KR101087720B1

KR101087720B1 - Fluorescence measuring apparatus

Info

Publication number: KR101087720B1
Application number: KR20100004485A
Authority: KR
Inventors: 김경남
Original assignee: (주)마이크로디지탈
Priority date: 2010-01-18
Filing date: 2010-01-18
Publication date: 2011-11-30
Also published as: KR20110084759A

Abstract

The present invention relates to a fluorescence measuring device for measuring the fluorescence of algae contained in a sample, the body; A case protecting the main body; A sample stage installed in the main body and having a sample container (cuvette) for receiving the sample therein; An inspection light irradiation device which is installed in the main body and irradiates inspection light to the sample container; A fluorescence detector installed in the main body and detecting fluorescence emitted from the algae in response to the inspection light; A reagent supply device installed in the main body and supplying a reagent to the sample container; And a control unit for applying a control signal to the inspection light irradiation device, the fluorescence detection device, and the reagent supply device, so that the process is automated and can be used very easily even on the spot during sample collection. It improves the reliability of, can detect various kinds of algae at the same time, and has the effect of further improving the discernment of the result judgment.

Description

Fluorescence measuring apparatus

The present invention relates to a fluorescence measuring device, and more particularly, to measure the fluorescence of various wavelengths generated by microorganisms, plants, crops, phytoplankton, and the like that photosynthesis helps to inspect, research, manage and monitor water quality. It relates to a fluorescence measuring device that can give.

In general, water resources such as rivers, seas, and lakes are seriously threatened by various pollutants, toxic substances, and toxic substances that are incidental to industrial activities and human life.

Therefore, in order to protect such water resources in recent years, the degree of water pollution is progressing, and researches are being actively conducted in various fields in order to determine the degree of various pollutants, harmful substances, and toxic substances on water pollution.

For this study, water quality inspection equipments for essential water quality inspection have been widely developed and used.

In particular, as a kind of water testing equipment, a fluorescence measuring device for measuring the fluorescence of algae contained in a sample has been developed and used.

In general, the fluorescence measuring device, by placing a sample and a reagent in a sample container through a manual operation, and irradiated with light using an LED or the like in the sample container, the fluorescence of a specific wavelength emitted by the algae contained in the sample photosynthesis It is detected by a fluorescence detector.

However, such a conventional fluorescence measuring device was very inconvenient to use immediately when taking a sample in the field because it depends largely on manual work, and the reliability of the result was not reflected because it did not reflect the characteristics of algae which is very sensitive to temperature. There was a problem that it was very falling and was unable to test various kinds of algae at the same time.

An object of the present invention for solving the above problems is to provide a fluorescence measuring device that can be used very immediately at the time of taking the sample outdoors in the field by automating the pretreatment operation such as adding a reagent to the sample inside the body. Is in.

In addition, another object of the present invention is to provide a fluorescence measuring device that can precisely control the temperature of the reagent and the temperature of the sample to improve the reliability of the result, and to obtain the same result regardless of the ambient temperature in the field. have.

In addition, another object of the present invention is to provide a fluorescence measuring device capable of simultaneously detecting various kinds of algae sensitive to a specific wavelength using a multi-wavelength irradiation apparatus.

In addition, another object of the present invention is to provide a fluorescence measuring device that can further enhance the ability of judging result value by promoting photosynthesis of algae using a facilitating light irradiation apparatus.

The fluorescence measuring device of the present invention for achieving the above object, In the fluorescence measuring device for measuring the fluorescence of algae contained in the sample, the body; A case protecting the main body; A sample stage installed in the main body and having a sample container (cuvette) for receiving the sample therein; An inspection light irradiation device which is installed in the main body and irradiates inspection light to the sample container; A fluorescence detector installed in the main body and detecting fluorescence emitted from the algae in response to the inspection light; A reagent supply device installed in the main body and supplying a reagent to the sample container; And a control unit for applying a control signal to the inspection light irradiation device, the fluorescence detection device, and the reagent supply device.

In addition, according to the present invention, the reagent supply device, a reagent injection nozzle for introducing a reagent into the reagent inlet of the sample container; A container holder containing a reagent container in which a reagent is stored; A reagent line connecting the reagent container accommodated in the container receiving container with the reagent injection nozzle; And a reagent pump installed in the reagent line and transferring the reagent of the reagent container to the reagent injection nozzle.

In addition, the fluorescence measuring device of the present invention, it is preferable to further comprise a temperature control device for adjusting the temperature of the reagent and the sample so as to adjust the temperature of the reagent and the sample.

