KR101072826B1 - Composition additives of pork feed and preparation method thereof - Google Patents

Abstract

The present invention relates to a pig feed additive composition and a method for producing the same, and a pig feed additive composition comprising a feed additive composition for pigs and / or a feed additive composition for pigs prepared by the above-mentioned method and a method for producing the same.
Pig feed additive composition comprising pineapple, pears, figs, and rice bran in the broth used as the main ingredients of the pig feed of the present invention is added to the pig feed and ingested such pig feed in pigs to prevent disease, health, etc. By supplying quality pigs to the market, it can contribute to increasing income for pig farmers and improving the health of consumers who eat pork.

Description

Composition additives of pork feed and preparation method thereof

The present invention relates to a pig feed additive composition and a method for producing the same, and a pig feed additive composition comprising a feed additive composition for pigs and / or a feed additive composition for pigs prepared by the above-mentioned method and a method for producing the same.

The main function of livestock products is the aesthetic satisfaction through the supply of nutrients necessary for general life activities, taste, texture and aroma. Recently, however, there is a growing interest in the role of livestock products for improving health-related life functions and the development of livestock products for them. Human health-related theories related to the consumption of foods, including livestock products, have recently been summarized: strengthening immune responses, preventing diseases, treating diseases, balancing rhythms of biorhythms, and delaying aging. In this regard, functional livestock is first defined as "livestock that can exhibit one or more health-related functions in addition to its function as a general nutrient." Therefore, considering both nutrient-related hyperactivity or disease prevention, as well as the possibility of deterioration or even disease-induced functional livestock, functional livestock can be developed not only through specific functional or nutrient-enriched livestock, but also through deterioration of dysfunctional components. have. Functional livestock products that can be produced according to this concept can be classified into three types according to the expression mechanism of the bioactive function.

(1) Functionally-enhanced livestock products: Livestock products with increased, fortified or activated functional ingredients present in general livestock products, for example, increased fatty acids, vitamins, antibodies, proteins, enzymes, antioxidants, etc.

(2) Dysfunctional livestock products: Livestock products that weaken, eliminate, or inactivate health-inhibiting substances or components present in general livestock products, such as livestock products with reduced or eliminated content of cholesterol, fat, saturated fatty acids, odor-causing substances, etc.

(3) Functional production livestock products: In general, functional ingredients or substances that did not exist in livestock products are transferred from outside, deposited, and produced livestock products, such as certain fatty acids, vitamins, minerals, plant extracts, pigment antioxidants, etc. Livestock that has been metastasized or produced.

Here, according to functional components, the accumulation sites in livestock or livestock products and the degree of accumulation by component are as follows. That is, fat-soluble substances such as fatty acids and fat-soluble vitamins accumulate in adipose tissue even in animal products, and vitamins, fatty acids, and minerals accumulate in muscle tissue. In addition, the organs of animals have a high degree of accumulation of minerals such as vitamin A, iron, copper, and chromium. And functional ingredients easily accumulate in livestock or organs are already present in small quantities in existing livestock products. However, in the case of components introduced from the outside, for example, functional flavonoids, pigments, steroids, and rare earths present in plants, the efficiency of transferring them to livestock products through feed is very low at present. In addition, since most of the functional components have different accumulation characteristics in the animal body and livestock products depending on the characteristics of the substance or the type of livestock to be fed, understanding these characteristics helps to improve the production efficiency of functional livestock products.

As the national income increases and the population increases, the demand for animal protein foods such as meat, milk, and eggs rapidly increases, and the number of heads of livestock is also increasing to meet this growing demand. It is well known that feed consumption is also rapidly increasing because the number of heads of livestock is being increased and innovation of the specification technology is performed together.

Feed is essential for livestock raising, accounting for 60-80% of the livestock production costs, making it an important product. Reducing feed costs by making good use of feed contributes to lower livestock production costs, increase international competitiveness of livestock products, and promote consumption, making livestock a major industry.

Since Korea's feed industry began to develop in the 1960s, it has continued to grow and occupy a large part of the Korean economy. Currently, the feed industry is in the midst of industrialization, producing 10 million tons per year. However, most feed materials still depend on imports from foreign countries. There are also many unstable factors such as the need to do it. Considering our reality that almost all of the animal feed, the animal feed required, is dependent on imports, it is very important to save the feed stock and maximize its utilization efficiency.

In addition, the price of domestic feed market is rising sharply in the end of 2008 and the global grain market is facing a sharp rise in 2009. In order to achieve this purpose, first, the nutrient content of various feeds and their value as feed for various livestock must be known correctly. Second, the calculation of feed and the production of formulated feed must be made well. Third, the storage, cooking and processing of feed Understand the technology. Therefore, it is necessary to accurately understand the characteristics and nutritional value of various feeds and improve the utilization rate of nutrients in feed by improving feed processing technology.

The present invention includes a variety of physiologically active substances that have been studied for the functional feed, and in particular, pineapple, pears, figs, and rice bran with various enzyme functions, which have good protein and carbohydrate degradability and help digestion from ancient times. To provide a functional feed additive and a feed comprising such functional feed additive.

Pineapple is succulent, contains about 10% sucrose and 1% citric acid, and has a refreshing sour and sweet taste. Vitamin C is the most fruit and contains 60mg of 100g. It also contains proteolytic enzymes called bromelain to help digest meat.

Pear is 80% of the edible part of the fruit, 85-88% moisture, calories about 50kal. As an alkaline food, the main component is carbohydrate and contains 10 to 13% of sugars (fructose and sucrose), organic acids such as malic acid, tartaric acid and citric acid, vitamin B, vitamin C, fiber, fat and the like. It is effective in bronchial diseases and is good for colds, relieves and asthma, and helps defecation and diuretic effect. It helps to get rid of sputum and cough, to relieve symptoms when you have a sore throat, cold or aching stomach, and to help treat a boil. In addition, it has a detoxification effect that eliminates hangovers. Because it contains the enzyme, you can grind the meat when you lighten it.

