KR101218830B1 - composition of brine mineral water suppressing atopic dermatitis - Google Patents

Abstract

The present invention relates to a marine mineral water composition that suppresses atopic dermatitis, and more particularly, to a marine mineral water composition which improves efficacy by deriving a component ratio of an marine mineral water composition that suppresses atopic dermatitis.
Marine mineral water composition according to the present invention contains abundant natural minerals and rare minerals to supply nutrients to the skin, in particular, has the effect of inhibiting atopic dermatitis, and the weakly acidic composition to protect the natural sebaceous membrane It protects the skin from bacteria and improves moisturizing ability to prevent drying of the skin surface.

Description

Composition of brine mineral water suppressing atopic dermatitis

The present invention relates to a marine mineral water composition that suppresses atopic dermatitis, and more particularly, to a marine mineral water composition which improves efficacy by deriving a component ratio of an marine mineral water composition that suppresses atopic dermatitis.

Deep sea water generally refers to ocean water obtained from the ocean of 200m or more. It is characterized by low temperature, rich in inorganic nutrients and trace elements, and has been studied for its basic and practical use for a long time. Recognizing its value as a useful resource, the company is expanding industrialization by extracting useful materials, drinking water and medicine.

As mentioned above, the marine mineral water, which is one of the underground brine, is attracting attention as a saltwater resource along with the deep seawater where research activities and commercialization are steadily being performed.

Marine mineral water, also known as deep rock water, is generally effluent from more than 800 m underground within about 1 km of the coast. Seawater in deep ocean waters is formed along the pores or cracks of the rock in coastal or deep underground rock layers. For example, natural rock groundwater that has flowed down the deep underground aquifers of land and interactions, storage, maturation, and ionization in geological time and space conditions.

Marine mineral waters are affected by the surrounding lipids, and depending on the characteristics of underground soil and rock, not only the composition of water quality but also the useful value can be changed. ), Zinc (Zn), iron (Fe), copper (Cu), nickel (Ni), vanadium (V), selenium (Se) and other minerals, as well as calcium (Ca) and magnesium (Mg) minerals It is reported to academics that the balance is similar to human body fluids.

In addition, it contains a lot of inorganic nutrients such as nitrogen, phosphorus, and silicic acid, almost no contamination by E. coli or general bacteria, and contains a balanced amount of antioxidants, essential trace elements, and various minerals. Indicates.

However, research on the marine mineral water containing abundant human useful components and development of the use thereof are very inferior to the deep ocean water, and currently, the marine mineral water is ingested and consumed as a beverage. .

Korean Patent Publication No. 0543574 discloses a mineral composition and a mineral water including the composition. According to the publication, a mineral composition is extracted from underground deep brine, that is, marine mineral water, and added to purified water to prepare a beverage. When the drink is ingested, useful minerals contained in the subterranean saline solution are supposed to exhibit a good effect on the human body.

In addition, Japanese Laid-Open Patent Publication No. 2001-190256 discloses a mineral water beverage containing mineral components derived from deep water, and water-soluble mineral components collected from deep sea water in water obtained by removing or reducing the mineral content from the collected marine mineral water. It is known to add minerals to obtain mineral containing mineral water drinks.

As described above, the method of ingesting and using the marine mineral water in the use of the marine mineral water is partially disclosed, but the method of using the marine mineral water for the skin is not known.

In the case of deep sea water, it has been reported that acute dermatitis patients have improved skin symptoms along with the imbalance of minerals when taking deep sea water, but the anti-allergic effect of marine mineral water has not been known.

However, in order to use mineral-rich water such as seawater or mineral water on the skin, various physical properties should be suitable for skin conditions.In natural skin, weak acid with pH of about 5.5, seawater has weak alkaline and marine properties with pH of 7.4 ~ 8.4. Mineral water is neutral or weak alkaline, so it is not suitable for use on the skin as it is, and in particular, atopic dermatitis, which is a representative skin disease, may have a rather adverse effect.

