KR101186905B1 - Degumming method of bast fiber using bacillus coagulans hp-10 - Google Patents

Abstract

PURPOSE: A method for refining bast fibers with excellent functionality using Bacillus coagulans HP-10 is provided to refine the bast fibers of flax, ramie, jute, and hemp and to obtain the bast fibers of high quality. CONSTITUTION: A method for refining bast fiber using Bacillus coagulans HP-10(deposit number KCTC 10108BP) comprises: a step of putting 250 parts of bast fiber ingredients, 3,000-7,000 parts of purified water, and 80-120 parts of 0.01-1% sodium carbonate solution by weight and pre-treating the at 10-20 Deg. C. for 5-6 hours; a step of removing liquid and putting 3,000-7,000 parts of purified water, 90-110 parts of Bacillus coagulans HP-10 culture liquid, 3-10 parts of pectin, 3-10 parts of ammonium sulfate((NH_4)_2SO_4), 3-10 parts of sodium chloride, 5-30 parts of potassium dihydrogen phosphate, 90-110 parts of pupa extract, and 3-10 parts of Tween 80 by weight to 250 parts by weight of the pre-treated bast fiber ingredient; a step of primarily refining at 40-55 Deg. C. and at neutral pH concentrate for 20-40 hours; a step of removing liquid; a step of washing 250 parts by weight of the bast fiber with 5,000-15,000 parts of purified water for secondary refining, foaming agent, 20-40 parts of sodium hydrogen carbonate, 20-40 parts of 30% hydrogen peroxide, 20-40 parts of sodium percarbonate, and 20-40 parts of sodium carbonate by weight at 70-90 Deg. C. for 20-40 minutes. [Reference numerals] (AA) (Process 1) step of inputting 250 parts by weight of bast fiber ingredient, 3,000-7,000 parts by weight of purified water, and 80-120 parts by weight of 0.01-1% sodium carbonate solution and pretreating at 10-20°C for 5-6 hours; (BB) (Process 2) step of removing liquid of pretreatment step; injecting 3,000-7,000 parts by weight of purified water, 90-110 parts by weight of microorganism culture liquid of Bacillus coagulans HP-10(deposit number KCTC 10108BP), 3-10 part by weight of pectin as a microorganism nutrient source, 3-10 parts by weight of ammonium sulfate(NH_4)_2SO_3 ammonium sulphate), 3-10 parts by weight of sodium chloride(NaCl), 5-30 part by weight of potassium dihydrogen phosphate, 90-110 parts by weight of pupa extract, and 3-10 parts by weight of Tween 80 to 250 parts by weight of pretreated bast fiber ingredients of process 1; controlling to 40-55°C and neutral pH; and primarily refining for 20-40 hours; (CC) (Process 3) step of removing liquid of the primary refining step; adding 5,000-15,000 parts by weight of purified water for secondary refining, 0.01-1 wt% of foaming agent based on the weight of the purified water, 20-40 parts by weight of sodium hydrogen carbonate, 20-40 parts by weight of 30% hydrogen peroxide, 20-40 parts by weight of sodium hydroxide, and 20-40 parts by weight of sodium percarbonate to 250 parts by weight of primarily refined bast fiber ingredients; and washing at 70-90°C for 20-40 minutes and secondarily refining.

Description

Degumming method of bast fiber using Bacillus coagulans HP-10}

The present invention Bacillus coagulans HP-10 ( Bacillus coagulans HP-10, microorganism accession number KCTC10108BP) strain relates to the refining method of bast fiber.

More specifically, the present invention relates to a method for refining bast fibers having excellent basal fiber recovery and functionality using Bacillus coagulans HP-10 strain capable of effectively degrading pectin and lignin on the surface of hemp.

Bast fiber is a fiber contained in the outer tissue of the stem forming layer of the plant, and consists of phloem fiber, cortical fiber, and the like. Bast fiber is well developed in hemp, flax, paper mulberry, cedar, vine, mulberry, sycamore, etc. The soil or climatic conditions of our country is suitable for mass cultivation of the plants. Although the bast fiber in the natural state is not suitable for use as a fiber, the bast fiber has been refined using an alkali chemical such as sodium hydroxide (NaOH) so as to be processed into a soft fiber.

