WO2015190772A1 - Functional label, having lactobacillus coating for detecting external impact, for checking freshness of storage material - Google Patents

Functional label, having lactobacillus coating for detecting external impact, for checking freshness of storage material Download PDF

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Publication number
WO2015190772A1
WO2015190772A1 PCT/KR2015/005702 KR2015005702W WO2015190772A1 WO 2015190772 A1 WO2015190772 A1 WO 2015190772A1 KR 2015005702 W KR2015005702 W KR 2015005702W WO 2015190772 A1 WO2015190772 A1 WO 2015190772A1
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WO
WIPO (PCT)
Prior art keywords
layer
coating
lactic acid
indicator
acid bacteria
Prior art date
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PCT/KR2015/005702
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French (fr)
Korean (ko)
Inventor
한윤석
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한윤석
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Publication date
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Publication of WO2015190772A1 publication Critical patent/WO2015190772A1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02—Food
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
    • G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
    • G01N31/221—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators for investigating pH value
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
    • G01K11/00—Measuring temperature based upon physical or chemical changes not covered by groups G01K3/00, G01K5/00, G01K7/00 or G01K9/00
    • G01K11/12—Measuring temperature based upon physical or chemical changes not covered by groups G01K3/00, G01K5/00, G01K7/00 or G01K9/00 using changes in colour, translucency or reflectance
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01L—MEASURING FORCE, STRESS, TORQUE, WORK, MECHANICAL POWER, MECHANICAL EFFICIENCY, OR FLUID PRESSURE
    • G01L5/00—Apparatus for, or methods of, measuring force, work, mechanical power, or torque, specially adapted for specific purposes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • G01N21/80—Indicating pH value
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7756—Sensor type
    • G01N2021/7759—Dipstick; Test strip

Definitions

  • the present invention relates to a functional label for external shock detection lactic acid bacteria coating for checking the freshness of a storage material, and more particularly, a pH indicator layer and a lactic acid bacterium coating core layer are attached to an upper portion of the label, so that the coating layer is broken by an external impact.
  • the present invention relates to a lactic acid bacteria coating functional label for external shock detection for freshness check of storage materials that can be checked for freshness.
  • Perishable substances such as foods are stored or transported refrigerated. At this time, if the storage temperature is changed or the food is exposed to the outside may cause corruption. If it is possible to determine whether the food has been properly stored in the refrigerator until its final use after release, it can prevent food poisoning due to the growth of decayed bacteria and can be helpful for food safety management.
  • the method of measuring and displaying the history of the change of the external air with time during food storage is chemically and physically possible, and it is possible to physically measure the time and degree as the temperature change of the external environment, but the liquid food as the substance in the storage container.
  • the individual measurement of how the temperature of the furnace has changed is more difficult due to various factors such as the condition of the storage space and the degree of heat transfer in the container.
  • a label may be prepared in a form that can be adhered to the outside of the food storage container including the lyophilized lactic acid bacteria and the medium powder and the pH indicator and the distilled water, and the label may be used to determine the perishability of the storage material due to the accumulation of temperature change.
  • the present invention relates to a pH indicator layer and a lactic acid bacteria coating core layer attached to the upper part of the label, and to an external shock detection lactic acid bacterium coating functional label for checking the freshness of the storage material when the coating layer is broken by an external impact. will be.
  • the functional label of the external shock detection lactic acid bacterium coating for checking the freshness of the storage material of the present invention can be adhered to the outside of the food storage container, and the color changes when the lactic acid bacteria are grown under the same temperature change as the storage material. You can judge the freshness.
  • 1 to 22 are exploded cross-sectional view showing a functional label for checking the freshness of the storage material of the present invention.
  • the present invention includes a pH indicator layer 24 attached to an upper surface of the label 10 and a lactic acid bacteria coating core layer 38 attached to an upper portion of the pH indicator layer 24;
  • a lactic acid bacteria coating core layer 38 attached to the upper surface of the label 10 and a pH indicator layer 24 attached to the upper portion of the lactic acid bacteria coating core layer 38; It consists of any two layers selected from,
  • the pH indicator layer 24 is composed of any one indicator selected from the pH indicator powder 20 and the pH indicator liquid 22,
  • the lactic acid bacteria coating core layer 38 is a lactic acid bacteria liquid 32 is embedded in the lactic acid bacteria coating core 36, the outer layer of the lactic acid bacteria liquid layer as a coating layer 50 for the external shock detection that the coating layer is broken by an external impact.
  • the present invention relates to a label for labeling a lactic acid bacteria functional label for external shock detection of freshness of a storage material, which comprises a plurality of coated lactic acid bacteria coating cores 36.
  • the outer shock detection lactic acid bacteria functional label is characterized in that the surface coating layer 40 is further coated on the two layers.
  • the present invention includes a pH indicator layer 24 attached to an upper surface of the label 10 and a lactic acid bacteria coating core layer 38 attached to an upper portion of the pH indicator layer 24; And a pH indicator layer 24 attached to an upper portion of the lactic acid bacteria coating core layer 38 and the lactic acid bacteria coating core layer 38 attached to the upper surface of the label 10. It consists of any two layers selected from,
  • the pH indicator layer 24 is composed of a pH indicator liquid 22,
  • the lactic acid bacteria coating core layer 38 is a lactic acid bacteria powder 30 is embedded in the lactic acid bacteria coating core 36, the coating layer for the external impact detection layer 50 is broken by an external impact on the outside of the lactic acid bacteria powder layer.
  • the present invention relates to a label for labeling a lactic acid bacteria functional label for external shock detection of freshness of a storage material, which comprises a plurality of coated lactic acid bacteria coating cores 36.
  • the present invention includes a pH indicator coating core layer 28 attached to an upper surface of the label 10 and a lactic acid bacteria layer 34 attached to an upper portion of the pH indicator coating core layer 28; And a pH indicator coating core layer 28 attached to an upper portion of the lactic acid bacteria layer 34 and the lactic acid bacteria coating core layer 38 attached to the upper surface of the label 10. It consists of any two layers selected from,
  • the lactic acid bacteria layer 34 is composed of any one lactic acid bacteria selected from lactic acid bacteria powder 30 and the liquid lactic acid bacteria 32,
  • the pH indicator coating core layer 28 is a pH indicator liquid 22 is embedded in the pH indicator coating core 26, the pH indicator liquid as the coating layer 50 for the external shock detection that the coating layer is broken by an external impact.
  • the present invention relates to an external shock detection lactic acid bacterium coating functional label for checking the freshness of a storage material, comprising a plurality of pH indicator coating cores 26 coated on the outside of the layer.
  • the label of the present invention is characterized in that the surface coating layer 40 is further coated on top of the two layers of the pH indicator coating core layer 28 and the lactic acid bacteria layer 34.
  • the present invention includes a pH indicator coating core layer 28 attached to the upper surface of the label 10 and a lactic acid bacteria layer 34 attached to the top of the pH indicator coating core layer 28; And a pH indicator coating core layer 28 attached to an upper portion of the lactic acid bacteria layer 34 and the lactic acid bacteria coating core layer 38 attached to the upper surface of the label 10. It consists of any two layers selected from,
  • the lactic acid bacteria layer 34 is made of lactic acid bacteria powder 30,
  • the pH indicator coating core layer 28 is a pH indicator liquid 22 is embedded in the pH indicator coating core 26, the pH indicator liquid as the coating layer 50 for the external shock detection that the coating layer is broken by an external impact.
  • the present invention relates to an external shock detection lactic acid bacterium coating functional label for checking the freshness of a storage material, comprising a plurality of pH indicator coating cores 26 coated on the outside of the layer.
  • the label of the present invention is characterized in that the surface coating layer 40 is further coated on top of the two layers of the pH indicator coating core layer 28 and the lactic acid bacteria layer 34.
  • the present invention shows a pH indicator coating core layer 28 attached to the upper surface of the label 10 and a lactic acid bacteria coating core layer 38 attached to the top of the pH indicator coating core layer 28. ); And a pH indicator coating core layer 28 attached to the top of the lactic acid bacteria coating core layer 38 and the lactic acid bacteria coating core layer 38 attached to the upper surface of the label 10. It consists of any two layers selected from,
  • the pH indicator coating core layer 28 has any one indicator selected from the pH indicator powder 20 and the pH indicator liquid 22 inside the pH indicator coating core 26, and the coating layer is broken by an external impact.
  • the external shock detection coating layer 50 is composed of a plurality of pH indicator coating core 26 coated on the outside of the pH indicator layer,
  • the lactic acid bacteria coating core layer 38 is a lactic acid bacteria liquid 32 is embedded in the lactic acid bacteria coating core 36, the outer layer of the lactic acid bacteria liquid layer as a coating layer 50 for the external shock detection that the coating layer is broken by an external impact.
  • the present invention relates to a label for labeling a lactic acid bacteria functional label for external shock detection of freshness of a storage material, which comprises a plurality of coated lactic acid bacteria coating cores 36.
  • the label of the present invention is characterized in that the surface coating layer 40 is further coated on top of two layers of the pH indicator coating core layer 28 and the lactic acid bacteria coating core layer 38.
  • the present invention shows a pH indicator coating core layer 28 attached to the upper surface of the label 10 and a lactic acid bacteria coating core layer 38 attached to the top of the pH indicator coating core layer 28. );
  • the pH indicator coating core layer 28 has a pH indicator liquid 22 embedded in the pH indicator coating core 26, the coating layer for external impact detection that the coating layer is broken by an external impact on the outside of the pH indicator layer ( 50) a plurality of pH indicator coating core (26) coated with
  • the lactobacillus coating core layer 38 is a lactic acid bacteria powder 32 is embedded in the lactic acid bacteria coating core 36, the outer layer of the lactic acid bacteria powder layer as an external impact detection coating layer 50 is broken by an external impact.
  • the present invention relates to a label for labeling a lactic acid bacteria functional label for external shock detection of freshness of a storage material, which comprises a plurality of coated lactic acid bacteria coating cores 36.
  • the label of the present invention is characterized in that the surface coating layer 40 is further coated on top of two layers of the pH indicator coating core layer 28 and the lactic acid bacteria coating core layer 38.
  • a mixed layer of the pH indicator coating core layer 28 and the lactic acid bacteria coating core layer 38 is attached to the upper surface of the label 10,
  • the pH indicator coating core layer 28 has any one indicator selected from the pH indicator powder 20 and the pH indicator liquid 22 inside the pH indicator coating core 26, and the coating layer is broken by an external impact. It consists of a plurality of pH indicator coating core (26) coated on the outside of the pH indicator layer to the external impact detection coating layer (50),
  • the lactic acid bacteria coating core layer 38 is a lactic acid bacteria liquid 32 is embedded in the lactic acid bacteria coating core 36, the outer layer of the lactic acid bacteria liquid layer as a coating layer 50 for the external shock detection that the coating layer is broken by an external impact.
  • the present invention relates to a label for labeling a lactic acid bacteria functional label for external shock detection of freshness of a storage material, which comprises a plurality of coated lactic acid bacteria coating cores 36.
  • the label of the present invention is characterized in that the surface coating layer 40 is further coated on top of the mixed layer.
  • the present invention is attached to the mixed layer of the pH indicator coating core 26 and lactic acid bacteria coating core 36 on the upper surface of the label 10,
  • the pH indicator coating core 26 has any one indicator selected from the pH indicator powder 20 and the pH indicator liquid 22 inside the pH indicator coating core 26, the outer coating layer is broken by an external impact. It is coated on the outside of the pH indicator layer with a shock-sensitive coating layer 50,
  • the lactic acid bacteria coating core 36 is a lactic acid bacteria liquid 32 is embedded in the lactic acid bacteria coating core 36, the coating layer 50 for the external shock detection that the coating layer is broken by an external impact is coated on the outside of the lactic acid bacteria liquid layer. It relates to a functional label for external shock detection lactic acid bacteria coating for checking the freshness of the storage material, characterized in that it is.
  • the label of the present invention is characterized in that the surface coating layer 40 is further coated on top of the mixed layer.
  • the present invention is attached to the mixed layer of the pH indicator coating core 26 and lactic acid bacteria coating core 36 on the upper surface of the label 10,
  • the pH indicator coating core 26 is a pH indicator liquid 22 is built in the pH indicator coating core 26, the coating layer for the external shock detection coating layer 50 is broken by an external impact of the pH indicator layer of Coated on the outside,
  • the lactic acid bacteria coating core 36 is a lactic acid bacterium coating core 36 is embedded in the lactic acid bacteria powder 30, the coating layer 50 for the external shock detection that the coating layer is broken by an external impact is coated on the outside of the lactic acid bacteria liquid layer. It relates to a functional label for external shock detection lactic acid bacteria coating for checking the freshness of the storage material, characterized in that it is.
  • the label of the present invention is characterized in that the surface coating layer 40 is further coated on top of the mixed layer.
  • 11 to 12 is a mixed layer of the pH indicator powder 20 and the lactic acid bacteria coating core 36 is attached to the upper surface of the label 10,
  • the lactic acid bacteria coating core 36 is a lactic acid bacteria liquid 32 is embedded in the lactic acid bacteria coating core 36, the coating layer 50 for the external shock detection that the coating layer is broken by an external impact is coated on the outside of the lactic acid bacteria liquid layer. It relates to a functional label for external shock detection lactic acid bacteria coating for checking the freshness of the storage material, characterized in that it is.
  • the label of the present invention is characterized in that the surface coating layer 40 is further coated on top of the mixed layer.
  • the present invention is attached to the mixed layer of the pH indicator coating core 26 and lactic acid bacteria powder 30 on the upper surface of the label (10),
  • the pH indicator coating core 26 is a pH indicator liquid 22 is embedded in the pH indicator coating core 26, the coating layer for the external shock detection coating 50 is broken by an external impact of the pH indicator layer of
  • the present invention relates to an external shock detection lactic acid bacterium coating functional label for checking the freshness of a storage material, which is coated on the outside.
  • the label of the present invention is characterized in that the surface coating layer 40 is further coated on top of the mixed layer.
  • the present invention relates to a label for functional label for external shock detection lactic acid bacteria that can check the freshness of the storage material when the outside temperature rises to -10 to 40 °C in a frozen food such as frozen dumplings frozen.
  • the coating layer 50 is a soft resin for detecting the external impact that the coating layer 50 is broken when the coating layer formed in a frozen state or a low temperature state is applied with a label of about 1 to 500 psi from the outside after the label is applied to the storage material. Coating layer.
  • the coating layer 50 includes all of the natural soft resin coating layer and the synthetic soft resin coating layer in which the coating layer 50 is broken when an impact of about 1 to 500 psi is applied from the outside.
  • the synthetic soft resin coating layer is made of PE (poly ethylene), PP (poly propylene), PS (Poly styrene), PET (Poly Ethylene Telephthalate), poly epoxy resin, acrylic resin, phenol resin, urea resin, melamine resin It is preferably selected from the group.
  • the coating layer 50 for the external impact detection which is broken by the external impact, includes acrylic, phenol resin, urea resin, melamine resin, and the like.
  • the coating layer 50 formed in the frozen state or the low temperature state is subjected to an impact of about 1 to 500 psi from the outside, the coating layer 50 is broken and the mixed powder and the mixed liquid are mixed so that the lactic acid bacteria can be cultured.
  • Curable resins include acrylic, phenol resins, urea resins and melamine resins.
  • the label is preferably produced in a form that can be adhered to the outside of the food storage container
  • the external impact detection coating layer 50 is preferably a curable resin
  • the curable resin is acrylic, phenol resin, urea resin and It is preferably selected from the group consisting of melamine resins.
  • the lactic acid bacteria coating core 36 is preferably coated with lactic acid bacteria powder 30 in a frozen state, a low temperature state or a room temperature state with the coating layer 50.
  • the lactic acid bacteria coating core 36 is preferably coated with the lactic acid bacteria liquid 32 in a frozen state, a low temperature state or a normal temperature state with the coating layer 50.
  • the pH indicator coating core 26 is preferably coated with the pH indicator powder 20 in the freezing state, low temperature or room temperature state with the coating layer 50.
  • the pH indicator coating core 26 is preferably coated with the pH indicator liquid 22 in the freezing state, low temperature or room temperature state with the coating layer 50.
  • the label 10 of the present invention is separated into a coating layer 50 for external shock detection, in which a mixed powder containing a lyophilized lactic acid bacterium and a medium powder and a mixed solution including a pH indicator are broken by an external impact.
