KR101012125B1 - Label for estimating of saprogenic bacteria's growth by cumulative storage temperature' change - Google Patents

Abstract

The present invention relates to a label for determining the decay potential of a storage material according to the accumulation of storage temperature changes, specifically, a mixed powder (1) comprising a lyophilized label bacterium and a medium powder, and a mixed liquid (2) containing a pH indicator. And a mixed powder (1), distilled water (2), and the mixed powder comprising a label or lyophilized labeling bacteria, a pH labeling substance, and a medium powder comprising a pulverizing membrane 3 separating the mixed powder and the mixed liquid. It relates to a label comprising a crushing separation membrane (3) for separating the mixed solution, the label is adhesive to the outside of the food storage container, the color is changed when the label bacteria subjected to temperature changes such as the storage material is grown, Observing the color change can be useful for determining whether the storage material is corrupt.

Accumulation of temperature change, judgment of corruption, pH change

Description

Label for estimating of saprogenic bacteria's growth by cumulative storage temperature 'change}

The present invention relates to a label for determining the perishability of a storage material according to the accumulation of storage temperature changes.

Perishable substances such as foods are stored or transported refrigerated. At this time, if the storage temperature is changed or the food is exposed to the outside may cause corruption. If the food can be determined whether the storage state, such as refrigeration properly maintained until the final use after release can prevent food poisoning due to the growth of decay bacteria in advance and can help a lot in food safety management.

Methods of measuring and displaying the history of changes over time of outside air in food storage are chemically and physically possible, and some techniques have already been reported (Korean Patent 2002-0086597; US Patent 6,866,863). However, it is possible to physically measure the temperature change (time and degree, etc.) of the external environment, but to individually measure how the temperature of a substance in a storage container, for example a liquid food, has changed. It is more difficult because of various factors such as the degree of heat transfer of the vessel.

In addition, it is more difficult to determine the storage temperature and exposure time, etc. to measure and determine whether the stored food is corrupt. Specifically, it is more difficult than the measurement of the physical temperature change, it is difficult to biologically determine whether the decay bacteria ultimately grew by various types of temperature change. In other words, it is possible to predict the time and temperature required for the growth of decayed bacteria, but it is not possible to predict physically and chemically whether the decayed bacteria will actually grow due to the continuously changing temperature conditions and the different exposure times in the actual food storage space. .

Accordingly, the present inventors produce a label in a form that can be adhered to the outside of the food storage container, including the lyophilized label bacteria and medium powder, and a pH indicator and distilled water, and the label is stored in accordance with the accumulated temperature change The present invention has been completed by confirming that it can be usefully used for determining the possibility of corruption.

It is an object of the present invention to provide a label for determining the perishability of a storage material according to the accumulation of storage temperature changes.

Another object of the present invention to provide a method for determining the corruption of the storage material using the label.

In order to achieve the above object, the present invention is a mixed powder (1) comprising a lyophilized label bacteria and medium powder, a mixed liquid (2) containing a pH indicator and a separation membrane for crushing separating the mixed powder and the mixed liquid (3) Labels for determining the perishability of the storage material resulting from the accumulation of changes in storage temperature, including

In addition, the present invention is a label comprising a mixed powder (1) comprising a lyophilized label, pH labeling material and medium powder, distilled water (2) and a separation membrane (3) for separating the mixed powder and distilled water It provides a label for determining the perishability of the storage material due to the cumulative storage temperature change.

In addition, the present invention provides a method for determining the corruption of the storage material using the label.

Hereinafter, the present invention will be described in detail.

The present invention relates to a storage temperature change comprising a mixed powder (1) comprising a lyophilized label bacterium and a medium powder, a mixed liquid (2) containing a pH indicator, and a separation membrane (3) for separating the mixed powder and the mixed liquid. Provide a label for determining the perishability of the storage material as a result of

In addition, the present invention is a label comprising a mixed powder (1) comprising a lyophilized label, pH labeling material and medium powder, distilled water (2) and a separation membrane (3) for separating the mixed powder and distilled water It provides a label for determining the perishability of the storage material due to the cumulative storage temperature change.