Figs contain about 10% of sugar (glucose and fructose) and have a strong sweetness. Organic acids include malic acid and citric acid, as well as benzaldehyde and protease, a protein that is effective in treating cancer. In addition, it is rich in enzymes such as lipase, amylase and oxidase, as well as fiber and protein.

Rice bran is a bark of brown rice, that is, a powder consisting of bran (seeds, seeds and whistle layers) and rice snow. .

Meat meal is called meat meal by degreasing and then crushing meat residues from slaughterhouses, canneries and meat shops. Meat meal is about 50-55% protein and contains a lot of vitamin B such as vitamin B12 and niacin choline. On the other hand, it is rich in calcium, phosphorus and manganese. However, in spite of these advantages of meat meal, the digestion and absorption rate in the livestock digester is very low due to the low degradation of protein.

It is an object of the present invention to provide a feed additive composition for a swine and a method for preparing the same.

It is another object of the present invention to provide a pig feed composition and a method for preparing the same, including a feed additive composition for pigs and / or a feed additive composition for pigs prepared by the above-mentioned method.

The present invention is a feed additive composition for pigs comprising 5 to 20 parts by weight of pineapple, 5 to 20 parts by weight, 5 to 20 parts by weight of figs, 5 to 20 parts by weight of rice bran, and a method for preparing the same, based on 100 parts by weight of meat meal. Can be provided.

The present invention is a pig feed or pig feed manufacturing method, the pig feed additive composition for the pig feed in the pig feed by 5-20% by weight relative to the total weight of the pig feed or by the method of producing a pig feed additive composition mentioned above in the pig feed It is possible to provide a pig feed comprising the prepared feed additive composition for a pig feed composition 5 to 20% by weight relative to the total weight of the pig feed and a preparation method thereof.

Pig feed additive composition comprising pineapple, pears, figs, and rice bran in the broth used as the main ingredients of the pig feed of the present invention is added to the pig feed and ingested such pig feed in pigs to prevent disease, health, etc. By supplying quality pigs to the market, it can contribute to increasing income for pig farmers and improving the health of consumers who eat pork.

The present invention shows a feed additive composition for pigs and a method for producing the same.

The present invention shows a feed additive composition for a pig for improving the functionality of the pig feed by adding to the pig feed and a method for producing the same.

The present invention shows a feed additive composition for pigs comprising 5 to 20 parts by weight of pineapple, 5 to 20 parts by weight of pears, 5 to 20 parts by weight of figs, and 5 to 20 parts by weight of rice bran.

This invention shows the manufacturing method of the feed additive composition for pigs which mixes and mixes 5-20 weight part of pineapples, 5-20 weight part of pears, 5-20 weight part of figs, and 5-20 weight part of rice bran with respect to 100 weight part of meat meal.

Meat meal (Meat Meal) in the slaughterhouse, canning factory and / or meat debris from the meat factory can be used after degreasing and dried.

Degreasing, drying, and grinding of the meat residues in the above-mentioned meat meal may be performed by a person skilled in the art by a conventional method or a method well known in the art, and thus the detailed description thereof will be omitted.

Pineapple, pears, figs, and rice bran in the above can be used in the form of powder each powdered by a grinder.

Pineapple, pears, figs, and rice bran may be used in the form of powder powdered to have a particle size of 0.1 to 0.5 mm.

Pineapple, pear, fig, and rice bran may be used in the form of powder powdered to have a particle size of 0.3 ± 0.05 mm in each grinder.

In the above pile apple, pear, figs can be used in the form of juice to juice them.

Pig feed additive composition of the present invention, pineapple, pears, figs, rice bran may be further added to certain components in order to further improve the functionality.

The predetermined component may be used rowan.

The predetermined component may use a sweet smell.

The predetermined component may be used in the form of powdered powder after steaming and cooling with steam.

The predetermined component may be used in the form of powder which is steamed at 90 to 100 ° C. for 10 to 24 hours, cooled to room temperature, and then particle size is 0.1 to 0.5 mm.

The predetermined component may be used in the form of powder which is steamed at 100 ° C. for 24 hours, cooled to room temperature, and then particle size is 0.3 mm ± 0.05 mm.

The said predetermined component can use 3-10 weight part with respect to 100 weight part of meat meal.

In another embodiment of the present invention, pig feed comprising 5-20 parts by weight of pineapple, 5-20 parts by weight of pears, 5-20 parts by weight of figs, 5-20 parts by weight of rice bran, and 3-10 parts by weight of rowan The additive composition is shown.

In another embodiment of the present invention, 5 to 20 parts by weight of pineapple, 5 to 20 parts by weight of pears, 5 to 20 parts by weight of figs, 5 to 20 parts by weight of rice bran, and 3 to 10 parts by weight of sweet persimmon, based on 100 parts by weight of meat, Pig feed additive composition is shown.

In another embodiment, the present invention is added with 5 to 20 parts by weight of pineapple, 5 to 20 parts by weight of pears, 5 to 20 parts by weight of figs, 5 to 20 parts by weight of rice bran, and 3 to 10 parts by weight of rowan, based on 100 parts by weight of meat. The manufacturing method of the feed additive composition for pigs is shown.

In another embodiment of the present invention, 5 to 20 parts by weight of pineapple, 5 to 20 parts by weight of pears, 5 to 20 parts by weight of figs, 5 to 20 parts by weight of rice bran, and 3 to 10 parts by weight of sweet persimmon are added to 100 parts by weight of meat. The manufacturing method of the feed additive composition for pigs to mix | blend is shown.

