KR101244711B1 - Composition for the Protection and Treatment of Macular Degeneration Comprising Soybean Sprout Extract - Google Patents

Abstract

The present invention relates to a composition for treating and preventing macular degeneration including soybean sprout extract, and more particularly, it is confirmed that the presence of a large amount of lutein known to have an inhibitory and therapeutic effect on macular degeneration in bean sprouts, and to prevent macular degeneration and It is intended to be used for treatment.
According to the present invention, it is possible to provide bean sprouts as a source of new lutein applicable for the prevention and treatment of macular degeneration.

Description

Composition for the Protection and Treatment of Macular Degeneration Comprising Soybean Sprout Extract}

The present invention relates to a composition for the prevention and treatment of macular degeneration containing soybean sprout extract containing lutein as an active ingredient.

The macula lutea is the part that receives the most clear and accurate light due to the densely packed cells in the retina of the eye, and the disease that causes degeneration of the macular degeneration due to various causes is called macular degeneration. do.

The macular degeneration is one of the three major causes of blindness together with glaucoma and diabetic retinopathy. Age is the leading cause of macular degeneration, and other causes of family history, race, and smoking are known to cause macular degeneration, and damage to the macula causes the eye to lose the ability to see details such as small prints, facial features, or small objects. Done.

There are two types of macular degeneration, non-exudative (dry) macular degeneration and exudative (wet) macular degeneration, 90% of people with macular degeneration have a dry form of symptoms. In dry macular degeneration, waste products form a yellow precipitate called drusen and can accumulate in the tissue under the macula. The presence of drusen interferes with blood flow to the retina, especially the macula and, when blood flow is reduced, reduces nutrient supply to the macula, stopping or contracting the efficient action of photosensitive cells. In wet macular degeneration, new, weak blood vessels can grow in or below the retina, causing fluid and blood to leak into the space below the macula. Wet macular degeneration is sometimes described as choroidal neovascularization, where the choroid is the region of the blood vessels under the retina and angiogenesis is the growth of new blood vessels in the tissue, as inferred by the name choroidal neovascularization. The blood vessels are born and grow.

Although macular degeneration is considered to be a disease that occurs well in the elderly, it is known that the number of patients in their 4's and 50's has recently increased rapidly. As a major cause of the lowered age group of macular degeneration, westernization of eating habits such as increased fat intake has been pointed out.

Lutein (lutein) is a naturally occurring carotenoid (carotenoid) that does not have vitamin A activity and is present in large quantities in green yellow vegetables. Lutein, one of the most carotenoids present in food and human blood, is known to play an important role in the body's antioxidant effects and to protect plants from UV rays. It is known to play an important role in health and vision enhancement.

According to US Department of Agriculture data, the average daily lutein intake of Americans is currently about 1.7 mg and Europeans are about 2.2 mg, which is the risk of eye diseases such as cataracts and age-related Macular Degeneration (AMD). Has been reported to reduce levels, and the recent increase in the use of lutein in dietary supplements and certain food and beverage ingredients can help increase lutein intake to more effective levels in the US and Europe. It has been reported.

Lutein is known as a major component of green vegetables and fruits such as broccoli, green beans, mallow beans, cabbage, kale, spinach and kiwi, but the green vegetables and fruits include lutein, beta carotene and carotenoid epoxy compounds in addition to lutein. Since the coexistence and purification of lutein from them is a problem that requires a high cost and a complicated procedure, in particular, it is often present as a lutein ester compound having a relatively lower physiological activity than free lutein.

Lutein is also abundant in many yellow / orange fruits and vegetables, such as mangoes, papayas, peaches, plums, acorns, pumpkins, zucchini, and oranges, but the lutein contained in these yellow / orange fruits and vegetables may be It is present in esterified form with organic acids or fatty acids such as myristic acid, lauric acid and palmitic acid. This is also relatively lower in physiological activity than free lutein because they have an inefficiency that lutein esters must be hydrolyzed and regenerated into free lutein when consumed in food and then absorbed and metabolized in the body. Therefore, in order to effectively use the lutein contained in these yellow / orange fruits and vegetables, it is necessary to separate and purify the glass after saponification, which induces relatively high cost and time consumption. Carotenoids also contain high concentrations, resulting in low relative purity.

To date, the treatment for macular degeneration and improvement is mostly the use of marigold extract. Marigold petals are considered good sources of lutein because they contain significant amounts of lutein and not much other carotenoids.

