KR101171068B1 - Pharmaceutical Compositions for Diabetes Improvement and Prevention Containing Mineral Compositions as Effective Component - Google Patents

Abstract

The present invention relates to a pharmaceutical composition for improving and preventing diabetes and a health food containing the same, the mineral composition comprising the mineral composition as an active ingredient, and more specifically, sodium, calcium, magnesium, potassium, Pharmaceutical composition for improving and preventing diabetes and containing the mineral composition containing the mineral composition containing the mineral components of manganese, copper, zinc, lithium, strontium, germanium, selenium, vanadium, molybdenum and titanium as an active ingredient and the active ingredient It relates to a health food for improving and preventing diabetes. The pharmaceutical composition consisting of the mineral composition obtained from the marine mineral water is excellent in the metabolism of the cells, the effect of the osmotic pressure and body temperature control, the removal of free radicals in the body, especially the effect of blood sugar control and diabetes prevention, It can be used as a pharmaceutical composition or health food.

Description

Pharmaceutical Compositions for Diabetes Improvement and Prevention Containing Mineral Compositions as Effective Component

The present invention relates to a pharmaceutical composition for improving and preventing diabetes and a health food containing the same, and more particularly to a mineral composition obtained by drying or spray drying marine mineral water as an active ingredient. It relates to a pharmaceutical composition for improving and preventing diabetes and a health food containing the same.

Recently, with increasing interest in health, its functionality has attracted attention for various water resources. In particular, as the Law on Development and Management of Deep Sea Water was enacted in August 2007, research activities on physiological activities using not only freshwater resources but salt water resources have been conducted. It is underway (Yoo 2007; Jeong & Lee 2008).

In general, its efficacy is widely known, and along with deep seawater where research activities and commercialization have been carried out continuously, it is attracting attention as a brine resource, marine mineral water, one of the underground brine (Kim et al. 2008). Marine mineral waters have seawater in the deep ocean layers along the rock gaps or cracks in the deep rock layers of coastal or coastal areas, and the interaction of natural rock groundwater flowing down the deep underground aquifers with land and geological time and space conditions. It refers to storage, ripening and ionization (National Briefing 2007). Marine mineral water is richer in minerals such as Ca, Sr, Mn, Zn, Fe, Cu, Ni, V, and Se compared to deep ocean water, and the mineral balance of Ca and Mg is similar to human body fluids (Moon et al. 2004). . Among the minerals, Ca has been reported to improve the insulin metabolism and insulin resistance of cells upon oral ingestion. In addition, Mg has been reported to be capable of preventing and controlling hypertension, cardiovascular disease and diabetes.

Up to now, research on deep ocean water has been actively conducted. In the patent application for deep sea water, surface water is not suitable for food and beverage in the current state of deep sea pollution, but deep sea water is selected as a beverage because it does not contain much viable bacteria and contains little pathogenic organisms compared to the surface layer. In this case, the safety is very high (Japanese Patent Publication No. 7-34728). Such deep sea water is obtained by heating and concentrating seawater, cooling the concentrate, and then removing the crystals obtained from the concentrate, and continuing until the volume of the concentrate is concentrated to 1/1000 or less of the original volume. The enhancer and its manufacturing method are described, and a healthy drink effective for the prevention of modern diseases such as diabetes, allergic disease and myocardial infarction is disclosed by adding a part of the solution to the drink (Japanese Patent Publication No. 2580428). In addition, an electrolyte such as sodium chloride is added to the deep sea water to perform electrolysis in an electrolysis device, and the strongly acidic oxidation water (the redox potential is 1000 mV or more at pH 2.4 to 2.7 or more) obtained from the anode side and the alkali reduced water obtained from the cathode side are obtained. It is known that the use of strongly acidic oxidized water has been used for disinfection sterilization of medical instruments and sterilization of foods by its oxidative power and sterilization action by acid. Alkaline reduced water is known to be effective against gastrointestinal diseases such as abnormal fermentation in the stomach, chronic diarrhea, indigestion, antacids and excessive stomach acid (see Japanese Patent Laid-Open No. 2001-198575). In addition, as minerals contained in deep sea water, essential minerals such as metals such as copper and zinc, iodine, etc., in addition to calcium and magnesium, which have been known in the past, have been identified, and the salt water is desalted to reduce the content of sodium chloride. It has been known to develop vitamin nutrients by adding vitamins to the seawater (Japanese Patent Publication No. 60-255729), and since the number of viable bacteria is smaller than the surface water, this deep water is added to the drink with natural mineral water and developed as a mineral drink. (Japanese Patent Laid-Open No. 5-219921). Moreover, the beverage which added the bitter juice and mine obtained by taking sea water to the fresh water which was made into this fresh water using a reverse osmosis apparatus is known (Japanese Patent Laid-Open No. 10-150960).

However, these deep sea waters are excessively rich in sodium and chlorine compared to other minerals, and the deep sea water is about 1: 3, whereas the deep sea water is about 1: 3, which can burden the human body. There is this. Therefore, there is a problem that thirst when drinking deep sea water, stickiness is left when washing or bathing, and trace essential minerals such as Ge, Se, V are hardly reported.