However, this traditional method uses a large amount of strong chemicals, which impairs the intrinsic properties of the fiber and does not sufficiently remove the impurities such as pectin in the fiber, thereby making it impossible to obtain high quality bast fibers and wastewater that is harmful to the environment. It caused a problem such as occurring. As a replacement for this, various techniques for refining using microbial cultures have been developed, and the present inventors have developed a method for refining bast fiber of tough plants by refining the bast fibers of tough plants by allowing aerobic and anaerobic bacteria to coexist. Patent No. 317476) ”and the microorganisms present in hemp to separate“ enterobacter low-goal HI.EL 2006 useful for refining bast fibers and methods for refining bast fibers using the same (Korea Patent No. 704726) ”and“ enterobacter Has developed a method for refining bast fiber using low-going HI 2006 (Korea Patent No. 961886).

Therefore, the present inventors have a better basal fiber recovery and functionality of bast fiber by using Bacillus coagulans HP-10 that can effectively decompose the pectin and lignin on the hemp surface than when using Enterobacter low gobi HI 2006 The present invention was completed by revealing that it can be refined.

Korean Registered Patent No.317476 (Method of refining bast fiber of toughened plant, Dec. 22, 2001) Korean Registered Patent No. 704726 (Enterobacter low gobi H.L. 2006 useful for refining bast fiber and method for refining bast fiber using the same, Announcement 2007.04.09.) Korean Registered Patent No. 961886 (Method of refining bast fiber using Enterobacter low gobi HEL 2006, Announcement 2010.06.09.)

The object of the present invention is Bacillus Coagulans HP-10 ( Bacillus coagulans HP-10, microbial accession number KCTC10108BP) strain to provide a method for refining bast fibers.

More specifically, an object of the present invention is to provide a method for refining bast fiber having excellent basal fiber recovery and functionality using Bacillus coagulans HP-10 strain that can effectively decompose pectin and lignin on the surface of hemp. have.

The present invention Bacillus coagulans HP-10 ( Bacillus coagulans relates to a HP-10, microorganism accession number KCTC10108BP) refining method of the bast fibers using the strain.

In detail, the refining method of the bast fibers,

(Step 1) 250 parts by weight of bast fiber raw material, 3,000 ~ 7,000 parts by weight of purified water and 80 ~ 120 parts by weight of 0.01% to 1% sodium carbonate aqueous solution in a water bath and then pretreatment for 5-6 hours at a temperature of 10 ~ 20 ℃ ;

(Step 2) remove the liquid of the pretreatment step in the water tank,

250 parts by weight of pre-treated bast fiber raw material in one step 3,000 to 7,000 parts by weight of purified water, 90 to 110 parts by weight of Bacillus coagulans HP-10 microbial culture, 3 to 10 parts by weight of pectin as a microbial nutrient, ammonium sulfate ((NH ₄ ) ₂ SO ₄ , yuan) 3-10 parts by weight, sodium chloride (NaCl) 3-10 parts by weight, potassium hydrogen hydrogen phosphate 5-30 parts by weight, pupa extract 90-110 parts by weight and Tween 80 3-10 Step by weight and adjusting the conditions of the temperature of 40 ~ 55 ℃ and neutral pH for the first refining for 20 to 35 hours; And,

(Step 3) remove the liquid of the first refining step in the water tank,

In the second step, 250 parts by weight of the first refined bast fiber raw material is washed again with 5,000-15,000 parts by weight of purified water at 70-90 ° C. and 0.01-1% by weight of foamed water and bleach by weight of purified water for 20-40 minutes. It characterized in that it comprises a step.

The bast fiber raw material may be one selected from the group consisting of flax, jute, jute and hemp.

In the first refining step of (step 2), mechanical shaking may be further performed to increase the efficiency of refining, and the surfactant may be added in an amount of 0.01 to 1% by weight of the weight of the purified water.

After the second refining step of (step 3), the third refining step of separating the bast fibers, washing with clean water and refining may be further performed.

In addition, the present invention provides a bast fiber refined using the method, and provides clothing, bedding products, automotive interior products, building interior products, etc. manufactured using the bast fibers.

Hereinafter, the present invention will be described in detail.