The present invention shows a pig feed comprising a feed additive composition for a pig and a method for producing the same.

The present invention shows a pig feed and improved manufacturing method of the functionality of a pig feed, including a feed additive composition for pigs.

The present invention provides a swine feed and a method of preparing the same, so that the pig feed additive composition prepared by the above-mentioned swine feed additive composition and / or the above-mentioned swine feed additive composition prepared by the above method. Indicates.

The present invention provides a pig feed additive composition prepared by the method of producing a pig feed additive composition mentioned above 5 to 20% by weight relative to the total weight of the pig feed and / or the above-mentioned pig feed additive composition. A pig feed comprising 5 to 20% by weight relative to the total weight of the pig feed is shown.

The present invention provides a method of producing a pig feed, the pig feed additive composition mentioned above in a pig feed prepared by 5 to 20% by weight relative to the total weight of the pig feed and / or the method of producing a feed additive composition for pigs mentioned above. A method of producing a pig feed is shown by adding and mixing 5 to 20% by weight of a feed composition for a pig feed with respect to the total weight of the pig feed.

Pig feed additive composition of the present invention and a method for producing the same and a pig feed and a method for producing the same under various conditions, in order to achieve the object of the present invention, a feed additive composition for a pig and a method for producing the same under the above-mentioned conditions and It is desirable to provide a pig feed and a method of making the same.

Hereinafter, the content of the present invention will be described in detail through experimental examples, examples and test examples. However, these are for the purpose of illustrating the present invention in more detail, and the scope of the present invention is not limited thereto.

Experimental Example 1 Analysis of Mineral Components of Pineapple, Fig, Pear, Rice Bran, and Meat Powder

ICP-AES measurement conditions in Table 1 (model name: OPTIMA 4300 DV, manufacturer: PerkinElmer, measurement elements: Ca, K, Mg, Na, P, Fe, Mn, Zn) and ICP-MS measurement conditions in Table 2 (model name: X series, manufacturer: Thermoelemental, measuring elements: Cu, Se, Ge, Cd) was used to measure the mineral components for pineapple, fig, pear, rice bran and meat meal and the results are shown in Tables 3 to 7 below. .

In the above, pineapple, fig, pear, rice bran and ground meat were used as samples, each of which was ground to a size of 0.3mm.

ICP-AES Measurement Conditions Measuring conditions R.F. Frequency 40.68 MHz R.F. Power 1300 W Coolant Gas Flow 15 L / min Auxiilary Gas Flow 0.2 L / min Nebulizer gas flow 0.7 L / min

ICP-MS Measurement Conditions Measuring conditions R.F. Frequency 27.12 MHz R.F. Power 1300 W Coolant Gas Flow 13 L / min Auxiilary Gas Flow 0.9 L / min Nebulizer gas flow 0.7 L / min Sampler Cone Ni Skimmer cone Ni Analytical Mass (Au) 197

Mineral Ingredients of Pineapple Kinds Ca K Mg Na P Fe Mn Zn Cu Se Ge CD pineapple 98.4 1335 115.8 4.3 50.5 1.0 10.1 1.1 0.6 - - -

* "-" In Table 3 means not measured.

Mineral Ingredients of Figs Kinds Ca K Mg Na P Fe Mn Zn Cu Se Ge CD FIG 379.7 1857 129.1 31.7 106 3.7 0.6 1.0 0.1 - - -

* In Table 4, "-" means not measured.

Pear Mineral Ingredients Kinds Ca K Mg Na P Fe Mn Zn Cu Se Ge CD ship 15.8 1126 71.3 7.6 80.2 0.2 0.7 0.7 0.4 - - -

* "-" In Table 5 means that it was not measured.

Mineral component of rice bran Kinds Ca K Mg Na P Fe Mn Zn Cu Se Ge CD Rice bran 831 14339 7239 105.0 30549 85.7 109.2 53.1 5.2 0.38 0.022 -

* "-" In Table 6 means that it was not measured.

Sextant mineral Kinds Ca K Mg Na P Fe Mn Zn Cu Se Ge CD Six minutes 61932 5142 1838 33.9 28850 133.8 3.3 52.6 4.2 0.53 0.013 -

* "-" In Table 7 means that not measured.

Experimental Example 1 Analysis of Vitamin Components of Pineapple, Fig, Pear, Rice Bran, and Meat Powder

To compare the vitamin content and content of pineapple, fig, pear, rice bran and meat meal, 1 gram of each sample of pineapple, fig, pear, rice bran and meat meal was processed according to the micronutrient test method of the Food Code. Analyzed by HPLC (waters 510).

HPLC conditions for measuring the vitamin component and content of pineapple, fig, pear, rice bran and meat meal were performed as shown in Tables 8 to 19 below. At this time, the flow rate was 30 ml / hr of solvent, 20 ml / hr of ninhydrin, and pressure was 55 bar of solvent and 12 bar of ninhydrin.

Meanwhile, the content of vitamin components for pineapple, pear, fig, rice bran, and meat meal using HPLC (waters 510) system are shown in Tables 20 to 24.