However, since lutein present in marigold does not exist as free lutein but in the form of a lutein ester compound, marigold extract is also used by converting lutein fatty acid ester to lutein through saponification, and saponification reaction. Nevertheless, there is a problem that the lutein produced is still contaminated with some chemical impurities.

Meanwhile, soybeans ( Glycine max L.) is Korea's main food crop, which contains a large amount of protein (about 40%) and lipids (about 20%), and is an excellent food in terms of nutrition. It is also an important source of vegetable oil. Soybeans are widely used as foods such as fermented products (soybean paste, cheonggukjang, red pepper paste, soy sauce, etc.), germinated products (soybean sprouts), and other processed products (soy milk, tofu, cooking oil, etc.), and also for industrial use (medicines, cosmetics, soap, etc.). It is also widely used as a raw material.

Recently, various researches on soybean quality have been actively conducted, and the characteristics for each use have been revealed.

Bean sprouts means the state of bean sprouting. Until now, technology development related to such bean sprouts has been mainly made in the field of bean sprout cultivation device, and improved method of growing bean sprouts or developing methods for growing bean sprouts with enhanced specific nutritional ingredients. The back was mostly.

In other words, it has not been found that soy sprouts contain large amounts of lutein.

The inventors of the present invention confirmed that lutein is contained in bean sprouts during the search for new plant resources having a therapeutic and preventive effect on macular degeneration, and completed the present invention.

Therefore, an object of the present invention is to provide a pharmaceutical composition for the treatment and prevention of macular degeneration containing soybean sprout extract as an active ingredient.

In addition, the present invention is to provide a health food for improving and preventing macular degeneration containing soybean sprout powder or bean sprout extract as an active ingredient.

As an example for achieving the above object, the present invention is characterized by a pharmaceutical composition for treating and preventing macular degeneration comprising a bean sprout extract.

The bean sprout extract is characterized by containing the lutein represented by the following formula (1) as an active ingredient.

As another example for achieving the above object, the present invention is characterized by improving the macular degeneration and preventive health food comprising soybean sprout powder or bean sprout extract.

Hereinafter, the composition for treating and preventing macular degeneration including the bean sprout extract of the present invention will be described in detail.

Firstly, “macular degeneration” as used in the specification and claims of the present invention should be interpreted to include all forms of macular degeneration disease, regardless of the age of the patient, although some macular degeneration diseases are more common in certain age groups. will be. The causes of macular degeneration include, but are not limited to, hereditary, physical trauma, diabetes, diseases such as malnutrition, bacterial infections, and the like.

The method of preparing the bean sprout extract of the present invention is as follows.

First, the sprouts composed of cotyledons and hypocotyls are germinated to dry, pulverized to make a powder, and the filtrate obtained by immersing them in acetone and filtering is concentrated under reduced pressure to obtain acetone extract from which acetone is removed.

Sprouts are sprouted soybeans, including cotyledons and hypocotyls, soybean sprouts can be used from the time when the cotyledons and hypocotyls are separated after germination, preferably using sprouts 2 to 20 days after germination. It is preferable in terms of obtaining lutein.

Although there are some differences depending on the varieties of beans, lutein is contained in the range of 2 to 60 ㎍ in 1g of dried bean sprouts, more than 80% of the cotyledons among the whole bean sprouts, and lutein contained in the cotyledons usually 2 to 100 Μg / g level. Therefore, in the present invention can be used together with the cotyledon or hypocotyl of the bean sprouts or cotyledon and hypocotyl, it will be possible to obtain lutein with high efficiency when used separately.

The bean sprouts may be grown by the conventional bean sprout cultivation method, soybean seeds obtained by immersing the seeds in water and sprinkled by intermittent spraying in a dark state in which room temperature and humidity are maintained.

Lutein has 40 carbons and 56 hydrogens, but it shows fat solubility due to its structural features including only a few hydroxyl groups (OH). Therefore, it is not soluble in a completely polar solvent such as water. Therefore, it is preferable to use a polar or nonpolar organic solvent as an extraction solvent.

As the polar or non-polar organic solvent, lower alcohols having 2 to 5 carbon atoms, acetone, acetonitrile, ethyl acetate, chloroform, dichloromethane, ethyl ether, xylene, hexane and the like may be used.

