KR101087721B1

KR101087721B1 - Luminometer

Info

Publication number: KR101087721B1
Application number: KR20100004487A
Authority: KR
Inventors: 김경남
Original assignee: (주)마이크로디지탈
Priority date: 2010-01-18
Filing date: 2010-01-18
Publication date: 2011-11-30
Also published as: KR20110084761A

Abstract

The present invention relates to a light emission measuring apparatus for measuring the light emission of a luminescent living body reacting with a light emitting expression material contained in a sample, the test table formed with a plurality of reaction chambers; A sample supply device for supplying a sample to the reaction chambers; A reagent supply device for supplying a reagent to the reaction chambers; A luminescence detection device for detecting luminescence emitted by the luminescent living body contained in the reagent in response to the sample; And a temperature control device installed at the test table and adjusting the temperature of the sample and the reagent contained in the reaction chambers, wherein the temperature control device includes a heat exchange pocket in which a heat medium circulation path is formed directly below the reaction chambers. ; A heat medium line for supplying a heat medium to the heat exchange pocket; And a heat medium circulator connected to the heat medium line to circulate the heat medium in the heat medium line, so that reliability and repeatability of the measured value can be greatly improved, immediately after dispensing, and after 15 minutes, 30 minutes After 60 minutes, etc., it is possible to have the world's highest level of uniform and precise scattering according to the time, and has the effect of minimizing the amount of reagents discarded by preventing the foaming of reagents.

Description

Luminescence measuring device {Luminometer}

The present invention relates to a luminescence measuring device, and more particularly, to a luminescence measuring device for measuring the emission of a luminescent living body reacting with the luminescent expression material contained in the sample.

A luminescence measuring device for measuring the emission of a luminescent living body reacting with the luminescent expression material contained in the sample has been developed and used. Such a luminescence measuring device is applied to and used in the fields of water quality testing, medical research, biological ecology research, or genetic research. In general, a conventional luminescence measuring device supplies samples and reagents to a plurality of reaction chambers, and detects luminescence emitted by the luminescent living body contained in the reagent reacting with the sample.

However, such a conventional luminescence measuring device has a problem in that reliability of the measured value is greatly reduced due to the characteristics of a luminescent living body which is very sensitive to temperature because it is very difficult to control the real-time temperature of samples and reagents contained in a plurality of reaction chambers.

That is, for example, if the same sample and the same reagent are put into a total of 96 reaction chambers in 12 rows of 8 rows, the same result should be theoretically obtained, but in practice, the temperature deviation between the first reaction chamber and the last reaction chamber is measured. Due to the error about the measurement is too severe, there was a problem that the reliability and repeatability of the measurement is greatly reduced.

SUMMARY OF THE INVENTION An object of the present invention for solving the above problems is to provide a luminescence measurement device that can greatly improve the reliability and repeatability of the measured value by using a heat exchange pocket having a reaction chamber and a heat medium circulation path corresponding to the test table. .

In addition, another object of the present invention is to arrange the heat medium circulation path of the heat exchange pocket in a zigzag shape corresponding to the reaction chamber so that after the test dispensing, after 15 minutes, 30 minutes, 60 minutes, etc., the world's highest uniformity according to time. An object of the present invention is to provide a light emission measuring apparatus capable of having a scatter diagram.

In addition, another object of the present invention is to provide a luminescence measurement apparatus that can minimize the amount of reagents discarded by preventing bubble formation of reagents.

A luminescence measuring apparatus of the present invention for achieving the above object, the luminescence measuring device for measuring the emission of a luminescent living body reacting with the luminescent expression material contained in the sample, the test bench formed with a plurality of reaction chambers; A sample supply device for supplying a sample to the reaction chambers; A reagent supply device for supplying a reagent to the reaction chambers; A luminescence detection device for detecting luminescence emitted by the luminescent living body contained in the reagent in response to the sample; And a temperature control device installed at the test table and adjusting the temperature of the sample and the reagent contained in the reaction chambers, wherein the temperature control device includes a heat exchange pocket in which a heat medium circulation path is formed directly below the reaction chambers. ; A heat medium line for supplying a heat medium to the heat exchange pocket; And a heat medium circulator connected to the heat medium line to circulate the heat medium inside the heat medium line.

In addition, according to the present invention, it is preferable that the heat medium circulation path of the heat exchange pocket is formed in a zigzag shape so as to pass directly below the plurality of reaction chambers.