Therefore, in order to use marine mineral water, which is rich in minerals useful for the human body, to improve skin diseases such as atopic dermatitis, a process suitable for the skin as well as the composition ratio of the marine mineral water is required.

The problem to be solved by the present invention is to derive an optimal component ratio of the composition for inhibiting atopic dermatitis extracted from marine mineral water, and to provide a method for processing the composition to be suitable for the skin.

In order to achieve the above object, the present invention is obtained from 1,000,000 parts by weight of marine mineral water, sodium 8,900 to 9,300 parts by weight, calcium 1,400 to 1,800 parts by weight, magnesium 900 to 1,300 parts by weight, potassium 200 to 250 parts by weight, iron 20 to 25 Parts by weight, manganese 0.007 to 0.015 parts by weight, copper 0.008 to 0.016 parts by weight, zinc 8.00 to 9.00 parts by weight, lithium 0.080 to 0.100 parts by weight, strontium 25.0 to 35.0 parts by weight, germanium 0.001 to 0.002 parts by weight, selenium 0.250 to 0.350 parts by weight It provides a marine mineral water composition for suppressing atopic dermatitis, which comprises a mineral component of 0.250 to 0.350 parts by weight of vanadium, 0.007 to 0.015 parts by weight of molybdenum and 0.50 to 0.70 parts by weight of titanium.

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At this time, the ocher is preferably added so that the pH of the marine mineral water composition is 5.0 ~ 6.0.

First, the marine mineral water is collected and then filtered through a filter to remove impurities.

Next, the marine mineral water is dried to prepare the marine mineral water composition in powder form.

The drying is preferably dried by instant hot air while spraying the marine mineral water with a spray, there is almost no loss of minerals and deformation since moisture is evaporated by the instant hot air without continuous heating.

The composition is preferably ocher (黄土) is further added.

The composition powder is prepared from marine mineral water and generally has a neutral or weak alkalinity. Since natural skin shows a weak acidity of about pH 5.5, it is preferable to lower the pH of the composition powder to change it to a weak acidity such as skin condition. .

Ocher is a natural substance that can lower the pH while providing a useful effect on the skin. It is desirable to minimize the size of the ocher particles when the ocher is mixed with the composition. The finer the size of the ocher particles, the more the surface area per unit volume increases. It is possible to maximize the properties of adsorption, absorption and dehydration, suspension, and ion exchange of ocher.

There is a method of separating only the fine particles from the collected ocher, which is made of Jijangsu by suspending ocher in water, but when the ocher is suspended in water, most particles are aggregated and precipitate within a few tens of minutes. Is not.

As a solution to this problem, it is preferable to add sodium carbonate (Na ₂ CO ₃ ) to prepare Jijangsu. The sodium carbonate serves as a dispersant of ocher and suppresses agglomeration of ocher microparticles to maintain a dispersed state in water.

After mixing 5 to 15 parts by weight of ocher and 0.1 to 0.2 parts by weight of sodium carbonate to 100 parts by weight of water, stir it sufficiently and settle for 4 to 6 hours. The large particles sink to the bottom and the fine particles of loess are dispersed in water.

The ocher microparticles can be obtained by drying, pulverizing, and pulverizing the suspension in which the microparticles are dispersed.

In order to sterilize harmful bacteria or germs contained in ocher, it is preferable to add a process of heating the ocher of the fine particles to a low heat.

The amount of ocher added varies depending on the pH of the marine mineral water composition and ocher to be collected, and the ocher is added so that the pH of the mixture sufficiently mixed with the marine mineral water composition and ocher is 5.0 to 6.0.

In the method of adding ocher to the marine mineral water composition, the ocher powder may be added to the marine mineral water powder as described above, but the ocher fine particles before drying are dispersed in the marine mineral water from which impurities are removed before drying. It is also possible to add and dry together.

