KR101139686B1 - A cosmetic composition comprising bittern - Google Patents

Abstract

The present invention relates to a cosmetic composition for anti-wrinkle comprising a liver water as an active ingredient. Specifically, since the cosmetic composition comprising the water of the present invention as an active ingredient has excellent collagen generating activity, antioxidant effect and excellent moisturizing effect, not only the wrinkle improvement effect and the anti-aging effect are recognized, but also the natural substance as an active ingredient As a problem of side effects does not occur, it can be widely used as a cosmetic composition.

Guard, wrinkle prevention, collagen

Description

Cosmetic composition containing a guard water {A COSMETIC COMPOSITION COMPRISING BITTERN}

The present invention relates to a cosmetic composition having an anti-wrinkle effect, an anti-aging effect and a moisturizing effect.

In the past, Chinese products, which were thought to be used by women, are used not only as a beauty expression tool but also as household goods for men and women of all ages. Increasingly, there is a growing interest in 'functional' products that expect a particular effect or efficacy.

The functional cosmetics currently recognized in Korea are whitening products that help to whiten the skin, wrinkle improvement products that help to improve the wrinkles of the skin, and sunscreen products that burn the skin finely or protect the skin from UV rays. have. In general, such functional cosmetics are interpreted as cosmetics that affect the structure or function of the body as well as cosmetic effects, and prevent and treat. In particular, as the sales proportion of functional cosmetics has increased considerably in the total sales of cosmetics, researches to find new efficacy ingredients on the human body and use them effectively on the skin have been actively conducted.

Recent researches on functional materials have been focused on natural materials derived from natural products in conjunction with environmentally friendly hot air, and researches on substances used in herbs and herbal medicines have been conducted.

On the other hand, in recent years, as a natural salt is recognized as a food, interest in the natural salt, aging salt of the natural salt and the processed salt of the natural salt, and the brine generated in the manufacturing process of the natural salt is increasing. According to such interest, the necessity of the study about not only using a natural salt, a brine, etc. as a food, but also using it as an additive or an active ingredient of the external composition containing a therapeutic composition, a cosmetic composition, etc. is increasing. In particular, the three sides of the sea, considering the geographical environment of Korea, which is rich in marine resources, the need for research related to the identification and development of the functionality, such as natural salts and water is increasing.

The present invention is to improve the problems of the prior art as described above, an object of the present invention to provide a cosmetic composition comprising a natural substance as an active ingredient.

In order to achieve the above object, the present invention provides a cosmetic composition comprising a liver water as an active ingredient.

The inventors of the present invention, while studying the functionality of the liver derived from natural substances, as a result of cell migration rate measurement and collagen production results confirmed that the liver water as compared to the sun salt and purified salt, especially anti-wrinkle effect, excellent moisturizing effect, The present invention was completed by confirming that it is not a problem for side effects or resistance because it is prepared from natural substances that can be used as food additives.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a cosmetic composition comprising the liver water as an active ingredient.

In the present invention, bittern is a mother liquor remaining when salt, ie, sun salt, is precipitated in seawater. Generally, it can be obtained in the process of preparing salt or by using deliquescent action in the process of storing salt.

In one embodiment, the brine of the present invention may be prepared by a method of dehydration by centrifugation at 1000 to 10,000 rpm and 10 to 25 ℃, preferably 5,000 to 7,000 rpm and 10 to 20 ℃ the natural salt, The brine may further be a brine dried product in which the dehydration step and the spray drying step are performed.

For example, the brine may be obtained from seawater or sun salt collected on the west coast of Korea, specifically, the Jeollanam-do coastal area, and preferably, the lithium (Li) content is 0.0035 to 0.0045 wt% based on the total weight of the brine, Magnesium (Mg) content is 20.1 to 20.2% by weight, potassium (K) content is 4.1 to 4.2% by weight, manganese (Mn) content may be 0.00165 to 0.00175% by weight, more preferably lithium (Li) content This may be 0.004 to 0.0044% by weight, magnesium (Mg) content of 20.14 to 20.16% by weight, potassium (K) content of 4.14 to 4.16% by weight, and manganese (Mn) content of 0.0017 to 0.00173% by weight.

Natural salt in the present invention refers to the raw salt made by concentrating the seawater by evaporating water using natural forces such as wind and sunlight after introducing the seawater or seawater into the reservoir or salt field, and the crystals of coarse and semi-transparent hexagonal Has a characteristic. The sun salt is called mineral salt because it is rich in many minerals (minerals) such as calcium, magnesium, zinc, potassium, and iron.