The present inventors are trying to find an effective ingredient in seawater other than deep ocean water, Korea Research Institute for Chemical Testing, Gangwon-do Institute of Health and Environment, Korea Research Institute of Science and Technology, Korea Basic Science Institute, Gangneung Science Industry Development Institute, Japan Food Analysis Center, USA As a result of requesting analysis agencies such as ANRESCO, an inspection agency under the FDA, to analyze marine mineral water of Geumjin-ri, Okgye-myeon, Gangneung-si, Korea, the marine mineral water is more diverse than the deep ocean water, and minerals such as Ca, Mg, K, and Zn are abundant and detected in natural water. It was confirmed that a large amount of trace minerals, such as Se, V, which are not present.

Thus, the present inventors have tested the physiological activity and pharmacological effects of the mineral composition of the marine mineral water, the composition smoothes the metabolism of the cells, not only the effect of osmotic pressure and body temperature regulation, removal of free radicals in the body When the rats were used, the present invention was completed by confirming that the glycemic control and diabetes prevention effect is excellent.

It is an object of the present invention to provide a pharmaceutical composition and health food for diabetic improvement and prevention containing a mineral composition obtained by drying or spray-drying marine mineral water as an active ingredient.

In order to achieve the above object, the present invention is the sodium 8,500 ~ 9,100 mg, calcium 1,450 ~ 1,600 mg, magnesium 900 ~ 1,100 mg, potassium 200 ~ 225 mg, manganese 0.010 ~ 0.015 mg, 0.010 to 0.015 mg of copper, 8.00 to 9.00 mg of zinc, 0.080 to 0.09 mg of lithium, 25.0 to 35.0 mg of strontium, 0.001 to 0.002 mg of germanium (Ge), 0.250 to 0.300 mg of selenium (Se), 0.25 to vanadium (V) Provided is a pharmaceutical composition for improving and preventing diabetes, comprising a mineral composition comprising a mineral component of 0.30 mg, molybdenum 0.010 to 0.015 mg, and titanium 0.50 to 0.70 mg as an active ingredient.

The present invention also provides a health food for diabetic improvement and prevention containing the mineral composition as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present invention relates to a pharmaceutical composition for improving and preventing diabetes and health food containing a mineral composition using marine mineral water (hereinafter referred to as mineral water) taken from 1100 m underground near the Geumjin-ri coast, Gangneung-si, Gangneung-si, Gangwon-do, Korea. It is about. The present inventors studied the mineral water taken from the 1100 m underground near the Geumjin-ri coast, Ganggye-myeon, Gangneung-si, Gangwon-do, Korea, and found high mineral concentration, rich minerals, essential minerals and rare minerals, and the mineral composition of the mineral water. And it was confirmed that the mineral water containing this (水) is excellent in physiological activity efficacy.

The minerals of Jinjin marine mineral water of the present invention are rich in inorganic ingredients such as sodium, calcium, magnesium, potassium, and anti-cancer substances such as selenium and zinc, which facilitates the activation of metal ions necessary in the human body and promotes body absorption. In particular, selenium is a component of glutathione peroxidase enzyme, which inhibits the occurrence and metastasis of cancer, and is effective in hypertension, arteriosclerosis, cataract, etc. Germanium has a blood purification effect and decomposes and releases cholesterol attached to blood vessel walls. Function is known, vanadium is a mineral composition that shows good effects, such as cholesterol, cardiovascular and kidney disease.

 The present invention is sodium 8,500 ~ 9,100 mg, calcium 1,450 ~ 1600 mg, magnesium 900 ~ 1,100 mg, potassium 200 ~ 225 mg, manganese 0.010 ~ 0.015 mg, copper 0.010 ~ 0.015 mg, zinc obtained by drying or spray-drying 1 liter of marine mineral water 8.00-9.00 mg, lithium 0.080-0.09 mg, strontium 25.0-35.0 mg, germanium (Ge) 0.001-0.002 mg, selenium (Se) 0.250-0.300 mg, vanadium (V) 0.25-0.30 mg, molybdenum 0.010-0.015 Provided is a pharmaceutical composition for improving and preventing diabetes, comprising a mineral composition comprising a mineral component of mg and titanium 0.50 to 0.70 mg as an active ingredient.

In the pharmaceutical composition for treating and preventing diabetes of the present invention, the pharmaceutical composition preferably contains 0.5 to 5% by weight of the mineral composition, and is preferably administered at 0.1 to 1 g per kg of body weight. In addition, in the pharmaceutical composition, the marine mineral water is more preferably withdrawn from the point of 1000 ~ 1200 m sea water of Geumjin-ri, Okgye-myeon, Gangneung-si, Gangwon-do, South Korea, the ratio of calcium and magnesium concentration in the mineral composition is 1.6: It is preferable that it is 1.

Pharmaceutical compositions containing the mineral composition of marine mineral water of the present invention as an active ingredient can be administered orally or parenterally during clinical administration and can be used in the form of general pharmaceutical preparations.