Marine mineral water composition according to the present invention contains abundant natural minerals and rare minerals to supply nutrients to the skin, in particular has the effect of inhibiting atopic dermatitis.

In addition, the weakly acidic composition can protect the natural sebaceous membrane, protect the skin from bacteria and improve the moisturizing ability to prevent drying of the skin surface.

1 is a graph measuring the ear thickness of the mouse to determine the TMA induced contact hypersensitivity inhibitory effect of the marine mineral water composition according to the present invention.
Figure 2 is a photograph of the mouse ear showing the TMA induced contact hypersensitivity inhibitory effect.
Figure 3 is a graph comparing the change in weight of lymph nodes of mice by TMA treatment.
Figure 4 is a graph showing the measurement of the amount of IgE and cytokine in the blood of the mouse by TMA treatment.
Figure 5 is a H & E staining and toluidine blue staining picture for confirming the morphology change of the tissue according to TMA treatment.

Hereinafter, the present invention will be described in detail.

The composition according to the invention is obtained from marine mineral water.

The present invention is obtained from 1,000,000 parts by weight of marine mineral water, 8,900 to 9,300 parts by weight, 1,400 to 1,800 parts by weight of calcium, 900 to 1,300 parts by weight of magnesium, 200 to 250 parts by weight of potassium, 20 to 25 parts by weight of iron, and 0.007 to 0.015 parts of manganese. Parts by weight, copper 0.008 to 0.016 parts by weight, zinc 8.00 to 9.00 parts by weight, lithium 0.080 to 0.100 parts by weight, strontium 25.0 to 35.0 parts by weight, germanium 0.001 to 0.002 parts by weight, selenium 0.250 to 0.350 parts by weight, vanadium 0.250 to 0.350 parts by weight The present invention relates to a marine mineral water composition for suppressing atopic dermatitis, which comprises from 0.007 to 0.015 parts by weight of molybdenum and 0.50 to 0.70 parts by weight of titanium.

Looking at the role of each component of the composition, sodium is an important component of the extracellular fluid, along with chlorine and bicarbonate ions to maintain the acid equilibrium of the body, to maintain the osmotic pressure of body fluids, maintain normal muscle reactions and body fluids Play a role.

Calcium is the most abundant element in the body's minerals, which can be used to treat osteoporosis, rickets, fractures, tooth decay, degenerative arthrosis, insomnia, calcium hypersensitivity, hypertension, arteriosclerosis, diarrhea, diabetes, allergic diseases, cold prevention, myocardial contractility Enhances the blood and promotes blood coagulation.

Magnesium is present in bones, muscles, soft tissues, and body fluids in the human body. As an important component of intracellular fluids, magnesium helps the synthesis of saccharides, lipids, protein metabolism and nucleic acids, and the synthesis of phospholipids in the body. It aids the metabolism of sodium, potassium and calcium, and has a calming effect on nervous system.

Potassium is the most important mineral for acid-alkali equilibrium of intracellular fluids, and it is the most important mineral for maintaining and supplying normal fluids, maintaining normal acid balance of the human body, maintaining normal muscle reactions, maintaining normal osmotic pressure, vasodilation, promoting insulin secretion, and myocardial infarction. Can be seen.

Iron is contained in hemoglobin and plays an important role in binding to oxygen. Iron deficiency can't produce hemoglobin, causing red blood cells to become smaller than normal, making the complexion worse and fatigued, and losing the balance of iron, causing anemia. do.

Manganese contributes to the activation of various enzymes, and exhibits the therapeutic effect of psychotic symptoms such as neurosis, bipolar disorder or schizophrenia, sexual enhancement, diabetes and asthma.

Copper is an essential trace element present in the body as part of several proteins, including enzymes. It transports stored iron and intestinal iron to hemoglobin-producing sites, and acts on the final process of the electron transport system in the mitochondria. is involved in the production of adenosine triphosphate, and also contributes to the normal maintenance of connective tissues of the skeletal and cardiovascular systems, and binds to the superoxide dismutase, an antioxidant enzyme, alone or in combination with zinc. It also serves to prevent oxidative damage.