The sun salt is, for example, based on the total weight of the sun salt, sodium chloride content of 75 to 95% by weight, calcium content of 0.1 to 0.3% by weight, potassium content of 0.15 to 0.45% by weight, magnesium content of 0.4 to 2.0% by weight Or sodium chloride content of 80 to 90% by weight, calcium content of 0.11 to 0.22% by weight, potassium content of 0.2 to 0.35% by weight, magnesium content of 0.6 to 1.5% by weight, and further, manganese (Mn) , Iron (Fe), copper (Cu), zinc (Zn) and strontium (Sr) may be included.

Liver water, which is an active ingredient of the cosmetic composition of the present invention, also has an excellent moisturizing effect, and as a result of confirming the results of experiments on the cell migration rate and the ability to produce collagen, as well as excellent anti-wrinkle or improvement effect, the substance derived from natural substance is an active ingredient. Therefore, the effect is remarkably excellent in being hypoallergenic and safe. Therefore, the cosmetic composition of the present invention can be applied to the skin to prevent wrinkles.

Therefore, the cosmetic composition of the present invention may be a cosmetic composition for preventing or improving wrinkles or a cosmetic composition for anti-wrinkle.

The cosmetic composition may contain 0.0001 to 50% by weight, preferably 0.001 to 30% by weight, based on the total number of the composition in the skin. That is, the content of the liver water should be at least a minimum value in order to achieve an anti-wrinkle effect, but can be freely controlled within the above ranges in consideration of whether the skin is irritated and the feeling of use is reduced due to excessive addition and its applicability to various formulations. At this time, the content of the active ingredient and the content of the additional component may be appropriately adjusted within the above range according to the content of the components contained in the formulation or cosmetic composition.

The cosmetic composition of the present invention may be provided for use in skin external ointments, basic cosmetics, makeup cosmetics, body cosmetics or shaving cosmetics, and the like, and examples of the basic cosmetics include essences, lotions, creams, gels, lotions, packs, massages, and the like. There may be creams, latexes, and the like, and examples of the makeup cosmetics may include foundation, lipstick, eyeshadow, eyeliner, mascara, eyebrow pencil, makeup base, and the like, and body cosmetics include soap, liquid cleaner, and bathing agent. , Sun screen cream, sun oil and the like, and shaving cosmetics include aftershave, shaving cream and the like.

The cosmetic composition of the present invention may further include a substance such as a functional substance having wrinkle improvement or moisturizing effect, an excipient for improving physical properties, a diluent or the like, in addition to containing the water as an active ingredient.

For example, the cosmetic composition may be additionally added to the perfume, pigments, fungicides, antioxidants, preservatives, moisturizers, thickeners, inorganic salts or synthetic polymer materials to improve physical properties. In addition to the blending components that may be added, it may be a fat or oil component, an emollient agent, a surfactant, an organic pigment, an inorganic pigment, an organic powder, an ultraviolet absorber, a pH adjuster, an alcohol, a blood circulation accelerator, a cooling agent, a limiting agent, or purified water.

In addition, the cosmetic composition may further comprise one or more selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The compounding component which may be added other than the said active ingredient is not limited to this, Any said component can be mix | blended within the range which does not impair the objective and effect of this invention.

In addition, the cosmetic composition may take the form of a solution, an emulsion, a viscous mixture, and the like, and any formulation commonly prepared in the art, for example, emulsion, cream, lotion, pack, foundation, lotion, beauty liquid, hair cosmetic, etc. It may be a formulation.

More specifically, the cosmetic composition of the present invention skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition Formulations of essences, packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

For example, when the formulation of the present invention is a paste, cream or gel, the carrier component is animal fiber, vegetable fiber, wax, paraffin, starch, trakant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide. This can be used.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as the carrier component, and especially when the formulation of the present invention is a spray, additionally chlorofluoro Propellants such as rohydrocarbon, propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent, or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, Fatty acid esters of 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

As described above, the cosmetic composition comprising the liver water of the present invention as an active ingredient is confirmed through the cell migration rate measurement and collagen production ability, particularly anti-aging effect, excellent anti-wrinkle effect, excellent moisturizing effect, and also corresponds to food Since it is made from the natural salt, which is made of natural salt, safety is also guaranteed, and furthermore, it is economically advantageous because it can be prepared from the natural salt produced in abundance on the west coast of Korea, especially Jeollanam-do, and is economically advantageous. As the effect will be very large.