As the organic solvent, the polar solvent having a polarity of 5 to 6, such as alcohol or acetonitrile, is relatively well soluble. However, these solvents have excellent solubility in various polar substances including sugars. When organic solvents are used, there is a tendency to comprehensively extract lutein and other polar substances including sugars, flavonoids, glycosides, and proteins.

In addition, lutein, which shows fat-soluble properties, can be extracted well using organic solvents such as ethyl acetate, chloroform, butanol, propanol, dichloromethane, ethyl ether, xylene, and hexane, which exhibit relatively moderate or non-polar characteristics. The solubility of substances with lower polarity than lutein, such as chlorophyll compounds with lower polarity, pheophytin derivatives, carotene compounds including alpha and beta carotene, lipids, and the like, is extremely increased, leading to greater incorporation of nonpolar substances.

On the other hand, acetone shows a level of 5.1 similar to alcohols in terms of polarity, but the sugar, glycoside, and protein components present in a large amount in the plant are extremely poor in solubility in acetone, and thus acetone is not easily extracted when used as a solvent. Even if a small amount is extracted, cold soaking has the advantage that the solubility is extremely low and precipitates in the form of a precipitate, which can be removed by filtration, thereby extracting lutein of relatively high purity.

The solvent may be used in a weight of 5 to 20 times the weight of the bean sprout powder, the extraction may be applied to a variety of methods, such as low temperature dark conditions extraction, room temperature extraction, ultrasonic extraction.

The bean sprouts extract from which the acetone was removed was concentrated, and then column chromatography was performed to obtain fractions of lutein, and each fraction was concentrated to dryness and redissolved, followed by pure separation using preparative HPLC.

The structure of the obtained lutein was identified by various spectroscopic methods such as NMR, MS, etc. As a result, lutein having the structure shown in Chemical Formula 1 was obtained, which is a known substance.

The lutein plays an important role in protecting the plant from ultraviolet rays and antioxidant effects in the plant body, and is widely known to humans as a major component of the macular and lens of the eye to enhance eye health and vision. In addition, the macular pigment density is affected by the concentration of lutein in the serum according to the intake of lutein, and if the macular pigment density is increased by the intake of lutein, the visual acuity is improved, especially in the state of macular degeneration. Increasing the content of lutein as a diet has been reported to stimulate the production of macular pigment may inhibit the progression of macular degeneration.

Meanwhile, in the present invention, the fact that the lutein is contained in the bean sprouts is newly confirmed. In particular, it was confirmed that the lutein contained in the bean sprouts is present in the free form which is active in the body. This is simply obtained by extracting the active lutein in the form of free lutein, which immediately shows lutein activity in the body, by using a solvent, in order to extract lutein existing in the ester salt state from the existing marigold extract. The advantage is that you can do it.

Therefore, the bean sprouts may be used as a raw material of the composition for the treatment, prevention and improvement of macular degeneration.

Macular degeneration treatment and prevention composition comprising the bean sprout extract or lutein of Formula 1 as an active ingredient is administered orally or parenterally during clinical administration, for example, intravenous and arterial, intramuscular, subcutaneous, intraperitoneal, mucosal or It can be applied topically (eg, eye drops), eye, transdermally, etc.

The compositions may be formulated for oral administration such as tablets, troches, lozenges, aqueous or oily suspensions, prepared powders or granules, emulsions, hard or soft capsules, syrups or elixirs. And the like. Binders such as lactose, saccharose, sorbitol, mannitol, starch, amylopectin, cellulose or gelatin for formulation into tablets and capsules; Excipients such as dicalcium phosphate; Disintegrants such as corn starch or sweet potato starch; Lubricants such as magnesium stearate, calcium stearate, sodium stearyl fumarate or polyethylene glycol wax. In the case of a capsule formulation, in addition to the above-mentioned substances, a liquid carrier such as fatty oil is contained.

In addition, the composition of the present invention can be administered parenterally, and parenteral administration is applied to subcutaneous injection, intravenous injection, intramuscular injection, intrathoracic injection and mucosal or topical, dispersant, suppository, powder, aerosol ( Nasal sprays or inhalants), eye drops, gels, suspensions (aqueous or non-aqueous liquid suspensions, oil-in-water emulsions or water-in-oil emulsions), liquid dosage forms suitable for parenteral administration, such as solutions. To formulate into a parenteral formulation, the composition is mixed in water with a stabilizer or buffer to prepare a solution, which may be formulated in unit dosage forms of ampoules or vials.