Zinc is found in all somatic cells and is a component of over 200 enzymes that promote fetal growth, promote spermatogenesis, enhance skin resistance and treat dermatitis, promote growth and development, enhance insulin secretion, prevent atherosclerosis, and regulate thyroid function. .

Selenium is 1,970 times more antioxidant than natural tocopherols and is a component of glutathione peroxidase enzymes that inhibit the development and metastasis of cancer, prevent hypertension, detoxify heavy metals, antioxidant action, and anti-aging , Anti-cancer, cardiovascular disease prevention, liver cirrhosis prevention, inflammatory disease prevention, diabetes complication prevention, liver and kidney function enhancement, reproductive function enhancement, cataract prevention, immune function improvement.

Germanium is known for its blood purification and the ability to break down cholesterol attached to blood vessel walls and release it. Vanadium is associated with cholesterol, cardiovascular and renal disease inhibition.

Since the composition according to the present invention is obtained from marine mineral water, the marine mineral water is generally neutral or slightly alkaline, so in order to apply the composition to the skin, it is necessary to adjust the pH to weak acidity similar to the skin.

Ocher is one of the materials that shows acidity and benefits your skin.

Ocher is a natural material that contains oxygen with a lot of fine constituent particles, shows excellent purification ability due to its structural characteristics, features of far-infrared radiation upon heating, and synergistic effect of microorganisms and various enzymes in ocher. .

The chemical composition of the loess is similar to that of alluvial soils, with 60 to 65% silica (SiO ₂ ), 5 to 6% iron, 10 to 13% alumina (Al ₂ O ₃ ), about 2% magnesium and sodium, and 1.5 potassium % And about 8% of lime.

In addition, the ocher is mainly made of fine sand and has a large amount of calcium carbonate (CaCO ₃ ). The clay has a viscous force and is viscous, and changes to clay when water is added. .

In particular, ocher contains catalase enzymes that remove hydrogen peroxide and protease enzymes that hydrolyze proteins into amino acids, which neutralize lipid peroxide, a toxin in the body, and cause cancer, boils and other decayed cells in organisms. It is weak to decompose.

In addition, far-infrared rays emitted from the ocher to expand the capillaries to facilitate blood circulation and promote metabolic functions to help prevent disease and relieve stress, and has the effect of releasing toxins in the intestines.

The ocher in the present invention has the effect of clearing the skin by adsorbing impurities of the skin as well as regulating the pH of the marine mineral water composition as described above, and when contacted with the skin, heat received from the body temperature is transferred to the ocher and When heated, far infrared rays are emitted.

The far infrared rays penetrate deep into the skin to generate heat, which helps to eliminate bacteria that cause various diseases, and expands capillaries to help blood circulation and tissue formation, and also activates cellular tissue It is also effective in preventing various adult diseases such as anti-aging, promoting metabolism, and chronic fatigue.

As described above, the marine mineral water composition, which is controlled to be slightly acidic by ocher, is particularly effective for atopic skin diseases because it is provided with a function of protecting the natural sebaceous membrane of the skin, maintaining moisture, and protecting the skin from bacteria.

Hereinafter, the present invention will be described in detail based on the following examples.

It should be noted, however, that the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention. The present invention is not limited to the following examples. Will be apparent to those skilled in the art to which the present invention pertains.

Example 1 Preparation of Marine Mineral Water Composition

After excavating a rock of 1100m underground near Geumjin-ri, Okgye-myeon, Gangneung-si, Gangwon-do, Korea, the mineral water was collected and filtered through a 1.0㎛ filter and supplied to a spray drying apparatus (L-8, manufactured by OHKAWARA KAKOHKI, Japan).

The mineral water supplied to the spray drying apparatus is sprayed into fine particles by a built-in disk rotating at a speed of 12,000 rpm, and at the same time as the spraying process, hot air at 180 ° C. is applied to evaporate moisture to obtain a dry powdered mineral composition. Lose.