In a specific embodiment of the present invention, the Lactobacillus acidophilus ( lactobacillus acidophilus ) as a label bacterium was cultured in various media for 0 to 21 days, and as a result, the pH was lowered due to the growth of lactic acid and the like, and was lowered once. It was confirmed that the pH was maintained up to 3 weeks (see Table 1), and the incubation of the labeled bacteria with pH indicators such as pH indicators, metal soap particles, and pH sensitive polymer particles resulted in a drop in pH due to growth of the labeled bacteria. At the same time the color of the medium was changed to indicate that it is acidic (see Table 2). At this time, in the case of metal soap particles or pH sensitive polymer particles, the acidic color was maintained even when the medium was adjusted to neutral. When the label of the present invention is cryopreserved, in order for the label to be effectively used, the bacterium is alive at a freezing temperature (for example, -20 ° C) and must be able to grow when the temperature rises. In addition, if the temperature falls below the freezing point while the label is used in the storage space, the bacteria must survive below the freezing point for the label to work properly. Above all, if stored at low temperature for a long time, the labeled bacteria should survive even though they do not grow. Thus, even if the labeled bacteria were cultured after freezing storage, it was possible to culture the same as bacteria cultured without freezing storage or cultured after cold storage, and it was confirmed that the pH of the culture solution was dropped at the same time as the culture. In addition, by lyophilizing the labeled bacteria after incubation at different inoculation concentrations, the freeze-dried labeled bacteria should use 0.1% (W / V) or less without affecting the judgment of color due to turbidity by other substances (Table 3). CFU should be 10,000 CFU / ml or more to confirm that the growth of the bacteria occurs within 1-2 days. In addition, the label prepared to include the lyophilized label and the pH indicator was exposed to various temperatures, and by comparing the color of the pH indicator according to the culture state of the microorganisms, it was possible to perform the decay determination of the storage material (See Table 4). Thus, the label of the present invention can be usefully used as a label for determining the corruption of the storage material according to the accumulation of storage temperature changes.

The bacterium has a growth pattern similar to E. coli O-157, Salmonella, etc., which is a representative decaying food poisoning bacterium. Lactic acid bacteria, yeast, fungi, etc., and most preferably Lactobacillus acidophilus ) can be used.

The medium composition was determined so that the pH of the medium is lowered by the organic acid generated at the same time as the growth of the label bacteria, preferably containing MRS, medium diluted with 25% MRS medium, minimum medium and 100 g / L of glucose Minimal medium may be used, and more preferably, a medium in which MRS medium is diluted to 25% may be used. Specifically, the pH of the medium when the labeled bacteria do not grow is neutral, and when the amount of amino acids in the medium is large, the pH is increased by ammonia generated while being degraded by the labeled bacteria. However, the marker bacteria produce organic acids in proportion to the growth rate, and these organic acids drop the pH of the medium to become acidic. In other words, when the pH is lowered, the color change indicator is added to the medium, and the change in color is caused by the growth of the labeled bacterium such as the storage material, which shows that it can grow if there are decayed bacteria in the storage material.

The mixed powder is used by mixing the lyophilized label bacteria and the medium powder in a ratio of 0.01 to 0.1: 99.9 to 99.99, preferably in a ratio of 0.05 to 0.1: 99.9 to 99.95, most preferably in a ratio of 0.1: 99.9 It is preferable.

In the preparation of the label of the present invention, in order to avoid the need to maintain a sterile condition, the label bacteria are prepared in the form of lyophilized powder, premixed with the medium powder, and separated from water. In this case, the culture of the various bacteria can be suppressed, but it is not a sterile situation, the fungus is mixed, and the mixed powder of the present invention in order to decompose the organic acid while growing the fungus when cultivating the labeled bacteria, and to increase the pH to prevent color change. A fungal growth inhibitor may be additionally added to (1), and the fungal growth inhibitor may be antifungal polyene (polyene antifungals), nystatin, amphotericin B, pimaricin And the like can be used.

The pH indicator can be used both in the neutral and acidic color change indicators, preferably methyl red, methyl blue, bromocresol Green-Methyl Red, bromocresol green (Bromocresol Green), Bremophenol Blue, etc. can be used.

However, even if the pH of the culture solution is lowered with the organic acid generated by the growth of the labeled bacteria, when the pH rises again due to growth of other contaminants, the pH may become neutral, and therefore, the color should not change even when the pH rises. That is, there is a need for a method of maintaining the color once the color is changed by the low pH. Thus, metal soap particles and pH sensitive polymer particles of the present invention can be used more usefully.