The effective dosage of the bean sprout extract of the present invention or the lutein of Formula 1 may be varied according to the age, physical condition, weight, etc. of the patient, but is generally 1 to 20 mg / day per kg of adult patient weight, preferably 5-10 mg / day, and may be divided into several times a day, preferably two to three times a day at regular time intervals, depending on the judgment of the doctor or pharmacist.

In addition, the bean sprout extract of the present invention or lutein represented by the formula (1) has been identified and separated from the edible bean sprouts are safe for human body is suitable for use in addition to food.

On the other hand, bean sprouts may be used as a raw material of health foods having the effect of improving and preventing macular degeneration, wherein the bean sprouts may be used in the form of bean sprout powder, bean sprout extract.

When the bean sprout powder or bean sprout extract is included in the health food, it may be used in the range of 0.01 to 50% (w / w), preferably 1 to 30% (w / w) of the total weight.

That is, various foods can be prepared by conventionally known methods by adding them to conventional palates such as noodles, tofu, cereals, breads, chewing gum, candy, confectionary, etc., and as edible food additives. It may be applied, and may also be formulated into a general formulation such as tablets, granules, pills, hard capsules, soft capsules, or liquid formulations, and may be prepared in fresh juice, pouches, beverages, or teas, The components may be appropriately selected and blended by those skilled in the art according to the dosage form.

According to the present invention described above, the effect that can be used as a source of new lutein sprouts soybean sprouts.

In addition, according to the present invention, in addition to using soybean as a seed nutritional state as a main nutritional function, it is confirmed that the germinated bean sprouts state contains a variety of bioactive substances beneficial to the human body, and thus can be utilized for more diverse uses of soybeans. You can expect an effect that can be harmonized.

1 is a photograph of bean sprouts germinated.
Figure 2 shows the HPLC analysis chromatogram of bean sprout extract.
Figure 3 shows the ¹ H-NMR spectrum of the lutein compound isolated from the bean sprout extract.
Figure 4 shows the mass spectrometry spectrum of the lutein compound isolated from the bean sprouts extract.
5 is a UV-VIS of the lutein compound isolated from the bean sprouts extract. The spectrum is shown.

Hereinafter, the present invention will be described in detail with reference to examples, but the present invention is not limited to the following examples.

Example  1. Cultivation of bean sprouts

Bean sprouts were soaked in plastic bean sprouts cultivation container for 4 hours at 20 ° C. water, and then cultivated at 20 ° C. and 80% humidity. During the cultivation of sprouts, the sprouts were cultivated by sprinkling water for 3 minutes every 4 hours using an automatic watering device equipped with a timer.

Example  2. Preparation of Bean Sprout Extract

500 g of Pungsan Sprout Beans as a sample was cultivated as bean sprouts for 10 days, and the grown bean sprouts were placed in a freezer at -80 ° C., frozen, lyophilized, pulverized and passed through a 60 mesh sieve. Extracted using the passed bean sprout grinding sample.

That is, after adding 1 L of acetone to 100 g of the crushed sample was extracted for 30 minutes in an ultrasonic extractor at 40 ℃ condition, it was extracted three times in the same manner. All the extracted solutions were combined in Whatman No. Filtration was carried out using 2 spots, and the insoluble precipitate was refiltered while standing at -20 ° C low temperature for 24 hours.

The filtered acetone extract was concentrated in a vacuum concentrator at 40 ℃ to prepare a yellow bean sprout acetone extract from which the acetone solvent was removed.

Example  3. Isolation and Structure Identification of Lutein

Some of the bean sprout extracts obtained in Example 2 were dissolved in a small amount of acetone, filtered through a 0.45 μm syringe filter, and analyzed by HPLC. HPLC analysis conditions are shown in Table 1 below, and HPLC analysis results are shown in FIG. 2. Shown in

In addition, separation was carried out using preparative HPLC (Agillent 1200 series, USA) for the bean sprout extract, the column was separated by preparative HPLC, HiQ sil C18-10 (21.0 × 250mm, KYA TECH, Japan) Analytical wavelength 430 nm, flow rate 10 mL / min, solvent A for solvent 75% methanol, solvent B for ethylacetate gradient (0 min: 70% A, 25 min: 15% A, 26 min: 70%) A, 35 minutes: 70% A), and the sample injection amount was adjusted to 2mL to perform high purity separation. As a result of the separation, several kinds of compounds showing yellow and green color were separated, and lutein-containing compounds were analyzed by NMR, UV-VIS spectral analysis and mass spectrometry to analyze the complete chemical structure. Results of chemical shift analysis of lutein's ¹ H-NMR (300 MHz, CDCl ₃ ) results are shown in Table 2, and the ¹ H-NMR spectrum is shown in FIG. 3.