<Test Example 1> Comparison of Mineral Analysis of Marine Mineral Water, Surface Water, and Deep Sea Water

The mineral component of the composition of Example 1 was investigated and compared with the mineral component of the surface water and the deep sea water, and is shown in Tables 1 and 2 below.

The analysis was commissioned by analytical organizations such as Korea Research Institute for Chemical Testing, Kangwon-do Institute of Health and Environment, Korea Food and Drug Administration, Japan Food Analysis Center, and ANRESCO in the United States. The results were compared in terms of Mass Minerals (Table 1) and Micro Minerals (Table 2).

Mass mineral comparison results of marine mineral waters, surface waters and deep ocean waters Analysis item Example 1 (mg / L) Ocean surface water (mg / ℓ) Deep Sea Water (mg / ℓ) salt 9070 10,500 10,767 calcium 1,600 401 414 magnesium 1,100 1,300 1,320 potassium 224 380 410

The marine mineral water of Example 1 has a similar salt concentration compared to the surface water of the sea and the depth of the sea, but shows a big difference in mineral composition. There was no significant difference in the mineral composition between the surface water and the deep sea water.

To explain this in more detail, calcium and magnesium have been reported to reduce the blood pressure and prevent myocardial infarction by ingesting the appropriate concentration, the calcium concentration of Example 1 was very high compared to the surface water and the deep sea water. In particular, the ratio of calcium and magnesium concentrations of Example 1 was close to the ratio (1.6: 1) known to be ideal for human absorption.

However, the surface waters of the oceans and the deep seas have excessive amounts of sodium and magnesium compared to other minerals, and the ratio of calcium and magnesium in the ratio of about 1: 3 can burden the human body and cause thirst if drinking. If you wash your face or take a bath, it will remain sticky.

Trace mineral comparison results of marine mineral waters, surface waters and deep ocean waters Analysis item Example 1 (mg / L) Ocean surface water (mg / ℓ) Deep Sea Water (mg / ℓ) iron 23 0.01 0.023 manganese 0.011 0.002 0.002 Copper 0.012 0.0006 0.0004 zinc 8.46 0.01 0.009 lithium 0.089 ND ^{Note 1)} ND strontium 30.4 8 7.54 germanium 0.0014 ND 0.0005 Selenium 0.302 ND ND vanadium 0.305 ND ND molybdenum 0.011 ND 0.01 titanium 0.62 ND ND

Note 1) ND: Not Detected

The marine mineral water of Example 1 contains a very high amount of trace minerals as compared to the surface water of the sea and the deep sea water, and particularly has a high concentration of selenium and vanadium.

That is, it can be seen that the mineral composition is varied, and minerals such as iron, manganese, copper, and zinc are present in abundance, and there are many rare minerals, such as selenium and vanadium, which are not detected in general water.

Test Example 2 Verification of Inhibitory Effect on Atopic Inflammation

1) Sample preparation and laboratory animal breeding

First, the mineral composition obtained in Example 1 was dissolved in purified water to prepare a composition sample having a concentration of 0.7 and 2.0 wt.%.

Next, 6-week-old female Balb / c mice were purchased from Nara Biotech.

The temperature of the feeding room was 22 ± 1 ℃ and the humidity was 55 ± 5%, and the photoperiod and dark cycle were adjusted to 12 hours daily. Feed and water were taken freely.

2) TMA induced contact hypersensitivity

The trimellitic anhydride (TMA) induced atopic model mouse was used by the method of Scineider et al. (Schneider C, Dose W, Z0llner TM and Rose L. Chronic mouse model of YMA-induced contact hypersensitivity. Journal of Investigative dermatology ( 2009) 129: 899-907).

In the contact hypersensitivity reaction (CHS) model using Balb / c mice, the inflammation was induced by TMA to increase the thickness of the ear, and the TMA-induced inflammation was treated with the composition sample. The efficacy of the invention was verified.