In addition, according to the present invention, the temperature control device, the first heat exchange pocket is installed on the main body, one side of the sample stage; A second heat exchange pocket installed at one side of the main body and installed at one side of the container accommodating container containing the reagent container in which the reagent is stored; A heat medium line for supplying a heat medium to the first heat exchange pocket and the second heat exchange pocket; And a heat medium circulator connected to the heat medium line to circulate the heat medium in the heat medium line; . &Lt; / RTI >

In addition, the fluorescence measuring device of the present invention, is provided in the main body, further comprises a accelerated light irradiation device for irradiating the accelerated light for promoting photosynthesis of algae inside the sample to the sample chamber; The apparatus may be a multi-wavelength irradiation apparatus capable of outputting multi-wavelength inspection light so as to measure various fluorescence of various kinds of birds.

As described above, according to the fluorescence measuring apparatus of the present invention, the process can be automated and can be used very easily at the time of in-field collection of samples, improving the reliability of the result, and detecting various kinds of algae at the same time. It has the effect of further improving the discernment of judgment.

1 is an external perspective view illustrating a fluorescence measuring apparatus according to an exemplary embodiment of the present invention.
2 is an exploded perspective view of the component of FIG. 1.
3 is an enlarged perspective view illustrating an enlarged view of the temperature control device of FIG. 2.
4 is an enlarged perspective view illustrating an enlarged inspection light irradiation device, a fluorescence detection device, and an accelerated light irradiation device of FIG. 3.
5 is a photograph showing a fluorescence measuring device according to another preferred embodiment of the present invention.
6 is a photograph illustrating a state in which the case of FIG. 5 is removed.
FIG. 7 is an enlarged photograph showing the sample stage and the sample container of FIG. 6. FIG.
8 is a diagram illustrating an example of a control screen of a computer connected to the display or the main body of FIG. 5.

Hereinafter, a fluorescence measuring apparatus according to various exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

1 is an external perspective view showing a fluorescence measuring apparatus according to an embodiment of the present invention, Figure 2 is an exploded perspective view of the component of Figure 1, Figure 3 is an enlarged perspective view showing an enlarged temperature control device of Figure 2, 4 is an enlarged perspective view showing an enlarged inspection light irradiation device, a fluorescence detection device, and an accelerated light irradiation device of FIG.

First, as shown in Figures 1 to 4, the fluorescence measuring device according to a preferred embodiment of the present invention, a fluorescence measuring device for measuring the fluorescence of algae contained in the sample, the body 10, the case ( 11), sample stage 20, sample stage transfer device 90, inspection light irradiation apparatus 30, accelerated light irradiation apparatus 40, fluorescence detection apparatus 50, reagent supply apparatus ( 60) and the control part 70 is comprised.

Here, as shown in FIG. 2, the main body 10 includes a sample stage 20, a sample stage transfer apparatus 90, an inspection light irradiation apparatus 30, a accelerated light irradiation apparatus 40, and a fluorescence detection apparatus. (50) It is a kind of main frame which accommodates the reagent supply apparatus 60 and the control part 70.

As shown in FIGS. 1 and 2, the main body 10 is provided with a case 11 for protecting the main body 10, and the case 11 has a sample container 21 and a reagent to be described later. An opening and closing door 12 through which the container 62 may flow in and out may be installed.

In addition, as shown in Figure 3 and 4, the sample stage 20 is installed in the main body 10, the sample container 21 (cuvette) containing the sample is seated, the sample stage transfer It can be transported to the inspection position by the device (90).

5 is a photograph showing a fluorescence measuring apparatus according to another preferred embodiment of the present invention, Figure 6 is a photograph showing a state in which the case of Figure 5 removed.

As described above, the sample stage transfer device 90 may be applied with a wide variety of actuators capable of transferring the sample stage 20 such as a linear motor or a pneumatic / hydraulic cylinder, but is preferably illustrated in FIG. As described above, the sample stage feeder 90 is moved forward and backward by the screw rod 91 and the screw rod 91 rotated by a motor (not shown), and the movable stage on which the sample stage 20 is installed. It is preferable that the stand 92 is included.

Therefore, as shown in Figure 6, when explaining the operation relationship of the sample table transfer device 90, the user opens the opening and closing door 21 of the case 11, the sample container 21 containing the sample, When placed on the sample stage 20 located in the standby position, the movable rod 92 can transfer the sample stage 20 to the test position while the screw rod 91 is rotated by the motor.

Therefore, the sample container 21 seated on the sample stage 20 may be loaded into an inspection position where the inspection light irradiation device 30, the accelerated light irradiation device and the fluorescence detection device 50 are installed, and the inspection may proceed. On the contrary, when the inspection is completed, the movable stand 92 can unload the sample stage 20 to the standby position near the opening / closing door 21.