In addition, the light emission measuring apparatus of the present invention, a transport device for transferring the inspection table or the light emission detection device relative to each other; And a controller configured to apply a heat medium circulation line following control signal to the transfer device such that the light emitting detection device is moved along the heat medium circulation line.

In addition, according to the present invention, the conveying apparatus, the inspection table conveying device for sequentially conveying the inspection table in the Y-axis direction; And a nozzle head transfer device configured to sequentially transfer a nozzle head provided with a sample supply nozzle of the sample supply device, a reagent supply nozzle of the reagent supply device, and the light emitting detection device in the X-axis direction.

In addition, according to the present invention, the reagent supply device, a reagent injection nozzle for introducing a reagent into the reaction chamber; A container holder containing a reagent container in which a reagent is stored; A reagent line connecting the reagent container accommodated in the container receiving container with the reagent injection nozzle; A reagent pump installed in the reagent line and transferring the reagent of the reagent container to the reagent injection nozzle; And a controller for applying an intermittent control signal having a predetermined period to the reagent pump so that the reagent is intermittently introduced into the reagent injection nozzle to prevent bubble generation of the reagent.

As described above, according to the light emission measuring apparatus of the present invention, the reliability and repeatability of the measured value can be greatly improved, and the world's highest uniformity according to the time immediately after the test dispensing, after 15 minutes, after 30 minutes, after 60 minutes, etc. It is possible to have a precise scatter plot, and to have the effect of minimizing the amount of reagents discarded by preventing the foaming of reagents.

1 is a front perspective view schematically showing a light emission measuring apparatus according to an embodiment of the present invention.
2 is a perspective view of FIG. 1.
3 is an enlarged perspective view illustrating an enlarged view of the nozzle head of FIG. 2.
4 is a plan view of FIG. 1.
5 is an enlarged perspective view illustrating the heat exchange pocket of FIG. 4.
6 is a plan view illustrating an example of a heat medium circulation path of the heat exchange pocket of FIG. 4.
7 is a plan view illustrating another example of the heat medium circulation path of the heat exchange pocket of FIG. 4.
8 is a photograph showing the inspection table transfer apparatus of FIG.
9 is a photograph showing an example of the heat medium circulation path of the heat exchange pocket of FIG. 6.
FIG. 10 is a table showing data values, percentage values, and dispersion coefficients (Coefficient of Variance (CV)) measured immediately after dispensing by dividing the sample and the reagent into the reaction chambers of FIG. 5.
FIG. 11 is a table illustrating data values, a percentage value, and a dispersion coefficient (Coefficient of Variance (CV)) measured after 15 minutes of injecting a sample and a reagent by dividing the reaction chambers of FIG. 5.
FIG. 12 is a table showing data values, percentage values, and dispersion coefficients (Coefficient of Variance (CV)) measured after 30 minutes of dividing and injecting a sample and a reagent into the reaction chambers of FIG. 5.
FIG. 13 is a table illustrating data values, a percentage value, and a dispersion coefficient (Coefficient of Variance (CV)) measured after 60 minutes of dividing and injecting a sample and a reagent into the reaction chambers of FIG. 5.

Hereinafter, a light emission measuring apparatus according to various exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

1 is a front perspective view schematically showing a light emission measuring apparatus according to a preferred embodiment of the present invention, FIG. 2 is a perspective view of FIG. 1, FIG. 3 is an enlarged perspective view showing an enlarged nozzle head of FIG. 2, and FIG. 4. Is a plan view of FIG.

First, as shown in FIGS. 1 to 4, a light emission measuring apparatus according to a preferred embodiment of the present invention is a light emission measuring apparatus for measuring light emission of a luminescent living body reacting with a light emitting expression material included in a sample. Case 1, test bench 10, sample supply device 20, reagent supply device 30, light emission detection device 40, temperature control device 50, transfer device 60 and The control unit 70 may be included.

Here, the case 1, as shown in Figures 1 and 2, the sample container 23 and the reagent container 32 may be formed to open and close the door (2) to flow in and out.

5 is an enlarged perspective view illustrating the heat exchange pocket of FIG. 4.

As shown in FIG. 5, the test table 10 includes a plurality of reaction chambers 11 formed therein, and the test table 10 includes a lid having inlets 12a corresponding to the reaction chambers 11, respectively. 12 can be installed.