  • the label 10 of the present invention peels off an adhesive portion such as a double-sided tape, and when the external temperature rises to -10 to 40 ° C. in a state in which the label 10 is attached, the coating layer 50 for external impact detection that the coating layer is broken by an external impact. ) Is a functional label for detecting broken temperature changes.
  • the label 10 of the present invention when the coating layer 50 formed in a frozen state or a low temperature state is applied with an impact of about 1 to 500 psi from the outside, the coating layer 50 is broken and the mixed powder and the mixed solution are mixed so that lactic acid bacteria can be cultured. It becomes the state that I can.
  • the present invention provides a method for determining the decay potential of the storage material to determine that the coating layer 50 is broken when the external impact is applied after the label is attached to the outer container of the storage material, and that the label is decomposable when the acid index is observed. do.
  • the label of the present invention can perform the decay determination of the storage material by comparing the color of the pH indicator according to the fracture of the coating layer 50 after exposure to various temperatures.
  • the method of the present invention can be usefully used to determine the perishability of the storage material due to the accumulation of storage temperature changes.
  • the temperature of the storage material rises or changes due to the accumulation of changes in the external temperature during storage, the lactic acid bacteria and the pH indicator in the label adhered to the outer container of the storage material are mixed.
  • the pH becomes acidic by the lactic acid bacteria in the present invention it is possible to determine the possibility of corruption by checking whether the pH labeling substance has an acidic color.
  • the coating layer 50 is a soft resin coating layer for shock change detection broken when an impact is applied.
  • the coating layer 50 includes all of the natural soft resin coating layer and the synthetic soft resin coating layer.
  • the synthetic soft resin coating layer is preferably a coating layer made of sugar.
  • the component that can be prepared in the coating layer 50 in the present invention is preferably a food additive sugar composition.
  • the coating layer 50 is preferably used to select from maltose, glucose, fructose, maltose and sugar.
  • monosaccharides are classified into bisaccharides, trisaccharides, tetrasaccharides, pentoses, and hexasaccharides, depending on the number of carbons.
  • an important monosaccharide is hexose.
  • Glucose is the central source of sugar metabolism in the body and is the most basic source of energy in the body. It is found in vegetables and fruits, and especially in the juice of grapes.
  • Fructose is present in fruits and honey, the sweetest of sugars, and the building block of sugar and invert sugar.
  • Disaccharides include sugar (sucrose), maltose (lactose) and lactose (lactose).
  • Sugar sucrose
  • lactose lactose
  • Lactose lactose
  • Lactose lactose
  • Oligosaccharides are polysaccharides, constituents of glycoproteins or glycolipids, mainly attached to biological membranes within cells, bound to secretory proteins such as endoplasmic reticulum and Golgi, and include galactooligosaccharides, isomaltoligosaccharides, and fructooligosaccharides.
  • Polysaccharides, called complex carbohydrates, are divided into starch, glycogen, and dietary fiber.
  • Hexoxan is starch and starch.
  • Glycogen is an animal carbohydrate that is stored in muscle tissue and liver.
  • Cellulose is a polysaccharide.
  • the coating layer 50 used in the present invention is heated to flaxseed at 80 ⁇ 120 °C for 10 to 40 minutes to remove moisture from the flaxseed and then rapidly cooled, put the fast cooled flaxseed in the milking machine temperature 80 ⁇ 120 °C Malodor produced by filtering flaxseed oil by milking in a state of 200 mesh to obtain a filtered flaxseed oil, and then purifying flaxseed oil by natural sedimentation of the filtered flaxseed oil for 10 to 20 days to obtain an upper layer. It is preferable to use flaxseed oil which is free of cyanide (-CN) toxicity.
  • -CN cyanide
  • the coating layer 50 component used in the present invention is preferably added to 5 to 10% by weight of any antioxidant selected from green tea extract, ⁇ -tocophenol and rosemary.
  • the coating layer 50 component used in the present invention preferably adds 1 to 5% by weight of a phase change induction catalyst, and the phase transition catalyst includes rapeseed oil, canola oil; At least one edible emulsifier selected from the group consisting of soy protein restin, egg restin, tween, monogreen, polyglycerin and ester fatty acids; And one or more selected from the group consisting of glyceryl monooleate, glyceryl monolinoleate, glyceryl monoarachidonate and glyceryl monostearate. Monoglycerides; It is preferable that it is any one selected from among.
  • the coating layer 50 of the present invention it is preferable to use a sugar, starch, a phase change coating agent and a phase change induction catalyst to mix phase change while rotating at high speed at 3000 rpm.
  • the coating layer 50 of the present invention may be prepared by mixing an appropriate amount of sugar, starch, phase change coating agent and phase transition induction catalyst to control the composition and content of the mixture and to adjust the thickness of the coating layer to produce a coating layer that is broken by external impact.
  • the linseed was heated at 100 ° C. for 20 minutes to remove moisture from the flaxseed and then rapidly cooled.
  • the fast-cooled flaxseed was placed in an milking machine and milked at a milking machine temperature of 100 ° C. and filtered at 200 mesh to obtain filtered linseed oil.
  • -CN odor and cyanide
  • phase change water-soluble linseed oil composition 50 g of flaxseed oil prepared in Example 1 and 20 g of tocophenol were mixed in 25 g of water, and 5 g of glyceryl monooleate was added thereto, followed by coating at high speed at 3000 rpm to prepare a phase change water-soluble linseed oil composition as a phase change coating agent.
  • the coating layer consisting of 50% by weight of the malt, 45% by weight of the phase change coating agent, and 5% by weight of the phase induction catalyst is selectively dissolved at -10 ° C, and 50% by weight, starch 5% by weight, 40% by weight of the phase change coating agent, and phase induction catalyst
  • the coating layer consisting of 5% by weight is selectively dissolved at 0 ° C
  • the coating layer consisting of 50% by weight of starch, 10% by weight of starch, 35% by weight of phase change coating agent, and 5% by weight of phase induction catalyst is selectively dissolved at 5 ° C, and 50% by weight
  • the coating layer consisting of 20% by weight, 20% by weight starch, 25% by weight phase transition coating, and 5% by weight phase induction catalyst is selectively dissolved at 10 ° C., 40% by weight, 20% by weight starch, 35% by weight phase change coating, and phase transition.
  • the coating layer consisting of 5% by weight of the induction catalyst is selectively dissolved at 15 ° C, and the coating layer consisting of 50% by weight of starch, 20% by weight of starch, 25% by weight of phase change coating agent, and 5% by weight of phase induction catalyst is selectively dissolved at 20 ° C.
  • phase induction catalyst 50 wt% per starch, 25 wt% starch, 20 wt% phase change coating agent, and 5 wt% phase transition induction catalyst were selectively dissolved at 25 ° C., 50 wt% per starch, 30 wt% starch, phase change coating 25
  • the coating layer consisting of the weight percent, and 5% by weight of the phase induction catalyst is selectively dissolved at 30 ° C, and the coating layer of 40% by weight, starch 40% by weight, 15% by weight of the phase change coating agent, and 5% by weight of the phase induction catalyst is 35 ° C.
  • Is selectively dissolved at 30% by weight, 50% by weight starch, 15% by weight phase change coating agent, and 5% by weight phase induction catalyst are selectively dissolved at 40 ° C.
  • the coating layer is broken by external impact.
  • the coating layer consisting of 50% by weight glucose, 45% by weight phase change coating agent, and 5% by weight phase induction catalyst was selectively dissolved at ⁇ 10 ° C., 50% by weight glucose, 5% by weight starch, 40% by weight phase change coating agent, and phase induction catalyst.
  • the coating layer consisting of 5% by weight is selectively dissolved at 0 ° C, and the coating layer consisting of 50% by weight of glucose, 10% by weight of starch, 35% by weight of phase change coating agent, and 5% by weight of phase induction catalyst is selectively dissolved at 5 ° C.
  • the coating layer consisting of 20% by weight, 20% by weight starch, 25% by weight phase transition coating, and 5% by weight phase induction catalyst is selectively dissolved at 10 ° C., 40% by weight glucose, 20% by weight starch, 35% by weight phase change coating, and phase transition.
  • the coating layer consisting of 5% by weight of the induction catalyst is selectively dissolved at 15 ° C, and the coating layer consisting of 50% by weight of glucose, 20% by weight of starch, 25% by weight of phase change coating agent, and 5% by weight of phase induction catalyst is 20 ° C.
  • the coating layer consisting of 50% by weight of glucose, 25% by weight starch, 20% by weight phase change coating agent, and 5% by weight phase transition induction catalyst is selectively dissolved at 25 °C, 50% by weight of glucose, 30% by weight starch, phase transition
  • the coating layer consisting of 25% by weight of the coating agent, and 5% by weight of the phase induction catalyst is selectively dissolved at 30 ° C.
  • the coating layer of 40% by weight of glucose, 40% by weight of starch, 15% by weight of the phase change coating agent, and 5% by weight of the phase induction catalyst is The coating layer is selectively dissolved at 35 ° C.
  • the coating layer consisting of 30% by weight of glucose, 50% by weight of starch, 15% by weight of phase change coating agent, and 5% by weight of phase induction catalyst is selectively dissolved at 40 ° C.
  • the coating layer is broken by external impact.
  • the coating layer consisting of 50% by weight of malt sugar, 45% by weight of phase transition coating, and 5% by weight of phase induction catalyst is selectively dissolved at -10 ° C, and 50% by weight of malt, 5% by weight of starch, 40% by weight of phase change coating, and phase induction catalyst.
  • the coating layer consisting of 5% by weight is selectively dissolved at 0 ° C, and the coating layer consisting of 50% by weight of malt sugar, 10% by weight of starch, 35% by weight of phase change coating agent, and 5% by weight of phase induction catalyst is selectively dissolved at 5 ° C and 50% by weight of malt.
  • the coating layer consisting of 20% by weight, 20% by weight starch, 25% by weight phase transition coating, and 5% by weight phase induction catalyst is selectively dissolved at 10 ° C., 40% by weight, 20% by weight starch, 35% by weight phase change coating, and phase transition.
  • the coating layer consisting of 5% by weight of the induction catalyst is selectively dissolved at 15 ° C, and the coating layer consisting of 50% by weight of malt sugar, 20% by weight of starch, 25% by weight of phase change coating agent, and 5% by weight of phase induction catalyst is 20 ° C. Is selectively dissolved at 50 ° C., malt 50% by weight, starch 25% by weight, phase change coating 20% by weight, and phase change induction catalyst 5% by weight.
  • the coating layer consisting of 25% by weight of the coating agent, and 5% by weight of the phase induction catalyst is selectively dissolved at 30 ° C.
  • the coating layer of 40% by weight of malt, starch 40% by weight, 15% by weight of the phase change coating agent, and 5% by weight of the phase induction catalyst is The coating layer is selectively dissolved at 35 ° C.
  • the coating layer consisting of 30% by weight of malt sugar, 50% by weight starch, 15% by weight phase transfer coating agent, and 5% by weight phase induction catalyst is selectively dissolved at 40 ° C.
  • the coating layer is broken by external impact.
  • the coating layer composed of 50% by weight malt sugar, 45% by weight phase change coating agent, and 5% by weight phase induction catalyst prepared in Example 8 was not broken at -10 ° C for 0 to 48 hours.
  • the coating layer composed of 50% by weight of malt sugar, 5% by weight starch, 40% by weight phase change coating agent, and 5% by weight phase induction catalyst prepared in Example 8 did not break at 0 ° C for 0 to 48 hours.
  • the coating layer composed of 50% by weight malt, 10% by weight starch, 35% by weight phase change coating agent, and 5% by weight phase induction catalyst prepared in Example 8 was not broken at 5 ° C. for 0 to 48 hours.
  • the coating layer made of 50% by weight malt, starch 20% by weight, 25% by weight phase change coating agent, and 5% by weight phase induction catalyst prepared in Example 8 did not break at 10 °C for 0 to 48 hours.
  • the coating layer composed of 40% by weight of glucose, 20% by weight of starch, 35% by weight of phase change coating agent, and 5% by weight of phase induction catalyst prepared in Example 8 was not broken at 15 ° C. for 0 to 48 hours.
  • the coating layer made of 50% by weight malt, starch 20% by weight, 25% by weight phase change coating agent, and 5% by weight phase induction catalyst prepared in Example 8 did not break at 20 °C 0 to 48 hours.
  • the coating layer composed of 50% by weight malt, starch 30% by weight, 25% by weight phase change coating agent, and 5% by weight phase induction catalyst prepared in Example 8 did not break at 30 °C for 0 to 48 hours.
  • the lactic acid bacteria liquid 32 is preferably mixed with the medium powder or the medium liquid in the lactic acid bacteria liquid containing distilled water as well as the pure lactic acid bacteria liquid containing distilled water.
  • the lactic acid bacteria powder 30 or the lactic acid bacteria liquid 32 is preferably mixed with the medium.
  • lactic acid bacteria are similar to the rotted food poisoning bacteria and growth patterns, and form organic acids during growth to drop the pH, it is preferable that the microorganisms are harmless to the human body and the environment.
  • the decaying food poisoning bacteria E. coli O-157 or Salmonellane is preferred.
  • the lactic acid bacteria for decay check of the storage material is preferably selected from the group consisting of not only lactic acid bacteria, but also yeast, mold, and preferably Lactobacillus acidophilus.
  • the medium is preferably a composition in which the pH of the medium is lowered by the organic acid generated at the same time as the growth of lactic acid bacteria, MRS, medium containing 25% diluted MRS medium, the minimum medium and glucose 100 g / L It is preferable that it is any one selected from the group which consists of a minimum medium, and it is preferable that MRS medium is a medium diluted to 25%.
  • the lactic acid bacteria powder is preferably a mixed powder of lactic acid bacteria powder and medium powder, it is preferable to use a mixture of lyophilized lactic acid bacteria and medium powder in a ratio of 0.01 ⁇ 0.1: 99.99 ⁇ 99.9, lyophilized lactic acid bacteria and medium powder It is preferable to use the mixture in the ratio of 0.05 ⁇ 0.1: 99.95 ⁇ 99.9, it is preferable to use a mixture of lyophilized lactic acid bacteria and medium powder in the ratio of 0.1: 99.9, and further include a fungal growth inhibitor desirable.
  • the fungal growth inhibitor is preferably selected from the group consisting of antifungal polyenes (polyene antifungals), nystatin, amphotericin B (amphotericin B), pimaricin (pimaricin).
  • Lactobacillus acidophilus which is a lactic acid bacterium
  • the pH was lowered due to the growth and production of lactic acid, and the pH was lowered up to 3 weeks.
  • the lactic acid bacteria were incubated with pH indicators such as pH indicators, metal soap particles, and pH sensitive polymer particles.
  • the pH of the lactic acid bacteria decreased and the color of the medium changed to indicate that the acid was acidic.
  • the acidic color was maintained even when the medium was adjusted to neutral.
  • the label of the present invention is cryopreserved, in order for the label to be effectively used, the bacterium should be able to grow even when the temperature is raised even at the freezing temperature (eg, -20 ° C).
  • bacteria when the temperature falls below the freezing point while the label is used in the storage space, bacteria must survive even below the freezing point in order for the label to work properly. Above all, if stored at low temperatures for a long time, lactic acid bacteria must survive even if they do not grow. Even if the lactic acid bacteria were cultured after freezing storage, the cultures could be cultured like the microorganisms cultured without freezing storage or cultured after cold storage and the pH of the culture solution was dropped at the same time as the culture.
  • lyophilized lactic acid bacteria should be used less than 0.1% (W / V) has no effect on the determination of color due to turbidity by other materials, At least 10,000 CFU / ml, the growth of bacteria occurs within 1-2 days.
  • corruption of the storage material may be performed by comparing the color of the pH indicator according to the culture state of the microorganism.
  • the label of the present invention can be usefully used as a label for determining the perishability of the storage material according to the accumulation of storage temperature change.
  • the lactic acid bacteria are similar to the growth pattern of Escherichia coli O-157, Salmonella, which is a decaying food poisoning bacterium. , Yeast, fungi, and the like, and most preferably Lactobacillus acidophilus is used.
  • the medium composition is determined so that the pH of the medium is lowered by the organic acid generated at the same time as the growth of the lactic acid bacteria, and preferably, the medium, the minimum medium and 100 g / L of the MRS and the MRS medium diluted to 25% Minimal medium including, etc. may be used, more preferably, a medium in which MRS medium is diluted to 25% may be used.