Here, the sample contained in the sample container 21 is a sewage, waste water, wastewater treatment plant influent, wastewater treatment plant discharge water, underground water, river water, drinking water, sewage or liquid extracted from the soil, gas contaminants adsorbed to the liquid Various samples, such as a dissolved liquid substance, can be used.

3 and 4, the inspection light irradiation device 30 is installed in the main body 10 to irradiate the inspection vessel 21 with the inspection light.

In particular, the inspection light irradiation apparatus 30 is preferably a multi-wavelength irradiation apparatus capable of outputting a multi-wavelength inspection light so as to measure various fluorescence of various kinds of birds.

For example, the inspection light irradiation apparatus 30 may irradiate light having a wavelength of 470 nm, which is a fluorescence characteristic value of green algae, to inspect green algae using a plurality of LEDs, and may be a fluorescence characteristic value of diatoms to inspect diatoms. It is preferable to be able to irradiate light having a wavelength of 520 nm, and to irradiate all of light having a wavelength of 645 nm, which is a fluorescence characteristic value of cyanobacteria, to examine cyanobacteria.

Therefore, various kinds of algae sensitive to a specific wavelength can be easily detected simultaneously or sequentially.

Moreover, the said accelerated light irradiation apparatus 40 is provided in the said main body 10, and irradiates the said sample container 21 with the accelerated light which promotes photosynthesis of the algae inside a sample.

For example, the accelerated light irradiation apparatus 40 is capable of irradiating light having a wavelength of 655 nm to 665 nm in which photosynthesis of all birds occurs most actively.

Thus, by promoting the photosynthesis of algae it is possible to further improve the discernment of the result judgment.

The fluorescence detection device 50 is provided in the main body 10 to detect fluorescence emitted from the algae in response to the inspection light and the facilitation light.

The fluorescence detection device 50 may be provided with an optical sensor capable of detecting a characteristic of fluorescence such as a peak value of fluorescence, and a signal amplifying apparatus for amplifying a detection signal generated by the optical sensor.

Therefore, by detecting the detection signal detected by the fluorescence detection device 50 as a feature it is possible to detect the presence and content of various algae.

On the other hand, as shown in Figure 2, the reagent supply device 60 is installed in the main body 10, and supplies the reagent to the sample container 21, the reagent inlet of the sample container 21 A reagent injection nozzle 61 for introducing a reagent into the container 21a, a container holder 63 for accommodating the reagent container 62 in which the reagent is stored, a reagent container 62 housed in the container holder 63, A reagent pump (65) installed in the reagent line (64) connecting the reagent injection nozzle (61) and the reagent line (64), and transferring the reagent of the reagent container (62) to the reagent injection nozzle (61). It may be made, including.

FIG. 7 is an enlarged photograph showing the sample stage and the sample container of FIG. 6. FIG.

Here, in general, as shown in FIG. 7, the sample container 21 is a container made of a transparent material in which the reagent inlet 21a is opened upward.

In addition, the reagents stored in the reagent vessel 62 may include a pheophytin removal reagent that generally removes pheophytin, a living organism having a chlorophyll-like ecology, in order to measure accurate chlorophyll concentration. Or, it may include a reagent that can remove other unnecessary substances.

Therefore, when explaining the operation relationship of the reagent supply device 60 of the fluorescence measuring device of the present invention, when the user loads the reagent container 62 in the container receiving chamber 63, by the reagent pump 65 Reagents inside the reagent vessel 62 may be automatically supplied to the sample container 21 through the reagent injection nozzle 61 along the reagent line 64.

Therefore, the user can automatically supply the reagent to the sample container 21 without resorting to manual work, and thus can be used very easily on the spot during the field collection of the sample outdoors.

On the other hand, the control unit 70 is the inspection light irradiation device 30, the fluorescence detection device for the automation of the inspection light irradiation device 30, the fluorescence detection device 50 and the reagent supply device 60 described above As a control signal is applied to the 50 and the reagent supply device 60, the control unit 70 may be provided with a display 71.

8 is a diagram illustrating an example of a control screen of a computer connected to the display or the main body of FIG. 5.

As shown in FIG. 8, the control unit 70 selects a frequency at an “A” portion by an automation program, and causes the inspection light irradiation device 30 to emit light having a wavelength of 470 nm, which is a fluorescence characteristic of green algae. In this case, light of 520 nm wavelength, which is the fluorescence characteristic of diatoms, and light of 645 nm, which is the fluorescence characteristic of cyanobacteria, can be irradiated individually or collectively.