Vitamin Content of Pineapple Kinds A
(Μ / 100 g, RE) B1
(Mg / 100g) B2
(Mg / 100g) B6
(Mg / 100g) B12
(Μg / 100g) C
(Mg / 100g) D3
(Μg / 100g) E
(Mg / 100g, α-TE) K1
(Μg / 100g) Niacin
(Mg / 100g, NE) Pantothenic acid
(Mg / 100g) Biotin
(Μg / 100g) Folic acid
(Μg / 100g) beta
carotene
(Μg / 100g) pineapple - 1.26 - - - - - - - 0.53 - 744.56 - - Fat soluble & water soluble G Number Number Number Number Number G G G Number Number Number Number G

Vitamin content of figs Kinds A
(Μ / 100 g, RE) B1
(Mg / 100g) B2
(Mg / 100g) B6
(Mg / 100g) B12
(Μg / 100g) C
(Mg / 100g) D3
(Μg / 100g) E
(Mg / 100g, α-TE) K1
(Μg / 100g) Niacin
(Mg / 100g, NE) Pantothenic acid
(Mg / 100g) Biotin
(Μg / 100g) Folic acid
(Μg / 100g) beta
carotene
(Μg / 100g) FIG - - - - - - - 0.7742 44.7794 0.16 - 51.86 - - Fat soluble & water soluble G Number Number Number Number Number G G G Number Number Number Number G

Vitamin content of pears Kinds A
(Μ / 100 g, RE) B1
(Mg / 100g) B2
(Mg / 100g) B6
(Mg / 100g) B12
(Μg / 100g) C
(Mg / 100g) D3
(Μg / 100g) E
(Mg / 100g, α-TE) K1
(Μg / 100g) Niacin
(Mg / 100g, NE) Pantothenic acid
(Mg / 100g) Biotin
(Μg / 100g) Folic acid
(Μg / 100g) beta
carotene
(Μg / 100g) ship - - - - - - - 0.4459 - - - - - - Fat soluble & water soluble G Number Number Number Number Number G G G Number Number Number Number G

Vitamin Content of Rice Bran Kinds A
(Μ / 100 g, RE) B1
(Mg / 100g) B2
(Mg / 100g) B6
(Mg / 100g) B12
(Μg / 100g) C
(Mg / 100g) D3
(Μg / 100g) E
(Mg / 100g, α-TE) K1
(Μg / 100g) Niacin
(Mg / 100g, NE) Pantothenic acid
(Mg / 100g) Biotin
(Μg / 100g) Folic acid
(Μg / 100g) beta
carotene
(Μg / 100g) Rice bran - 1.03 - - - - 45.3922 5.9927 - 1.96 - 234.03 - 75.1671 Fat soluble & water soluble G Number Number Number Number Number G G G Number Number Number Number G

Vitamin content of meat Kinds A
(Μ / 100 g, RE) B1
(Mg / 100g) B2
(Mg / 100g) B6
(Mg / 100g) B12
(Μg / 100g) C
(Mg / 100g) D3
(Μg / 100g) E
(Mg / 100g, α-TE) K1
(Μg / 100g) Niacin
(Mg / 100g, NE) Pantothenic acid
(Mg / 100g) Biotin
(Μg / 100g) Folic acid
(Μg / 100g) beta
carotene
(Μg / 100g) Six minutes - - - - - - - - - 6.43 - - - - Fat soluble & water soluble G Number Number Number Number Number G G G Number Number Number Number G

* In Tables 20 to 24, "-" means not measured, "lip" in the fat-soluble & water-soluble item means a fat-soluble vitamin, "water" means a water-soluble vitamin.

As a result of the analysis of the vitamin components of pineapple, fig, pear, rice bran and meat powder as shown in Tables 20 to 24, it was found that the vitamin content of meat powder was the lowest, and the vitamin and rice bran contained various vitamin components.

Experimental Example 2 Analysis of Amino Acid Content of Pineapple, Fig, Pear, Rice Bran, and Meat Meal

In order to analyze the amino acid content in pineapple, fig, pear, rice bran and meat meal, 20 ml of distilled water was added to 1 g of each sample powder for pineapple, fig, pear, rice bran and meat meal, and then heated at 80 ° C. for 2 hours and cooled at room temperature. After centrifugation for 15 minutes at 3000rpm to recover the supernatant. The recovered supernatant was washed with the same amount of chloroform and centrifuged at 3000 rpm for 15 minutes to separate the supernatant. The supernatant was derivatized with phenyl isothio cyanate (PITC) according to the pico-tag method, and 20 μl of the supernatant was analyzed by HPLC. The HPLC operating conditions such as HPLC analysis equipment and HPLC analysis conditions are shown in Tables 25 to 28 below. Indicated.

Meanwhile, the amino acid contents of pineapple, pear, fig, rice bran, and meat meal using HPLC are shown in Table 29 and Table 30.

HPLC analysis equipment for measuring free amino acids a. System: 2 Waters 510 HPLC Pumps
Waters gradient controller
Waters 717 Automatic sampler b. Column: Waters Pico-tag column (3.9 × 300㎜, 4㎛) c. Detector: Waters 2487 UV detector, 254nm d. Data analysis empower 2 software

HPLC analysis conditions for the determination of free amino acids Time Flow % A % B Initial 1.0 100 0 9.0 1.0 86 14 9.2 1.0 80 20 17.5 1.0 54 46 17.7 1.0 0 100 18.2 1.0 0 100 20.0 1.0 0 100 20.7 1.0 0 100 21.0 1.0 100 0 24.0 1.0 100 0 25.0 1.0 100 0

In Table 26,% A is 140 mM sodium acetate (6% acetonitrile) and% B is 60% acetonitrile.

HPLC analysis equipment for measuring total amino acid a. System: 2 Waters 510 HPLC Pumps
Waters gradient controller
Waters 717 Automatic sampler b. Column: Waters Pico-tag column (3.9 × 300㎜, 4㎛) c. Detector: Waters 2487 UV detector, 254nm d. Data analysis empower 2 software

HPLC Analysis Conditions for Determination of Total Amino Acids Time Flow % A % B Initial 1.0 100 0 9.0 1.0 86 14 9.2 1.0 80 20 17.5 1.0 54 46 17.7 1.0 0 100 18.2 1.0 0 100 20.0 1.0 0 100 20.7 1.0 0 100 21.0 1.0 100 0 24.0 1.0 100 0 25.0 1.0 100 0

In Table 28,% A is 140 mM sodium acetate (6% acetonitrile) and% B is 60% acetonitrile.