In the method for refining bast fibers using Bacillus coagulans HP-10 according to the present invention, in the pretreatment step of the bast fiber refining method (step 1), sodium carbonate has a concentration of less than 0.01% or the amount of 80 parts by weight. When used less than the amount of sodium carbonate may have a problem that the primary refining effect by the microorganism according to the present invention is not sufficient. Conversely, if the concentration exceeds 1% or exceeds 120 parts by weight, it may not be economical and may adversely affect the quality of the fiber finally obtained.

In addition, when the temperature is less than 10 ° C or less than 5 hours in the pretreatment step (step 1), the pretreatment effect may not be sufficient. It is not preferable in terms of economics and productivity that the temperature exceeds 20 ° C. or more than 6 hours, but the present invention is not limited thereto. It will be apparent to those skilled in the art that the pretreatment temperature and time can be adjusted according to the condition of the bast fiber raw material and the environment such as the external temperature.

The medium composition of the Bacillus coagulans HP-10 microorganism culture medium of step (2) is 10-30 parts by weight of pectin, 3-10 parts by weight of ammonium sulfate (yarn), 1-5 parts by weight of yeast extract, 3-10 weight of peptone Part, but may include 3 to 7 parts by weight of sodium chloride, but the conditions of the composition is not limited thereto, it will be understood by those skilled in the art that methods and media according to conventional expansion culture can be used.

The Bacillus coagulans HP-10 strain is immersed hemp in a constant temperature water tank of 40 ~ 55 ℃, shake cultured for 20 to 50 hours to increase the microorganisms attached to the surface of the hemp, and then microorganisms having excellent pectin degradation ability After separation, it can be obtained by pure culture at 40 ~ 55 ℃. The culture solution for culturing the microorganisms by immersing the hemp may have the same medium composition as the microbial culture solution contained in the primary refining solution (step 2).

The Bacillus coagulans HP-10 is an aerobic or breathable anaerobic bacillus and the cell size is 0.6 ~ 1.0 × 1.2 ~ 1.5㎛ oval. The Bacillus coagulans HP-10 grows well at 33 ~ 55 ℃, preferably grows best at 35 ~ 45 ℃.

When the Bacillus coagulans HP-10 culture medium is used in less than 90 parts by weight in the first refining step of (2 step), the amount is insufficient, the refining effect may be lowered and refining may take a long time. On the other hand, exceeding 110 parts by weight does not have a particular problem, but the more the refining effect is increased, but the cost is not reasonable. In addition, when the reaction temperature is maintained at less than 40 ℃ may be a problem that the activity of the strain according to the present invention is lowered and the refining effect is also lowered and the time required for refining. It may also have a problem that the strain according to the present invention and the enzyme produced therefrom loses activity beyond 55 ° C., thereby reducing the efficiency of refining.

The pupa extract of the (2 step) is finely chopped pupa, after adding the water of 2 ~ 10 times the weight of the pupa cut, and then can use the filtrate extracted for 2 to 10 hours at 80 ~ 120 ℃. At this time, the solid content of pupa can be filtered using burlap, fine sieve, nonwoven fabric, filter paper and the like.

In the first refining step of the (step 2) step may be further performed using a conventional mechanical shaking (stirring) to increase the efficiency of refining.

In addition, in this step, a surfactant such as Tween 80 may be further added in an amount of 0.01 to 1% by weight based on the total weight of the primary refining liquid, and such a surfactant may be easily fibrous with the strain and enzyme. It is possible to penetrate the inside and to efficiently process impurities. That is, the degradation products and other impurities of pectin and lignin, which are degraded and separated from the tissue, are prevented from penetrating into the fibrous tissue or sticking to the surface of the fibrous tissue. When the surfactant is used in less than 0.01% by weight compared to the purified water, the function of the surfactant is not sufficient, there is a problem that the infiltration effect of the strain and the separation effect of impurities is insufficient. If it exceeds 1% by weight there is no particular problem, but there may be a problem that the price competitiveness is lowered. In the above, Tween 80 is a trade name made by a US company called Uniqema, and is a surfactant in which a hydrophilic group of polyethyleneglycol and a hydrophobic group of hydrocarbon are mixed.

Glycolytic enzymes may be added to the primary refining liquid of the above (second step) for a more effective refining step. Glycolytic enzymes can complete a more efficient refining process by reducing the biological process time to decompose pectin or lignin, which protects the cell walls of plants such as horse crops. Glycolytic enzymes usable in the present invention may be freely used components well known in the art, for example, may include amylase, pectinase, ligninase, and the like. have.

