, and the incubator capacity is small regardless of the place of incubation due to environmental control in the incubator. It is also easy to control the hatching environment, which can greatly contribute to the industrialization of aquaculture, an expensive aquatic product.

1 is a schematic diagram showing the overall configuration of an incubator and an incubator.
Figure 2 shows a photograph comparing the fertilized egg and the normal fertilized egg according to elimination of the yellowing fertilized egg.
3, Experimental design schematic for identifying proper hatching method of fertilized eggs.
Figure 4 is a photograph showing the experimental method for determining the appropriate hatching method of fertilized fertilized eggs.
Figure 5 shows the experimental design for identifying the proper hatching method of fertilized eggs.
6 is a photograph showing a state in which an oocyte is formed after the mucus is removed using a fertilized egg and an acid clay, and a state in which the eye is formed in the fertilized egg.

The hatching device for removing the colloid of the bokbok fertilized egg according to the present invention is installed in the bottom of the funnel in the shape of the cone shape in the reverse direction, the inlet for breeding water inlet, the outlet portion for the discharge of the breeding water is formed in the upper part When the breeding water does not flow into the inside contacting the inlet pipe of the lower part of the funnel, it is characterized in that the bead is installed to prevent the inflow of backwater and loss of fertilized eggs.

In addition, the hatching device for removing the colloid of the bokbok fertilized egg is a water supply pipe connected in parallel to the pump for breeding water supply, the filtration device and the sterilization device for filtration is connected to the hot water tank, to a part of the inlet pipe connected to the warm water tank The inlet water control valve is mounted and connected to the incubator, the outlet tube of the incubator is characterized in that connected to flow to the breeding water ���.

Another hatching method using a hatching device for removing the colloid of the bokbok fertilized egg of the present invention comprises the steps of artificial fertilization by harvesting the eggs from the seawater farming mother bokbok; Washing the inseminated fertilized egg with filtered sterilized water; The bottom of the funnel in the shape of a cone shape in the reverse direction is provided with an inlet pipe for inflowing the breeding water, and a part of the upper part is formed with an outlet for discharging the introduced breeding water and is connected to the outflow pipe. If the breeding water does not flow into the interior, the breeding water and acidic clay are mixed and aerated in an incubator with a bead to prevent inflow of backwater and loss of fertilized eggs by blocking the inflow pipe. Step to remove the colloid by washing the fertilized egg and the water supply pipe connected in parallel with the pump for feeding the fertilized egg and the filtration device and sterilization device for filtration and connected to the hot water tank, the inlet pipe connected to the hot water tank Inlet flow rate control valve is attached to part of the incubator, the outlet pipe of the incubator is connected to flow to the breeding tank Receiving a screen device characterized in that comprising the step of incubation.

The acidic clay used in the incubation method using the incubation device for removing the colloid of the bokbok fertilization is a particle size of 125-250μm, the stirring time of the fertilized egg mixed with the acidic clay is made for 3-5 minutes, the fertilized egg 60% accommodated in the incubator After the eyeball is formed in the above it is characterized in that the fertilized egg is transferred to the farm and hatched.

1. Incubation method using hatching device and hatching device

1) Incubation device

1 is a schematic diagram showing the overall configuration of an incubator and an incubator. The incubator is an inverted cone-shaped funnel container with a diameter of about 30 cm and a height of 50 cm. The lower end is connected to a tube formed integrally with a hole for inflow of breeding water and has an inner diameter of 10 mm. In addition, the bead is contained inside the container to prevent the inflow of beads in the breeding water to prevent the inflow pipe to prevent the inflow of backflow and loss of fertilized eggs. In order to discharge the breeding water introduced from the lower inlet pipe, a part of the upper part is formed by connecting a discharge pipe, the position of which is 20 mm from the top, and the inner diameter of the drain pipe is about 16 mm, but is not limited thereto. A hose can be connected to this outlet to transfer hatched bokbok to a desired location or to capture large quantities of young bokbok. The hatching vessel is a transparent synthetic resin product that is durable and easy to observe from the outside.

The hatching device including the incubator is composed of a pump for feeding water, a filter (5 μm) for filtration, a UV sterilizer, a warm water tank, and an incubator to facilitate artificial environmental management for safe seedling production. The warm water tank is made of pedestal or support and fixed at higher point than the incubator and breeding tank so that the breeding water flows out by natural water pressure. In addition, it is possible to adjust the water pressure by installing a valve in the heating water tank and the incubator connecting pipe, and by connecting a different valve to the valve at the bottom of the heating breeding tank to connect a plurality of incubators to capture the young bokbok hatched in the desired breeding tank do. Inside the heated water bath, a thermostat of 2 ~ 3KW is installed to regulate the water temperature.