That is, the composition of the present invention can be administered in various oral and parenteral dosage forms in actual clinical administration, and when formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, surfactants, etc., which are commonly used Is prepared using. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid preparations include at least one excipient such as starch, calcium carbonate, sucrose in the mineral composition. Or lactose, gelatin, etc. In addition to simple excipients, lubricants such as magnesium styrate talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. Propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used as the non-aqueous solvent and suspension agent. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like. In addition, calcium or vitamin D ₃ may be added to improve the efficacy as a prophylactic or therapeutic agent for osteoporosis.

 Dosage units may contain, for example, one, two, three or four times, or 1/2, 1/3 or 1/4 times the individual dosage. Individual dosages preferably contain an amount in which the effective drug is administered at one time, which usually corresponds to a full, 1/2, 1/3 or 1/4 times the daily dose.

An effective dose of the composition may be administered in an amount of 0.01 to 10 g, preferably 0.1 to 1 g, 1 to 2-3 times per kg of body weight. Such a composition is not necessarily limited thereto, and may vary according to the condition, weight, age, sex, health condition, diet, drinking method, excretion rate, and severity of symptoms. It does not limit the scope of the invention.

The present invention also provides a health food for diabetic improvement and prevention containing the mineral composition as an active ingredient.

In the health food of the present invention, the mineral composition is preferably included in 0.1 to 5.0% by weight relative to the total weight of the health food.

When the mineral composition of the present invention is used as a food additive, the mineral composition may be added as it is or used with other food or food ingredients, and may be appropriately used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to its intended use (prevention, health or therapeutic treatment). Generally, in the preparation of food or beverages, the mineral composition of the present invention is added in an amount of 0.1 to 15% by weight, preferably 0.2 to 10% by weight relative to the raw material. However, in the case of long-term intake for health and hygiene or health control, the amount may be below the above range, and the active ingredient may be used in an amount above the above range because there is no problem in terms of safety. Is sure.

There is no particular limitation on the kind of food. Examples of the food to which the substance can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, ice cream, various soups, drinks, tea, drinks, Alcoholic beverages and vitamin complexes, and the like and include all of the health foods in the conventional sense.

Minerals, unlike proteins, sugars and lipids, cannot produce energy, and unlike vitamins, they are not destroyed during cooking. However, since it dissolves in water, discarding the solution during cooking results in loss. Compared to the important functions of many minerals, the actual amount present is very small. However, the absence of any of these elements in any quantity can cause serious problems and, in the case of excess, can have a toxic effect. Minerals are classified into essential minerals (large amounts, trace elements), semi-essential minerals, non-essential minerals, and poisonous minerals, depending on the function of the body.In the essential minerals, Ca, Cl, Mg, It is divided into a large element such as P, K, Na, and S, and a small element such as Fe, Zn, Se, Mn, Cu, I, Mo, Co, Cr, and F as elements having a daily requirement of 0.01 g or less.

In view of the deficiency and efficacy of essential minerals, first, Cu is an essential trace element present in the body as part of enzymes and other proteins, which transports stored iron and intestinal iron to hemoglobin production sites. In addition, it is involved in the production of ATP (adenosine triphosphate) by acting in the final process of electron transport system in the mitochondria, and also contributes to maintaining the connective tissues of skeletal and cardiac circulation normal, and the antioxidant enzyme SOD (Superoxide) alone or in combination with zinc. Dismutase) also prevents oxidative damage to cells. In deficiency, anemia, leukocyte reduction, and neutrophil reduction occur, and as an effect, cardiovascular disease prevention, arthritis treatment, antioxidant function, and anti-inflammatory effect can be seen, and the daily recommended amount is 1.5 to 3.0 mg / day.

Sodium (Na) is an important component of extracellular fluid, along with chlorine and bicarbonate ions, plays an important role in maintaining the acid-base equilibrium of the body, maintaining the osmotic pressure of body fluids, and determining the amount of fluid. It causes cramps, weight loss, headaches, and insomnia. Its effects include maintaining and supplying normal fluids, maintaining normal acid balance of the human body, maintaining normal muscle reactions, or maintaining normal osmotic pressure. The recommended daily dose is 10 g or less. Koreans usually consume 10 to 30 g.

Magnesium (Mg) is present in the body of about 20 to 28 g, about 60% in bone, 26% in muscle, the remainder in soft tissues and body fluids, an important component of intracellular fluid, kidney, such as kidney stones in the absence of Diseases, hyperthyroidism, acute alcoholism, parathyroid disorders, muscle spasms, and the like.Efficacy includes: carbohydrate metabolism, lipid metabolism, synthesis of protein metabolism and nucleic acid, synthesis of phospholipids (lecithin) in the body, vitamin C Can assist with the metabolism of B complex, phosphorus, sodium, potassium and calcium, and the excitatory calming effect of the nervous system. The recommended daily dose is 300 mg.

Manganese (Mn) contributes to the activation of various enzymes, and deficiency causes diabetes symptoms due to weight loss, dermatitis, nausea or vomiting, discoloration of hair, decreased fertility or reduced pancreatic function. The treatment effect of psychotic symptoms such as mood swings or schizophrenia, sexual enhancement, diabetes and asthma treatment effect can be seen, the daily recommended amount is 2.5 to 5 mg.