In addition, lipase may be used as a lipolytic enzyme for the preparation of higher purity bast fibers, and silanase and glue, which break down fibrous tissues such as xylan and glucan, which are main components of hemicellulose, may be degraded. Glucanase and the like can also be used.

Appropriate charge concentration of each enzyme can be added as 1 × 10 ^-6 ~ 0.01 wt% based on the primary refining liquid.

As for the purified water of said (3 step), it is preferable to use 1-3 times of the volume of purified water of (2 step).

When the foaming agent and the bleaching agent used in the second refining step of (3 step) are included in less than 0.01% by weight, there may be a problem that the washing amount (refining effect) is not greatly improved because the amount is not sufficient. When it exceeds 1% by weight, it may adversely affect the physical properties of the bast fiber obtained. The type of foaming agent and bleaching agent is mixed with sodium hydrogen carbonate (NaHCO ₃ ), hydrogen peroxide (H ₂ O ₂ , 30% solution), sodium hydroxide (NaOH) and sodium percarbonate (2Na ₂ CO ₃ ㆍ 3H ₂ O ₂ ) use. Each mixing ratio includes 20 to 40 parts by weight of sodium bicarbonate, 20 to 40 parts by weight of hydrogen peroxide, 20 to 40 parts by weight of sodium hydroxide, and 20 to 40 parts by weight of sodium percarbonate, and the use of the foaming agent and the bleach mixture. This can greatly improve the recovery and quality of bast fibers.

In addition, the third refining step, it is preferable to use 1 to 3 times the volume of purified water in (2 step), preferably can be carried out for 1 to 48 hours at 30 ~ 70 ℃.

Meanwhile, bast fibers produced using the present invention can be used in various fiber products. The textile products include clothing, bedding supplies, automotive and building interior materials, air cleaners, various filters, and the like.

The present invention is to use the Bacillus coagulans HP-10 strain ( Bacillus coagulans HP-10, microbial accession number KCTC10108BP) that can effectively decompose pectin and lignin on the surface of hemp, to refining bast fiber with excellent bast fiber recovery and functionality The method relates to the Bacillus coagulans HP-10 is immersed hemp in a constant temperature water bath of 40 ~ 55 ℃, shaking culture for 20 to 50 hours to increase the microorganisms attached to the surface of the hemp, pectin resolution After separating the excellent microorganism can be obtained by pure culture at 40 ~ 55 ℃. The Bacillus coagulans HP-10 was a heat-resistant bacterium that was able to prevent contamination of decaying strains growing at 40 ° C. or lower during refining, and also because the enzymes produced by the strains do not lose their activity due to heat. Can be recycled. The bast fiber refined using the Bacillus coagulans HP-10 was less than 5% of the residual amount of pectin and lignin, thereby increasing the recovery of bast fiber to 95% or more. Therefore, through the method of the present invention can be usefully used for refining bast fibers such as flax, jute, jute, hemp, it is possible to obtain a high quality bast fiber with a high recovery of bast fiber using an improved refining method.

1 is a flow chart showing a method for refining bast fibers using Bacillus coagulans HP-10 according to Example 1 of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Rather, the embodiments introduced herein are intended to provide a thorough and complete disclosure and to fully convey the spirit of the invention to those skilled in the art.

< Example  One. Bacillus Coagulance HP Separation and Identification of -10>

Bacillus coagulans HP-10 strain used in refining bast fiber of the present invention is mixed with 1 kg of hemp medium (1 kg pectin, 0.5 kg ammonium sulfate, 0.3 kg yeast extract, 0.7 kg peptone, 0.7 kg sodium chloride with 10 kg water) and sterilized Immersed in a constant temperature water bath of 50 ℃ containing a medium prepared by, and shaking culture for 40 hours to enrich the microorganisms attached to the surface of the hemp, and after separating the microorganisms excellent in pectin resolution, and pure culture at 50 ℃ It was deposited with the accession number KCTC 10108BP to the Korea Institute of Bioscience and Biotechnology.