Hereinafter, preferred embodiments of the present invention will be described. However, the following examples are only preferred embodiments of the present invention, and the present invention is not limited to the following examples.

Example 1 Preparation of Brine and Mineral Component Analysis

Natural salt and saline of the present invention was purchased with Taepyeong Salt Co., Ltd. located in Jeungdo, Sinan-gun, Jeollanam-do, and aged salt was purchased from Bioplus, a school company based in Mokpo National University, Muan-gun, Jeollanam-do. In addition, the brine of the present invention was prepared by centrifuging the purchased natural salts for 320 seconds at 6,000 rpm and room temperature (15 to 20 ℃), by removing the solids and collecting the liquid only.

On the other hand, mineral analysis of the brine is diluted 1g of the sample in 1L of tertiary distilled water from which ions have been removed, and then the dilution is filtered through a syringe filter having a 0.25 μm pore size. Analysis was performed by plane method using Absorption Spectrometer (AAS, Hitachi Z-2300, Japan) and other trace minerals were analyzed by helium mode using Inductively Coupled plasma / MS (ICP / MS, Agilent 7500, USA). The results are shown in Table 1 below.

[Table 1]

Li (ppm) K (ppm) Mg (ppm) Sr (ppm) Mn (ppm) Na (ppm) Ca (ppm) Fe (ppm) bittern 43.6 41500 201500 14.4 17.19 112100 110 1.052

Example  2: Wrinkle related functional evaluation

In order to evaluate the wrinkle-related functionality of the liver, first, cytotoxicity was measured to identify stability problems, and experiments on cell migration rate and collagen production ability were performed in relation to anti-wrinkle or wrinkle improvement effects.

Example  2-1; Cytotoxicity test

In order to measure the cytotoxicity of the hepatitis, sun salt, saline, and purified salt of Example 1, cell growth and inhibition rate were measured in skin cells, normal human dermal fibroblast and macrophage.

Specifically, MTT colometric assay was performed using CCD-1064Sk (normal human dermal fibroblast, ATCC CRL-2076, USA) to measure the cytotoxicity of hepatic and sun salt, saline, and purified salt in relation to fibroblasts. First, 200 μL / well of CCD-1064Sk was injected into a 96 well plate at a concentration of 1 × 10 ⁵ cells / mL, and incubated at 37 ° C. and 5% CO ₂ for 24 hours. And final concentrations of the purified salt were treated to 9 mg / mL, 18 mg / mL, 45 mg / mL, and 90 mg / mL, respectively. After incubating the cells treated with the sample for 24 hours, 20 μL of MTT reagent prepared at a concentration of 2 mg / mL was added thereto and cultured for 4 hours to form formazan. 200 μL of dimethylsulfoxide (DMSO, Sigma, USA) was dissolved in the formazan formed on the bottom of the 96 well plate, and the absorbance was measured at 540 nm using an ELISA reader. The measured values were analyzed by relative cell growth and inhibition rate when the absorbance of the control was 100%, and the results are shown in Table 2.

TABLE 2

In addition, MTT colometric assay was performed using Raw264.7 (Raw264.7 cell line, ATCC TIB-71, USA) to measure the cytotoxicity of hepatitis, sun salt, saline, and purified salt in relation to macrophages. First, dispensing Raw264.7 into a 96 well plate at a concentration of 5 × 10 ⁴ cells / mL at 200 μL / well and incubating at 37 ° C. and 5% CO ₂ for 24 hours. And final concentrations of the purified salt were treated to 9 mg / mL, 18 mg / mL, 45 mg / mL, and 90 mg / mL, respectively. After incubating the cells treated with the sample for 24 hours, 20 μL of MTT reagent prepared at a concentration of 2 mg / mL was added thereto and cultured for 4 hours to form formazan. 200 μL of dimethylsulfoxide (DMSO, Sigma, USA) was dissolved in the formazan formed on the bottom of the 96 well plate, and the absorbance was measured at 540 nm using an ELISA reader. The measured values were analyzed by relative cell growth and inhibition rate when the absorbance of the control was 100%, and the results are shown in Table 3.

[Table 3]

As shown in Table 2 and Table 3, the liver water showed cytotoxicity at a concentration of 9 mg / mL, and the cytotoxicity was not shown at a concentration of 4.5 mg / mL or less, and the cosmetic was based on the above experimental results. The proper amount of water used for the table composition was set at a concentration of 4.5 mg / mL or less.