2) Incubation method using incubation device

First, the breeding water is sucked into the pump and passed through the filter to remove solids of 5 μm or more, and then, through the UV sterilizer, the microorganism is sterilized and introduced into the warm water tank, and then the breeding water is transferred to the tank through the incubator. Before the fertilized egg of the bok is incubated in the incubator, the fertilized egg of the fertilized bokbok to remove the mucus in order to prevent the incubation rate is lowered due to the inner surface of the incubator and the eggs entangled with each other.

At this time, the effective decolloid agent is a fuller's earth having a particle size of 125-250 μm, and 10 g of the decollector agent is added to the incubator in a state in which 50% of breeding water is filled and strongly aerated. After that add fertilized egg of the bokbok and leave for 3 minutes. Mixing of eggs and decollector is sufficient if strong aeration. After 3 minutes, the incubator is lifted up, the contents are poured into a 500-μm nylon mesh, and the breeding water is flowed for about 10 minutes to remove the colloid and impurities.

Acidic clay used in the present invention is almost similar to bentonite in appearance, but has more silicic acid content and less alumina components than bentonite. However, the adsorption force is stronger than bentonite, which is acidic. Acidic clays are typically high in magnesia and are produced by the inclusion of opal or colloidal silica in the form of oppulgite- or montmorillonite, a variant similar to acicular or fine cylindrical keratin. It is a clay mineral that is used as a main mineral and can be obtained through experimental drug substance sales stores.

Conditions for hatching of the eggs to control the hatching time of the eggs by controlling the water temperature, salinity concentration of the breeding water or the number of farming water. Breeding water temperature can be controlled by incubation time by adjusting the temperature around 18 ~ 23 ℃. In addition, the salinity concentration can be set around 10 ~ 5 ‰, the quantity is 6 ~ 10ℓ per minute, the appropriate quantity can be adjusted by setting the environment within about 8ℓ per minute to control the incubation time.

When the fertilized egg from which the mucus was removed was incubated in the hatching apparatus of the present invention, the hatching rate reached 94%.

Hereinafter, with reference to the accompanying drawings, each process with respect to the method and hatching device of the bokbok fertilized egg according to the present invention will be described in detail.

2. Determination of the removal method

  1) Secured fertilized eggs

Eggs were collected and harvested from eight mature three-year-old mothers (5 females, 3 males). After artificial insemination, the fertilized eggs were placed in a 500 µm nylon mesh and washed with filtered sterilized water (saline 5 ‰, water temperature 20 ℃) for 5 minutes. It was used for the experiment. The fertilized egg secured was 240,000 and fertilization rate was 98%, which was mixed evenly before use in the experiment.

  2) Experimental Design

In order to identify the proper colloidal determinants of fertilized eggs, whole milk powder and tannins used in other farmed fish were compared with 125'250μm fuller's earth.

Before securing the fertilized egg, four conical vessels (30 cm in diameter and 50 cm in height) were prepared, and 50% of the bred water (salt 5 ‰, water temperature 20 ° C.) was equally filled in the container. Thereafter, airstone (25 cm) was put in each container to allow the breeding water in the container to be sufficiently agitated, and then each powder of milk, white clay, and tannin was added to each of the three containers, and 50,000 eggs were added thereto.

One remaining container was used as a control and only fertilized eggs were added without decolloid. The fertilized egg was placed in a container, stirred for 3 minutes, and immediately moved to a separate nylon net (500 μm). After removing the colloid remover with running water for 5 minutes, the incubation rate was measured with an incubator.

  3) Fertilized egg management and hatching rate

The incubator water temperature of all experimental groups was maintained at 22 ° C. by raising 1 ° C. every day after stocking fertilized eggs at 20 ° C. The specific gravity of the breeding water was maintained at 5 ‰ and the return volume was adjusted to 8ℓ / 1min. After 5 days of fertilization, fertilized eggs were hatched, and hatching volume was determined by capturing hatching larvae in a catching net made of nylon with a diameter of 500 µm. Hatching rate was evaluated by counting the hatched larvae for 5 days immediately after hatching.