Selenium (Se) has 1,970 times the antioxidant power of natural tocopherol, and deficiency causes cancer, heart disease, decreased immune function, red blood cell function, muscle pain, myocardial decline, muscle tone, and pancreatic function decline. Benefits include detoxification of heavy metals, antioxidant activity, anti-aging, anti-cancer, prevention of cardiovascular disease, prevention of liver cirrhosis, prevention of inflammatory diseases, prevention of complications of diabetes, enhancement of liver and kidney function, enhancement of reproductive function, prevention of cataracts, improvement of immune function, etc. The effect of can be seen, the recommended daily amount is 50 to 200 μg.

Zinc (Zn) is found in all somatic cells and is a constituent of more than 200 enzymes that, when deficient, cause fetal malformations, sleep and behavioral disorders, acne or dermatitis, alcoholic cirrhosis, inflammatory bowel disease, autism or psychosis. Induced by the effect, such as promoting fetal growth, sperm formation, strengthen skin resistance and dermatitis treatment, promote growth and development, increase insulin secretion, prevent atherosclerosis or regulate thyroid function, the daily recommended amount is 15 Mg.

Phosphorus (P) is the second most abundant mineral in the body after calcium. It is responsible for the storage and release of energy, is a component of phospholipids and lipoproteins, and plays an important role in the structure and function of cells. It also acts as a coenzyme for nucleic acids. In case of deficiency, it becomes lethargic due to a decrease in ATP synthesis, induces weight loss in children, rickets disease, osteomalacia in adults, and as an effect, combines with calcium to prevent rickets and osteomalacia, and combines with many vitamin B to have enzymatic activity, 1 The recommended daily dose is 800 mg.

Potassium (K) is the most important mineral for acidic alkali equilibrium of intracellular fluids with an adult total body weight of 150 g, and deficiency causes arrhythmia, hypoglycemia, asthenia, etc. Normal acid balance, maintain normal muscle reaction, maintain normal osmotic pressure, vasodilation, promote insulin secretion, defense effect of myocardial infarction, the recommended daily dose is 3,600 to 10,000 mg.

Calcium (Ca) is the most abundant element in the body's minerals, and about 700 mg of calcium moves between bone and blood every day. In case of deficiency, the calcium in the blood decreases peripheral calcium, muscle junction bones and bloodiness, and the mild stimulation causes tetanosis, which causes muscles, mainly the hands, feet, and facial muscles to contract and cramp, and the effects include osteoporosis, rickets, Treatment of fractures, caries, degenerative degenerative arthrosis, prevention of hypertension, arteriosclerosis, diarrhea or diabetes, prevention of allergic or cold, insomnia, calcium hypersensitivity, increase myocardial contractility, calcium and muscle bone and blood, blood coagulation The promoting effect of can be seen, the recommended daily amount is 1 to 1.2 g.

Iron (Fe) is contained in hemoglobin and plays an important role in binding to oxygen. When iron is insufficient, hemoglobin cannot be produced, causing red blood cells to become smaller than normal, making complexion worse, fatigue easily, and balancing iron. Loss leads to anemia.

Therefore, it is intended to develop a natural mineral health beverage composition for easily ingesting essential trace mineral components not obtained in general food and beverages, and to balance the minerals of the body.

Seawater contains a large amount of magnesium, potassium, silicon, etc. in addition to sodium chloride, but rare minerals are rarely reported.

As described above, the mineral composition of the present invention contains abundant natural minerals and rare minerals to clear the blood, facilitate the metabolism of the cells, and the effect of osmotic pressure, body temperature control, removal of free radicals in the body is excellent In addition, pharmaceutical compositions and health foods containing the composition as an active ingredient are excellent in preventing glycemic control diabetes.

1 is a spray drying process of mineral water and mineral water prepared by the above process,
2 is a mineral water production process by mixing the mineral water mineral composition and purified water and ultrafine filtration of the mineral water,
Figure 3 is a graph showing the change in body weight of the mice administered the pharmaceutical composition containing mineral water of the present invention,
Figure 4 is a graph showing the difference in the abdominal fat of mice to which the pharmaceutical composition containing mineral water of the present invention,
5 is a graph showing differences in testicular fat of mice to which the pharmaceutical composition containing mineral water of the present invention is administered,
Figure 6 is a graph showing the difference in dietary efficiency of the mice administered with the pharmaceutical composition containing mineral water of the present invention,
7 is a graph showing changes in blood glucose levels of mice to which the pharmaceutical composition containing mineral water of the present invention is administered,
8 is a graph showing the difference in blood insulin concentrations of the mice administered the pharmaceutical composition containing mineral water of the present invention,
9 is a graph showing the difference in HOMA-IR of the mouse to which the pharmaceutical composition containing mineral water of the present invention is administered,
10 is a graph showing GLUT4 expression in the extensor extensor muscles of the mice to which the pharmaceutical composition containing mineral water of the present invention was administered,
Figure 11 is a graph showing the GLUT4 expression in the soles of mice administered with a pharmaceutical composition containing mineral water of the present invention.