The Bacillus coagulans HP-10 is an aerobic or breathable anaerobic bacillus and the cell size is (0.6 ~ 1.0) ㎛ × (1.2 ~ 1.5) ㎛ oval. The Bacillus coagulans HP-10 grows well at 33 ~ 55 ℃, preferably grows best at 35 ~ 45 ℃.

< Example  2. Refining Bacillus Coagulance HP Preparation of -10 Culture>

The Bacillus coagulans HP-10 culture medium was mixed with 10 kg of pectin, 5 kg of ammonium sulfate (Yuan), 3 kg of yeast extract, 7 kg of peptone, and 7 kg of sodium chloride with 100 kg of water, and the Bacillus coagulans strain isolated in Example 1 on sterile medium. 10 g of pellets were inoculated and incubated at 40 ° C. for 5 days to prepare.

< Example  3. Bacillus Coagulance HP With -10 Bast fiber  Manufacturing>

First, 250 kg of hemp as a bast fiber material was added to the tank, and 5t of purified water was added thereto, and then 100 kg of an aqueous sodium carbonate solution of 0.1% concentration was added thereto, followed by pretreatment at 15 ° C. for 5 hours. Then, the pretreatment liquid was removed, and 5t of fresh purified water was added thereto, and 100 kg of Bacillus coagulans HP-10 culture medium of Example 3 was added. As a microbial nutrient, 100 kg of pupa extract, 7 kg of pectin, and ammonium sulfate (Yuan) ) 5 kg, sodium 7 kg, 20 kg potassium II hydrogen phosphate and 7 kg Tween 80 were added. Then, the refinement was performed for 30 hours while slowly adjusting the temperature of the purified water to a temperature of 50 ℃. As a pupa extract, 150 liters of water was added to the pupa 30 kg taken out from the cocoon, and the filtrate was filtered through burlap from 90 to 8 hours.

After the first refining step was completed, the liquid components except for the first refined bast fiber were removed, and 10t of purified water at 80 ° C. was further added, followed by 10 kg of sodium bicarbonate, 10 kg of hydrogen peroxide, and 10 kg of sodium hydroxide as a foaming agent. And 10 kg of sodium percarbonate was added and washed for 1 hour to perform a secondary refining.

The bast fiber after the secondary refining was recovered from the water bath and washed once more for 24 hours with clean water at 65 ° C. to perform the tertiary refining to obtain the bast fiber refined.

< Comparative example  Enterobacter Low rain HI  Preparation of 2006 Culture>

To prepare a strain culture in the same manner as in Example 2, but instead of the Bacillus Coagulans HP-1 strain Enterobacter low gobi HL 2006 ( Enterobacter gergoviae HL 2006, Accession No. KCTC 10943BP).

< Comparative example  2. Enterobacter Low rain HI  With 2006 Bast fiber  Manufacturing>

The bast fibers were refined in the same manner as in Example 3, but instead of the culture solution of Bacillus coagulans HP-1 of Example 2, the culture solution of Enterobacter low gobi HIEL 2006 of Comparative Example 1 was used.

< Comparative example  3. Bacillus Maseran's KH91  Preparation of Culture Solution>

To prepare a strain culture in the same manner as in Example 2, Bacillus maserans KH91 ( Bacillus instead of Bacillus Coagulans HP-1 strain macerans KH91, microbial accession number KCTC 8734P).

< Comparative example  4. Bacillus Maseran's KH91 Using Bast fiber  Manufacturing>

The bast fiber was refined in the same manner as in Example 3, but the culture solution of Bacillus maserans KH91 of Comparative Example 3 was used instead of the culture solution of Bacillus coagulans HP-1 of Example 2.

< Comparative example  5. Using a refining solution that does not contain pupa extract Bast fiber  Manufacturing>

In the same manner as in Example 3, the bast fiber was refined, but 100 kg of water was added instead of 100 kg of pupa extract in the microbial nutrient solution during the first refining.

< Experimental Example  1.Check pectin resolution>

Bacterial strains of Bacillus coagulans HP-10, Enterobacter low gobi H. 2006, and Bacillus maserans KH91 were diluted in sterile water to 1 × 10 ⁶ / mL concentrations to contain 10 μg / mL of pectin (Nutrient Agar 10 μl each was inoculated on a 0.7 mm paper disc on the medium, followed by incubation at 50 ° C. for 2 days. After 2 days, the length of the transparent ring of the degraded agar medium was confirmed.