  4) Results

As a result of the experiment to identify the proper colloid remover of the fertilized eggs, the hatching rate was 41.2%, whole milk powder 53.6%, tannin 12.7%, and white clay 94.2%. Figure 2 shows a photograph comparing the fertilized egg and the normal fertilized egg according to elimination of the yellowing fertilized egg.

3. Identification of proper hatching method for fertilized eggs

  1) Secured fertilized eggs

Eggs were harvested and harvested from seven mature three-year-old mothers (5 females, 2 males), and after fertilization, the fertilized eggs were placed in a 500 µm nylon mesh and washed with filtered sterilized water (saline 5 ‰, water temperature 20 ℃) for 5 minutes. ) Was used for the experiment. The fertilized egg secured 235,000 grains, and the fertilization rate was 97%, which was evenly mixed before being used in the experiment.

   2) Experimental Design

In order to identify the proper hatching management method, the fertilized eggs were sprayed on the bottom of the tank without removing the colloids, sprayed on the bottom of the tank after removing the colloids, glass plates were attached, and the hatching management using the incubator after the removal of the colloids was compared. 3, Experimental design schematic for identifying proper hatching method of fertilized eggs.

The experimental group sprayed on the bottom of the tank without removing the colloids secured the fertilized eggs and then sprayed 50,000 granules evenly into a 30-ton concrete tank (experimental group 1), and the glass plate attached experimental group attached the fertilized eggs to the glass plate of 40 × 60 cm. Incubation rate evaluation (experimental group 2) was performed. At this time, 20,000 grains of fertilized eggs were attached per glass plate to lower the normal adhesion density. Experiment 3 prepared a conical container (30cm in diameter and 50cm in height) before securing the fertilized egg, and filled the breeding water 50% in the container, and then put the air stone (25cm) in the container so that the breeding water was sufficiently stirred, and then added 10g of acidic clay. Fertilized eggs were inserted 50,000.

The fertilized egg was placed in a container and stirred for 3 minutes, and then the contents of the container were immediately transferred to a nylon net (500 μm), and the colloid remover was removed for 5 minutes with running water, and then sprayed evenly on the bottom in a 30 ton water tank (Experimental Group 3). Evaluation of hatching rate using incubator after removing colloid The experimental group carried out hatching rate measurement by carrying out fertilized eggs in a conical incubator after removing colloid and washing in the same manner as Experimental group 3 (Experimental Group 4).

After receiving the fertilized eggs for each method, the experimental groups 1, 2, and 3 were adjusted to have flow rate of 15 l per minute and the experimental group 4 to 8 l per minute. Breeding water of all experimental groups was adjusted to a water temperature of 21 ℃, 5 ‰ salt.

Fertilized eggs hatched from 5 days after fertilization in all experimental groups. Experimental groups 1, 2, and 3 were collected in 1 liter containers for 15 times each night and converted to 30 tons after counting. Hatching larvae were trapped in a catch network made of 500 ㎛ nylon net in the incubator discharge pipe to determine the hatching quantity. Hatching rate was evaluated by counting the hatched larvae for 5 days immediately after hatching. Figure 4 is a photograph showing the experimental method for identifying the yellowing fertilized egg titration hatching method.

  3) Results

As a result of experiment to investigate the proper hatching management method, the hatching rate of each experimental group was 33.2% in Experiment 1, 56.7% in Experiment 2, 41.6% in Experiment 3, and 93.4% in Experiment 4, and the fertilized egg of the Bok was incubated after removing the colloid. Hatching and management were found to be desirable.

4. Identification of fertilized egg treatment method for improving hatching rate of fertilized eggs

  1) Secured fertilized eggs

Eggs were collected and harvested from six mature three-year-old mothers (females 4 and males 2). After fertilization, the fertilized eggs were placed in a 500 µm nylon mesh and washed with filtered sterilized water (5 salts, water temperature 20 ° C) for 5 minutes. ) Was used for the experiment. The fertilized eggs were 140,000 and fertilization rate was 97%.

  2) experimental design

Figure 5 shows the experimental design for identifying the proper hatching method of fertilized eggs. In order to identify the proper transportation time and method of fertilized eggs, the embryos have been removed from the embryos for 3 hours, and embryo fertilization method (bottom spraying and incubator) after 3 hours, and fertilized eggs are transported (after fertilization and at least 60% According to the experiment was designed by a total of six experimental groups (Fig. 5).