Hereinafter, the present invention will be described in more detail based on the following examples. It should be noted, however, that the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention. The present invention is not limited to the following examples. Will be apparent to those skilled in the art to which the present invention pertains.

< Example  1> Preparation of Mineral Composition

Mineral water was extracted by digging up the rock where mineral water was standing, and after filtering the mineral water with a filter of 1.0 μm, the filtered mineral water was spray-dried (Spray Dryer, L-8, Japan) (see FIGS. 1 and 2). ) Into the raw material tank. At this time, the mineral water is supplied to the atomizer of the spray drying apparatus from the raw material tank by operating the pump. The mineral water supplied to the atomizer was sprayed with fine particles by falling to the surface of the rotating disk at a speed of 12,000 RPM, and at the same time as the spraying process, by applying hot air of 180 ° C supplied from the surrounding windbox of the atomizer, the water was evaporated. A dry powdered mineral composition (see FIGS. 1 and 2) was prepared.

< Comparative example  1> sea water, Deep ocean water Analysis of minerals in mineral waters

Minerals of mineral water taken from 1100 m underground near the coast of Geumjin-ri, Gangneung-si, Gangneung-si, Gangwon-do, and minerals of seawater and deep seawater are shown in Table 1 and Table 2 below. These ingredients were compared to the Korea Institute of Science and Technology (KIST), the Gangneung Science Industry Promotion Institute, the Korea Chemical Testing Institute, the Gangwon-do Institute of Health and Environment, the Japan Food Analysis Center, and the ANRESCO Analysis Institute in the United States.

Massive mineral analysis of seawater, deep seawater and mineral water Analysis item Geumjin Mineral Water (mg / ℓ) Seawater (mg / ℓ) Deep sea water (mg / l) Sodium (Na) 9070 10,500 10,767 Calcium (Ca) 1,600 401 414 Magnesium (Mg) 1,100 1,300 1,320 Potassium (K) 224 380 410

Geumjin mineral water in Gangneung was similar in salt concentration to seawater and deep sea water, but showed a big difference in mineral composition. There was no significant difference in mineral composition between seawater and deep sea water.

To explain this in more detail, calcium and magnesium have been reported to reduce blood pressure and prevent myocardial infarction when ingested at appropriate concentrations, and the sweet calcium concentration was very high compared to seawater, especially calcium and magnesium. The ratio of concentration maintained the ratio (1.6: 1) which is known to be ideally absorbed by the human body (Table 1).

However, these seawater and deep sea waters have a high content of sodium and chlorine compared to other minerals, and the Jinjin mineral water in Gangneung has a ratio of 1.6: 1 that is known to be ideal for absorption of calcium and magnesium concentrations. There is a problem in that the deep ocean water to be compared is a burden on the human body at a ratio of about 1: 3. Therefore, there is a problem that thirst when drinking deep sea water, stickiness is left when washing or bathing.

Trace mineral analysis of seawater, deep seawater and mineral water Analysis item Geumjin Mineral Water (mg / ℓ) Seawater (mg / ℓ) Deep sea water (mg / l) Iron (Fe) 23 0.01 0.023 Manganese (Mn) 0.011 0.002 0.002 Copper (Cu) 0.012 0.0006 0.0004 Zinc (Zn) 8.46 0.01 0.009 Li (Li) 0.089 ND ND Strontium (St) 30.4 8 7.54 Germanium (Ge) 0.0014 ND 0.0005 Selenium (Se) 0.302 ND ND Vanadium (V) 0.305 ND ND Molybdenum (Mo) 0.011 ND 0.01 Titanium (Ti) 0.62 ND ND

ND: Not Detected

Geumjin mineral water in Gangneung was found to contain trace minerals in the analysis of trace minerals compared to seawater and deep sea water, and rare minerals were found to be higher in Geumjin mineral water as compared to seawater and deep sea water. ). Among them, selenium is a component of glutathione peroxidase enzyme, which inhibits the occurrence and metastasis of cancer, and is effective in hypertension, arteriosclerosis, cataract, etc. Germanium has a blood purification effect and decomposes and releases cholesterol attached to the wall of blood vessels. Function is known, vanadium is associated with cholesterol, cardiovascular and kidney disease.

The unusual mineral content and composition of Geumjin mineral waters may affect the results of this study. Salt concentrations are more diverse than similar deep sea water, and minerals such as calcium, magnesium, potassium, and zinc are abundant, and rare minerals such as selenium (Se) and vanadium (V), which are not detected in general water, existed.

Thus, the mineral composition is obtained by taking 1 liter of Jinjin mineral water and drying or spray drying, sodium 8,500-9,100 mg, calcium 1,450-1,600 mg, magnesium 900-1,100 mg, potassium 200-225 mg, manganese 0.010-0.015 mg, 0.010 to 0.015 mg of copper, 8.00 to 9.00 mg of zinc, 0.080 to 0.09 mg of lithium, 25.0 to 35.0 mg of strontium, 0.001 to 0.002 mg of germanium (Ge), 0.250 to 0.300 mg of selenium (Se), 0.25 to vanadium (V) 0.305 mg, molybdenum 0.010 ~ 0.015 mg, titanium 0.50 ~ 0.70 mg, the ratio of calcium and magnesium concentration is 1.6: 1 by weight ratio.