  • the pH of the medium is neutral, and when there are many amino acid components in the medium, the pH is increased by ammonia generated while being degraded by lactic acid bacteria.
  • the lactic acid bacteria produce an organic acid in proportion to the degree of growth, and the organic acid becomes acidic by dropping the pH of the medium.
  • a color change indicator is added to the medium, and the color changes as the lactic acid bacteria are changed to the same temperature as the storage material, which can grow if there is a decay bacteria in the storage material.
  • the lactic acid bacteria mixed powder is mixed with the lyophilized lactic acid bacteria and the medium powder at a ratio of 0.01 to 0.1: 99.9 to 99.99, preferably at a ratio of 0.05 to 0.1: 99.9 to 99.95, and most preferably at a ratio of 0.1: 99.9. It is preferable to use.
  • lactic acid bacteria in the form of lyophilized powder pre-mixed with the medium powder and prepared in a state separated from water.
  • the culture of various bacteria can be suppressed, but it is not a sterile situation, and the fungus is mixed.
  • a fungal growth inhibitor may be additionally added to the mixed powder of the present invention, and as a fungal growth inhibitor, antifungal polyene (polyene antifungals), nystatin, and amphotericin B (amphotericin) B), pimaricin and the like can be used.
  • the pH indicator may be used as the indicator of the color change in neutral and acidic, preferably methyl red, methyl blue, bromocresol Green-Methyl Red, bromocre Bromocresol Green and Bremophenol Blue can be used.
  • the pH may be neutral when the pH rises again due to growth of other contaminants, and thus the color should not change even when the pH rises. Once a color changes due to a low pH, a method is needed to maintain that color.
  • the pH indicator liquid 22 is preferably a pH indicator liquid containing distilled water.
  • the pH indicator may be used as the general pH indicator as well as metal soap particles and pH sensitive polymer particles.
  • the pH indicator may be used as the indicator of the color change in neutral and acidic, preferably methyl red, methyl blue, bromocresol Green-Methyl Red, bromocre Bromocresol Green and Bremophenol Blue can be used.
  • the pH indicator liquid 22 is preferably a pH indicator liquid containing distilled water.
  • the pH indicator is prepared by dissolving 0.1 g of methyl red and 0.1 g of methylene blue in 190 ml of ethanol and filling it with water to make 200 ml. Any color-changing indicator can be applied when changing from neutral to acidic.
  • the pH indicator is preferably metal soap particles or pH-sensitive polymer particles, and the metal soap particles are preferably in the form of metal soap granules by a saponification reaction of a pigment and oleic acid with divalent ions.
  • the metal soap particles are made of granulated metal soap by reacting pigments and oleic acid with divalent ions such as calcium, and when the pH is lowered with organic acids, the metal soap is decomposed again to disperse the dye. This becomes the color of the pigment as a whole. This reaction maintains the color in the label as the dye does not spontaneously repel the metal soap even if the pH changes to neutral over time as the components in the label change over time.
  • the metal soap is a saponification reaction of the pigment and oleic acid with calcium divalent ions to produce a metal soap granule form.
  • 10 mg of D & C Red No. 6 Barium-lake, 15 g of oleic acid, 2.204 g of Ca (OH) 2, 5.67 g of H 2 O and 0.0375 g of lipase were reacted at 60 ° C. at 480 rpm for 2.5 hours, and then finely ground.
  • the pH indicator is preferably a pH-sensitive polymer, chitosan, polylysine, polyethyleneimine (PEI), diethylaminoethyl-dextran (DEAE-dextran) and poly (amido) Amine) dendrimers [poly (amidoamine) (PAMAM) dendrimers] is preferably selected from the group consisting of.
  • the pH-sensitive polymer particles are prepared in a form in which the pigment is collected as a basic polymer in which the polymer is expanded at low pH, and when the pH is lowered with the organic acid, the pigment trapped in the polymer particles is released into the medium to have a color of the pigment. Even if it is neutral again, the color of the pigment can be maintained since the pigment does not enter the shrinked polymer particles.
  • the basic polymer includes chitosan, polylysine, polyethyleneimine (PEI), diethylaminoethyl-dextran (DEAE-dextran), and poly (amidoamine) dendrimer [poly (amidoamine). (PAMAM) dendrimers] and the like can be used.
  • the pigment in the present invention D & C Red No. 6 Barium-lake, a red organic dye, an inorganic dye, and the like may be used.
  • pH-sensitive polymers there are many kinds of pH-sensitive polymers in the present invention and all of the basic polymers that expand at low pH can be applied.
  • 0.5 g of chitosan can be dissolved in 50 ml of 0.1% (V / V) acetic acid and EDTA can be added to 3-80% (Wt%) of chitosan to increase strength.
  • 1-5 mg of pigment (D & C Red No. 6 Barium-lake; Warner-Jenkinson, USA) is added and the pH is neutralized with 5M NaOH.
  • EDAC [1-Ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride; Sigma, USA] is added to 10-100 mM. After stirring for 4 hours at room temperature, washed twice with distilled water, washed twice with 0.5 M NaCl, 0.025M NaOH and twice with 0.05M NaOH, and then washed once with distilled water to be refrigerated.
  • the label prepared to include the lyophilized organic acid and the pH indicator may be exposed to various temperatures, and then the corruption of the storage material may be determined by comparing the colors of the pH indicator.
  • the label of the present invention can be usefully used as a label for determining the perishability of the storage material according to the accumulation of storage temperature change.
  • the label of the present invention may be manufactured in a form that can be adhered to the outside of the food storage container. It is manufactured in the form of a label that is directly attached to the outer container of the storage material that is perished by temperature, so that the change in the actual temperature of the storage material may be measured, not the temperature of the storage space during storage and transportation.
  • the pH indicator, the metal soap particles, and the pH sensitive polymer particles were changed in pH at 30 ° C. at concentrations of 0.0001%, 1%, and 1%, respectively. And the color change of the pH marker. At the end, calibrate the pH to neutral and check the color change.
  • the pH indicator which was initially green, turns purple and red to maintain its color, and the color remains red even when the metal soap particles or pH-sensitive polymer particles remain neutral.
  • the present invention attaches the label to the outer container of the storage material, and selectively dissolves at the outer temperature of the coating layer -10 to 40 °C dissolved when the storage material is decayed as the storage temperature is increased to -10 to 40 °C as the storage period elapses
  • the coating layer 50 is dissolved, the color of the pH indicator is discolored, and when the acidic index is observed by observing the color of the discolored label, it may be determined that the storage material may be decayed.
  • the label prepared to confirm the color change according to the change of the storage temperature is -10 °C, 0 °C, 4 °C, 5 °C, 10 °C, 15 °C, 20 °C, 25 °C, 30 °C, 35 °C, 37
  • the indicator changes color, the possibility of corruption can be determined based on the color change.
  • the surface coating layer 40 of the label is PE (polyethylene), PP (poly propylene), PS (Poly styrene), PET (Poly Ethylene Telephthalate), etc. to protect the internal material against the pressure applied during adhesion It is preferable to use opaque white to make the material in the form of a film and to avoid confusion with the color of the storage material.

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Abstract

The present invention relates to a functional label, having a lactobacillus coating for detecting external impact, for checking the freshness of a storage material and, more specifically, to a functional label having attached on the upper part thereof a pH indicator layer and a lactobacillus core coating layer, thereby enabling checking of the freshness of a storage material when a coating layer for detecting changes in temperature cracks in external temperature from -10°C to 40°C.

Description

저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨Lactobacillus coating functional label for external shock detection to check freshness of storage materials
본 발명은 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨에 관한 것으로서, 더욱 상세하게는 라벨의 상부에 pH 지시약층과 유산균 코팅코아층이 부착되어 있어 외부 충격으로 코팅층이 깨지는 경우 저장물질의 신선도 체크를 할 수 있는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨에 관한 것이다.The present invention relates to a functional label for external shock detection lactic acid bacteria coating for checking the freshness of a storage material, and more particularly, a pH indicator layer and a lactic acid bacterium coating core layer are attached to an upper portion of the label, so that the coating layer is broken by an external impact. The present invention relates to a lactic acid bacteria coating functional label for external shock detection for freshness check of storage materials that can be checked for freshness.
식품과 같이 부패하기 쉬운 물질은 냉장상태로 보관되거나 운반된다. 이때, 저장온도가 변하거나 식품이 외부에 노출될 경우 부패가 발생할 수 있다. 식품이 방출 이후 최종 사용되기 전까지 제대로 냉장 보관 상태가 유지되었는지를 판단할 수 있다면 부패균의 성장에 따른 식중독 등을 사전에 예방할 수 있고 식품의 안전관리에 많은 도움이 될 수 있다.Perishable substances such as foods are stored or transported refrigerated. At this time, if the storage temperature is changed or the food is exposed to the outside may cause corruption. If it is possible to determine whether the food has been properly stored in the refrigerator until its final use after release, it can prevent food poisoning due to the growth of decayed bacteria and can be helpful for food safety management.
식품 저장시의 외부 공기의 시간에 따른 변화의 이력을 측정 및 표시하는 방법은 화학적 및 물리적으로 가능하며 외부 환경의 온도 변화로서 시간 및 정도를 물리적으로 측정하는 것은 가능하지만 저장용기 내의 물질로서 액상의 식품의 온도가 어떻게 변했는가를 개별적으로 측정하는 것은 저장공간의 상태, 보관용기의 열전달 정도의 다양한 인자 때문에 더욱 힘들다.The method of measuring and displaying the history of the change of the external air with time during food storage is chemically and physically possible, and it is possible to physically measure the time and degree as the temperature change of the external environment, but the liquid food as the substance in the storage container. The individual measurement of how the temperature of the furnace has changed is more difficult due to various factors such as the condition of the storage space and the degree of heat transfer in the container.
저장된 식품의 신선도 여부를 측정 및 판단하기 위해 저장 온도 및 노출 시간 등을 판단하는 것은 더욱더 어렵다. 물리적인 온도 변화의 측정보다 더욱 어려운 것은 다양한 형태의 온도변화에 의해 궁극적으로 부패균이 성장하였는지를 생물학적으로 판단하기는 쉽지 않다. 부패균 성장에 소요되는 시간 및 온도는 예측할 수 있지만 실제 식품 저장 공간에서의 지속적으로 변화하는 온도의 상태와 각각 다른 노출시간에 의해 부패균이 실제 성장할지를 물리, 화학적으로 예측할 수는 없다.In order to measure and determine the freshness of the stored food, it is more difficult to determine the storage temperature and the exposure time. What is more difficult than measuring physical temperature changes is that it is not easy to biologically determine whether the decayed bacteria eventually grew by various types of temperature changes. The time and temperature required to grow rot can be predicted, but it is not possible to predict physically and chemically whether the rot will actually grow due to the constantly changing temperature conditions in the food storage space and the different exposure times.
동결건조된 유산균 및 배지 분말 및 pH 지시약 및 증류수를 포함하는 식품 저장 용기의 외부에 접착될 수 있는 형태의 라벨을 제작하고 라벨이 온도변화 누적에 따른 저장물질의 부패가능성 판단에 사용될 수 있다.A label may be prepared in a form that can be adhered to the outside of the food storage container including the lyophilized lactic acid bacteria and the medium powder and the pH indicator and the distilled water, and the label may be used to determine the perishability of the storage material due to the accumulation of temperature change.
본 발명의 목적은 종래의 문제점을 개선하여 저장 온도변화의 누적에 따른 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨을 제공하는 것이다.It is an object of the present invention to improve the conventional problems to provide a functional label for external shock detection lactic acid bacteria coating for checking the freshness of the storage material according to the accumulation of storage temperature changes.
본 발명은 라벨의 상부에 pH 지시약층과 유산균 코팅코아층이 부착되어 있어 외부 충격으로 코팅층이 깨지는 경우 저장물질의 신선도 체크를 할 수 있는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨에 관한 것이다.The present invention relates to a pH indicator layer and a lactic acid bacteria coating core layer attached to the upper part of the label, and to an external shock detection lactic acid bacterium coating functional label for checking the freshness of the storage material when the coating layer is broken by an external impact. will be.
본 발명의 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨은 식품 저장 용기의 외부에 접착가능 하며 저장물질과 같은 온도변화를 받은 유산균이 성장하면 색이 변하게 되어 색 변화를 관찰함으로써 저장물질의 신선도 여부를 판단할 수 있다.The functional label of the external shock detection lactic acid bacterium coating for checking the freshness of the storage material of the present invention can be adhered to the outside of the food storage container, and the color changes when the lactic acid bacteria are grown under the same temperature change as the storage material. You can judge the freshness.
도 1 내지 도 22는 본 발명의 저장물질의 신선도 체크용 기능성 라벨을 나타낸 분해단면도.1 to 22 are exploded cross-sectional view showing a functional label for checking the freshness of the storage material of the present invention.
도 1 내지 도 4와 같이 본 발명은 라벨(10)의 상부표면에 부착되어 있는 pH 지시약층(24)과 상기 pH 지시약층(24)의 상부에 부착된 유산균 코팅코아층(38); 및1 to 4, the present invention includes a pH indicator layer 24 attached to an upper surface of the label 10 and a lactic acid bacteria coating core layer 38 attached to an upper portion of the pH indicator layer 24; And
라벨(10)의 상부표면에 부착되어 있는 유산균 코팅코아층(38)과 상기 유산균 코팅코아층(38)의 상부에 부착된 pH 지시약층(24); 중에서 선택된 어느 하나의 2층으로 이루어져 있으며,A lactic acid bacteria coating core layer 38 attached to the upper surface of the label 10 and a pH indicator layer 24 attached to the upper portion of the lactic acid bacteria coating core layer 38; It consists of any two layers selected from,
상기 pH 지시약층(24)은 pH 지시약 분말(20) 및 pH 지시약 액체(22) 중에서 선택된 어느 하나의 지시약으로 이루어져 있고, The pH indicator layer 24 is composed of any one indicator selected from the pH indicator powder 20 and the pH indicator liquid 22,
상기 유산균 코팅코아층(38)은 유산균 코팅코아(36)의 내부에 유산균 액체(32)가 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 유산균 액체층의 외부에 코팅된 다수개의 유산균 코팅코아(36)로 이루어진 것을 특징으로 하는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨에 관한 것이다.The lactic acid bacteria coating core layer 38 is a lactic acid bacteria liquid 32 is embedded in the lactic acid bacteria coating core 36, the outer layer of the lactic acid bacteria liquid layer as a coating layer 50 for the external shock detection that the coating layer is broken by an external impact. The present invention relates to a label for labeling a lactic acid bacteria functional label for external shock detection of freshness of a storage material, which comprises a plurality of coated lactic acid bacteria coating cores 36.
본 발명에서 외부충격감지 유산균 코팅 기능성 라벨은 2개의 층 상부에 표면코팅층(40)이 추가로 코팅된 것을 특징으로 한다.In the present invention, the outer shock detection lactic acid bacteria functional label is characterized in that the surface coating layer 40 is further coated on the two layers.
도 1 내지 도 4와 같이 본 발명은 라벨(10)의 상부표면에 부착되어 있는 pH 지시약층(24)과 상기 pH 지시약층(24)의 상부에 부착된 유산균 코팅코아층(38); 및 라벨(10)의 상부표면에 부착되어 있는 유산균 코팅코아층(38)과 상기 유산균 코팅코아층(38)의 상부에 부착된 pH 지시약층(24); 중에서 선택된 어느 하나의 2층으로 이루어져 있으며,1 to 4, the present invention includes a pH indicator layer 24 attached to an upper surface of the label 10 and a lactic acid bacteria coating core layer 38 attached to an upper portion of the pH indicator layer 24; And a pH indicator layer 24 attached to an upper portion of the lactic acid bacteria coating core layer 38 and the lactic acid bacteria coating core layer 38 attached to the upper surface of the label 10. It consists of any two layers selected from,
상기 pH 지시약층(24)은 pH 지시약 액체(22)로 이루어져 있고, The pH indicator layer 24 is composed of a pH indicator liquid 22,
상기 유산균 코팅코아층(38)은 유산균 코팅코아(36)의 내부에 유산균 분말(30)이 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 유산균 분말층의 외부에 코팅된 다수개의 유산균 코팅코아(36)로 이루어진 것을 특징으로 하는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨에 관한 것이다.The lactic acid bacteria coating core layer 38 is a lactic acid bacteria powder 30 is embedded in the lactic acid bacteria coating core 36, the coating layer for the external impact detection layer 50 is broken by an external impact on the outside of the lactic acid bacteria powder layer. The present invention relates to a label for labeling a lactic acid bacteria functional label for external shock detection of freshness of a storage material, which comprises a plurality of coated lactic acid bacteria coating cores 36.