On the other hand, as shown in Figure 1, the sample supply device 20, to supply a sample to the reaction chamber 11, the sample input nozzle 21 for feeding the sample into the reaction chamber (11) And a container container 22 for receiving the sample container 23 in which the sample is stored, and a sample line 24 for connecting the sample container 23 and the sample injection nozzle 21 accommodated in the container container 22 to each other. And a control signal applied to the sample pump 25 and the sample pump 25 which are installed in the sample line 24 and transfer the sample of the sample container 23 to the sample injection nozzle 21. The control unit 70 may be included.

Therefore, when explaining the operation relationship of the sample supply device 20 of the luminescence measuring device of the present invention, when the user loads the sample container 23 in the container receiving chamber 22, by the sample pump 25 A sample in the sample container 23 may be automatically supplied to the reaction chamber 11 through the sample injection nozzle 21 along the sample line 24 and through the inlet 12a of the cover 12. It is.

Therefore, the user can divide (inject) the samples of the sample container 23 into the plurality of reaction chambers 11 without relying on manual labor.

On the other hand, as shown in Figure 1, the reagent supply device 30, to supply the reagents to the reaction chamber 11, the reagent injection nozzle 31 for introducing the reagent into the reaction chamber (11) And, Receptacle container 22 for receiving the reagent container in which the reagent is stored, Reagent line 32 and the reagent line 33 connected to the reagent container 32 accommodated in the container receiving container 22, And The reagent pump 34 and the reagent which are installed in the reagent line 33 and transfer the reagent of the reagent container 32 to the reagent injection nozzle 31 are intermittently introduced into the reagent injection nozzle 31. The controller 70 may be configured to apply an intermittent control signal having a predetermined period to the reagent pump 34 so as to prevent bubble generation of the reagent.

Therefore, when explaining the operation relationship of the reagent supply device 30 of the luminescence measuring device of the present invention, when the user loads the reagent container 32 in the container receiving chamber 22, by the reagent pump 34 Reagents inside the reagent vessel 32 may be automatically supplied to the reaction chamber 11 along the reagent line 33 through the reagent injection nozzle 31 and past the inlet 12a of the lid 12. It is.

Here, for example, the above-described reagents may be used as reagents including luminescent organisms, for example, luminescent bacteria, enzymes, luminescent E. coli, luminescent strains, luminescent microorganisms, gene combinations, and the like. The sample may be a luminescent material capable of inducing luminescence by reacting with the reagent and may be a liquid material. The luminescent material is, for example, sewage, wastewater, wastewater treatment plant influent, wastewater treatment plant discharge, underground water, river water, drinking water, liquid substance extracted from sediments or soil, liquid substance dissolved in a liquid after adsorbing contaminants of gas. And the like, or may include any material capable of emitting luminescence used in the fields of medical research, bioeconomic research, or genetic research. However, such a sample is exemplary, and the present invention is not limited thereto.

In particular, the light emission measuring apparatus of the present invention can minimize the amount of reagents discarded by preventing bubble generation of the reagents by using the controller 70 for applying an intermittent control signal having a predetermined period to the reagent pump 34. It is.

That is, reagents containing luminescent living organisms are extremely expensive, and when the reagent pump 34 continuously transfers the reagents and bubbles are generated due to low pressure in the reagents, the reagents may not be smoothly transferred, It is possible to prevent as much of the reagent waste as possible to remove.

Here, the period of the intermittent control signal is preferably optimized in consideration of the transfer amount and the transfer speed of the reagent and the pumping capacity of the reagent pump 34.

The luminescence detection device 40 detects luminescence emitted by the luminescent living body contained in the reagent in response to the sample, and includes various types of optical signal detecting devices such as an optical sensor for sensing the luminescence of the luminescent living body. It can be applied.

In addition, as illustrated in FIG. 4, the transfer device 60 may be configured to transfer the inspection table 10 or the light emitting detection device 40 relatively to each other, and various types of actuators and motion converters may be applied. Preferably, it can be comprised largely including the test stand feeder 61 and the nozzle head feeder 62. FIG.

The inspection table transfer device 61 is a Y-axis screw rod 612 and the Y-axis penetrating the movable table 611 in which the inspection table 10 is installed to sequentially convey the inspection table 10 in the Y-axis direction. And a first motor 613 for rotating the shaft screw rod 612.

