KR101957710B1 - A portable chemiluminescence measuring device for biomaker screening - Google Patents

Abstract

The present invention relates to a portable chemiluminescence measuring device for biomarker screening. In particular, the portable chemiluminescence measuring device for biomarker screening can maximize accuracy and reliability of measurements, regardless of a place where the measurement takes place, by effectively mixing a urine sample and a reagent using a vibrating means and by keeping a temperature of the reagent constant using a constant temperature maintaining means. To this end, the portable chemiluminescence measuring device comprises: a case forming an outer appearance and including an entrance formed on one side thereof; a dark room including a rotating body, the rotating body including a body, a driving shaft installed in a center of the body, and a holder, wherein the body is installed on one side inside the case and includes an inlet corresponding to the entrance, and the holder is axially coupled to the driving shaft and reversely rotated by the driving shaft and permits a sample tube holder to be supported in a lower part on one side thereof; a reagent injection unit installed on one side of the dark room to inject the reagent to a sample tube in the sample tube holder; and a sensor installed in an air-tight manner with the inside of the body of the dark room to sense an amount of chemiluminescence chemically emitted from the sample tube holder, wherein a vibrating means for applying vibrations to the sample tube holder is installed in the holder, and a constant temperature maintaining means for keeping a temperature of the reagent constant at all times is installed in the reagent injection unit.

Description

[0001] The present invention relates to a portable chemiluminescence measuring device for biomarker screening,

More particularly, the present invention relates to a chemiluminescence measurement device for examining a portable biomarker (blood, urine, etc.) for which the accuracy of chemiluminescence measurement is improved through vibration means and constant temperature maintenance means ≪ / RTI >

Sodium is a necessary component of our body, but when overdosed is a risk factor for many diseases, the World Health Organization (WHO) recommends that the daily intake of sodium for adults should be less than 2000 mg (based on salt) have.

Excess intake of sodium causes hypertension, stroke, coronary artery disease, cardiovascular disease, and increases the risk of kidney disease, stomach cancer, and osteoporosis.

Hypertension is an important cause of almost all heart diseases. It is recommended by the WHO and the Korean Heart Association and the Korean Society of Hypertension to limit the daily intake of salt for prevention and control of hypertension.

Sodium is mostly absorbed into the small intestine along with glucose and chlorine ions, and excess sodium is released mainly by urine and sweat.

That is, when sodium is high, potassium is excreted outside the body, and conversely, when potassium is high, sodium is excreted outside the body.

This can be an indication of the presence of sodium or potassium in the body depending on the sodium and potassium emission ratio.

The sodium / potassium (Na / K) ratio for any urine can be used to determine the sodium content in the body of patients who need a low-salt diet such as epilepsy, congestive heart failure, hypertension, diabetes, kidney disease and cardiovascular disease It can be used as an indicator.

However, in order to measure sodium / potassium (Na / K) ratio contained in the urine as described above, measurement is performed through admission to a large-sized hospital equipped with measurement equipment. Therefore, .

That is, it is difficult to easily grasp the sodium / potassium (Na / K) ratio in the body without requiring the hospital.

In order to solve such a problem, a portable measuring instrument has recently been developed as disclosed in Korean National Publication No. 10-2015-0102881 and Korean Registered No. 10-2016-1736331.

When a urine sample is injected through such a portable measuring device, a sample is injected from the reagent injecting part into the urine sample, and chemiluminescence is performed through a chemical reaction between the urine sample and the reagent.

Thereafter, the detection unit detects the chemiluminescence, and the chemiluminescence amount according to the content of sodium and potassium in the urine can be measured.

However, the above-described conventional portable measuring instrument has the following problems.

First, since the mixing of the urine sample and the reagent is performed only by the simple injection of the reagent, the mixing efficiency between the urine sample and the reagent is not maximized.

In the measurement of the amount of light emitted through the chemical reaction between the reagent and the urine sample, the mixing ratio of the reagent and the urine sample is of significance. As in the prior art, when the reagent is simply injected into the urine sample, There is a problem that it is difficult to increase the accuracy of the result.

Second, the portable measuring instrument has a characteristic that measurement can be performed regardless of time and place. Conventional measuring instrument has a problem that the external environment, especially temperature, is constrained.

That is, when the external temperature is high, the temperature of the reagent also rises, making it difficult to optimize the chemical reaction after mixing with the urine sample.