도 5 내지 도 7과 같이 본 발명은 라벨(10)의 상부표면에 부착되어 있는 pH 지시약 코팅코아층(28)과 상기 pH 지시약 코팅코아층(28)의 상부에 부착된 유산균층(34); 및 라벨(10)의 상부표면에 부착되어 있는 유산균층(34)과 상기 유산균 코팅코아층(38)의 상부에 부착된 pH 지시약 코팅코아층(28); 중에서 선택된 어느 하나의 2층으로 이루어져 있으며,5 to 7, the present invention includes a pH indicator coating core layer 28 attached to an upper surface of the label 10 and a lactic acid bacteria layer 34 attached to an upper portion of the pH indicator coating core layer 28; And a pH indicator coating core layer 28 attached to an upper portion of the lactic acid bacteria layer 34 and the lactic acid bacteria coating core layer 38 attached to the upper surface of the label 10. It consists of any two layers selected from,
상기 유산균층(34)은 유산균 분말(30) 및 유산균 액체(32) 중에서 선택된 어느 하나의 유산균으로 이루어져 있고, The lactic acid bacteria layer 34 is composed of any one lactic acid bacteria selected from lactic acid bacteria powder 30 and the liquid lactic acid bacteria 32,
상기 pH 지시약 코팅코아층(28)은 pH 지시약 코팅코아(26)의 내부에 pH 지시약 액체(22)가 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 pH 지시약 액체층의 외부에 코팅된 다수개의 pH 지시약 코팅코아(26)로 이루어진 것을 특징으로 하는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨에 관한 것이다.The pH indicator coating core layer 28 is a pH indicator liquid 22 is embedded in the pH indicator coating core 26, the pH indicator liquid as the coating layer 50 for the external shock detection that the coating layer is broken by an external impact. The present invention relates to an external shock detection lactic acid bacterium coating functional label for checking the freshness of a storage material, comprising a plurality of pH indicator coating cores 26 coated on the outside of the layer.
본 발명의 라벨은 상기 pH 지시약 코팅코아층(28)과 유산균층(34)의 2개 층 상부에 표면코팅층(40)이 추가로 코팅된 것을 특징으로 한다.The label of the present invention is characterized in that the surface coating layer 40 is further coated on top of the two layers of the pH indicator coating core layer 28 and the lactic acid bacteria layer 34.
도 5 내지 도 6과 같이 본 발명은 라벨(10)의 상부표면에 부착되어 있는 pH 지시약 코팅코아층(28)과 상기 pH 지시약 코팅코아층(28)의 상부에 부착된 유산균층(34); 및 라벨(10)의 상부표면에 부착되어 있는 유산균층(34)과 상기 유산균 코팅코아층(38)의 상부에 부착된 pH 지시약 코팅코아층(28); 중에서 선택된 어느 하나의 2층으로 이루어져 있으며,5 to 6, the present invention includes a pH indicator coating core layer 28 attached to the upper surface of the label 10 and a lactic acid bacteria layer 34 attached to the top of the pH indicator coating core layer 28; And a pH indicator coating core layer 28 attached to an upper portion of the lactic acid bacteria layer 34 and the lactic acid bacteria coating core layer 38 attached to the upper surface of the label 10. It consists of any two layers selected from,
상기 유산균층(34)은 유산균 분말(30)로 이루어져 있고, The lactic acid bacteria layer 34 is made of lactic acid bacteria powder 30,
상기 pH 지시약 코팅코아층(28)은 pH 지시약 코팅코아(26)의 내부에 pH 지시약 액체(22)가 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 pH 지시약 액체층의 외부에 코팅된 다수개의 pH 지시약 코팅코아(26)로 이루어진 것을 특징으로 하는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨에 관한 것이다.The pH indicator coating core layer 28 is a pH indicator liquid 22 is embedded in the pH indicator coating core 26, the pH indicator liquid as the coating layer 50 for the external shock detection that the coating layer is broken by an external impact. The present invention relates to an external shock detection lactic acid bacterium coating functional label for checking the freshness of a storage material, comprising a plurality of pH indicator coating cores 26 coated on the outside of the layer.
본 발명의 라벨은 상기 pH 지시약 코팅코아층(28)과 유산균층(34)의 2개 층 상부에 표면코팅층(40)이 추가로 코팅된 것을 특징으로 한다.The label of the present invention is characterized in that the surface coating layer 40 is further coated on top of the two layers of the pH indicator coating core layer 28 and the lactic acid bacteria layer 34.
도 7 내지 도 8과 같이 본 발명은 라벨(10)의 상부표면에 부착되어 있는 pH 지시약 코팅코아층(28)과 상기 pH 지시약 코팅코아층(28)의 상부에 부착된 유산균 코팅코아층(38); 및 라벨(10)의 상부표면에 부착되어 있는 유산균 코팅코아층(38)과 상기 유산균 코팅코아층(38)의 상부에 부착된 pH 지시약 코팅코아층(28); 중에서 선택된 어느 하나의 2층으로 이루어져 있으며,As shown in FIG. 7 to FIG. 8, the present invention shows a pH indicator coating core layer 28 attached to the upper surface of the label 10 and a lactic acid bacteria coating core layer 38 attached to the top of the pH indicator coating core layer 28. ); And a pH indicator coating core layer 28 attached to the top of the lactic acid bacteria coating core layer 38 and the lactic acid bacteria coating core layer 38 attached to the upper surface of the label 10. It consists of any two layers selected from,
상기 pH 지시약 코팅코아층(28)은 pH 지시약 코팅코아(26)의 내부에 pH 지시약 분말(20) 및 pH 지시약 액체(22) 중에서 선택된 어느 하나의 지시약이 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 pH 지시약층의 외부에 코팅된 다수개의 pH 지시약 코팅코아(26)로 이루어져 있으며,The pH indicator coating core layer 28 has any one indicator selected from the pH indicator powder 20 and the pH indicator liquid 22 inside the pH indicator coating core 26, and the coating layer is broken by an external impact. The external shock detection coating layer 50 is composed of a plurality of pH indicator coating core 26 coated on the outside of the pH indicator layer,
상기 유산균 코팅코아층(38)은 유산균 코팅코아(36)의 내부에 유산균 액체(32)가 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 유산균 액체층의 외부에 코팅된 다수개의 유산균 코팅코아(36)로 이루어진 것을 특징으로 하는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨에 관한 것이다.The lactic acid bacteria coating core layer 38 is a lactic acid bacteria liquid 32 is embedded in the lactic acid bacteria coating core 36, the outer layer of the lactic acid bacteria liquid layer as a coating layer 50 for the external shock detection that the coating layer is broken by an external impact. The present invention relates to a label for labeling a lactic acid bacteria functional label for external shock detection of freshness of a storage material, which comprises a plurality of coated lactic acid bacteria coating cores 36.
본 발명의 라벨은 상기 pH 지시약 코팅코아층(28)과 유산균 코팅코아층(38)의 2개 층 상부에 표면코팅층(40)이 추가로 코팅된 것을 특징으로 한다.The label of the present invention is characterized in that the surface coating layer 40 is further coated on top of two layers of the pH indicator coating core layer 28 and the lactic acid bacteria coating core layer 38.
도 7 내지 도 8과 같이 본 발명은 라벨(10)의 상부표면에 부착되어 있는 pH 지시약 코팅코아층(28)과 상기 pH 지시약 코팅코아층(28)의 상부에 부착된 유산균 코팅코아층(38); 및As shown in FIG. 7 to FIG. 8, the present invention shows a pH indicator coating core layer 28 attached to the upper surface of the label 10 and a lactic acid bacteria coating core layer 38 attached to the top of the pH indicator coating core layer 28. ); And
라벨(10)의 상부표면에 부착되어 있는 유산균 코팅코아층(38)과 상기 유산균 코팅코아층(38)의 상부에 부착된 pH 지시약 코팅코아층(28); 중에서 선택된 어느 하나의 2층으로 이루어져 있으며,A lactic acid bacteria coating core layer 38 attached to the upper surface of the label 10 and a pH indicator coating core layer 28 attached to the upper portion of the lactic acid bacteria coating core layer 38; It consists of any two layers selected from,
상기 pH 지시약 코팅코아층(28)은 pH 지시약 코팅코아(26)의 내부에 pH 지시약 액체(22)이 내장되어 있으며, 상기 pH 지시약층의 외부에 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 코팅된 다수개의 pH 지시약 코팅코아(26)로 이루어져 있으며,The pH indicator coating core layer 28 has a pH indicator liquid 22 embedded in the pH indicator coating core 26, the coating layer for external impact detection that the coating layer is broken by an external impact on the outside of the pH indicator layer ( 50) a plurality of pH indicator coating core (26) coated with
상기 유산균 코팅코아층(38)은 유산균 코팅코아(36)의 내부에 유산균 분말(32)이 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 유산균 분말층의 외부에 코팅된 다수개의 유산균 코팅코아(36)로 이루어진 것을 특징으로 하는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨에 관한 것이다.The lactobacillus coating core layer 38 is a lactic acid bacteria powder 32 is embedded in the lactic acid bacteria coating core 36, the outer layer of the lactic acid bacteria powder layer as an external impact detection coating layer 50 is broken by an external impact. The present invention relates to a label for labeling a lactic acid bacteria functional label for external shock detection of freshness of a storage material, which comprises a plurality of coated lactic acid bacteria coating cores 36.
본 발명의 라벨은 상기 pH 지시약 코팅코아층(28)과 유산균 코팅코아층(38)의 2개 층 상부에 표면코팅층(40)이 추가로 코팅된 것을 특징으로 한다.The label of the present invention is characterized in that the surface coating layer 40 is further coated on top of two layers of the pH indicator coating core layer 28 and the lactic acid bacteria coating core layer 38.
본 발명은 라벨(10)의 상부표면에 pH 지시약 코팅코아층(28)과 유산균 코팅코아층(38)의 혼합층이 부착되어 있으며,In the present invention, a mixed layer of the pH indicator coating core layer 28 and the lactic acid bacteria coating core layer 38 is attached to the upper surface of the label 10,
상기 pH 지시약 코팅코아층(28)은 pH 지시약 코팅코아(26)의 내부에 pH 지시약 분말(20) 및 pH 지시약 액체(22) 중에서 선택된 어느 하나의 지시약이 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 pH 지시약층의 외부에 코팅된 다수개의 pH 지시약 코팅코아(26)로 이루어져 있고,The pH indicator coating core layer 28 has any one indicator selected from the pH indicator powder 20 and the pH indicator liquid 22 inside the pH indicator coating core 26, and the coating layer is broken by an external impact. It consists of a plurality of pH indicator coating core (26) coated on the outside of the pH indicator layer to the external impact detection coating layer (50),
상기 유산균 코팅코아층(38)은 유산균 코팅코아(36)의 내부에 유산균 액체(32)가 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 유산균 액체층의 외부에 코팅된 다수개의 유산균 코팅코아(36)로 이루어진 것을 특징으로 하는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨에 관한 것이다.The lactic acid bacteria coating core layer 38 is a lactic acid bacteria liquid 32 is embedded in the lactic acid bacteria coating core 36, the outer layer of the lactic acid bacteria liquid layer as a coating layer 50 for the external shock detection that the coating layer is broken by an external impact. The present invention relates to a label for labeling a lactic acid bacteria functional label for external shock detection of freshness of a storage material, which comprises a plurality of coated lactic acid bacteria coating cores 36.
본 발명의 라벨은 혼합층의 상부에 표면코팅층(40)이 추가로 코팅된 것을 특징으로 한다.The label of the present invention is characterized in that the surface coating layer 40 is further coated on top of the mixed layer.
도 9 내지 도 10과 같이 본 발명은 라벨(10)의 상부표면에 pH 지시약 코팅코아(26)과 유산균 코팅코아(36)의 혼합층이 부착되어 있으며,9 to 10, the present invention is attached to the mixed layer of the pH indicator coating core 26 and lactic acid bacteria coating core 36 on the upper surface of the label 10,
상기 pH 지시약 코팅코아(26)는 pH 지시약 코팅코아(26)의 내부에 pH 지시약 분말(20) 및 pH 지시약 액체(22) 중에서 선택된 어느 하나의 지시약이 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 pH 지시약층의 외부에 코팅되어 있고,The pH indicator coating core 26 has any one indicator selected from the pH indicator powder 20 and the pH indicator liquid 22 inside the pH indicator coating core 26, the outer coating layer is broken by an external impact. It is coated on the outside of the pH indicator layer with a shock-sensitive coating layer 50,
상기 유산균 코팅코아(36)는 유산균 코팅코아(36)의 내부에 유산균 액체(32)가 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 유산균 액체층의 외부에 코팅되어 있는 것을 특징으로 하는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨에 관한 것이다.The lactic acid bacteria coating core 36 is a lactic acid bacteria liquid 32 is embedded in the lactic acid bacteria coating core 36, the coating layer 50 for the external shock detection that the coating layer is broken by an external impact is coated on the outside of the lactic acid bacteria liquid layer. It relates to a functional label for external shock detection lactic acid bacteria coating for checking the freshness of the storage material, characterized in that it is.
본 발명의 라벨은 혼합층의 상부에 표면코팅층(40)이 추가로 코팅된 것을 특징으로 한다.The label of the present invention is characterized in that the surface coating layer 40 is further coated on top of the mixed layer.
도 9 내지 도 10과 같이 본 발명은 라벨(10)의 상부표면에 pH 지시약 코팅코아(26)과 유산균 코팅코아(36)의 혼합층이 부착되어 있으며,9 to 10, the present invention is attached to the mixed layer of the pH indicator coating core 26 and lactic acid bacteria coating core 36 on the upper surface of the label 10,
상기 pH 지시약 코팅코아(26)는 pH 지시약 코팅코아(26)의 내부에 pH 지시약 액체(22)가 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 pH 지시약층의 외부에 코팅되어 있고,The pH indicator coating core 26 is a pH indicator liquid 22 is built in the pH indicator coating core 26, the coating layer for the external shock detection coating layer 50 is broken by an external impact of the pH indicator layer of Coated on the outside,
상기 유산균 코팅코아(36)는 유산균 코팅코아(36)의 내부에 유산균 분말(30)이 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 유산균 액체층의 외부에 코팅되어 있는 것을 특징으로 하는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨에 관한 것이다.The lactic acid bacteria coating core 36 is a lactic acid bacterium coating core 36 is embedded in the lactic acid bacteria powder 30, the coating layer 50 for the external shock detection that the coating layer is broken by an external impact is coated on the outside of the lactic acid bacteria liquid layer. It relates to a functional label for external shock detection lactic acid bacteria coating for checking the freshness of the storage material, characterized in that it is.
본 발명의 라벨은 상기 혼합층의 상부에 표면코팅층(40)이 추가로 코팅된 것을 특징으로 한다.The label of the present invention is characterized in that the surface coating layer 40 is further coated on top of the mixed layer.
도 11 내지 도 12와 같이 본 발명은 라벨(10)의 상부표면에 pH 지시약 분말(20)과 유산균 코팅코아(36)의 혼합층이 부착되어 있으며,11 to 12 is a mixed layer of the pH indicator powder 20 and the lactic acid bacteria coating core 36 is attached to the upper surface of the label 10,
상기 유산균 코팅코아(36)는 유산균 코팅코아(36)의 내부에 유산균 액체(32)가 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 유산균 액체층의 외부에 코팅되어 있는 것을 특징으로 하는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨에 관한 것이다.The lactic acid bacteria coating core 36 is a lactic acid bacteria liquid 32 is embedded in the lactic acid bacteria coating core 36, the coating layer 50 for the external shock detection that the coating layer is broken by an external impact is coated on the outside of the lactic acid bacteria liquid layer. It relates to a functional label for external shock detection lactic acid bacteria coating for checking the freshness of the storage material, characterized in that it is.