In the above method, the bacterium coagulans HP-10 of the present invention and enterobacter low-goal HIEL 2006 strain known as bast fiber refining strain, Bacillus maserans KH91, and the like cultures to determine the pectin resolution, as shown in Table 1 It was confirmed that the pectin resolution of the Bacillus coagulans HP-10 strain of the present invention is very excellent. The culture of the following strains was cultured by the method of Example 2, Comparative Example 1 and Comparative Example 3 and centrifuged to collect only strain pellets (pellets) was used.

microbe Diameter of agar medium transparent ring from which pectin is decomposed Bacillus coagullans HP-10 35 mm Enterobacter Jergovia H. 2006 15 mm  Bacillus maserans KH91 10 mm

< Experimental Example  2. Comparison of residuals of pectin and lignin>

The pectin and lignin residues of the bast fibers prepared by performing the refining steps of Example 3, Comparative Examples 2, 4 and 5 were investigated. The amount of pectin and lignin was used in the method commonly used in the art. Table 2 shows the analysis of the pectin and lignin components in hemp refined by each method.

Condition
Bast fiber
Pupa extract of secondary refinery Pectin
Relative Residue (%) Lignin
Relative Residue (%) Example 3 Bast Fiber Refined With Bacillus Coagulans HP-10
Included
4.5
4.2 Comparative Example 2 Bast fiber refined by Enterobacter J. gobbie 2006
Included
14.5
16.2 Comparative Example 4 Bast Fiber Refined With Bacillus Maserans KH91
Included
20.3
22.1 Comparative Example 5 Bast Fiber Refined With Bacillus Coagulans HP-10
Not included
10.2
12.3

In Table 2, the relative amounts of pectin and lignin are defined as 100 wt% of the pectin and lignin present in the hemp sample before being treated by the microorganism, respectively, and the pectin and the pectin present in the sample after the microorganism are treated. The content of lignin is shown as a relative value relative to 100% by weight. As can be seen from Table 2, it was confirmed that the degradation ability of pectin and lignin than Comparative Examples 2, 4 and 5 under the conditions of Example 3 using Bacillus coagulans HP-10 used in the present invention. . In other words, it was found that the refining effect of bast fiber was significantly higher in the case of refining using Bacillus coagulans HP-10, the case of adding pupa extract to the refining solution than other conditions.

< Experimental Example  3. Comparison of each fiber quality -1>

Next, the recovery and absorption of bast fibers of Example 3, Comparative Examples 2, 4 and 5 were compared to compare the quality of bast fibers under the respective conditions. The fiber recovery and absorbency were used in the art commonly used in the art. Table 3 is a table comparing the quality of bast fibers, respectively.

From the results in Table 3, the bast fiber of Example 3 of the present invention was found to have a higher absorbency than the bast fibers of Comparative Examples 2, 4 and 5, and the fiber recovery rate of the fiber refining process was superior. In other words, even when the fiber recovery rate and water absorption were confirmed, the refining effect of bast fiber was higher than that of the case of refining using Bacillus coagulans HP-10, the strain of the present invention, and adding pupa extract to the refining solution. It was found to be significantly higher.

Condition

Bast fiber
Pupa extract of secondary refinery
fiber
% Recovery
Absorbent
(%)
Example 3 Refined with Bacillus coagulans HP-10
Bast fiber
Included
97.4
16.2
Comparative Example 2 Bast fiber refined by Enterobacter J. gobbie 2006
Included
92.1
12.5
Comparative Example 4 Refined with Bacillus maserans KH91
Bast fiber
Included
88.2
8.2
Comparative Example 5 Refined with Bacillus coagulans HP-10
Bast fiber
Not included
94.4
14.2

< Experimental Example  4. Comparison of each fiber quality-2>

In order to check the quality of the bast fibers of Examples 3, Comparative Examples 2, 4 and 5, using the bast fibers to prepare a bedding (quilt, rug and pillow cover), the bedding each 10 days not to limit the age 50 men, women, young and old, which were not used, were subjected to preference under the following conditions. In other words, check the number of people checked in [○: the fiber material is very soft, △: the fiber material is slightly soft, ▲: the fiber material is slightly rough, and ×: the fiber material is too rough. The results for the acceptability of the fibers are shown.

