KR101095856B1 - 효소면역학적 방법을 이용한 유류제품의 이력 추적 및 식별방법 - Google Patents
효소면역학적 방법을 이용한 유류제품의 이력 추적 및 식별방법 Download PDFInfo
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- KR101095856B1 KR101095856B1 KR1020090009283A KR20090009283A KR101095856B1 KR 101095856 B1 KR101095856 B1 KR 101095856B1 KR 1020090009283 A KR1020090009283 A KR 1020090009283A KR 20090009283 A KR20090009283 A KR 20090009283A KR 101095856 B1 KR101095856 B1 KR 101095856B1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B60—VEHICLES IN GENERAL
- B60B—VEHICLE WHEELS; CASTORS; AXLES FOR WHEELS OR CASTORS; INCREASING WHEEL ADHESION
- B60B31/00—Apparatus or tools for assembling or disassembling wheels
- B60B31/06—Apparatus or tools for assembling or disassembling wheels for removing or attaching cover discs, hub caps, or the like
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/26—Oils; Viscous liquids; Paints; Inks
- G01N33/28—Oils, i.e. hydrocarbon liquids
- G01N33/2835—Specific substances contained in the oils or fuels
- G01N33/2882—Markers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B60—VEHICLES IN GENERAL
- B60B—VEHICLE WHEELS; CASTORS; AXLES FOR WHEELS OR CASTORS; INCREASING WHEEL ADHESION
- B60B27/00—Hubs
- B60B27/0047—Hubs characterised by functional integration of other elements
- B60B27/0057—Hubs characterised by functional integration of other elements the element being a brake drum
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Mechanical Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Claims (8)
- 유류제품에 친유성기와 친수성기가 동시에 존재하는 식별제를 첨가하는 단계;상기 유류제품으로부터 계면활성제를 이용하여 식별제를 추출하는 단계; 및상기 추출된 식별제를 특이적인 항체를 이용하여 효소-면역학적 방법으로 검출하는 단계를 포함하는 유류제품의 이력 추적 및 식별 방법.
- 제 1항에 있어서, 상기 유류제품은 가솔린 또는 디젤유인 것을 특징으로 하는 유류제품의 이력 추적 및 식별 방법.
- 제 1항에 있어서, 상기 식별제는 2,4,5-트리클로로페녹시아세트산 (2,4,5-Trichlorophenoxyacetic acid) 또는 2,4-디클로로페녹시아세트산 (2,4-Dichlorophenoxyacetic acid)인 것을 특징으로 하는 유류제품의 이력 추적 및 식별 방법.
- 제 1항에 있어서, 상기 특이적인 항체는 2,4,5-트리클로로페녹시아세트산 또는 2,4-디클로로페녹시아세트산과 운반체 단백질(carrier protein)의 복합체에 의해 생성된 토끼 유래의 2,4,5-트리클로로페녹시아세트산 또는 2,4-디클로로페녹시아세트산 항체인 것을 특징으로 하는 유류제품의 이력 추적 및 식별 방법.
- 제 4항에 있어서, 상기 운반체 단백질은 키홀림펫헤모시아닌 (keyhole limpet hemocyanin ; KLH) 또는 소혈청알부민 (Bovine Serum Albumin; BSA)인 것을 특징으로 하는 유류제품의 이력 추적 및 식별 방법.
- 제 1항에 있어서, 효소-면역학적 방법은 별도로 준비된 항원물질과의 경쟁반응을 이용하여 저해율을 측정하는 것을 특징으로 하는 유류제품의 이력 추적 및 식별 방법.
- 2,4,5-트리클로로페녹시아세트산 (2,4,5-Tichlorophenoxyacetic acid) 또는 2,4-디클로로페녹시아세트산 (2,4-Dichlorophenoxyacetic acid)을 포함하는 친유성기(親油性基)와 친수성기(親水性基)가 동시에 존재하는 것을 특징으로 하는 유류 식별제.
- 2,4,5-트리클로로페녹시아세트산 (2,4,5-Tichlorophenoxyacetic acid) 또는 2,4-디클로로페녹시아세트산 (2,4-Dichlorophenoxyacetic acid)과 운반체 단백질(carrier protein)의 복합체를 이용하여 제조된 토끼 유래 2,4,5-트리클로로페녹시아세트산 (2,4,5-Tichlorophenoxyacetic acid) 또는 2,4-디클로로페녹시아세트산 (2,4-Dichlorophenoxyacetic acid) 항체를 포함하는 유류제품로부터의 식별제 검출 키트.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020090009283A KR101095856B1 (ko) | 2009-02-05 | 2009-02-05 | 효소면역학적 방법을 이용한 유류제품의 이력 추적 및 식별방법 |
PCT/KR2010/000676 WO2010090450A2 (en) | 2009-02-05 | 2010-02-04 | Tracing and detection method of oil product by enzyme-linked immunosorbent assay technology |
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US5691148A (en) | 1992-12-01 | 1997-11-25 | Ensys, Inc. | Petroleum immunoassay method, its components and a kit for use in performing the same |
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