< Example  2> Preparation of Mineral Water Using Mineral Composition

100 L of purified water passed through the ultra-fine filter was added to the mixing tank, and 3 kg of the mineral composition obtained in Example 1 was added to the mixing tank and mixed. The mixture was sterilized at 90 ° C. for 10 minutes, and then filtered with a 1.0 μm filter to prepare mineral water including a mineral composition.

< Example  3> Preparation of Sample

The mineral water was centrifuged at 3,000 rpm for 5 minutes, and the upper layer was recovered. Then, DMEM (Dulbecco's MEM) powder and NaHCO ₃ were added thereto, adjusted to pH 7, and filtered through a 200 nm pore size filter. A certain amount added was used as a sample.

The present inventors experimented through scientifically validated data to verify the effect of mineral water on the anti-diabetic effect of the human body, and as a result proved the effect on the anti-diabetic effect. This will be described below.

< Example  4> Using mineral composition Anti-diabetic  Test

The present inventors pretreated the blood components and muscle tissues after diet preparation and animal breeding, and performed biochemical analysis of the muscle tissues. All experimental results are expressed as mean ± SEM. One-way ANOVA was used to verify the significance between the groups. Scheffe's post-test was performed for items with significant differences. Statistically significant difference was set at p <0.05. Hereinafter, look in detail as follows.

<4-1> Laboratory Animals and Breeding

Sprague-Dawley rats were used to induce high fat diet and to induce insulin resistance and type 2 diabetic rats. This test was conducted on 6-week-old male Sprague Dawley for 4 weeks in the control diet sedentary group (CS group), the high fat diet sedentary group (HS group), and the high-fat diet beverage group. (HT group: high fat diet test drink supplemented group) to breed. In order to adapt to the environment for 1 week, pre-breeding was carried out for 1 week before the test. The room temperature was 23 ± 1 ℃ and the humidity was 50 ± 5%. During the breeding period, 20: 00-08 Set to: 00. During the breeding period, diet and water allowed free intake.

<4-2> Dietary composition

AIN-76 was used as a general diet and high fat diet was prepared based on AIN-76. Fat was replaced with lard instead of corn oil to increase fat content to 35% (Table 3).

Composition of Experimental Diet (g / kg Diet) Control diet High fat diet Casein 200 200 Starch 500 200 Sucrose 150 150 Corn oil 50 - Lard - 350 Cellulose 50 50 Vitamin mix 10 10 Mineral mix 35 35 DL-methionine 3 3 Choline barbiturate 2 2 DL-∝-tocopherol 1.2 1.2 Total calories 3,850 yen 5,350 yen

<4-3> Preparation and Administration of Pharmaceutical Composition

After preparing the mineral water mineral composition prepared in Example 1 in powder form at a ratio of 2.8 g / 100 ml using distilled water, 1 ml of the composition per kg of body weight was orally administered at a predetermined time every day, and the HS group had the same stress. The same amount of water was orally administered in order to load.

<4-4> Biochemical Analysis

<4-4-1> Body weight and Dietary  Measure

Dietary intake during the breeding period was measured once a day, body weight once a week at a constant time. The feed efficiency ratio (FER) is expressed as the total weight intake divided by the weight gain during the experiment.

Analysis of changes in body weight (FIG. 3), testicular fat (FIG. 4), abdominal fat (FIG. 5), and dietary efficiency (FIG. 6) to determine the changes in body weight and form after 4 weeks of swimming exercise and composition loading. It was. Body weight did not change significantly between groups after 4 weeks of breeding, but dietary fat growth rate was significantly higher in the high fat diet control group (HS) than in the normal diet control group (CS). However, there was no effect of fat reduction by deep water. On the other hand, dietary efficiency was significantly lower in CS group than in HS group and high-fat beverage test group (HT).

<4-4-2> blood analysis

After 4 weeks of breeding, anesthesia was performed using ether after 18 hours of fasting, blood was isolated through the abdominal vena cava, and the testicular fat, abdominal fat and skeletal muscle of the lower limb were extracted. In order to analyze blood components, blood was centrifuged at 700 x g for 15 minutes to separate serum. All samples were stored in cryogenic freezers at −70 ° C. until analysis. Blood glucose levels were analyzed by enzyme method using a commercial kit using a spectrophotometer (UV-MINI 1240, Shimadzu, Japan). In addition, blood insulin concentration was measured using RAT INSULIN RIA KIT (Linco Research, Inc. St. Louis MO), and was read by γ-counter (1470 Wizard, Wallac, Automatic Count, Finland).