Condition
Bast fiber
Pupa extract of secondary refinery
Likelihood ○ △ ▲ ×
Example 3 Bast Fiber Refined With Bacillus Coagulans HP-10
Included
47 people
3 people
0 people
0 people
Comparative Example 2 Bast fiber refined by Enterobacter J. gobbie 2006
Included

5 people
30 persons
14 people
1 person
Comparative Example 4
Bast Fiber Refined With Bacillus Maserans KH91
Included

1 person
13 people
35 persons
1 person
Comparative Example 5 Bast Fiber Refined With Bacillus Coagulans HP-10
Not included
10 people
35 persons
4 people
1 person ○: the fiber material is very soft, good, △: the fiber material is slightly soft
▲: The fiber material is slightly rough, ×: The fiber material is too rough

Referring to the results of Table 4, the palatability of the bedding prepared using the bast fiber of Example 3 of the present invention is significantly better than the bedding prepared using the bast fiber of Comparative Examples 2, 4 and 5 appear.

Claims (10)

(Step 1) After adding 250 parts by weight of the bast fiber raw material, 3,000 ~ 7,000 parts by weight of purified water and 80 ~ 120 parts by weight of 0.01% to 1% sodium carbonate aqueous solution and pretreatment for 5-6 hours at a temperature of 10 ~ 20 ℃;
(Step 2) Remove the liquid from the pre-treatment step, 3,000 ~ 7,000 parts by weight of purified water to 250 parts by weight of pretreated bast fiber raw material in one step, Bacillus Coagulans HP-10 ( Bacillus coagulans HP-10, Microorganism Accession No. KCTC10108BP) 90 to 110 parts by weight of microbial culture, 3 to 10 parts by weight of pectin as a microbial nutrient, 3 to 10 parts by weight of ammonium sulfate ((NH ₄ ) ₂ SO ₄ , yuan), sodium chloride (NaCl ) 3 to 10 parts by weight, 5 to 30 parts by weight of potassium II hydrogen phosphate, 90 to 110 parts by weight of pupa extract and 3 to 10 parts by weight of Tween 80, and the temperature of 40 to 55 ℃ and neutral pH Adjusting to primary refining for 20 to 40 hours; And,
(3rd step) Remove the liquid of the first refining step, and 0.01-1 to 5,000-15,000 parts by weight of the secondary refining purified water and the weight of the second refining purified water to 250 parts by weight of the first refined bast fiber raw material of the second step. 20 to 40 parts by weight of sodium bicarbonate, 20 to 40 parts by weight of 30% hydrogen peroxide, 20 to 40 parts by weight of sodium hydroxide and 20 to 40 parts by weight of sodium percarbonate are added by weight percent foaming agent and bleach. Second refining by washing for a minute;
Bacillus coagulans HP-10 ( Bacillus coagulans HP-10, microbial accession number KCTC10108BP) characterized in that it comprises a method of refining bast fibers. The method of claim 1,
The bast fiber raw material is Bacillus coagulans HP-10 ( Bacillus) characterized in that the one selected from the group consisting of flax, jute, jute and hemp coagulans HP-10, microbial accession number KCTC10108BP) method for refining bast fibers. The method of claim 1,
Bacillus coagulans HP-10 ( Bacillus), characterized in that the efficiency of refining by increasing the mechanical shaking in the first refining step of (2 step) coagulans HP-10, microbial accession number KCTC10108BP) method for refining bast fibers. The method of claim 1,
Bacillus coagulans HP-10 (microbial accession number KCTC10108BP), characterized in that the surfactant is added in an amount of 0.01 to 1% by weight of the weight of the purified water in the first refining step of (2 step) Method for refining bast fibers using The method of claim 1,
Bacillus coagulans HP-10 ( Bacillus coagulans HP-10, microorganisms) characterized in that after the secondary refining step of step (3), the bast fiber is separated, and the third refining step of refining by washing with clean water is performed. Method of refining bast fiber using Accession No. KCTC10108BP). A bast fiber refined using the method of any one of claims 1 to 5. Garment manufactured using the bast fibers of claim 6. Bedding article manufactured using the bast fibers of claim 6. An automotive interior product manufactured using the bast fiber of claim 6. A building interior product manufactured using the bast fiber of claim 6.

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