Free amino acid content (mg / 100mg) of pineapple, pear, fig, rice bran and meat Kinds pineapple FIG ship Rice bran Six minutes Asp 0.041 0.075 0.228 0.019 0.009 Glu 0.070 0.032 0.021 0.043 0.027 Asn 0.084 0.958 0.560 0.028 0.003 Ser 0.080 1.374 0.122 0.008 0.009 Gln 0.026 0.111 - 0.002 0.002 Gly 0.021 0.019 - 0.006 0.020 His 0.048 0.028 - 0.008 0.014 Arg 0.034 0.037 - 0.015 0.092 Thr 0.019 0.033 0.007 0.004 0.008 Ala 0.063 0.142 0.025 0.019 0.043 Pro 0.011 0.077 0.050 0.001 0.013 Tyr 0.059 0.037 0.028 0.005 0.007 Val 0.032 0.034 0.038 0.004 0.013 Met 0.024 0.007 0.007 - 0.003 Cys2 - - - 0.001 -

Total amino acid content (mg / 100mg) of pineapple, pear, fig, rice bran and meat Kinds pineapple FIG ship Rice bran Six minutes Asp 0.147 0.581 0.538 0.447 0.466 Glu 0.251 0.333 0.119 0.924 1.523 Ser 0.138 0.199 0.146 0.532 0.569 Gly 0.192 0.211 0.084 0.755 2.935 His 0.044 0.057 0.024 0.348 0.517 Arg 0.036 0.054 0.024 0.361 1.513 Thr 0.087 0.112 0.068 0.635 0.758 Ala 0.154 0.284 0.079 0.561 1.505 Pro 0.193 0.280 0.106 2.449 2.274 Tyr 0.015 0.014 0.023 0.087 0.386 Val 0.162 0.193 0.095 0.825 1.129 Met 0.018 0.016 0.020 0.113 0.359 Cys2 0.005 0.002 0.017 0.045 0.047 Ile 0.119 0.121 0.061 0.447 0.789 Leu 0.149 0.165 0.083 0.728 1.441 Phe 0.065 0.056 0.034 0.706 0.915 Trp 0.002 0.001 0.002 - 0.019 Lys 0.040 0.064 0.009 0.063 0.919 Total 1.817 2.743 1.532 9.846 18.062

* "-" In Table 29 and Table 30 means that not measured.

As shown in Table 29 and Table 30, the pineapple, fig, pear, rice bran, and meat meal were found to contain various kinds of amino acids.

Experimental Example 3 Determination of Enzyme Capacity of Pineapple, Fig, and Pear

Enzyme activity of alpha-amylase and protease for pineapple, fig and pear were measured by the following analytical methods (a) and (b), and the results are shown in Table 31 below. .

(A) Alpha-amylase analysis method of pineapple, pear, fig

(1) Dilute the sample 10 times with 0.1% NaCl solution.

(2) 5 ml of 1% soluble starch + 3 ml of Macllvain buffer solution (pH 6.0) + 1 ml of CaCl ₂ and left at 37 ° C. for 5 minutes → 1 ml of sample solution → 37 ° C., left for 30 minutes

(3) 0.2 ml of reaction solution + 10 ml of iodine solution

(4) 660 nm wavelength absorbance measurement

(5) OD ₀ : enzyme activity inhibitory control, OD ₁ : treatment, OD _ST : distilled water treatment

(6) Digested starch content (Unit / g) = ((OD ₀ -OD ₁ ) / OD _ST ) × dilution factor

* When 1 mg of starch is digested every 30 minutes, 1 unit is used.

(B) Protease analysis method of pineapple, pear, fig

(1) Dilute the sample 10 times with 0.1% NaCl solution.

(2) 1 ml of 0.6% casein (pH 6.0) + 1 ml of sample solution → 37 ° C. for 10 minutes

(3) Mix 2 ml of 0.4M TCA solution → reaction at 37 ℃ for 20 minutes (stop reaction)

(4) 1 ml of filtrate + 5 ml of Na ₂ CO ₃ + 1 ml of polyline solution → color development (20 minutes)

(5) 660 nm wavelength absorbance measurement

(6) Unit / g = ((EE ₀ ) × 4 × dilution factor

E: treatment, E ₀ : control, E _S : standard solution

* 10 minutes, the titer to produce nonproteinaceous substances corresponding to 1 mg of tyrosine is 1 unit

Enzyme Activity of Pineapple, Pears, and Figs Item α-Amylase Protease FIG 0.1 0.6 pineapple 1.8 1.8 ship - 0.07

* "-" In Table 31 means not measured.

≪ Example 1 >

5 parts by weight of pineapple, 5 parts by weight of pears, 5 parts by weight of figs, and 5 parts by weight of rice bran were added and blended to prepare a feed additive composition for pigs.

The meat meal (Meat Meal) was used to collect only the meat residues from the slaughterhouse, cannery or meathouse, then degreased and dried at 110 ℃ for 1 hour and then ground to a particle size of 0.3 ± 0.05mm.

In the above pineapple, pears, figs, rice bran was used to powder the powder so that the particle size of 0.3 ± 0.05mm respectively.

<Example 2>

10 parts by weight of pineapple, 10 parts by weight of pears, 10 parts by weight of figs, and 10 parts by weight of rice bran were added and blended to prepare a feed additive composition for swine.

In the above meal, only the meat residues from the slaughterhouse, cannery or meathouse were collected, degreased, dried at 110 ° C. for 1 hour, and then ground to a particle size of 0.3 ± 0.05 mm.