As a result of analyzing the biochemical changes after 4 weeks of deep water loading, the CS group and the HT group were significantly lower than the HS group in the change of blood glucose, suggesting the hypoglycemic action by the composition of the present invention (FIG. 7). ). In addition, in the change of blood insulin concentration, the HT group was lower than the HS group and the CS group (FIG. 8). In addition, in the HOMA-IR indicating a measure of insulin resistance, the HT group was lower than the HS group and the CS group, indicating that ingestion of the composition of the present invention improved insulin resistance in rats (FIG. 9).

Morphologically, obese patients were more likely to develop type 2 diabetes, but in the HS group who consumed high-fat diet, the weight of abdominal fat and testicular fat was ingested by the high-fat diet. Significantly increased compared to the CS group, but the same high-fat diet was ingested, but the HT group ingesting the composition of the present invention showed a relatively decreased fat weight, and thus obesity was resolved, indicating that it was effective for type 2 diabetes.

Fasting blood glucose levels were significantly decreased in the HT group compared to the HS group, and thus decreased to the level of the general diet group. In HOMA-IR, which shows the degree of insulin resistance, the HT group was the lowest, indicating that it was effective in the hypoglycemic action of hot spring water and type 2 diabetes.

<4-4-3> of muscle GLUT4  Protein expression

Pretreatment of muscle was homogenized by placing the muscle sample in 1 ml of buffer (20 mM Tris-Hcl, 5 mM EDTA, 2 mM PMSF, 0.01 mg / ml of aprotinin, 0.01 mg / ml of leupeptin, 0.01 mg / ml of pepstatin A, pH 7.5). Work was carried out. After homogenization, the muscle sample was centrifuged at 1,200 × g for 10 minutes at 4 ° C, the supernatant was separated, and then the supernatant was centrifuged at 9,000 × g for 10 minutes, and then the supernatant was removed from the BCA protein assay kit (Bio-Rad, USA). ) Was used to quantify the protein and then to measure GLUT4 protein. Meanwhile, for protein analysis of GLUT4, 20 ㎍ and 40 ㎍ of EDL and Soleus protein samples were loaded on 10% SDS-PAGE gel, respectively, and electrophoresed to separate proteins. After protein separation, transfer to PVDF membrane, followed by blocking for 1 hour and 30 minutes using Blocking buffer (5% skim milk in PBS). After diluting with primary antibody GLUT4 (santa Cruz, U.S.A.) 1: 500 and reacting for 2 hours, it was washed 5 times with PBS (5% tween-20) for 10 minutes. The rabbit anti-goat IgG (santa Cruz, USA), a secondary antibody, was diluted 1: 2,000 and reacted for 1 hour, and then washed 5 times with PBS (5% tween-20) for 10 minutes. Identification of the band was performed on the X-ray film after emitting the membrane by using the ECL kit. The density of the band was analyzed by densitometry (Bio-Rad, U.S.A.).

In the expression level of GLUT4 by muscle type, HT group was higher in EDL than in CS group and HS group, but there was no difference between groups in Soleus. Expression of GLUT4 lowered by the high fat diet (HS group) was as high as CS group by the ingestion of the composition of the present invention (Figs. 10 and 11).

In the expression level of GLUT4, a protein that absorbs blood glucose in muscle, the HT group was the highest in the intestinal extensor muscle (EDL), and the HT group was recovered as much as the CS group in the soleus, the root muscle. These results are evidence showing that the effect of lowered blood glucose levels and insulin sensitivity by the composition of the present invention effectively worked in muscle.

< Manufacturing example  1>

The pharmaceutical composition of the present invention can be administered in the following formulations, and the following formulation examples are merely illustrative of the present invention, whereby the content of the present invention is not limited.

<1-1> Preparation of Injectables

40 mg of marine mineral water dry powder of Geumjin-ri, Okgye-myeon, Gangneung-si, Gangwon-do, Korea

Sodium Metabisulfite 3.0 mg

Methylparaben 0.8 mg

Propylparaben 0.1 mg

Appropriate sterile distilled water for injection

The above ingredients are mixed and prepared in a conventional manner to have a final volume of 2 ml, followed by filling into a 2 ml ampoule and sterilizing to prepare an injection.

<1-2> Manufacture of tablets

80 mg of dried marine mineral water of Geumjin-ri, Okgye-myeon, Gangneung-si, Gangwon-do, Korea

Lactose 100 mg

Starch 100 mg

Magnesium stearate proper amount

A tablet is prepared by mixing and tableting the above components according to a conventional tablet manufacturing method.

<1-3> Preparation of capsules

40 mg of marine mineral water dry powder of Geumjin-ri, Okgye-myeon, Gangneung-si, Gangwon-do, Korea

Lactose 50 mg

Starch 50 mg

Talc 2 mg

Magnesium stearate appropriate amount

The above components are mixed according to a conventional capsule preparation method and filled in gelatin capsules to prepare capsules.

<1-4> Liquid  Produce

400 mg of marine mineral water dry powder of Geumjin-ri, Okgye-myeon, Gangneung-si, Gangwon-do, Korea

20 g of sugar

20 g of isomerized sugar

Lemon incense quantity

Purified water was added to adjust the total volume to 1000 ml. According to the conventional method for preparing a liquid, the above components are mixed, and then filled into a brown bottle and sterilized to prepare a liquid.