50 μl of 5% TMA was applied to the hairless mice's flanks on the day 0 for the model mouse, and 5 μl TMA was applied to the back of both ears 5 days after the first sensitization, and then from 6 to 17 days. Every day 10 μl of 2% TMA was applied to the back of both ears.

After the TMA application, a solution of acetone: isopropylmyristate in 4: 1 (v / v) was applied to form a TMA treatment group.

In the TMA treatment group, one hour after application of TMA and acetone-isopropyl myristate, 10 μl of each of the 0.7 and 2.0 wt.% Composition samples was applied to the 0.7% treatment group and the 2.0% treatment group.

In addition, the model mice not treated with TMA as a normal group for comparison, the TMA treated group and the normal group was applied with phosphate buffered saline (PBS) instead of the composition sample.

Ear thickness was measured using a micrometer immediately before application of the sample from day 6 to day 17, and is shown in FIG.

The TMA, acetone and isopropyl myristate were purchased from Sigma Aldrich Korea.

As shown in Figure 1, the TMA control group (Control) is compared with the normal group (Normal) it can be seen that the overall thickness of the ears, 0.7% treatment group treated with 0,7% composition samples after 8 days ( 0.70%) and 2.0% treated groups (2%) treated with 2% composition samples inhibited the increase in ear thickness compared to the TMA treated group.

In addition, in the inflammatory response accompanied by peripheral blood outflow due to vasodilation as well as the thickness of the ear, as shown in FIG. 2, the TMA control group (Control) appears red in the color of the ear, while 0.7% treatment group (0.7%) and 2.0 In the% treatment group (2%), the red color of the ears decreased compared with the TMA treatment group, although not normal.

When the 0,7% treatment group and the 2.0% treatment group showed similar effects of increasing the thickness of the ear and inhibiting peripheral blood outflow, the treatment with the marine mineral water composition of 0,7% concentration according to the present invention showed sufficient inhibitory effect on inflammation. It can be seen that.

3) Changes in the weight of lymph nodes by TMA treatment

On day 17, cervical lymph nodes of the lower right ear of the mouse were isolated and weighed with an electronic balance, and the results are shown in FIG. 3.

In contact hypersensitivity by the TMA treatment, the weight of the cervical lymph nodes increases due to the proliferation of lymph node cells and the influx of immune cells due to an increase in the immune response.

As shown in FIG. 3, the cervical lymph node weight of the TMA-treated group (Control) increased about 8-fold compared to the normal group (Normal). However, in the 0.7% treated group (0.70%) and 2.0% treated group (2%) treated with marine mineral water composition samples, the lymph node weight was significantly decreased.

From the above results, it is expected that the treatment of the marine mineral water composition according to the present invention will suppress the apoptotic response by inhibiting the immune action in the lymph nodes.

4) Changes in Blood IgE and Cytokines by TMA Treatment

On the 17th day of sensitization, the right ear of the mouse was cut out and blood was collected from the heart.

Homogenize the ears of the mouse with 2 ml of PBS, centrifuge for 20 minutes at 25000 g of centrifugal force, remove the supernatant, and use cytokine (cytokines) using an ELISA kit (manufactured by BD Bioscience, Germany). The amount of was measured.

In addition, after separating serum from the blood of the mouse, the amount of immunoglobulin E (immunoglobulin E: IgE) was measured using the ELISA kit, and the results are shown in FIG. 4.

In most patients with atopic dermatitis, blood IgE is increased and contact hypersensitivity induced by TMA can be used as a unit of measure for humoral immunity under Th ₂ conditions.

In addition, TMA-induced acute hypersensitivity hypersensitivity in Balb / c mice showed a marked increase in the expression of cytokines in the ear, IL-4 in chronic response, and TNF-α in acute response. Results are being reported to the academic community.