Korea Publication No. 10-2015-0102881 Korean Registration No. 10-2016-1736331

SUMMARY OF THE INVENTION It is an object of the present invention to provide a chemiluminescence quantitative measurement device for a portable biomarker examination, which is equipped with a vibrating means for applying a vibration to a sample tube so as to maximize a mixing ratio of a urine sample and a reagent, .

Another object of the present invention is to provide a device for measuring the amount of chemiluminescence for a portable bio-indicator which can optimize the temperature of the reagent injecting unit so that a reagent having an optimal temperature can be mixed with urine samples irrespective of the external environment.

In order to achieve the above-described object, the present invention provides a portable terminal comprising: a case having an outer appearance and having a door formed at one side thereof; a body provided at one side of the case and having a mouth corresponding to the doorway; A rotary shaft rotatably and rotatively driven by the driving shaft, and a rotatable body provided with a mounting part for receiving a sample tube holder at one side of the rotary shaft; And a sensor for detecting the amount of chemiluminescence chemiluminescence emitted from the sample tube holder, wherein the mounting part is provided with a reagent injecting part for injecting a sample into the sample tube holder, The reagent injecting unit is provided with a constant temperature maintaining means for keeping the reagent temperature constant at all times And a reagent for measuring the amount of chemiluminescence emitted from the biomarker.

In this case, it is preferable that the incubation maintaining means includes a temperature sensor for sensing the temperature of the reagent injecting portion, and a semiconductor cooler for maintaining the constant temperature of the reagent in the reagent injecting portion through temperature sensing by the temperature sensor.

Further, the sample tube holder is formed to have a curvature corresponding to the curvature of the rotating body, a coupling groove is formed on the bottom surface of the sample tube holder, and the mounting portion is protruded from the bottom of the rotating body so as to be fitted into the coupling groove And the vibrating means is provided as a vibration motor provided in the mounting portion.

It is preferable that the inner circumferential surface of the dark room body is formed with concavities and convexities along the inner circumferential surface, and the upper surface of the rotating body is formed with concavities and convexities in a concentric circular direction.

The apparatus for measuring the amount of chemiluminescence for a portable bio-indicator according to the present invention has the following effects.

First, a vibrating means is provided below a sample tube holder in which a sample tube in which a urine sample and a reagent are mixed is placed, and vibrations are applied to the sample tube through the vibrating means, thereby mixing a urine sample and a reagent Can be effectively achieved.

Thereby, the chemical reaction in the sample tube can be optimized, and the accuracy with respect to the chemiluminescence amount measurement value can be improved.

Secondly, since the constant temperature maintaining means is provided in the cage of the reagent injecting portion provided with a plurality of reagents to optimize the temperature of the reagent, the temperature of the reagent can be kept constant regardless of the external environment.

Accordingly, as the optimized reagent is always mixed with the urine sample, the efficiency of the chemical reaction can be maximized, and the accuracy and reliability of the measured value can be increased.

1 is a perspective view showing a device for measuring a chemiluminescence amount for examining a biomarker according to a preferred embodiment of the present invention.
FIG. 2 is an internal perspective view illustrating an internal configuration of a chemiluminescence amount measurement apparatus for examining a biomarker according to a preferred embodiment of the present invention. FIG.
FIG. 3 is an exploded perspective view showing a dark room constituting an apparatus for measuring a chemiluminescence amount for examining a biomarker according to a preferred embodiment of the present invention. FIG.
4 is a perspective view showing a dark room constituting an apparatus for measuring a chemiluminescence amount for biomarker marker examination according to a preferred embodiment of the present invention
FIG. 5 is a block diagram showing a main part of a chemiluminescence amount measurement apparatus for examining a biomarker according to a preferred embodiment of the present invention. FIG.

It is to be understood that the words or words used in the present specification and claims are not to be construed in a conventional or dictionary sense and that the inventor can properly define the concept of a term in order to describe its invention in the best possible way And should be construed in light of the meanings and concepts consistent with the technical idea of the present invention.

Hereinafter, the apparatus for measuring a chemiluminescence amount for a portable biomarker examination according to a preferred embodiment of the present invention (hereinafter referred to as a "chemiluminescence amount measuring apparatus") will be described with reference to FIGS. 1 to 5 attached hereto.

The chemiluminescence measuring device has a technical feature that it can measure the amount of light emitted through a chemical reaction with a sample of a body fluid to examine a biomarker, and provides a portable type for easy measurement regardless of time and place.