Example  2-2; Cell migration rate test

In order to measure the cell migration rate of the hepatitis, sun salt, saline and purified salt of Example 1, a migration assay was performed on normal human dermal fibroblasts, skin cells.

Specifically, CCD-1064Sk (normal human dermal fibroblast, ATCC CRL-2076, USA) was added to 1 × 10 ⁵ cells / mL in 24 well plates for measuring cytotoxicity of hepatic and sun salt, saline, and purified salts in relation to fibroblasts. Dispense 500μL / well at a concentration of 500 μL / well, incubate at 37 ° C and 5% CO ₂ for 48 hours, and then wound on the bottom of a 24 well plate with a sterile tip when CCD-1064Sk grew to 90% or more of the well. scratch) and washed twice with PBS to remove fallen cells. After removing the cells, 500 μL / well of IMV (iscove's modified dulbecco's medium) containing no fetal bovine serum (FBS) was added thereto. In order to control the cell proliferation of the wound scratch part of the cells, the cells were treated with mitomycin c (Sigma, USA) for 1 hour, and the medium was replaced with new IMDM without FBS.

The final concentrations of the brine and sun salt, saline and purified salt were treated to be 1.125 mg / mL, 2.25 mg / mL and 4.5 mg / mL, respectively. After culturing the cells treated with the sample for 24 hours, the number of transferred cells was stained using giemsa stain, and observed using a microscope image, the results are shown in FIG.

As shown in FIG. 1, in the case of saline, cell movement was limited at a certain concentration, whereas in case of brine, cell movement was increased in proportion to the concentration, and the increase in the cell movement was also treated at the same concentration. As a result of the comparison, it was found to be superior to the sun salt and the purified salt.

Example  2-3; Collagen On generation capacity  Examination

In order to determine the effect on the collagen production capacity of the liver water, sun salt, saline and purified salt of Example 1, collagen production capacity was confirmed by measuring the extracellular free collagen content in skin cells fibroblasts (normal human dermal fibroblast) .

Specifically, Sirius red dye binding assay was performed using CCD-1064Sk (normal human dermal fibroblast, ATCC CRL-2076, USA) to measure the collagen production capacity of hepatic and sun salt, saline, and purified salt in relation to fibroblasts. Firstly, CCD-1064Sk was dispensed into 300 wells at a concentration of 1 × 10 ⁵ cells / mL in a 24 well plate and incubated at 37 ° C. and 5% CO ₂ for 24 hours. And purified salt was added to IMDM containing 10% FBS for each concentration within a range that does not exhibit cytotoxicity was dispensed by 300μL in each well.

The divided cells were incubated for 72 hours at 37 ° C. and 5% CO ₂ , and the medium exchanged during the culture was refrigerated at 4 ° C. to obtain the total collagen content released into the medium. After adding 6 mL of 50 μM sirius red solution (0.5M acetic acid) to 600 μL of the stored medium, the reaction was performed at room temperature for 20 min. After the reaction, the mixture was centrifuged at 5000 × g for 20 minutes, and 200 μL of 0.5 M NaOH was added to the precipitate to elute collagen for 5 minutes. The eluted collagen dye (collagen dye) was transferred to a 96well plate and the absorbance value was measured at 540 nm to obtain the total collagen content released extracellularly, and the results are shown in Table 4.

[Table 4]

As shown in Table 4 above, when treated with liver water, the amount of collagen synthesis was remarkably superior as compared with the treatment with sun salt, saline or purified salt, and especially when salt water was treated with a minimum concentration, salt samples with different amounts of collagen production were prepared. It was found to be remarkably superior to the case of treatment at the maximum concentration.

Example  3: evaluation of moisture retention

In order to measure the effect of liver water, sun salt and saline of Example 1 on skin moisturization, transdermal moisture evaporation rate (TE paper, g / hmn ² ) was measured using MPA-5.

Specifically, 25 volunteers in their 20s and 20 years of age were divided into 5 groups of 5 people each, untreated group, cream treated group, creamed group containing brine, creamed group containing brine and creamed salt containing sun salt. Percutaneous water evaporation rate was measured by dividing into groups.

The cream was purchased by using a cream base for manufacturing cosmetics (Thruingen Korea), and the sample was used to mix the sample in the cream base by adding 4.5 mg of each sample per 1 g of each cream base and stirring for 5 minutes.