In the above pineapple, pears, figs, rice bran was used to powder the powder so that the particle size of 0.3 ± 0.05mm respectively.

<Example 3>

15 parts by weight of pineapple, 15 parts by weight of pears, 15 parts by weight of figs, and 15 parts by weight of rice bran were added and blended to prepare a feed additive composition for pigs.

In the above meal, only the meat residues from the slaughterhouse, cannery or meathouse were collected, degreased, dried at 110 ° C. for 1 hour, and then ground to a particle size of 0.3 ± 0.05 mm.

In the above pineapple, pears, figs, rice bran was used to powder the powder so that the particle size of 0.3 ± 0.05mm respectively.

<Example 4>

20 parts by weight of pineapple, 20 parts by weight of pears, 20 parts by weight of figs, and 20 parts by weight of rice bran were added and blended to prepare a feed additive composition for pigs.

In the above meal, only the meat residues from the slaughterhouse, cannery or meathouse were collected, degreased and dried at 110 ° C. for 1 hour, and then ground to a particle size of 0.3 ± 0.05 mm.

In the above pineapple, pears, figs, rice bran was used to powder the powder so that the particle size of 0.3 ± 0.05mm respectively.

Example 5

15 parts by weight of pineapple, 15 parts by weight of pears, 15 parts by weight of figs, 15 parts by weight of rice bran, and 5 parts by weight of rowan were added and blended to prepare a feed additive composition for pigs.

In the above meal, only the meat residues from the slaughterhouse, cannery or meathouse were collected, degreased, dried at 110 ° C. for 1 hour, and then ground to a particle size of 0.3 ± 0.05 mm.

In the above pineapple, pears, figs, rice bran was used to powder the powder so that the particle size of 0.3 ± 0.05mm respectively.

The ash was steamed for 24 hours in a steam of 100 ℃ and then cooled to room temperature and then used a powder powdered to a particle size of 0.3mm ± 0.05mm.

<Example 6>

15 parts by weight of pineapple, 15 parts by weight of pears, 15 parts by weight of figs, 15 parts by weight of rice bran, and 5 parts by weight of sweet persimmon were added and blended to prepare a feed additive composition for pigs.

In the above meal, only the meat residues from the slaughterhouse, cannery or meathouse were collected, degreased, dried at 110 ° C. for 1 hour, and then ground to a particle size of 0.3 ± 0.05 mm.

In the above pineapple, pears, figs, rice bran was used to powder the powder so that the particle size of 0.3 ± 0.05mm respectively.

In the above, the sensational fragrance was steamed for 24 hours with steam at 100 ° C. and then cooled to room temperature.

Application Example 1 Pig feed containing pig feed additive composition

Swine feed was prepared by combining 5% by weight of the feed additive composition for swine feed obtained in Example 1 with 95% by weight of conventional feed for pigs.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

<Application Example 2> Pig feed containing pig feed additive composition

Swine feed was prepared by blending 5% by weight of the feed additive composition for swine feed obtained in Example 2 with 95% by weight of conventional pig feed.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

Application Example 3 Pig feed containing pig feed additive composition

Swine feed was prepared by blending 5% by weight of the feed additive composition for swine feed obtained in Example 3 to 95% by weight of conventional pig feed.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

Application Example 4 Pig feed containing pig feed additive composition

A pig feed was prepared by combining 95% by weight of conventional feed for pigs with 5% by weight of feed additive composition for pigs obtained in Example 4.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

<Application Example 5> Pig feed containing pig feed additive composition

Swine feed was prepared by blending 5% by weight of the feed additive composition for swine feed obtained in Example 5 to 95% by weight of conventional pig feed.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

Application Example 6 Pig feed containing pig feed additive composition

Swine feed was prepared by combining 5% by weight of the feed additive composition for pigs obtained in Example 6 with 95% by weight of the conventional feed for pigs.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

<Application Example 7> Pig feed containing pig feed additive composition

Swine feed was prepared by combining 10% by weight of the feed additive composition for pigs obtained in Example 1 with 90% by weight of the conventional feed for pigs.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

Application Example 8 Pig feed containing pig feed additive composition

Swine feed was prepared by combining 10% by weight of the feed additive composition for swine feed obtained in Example 2 to 90% by weight of the conventional feed for pigs.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

Application Example 9 Pig feed containing pig feed additive composition

Swine feed was prepared by combining 10% by weight of the feed additive composition for swine feed obtained in Example 3 to 90% by weight of the conventional feed for pigs.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

Application Example 10 Pig feed containing pig feed additive composition

A pig feed was prepared by combining 10% by weight of a feed composition for a pig feed additive obtained in Example 4 with 90% by weight of a conventional pig feed.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

Application Example 11 Pig feed containing pig feed additive composition

A pig feed was prepared by blending 10 wt% of a feed additive composition for a swine obtained in Example 5 to 90 wt% of a conventional pig feed.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

Application Example 12 Pig feed containing pig feed additive composition

A pig feed was prepared by combining 10 wt% of a feed additive composition for a swine obtained in Example 6 to 90 wt% of a conventional pig feed.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

Application Example 13 Pig feed containing pig feed additive composition

A pig feed was prepared by combining 15% by weight of the feed additive composition for pigs obtained in Example 1 with 85% by weight of conventional pig feed.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

Application Example 14 Pig feed containing pig feed additive composition

A pig feed was prepared by combining 15% by weight of a feed composition for a pig feed additive obtained in Example 2 with 85% by weight of a conventional pig feed.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

<Application Example 15> Pig feed containing pig feed additive composition

A pig feed was prepared by combining 15% by weight of a feed composition for a pig feed additive obtained in Example 3 with 85% by weight of a conventional pig feed.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

Application Example 16 Pig feed containing pig feed additive composition

A pig feed was prepared by combining 15% by weight of a feed composition for a pig feed additive obtained in Example 4 with 85% by weight of a conventional pig feed.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