Precisely, it is possible to examine the disease state through measurement of sodium (Na) and potassium (K) ratio contained in the urine. It is necessary to increase the mixing efficiency of the urine sample and the reagent, Accuracy and reliability.

1 and 2, the apparatus includes a case 100, a dark room 200, a reagent injecting unit 300, and a sensing sensor 400.

The case 1000 constitutes an appearance of the chemical-mass-emission measuring apparatus, and an entrance 110 is formed at one side.

The entrance 110 is a passage through which the urine sample tube enters and exits the inside of the case 100. An opening and closing cover 111 for opening and closing the entrance 110 is hinged to the entrance 110.

Next, the dark room 200 is a space in which the amount of chemiluminescence emitted from the urine sample tube is measured, and is installed on one side of the inside of the case 100.

The dark room 200 provides a space for measuring the amount of chemiluminescence reacted through the mixing of the urine sample and the reagent, but it is required to ensure confidentiality so that the external light source can not be introduced.

Since the quantity of light generated through the chemical reaction is extremely stray light and it is difficult to increase the accuracy of measurement when the airtightness with the outside can not be maintained, the dark room structure can be constructed so that the external light source can be shut off.

2 and 3, the dark room 200 includes a body 210, a driving shaft 220, and a rotating body 230.

The body 210 constitutes an outer appearance of the dark room 200, and a space for emitting light from the sample tube is formed therein.

An inlet 211 corresponding to an entrance 110 formed in the case 100 is formed at one side of the body 210 and a sensor 400 to be described later is installed at the other side of the body 210, So that the polarized stray light is detected.

The body 210 is preferably cylindrical, and the upper portion is preferably open for replacement and maintenance.

At this time, a lid 212 is provided to shield the opened portion of the body 210.

Meanwhile, in order to increase the efficiency of detecting the amount of light emitted from the inside of the dark room 200, the dark room 200 may not reach the space of the body 210 even if an external light source is introduced, To be scattered.

To this end, concave and convex portions 210a are formed along the inner circumferential surface of the inner circumferential surface of the body 210, and concave and convex portions 210a continuous in the concentric circular direction are formed on the inner surface of the lid 2120.

The driving shaft 220 is configured to rotate the rotating body 230 in the space of the body 210 and is installed perpendicularly to the space bottom of the body 210.

The drive shaft 220 includes a motor (not shown), and is installed to be rotated in the forward and reverse directions by the power of the motor.

The rotating body 230 receives the sample tube through the entrance 110 of the case 100 and the inlet of one side of the body 210 and corresponds to the detection sensor 400 installed on the other side of the body 210 And is axially coupled to the drive shaft 220.

The sample tube holder 240 is detached from the rotating body 230.

3, the sample tube holder 240 provides a space for accommodating a plurality of sample tubes accommodating the urine sample, and the inlet 110 of the case 100 and the inlet 211 of the body 210 As shown in FIG.

The sample tube holder 240 is formed in an arc shape having a curvature corresponding to the curvature of the body 210 and is detachably attached to a mounting portion described later.

In the sample tube holder 240, a detection hole 241 is formed so that the detection sensor 400 can detect the amount of light emitted from the sample tube.

Further, a coupling groove 242 is formed on the bottom surface of the sample tube holder 240 so as to be detachable to the mounting portion.

Preferably, the coupling groove 242 is integrally formed along the curvature of the sample tube holder 240.

The rotating body 230 is formed in a cylindrical shape corresponding to the body 210 and a concave and a convex portion 210a for scattering the light transmitted into the body 210 is formed on the upper surface of the rotating body 230.

The concave and convex portions 210a correspond to the concave and convex portions 210a formed in the lid 212 and the concave and convex portions 210a are formed to be offset from each other so that the coupling between the rotor 230 and the lid 212 can be performed.

A mounting portion 231 is formed on one side of the rotating body 230 so that the sample tube holder 240 can be mounted.

One side of the rotating body 230 is cut in a shape corresponding to the height of the sample tube holder 240, and the mounting portion 231 is installed in the cut bottom.

The mounting portion 231 corresponds to the coupling groove 242 formed on the bottom surface of the sample tube holder 240 and is formed in the shape of a protrusion formed from the bottom of the rotating body 230 so as to be fitted into the coupling groove 242 .