In other words, after dividing each group into 2 hours before entering the laboratory, and adapted to the laboratory environment, 0.5g of each sample was applied to the skin 20 × 20 mm ² area, 0 minutes, 10 minutes, 30 minutes, At 60 and 120 minutes, transdermal moisture loss was measured using MPA-5, and the average value is shown in FIG. 2. The laboratory environment was 25.9 ° C. and relative humidity 54.4.

As shown in FIG. 2, when the sun salt or brine was treated with the cream, the result was found to be inferior to that of the non-treated group, but when the cream was treated alone when the liver and the cream were treated together. It was confirmed that the moisturizing effect is much superior to that, the liver was found to have an excellent effect in relation to skin moisturizing.

In summary, the liver water is excellent in terms of cell mobility, collagen production capacity, and skin moisturizing effect, and is excellent in anti-wrinkle effect, and is manufactured from salt, which is a natural substance and food, and thus, a cosmetic comprising the water of the present invention. It is expected that the composition may be applied in several directions with respect to wrinkle prevention or wrinkle improvement.

Manufacturing example

Preparation Example 1 Preparation of Skin Ointment

To the skin ointment was prepared in the composition of Table 5 (unit: wt%).

TABLE 5

Composition weight % bittern
Diethyl sebacate
Prepayment
Polyoxyethylene Oleyl Ether Phosphate
Sodium benzoate
vaseline 0.01
8
5
6
Suitable amount
to 100

Preparation Example 2: Preparation of Cosmetics (Cream)

To prepare a cream in the composition of Table 6 (unit: wt%).

TABLE 6

Composition weight % bittern
Stearic acid
Cetanol
Fiji-20 sorbitan monostearate
Sorbitan monostearate
Mineral oil
Trioctanoate
Triethanolamine
Carbomer
glycerin
Propylene glycol
antiseptic
incense
Purified water 0.01
1.0
2.0
1.0
1.0
10.0
5.0
0.5
0.2
5.0
3.0
Suitable amount
Suitable amount
to 100

Preparation Example 3 Preparation of Cosmetic (Flexible Cosmetic)

To a flexible cosmetics was prepared in the composition (unit: weight%) of Table 7 below.

TABLE 7

Composition weight% bittern
ethanol
Polyoxyethylene Cured Castor Oil
Paraoxybenzoic Acid Methyl
glycerin
1,3-butylene glycol
incense
Pigment
Purified water 0.02
10.0
1.0
2
5.0
6.0
Suitable amount
Suitable amount
to 100

Preparation Example 4 Preparation of Cosmetic (Flexible Cosmetic)

To the flexible composition was prepared in the composition of Table 8 (unit: wt%).

[Table 8]

Composition weight% bittern
Propylene glycol
glycerin
Sodium hyaluronate aqueous solution (1%)
ethanol
Polyoxyethylene Cured Castor Oil
Paraoxybenzoic Acid Methyl
Carbomer
Triethanolamine
incense
Purified water 0.2
10.0
10.0
5.0
5.0
1.0
0.1
0.3
0.4
Suitable amount
to 100

Preparation Example 5 Preparation of Cosmetic (Flexible Cosmetic)

To the flexible composition was prepared in the composition (unit: wt%) of Table 9 below.

TABLE 9

Composition weight% bittern
glycerin
Propylene glycol
Polyvinyl alcohol
ethanol
Polyoxyethylene Cured Castor Oil
Polyoxyethylene Oleethyl
Paraoxybenzoic Acid Methyl
incense
Pigment
Purified water 0.01
5.0
4.0
15.0
8.0
1.0
1.0
0.2
Suitable amount
Suitable amount
to 100

1 is a photograph observing the effect of the salt sample on the migration rate of fibroblasts according to an embodiment of the present invention, the liver water, sun salt, purified salt and saline of 1.125mg / ml, 2.25mg / ml and 4.5mg / ml, respectively It is the photograph which shows the result of processing by the density | concentration of.

Figure 2 is a graph confirming the effect of the salt sample according to an embodiment of the present invention on the moisturizing effect through the measurement of transdermal moisture evaporation rate.

Claims (4)

Centrifuging the natural salt for 320 seconds at 6,000 rpm and 15 to 20 ° C., removing solids and collecting only liquid Method of producing a liver having a wrinkle improvement effect comprising a. delete delete delete

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