As can be seen in Figure 4 TMA control group (Control) significantly increased IgE compared to the normal group (Normal), 0.7% treated group (0.70%) and 2.0% treated group (2) treated with a marine mineral water composition samples (2) %) Significantly decreased.

In cytokine inflammatory precursors, IL-4 and TNF-α decreased, but decreased slightly, and IL-6 showed a marked decrease.

Judging from the above results, the inhibitory effect on the contact hypersensitivity reaction of the marine mineral water composition according to the present invention contributes to the inhibition of the production of IL-6 during the inflammation-promoting reaction, IL-4 or TNF-α is partly involved Judging.

5) tissue staining

Morphological changes and subcutaneous cell influx in inflamed skin can be distinguished by histological or immunohistochemical examination.

In the TMA-induced chronic contact hypersensitivity model, strong cell influx and acanthosis are observed, especially basophils with hematoxylin and eosin.

In this test example, the morphology of the tissue was observed by staining with hematoxylin-eosin and toluidine blue.

On the 17th day of sensitization, the ears were cut out and placed in 4% paraformaldehyde. After 1 day, the tissues were dehydrated in 30% sucrose for 2 days, and the dehydrated tissues were placed in a cell freezing medium.

Using a cryostat (manufactured by Leica microsystems, Germany), the tissue cut out to a thickness of 6 mm is dried on a slide, and then washed with a distilled water, and the cell freezing medium is washed. Hematoxylin and 0.5% eosin ( H & E staining was performed using eosin), and toluidine blue staining was performed using toluidine blue, and is shown in FIG. 5.

5 a to d are H & E stained pictures, and e to h of FIG. 5 are toluidine blue stained pictures.

5 a to d of the H & E staining, the TMA control group (Control) was observed that the tissue is about three times thicker than the normal group (Normal) and the tissue morphology is broken.

In the 0.7% treated group (0.7%) and 2.0% treated group (2%), it was confirmed that the thickness of the tissue was reduced compared to the TMA treated group, and the tissue morphology was found to be similar to the normal group in a concentration-dependent manner.

In addition, in the e-h of FIG. 5, which stained granulocytes such as mast cells using toluidine blue, 0.7% treated group (0.7%) and 2.0% treated group (2%) were purple compared to TMA treated group (Control). It was confirmed that the number of granulocytes stained with greatly reduced.

In view of the above results, the marine mineral water composition according to the present invention has an inhibitory effect on the influx of granulocytes, tissue necrosis, etc. in TMA-induced contact hypersensitivity reactions, and thus the inflow of granulocytes in atopic hypersensitivity by contact. It can be estimated that there is an inhibitory action by.

Summarizing the results of Test Example 2, the marine mineral water composition according to the present invention partially inhibited peripheral blood outflow due to vascular expansion induced by TMA, and somewhat suppressed edema, which is a typical symptom of the inflammatory response.

In addition, the weight of cervical lymph nodes was decreased by treatment of marine mineral water composition in TMA induced CHS reaction, suggesting that the increased immune response in contact hypersensitivity by TMA induction is partially reduced by treatment of marine mineral water composition. .

The most pronounced phenomena in hypersensitivity reactions were the increase in blood IgE. In the CHS induced by TMA, the blood IgE was increased, and blood IgE was markedly decreased after treatment with marine mineral water.

In addition, cytokine in the tissues showed a significant decrease in IL-6 in the marine mineral water treated group, and other inflammatory precursors or Th2 cytokines, TNF-α or IL-4, showed a slight decrease.

TMA staining with hematoxylin, eosin, and toluidine blue revealed distinct tissue edema by TMA and influx of granulocytes into the tissue. However, the treatment of marine mineral water significantly reduced edema and the influx of granulocytes into the tissues.

In all tests, the 0.7% and 2.0% treatment groups showed similar inhibitory effects, indicating that 0,7% treatment had sufficient inhibitory effects.