division Condition   column YMC AM 303 (250 × 4.6mm)   Mobile phase (farming condition)     A: 75% MeOH, B: 100% EtOAc   wavelength 430 nm   Flow rate 1.0 mL   Dose 20 μL   Temperature 30 ℃

^{In the 1} H-NMR (300 MHz, CDCl ₃ ) spectral measurement results, when we look at the ¹ H- ¹ H correlation, Me-18 '(1.63 ppm) and H-3' (4.25 ppm) of the alicyclic compound region Presuming to correlate with anterior H-2 '(eq, 1.86 ppm and ax, 1.38 ppm) protons, a peak of 5.55 ppm could be determined as H-4' and H-7 '(5.44 ppm) H-8 '(6.15ppm) and ^{H-6' (2.42ppm) 1} H- 1 H -related signals of the peak could be identified by the (correlation signal). In addition, protons of two secondary hydroxy groups corresponding to H-3 and H-3 'were found in the 4.00 ppm and 4.25 ppm regions, respectively, and H-16 / H-2ax by 1D selective NOESY. viewed as a corresponding to the H-16 / H-17, H-18 / H-17 peak indicating the correlation _{CH 3 -16, CH 3 -18,} H-2ax, H-3 are both present in the same ring I could see. The NOESY correlations of the H-19 / H-7, H-19 / H-10 and H-20 / H-14 peaks were also able to identify CH ₃ bound to the hydrocarbon group. 19 'and H-20' were identified. The proton peak of the olefin group was found to be between 5 and 7 ppm. In particular, the multiplet signal up to 6.65 ppm was H-15 / H-15 '(6.65 ppm) and H-11 / H-. 11 '(6.63ppm), H-7 / H-7' (6.44ppm), H-12 / H-12 '(6.36ppm), H-14 / H-14' (6.25ppm), H-8 / Peaks were identified by pairs of H-8 ′ (6.12 ppm) protons.

Position 隆_H Position 隆_H One One'    2 ax  1.48 (m)   2' ax  1.38 (dd, 13, 7) eq  1.79 (m) eq  1.86 (m) 3  4.00 (m) 3 '  4.25 (m, br)    4 ax  2.05 (m) 4'  5.55 (m, br) eq  2.38 (m) 5 5 ' 6 6 '  2.42 (d, 9.5) 7 6.12 ov ^a 7 '  5.44 (dd, 15.5, 10.1) 8 6.12 ov ^a 8' 6.15 ov ^a 9 9 ' 10 6.17 ov ^a 10 ' 6.14 ov ^a 11  6.63 (dd, 14.3, 11.4) 11 '  6.61 (dd, 15.0, 11.4) 12  6.36 (d, 14.7) 12 '  6.36 (d, 14.7) 13 13 ' 14  6.25 (m) 14 '  6.25 (m) 15  6.65 (m) 15 '  6.65 (m) 16  1.07 (s) 16 '  1.00 (s) 17  1.07 (s) 17 '  0.85 (s) 18  1.74 (s) 18 '  1.63 (s) 19  1.97 (s) 19 '  1.91 (s) 20  1.97 (s) 20 '  1.97 (s)

On the other hand, protons of H-2 (1.48ppm and 1.79ppm) and H-2 '(1.38ppm and 1.86ppm) were identified in different regions from protons of other olefin groups. Instead of pairing with the protons of the group, it was found in the vicinity of 1.3 to 1.8 ppm because of the pairing with the surrounding methyl group. Other methyl groups (CH ₃ , H-16-H-20, H-16'-H-20 ') were identified in the vicinity of 1-2 ppm, a region in which typical methyl peaks appear.

Mass spectrometry of the isolated material [FIG. 4] and UV-VIS. From the analysis of the spectrum [Fig. 5], m / z = 569.3, UV-VIS. Absorption spectra showed λ (nm) = 426, 448, 476.

At least ¹ H-NMR, MS and UV-VIS. As a result of comprehensive analysis of the spectrum, the separation material was yellow, and was identified as (all-trans) -lutein having the formula C ₄₀ H ₅₆ O ₂ = 569.3, which is a known material.