본 발명의 라벨은 상기 혼합층의 상부에 표면코팅층(40)이 추가로 코팅된 것을 특징으로 한다.The label of the present invention is characterized in that the surface coating layer 40 is further coated on top of the mixed layer.
도 13 내지 도 14와 같이 본 발명은 라벨(10)의 상부표면에 pH 지시약 코팅코아(26)과 유산균 분말(30)의 혼합층이 부착되어 있으며,13 to 14, the present invention is attached to the mixed layer of the pH indicator coating core 26 and lactic acid bacteria powder 30 on the upper surface of the label (10),
상기 pH 지시약 코팅코아(26)은 pH 지시약 코팅코아(26)의 내부에 pH 지시약 액체(22)가 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 pH 지시약층의 외부에 코팅되어 있는 것을 특징으로 하는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨에 관한 것이다.The pH indicator coating core 26 is a pH indicator liquid 22 is embedded in the pH indicator coating core 26, the coating layer for the external shock detection coating 50 is broken by an external impact of the pH indicator layer of The present invention relates to an external shock detection lactic acid bacterium coating functional label for checking the freshness of a storage material, which is coated on the outside.
본 발명의 라벨은 상기 혼합층의 상부에 표면코팅층(40)이 추가로 코팅된 것을 특징으로 한다.The label of the present invention is characterized in that the surface coating layer 40 is further coated on top of the mixed layer.
본 발명은 냉동만두와 같은 냉동식품이 냉동시킨 상태에서 외부온도가 -10 내지 40℃로 상승하는 경우 저장물질의 신선도 여부를 체크할 수 있는 외부충격감지 유산균 코팅 기능성 라벨에 관한 것이다.The present invention relates to a label for functional label for external shock detection lactic acid bacteria that can check the freshness of the storage material when the outside temperature rises to -10 to 40 ℃ in a frozen food such as frozen dumplings frozen.
본 발명에서 코팅층(50)은 냉동상태 또는 저온상태에서 형성된 코팅층이 저장물질에 라벨을 부착한 후 외부에서 1 내지 500 psi 정도의 충격이 가해지는 경우 코팅층(50)이 깨지는 외부충격 감지용 연질수지 코팅층이다.In the present invention, the coating layer 50 is a soft resin for detecting the external impact that the coating layer 50 is broken when the coating layer formed in a frozen state or a low temperature state is applied with a label of about 1 to 500 psi from the outside after the label is applied to the storage material. Coating layer.
본 발명에서 코팅층(50)은 외부에서 1 내지 500 psi 정도의 충격이 가해지는 경우 코팅층(50)이 깨지는 천연연질수지 코팅층 및 합성연질수지 코팅층을 전부 포함한다. 본 발명에서 합성연질수지 코팅층은 PE(poly ethylene), PP(poly propylene), PS(Poly styrene), PET(Poly Ethylene Telephthalate), 폴리에폭시수지, 아크릴수지, 페놀수지, 요소수지, 멜라민수지로 이루어진 군으로부터 선택되는 것이 바람직하다. In the present invention, the coating layer 50 includes all of the natural soft resin coating layer and the synthetic soft resin coating layer in which the coating layer 50 is broken when an impact of about 1 to 500 psi is applied from the outside. In the present invention, the synthetic soft resin coating layer is made of PE (poly ethylene), PP (poly propylene), PS (Poly styrene), PET (Poly Ethylene Telephthalate), poly epoxy resin, acrylic resin, phenol resin, urea resin, melamine resin It is preferably selected from the group.
본 발명에서 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)은 아크릴, 페놀수지, 요소 수지, 멜라민 수지 등이 있다. 또한 본 발명에서 냉동상태 또는 저온상태에서 형성된 코팅층(50)이 외부에서 1 내지 500 psi 정도의 충격이 가해지는 경우 코팅층(50)이 깨지면서 혼합분말과 혼합액이 섞여서 유산균이 배양할 수 있도록 경화성 수지를 사용하며 경화성 수지에는 아크릴, 페놀수지, 요소 수지, 멜라민 수지 등이 있다. In the present invention, the coating layer 50 for the external impact detection, which is broken by the external impact, includes acrylic, phenol resin, urea resin, melamine resin, and the like. In addition, in the present invention, when the coating layer 50 formed in the frozen state or the low temperature state is subjected to an impact of about 1 to 500 psi from the outside, the coating layer 50 is broken and the mixed powder and the mixed liquid are mixed so that the lactic acid bacteria can be cultured. Curable resins include acrylic, phenol resins, urea resins and melamine resins.
본 발명에서 라벨은 식품 저장 용기의 외부에 접착될 수 있는 형태로 제작되는 것이 바람직하며, 외부충격 감지용 코팅층(50)은 경화성 수지가 바람직하고, 상기 경화성 수지는 아크릴, 페놀수지, 요소 수지 및 멜라민 수지로 이루어진 군으로부터 선택되는 것이 바람직하다.In the present invention, the label is preferably produced in a form that can be adhered to the outside of the food storage container, the external impact detection coating layer 50 is preferably a curable resin, the curable resin is acrylic, phenol resin, urea resin and It is preferably selected from the group consisting of melamine resins.
본 발명에서 유산균 코팅코아(36)는 유산균 분말(30)을 코팅층(50)으로 냉동상태, 저온상태 또는 상온상태에서 코팅시킨 것이 바람직하다. 본 발명에서 유산균 코팅코아(36)는 유산균 액체(32)를 코팅층(50)으로 냉동상태, 저온상태 또는 상온상태에서 코팅시킨 것이 바람직하다. In the present invention, the lactic acid bacteria coating core 36 is preferably coated with lactic acid bacteria powder 30 in a frozen state, a low temperature state or a room temperature state with the coating layer 50. In the present invention, the lactic acid bacteria coating core 36 is preferably coated with the lactic acid bacteria liquid 32 in a frozen state, a low temperature state or a normal temperature state with the coating layer 50.
본 발명에서 pH 지시약 코팅코아(26)는 pH 지시약 분말(20)을 코팅층(50)으로 냉동상태, 저온상태 또는 상온상태에서 코팅시킨 것이 바람직하다. 본 발명에서 pH 지시약 코팅코아(26)는 pH 지시약 액체(22)를 코팅층(50)으로 냉동상태, 저온상태 또는 상온상태에서 코팅시킨 것이 바람직하다. In the present invention, the pH indicator coating core 26 is preferably coated with the pH indicator powder 20 in the freezing state, low temperature or room temperature state with the coating layer 50. In the present invention, the pH indicator coating core 26 is preferably coated with the pH indicator liquid 22 in the freezing state, low temperature or room temperature state with the coating layer 50.
본 발명의 라벨(10)은 부착 전에는 동결건조된 유산균과 배지 분말을 포함하는 혼합분말과 pH 지시약을 포함하는 혼합액이 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 분리되어 있다. The label 10 of the present invention is separated into a coating layer 50 for external shock detection, in which a mixed powder containing a lyophilized lactic acid bacterium and a medium powder and a mixed solution including a pH indicator are broken by an external impact.
본 발명의 라벨(10)은 양면테이프와 같은 접착부분을 벗기고 라벨(10)을 부착한 상태에서 외부온도가 -10 내지 40℃로 상승하는 경우 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)이 깨지는 온도변화 감지용 기능성 라벨이다.The label 10 of the present invention peels off an adhesive portion such as a double-sided tape, and when the external temperature rises to -10 to 40 ° C. in a state in which the label 10 is attached, the coating layer 50 for external impact detection that the coating layer is broken by an external impact. ) Is a functional label for detecting broken temperature changes.
또한 본 발명의 라벨(10)은 냉동상태 또는 저온상태에서 형성된 코팅층(50)이 외부에서 1 내지 500 psi 정도의 충격이 가해지는 경우 코팅층(50)이 깨지면서 혼합분말과 혼합액이 섞여서 유산균이 배양할 수 있는 상태가 된다. In addition, the label 10 of the present invention, when the coating layer 50 formed in a frozen state or a low temperature state is applied with an impact of about 1 to 500 psi from the outside, the coating layer 50 is broken and the mixed powder and the mixed solution are mixed so that lactic acid bacteria can be cultured. It becomes the state that I can.
본 발명은 라벨을 저장물질의 외부 용기에 부착한 후 외부충격이 가해지는 경우 코팅층(50)이 깨지고, 라벨의 색을 관찰하여 산성 색인 경우 부패 가능으로 판단하는 저장물질의 부패가능성 판단 방법을 제공한다.The present invention provides a method for determining the decay potential of the storage material to determine that the coating layer 50 is broken when the external impact is applied after the label is attached to the outer container of the storage material, and that the label is decomposable when the acid index is observed. do.
본 발명의 라벨은 다양한 온도에 노출한 후 코팅층(50)의 파쇄에 따른 pH 지시약의 색상을 비교함으로써 저장 물질의 부패판단을 수행할 수 있다. 본 발명의 방법은 저장 온도변화의 누적에 따른 저장물질의 부패가능성 판단에 유용하게 사용될 수 있다. 저장 중 외부온도의 변화의 누적으로 저장물질의 온도가 상승하거나 변화할 경우 저장물질의 외부 용기에 접착되어 있는 라벨 내의 유산균과 pH 지시약은 혼합된다. 본 발명에서 유산균에 의해 pH가 산성이 되면 pH 표지물질이 산성 색을 띠는지 여부를 확인함으로써 부패가능성을 판단할 수 있다.The label of the present invention can perform the decay determination of the storage material by comparing the color of the pH indicator according to the fracture of the coating layer 50 after exposure to various temperatures. The method of the present invention can be usefully used to determine the perishability of the storage material due to the accumulation of storage temperature changes. When the temperature of the storage material rises or changes due to the accumulation of changes in the external temperature during storage, the lactic acid bacteria and the pH indicator in the label adhered to the outer container of the storage material are mixed. When the pH becomes acidic by the lactic acid bacteria in the present invention, it is possible to determine the possibility of corruption by checking whether the pH labeling substance has an acidic color.
본 발명에서 코팅층(50)은 충격이 가해지는 경우 깨지는 충격변화 감지용 연질수지 코팅층이다. 본 발명에서 코팅층(50)은 천연연질수지 코팅층 및 합성연질수지 코팅층을 전부 포함한다. 본 발명에서 합성연질수지 코팅층은 당으로 이루어진 코팅층이 바람직하다. In the present invention, the coating layer 50 is a soft resin coating layer for shock change detection broken when an impact is applied. In the present invention, the coating layer 50 includes all of the natural soft resin coating layer and the synthetic soft resin coating layer. In the present invention, the synthetic soft resin coating layer is preferably a coating layer made of sugar.
또한 본 발명에서 코팅층(50)을 제조할 수 있는 성분은 식품첨가제 당 조성물이 바람직하다. 본 발명에서 코팅층(50)은 엿당, 포도당, 과당, 맥아당 및 설탕 중에서 선택하여 사용하는 것이 바람직하다. In addition, the component that can be prepared in the coating layer 50 in the present invention is preferably a food additive sugar composition. In the present invention, the coating layer 50 is preferably used to select from maltose, glucose, fructose, maltose and sugar.
본 발명의 코팅층에 사용되는 식품첨가제 당 조성물 중에서 단당류는 탄소수에 따라 2탄당, 3탄당, 4탄당, 5탄당 및 6탄당으로 분류되며, 단당류 중 중요한 단당류는 육탄당이다.In the food additive sugar composition used in the coating layer of the present invention, monosaccharides are classified into bisaccharides, trisaccharides, tetrasaccharides, pentoses, and hexasaccharides, depending on the number of carbons. Among the monosaccharides, an important monosaccharide is hexose.
포도당(glucose)은 체내 당 대사의 중심물질로서 생체계의 가장 기본적인 에너지 급원이며 채소나 과일에 많고 특히 포도의 액즙에 많이 함유되어 있다. Glucose is the central source of sugar metabolism in the body and is the most basic source of energy in the body. It is found in vegetables and fruits, and especially in the juice of grapes.
과당(fructose)은 과일과 꿀속에 존재하며 당 가운데 단맛이 가장 강하고 설탕과 전화당의 구성단위이다. 갈락토우즈(galactose)는 포도당과 결합하여 유당(lactose)이라 불리는 이당류의 형태로 존재하며 포도당보다 단맛은 약하고 물에 녹기 어렵다. Fructose is present in fruits and honey, the sweetest of sugars, and the building block of sugar and invert sugar. Galactose, combined with glucose, exists in the form of a disaccharide called lactose, which is less sweet than glucose and insoluble in water.
이당류(disacharide)는 설탕(sucrose), 맥아당(maltose), 유당(lactose) 등이 있다. 설탕(sucrose)은 포도당과 과당이 결합한 당이며 사탕수수, 사탕무 중에 많이 함유되어 있다. 젖당, 유당, 락토우즈(lactose)는 동물의 젖 속에 많으며 단맛을 적으며 유당은 물에 잘 녹지 않고 소화도 느리다. Disaccharides include sugar (sucrose), maltose (lactose) and lactose (lactose). Sugar (sucrose) is a combination of glucose and fructose and is found in sugar cane and sugar beet. Lactose, lactose and lactose are found in the milk of animals and are less sweet. Lactose is less soluble in water and slow to digest.
올리고당은 다당류이며, 당단백질이나 당지질의 구성성분으로서 세포내에서 주로 생체막에 부착되어 있고, 소포체와 골지체 등의 분비형 단백질과 결합되어 있으며, 갈락토올리고당, 이소말토올리고당 및 프락토올리고당이 있다. 복합탄수화물로 불리우는 다당류는 녹말, 글리코겐, 식이섬유소로 구분된다.Oligosaccharides are polysaccharides, constituents of glycoproteins or glycolipids, mainly attached to biological membranes within cells, bound to secretory proteins such as endoplasmic reticulum and Golgi, and include galactooligosaccharides, isomaltoligosaccharides, and fructooligosaccharides. Polysaccharides, called complex carbohydrates, are divided into starch, glycogen, and dietary fiber.
헥소산(hesoxan)은 전분, 녹말(starch)이고, 글리코겐(glycogen)은 동물의 저장용 탄수화물로 근육조직과 간에 저장한다. 섬유소(cellulose)는 다당류이다. Hexoxan is starch and starch. Glycogen is an animal carbohydrate that is stored in muscle tissue and liver. Cellulose is a polysaccharide.
본 발명에서 사용된 코팅층(50) 성분은 아마씨를 80∼120℃에서 10∼40분 동안 가열하여 아마씨에서 수분을 제거한 후 급속 냉각시키고, 급속 냉각된 아마씨를 착유기에 넣고 착유기의 온도 80∼120℃ 상태에서 착유한 후 200 메쉬에서 여과하여 여과된 아마씨유를 수득하고, 상기 여과된 아마씨유를 10∼20일 자연침전시킨 후 상층부를 회수하는 공정을 거쳐 정제된 아마씨유를 수득하여 제조된 악취 및 시안아이드(-CN) 독성이 제거된 아마씨유를 사용하는 것이 바람직하다.The coating layer 50 used in the present invention is heated to flaxseed at 80 ~ 120 ℃ for 10 to 40 minutes to remove moisture from the flaxseed and then rapidly cooled, put the fast cooled flaxseed in the milking machine temperature 80 ~ 120 ℃ Malodor produced by filtering flaxseed oil by milking in a state of 200 mesh to obtain a filtered flaxseed oil, and then purifying flaxseed oil by natural sedimentation of the filtered flaxseed oil for 10 to 20 days to obtain an upper layer. It is preferable to use flaxseed oil which is free of cyanide (-CN) toxicity.
본 발명에서 사용된 코팅층(50) 성분은 녹차추출물, γ-토코페놀 및 로즈마리 중에서 선택된 어느 하나의 항산화제 5∼10 중량% 추가하는 것이 바람직하다.The coating layer 50 component used in the present invention is preferably added to 5 to 10% by weight of any antioxidant selected from green tea extract, γ-tocophenol and rosemary.