In addition, the nozzle head transfer device 62 may include a sample injection nozzle 21 of the sample supply device 20, a reagent injection nozzle 31 of the reagent supply device 30, and the light emitting detection device 40. A second motor 623 that rotates the X-axis screw rod 622 and the X-axis screw rod 622 passing through the nozzle head 621 so as to sequentially transfer the nozzle head 621 to be installed in the X-axis direction. It can be made to include.

Therefore, when describing the operation relationship of the transfer device 60 of the present invention, first, when the first motor 613 is rotated by receiving the Y-axis movement control signal of the control unit 70 (Y-axis screw rod ( When the movable table 611 moves the test bench 10 in the Y-axis direction while the 612 rotates, the control unit 70 supplies a supply control signal to the sample supply device 20 and the reagent supply device 30. It is possible to supply samples and reagents of the reaction chambers 11 of the first row among the plurality of reaction chambers 11, and when the supply of the reaction chambers 11 of the first row is completed, the control unit 70 When the second motor 623 is momentarily rotated by the X-axis movement control signal, the nozzle head 621 is moved in the X-axis direction of the test table 10 while the X-axis screw rod 622 is momentarily rotated. Samples and reagents of the reaction chambers 11 in the second row can be supplied.

On the other hand, after the sample and reagents are supplied to all the reaction chambers 11 or at least one or more reaction chambers 11, the above light is emitted in the same manner while the movable table 611 and the nozzle head 621 are moved relatively. The light emission can be detected while the detection device 40 is sequentially moved.

6 is a plan view illustrating an example of the heat medium circulation path of the heat exchange pocket of FIG. 4, FIG. 7 is a plan view illustrating another example of the heat medium circulation path of the heat exchange pocket of FIG. 4, and FIG. 9 is a photograph showing an example of the heat medium circulation path of the heat exchange pocket of FIG. 6.

On the other hand, the temperature control device 50 is to be installed on the test table 10, to control the temperature of the sample and the reagent contained in the reaction chamber (11).

The temperature control device 50 includes a heat exchange pocket 52 in which a heat medium circulation path 51 is formed directly below the reaction chambers 11, and a heat medium line for supplying a heat medium to the heat exchange pocket 52. 53) and the heat medium line 53 connected to the heat medium line 53 to circulate the heat medium inside the heat medium line 53.

6 and 9, the heat medium circulation path 51 of the heat exchange pocket 52 is formed in a zigzag shape in a first direction so as to pass directly below and below the plurality of reaction chambers 11. Alternatively, as shown in FIG. 7, it is possible to be formed in a zigzag shape in the second direction.

In addition, the controller 70 preferably applies a heating medium circulation path following control signal to the transfer device 60 so that the light emitting detection device 40 moves along the heating medium circulation path 51.

Therefore, the transfer device 60 transfers the light emitting detector 40 along the heat medium circulation path 51 to emit light of the reaction chambers 11 by the heat medium circulation path following control signal of the control unit 70. Since it can be detected, the temperature variation between each of the adjacent reaction chambers 11 is minimized, and since the light emitting device 40 can be detected by zigzag movement, light emission can be detected, thereby reducing light emission time by reducing copper wires. It can be.

FIG. 10 is a table showing data values, percentage values, and scatter plots (Coefficient of Variance; C.V.) measured immediately after dispensing by dividing the sample and the reagent into the reaction chambers of FIG. 5.

FIG. 11 is a table showing data values, a percentage value, and a scatter plot (Coefficient of Variance; C.V.) measured after 15 minutes of dividing and injecting a sample and a reagent into the reaction chambers of FIG. 5.

FIG. 12 is a table showing data values, a percentage value, and a scatter plot (Coefficient of Variance; C.V.) measured after 30 minutes of dividing and injecting a sample and a reagent into the reaction chambers of FIG. 5.

FIG. 13 is a table showing data values, a percentage value, and a scatter plot (Coefficient of Variance; C.V.) measured after 60 minutes of dividing and injecting a sample and a reagent into the reaction chambers of FIG. 5.