Example 2 Addition of Ocher

30 kg of the collected ocher and 450 g of sodium carbonate were mixed in 300 kg of water, and the mixture was stirred well. After standing for 5 hours, the suspension was poured out along the upper suspension, dried, and ground to obtain fine loess.

10 kg of the mineral composition prepared in Example 1 was mixed with 550 g of the fine loess so that the pH of the mixture was 5.5.

<Test Example 3> Moisturizing power measurement

In the aqueous solution containing 10 wt.% Of the composition of Example 1, the aqueous solution containing 10 wt.% Of the composition of Example 2 and purified water, 10 g of the epidermal cells of sufficiently dried human body was immersed for 24 hours to be saturated and absorbed.

The saturated absorbed epidermal cells were taken out and weighed, followed by air drying for 48 hours, and the results are shown in Table 3 below.

Moisturizing power measurement result for epidermal cells Aqueous solution of Example 1 Aqueous solution of Example 2 Purified water Saturated Absorption Weight (g) 18.3 18.4 18.3 Dry weight (g) 15.5 16.1 13.9

As shown in the results of Table 3, it can be seen that the water moisturizing capacity of the aqueous solution of Example 1 and Example 2 according to the present invention is significantly higher than that of purified water, and in particular, Example 2 added with ocher to the marine mineral water composition It can be seen that the moisturizing ability is more improved than Example 1 without adding ocher.

As described above, the marine mineral water composition according to the present invention inhibited to some extent peripheral edema caused by TMA-induced vascular dilatation and edema, which is a typical symptom of an inflammatory response, thereby reducing the weight of lymph nodes and irritating immunity. Inhibition of the reaction to inhibit the concentration of IgE in the blood, inhibit the production of inflammatory precursors IL-6, etc. to reduce inflammation, inhibiting the influx of granulocytes, such as mast cells in local tissues of contact hypersensitivity reactions It can be seen that the marine mineral water composition according to the invention has some inhibitory effect on the atopic inflammatory response.

In addition, by adding ocher to the composition, the pH is converted to weak acidity to protect the natural sebaceous membrane, to protect the skin from bacteria, and to improve the moisturizing ability to prevent the drying of the skin surface, thereby protecting the atopic skin with severe lack of moisture on the skin surface. You can expect the effect.

Claims (6)

Obtained from 1,000,000 parts by weight of marine mineral water,
Sodium 8,900-9,300 parts by weight, calcium 1,400-1,800 parts by weight, magnesium 900-1,300 parts by weight, potassium 200-250 parts by weight, iron 20-25 parts by weight, manganese 0.007-0.015 parts by weight, copper 0.008-0.016 parts by weight, zinc 8.00 to 9.00 parts by weight, lithium 0.080 to 0.100 parts by weight, strontium 25.0 to 35.0 parts by weight, germanium 0.001 to 0.002 parts by weight, selenium 0.250 to 0.350 parts by weight, vanadium 0.250 to 0.350 parts by weight, molybdenum 0.007 to 0.015 parts by weight and titanium 0.50 A marine mineral water composition for inhibiting atopic dermatitis, comprising -0.70 parts by weight of mineral components. delete The method according to claim 1,
The mineral component is a marine mineral water composition for inhibiting atopic dermatitis, characterized in that the intake of marine mineral water and then filtered with a filter and dried to form a powder. The method according to claim 3,
The drying is a marine mineral water composition for inhibiting atopic dermatitis, characterized in that the drying by instant hot air while spraying the marine mineral water with a spray. The method according to claim 1,
It characterized in that the ocher is further added so that the pH of the marine mineral water composition is 5.0 ~ 6.0, marine mineral water composition for inhibiting atopic dermatitis. The method according to claim 5,
The ocher is mixed with 100 to 5 parts by weight of ocher and 0.1 to 0.2 parts by weight of sodium carbonate, and then left for 4 to 6 hours, followed by a suspension and drying and pulverizing, characterized in that atopic dermatitis Inhibiting marine mineral water composition.

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