As the pH labeling material, a pH indicator, metal soap particles, and pH sensitive polymer particles may be used. The metal soap particles are formed in the form of granulated metal soap by reacting pigments and oleic acid with divalent ions such as calcium, and when the pH of the culture medium decreases due to the generation of organic acids due to the growth of the labeled bacteria, the metal soap is decomposed again. As the dye contained therein is dispersed into the medium, the label becomes the color of the pigment as a whole. This reaction allows the dye to retain its color within the label spontaneously, even if the pH changes to neutral over time as the ingredients in the label change over time (see Figure 3).

The pH-sensitive polymer particles are prepared in a form in which the pigment is collected as a basic polymer in which the polymer is expanded at a low pH. When the pH is lowered due to the generation of an organic acid, the pigment trapped in the polymer particles is released into the medium to form a pigment. The color of the pigment can be maintained because the pigment becomes colored and the pigment spread on the medium does not enter the shrinked polymer particles even when the medium is neutral again. Basic polymers include chitosan, polylysine, polyethyleneimine (PEI), diethylaminoethyl-dextran (DEAE-dextran), poly (amidoamine) dendrimers (poly (amidoamine) (PAMAM) dendrimers] and the like can be used (see FIG. 4).

In addition, D & C Red No. 6 Barium-lake, a red organic dye, an inorganic dye, and the like may be used for the pigment.

In addition, the label of the present invention can be produced in a form that can be adhered to the outside of the food storage container. It is manufactured in the form of a label that is directly attached to the outer container of the storage material that is perished by the temperature, thereby measuring the change in the actual temperature of the storage material, not the temperature of the storage space during storage and transportation.

In the embodiment of the present invention, for example, before applying the label of the present invention, the mixed powder (1) containing the lyophilized label bacteria and the medium powder and the mixed liquid (2) containing the pH indicator are separated into the separation membrane (3). It is separated. When the adhesive part is peeled off and the label is attached, the crushing separation membrane 3 is broken by the pressure applied from the outside of the label, and the mixed powder and the mixed solution are mixed so that the labeled bacteria can be cultured. At this time, the crushing separator 3 uses a curable resin so as to be easily broken, and the curable resin includes acrylic, phenol resin, urea resin, melamine resin, and the like. In addition, the outside of the label in the form of a film such as PE (poly ethylene), PP (poly propylene), PS (poly styrene), PET (Poly Ethylene Telephthalate) to protect the inner material against the pressure applied during adhesion The opaque white is used so that the end surface is not confused with the color of the storage material (see FIG. 1).

In addition,

1) breaking the separation membrane 3 for crushing while attaching the label to an outer container of the storage material;

2) After the storage period is completed, it provides a method for determining the perishability of the storage material, including the step of observing the color of the label to determine that in the case of acidic index, perishable.

In a specific embodiment of the present invention, after exposing the label of the present invention to various temperatures, by comparing the color of the pH indicator according to the culture state of the microorganisms, it was possible to perform the decay determination of the storage material (see Table 4). Thus, the method of the present invention can be usefully used to determine the perishability of the storage material due to the accumulation of storage temperature changes.

If the temperature of the storage material rises or changes due to the accumulation of changes in the external temperature during storage, the labeled bacteria in the label adhered to the outer container of the storage material will grow if the temperature is suitable for the growth of the labeled bacteria according to the temperature change. You can do it. At this time, when the pH is acidic due to the organic acid generated as the labeled bacteria grow, the possibility of corruption may be determined by checking whether the pH labeling substance has an acidic color.

The label of the present invention can be adhered to the outside of the food storage container, the color is changed when the label bacteria subjected to the temperature change, such as the storage material is changed, it is useful to determine whether the storage material decay by observing the color change Can be.

Hereinafter, the present invention will be described in detail by production examples and examples.

However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

< Production Example > Production of labels for judging the corruption of stored materials

<1> medium composition

<1-1> Minimum badge ( per liter )

K ₂ HPO ₄ 7.0 g

KH ₂ PO ₄ 2.0 g

(NH ₄ ) 2SO ₄ 1.0 g

Glucose 10.0 g

0.5 g of sodium citrate

MgSO ₄ .7H ₂ O 0.1 g

<1-2> MRS  badge( per liter )

Proteose Peptone No. 3 10.0 g

Beef Extract 10.0 g

Yeast Extract 5.0 g

Dextrose 20.0 g

Polysorbate 80 1.0 g

Ammonium Citrate 2.0 g

Sodium Acetate 5.0 g

Magnesium Sulfate 0.1 g

Manganese Sulfate 0.05 g

Dipotassium Phosphate 2.0 g

Agar 15.0 g

<2> pH  Indicator or pH  Labeling material production

<2-1> pH  Indicator

0.1 g of methyl red and 0.1 g of methylene blue are dissolved in 190 ml of ethanol and filled with water to make 200 ml. Similarly, any color change indicators that change from neutral to acidic can be applied.