With this configuration, the sample tube holder 240 in which the sample tube is accommodated can be detached from the mounting portion 231.

On the other hand, the mounting portion 231 is provided with a vibration means 231a.

The vibrating means 231a acts to apply a vibration to the sample tube of the sample tube holder 240 coupled to the mounting portion 231 so that the urine sample contained in the sample tube and the reagent can be evenly mixed.

That is, by allowing the urine and the reagent in the sample tube to be uniformly mixed, the amount of light emission can be maximized, and the detection efficiency through the detection sensor 400 can be increased.

The vibrating means 231a is preferably provided as a vibration motor, though not limited thereto.

The sample tube is caused to flow by the vibration generated through the vibration motor 231a, thereby maximizing the mixing ratio of the urine and the reagent.

At this time, the operation of the vibration motor 231a is performed through the control unit 500, and the vibration time can be adjusted through the timer 510 (refer to FIG. 5).

The vibration motor 231a vibrates the sample tube through the mounting portion 231 by applying vibration to the mounting portion 231 and exposes the vibration motor 231a to the upper portion of the mounting portion 231, (Not shown).

Next, the reagent injector 300 injects a reagent for chemical reaction with the urine sample into the sample tube, and is installed inside the case 100 as shown in FIG.

A plurality of reagents for injecting the reagent are provided and are partitioned and provided inside the cage 310.

Examples of reagents include an aptamer for capturing sodium, TPA for catalyst, TMPG, and the like, and as the reagent is mixed with the urine sample, chemiluminescence occurs from the urine sample through chemical reaction.

The above-mentioned reagents are reagents of known art, and need not be limited to specific ones.

On the other hand, the reagent injector 300 is further provided with a constant temperature holding means 320.

The constant temperature maintaining unit 320 serves to constantly maintain the reagent temperature of the reagent injecting unit 300 and is mounted on the cage 310 of the reagent injecting unit 300 as shown in FIG.

That is, the incubation means 320 maintains the reagent temperature at a constant level, thereby increasing the accuracy of the chemiluminescence measurement value at any place regardless of the external environment.

As shown in FIG. 5, the constant temperature maintenance unit 320 includes a temperature sensor 321 and a semiconductor cooler 322.

The temperature sensor 321 senses the temperature of the reagent injector 300. If the temperature sensor 321 senses that the temperature of the reagent injector 300 is out of the predetermined range through the temperature sensor 321, the controller 500 operates the semiconductor cooler 322, The temperature of the portion 300 is maintained at a constant temperature.

At this time, the semiconductor cooler 322 is also referred to as a thermoelectric cooler or a Peltier cooler and operates with a thermoelectric effect.

The semiconductor cooling element has both sides, and when DC current flows through the element, it transfers heat from one side to the other side, one side becomes cold and the other side becomes hot.

By maintaining the temperature of the reagent injector 300 at a constant temperature using this principle, optimum measurement can always be performed regardless of the measurement area and the measurement site.

Next, the detection sensor 400 detects the amount of chemiluminescence emitted from the urine sample in the dark room 200, and is installed on the other side of the dark room 200.

That is, the detection sensor 400 detects the chemiluminescence amount of sodium (Na) and potassium (K) contained in the urine and detects the ratio of sodium (Na) and potassium (K) will be.

For example, it is possible to detect the potassium (K) content by comparing the photodetection amount sensed by the sensing sensor 400 with the photodetection amount of the predetermined sodium (Na) concentration and inversely calculating the difference therebetween. And potassium (K) in the blood sample to detect the disease.

Hereinafter, the procedure for measuring sodium potassium in the urine using the chemiluminescence measurement device having the above-described configuration will be described.

The user inserts the sample tube into the sample tube holder 240 after taking the urine sample and injecting it into the sample tube.

Next, the opening / closing cover 111 of the case 100 is opened, and the sample tube holder 240 is inserted into the charging port of the dark room 200 through the entrance 110 of the case 100.

At this time, the sample tube holder 240 is coupled to the mounting portion 231 of the rotating body 230 of the dark room 200.

To be precise, the mounting portion 231 is inserted into the coupling groove 242 on the bottom surface of the sample tube holder 240, and the coupling is made between them.

When the insertion of the dark room 200 of the sample tube holder 240 is completed, the opening / closing cover 111 is rotated to close the entrance 110 and rotate the rotating body 230 by 180 degrees.