As shown in Figs. 10 to 13, immediately after the reagent dispensing, after 15 minutes, after 30 minutes, after 60 minutes, 12 columns of 8 rows, the same reagents as the same sample were added to a total of 96 reaction chambers, Looking at the measured value, the measured value is between 95% and 105% of the reference value. This means that changes in the amount of light emission that may occur during the inevitable time taken while measuring samples in 96 reaction chambers are minimized. That is, by constantly adjusting the temperature of the sample, it is possible to minimize the experimental error of the measured value, thereby providing a high reliability of the light emission measuring apparatus. In addition, even when the amount of luminescence was measured after a predetermined time after the sample and reagent dispensing, for example, after 15 minutes, after 30 minutes, and after 60 minutes, the measured value was 95% to 105% compared to the reference value. Therefore, even after a predetermined time after the sample and reagent dispensing, it is possible to provide a measurement value minimized error can provide a high reliability and ease of use of the luminescence measuring device.

In addition, the scatter plot of the measured values showed a minimum of 0.54 to a maximum of 2.11, and another sensitivity to temperature change was also 1.47 after 60 minutes after the dispensing, showing 1.47, and achieving 25% change in 1 hour. Is the world's highest level of dispersion with an improved dispersion of more than 30 percent.

However, the experimental conditions are based on the number 1 well of the Vibrio ficheries, the reagent used at 100 μl / well, the temperature control of the reagents and the test table is 15 degrees, the integration time is 0.5 sec, the delay time is 0, and the percentage is up to AH. And based on well number A of rows 1-12.

The present invention is not limited to the above-described embodiments, and of course, modifications may be made by those skilled in the art without departing from the spirit of the present invention. Therefore, the scope of the claims in the present invention will not be defined within the scope of the detailed description, but will be defined by the following claims and their technical spirit.

1: case 2: open the door
10: test bench 11: reaction chamber
12: cover 12a: slot
20: sample supply device 21: sample injection nozzle
22: container container 23: sample container
24: sample line 25: sample pump
30: reagent supply device 31: reagent injection nozzle
33: reagent line 34: reagent pump
40: light emitting detection device 50: temperature control device
51: heat medium circulation path 52: heat exchange pocket
53: heat medium line 54: heat medium cycle
60: feeder 61: inspection table feeder
611: movable table 612: Y-axis screw rod
613: first motor 62: nozzle head feeder
621: nozzle head 622: X-axis screw rod
623: second motor 70: control unit

Claims

In the luminescence measurement device for measuring the light emission of the luminescent living body reacts with the luminescent expression material contained in the sample,
A test table on which a plurality of reaction chambers are formed;
A sample supply device for supplying a sample to the reaction chambers;
A reagent supply device for supplying a reagent to the reaction chambers;
A luminescence detection device for detecting luminescence emitted by the luminescent living body contained in the reagent in response to the sample; And
It is installed on the test table, the temperature control device for adjusting the temperature of the sample and the reagent contained in the reaction chamber; includes;
The temperature control device,
A heat exchange pocket in which a heat medium circulation path is formed directly below the reaction chambers;
A heat medium line for supplying a heat medium to the heat exchange pocket; And
A heat medium circulator connected to the heat medium line to circulate the heat medium in the heat medium line;
Luminescence measuring apparatus comprising a.

The method of claim 1,
The heat medium circulation path of the heat exchange pocket, the luminescence measurement device characterized in that it is formed in a zigzag shape to pass directly below the plurality of the reaction chamber.

3. The method according to claim 1 or 2,
A transfer device for transferring the inspection table or the light emission detection device to each other; And
A control unit for applying a medium circulation line following control signal to the transfer device to move the light emitting detection device along the heat medium circulation line;
The luminescence measuring device further comprises.

The method of claim 3, wherein
The transfer device,
An inspection table conveying apparatus for sequentially conveying the inspection table in the Y-axis direction; And
A nozzle head transfer device configured to sequentially transfer a nozzle head provided with a sample supply nozzle of the sample supply device and a reagent supply nozzle of the reagent supply device, and the light emitting detection device in an X-axis direction;
Luminescence measuring apparatus comprising a.

3. The method according to claim 1 or 2,
The reagent supply device,
A reagent injection nozzle for introducing a reagent into the reaction chamber;
A container holder containing a reagent container in which a reagent is stored;
A reagent line connecting the reagent container accommodated in the container receiving container with the reagent injection nozzle;
A reagent pump installed in the reagent line and transferring the reagent of the reagent container to the reagent injection nozzle; And
A control unit for applying an intermittent control signal having a predetermined period to the reagent pump so that the reagent is intermittently introduced into the reagent injection nozzle to prevent bubble generation of the reagent;
Luminescence measuring apparatus comprising a.