Each experimental group evaluated hatching rate using 20,000 fertilized eggs. Experimental group 3, 4, 5 and 6 decolloid was mixed with fertilized eggs using 125-250μm acidic clay (fuller's earth) and stirred for 3 minutes and then washed.

The transfer method of fertilized eggs in consideration of the transfer from the seedling production plant to the farm is a common transportation method, in which 10 liters of breeding water (saline 5 ‰, water temperature 20 ℃) is put in a 20ℓ transparent plastic bag, and the fertilized eggs are filled and oxygen is charged. Sealed and transported. Fertilized egg transport was carried out using a passenger car loaded with a plastic bag containing the fertilized egg 3 hours after moving the fertilized egg was determined according to the design of each experimental group hatching rate. When the fertilized egg is loaded into the vehicle, the internal temperature of the vehicle is maintained at 20 ° C.

After transporting the fertilized eggs, the floor spraying test group (experimental groups 1, 3 and 5) was adjusted to keep the return water in a two-ton circular plastic water tank once a day, and the experimental group using the incubator (experimental groups 2, 4 and 6) was accommodated in the incubator. After that, the amount of return was maintained at 10 L per minute. Breeding water of all experimental groups was maintained at 5 ‰ salt, water temperature 20 ℃. FIG. 6 is a photograph showing a state in which a mucus is removed using a fertilized egg and a fuller's earth, and then a state is formed in the fertilized egg after the mucus is removed.

  3) Experiment result

Table 3 shows the experimental results to determine the proper transportation time and method of fertilized eggs.

Appropriate hatching method of fertilized eggs division Experiment 1 Experiment 2 Experiment group 3 Experiment group 4 Experiment group 5 Experiment group 6 Hatching rate 5.6% 9.3% 12.9% 37.7% 40.7% 84.6%

As a result of the above experiment, proper fertilization time for fertilized eggs in fertilized eggs was improved after hatching in 60% of fertilized eggs and hatching rate was improved. Shipping by packaging has been shown to be the preferred method. When transported to the incubator after transportation, the hatching rate reached 84.6%.

Seedling of bokbok by using hatching device for decoction of bokbok fertilized egg of the present invention in the method for producing seedling of bokbok, which requires skilled manpower and a lot of manpower during the spawning season, April-May, depending on the natural mother bokbok in a common farming field It does not require a lot of mothers for production, and does not need a large number of tanks for the management of fertilized eggs, which can significantly reduce aquaculture costs. It can be greatly contributed to the industrialization of the cultivated bokbok, which is an expensive aquatic product, and can be developed into an effective cultivation industry of the bokbok.

1: water distribution pipe 2: heated water tank support
3: inflow control valve 4: incubator body
5: breeding tank 6: discharge pipe
7: incubator stand 8: hot water bath
41: inlet tube 42: backflow prevention beads
43: outlet pipe

Claims (5)

a) artificial fertilization by harvesting eggs from seawater farming mothers;
b) washing the fertilized fertilized egg with filtration sterilized water;
c) funnel of the cone shape in the reverse direction of the funnel, the bottom of the funnel is installed inlet for breeding water inlet, a portion of the top is formed with an outlet hole for the discharge of the inflowing breeding water is connected to the outlet pipe and the bottom of the funnel If breeding water does not flow inside the inlet tube, the agglutination of the bokbok fertilized egg incubator with a bead, which prevents the inflow of backwater and loss of fertilized egg, prevents the inflow of the influent and mixes it with aeration. Inserting the fertilized egg and stirring to wash the fertilized egg to remove the colloid;
d) funnel in the form of a separate conical eggs in the reverse direction of the funnel, the bottom of the funnel is installed inlet for breeding water inlet, the upper part of the outflow hole for the discharge of the breeding water is formed, the outflow If the breeding water does not flow into the inside of the funnel connected to the lower part of the funnel, the incubation step is to be accommodated in the incubator with a bead to prevent the inflow of backwater and loss of the fertilized egg. Incubation method using fertilized egg incubator
The method of claim 1, wherein the acidic clay has a particle size of 125-250μm, and the stirring time of the fertilized egg mixed with the acidic clay is 3-5 minutes.
The method of hatching using the bokbok fertilization incubator, characterized in that the fertilized egg is transferred to the farm after hatching is formed in 60% or more of the fertilized egg housed in the incubator after step d) delete delete

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