On the other hand, as shown in Figure 3, the temperature control device 80, to adjust the temperature of the reagent and the sample to adjust the temperature of the reagent and the sample, is installed in the main body 10, the The first heat exchange pocket 81 is provided on one side of the sample table 20 and the container receiving table 63, which is installed in the main body 10 and accommodates the reagent container 62 in which the reagents are stored. The heat medium line 83 is connected to the heat medium line 83 and the heat medium line 83 for supplying a heat medium to the second heat exchange pocket 82, the first heat exchange pocket 81, and the second heat exchange pocket 82. It may be made by including the heat medium circulator (84) of Figure 1 to circulate the heat medium inside.

Therefore, the operational relationship of the temperature regulating device 80, the heat medium circulated along the heat medium line 83 by the heat medium circulator 84, for example, water, alcohol, oil, etc. The temperature of the sample container 21 seated on the sample stage 20 can be controlled by passing through the heat exchange pocket 81 and passing through the second heat exchange pocket 82. It is possible to adjust the temperature of the reagent vessel 62 seated on the receiving table (63).

In particular, as shown in FIG. 8, the control unit 70 described above causes the temperature control device 80 to select, for example, a temperature at the portion "B" by an automation program, for example, the circulation rate of the heating medium. By increasing the speed or speed of the cooling fan, etc., the temperature of the reagent and the sample can be controlled to be optimally maintained.

Therefore, the temperature of the reagent and the temperature of the sample can be precisely controlled to improve the reliability of the result, and the same result can be obtained regardless of the ambient temperature in the field.

On the other hand, as shown in Figure 8, the above-mentioned control unit 70 instructs the user to automatically load and unload the sample container 21 of the "C" part or supply of reagents by an automated program. It can be.

Therefore, the user can load and unload the sample container 21 or automatically supply reagents to the sample container 21 without having to rely on manual labor. It is.

The present invention is not limited to the above-described embodiments, and of course, modifications may be made by those skilled in the art without departing from the spirit of the present invention.

Therefore, the scope of the claims in the present invention will not be defined within the scope of the detailed description, but will be defined by the following claims and their technical spirit.

10: body 11: case
12: opening and closing door 20: sample stand
21: sample container 21a: reagent inlet
30: inspection light irradiation device 40: accelerated light irradiation device
50: fluorescence detection device 60: reagent supply device
61: reagent injection nozzle 62: reagent container
63: container container 64: reagent line
65: reagent pump 70: control unit
71: display 80: temperature controller
81: first heat exchange pocket 82: second heat exchange pocket
83: heat medium line 84: heat medium cycle
90: sampler transfer device 91: screw rod
92: movable table

Claims

In the fluorescence measuring device for measuring the fluorescence of algae contained in the sample,
main body;
A case protecting the main body;
A sample stage installed in the main body and having a sample container (cuvette) for receiving the sample therein;
An inspection light irradiation device which is installed in the main body and irradiates inspection light to the sample container;
A fluorescence detector installed in the main body and detecting fluorescence emitted from the algae in response to the inspection light;
A reagent supply device installed in the main body and supplying a reagent to the sample container; And
A control unit for applying a control signal to the inspection light irradiation device, the fluorescence detection device, and the reagent supply device;
Fluorescence measuring device comprising a.

The method of claim 1,
The reagent supply device,
A reagent injection nozzle for introducing a reagent into the reagent inlet of the sample container;
A container holder containing a reagent container in which a reagent is stored;
A reagent line connecting the reagent container accommodated in the container receiving container with the reagent injection nozzle; And
A reagent pump installed in the reagent line and transferring the reagent of the reagent container to the reagent injection nozzle;
Fluorescence measuring device comprising a.

The method of claim 1,
And a temperature controller for controlling the temperature of the reagent and the sample to control the temperature of the reagent and the sample.

The method of claim 3, wherein
The temperature control device,
A first heat exchange pocket installed at the main body and installed at one side of the sample stand;
A second heat exchange pocket installed at one side of the main body and installed at one side of the container accommodating container containing the reagent container in which the reagent is stored;
A heat medium line for supplying a heat medium to the first heat exchange pocket and the second heat exchange pocket; And
A heat medium circulator connected to the heat medium line to circulate the heat medium in the heat medium line;
Fluorescence measuring device comprising a.

The method of claim 1,
Promoted light irradiation device which is provided in the main body, for irradiating the accelerated light for promoting photosynthesis of algae inside the sample to the sample container;
The inspection light irradiation apparatus is a fluorescence measuring device, characterized in that the multi-wavelength irradiation apparatus that can output a multi-wavelength inspection light to measure a variety of fluorescence of various kinds of birds.