<2-2> metal soap

The pigment and oleic acid were subjected to a saponification reaction with divalent ions such as calcium to prepare a metal soap grain form. After the reaction for 2.5 hours at 480 rpm at 60 ℃ with the following composition, it was used by grinding finely.

D & C Red No.6 Barium-lake 10 mg

15 g oleic acid

2.204 g of Ca (OH) ₂

H ₂ O 5.67 g

Lipase 0.0375 g

<2-3> pH  Sensitive Polymer

There are many types of pH sensitive polymers and any basic polymer that expands at low pH can be applied.

0.5 g of chitosan is dissolved in 50 ml of 0.1% (V / V) acetic acid, and EDTA can be added to 3-80% (Wt%) of chitosan to increase the strength. 1-5 mg of a pigment (D & C Red No. 6 Barium-lake; Warner-Jenkinson, USA) was added thereto, and the pH was neutralized with 5 M NaOH. EDAC [1-Ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride; Sigma, USA] was added to 10-100 mM. After stirring for 4 hours at room temperature, washed twice with distilled water, washed twice with 0.5 M NaCl, 0.025 M NaOH and twice with 0.05 M NaOH, and then washed once with distilled water to be refrigerated.

<3> label production

<3-1> pH  Including indicator

6 mg and 10 mg of lyophilized Lactobacillus esidophilus and lyophilized MRS (25%) medium were added to one side of the acrylic separated space, and 0.6 ml of distilled water containing a pH indicator was added to the other side. By putting in, a label for determining the decay potential of the storage material according to the accumulation of temperature changes was prepared (see FIG. 1).

<3-2> pH  Include labeling substance

On one side of the acrylic separated space, 6 mg, 1 mg and 10 mg, respectively, of lyophilized Lactobacillus esidophilus, pH metal soap particles or pH sensitive polymer particles, and lyophilized MRS (25%) medium were added. The other side was prepared by adding 0.6 ml of distilled water to prepare a label for judging the corruption of the storage material according to the accumulation of temperature change (see FIG. 2).

< Example  1> Marker  According to growth pH  Confirm change

Lactobacillus, Lactobacillus acidophilus ; ATCC 4356, USA) after one evening of incubation, 5% (v ) in MRS, MRS (25%) (media diluted 25% of MRS medium), minimal medium and minimal medium containing 100 g / l of glucose / v) at the rate of inoculation. While incubating at 30 ° C., OD values and pH were measured at 0, 1 day, 4 days, 7 days and 21 days.

As a result, as shown in Table 1, the Lactobacillus Etophyllus growth in MRS medium and minimal medium and the like, the pH was lowered by the production of lactic acid, etc., once lowered pH was maintained even up to 3 weeks.

At this time, since the medium color of the MRS 100% medium is difficult to observe the color change of the pH indicator, it was easy to observe the color change by diluting the MRS medium to 25% to make the medium color lighter.

Medium / incubation time Immediately after inoculation 1 day 4 days 7 days 21st OD pH OD pH OD pH OD pH OD pH MRS 0.05 6.7 8.6 3.6 6.8 3.7 6.3 3.9 6.8 3.7 MRS (25%) 0.01 6.7 3.7 3.6 2.7 3.6 2.6 3.8 2.7 3.8 Minimum badge 0 7.2 0.3 6.4 0.5 4.4 0.4 4.4 0.5 4.5 Minimum medium + glucose (100 g / L) 0 7 0.4 4.7 0.4 4.8 0.3 4.7 0.3 4.5

< Example  2> Marker  According to growth pH  Change and pH  Check the color change of the label

Lactobacillus Escidophilus, a lactic acid bacterium, was inoculated in MRS (25%) medium containing pH indicator, metal soap particles, and pH sensitive polymer particles at concentrations of 0.0001%, 1%, and 1%, respectively, and cultured at 30 ° C. At 4 days, 1 week, 3 weeks, and 6 weeks, pH changes and color changes of pH markers were confirmed. Finally, the pH was corrected to neutral and the color change was confirmed.