본 발명에서 사용된 코팅층(50) 성분은 상전이 유도 촉매를 1∼5 중량% 추가하는 것이 바람직하며, 상기 상전이 유도 촉매는 유채씨유, 카놀라유; 콩단백 레스틴, 계란 레스틴, 트윈, 모노그린, 폴리글리세린 및 에스테르 지방산으로 구성되는 그룹으로부터 선택된 1종 이상의 식용 유화제; 및 글리세릴 모노올레이트(glyceryl monooleate), 글리세릴 모노리놀레이트(glyceryl monolinoleate), 글리세릴 모노아라키도네이트(glyceryl monoarachidonate) 및 글리세릴 모노스테아레이트(glyceryl monostearate)로 구성되는 그룹으로부터 선택된 1종 이상의 모노글리세라이드(monoglycerides); 중에서 선택된 어느 하나인 것이 바람직하다.The coating layer 50 component used in the present invention preferably adds 1 to 5% by weight of a phase change induction catalyst, and the phase transition catalyst includes rapeseed oil, canola oil; At least one edible emulsifier selected from the group consisting of soy protein restin, egg restin, tween, monogreen, polyglycerin and ester fatty acids; And one or more selected from the group consisting of glyceryl monooleate, glyceryl monolinoleate, glyceryl monoarachidonate and glyceryl monostearate. Monoglycerides; It is preferable that it is any one selected from among.
본 발명의 코팅층(50)은 당, 전분, 상전이 코팅제 및 상전이 유도 촉매를 혼합하여 3000rpm으로 고속회전하면서 상전이 코팅되는 것을 사용하는 것이 바람직하다.As the coating layer 50 of the present invention, it is preferable to use a sugar, starch, a phase change coating agent and a phase change induction catalyst to mix phase change while rotating at high speed at 3000 rpm.
본 발명의 코팅층(50)은 당, 전분, 상전이 코팅제 및 상전이 유도 촉매를 적당량 혼합하여 혼합물의 조성성분과 함유량을 조절하고 코팅층의 두께를 조절하여 외부충격에 깨지는 코팅층을 제조할 수 있다. The coating layer 50 of the present invention may be prepared by mixing an appropriate amount of sugar, starch, phase change coating agent and phase transition induction catalyst to control the composition and content of the mixture and to adjust the thickness of the coating layer to produce a coating layer that is broken by external impact.
<실시예 1> 상전이 코팅제인 상전이 수용성 아마씨유의 제조Example 1 Preparation of Phase Transition Water Soluble Flaxseed Oil as Phase Change Coating
아마씨를 100℃에서 20분 동안 가열하여 아마씨에서 수분을 제거한 후 급속 냉각시키고, 급속 냉각된 아마씨를 착유기에 넣고 착유기의 온도 100℃ 상태에서 착유한 후 200 메쉬에서 여과하여 여과된 아마씨유를 수득하고, 상기 여과된 아마씨유를 20일 자연침전시킨 후 상층부를 회수하는 공정을 거쳐 정제된 아마씨유를 수득하여 제조된 악취 및 시안아이드(-CN) 독성이 제거된 아마씨유를 제조하였다.The linseed was heated at 100 ° C. for 20 minutes to remove moisture from the flaxseed and then rapidly cooled. The fast-cooled flaxseed was placed in an milking machine and milked at a milking machine temperature of 100 ° C. and filtered at 200 mesh to obtain filtered linseed oil. After 20 days of natural sedimentation of the filtered flaxseed oil to obtain a purified flaxseed oil through a process of recovering the upper portion to prepare the flaxseed oil from which the odor and cyanide (-CN) toxicity was removed.
<실시예 2> 상전이 코팅제인 상전이 수용성 아마씨유의 제조Example 2 Preparation of Phase Transition Water Soluble Flaxseed Oil as Phase Change Coating
실시예 1에서 제조된 아마씨유 50g과 토코페놀 20g을 물 25g에 혼합한 후 유채씨유 5g을 가하여 3000rpm으로 고속회전하면서 코팅하여 상전이 코팅제인 상전이 수용성 아마씨유 조성물을 제조하였다. 50 g of flaxseed oil prepared in Example 1 and 20 g of tocophenol were mixed in 25 g of water, and then 5 g of rapeseed oil was added thereto and coated with high speed rotation at 3000 rpm to prepare a phase change water-soluble flaxseed oil composition as a phase change coating agent.
<실시예 3> 상전이 코팅제인 상전이 수용성 아마씨유의 제조Example 3 Preparation of Phase Transition Water Soluble Flaxseed Oil as Phase Change Coating
실시예 1에서 제조된 아마씨유 50g과 토코페놀 20g을 물 25g에 혼합한 후 카놀라유 5g을 가하여 3000rpm으로 고속회전하면서 코팅하여 상전이 코팅제인 상전이 수용성 아마씨유 조성물을 제조하였다. 50 g of flaxseed oil prepared in Example 1 and 20 g of tocophenol were mixed in 25 g of water, and 5 g of canola oil was added thereto, followed by coating at high speed at 3000 rpm to prepare a phase-transfer water-soluble flaxseed oil composition as a phase change coating agent.
<실시예 4> 상전이 코팅제인 상전이 수용성 아마씨유의 제조Example 4 Preparation of Phase Transition Water Soluble Flaxseed Oil as Phase Change Coating
실시예 1에서 제조된 아마씨유 50g과 토코페놀 20g을 물 25g에 혼합한 후 글리세릴 모노 올레이트 5g을 가하여 3000rpm으로 고속회전하면서 코팅하여 상전이 코팅제인 상전이 수용성 아마씨유 조성물을 제조하였다. 50 g of flaxseed oil prepared in Example 1 and 20 g of tocophenol were mixed in 25 g of water, and 5 g of glyceryl monooleate was added thereto, followed by coating at high speed at 3000 rpm to prepare a phase change water-soluble linseed oil composition as a phase change coating agent.
<실시예 5> 상전이 코팅제인 상전이 수용성 아마씨유의 제조Example 5 Preparation of Phase Transition Water Soluble Flaxseed Oil as Phase Change Coating
실시예 1에서 제조된 아마씨유 50g과 토코페놀 20g을 물 25g에 혼합한 후 상전이유도 촉매(트웬 60 : Tween 60) 5g을 가하여 3000rpm으로 고속회전하면서 코팅하여 상전이 코팅제인 상전이 수용성 아마씨유 조성물을 제조하였다. 50 g of flaxseed oil prepared in Example 1 and 20 g of tocophenol were mixed in 25 g of water, and then, 5 g of a phase transfer induction catalyst (Twen 60: Tween 60) was added thereto, followed by coating at high speed at 3000 rpm to prepare a phase change water-soluble flaxseed oil composition as a phase change coating agent. It was.
<실시예 6> 외부충격에 깨지는 코팅층의 제조Example 6 Preparation of a Coating Layer Cracked in External Shock
엿당 50 중량%, 상전이 코팅제 45 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 -10℃에서 선택적 용해되고, 엿당 50 중량%, 전분 5 중량%, 상전이 코팅제 40 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 0℃에서 선택적 용해되며, 엿당 50 중량%, 전분 10 중량%, 상전이 코팅제 35 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 5℃에서 선택적 용해되고, 엿당 50 중량%, 전분 20 중량%, 상전이 코팅제 25 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 10℃에서 선택적 용해되며, 엿당 40 중량%, 전분 20 중량%, 상전이 코팅제 35 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 15℃에서 선택적 용해되고, 엿당 50 중량%, 전분 20 중량%, 상전이 코팅제 25 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 20℃에서 선택적 용해되며, 엿당 50 중량%, 전분 25 중량%, 상전이 코팅제 20 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 25℃에서 선택적 용해되고, 엿당 50 중량%, 전분 30 중량%, 상전이 코팅제 25 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 30℃에서 선택적 용해되고, 엿당 40 중량%, 전분 40 중량%, 상전이 코팅제 15 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 35℃에서 선택적 용해되고, 엿당 30 중량%, 전분 50 중량%, 상전이 코팅제 15 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 40℃에서 선택적 용해된다. 상기 코팅층은 외부충격에 의하여 깨진다.The coating layer consisting of 50% by weight of the malt, 45% by weight of the phase change coating agent, and 5% by weight of the phase induction catalyst is selectively dissolved at -10 ° C, and 50% by weight, starch 5% by weight, 40% by weight of the phase change coating agent, and phase induction catalyst The coating layer consisting of 5% by weight is selectively dissolved at 0 ° C, and the coating layer consisting of 50% by weight of starch, 10% by weight of starch, 35% by weight of phase change coating agent, and 5% by weight of phase induction catalyst is selectively dissolved at 5 ° C, and 50% by weight The coating layer consisting of 20% by weight, 20% by weight starch, 25% by weight phase transition coating, and 5% by weight phase induction catalyst is selectively dissolved at 10 ° C., 40% by weight, 20% by weight starch, 35% by weight phase change coating, and phase transition. The coating layer consisting of 5% by weight of the induction catalyst is selectively dissolved at 15 ° C, and the coating layer consisting of 50% by weight of starch, 20% by weight of starch, 25% by weight of phase change coating agent, and 5% by weight of phase induction catalyst is selectively dissolved at 20 ° C. 50 wt% per starch, 25 wt% starch, 20 wt% phase change coating agent, and 5 wt% phase transition induction catalyst were selectively dissolved at 25 ° C., 50 wt% per starch, 30 wt% starch, phase change coating 25 The coating layer consisting of the weight percent, and 5% by weight of the phase induction catalyst is selectively dissolved at 30 ° C, and the coating layer of 40% by weight, starch 40% by weight, 15% by weight of the phase change coating agent, and 5% by weight of the phase induction catalyst is 35 ° C. Is selectively dissolved at 30% by weight, 50% by weight starch, 15% by weight phase change coating agent, and 5% by weight phase induction catalyst are selectively dissolved at 40 ° C. The coating layer is broken by external impact.
<실시예 7> 외부충격에 깨지는 코팅층의 제조Example 7 Preparation of a Coating Layer Cracked in External Shock
포도당 50 중량%, 상전이 코팅제 45 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 -10℃에서 선택적 용해되고, 포도당 50 중량%, 전분 5 중량%, 상전이 코팅제 40 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 0℃에서 선택적 용해되며, 포도당 50 중량%, 전분 10 중량%, 상전이 코팅제 35 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 5℃에서 선택적 용해되고, 포도당 50 중량%, 전분 20 중량%, 상전이 코팅제 25 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 10℃에서 선택적 용해되며, 포도당 40 중량%, 전분 20 중량%, 상전이 코팅제 35 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 15℃에서 선택적 용해되고, 포도당 50 중량%, 전분 20 중량%, 상전이 코팅제 25 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 20℃에서 선택적 용해되며, 포도당 50 중량%, 전분 25 중량%, 상전이 코팅제 20 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 25℃에서 선택적 용해되고, 포도당 50 중량%, 전분 30 중량%, 상전이 코팅제 25 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 30℃에서 선택적 용해되고, 포도당 40 중량%, 전분 40 중량%, 상전이 코팅제 15 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 35℃에서 선택적 용해되고, 포도당 30 중량%, 전분 50 중량%, 상전이 코팅제 15 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 40℃에서 선택적 용해된다. 상기 코팅층은 외부충격에 의하여 깨진다.The coating layer consisting of 50% by weight glucose, 45% by weight phase change coating agent, and 5% by weight phase induction catalyst was selectively dissolved at −10 ° C., 50% by weight glucose, 5% by weight starch, 40% by weight phase change coating agent, and phase induction catalyst. The coating layer consisting of 5% by weight is selectively dissolved at 0 ° C, and the coating layer consisting of 50% by weight of glucose, 10% by weight of starch, 35% by weight of phase change coating agent, and 5% by weight of phase induction catalyst is selectively dissolved at 5 ° C. The coating layer consisting of 20% by weight, 20% by weight starch, 25% by weight phase transition coating, and 5% by weight phase induction catalyst is selectively dissolved at 10 ° C., 40% by weight glucose, 20% by weight starch, 35% by weight phase change coating, and phase transition. The coating layer consisting of 5% by weight of the induction catalyst is selectively dissolved at 15 ° C, and the coating layer consisting of 50% by weight of glucose, 20% by weight of starch, 25% by weight of phase change coating agent, and 5% by weight of phase induction catalyst is 20 ° C. It is selectively dissolved in the coating, the coating layer consisting of 50% by weight of glucose, 25% by weight starch, 20% by weight phase change coating agent, and 5% by weight phase transition induction catalyst is selectively dissolved at 25 ℃, 50% by weight of glucose, 30% by weight starch, phase transition The coating layer consisting of 25% by weight of the coating agent, and 5% by weight of the phase induction catalyst is selectively dissolved at 30 ° C., and the coating layer of 40% by weight of glucose, 40% by weight of starch, 15% by weight of the phase change coating agent, and 5% by weight of the phase induction catalyst is The coating layer is selectively dissolved at 35 ° C., and the coating layer consisting of 30% by weight of glucose, 50% by weight of starch, 15% by weight of phase change coating agent, and 5% by weight of phase induction catalyst is selectively dissolved at 40 ° C. The coating layer is broken by external impact.
<실시예 8> 외부충격에 깨지는 코팅층의 제조Example 8 Preparation of a Coating Layer Cracked in External Shock
맥아당 50 중량%, 상전이 코팅제 45 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 -10℃에서 선택적 용해되고, 맥아당 50 중량%, 전분 5 중량%, 상전이 코팅제 40 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 0℃에서 선택적 용해되며, 맥아당 50 중량%, 전분 10 중량%, 상전이 코팅제 35 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 5℃에서 선택적 용해되고, 맥아당 50 중량%, 전분 20 중량%, 상전이 코팅제 25 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 10℃에서 선택적 용해되며, 맥아당 40 중량%, 전분 20 중량%, 상전이 코팅제 35 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 15℃에서 선택적 용해되고, 맥아당 50 중량%, 전분 20 중량%, 상전이 코팅제 25 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 20℃에서 선택적 용해되며, 맥아당 50 중량%, 전분 25 중량%, 상전이 코팅제 20 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 25℃에서 선택적 용해되고, 맥아당 50 중량%, 전분 30 중량%, 상전이 코팅제 25 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 30℃에서 선택적 용해되고, 맥아당 40 중량%, 전분 40 중량%, 상전이 코팅제 15 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 35℃에서 선택적 용해되고, 맥아당 30 중량%, 전분 50 중량%, 상전이 코팅제 15 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 40℃에서 선택적 용해된다. 상기 코팅층은 외부충격에 의하여 깨진다.The coating layer consisting of 50% by weight of malt sugar, 45% by weight of phase transition coating, and 5% by weight of phase induction catalyst is selectively dissolved at -10 ° C, and 50% by weight of malt, 5% by weight of starch, 40% by weight of phase change coating, and phase induction catalyst. The coating layer consisting of 5% by weight is selectively dissolved at 0 ° C, and the coating layer consisting of 50% by weight of malt sugar, 10% by weight of starch, 35% by weight of phase change coating agent, and 5% by weight of phase induction catalyst is selectively dissolved at 5 ° C and 50% by weight of malt. The coating layer consisting of 20% by weight, 20% by weight starch, 25% by weight phase transition coating, and 5% by weight phase induction catalyst is selectively dissolved at 10 ° C., 40% by weight, 20% by weight starch, 35% by weight phase change coating, and phase transition. The coating layer consisting of 5% by weight of the induction catalyst is selectively dissolved at 15 ° C, and the coating layer consisting of 50% by weight of malt sugar, 20% by weight of starch, 25% by weight of phase change coating agent, and 5% by weight of phase induction catalyst is 20 ° C. Is selectively dissolved at 50 ° C., malt 50% by weight, starch 25% by weight, phase change coating 20% by weight, and phase change induction catalyst 5% by weight. The coating layer consisting of 25% by weight of the coating agent, and 5% by weight of the phase induction catalyst is selectively dissolved at 30 ° C., and the coating layer of 40% by weight of malt, starch 40% by weight, 15% by weight of the phase change coating agent, and 5% by weight of the phase induction catalyst is The coating layer is selectively dissolved at 35 ° C., and the coating layer consisting of 30% by weight of malt sugar, 50% by weight starch, 15% by weight phase transfer coating agent, and 5% by weight phase induction catalyst is selectively dissolved at 40 ° C. The coating layer is broken by external impact.
<실시예 9> 외부충격 변화에 따른 코팅층의 깨지는 상태 변화<Example 9> Change in the cracking state of the coating layer according to the external impact change
실시예 8에서 제조된 맥아당 50 중량%, 상전이 코팅제 45 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 -10℃에서 0 내지 48시간 동안 깨지지 않았다. 또한 실시예 8에서 제조된 맥아당 50 중량%, 전분 5 중량%, 상전이 코팅제 40 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 0℃에서 0 내지 48시간 동안 깨지지 않았다.The coating layer composed of 50% by weight malt sugar, 45% by weight phase change coating agent, and 5% by weight phase induction catalyst prepared in Example 8 was not broken at -10 ° C for 0 to 48 hours. In addition, the coating layer composed of 50% by weight of malt sugar, 5% by weight starch, 40% by weight phase change coating agent, and 5% by weight phase induction catalyst prepared in Example 8 did not break at 0 ° C for 0 to 48 hours.