[Formula 1]

Example  4. Confirmation of Lutein Content by Soybean Sprouts

Pungsan Sprout Beans, which are widely used as bean sprouts, are used as raw materials, and after cultivating the bean sprouts for 10 days by the method of Example 1, the lutein content was measured. In particular, when only cotyledons were selected, 81.71 ㎍ / g, and when only the hypocotyls were selected, 2.12 ㎍ / g was included.

As a result, although some lutein is contained in the lower hypocotyl part of the bean sprouts, it can be confirmed that a significant portion of the lutein is contained in the cotyledon part of the bean sprouts.

Therefore, the above results suggest that soybean sprouts, in particular, the cotyledons of soybean sprouts, are considered to have a great applicability as a source of new lutein. In particular, since cotyledons have extremely higher lutein content than lower hypocotyls, Selective use of the bay would be advantageous.

Formulation example .

1) Preparation of Syrup

Syrup containing soybean sprout extract according to the present invention as an active ingredient was prepared by the following method.

The bean sprout extract (2% by weight), saccharin, sugar was dissolved in 80 g of warm water, cooled, and prepared and mixed with a solution consisting of glycerin, sweetener, flavoring agent, ethanol, sorbic acid and distilled water, and water was added thereto. Add to 100 mL.

2) Preparation of Tablets

Tablets containing soybean sprout extract according to the present invention as an active ingredient were prepared by the following method [active ingredient 15 mg].

250 g of the bean sprout extract was mixed with 175.9 g of lactose, 180 g of potato starch, and 32 g of colloidal silicic acid, to which 10% gelatin solution was added, followed by grinding to pass through a 14 mesh sieve. It was dried and tablets were prepared from a mixture obtained by adding 160 g of potato starch, 50 g of talc and 5 g of magnesium stearate.

3) manufacture of beverages

After dissolving 500 mg of the bean sprout extract in an appropriate amount of water, an appropriate amount of vitamin C, citric acid, sodium citrate and oligosaccharides were added, and an appropriate amount of sodium benzoate was added as a preservative to make 100 ml by adding water to prepare a beverage composition. .

4) Manufacture of dietary supplements

Health functional food containing soybean sprout powder according to the present invention as an active ingredient was prepared by the following method.

Bean sprouts powder 1000 mg, vitamin mixture amount, vitamin A acetate 70 μg, vitamin E 1.0 mg, vitamin B1 0.13 mg, vitamin B2 0.15 mg, vitamin B6 0.5 mg, vitamin B12 0.2 μg, vitamin C 10 mg, biotin 10 μg, nicotinic acid Amide 1.7 mg, Folic acid 50 μg, Pantothenate 0.5 mg, Mineral mixture appropriate amount, Ferrous sulfate 1.75 mg, Zinc oxide 0.82 mg, Magnesium carbonate 25.3 mg, Potassium phosphate 15 mg, Dibasic calcium phosphate 55 mg, Potassium citrate 90 mg, calcium carbonate 100 mg, and magnesium chloride 24.8 mg were prepared by mixing according to a conventional health food preparation method.

The present invention described above is not limited to the above-described embodiment and the accompanying drawings, and various substitutions, modifications, and changes are possible within the scope without departing from the technical spirit of the present invention. It will be evident to those who have knowledge of.

In the figures Chl means chlorophyll.

Claims (8)

The bean sprouts were dried and pulverized, soaked in acetone, and the filtrate was filtered at low temperature to remove insoluble precipitates, and the acetone extracts from which the insoluble precipitates were removed were concentrated under reduced pressure to remove acetone, which is effective for treating and preventing macular degeneration. Preparation method of bean sprout extract containing lutein represented by the formula (1) as an active ingredient:
[Formula 1]

delete delete The method according to claim 1,
The bean sprouts used in the bean sprouts extract is a method of producing the bean sprouts extract, characterized in that the cotyledon, hypocotyl, or cotyledon and hypocotyl of the bean sprouts grown in the dark state.
The method according to claim 1,
The bean sprouts is a method for producing soybean sprout extract, characterized in that the cultivated as sprouts up to 20 days after germination.
The method according to claim 1,
The bean sprout extract is a method of producing a bean sprout extract, characterized in that it comprises lutein in the range of 2 to 100 ㎍ / g.

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