Application Example 17 Pig feed containing pig feed additive composition

A pig feed was prepared by combining 15% by weight of a feed composition for a pig feed additive obtained in Example 5 with 85% by weight of a conventional pig feed.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

Application Example 18 Pig feed containing pig feed additive composition

A pig feed was prepared by combining 15% by weight of the feed additive composition for pigs obtained in Example 6 with 85% by weight of conventional pig feed.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

Application Example 19 Pig feed containing pig feed additive composition

A pig feed was prepared by combining 20% by weight of the feed additive composition for pigs obtained in Example 1 with 80% by weight of conventional pig feed.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

Application Example 20 Pig feed containing pig feed additive composition

A pig feed was prepared by blending 20 wt% of a feed additive composition for a swine obtained in Example 2 with 80 wt% of a conventional pig feed.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

Application Example 21 Pig feed containing pig feed additive composition

A pig feed was prepared by combining 20 wt% of a feed additive composition for a swine obtained in Example 3 with 80 wt% of a conventional pig feed.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

Application Example 22 Pig feed containing pig feed additive composition

A pig feed was prepared by blending 20 wt% of a feed additive composition for a swine obtained in Example 4 with 80 wt% of a conventional pig feed.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

Application Example 23 Pig feed containing pig feed additive composition

A pig feed was prepared by blending 20 wt% of a feed additive composition for a swine obtained in Example 5 to 80 wt% of a conventional pig feed.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

Application Example 24 Pig feed containing pig feed additive composition

Swine feed was prepared by combining 20% by weight of the feed additive composition for pigs obtained in Example 6 with 80% by weight of the conventional feed for pigs.

In the above conventional pig feed was used probital feed (Bukkuk feed company).

Test Example 1 Protein Content of Pig Stool

1 to 4 weeks after the pigs ingested the pig feed containing the feed additive composition for the pigs of Application Examples 1 and 2 and the pigs ingested only the conventional pig feed (Probital, Buguk Feed Co.). Protein content of the pig stool was measured using the following method and the results are shown in Table 32 below.

(1) reagents and reagents

A) sulfuric acid

B) Degradation accelerators (Kjeltabs)

0.1N hydrochloric acid

D) Boric acid solution: 1% boric acid solution prepared by adding 100 g of H3BO3, 100 mL of bromocresol green solution and 100 mL of 0.1% methylet solution and applying 10 L

E) sodium hydroxide solution: 20%

(2) Preparation of test solution (decomposition process)

A) Take 1g-5g (containing 3-25% protein) of the sample accurately and place it in the digestion tube and add 2 tablets of the decomposition promoter. Degradation accelerators are decomposed efficiently when the ratio of H ₂ SO ₄ to K ₂ SO ₄ is 1.4∼2.0: 1.

B) Add 12 mL of concentrated sulfuric acid to the digestion tube. If the sample's fat content is 10% or more, add 15 mL of concentrated sulfuric acid.

C) Decompose for 45 ~ 60 minutes in the decomposition device at 420 ℃, and when the color of decomposition liquid becomes transparent light blue, cool it to room temperature.

(3) Test operation (distillation and titration)

In the case of automatic equipment, the following distillation, titration and calculation processes are all performed automatically.

A) Carefully add 80 mL of distilled water to the decomposed test solution.

B) Put the collection solution containing 25mL mixed indicator into the Erlenmeyer flask, place it on the distillation apparatus and lift the Erlenmeyer flask support. During distillation, distillate enters the collection solution.

C) Add 50 mL of 40% NaOH (4 times the amount of sulfuric acid used for decomposition) into the digestion tube.

D) Distill for 3 to 4 minutes in the distillation apparatus. The collection solution in the Erlenmeyer flask of the distillation apparatus turns green while collecting the alkali (amonia) contained in the distillate.

E) Record the amount of acid used for titration until the end point reaches pale pink color using hydrochloric acid solution (0.1N).

Crude protein (%) = [{HCl consumption (ml)-blank test (ml)} × M × 1.401 / sample amount (mg)] × F × 100

 1.401: atomic weight of nitrogen

 M: molarity of HCl

F: Kjeldahl coefficient (6.25)

Protein Content of Swine Stools Item SA (g) Protein count protein(%) Stool after 1 week in piglet fed 1.08 6.25 3.563 Stool after 2 weeks 1.09 6.25 5.967 Stool after 3 weeks of probital pig 1.01 6.25 7.097 Stool after 4 weeks of probital pig 1.10 6.25 5.167 Application Example 1 Stool after 2 weeks of feeding pig 1.18 6.25 5.909 Application Example 1 Stool after 4 weeks of feeding pigs 1.07 6.25 6.958 Application Example 2 Stool after 2 weeks of feeding pig 1.02 6.25 7.428 Application Example 2 Stool after 4 weeks of feeding pigs 1.12 6.25 8.041

As shown in Table 32, the pigs ingesting the pig feed (Application Examples 1, 2) containing the feed additive composition for the pigs of the present invention showed a higher protein content than the pigs ingesting only the pig feed in the related art.

Test Example 2 Measurement of Growth Status of Pigs

Growth of pigs ingested pig feed containing pig feed additive compositions of Application Examples 1, 2, 5 and 6 and Pigs fed only conventional pig feed (Probital, Bubuk feed) A test was conducted to determine the condition.

30 pigs of the same weight were divided into 5 groups of 10 dogs, and then each group was fed a conventional pig feed for 4 weeks (group 1). Piglet containing feed composition for pigs (group 2), pig feed containing pig feed additive composition of Application Example 2 for 4 weeks, piglet containing feed additive composition for pigs, application sample 5, ingested for 4 weeks The growth status of the pig weight gain was measured for the test group (group 4) fed the feed for 4 weeks and the test group (group 5) fed the pig feed containing the feed additive composition for application of pigs of Application Example 6 for 4 weeks. And the results are shown in Tables 33 to 37 below.