실시예 8에서 제조된 맥아당 50 중량%, 전분 10 중량%, 상전이 코팅제 35 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 5℃에서 0 내지 48시간 동안 깨지지 않았다. 또한 실시예 8에서 제조된 맥아당 50 중량%, 전분 20 중량%, 상전이 코팅제 25 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 10℃에서 0 내지 48시간 동안 깨지지 않았다.The coating layer composed of 50% by weight malt, 10% by weight starch, 35% by weight phase change coating agent, and 5% by weight phase induction catalyst prepared in Example 8 was not broken at 5 ° C. for 0 to 48 hours. In addition, the coating layer made of 50% by weight malt, starch 20% by weight, 25% by weight phase change coating agent, and 5% by weight phase induction catalyst prepared in Example 8 did not break at 10 ℃ for 0 to 48 hours.
실시예 8에서 제조된 포도당 40 중량%, 전분 20 중량%, 상전이 코팅제 35 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 15℃에서 0 내지 48시간 동안 깨지지 않았다. 또한 실시예 8에서 제조된 맥아당 50 중량%, 전분 20 중량%, 상전이 코팅제 25 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 20℃에서 0 내지 48시간 동안 깨지지 않았다. 또한 실시예 8에서 제조된 맥아당 50 중량%, 전분 30 중량%, 상전이 코팅제 25 중량%, 및 상전이유도 촉매 5 중량%로 이루어진 코팅층은 30℃에서 0 내지 48시간 동안 깨지지 않았다. The coating layer composed of 40% by weight of glucose, 20% by weight of starch, 35% by weight of phase change coating agent, and 5% by weight of phase induction catalyst prepared in Example 8 was not broken at 15 ° C. for 0 to 48 hours. In addition, the coating layer made of 50% by weight malt, starch 20% by weight, 25% by weight phase change coating agent, and 5% by weight phase induction catalyst prepared in Example 8 did not break at 20 ℃ 0 to 48 hours. In addition, the coating layer composed of 50% by weight malt, starch 30% by weight, 25% by weight phase change coating agent, and 5% by weight phase induction catalyst prepared in Example 8 did not break at 30 ℃ for 0 to 48 hours.
본 발명에서 유산균 액체(32)는 증류수가 함유된 순수한 유산균 액체뿐만 아니라 증류수가 함유된 유산균 액체에 배지 분말 또는 배지 액체가 혼합된 것이 바람직하다.In the present invention, the lactic acid bacteria liquid 32 is preferably mixed with the medium powder or the medium liquid in the lactic acid bacteria liquid containing distilled water as well as the pure lactic acid bacteria liquid containing distilled water.
본 발명에서 유산균 분말(30) 또는 유산균 액체(32)는 배지와 혼합된 것이 바람직하다. 본 발명에서 유산균은 부패 식중독균과 성장패턴이 유사하고 성장시 유기산을 형성하여 pH를 떨어뜨리고, 인체 및 환경에 해가 없는 미생물인 것이 바람직하다. 본 발명에서 부패 식중독균은 대장균 O-157 또는 살모넬라인 것이 바람직하다.In the present invention, the lactic acid bacteria powder 30 or the lactic acid bacteria liquid 32 is preferably mixed with the medium. In the present invention, lactic acid bacteria are similar to the rotted food poisoning bacteria and growth patterns, and form organic acids during growth to drop the pH, it is preferable that the microorganisms are harmless to the human body and the environment. In the present invention, the decaying food poisoning bacteria E. coli O-157 or Salmonellane is preferred.
본 발명에서 저장물질의 부패 체크용 유산균은 유산균뿐만 아니라 효모, 곰팡이로 이루어진 군으로부터 선택되는 것이 바람직하며, 락토바실러스 에시도필러스(Lactobacillus acidophilus)인 것이 바람직하다.In the present invention, the lactic acid bacteria for decay check of the storage material is preferably selected from the group consisting of not only lactic acid bacteria, but also yeast, mold, and preferably Lactobacillus acidophilus.
본 발명에서 배지는 유산균의 성장과 동시에 발생하는 유기산에 의해서 배지의 pH가 낮아지는 조성인 것이 바람직하며, MRS, MRS 배지가 25%로 희석된 배지, 최소배지 및 포도당 100 g/ℓ를 포함하는 최소배지로 이루어진 군으로부터 선택되는 어느 하나인 것이 바람직하고, MRS 배지가 25%로 희석된 배지인 것이 바람직하다.In the present invention, the medium is preferably a composition in which the pH of the medium is lowered by the organic acid generated at the same time as the growth of lactic acid bacteria, MRS, medium containing 25% diluted MRS medium, the minimum medium and glucose 100 g / L It is preferable that it is any one selected from the group which consists of a minimum medium, and it is preferable that MRS medium is a medium diluted to 25%.
본 발명에서 유산균 분말은 유산균 분말 및 배지 분말의 혼합분말이 바람직하며, 동결건조된 유산균 및 배지 분말을 0.01 ~ 0.1 : 99.99 ~ 99.9의 비로 혼합하여 사용하는 것이 바람직하며, 동결건조된 유산균 및 배지 분말을 0.05 ~ 0.1 : 99.95 ~ 99.9의 비율로 혼합하여 사용하는 것이 바람직하고, 동결건조된 유산균 및 배지 분말을 0.1:99.9의 비율로 혼합하여 사용하는 것이 바람직하며, 추가로 곰팡이 성장 저해제를 포함하는 것이 바람직하다. 본 발명에서 곰팡이 성장 저해제는 항진균성 폴리엔(polyene antifungals), 니스타틴 (nystatin), 암포테리신 B(amphotericin B), 피마리신(pimaricin)으로 이루어진 군으로부터 선택되는 것이 바람직하다.In the present invention, the lactic acid bacteria powder is preferably a mixed powder of lactic acid bacteria powder and medium powder, it is preferable to use a mixture of lyophilized lactic acid bacteria and medium powder in a ratio of 0.01 ~ 0.1: 99.99 ~ 99.9, lyophilized lactic acid bacteria and medium powder It is preferable to use the mixture in the ratio of 0.05 ~ 0.1: 99.95 ~ 99.9, it is preferable to use a mixture of lyophilized lactic acid bacteria and medium powder in the ratio of 0.1: 99.9, and further include a fungal growth inhibitor desirable. In the present invention, the fungal growth inhibitor is preferably selected from the group consisting of antifungal polyenes (polyene antifungals), nystatin, amphotericin B (amphotericin B), pimaricin (pimaricin).
본 발명에서 유산균인 락토바실러스 에시도필러스 (Lactobacillus acidophilus)를 여러 배지에서 0 내지 21일간 배양한 결과, 성장과 동시에 젖산 등의 생성으로 pH가 낮아졌으며, 한번 낮아진 pH는 3주까지도 유지되는 것을 확인하고, 유산균을 pH 지시약, 금속비누 입자 및 pH 민감성 고분자입자 등의 pH 표지물질과 함께 배양한 결과, 유산균의 성장으로 pH가 떨어짐과 동시에 배지의 색이 변하여 산성임을 나타내었다. 이때, 금속비누 입자나 pH 민감성 고분자 입자의 경우 배지를 중성으로 조정하여도, 산성의 색을 유지하였다. 본 발명의 라벨을 냉동 보존할 경우 라벨이 효과적으로 사용되려면 냉동온도(예; -20℃)에서도 균이 살아 있다가 온도가 오를 경우 성장할 수 있어야 한다.As a result of incubating Lactobacillus acidophilus, which is a lactic acid bacterium, in various media for 0 to 21 days in the present invention, the pH was lowered due to the growth and production of lactic acid, and the pH was lowered up to 3 weeks. As a result, the lactic acid bacteria were incubated with pH indicators such as pH indicators, metal soap particles, and pH sensitive polymer particles. As a result, the pH of the lactic acid bacteria decreased and the color of the medium changed to indicate that the acid was acidic. At this time, in the case of metal soap particles or pH sensitive polymer particles, the acidic color was maintained even when the medium was adjusted to neutral. When the label of the present invention is cryopreserved, in order for the label to be effectively used, the bacterium should be able to grow even when the temperature is raised even at the freezing temperature (eg, -20 ° C).
본 발명에서 라벨을 저장공간에서 사용하는 중 온도가 빙점 이하로 내려가는 경우 라벨이 제대로 작동하기 위해서는 빙점 이하에서도 균이 생존해 있어야 한다. 무엇보다도 장기간 저온상태에서 저장하는 경우 유산균이 성장은 하지 않더라도 생존해 있어야 한다. 유산균이 냉동저장 후 배양하여도 냉동저장 없이 배양한 균 또는 냉장저장 후 배양한 균과 마찬가지로 배양이 가능하며 배양과 동시에 배양액의 pH를 떨어뜨린 것을 확인할 수 있다. 또한 본 발명에서 유산균을 동결건조한 후 접종농도를 달리하여 배양함으로써, 동결건조된 유산균은 0.1%(W/V) 이하를 사용하여야 다른 물질에 의한 탁도 등으로 색의 판단에 영향이 없고, CFU는 10,000 CFU/㎖ 이상이 되어야 균의 성장이 1-2일 내에 이루어진다.In the present invention, when the temperature falls below the freezing point while the label is used in the storage space, bacteria must survive even below the freezing point in order for the label to work properly. Above all, if stored at low temperatures for a long time, lactic acid bacteria must survive even if they do not grow. Even if the lactic acid bacteria were cultured after freezing storage, the cultures could be cultured like the microorganisms cultured without freezing storage or cultured after cold storage and the pH of the culture solution was dropped at the same time as the culture. In addition, by lyophilizing the lactic acid bacteria in the present invention by incubating at different concentrations, lyophilized lactic acid bacteria should be used less than 0.1% (W / V) has no effect on the determination of color due to turbidity by other materials, At least 10,000 CFU / ml, the growth of bacteria occurs within 1-2 days.
본 발명에서 동결건조된 유산균과 pH 지시약을 포함하도록 제조된 라벨을 다양한 온도에 노출한 후 미생물의 배양상태에 따른 pH 지시약의 색상을 비교함으로써 저장 물질의 부패판단을 수행할 수 있다. 본 발명의 라벨은 저장 온도변화의 누적에 따른 저장물질의 부패가능성 판단용 라벨로 유용하게 사용될 수 있다.In the present invention, after the label prepared to include the lyophilized lactic acid bacteria and the pH indicator at various temperatures, corruption of the storage material may be performed by comparing the color of the pH indicator according to the culture state of the microorganism. The label of the present invention can be usefully used as a label for determining the perishability of the storage material according to the accumulation of storage temperature change.
본 발명에서 유산균은 대표적인 부패 식중독균인 대장균 O-157, 살모넬라와 성장패턴이 유사하고 성장시 유기산을 형성하여 pH를 떨어뜨리고 인체 및 환경에 해가 없는 미생물은 무엇이든 사용가능하며, 바람직하게는 유산균, 효모, 곰팡이균 등이 있으며, 가장 바람직하게는 락토바실러스 에시도필러스 (Lactobacillus acidophilus)를 사용하는 것이 바람직하다.In the present invention, the lactic acid bacteria are similar to the growth pattern of Escherichia coli O-157, Salmonella, which is a decaying food poisoning bacterium. , Yeast, fungi, and the like, and most preferably Lactobacillus acidophilus is used.
본 발명에서 배지는 유산균의 성장과 동시에 발생하는 유기산에 의해서 배지의 pH가 낮아지도록 배지 조성을 결정하고, 바람직하게는 MRS, MRS 배지가 25%로 희석된 배지, 최소배지 및 포도당 100 g/ℓ를 포함하는 최소배지 등이 이용될 수 있으며, 더욱 바람직하게는 MRS 배지가 25%로 희석된 배지가 이용될 수 있다. In the present invention, the medium composition is determined so that the pH of the medium is lowered by the organic acid generated at the same time as the growth of the lactic acid bacteria, and preferably, the medium, the minimum medium and 100 g / L of the MRS and the MRS medium diluted to 25% Minimal medium including, etc. may be used, more preferably, a medium in which MRS medium is diluted to 25% may be used.
본 발명에서 유산균이 성장하지 않는 경우의 배지 pH는 중성이 되도록 하며 배지 내에 아미노산 성분이 많을 경우 유산균에 의해 분해되면서 발생하는 암모니아로 pH가 오르기 때문에 이를 하향 조절한다. 유산균이 성장 정도와 비례하여 유기산을 생성하게 되고, 유기산은 배지의 pH를 떨어뜨려 산성이 된다. pH가 낮아지면 색이 변화되는 지시약을 배지에 첨가해 놓으면 저장물질과 같은 온도변화를 받은 유산균이 성장하면서 색이 변하게 되고 이것은 저장물질 내에 부패균이 있다면 성장할 수 있다.In the present invention, when the lactic acid bacteria do not grow, the pH of the medium is neutral, and when there are many amino acid components in the medium, the pH is increased by ammonia generated while being degraded by lactic acid bacteria. The lactic acid bacteria produce an organic acid in proportion to the degree of growth, and the organic acid becomes acidic by dropping the pH of the medium. When the pH is lowered, a color change indicator is added to the medium, and the color changes as the lactic acid bacteria are changed to the same temperature as the storage material, which can grow if there is a decay bacteria in the storage material.
본 발명에서 유산균 혼합분말은 동결건조된 유산균 및 배지 분말을 0.01 ~ 0.1 : 99.9 ~ 99.99의 비율, 바람직하게는 0.05 ~ 0.1 : 99.9 ~ 99.95의 비율, 가장 바람직하게는 0.1:99.9의 비율로 혼합하여 사용하는 것이 바람직하다.In the present invention, the lactic acid bacteria mixed powder is mixed with the lyophilized lactic acid bacteria and the medium powder at a ratio of 0.01 to 0.1: 99.9 to 99.99, preferably at a ratio of 0.05 to 0.1: 99.9 to 99.95, and most preferably at a ratio of 0.1: 99.9. It is preferable to use.
본 발명의 라벨 제조시 무균상황을 유지할 필요를 없게 하기 위해서 유산균은 동결건조된 분말형태로, 배지 분말과 같이 미리 혼합하고 물과는 분리된 상태로 제작한다. 이 경우 잡균의 배양을 억제할 수 있으나 무균상황이 아니어서 곰팡이가 혼입되어 유산균 배양시 곰팡이가 성장하면서 유기산을 분해하고, pH가 증가하여In order to avoid the need to maintain a sterile state in the manufacture of the label of the present invention lactic acid bacteria in the form of lyophilized powder, pre-mixed with the medium powder and prepared in a state separated from water. In this case, the culture of various bacteria can be suppressed, but it is not a sterile situation, and the fungus is mixed.
색이 변하는 것을 방지키 위해서 본 발명의 혼합분말에 곰팡이 성장 저해제를 추가로 첨가할 수 있고, 곰팡이 성장 저해제로는 항진균성 폴리엔 (polyene antifungals), 니스타틴(nystatin), 암포테리신 B(amphotericin B), 피마리신(pimaricin) 등이 사용될 수 있다.In order to prevent color change, a fungal growth inhibitor may be additionally added to the mixed powder of the present invention, and as a fungal growth inhibitor, antifungal polyene (polyene antifungals), nystatin, and amphotericin B (amphotericin) B), pimaricin and the like can be used.
본 발명에서 pH 지시약은 중성과 산성에서 색의 변화가 뚜렷한 지시약은 모두 사용가능하며, 바람직하게는 메틸레드, 메틸블루, 브로모크레솔 그린-메틸 레드(Bromocresol Green-Methyl Red), 브로모크레솔 그린(Bromocresol Green), 브레모페놀 블루(Bromophenol Blue) 등을 사용 가능하다.In the present invention, the pH indicator may be used as the indicator of the color change in neutral and acidic, preferably methyl red, methyl blue, bromocresol Green-Methyl Red, bromocre Bromocresol Green and Bremophenol Blue can be used.