As a result, as shown in Table 2, the pH indicator, which was initially green, turned purple and red to maintain color for up to 6 weeks, and in the case of metal soap particles or pH-sensitive polymer particles, the color was maintained even when the medium was kept neutral. Red color remained.

Immediately after inoculation 4 days 1 week 3 weeks 6 weeks pH 6.7 3.8 3.6 3.8 3.8 pH indicator green often often often often Metal soap particles Colorless Red Red Red Red pH sensitive polymer particles Colorless Red Red Red Red

< Example  3> frozen storage Marker  Survival test

After inoculating Lactobacillus Escidophilus, which is a lactic acid bacterium, into the MRS (25%) medium and incubating at 30 ° C. for 24 hours, the culture solution was stored at −10 ° C. and −20 ° C. for 48 hours. After culturing the culture solution stored at low temperature for 24 hours at 30 ℃, the OD value was measured.

As a result, the OD values of the control group (cultivated at 30 ° C. without storage), the -10 ° C. experimental group, and the -20 ° C. experimental group were 3.8, 3.6, and 3.5, respectively, indicating that the labeled bacteria that had been stored frozen can also be cultured.

< Example  4> Lyophilized Powder Marker and  Cultured for 1 day Inoculation  Comparison of effects when using

Lactobacillus Escidophilus, a lactic acid bacterium, was inoculated in MRS (25%) medium and incubated at 30 ° C. for 24 hours, followed by centrifugation of the culture bacteria and suspended in a nutrient (10% Skim milk + 1% sodium glutamin solution). It was then lyophilized using Eyela lyophilizer (Japan). The lyophilized powder labeled bacteria and the culture medium of the liquid cultured by the above method were inoculated at a concentration of 10% in MRS (25%) medium and incubated at 30 ° C. for 24 hours.

As a result, less than 0.1% (W / V) of the freeze-dried marker bacteria should not affect the judgment of color due to turbidity by other substances, and CFU should be 10,000 CFU / ml or higher. It takes place within two days.

Powder bacteria inoculation rate (w / v) 1 day 3 days 10% 4.80 3.37 5% 5.20 4.71 One% 4.98 3.38 0.5% 4.07 4.43 0.1% 4.15 3.40 0.01% 4.7 3.82

< Example  5> Color change by storage temperature

After the label prepared in Preparation Example 3 was exposed to various temperatures such as -10 ° C, 4 ° C, 15 ° C, and 37 ° C for a predetermined time, as defined in Table 4, each section (0-6, 6-20, 20). Color changes in ˜28, 28-42 and 42-48) were observed.

As a result, a color change appeared as shown in Table 4, and the possibility of corruption could be determined based on the color change.

Experiment 0-6 hours 6-20 20-28 28-42 42-48 Corruption One Temperature 37 37 37 37 37 Corruption
possible Color green Purple Purple Purple Purple 2 Temperature 15 15 15 15 15 Corruption
possible Color green green green Green purple Purple 3 Temperature 4 4 4 4 4 safety Color green green green green green 4 Temperature 4 15 4 4 4 safety Color green green green green green 5 Temperature 4 4 15 4 4 safety Color green green green green green 6 Temperature 4 37 37 4 4 Corruption
possible Color green green Purple Purple Purple 7 Temperature -10 37 37 -10 -10 Corruption
possible Color green green Purple Purple Purple 8 Temperature -10 37 4 37 37 Corruption
possible Color green green green Purple Purple

1 is a block diagram of an adhesive label containing a pH indicator of the present invention.

Figure 2 is a block diagram of an adhesive label containing a pH labeling material of the present invention.

3 is a view showing the color development principle of metal soap particles.

Figure 4 is a diagram showing the color development principle of the pH sensitive polymer.