Changes in Weight of Pigs Consuming Pig Feed pig
Week 0 One 2 3 4 One 8 11.5 14.4 18.6 22.5 2 7.6 11.6 15.8 19.3 23.5 3 8.2 12.2 16.4 19.9 23.4 4 9 13.2 17.2 20.5 244 5 8.6 12.1 15.6 19.3 22.7 6 7.8 12 15.8 19.9 23.5 7 7.4 11.2 15.1 18.6 22.3 8 6.8 10.8 14.7 18.9 22.6 9 8.2 11.7 15.7 19.4 23.6 10 6.4 10.6 14.6 18.1 21.8 Average 7.8 11.69 15.53 19.25 23.03

Changes in weight of pigs fed pig feed containing pig feed additive composition of Application Example 1 pig
Week 0 One 2 3 4 One 8.5 12.2 16.2 20.4 23.9 2 8.3 11.8 15.9 19.6 23.6 3 8.5 12 16.1 19.6 23.7 4 8.3 11.8 15.7 19.2 22.8 5 8.5 12.2 16.4 20.3 23.8 6 7.9 12.1 16.3 20.2 24 7 8.4 12.1 14 19.8 23.3 8 10 14.1 18 21.6 25.8 9 8.2 12.4 15.9 19.5 23 10 8.4 12.6 16.1 20.1 23.9 Average 8.5 12.33 16.26 20.03 23.78

Changes in weight of pigs fed pig feed containing pig feed additive composition of Application Example 2 pig
Week 0 One 2 3 4 One 8 12.2 15.7 19.2 23.2 2 7.6 11.6 15.1 18.6 22.6 3 8.2 12.2 16.1 19.8 23.3 4 9 13 17.2 21.1 24.6 5 8.6 12.1 16.1 20 23.9 6 7.8 11.8 15.3 18.8 22.8 7 7.4 11.2 15.4 19.3 22.8 8 6.8 10.3 14.3 18.2 22 9 8.2 12.2 16.1 19.1 23.8 10 6.4 10.6 14.6 18.6 22.1 Average 7.8 11.72 15.59 19.35 23.11

Changes in weight of pigs fed pig feed containing pig feed additive composition of Application Example 5 pig
Week 0 One 2 3 4 One 8 12.2 15.7 19.2 23.2 2 7.6 11.6 15.1 18.6 22.6 3 8.2 12.2 16.1 19.8 23.3 4 9 13 17.2 21.1 24.6 5 8.6 12.1 16.1 20 23.9 6 7.8 11.8 15.3 18.8 22.8 7 7.4 11.2 15.4 19.3 22.8 8 68 10.3 14.3 18.2 22 9 8.2 12.2 16.1 19.9 23.8 10 6.4 10.6 14.6 18.6 22.1 Average 7.8 11.72 15.59 19.35 23.11

Changes in weight of pigs fed pig feed containing pig feed additive composition of Application Example 6 pig
Week 0 One 2 3 4 One 8.0 12.1 16.1 20.2 23.9 2 8.1 11.7 15.8 19.4 23.6 3 8.1 11.8 16.0 19.4 23.7 4 8.1 11.7 15.6 19.1 22.8 5 8.0 12.1 16.3 20.1 23.8 6 8.1 12.0 16.2 20.1 24 7 8.1 12.0 15.9 19.6 23.3 8 8.0 12.2 15.9 21.4 25.8 9 8.1 12.3 15.9 19.1 23 10 8.2 12.5 16.1 20.0 23.9 Average 8.08 11.97 15.98 19.84 23.78

* In Tables 33 to 37, the weight change of pigs for pigs 1 to 10 is rounded off at the third decimal place.

Pigs ingesting pig feed (Application Example 1, Application Example 2, Application Example 5, Application Example 6) containing the feed additive composition for pigs of the present invention as shown in Table 33 to Table 37 are pigs ingesting only conventional pig feed. Compared with the higher weight gain, the growth was faster.

As described above, the present invention has been described with reference to preferred experimental examples, examples, and test examples, but a person skilled in the art should be aware of the present invention without departing from the spirit and scope of the present invention as set forth in the claims below. It will be understood that various modifications and variations can be made to the invention.

Pig feed additive composition comprising pineapple, pears, figs and rice bran in broth used as the main ingredients of pig feed of the present invention is added to the pig feed and ingested such pig feed into pigs to prevent disease, health, etc. By supplying high quality pigs to the market, it is possible to contribute to the increase of income of pig farmers and the health of consumers who eat pork.

Claims (4)

Pig feed additive composition for pigs, comprising 5 to 20 parts by weight, 5 to 20 parts by weight of pears, 5 to 20 parts by weight of figs, and 5 to 20 parts by weight of rice bran, based on 100 parts by weight of meat meal. 5 to 20 parts by weight of pineapple, 5 to 20 parts by weight of pears, 5 to 20 parts by weight of figs, and 5 to 20 parts by weight of rice bran, according to 100 parts by weight of meat, and a method for producing a feed additive composition for a pig. In pig feed,
5 to 20% by weight of the feed additive composition for pigs according to claim 1 to pig feed, or 5 to 20% by weight of the feed additive composition for pigs prepared by the method of claim 2 on pig feed Pig feed comprising a. In the production method of pig feed,
5 to 20% by weight of the feed additive composition for pigs according to claim 1 to pig feed or 5 to 20% by weight of the pig feed additive composition prepared by the method of claim 2 on pig feed Pigment feed manufacturing method characterized in that the addition and mixing.