< Manufacturing example  2> Preparation of health food containing mineral composition as active ingredient

The present inventors have confirmed that the anti-cancer, diabetic prevention and therapeutic activity of the mineral composition of the marine mineral water powder through the above embodiment was excellent, and prepared a health food containing the same as an active ingredient as follows.

<2-1> Preparation of Drink

522 mg of honey

Chioctosanamide 5 mg

Nicotinamide 10 mg

Riboflavin Sodium Hydrochloride 3 mg

Pyridoxine hydrochloride 2 mg

Inositol 30 mg

Orthoic acid 50 mg

100 to 150 mg of mineral composition in powder of mineral water

200 ml of water

A beverage was prepared using the above-mentioned composition and content by a conventional method.

<2-2> Of chewing gum  Produce

Gum base 20%

Sugar 76.36-76.76%

3-4% mineral composition from mineral water powder

1% fruit flavor

Water 2%

Chewing gum was prepared using the above-mentioned composition and content by a conventional method.

<2-3> Preparation of Candy

Sugar 50-60%

Starch syrup 39.26 ~ 49.66%

Mineral composition in powder of mineral water 2-3%

Orange flavor 0.1%

The composition and the content of the candy were prepared using a conventional method.

<2-4> Biscuit  Produce

Force Class 1 88 kg

Gravity First Class 76.4 ㎏

16.5 kg per white

2.5 kg of salt

2.7 kg of glucose

Palm shortening 40.5 kg

5.3 kg of ammo

Medium kg 0.6 kg

0.55 kg sodium bisulfite

Rice flour 5.0 kg

Vitamin B1 0.003 kg

0.003 kg of vitamin B2

Milk Flavor 0.16 ㎏

71.1 kg of water

Whole milk powder 4 kg

Substitute powder 1 kg

0.1 kg of calcium phosphate

Spray salt 1 kg

25 kg of spray oil

Mineral composition of powder of mineral water 2-5 kg

Biscuits were prepared using conventional methods with the above composition and content.

<2-5> Preparation of Ice Cream

Milkfat 10.0%

Non-fat solids 10.8%

Sugar 12.0%

Starch syrup 3.0%

Emulsifying stabilizer (span) 0.5%

Spices (Strawberries) 0.15%

61.31% to 60.91% water

Mineral composition from powder of mineral water 2.4-3.4%

Ice cream was prepared using conventional methods using the above composition and content.

<2-6> Chocolate  Produce

Sugar 34.36-34.76%

Cocoa Butter 34%

Cocoa Mass 15%

Cocoa Powder 15%

Lecithin 0.5%

0.5% vanilla

Mineral composition from powder of mineral water 2.4-3.4%

With the above composition and content, chocolate was prepared using conventional methods.

Although the composition ratio is mixed composition of a relatively suitable component in a preferred embodiment, the composition ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, use purpose.

Although the present invention has been described in detail only with respect to the described embodiments, it will be apparent to those skilled in the art that various modifications and variations are possible within the technical scope of the present invention, and such modifications and variations belong to the appended claims.

Claims (7)

Obtained by drying or spray-drying 1 liter of marine mineral water
Sodium 8,500 ~ 9,100 mg, Calcium 1,450 ~ 1,600 mg, Magnesium 900 ~ 1,100 mg, Potassium 200 ~ 225 mg, Manganese 0.010 ~ 0.015 mg, Copper 0.010 ~ 0.015 mg, Zinc 8.00 ~ 9.00 mg, Lithium 0.080 ~ 0.09 mg, Stron Diabetes containing a mineral composition comprising a mineral composition of 25.0 to 35.0 mg of cerium, 0.001 to 0.002 mg of germanium, 0.250 to 0.300 mg of selenium, 0.250 to 0.350 mg of vanadium, 0.010 to 0.015 mg of molybdenum, and 0.50 to 0.70 mg of titanium. Pharmaceutical compositions for improvement and prevention.
The pharmaceutical composition of claim 1, wherein the pharmaceutical composition contains 0.5 to 5% by weight of a mineral composition.
The pharmaceutical composition for improving and preventing diabetes of claim 1, wherein the pharmaceutical composition is administered at 0.1 to 1 g per 1 kg of body weight.
According to claim 1, wherein the ratio of the calcium and magnesium concentration in the mineral composition is a weight ratio of 1.6: 1, characterized in that for improving and preventing diabetes pharmaceutical composition.
The method of claim 1, wherein the mineral mineral water is a diabetic improvement and prevention pharmaceutical composition, characterized in that is taken from the sea water 1000 ~ 1200 m point of Geumjin-ri, Okgye-myeon, Gangneung-si, Gangwon-do, South Korea.
A health food for diabetic improvement and prevention containing the mineral composition of any one of claims 1 to 5 as an active ingredient.
The method of claim 6, wherein the mineral composition is diabetic improvement and prevention health food, characterized in that it comprises 0.1 to 5.0% by weight relative to the total weight of the health food.

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