The rotating body 230 is rotated through a driving shaft 220 and a sample tube holder 240 containing a urine sample is made to correspond to the sensing sensor 400.

At this time, the sample tube in the sample tube holder 240 corresponds to the detection sensor 400 through the detection hole 241.

Next, the reagent is injected into the sample tube for chemiluminescence through chemical reaction of the urine sample.

At this time, each reagent of the reagent injector 300 is injected into each sample tube.

When the reagent is injected into the sample tube as described above, the vibrating means 231a is operated to apply vibration to the sample tube holder 240. [

At this time, as the vibration of the vibration motor 231a is transmitted to the sample tube, mixing between the urine sample and the reagent is efficiently performed in the sample tube.

Thereafter, the urine sample and the reagent chemically react with each other while the chemical reaction occurs, and the detection sensor 400 detects and measures the amount of chemiluminescence generated from each sample.

At this time, the controller 500 compares the predetermined amount of chemiluminescence with the amount of chemiluminescence generated from the urine sample of the user, and displays the ratio of sodium and potassium contained in the urine to the monitor of the measuring device.

That is, when the measured light emission amount is smaller than the predetermined light amount based on the predetermined sodium concentration, it can be seen that the potassium content is higher than that of sodium, and when the measured light amount is larger than the light amount depending on the sodium concentration, It is.

After the series of processes, the control unit 500 converts the measured values into data and displays the sodium and potassium ratio values in the urine to the user, thereby completing measurement of sodium and potassium in the urine.

Thereafter, the user sends the measurement data to the specialist and is followed up by the specialist.

As described above, the apparatus for measuring the amount of chemiluminescence for examining a biomarker according to the present invention is miniaturized in a portable manner so that convenient measurement by a user can be performed regardless of a location. Vibration is applied to a sample tube containing a urine sample, So that it is possible to effectively mix the components.

In addition, there is a technical feature that the reagent injection unit is provided with the constant temperature maintenance means so that the temperature of the reagent can be kept constant regardless of the external environment.

As a result, it is possible to improve the accuracy and reliability of the measurement value in addition to the convenience of the portable type.

In the meantime, the chemiluminescence measuring apparatus for detecting a biomarker disclosed in the present specification may measure chemiluminescence variously using a conjugate which reacts with sodium or potassium from urine of a human body.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is evident that many alternatives, modifications and variations will be apparent to those skilled in the art.

100: Case 110: Entrance
111: opening / closing cover 200: dark room
210: body 210a: concave and convex
211: inlet 212: cover
220: drive shaft 230: rotating body
231: mounting portion 231a: vibration means
240: Sample tube holder 241: Sense hole
242: coupling groove 300: reagent injection part
310: cage 320: means for keeping the temperature constant
321: Temperature sensor 322: Semiconductor cooler
400: detection sensor 500: control unit
510: Timer

Claims (4)

A case having an entrance formed on one side thereof;
A driving shaft provided at a center of the body; a driving shaft rotatably and rotatively driven by the driving shaft; and a sample tube holder mounted on a lower portion of the driving shaft, A dark room including a rotating body provided with a mounting portion;
A reagent injection unit installed at one side of the dark room for injecting a reagent into a sample tube of a sample tube holder;
And a detection sensor installed in a hermetically sealed state with respect to the interior of the dark room body and detecting the amount of chemiluminescence chemiluminescence emitted from the sample tube holder,
Wherein the mounting portion is provided with a vibrating means for applying vibration to the sample tube holder, and a constant temperature maintaining means for keeping the temperature of the reagent constant at all times is provided in the reagent injecting portion. The method according to claim 1,
The above-
A temperature sensor for detecting the temperature of the reagent injecting unit; and a semiconductor cooler for maintaining the constant temperature of the reagent in the reagent injecting unit through temperature sensing by the temperature sensor. 3. The method according to claim 1 or 2,
The sample tube holder is formed to have a curvature corresponding to the curvature of the rotating body, a coupling groove is formed in the bottom surface of the sample tube holder,
Wherein the mounting portion is formed as a protrusion protruding from the bottom of the rotating body so as to be fitted into the coupling groove, and the vibrating means is provided as a vibration motor provided in the mounting portion. 3. The method according to claim 1 or 2,
Wherein the concave and convex portions are formed on the inner circumferential surface of the dark room body along the inner circumferential surface and the concave and convex portions are continuously formed on the upper surface of the rotating body in the concentric circular direction.

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