Claims (26)

Accumulation of storage temperature changes includes a mixed powder (1) comprising a lyophilized label bacterium and a medium powder, a mixed liquid (2) containing a pH indicator, and a crushing separator (3) separating the mixed powder and the mixed liquid. Label for judging the corruption of the storage material according to Accumulation of storage temperature changes including a mixed powder (1) comprising a lyophilized label bacterium, a pH labeling substance and a medium powder, distilled water (2) and a separation membrane (3) separating the mixed powder and distilled water Label for judging the corruption of the stored material. [Claim 3] The storage temperature change of claim 1 or 2, wherein the labeled bacterium is a microorganism having a growth pattern similar to that of the decaying food poisoning bacterium, which forms an organic acid upon growth, lowers the pH, and is harmless to humans and the environment. Label for judging the corruption of the stored material by accumulation of According to claim 3, wherein the decayed food poisoning bacteria E. coli O-157 or Salmonel line characterized in that the storage material according to the accumulation of storage temperature changes, the possibility of decay label. According to claim 3, wherein the label is a label for judging the corruption of the storage material according to the accumulation of storage temperature changes, characterized in that selected from the group consisting of lactic acid bacteria, yeast, mold. The method of claim 5, wherein the label is Lactobacillus Edophilus ( Lactobacillus acidophilus ) characterized in that the label for determining the corruption of the storage material according to the accumulation of storage temperature changes. The method of claim 1 or 2, wherein the medium is a composition in which the pH of the medium is lowered by the organic acid generated at the same time as the growth of the label bacteria, the determination of the corruption of the storage material due to the accumulation of storage temperature changes label. 8. The storage temperature change of claim 7, wherein the medium is any one selected from the group consisting of MRS, medium diluted with 25% MRS medium, a minimum medium and a minimum medium containing 100 g / l of glucose. Label for judging the corruption of the stored material by accumulation of The method of claim 8, wherein the medium is a medium in which MRS medium is diluted 25%. The method of claim 1 or 2, wherein the mixed powder is used by mixing the lyophilized label bacteria and the medium powder in a ratio of 0.01 ~ 0.1: 99.99 ~ 99.9 of the storage material according to the cumulative storage temperature change Perishable label. 11. The method of claim 10, wherein the mixed powder is used to mix the lyophilized label bacteria and the medium powder at a ratio of 0.05 ~ 0.1: 99.95 ~ 99.9 to determine the possibility of corruption of the storage material according to the accumulation of storage temperature changes Dragon label. The label of claim 11, wherein the mixed powder is used by mixing the lyophilized label bacteria and the medium powder in a ratio of 0.1: 99.9. The label of claim 1 or 2, wherein the mixed powder further includes a fungus growth inhibitor. The method of claim 13, wherein the fungal growth inhibitor is selected from the group consisting of antifungal polyene (polyene antifungals), nystatin, amphotericin B (amphotericin B), pimaricin (pimaricin) Label for judging the decay of storage materials with accumulation of storage temperature changes. According to claim 1, wherein the pH indicator is a label for judging the corruption of the storage material according to the cumulative storage temperature change, characterized in that the change in color is distinct from neutral and acidic. 16. The label of claim 15, wherein the pH indicator is a mixture of methyl red and methyl blue. The label for determining the corruption of the storage material according to the accumulation of storage temperature changes, characterized in that the pH labeling material is metal soap particles or pH sensitive polymer particles. 18. The label of claim 17, wherein the metal soap particles are formed in the form of metal soap particles by saponifying a pigment and oleic acid with divalent ions. delete 18. The method of claim 17, wherein the pH sensitive polymer is chitosan, polylysine, polyethyleneimine (PEI), diethylaminoethyl-dextran (DEAE-dextran) and poly (amidoamine). Label for determining the corruption of the storage material according to the accumulation of storage temperature changes, characterized in that selected from the group consisting of dendrimers (poly (amidoamine) (PAMAM) dendrimers). The label of claim 1 or 2, wherein the label is made in a form that can be adhered to the outside of the food storage container. The label of claim 1 or 2, wherein the shredding separator (3) is a curable resin. 23. The label of claim 22, wherein the curable resin is selected from the group consisting of acrylic, phenolic resins, urea resins and melamine resins. The method according to claim 1 or 2, wherein the label outside is selected from the group consisting of polyethylene (PE), poly (propylene), polystyrene (PS) and poly ethylene telephthalate (PET). Label for judging the decay of storage materials with accumulation of storage temperature changes. The label of claim 1 or 2, wherein the adhesive bottom surface of the label is opaque white. 1) breaking the crushing separator 3 while attaching the label of claim 1 to the outer container of the storage material; 2) after the storage period is completed, if the acid index by observing the color of the label, the method of determining the corruption of the storage material comprising the step of determining that the corruption is possible.

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