본 발명에서 유산균 성장으로 발생한 유기산으로 배양액의 pH가 낮아졌더라도 다른 오염균의 성장 등의 이유로 pH가 다시 상승하는 경우 pH가 중성으로 될 수 있으므로 pH가 오를 경우에도 색이 변해서는 안 된다. 한 번 낮은 pH에 의해 색이 변하면 그 색이 유지되도록 하는 방법이 필요하다. In the present invention, even when the pH of the culture medium is lowered due to the growth of lactic acid bacteria, the pH may be neutral when the pH rises again due to growth of other contaminants, and thus the color should not change even when the pH rises. Once a color changes due to a low pH, a method is needed to maintain that color.
본 발명에서 pH 지시약 액체(22)는 증류수가 함유된 pH 지시약 액체가 바람직하다. 본 발명에서 사용되는 pH 표지물질로서 pH 지시약은 일반적인 pH 지시약뿐만 아니라 금속비누 입자 및 pH 민감성 고분자입자 등이 이용될 수 있다. In the present invention, the pH indicator liquid 22 is preferably a pH indicator liquid containing distilled water. As the pH indicator used in the present invention, the pH indicator may be used as the general pH indicator as well as metal soap particles and pH sensitive polymer particles.
본 발명에서 일반적인 pH 지시약은 중성과 산성에서 색의 변화가 뚜렷한 것 사용하는 것이 바람직하며, 메틸레드 및 메틸블루의 혼합액인 것이 바람직하다. 본 발명에서 pH 지시약은 중성과 산성에서 색의 변화가 뚜렷한 지시약은 모두 사용가능하며, 바람직하게는 메틸레드, 메틸블루, 브로모크레솔 그린-메틸레드 (Bromocresol Green-Methyl Red), 브로모크레솔 그린(Bromocresol Green), 브레모페놀 블루(Bromophenol Blue) 등을 사용 가능하다. 본 발명에서 pH 지시약 액체(22)는 증류수가 함유된 pH 지시약 액체가 바람직하다. In the present invention, it is preferable to use a general pH indicator having a clear change in color in neutrality and acidity, and a mixture of methyl red and methyl blue. In the present invention, the pH indicator may be used as the indicator of the color change in neutral and acidic, preferably methyl red, methyl blue, bromocresol Green-Methyl Red, bromocre Bromocresol Green and Bremophenol Blue can be used. In the present invention, the pH indicator liquid 22 is preferably a pH indicator liquid containing distilled water.
본 발명에서 pH 지시약은 메틸레드(methyl red) 0.1g, 메틸렌블루 (methylene blue) 0.1 g을 에탄올 190 ㎖에 용해하고 물을 채워 200㎖로 만든다. 중성에서 산성으로 변할 경우 색이 변하는 지시약은 모두 적용될 수 있다.In the present invention, the pH indicator is prepared by dissolving 0.1 g of methyl red and 0.1 g of methylene blue in 190 ml of ethanol and filling it with water to make 200 ml. Any color-changing indicator can be applied when changing from neutral to acidic.
본 발명에서 pH 지시약은 금속비누 입자 또는 pH 민감성 고분자입자인 것이 바람직하며, 금속비누 입자는 안료와 올레인산을 2가 이온과 비누화 반응을 시켜서 금속비누 알갱이 형태로 만든 것이 바람직하다.In the present invention, the pH indicator is preferably metal soap particles or pH-sensitive polymer particles, and the metal soap particles are preferably in the form of metal soap granules by a saponification reaction of a pigment and oleic acid with divalent ions.
본 발명에서 금속비누 입자는 안료와 올레인산 등을 칼슘 등의 2가 이온과 비누화 반응을 시켜서 금속비누 알갱이 형태를 만든 것으로 유기산으로 pH가 낮아지면 금속비누는 다시 분해되어 함유되어 있던 염료가 분산되면서 라벨이 전체적으로 안료의 색을 띠게 된다. 이 반응은 pH가 라벨 내의 성분이 시간이 지남에 따라 pH가 중성으로 변해도 자발적으로 염료가 다시 금속비누의 알갱이가 되지 않으므로 라벨 내의 색을 유지할 수 있다.In the present invention, the metal soap particles are made of granulated metal soap by reacting pigments and oleic acid with divalent ions such as calcium, and when the pH is lowered with organic acids, the metal soap is decomposed again to disperse the dye. This becomes the color of the pigment as a whole. This reaction maintains the color in the label as the dye does not spontaneously repel the metal soap even if the pH changes to neutral over time as the components in the label change over time.
본 발명에서 금속비누는 안료 및 올레인산을 칼슘 2가 이온과 비누화 반응을 시켜서 금속비누 알갱이 형태를 제조한다. D&C Red No.6 Barium-lake 10㎎, 올레인산 15g, Ca(OH)2 2.204g, H2O 5.67g 및 리파아제 0.0375g을 60℃에서 480 rpm으로 2.5 시간 동안 반응한 후 잘게 분쇄하여 사용한다.In the present invention, the metal soap is a saponification reaction of the pigment and oleic acid with calcium divalent ions to produce a metal soap granule form. 10 mg of D & C Red No. 6 Barium-lake, 15 g of oleic acid, 2.204 g of Ca (OH) 2, 5.67 g of H 2 O and 0.0375 g of lipase were reacted at 60 ° C. at 480 rpm for 2.5 hours, and then finely ground.
본 발명에서 pH 지시약은 pH 민감성 고분자가 바람직하며, 키토산, 폴리라이신(polylysine), 폴리에틸렌이민(polyethyleneimine; PEI), 디에틸아미노에틸-덱스트란(diethylaminoethyl-dextran; DEAE-dextran) 및 폴리(아마이도아민) 덴드리머[poly(amidoamine) (PAMAM) dendrimers]로 이루어진 군으로부터 선택되는 것이 바람직하다.In the present invention, the pH indicator is preferably a pH-sensitive polymer, chitosan, polylysine, polyethyleneimine (PEI), diethylaminoethyl-dextran (DEAE-dextran) and poly (amido) Amine) dendrimers [poly (amidoamine) (PAMAM) dendrimers] is preferably selected from the group consisting of.
본 발명에서 pH 민감성 고분자 입자는 안료를 낮은 pH에서 고분자가 팽창되는 염기성 고분자로 포집하고 있는 형태로 제조되고, 유기산으로 pH가 낮아지면 고분자입자 안에 포집된 안료가 배지로 방출되어 안료의 색을 띠게 하고 다시 중성이 되도 안료가 수축된 고분자입자 내로 들어오지 않기 때문에 안료의 색을 유지할 수 있다. 본 발명에서 염기성 고분자에는 키토산, 폴리라이신(polylysine), 폴리에틸렌이민 (polyethyleneimine; PEI), 디에틸아미노에틸-덱스트란(diethylaminoethyl-dextran; DEAE-dextran), 폴리(아마이도아민) 덴드리머 [poly(amidoamine) (PAMAM) dendrimers] 등이 이용될 수 있다. 본 발명에서 안료는 D&C Red No.6 Barium-lake, 붉은 색 유기염료, 무기염료 등이 이용될 수 있다.In the present invention, the pH-sensitive polymer particles are prepared in a form in which the pigment is collected as a basic polymer in which the polymer is expanded at low pH, and when the pH is lowered with the organic acid, the pigment trapped in the polymer particles is released into the medium to have a color of the pigment. Even if it is neutral again, the color of the pigment can be maintained since the pigment does not enter the shrinked polymer particles. In the present invention, the basic polymer includes chitosan, polylysine, polyethyleneimine (PEI), diethylaminoethyl-dextran (DEAE-dextran), and poly (amidoamine) dendrimer [poly (amidoamine). (PAMAM) dendrimers] and the like can be used. As the pigment in the present invention, D & C Red No. 6 Barium-lake, a red organic dye, an inorganic dye, and the like may be used.
본 발명에서 pH 민감성 고분자는 많은 종류가 있고 낮은 pH에서 팽창하는 염기성 고분자는 모두 적용될 수 있다. 0.5g의 키토산을 0.1%(V/V)의 초산 50㎖에 용해시키고 여기에 키토산의 3-80%(Wt%)까지 EDTA를 추가하여 강도를 높일 수 있다. 여기에 안료(D&C Red No.6 Barium-lake; Warner-Jenkinson, USA)를 1-5㎎ 첨가하고 5M NaOH로 pH를 중화한다. 여기에 EDAC [1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride; Sigma, USA]를 10-100mM 되도록 첨가한다. 상온에서 4시간 동안 교반한 후 증류수로 2회 세척 0.5 M NaCl, 0.025M NaOH로 2회 세척 및 0.05M NaOH로 2회 세척 후 증류수로 1회 세척하여 냉장보관한다.There are many kinds of pH-sensitive polymers in the present invention and all of the basic polymers that expand at low pH can be applied. 0.5 g of chitosan can be dissolved in 50 ml of 0.1% (V / V) acetic acid and EDTA can be added to 3-80% (Wt%) of chitosan to increase strength. To this, 1-5 mg of pigment (D & C Red No. 6 Barium-lake; Warner-Jenkinson, USA) is added and the pH is neutralized with 5M NaOH. EDAC [1-Ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride; Sigma, USA] is added to 10-100 mM. After stirring for 4 hours at room temperature, washed twice with distilled water, washed twice with 0.5 M NaCl, 0.025M NaOH and twice with 0.05M NaOH, and then washed once with distilled water to be refrigerated.
본 발명에서 라벨을 저장공간에서 사용하는 중 온도가 빙점 이하로 내려가는 경우 라벨이 제대로 작동하기 위해서는 빙점 이하에서도 저장물질의 부패균이 생존해 있어야 한다. 본 발명에서 동결건조된 유기산과 pH 지시약을 포함하도록 제조된 라벨을 다양한 온도에 노출한 후 pH 지시약의 색상을 비교함으로써 저장 물질의 부패 판단을 수행할 수 있다. 본 발명의 라벨은 저장 온도변화의 누적에 따른 저장물질의 부패가능성 판단용 라벨로 유용하게 사용될 수 있다.In the present invention, when the temperature falls below the freezing point while using the label in the storage space, the decayed bacteria of the storage material must survive even below the freezing point in order for the label to work properly. In the present invention, the label prepared to include the lyophilized organic acid and the pH indicator may be exposed to various temperatures, and then the corruption of the storage material may be determined by comparing the colors of the pH indicator. The label of the present invention can be usefully used as a label for determining the perishability of the storage material according to the accumulation of storage temperature change.
본 발명에서 pH가 다시 상승하는 경우 pH가 중성으로 될 수 있으므로 pH가 오를 경우에도 색이 변해서는 안 된다. 한 번 낮은 pH에 의해 색이 변하면 그 색이 유지되도록 하는 방법이 필요하다. 본 발명의 금속비누 입자와 pH 민감성 고분자 입자가 더욱 유용하게 이용될 수 있다.In the present invention, when the pH rises again, since the pH may be neutral, the color should not change even when the pH rises. Once a color changes due to a low pH, a method is needed to maintain that color. Metal soap particles and pH sensitive polymer particles of the present invention can be used more usefully.
본 발명의 라벨은 식품 저장 용기의 외부에 접착될 수 있는 형태로 제작될 수 있다. 온도에 의해 부패하는 저장물질의 외부 용기에 직접 붙이는 라벨 형태로 제작됨으로써 저장, 수송 중 저장 공간의 온도가 아닌 저장물질의 실제 온도의 변화를 측정할 수 있다.The label of the present invention may be manufactured in a form that can be adhered to the outside of the food storage container. It is manufactured in the form of a label that is directly attached to the outer container of the storage material that is perished by temperature, so that the change in the actual temperature of the storage material may be measured, not the temperature of the storage space during storage and transportation.
본 발명에서 유산균에 따른 pH 변화 및 pH 표지물질의 색 변화를 확인하기 위하여 유산균을 pH 지시약, 금속비누 입자 및 pH 민감성 고분자 입자를 각각 0.0001%, 1% 및 1%의 농도로 30℃에서 pH 변화 및 pH 표지물질의 색 변화를 확인한다. 마지막에 pH를 중성으로 보정하고 색 변화를 확인한다. pH 지시약의 경우 처음 녹색이던 지시약은 자주색 및 적색으로 변하여 색깔이 유지되며 금속비누 입자나 pH 민감성 고분자 입자의 경우 중성으로 유지해도 색은 붉은 색을 유지한다.In the present invention, in order to confirm the pH change and color change of the pH labeling material according to the lactic acid bacteria, the pH indicator, the metal soap particles, and the pH sensitive polymer particles were changed in pH at 30 ° C. at concentrations of 0.0001%, 1%, and 1%, respectively. And the color change of the pH marker. At the end, calibrate the pH to neutral and check the color change. In the case of the pH indicator, the indicator, which was initially green, turns purple and red to maintain its color, and the color remains red even when the metal soap particles or pH-sensitive polymer particles remain neutral.
본 발명은 라벨을 저장물질의 외부 용기에 부착하고, 저장기간이 경과되면서 외부온도가 -10 내지 40℃로 상승하여 저장물질이 부패되는 경우 용해되는 코팅층 외부온도 -10 내지 40℃에서 선택적으로 용해되는 코팅층(50)이 용해되면서 pH 지시약의 색깔이 변색되고, 변색된 라벨의 색을 관찰하여 산성 색인 경우, 저장물질의 부패 가능으로 판단할 수 있다.The present invention attaches the label to the outer container of the storage material, and selectively dissolves at the outer temperature of the coating layer -10 to 40 ℃ dissolved when the storage material is decayed as the storage temperature is increased to -10 to 40 ℃ as the storage period elapses When the coating layer 50 is dissolved, the color of the pH indicator is discolored, and when the acidic index is observed by observing the color of the discolored label, it may be determined that the storage material may be decayed.
본 발명에서 저장온도 변화에 따른 색 변화를 확인하기 위하여 제조한 라벨을 -10℃, 0℃, 4℃, 5℃, 10℃, 15℃, 20℃, 25℃, 30℃, 35℃, 37℃ 및 40℃의 다양한 온도에 정해진 시간만큼 노출한 후 각 구간에서 색 변화를 관찰한다. 지시약의 색 변화가 나타나면 색 변화를 바탕으로 부패 가능성을 판단할 수 있다.In the present invention, the label prepared to confirm the color change according to the change of the storage temperature is -10 ℃, 0 ℃, 4 ℃, 5 ℃, 10 ℃, 15 ℃, 20 ℃, 25 ℃, 30 ℃, 35 ℃, 37 After exposure to various temperatures of ℃ and 40 ℃ for a predetermined time and observe the color change in each section. If the indicator changes color, the possibility of corruption can be determined based on the color change.
본 발명에서 라벨의 표면코팅층(40)은 접착시 가하는 압력에 대해서 내부 물질을 보호할 수 있도록 PE(poly ethylene), PP(poly propylene), PS(Poly styrene), PET(Poly Ethylene Telephthalate) 등의 재질을 필름형태로 만들어 사용하며 접착 하단면은 저장물질의 색과 혼돈되지 않도록 불투명 백색을 사용하는 것이 바람직하다.In the present invention, the surface coating layer 40 of the label is PE (polyethylene), PP (poly propylene), PS (Poly styrene), PET (Poly Ethylene Telephthalate), etc. to protect the internal material against the pressure applied during adhesion It is preferable to use opaque white to make the material in the form of a film and to avoid confusion with the color of the storage material.
[규칙 제26조에 의한 보정 29.06.2015]　
[DELETE][Revision 29.06.2015 under Rule 26]
[DELETE]

Claims (10)

  1. 라벨(10)의 상부표면에 부착되어 있는 pH 지시약층(24)과 상기 pH 지시약층(24)의 상부에 부착된 유산균 코팅코아층(38); 및A pH indicator layer 24 attached to the upper surface of the label 10 and a lactic acid bacteria coating core layer 38 attached to the top of the pH indicator layer 24; And
    라벨(10)의 상부표면에 부착되어 있는 유산균 코팅코아층(38)과 상기 유산균 코팅코아층(38)의 상부에 부착된 pH 지시약층(24); 중에서 선택된 어느 하나의 2층으로 이루어져 있으며,A lactic acid bacteria coating core layer 38 attached to the upper surface of the label 10 and a pH indicator layer 24 attached to the upper portion of the lactic acid bacteria coating core layer 38; It consists of any two layers selected from,
    상기 pH 지시약층(24)은 pH 지시약 분말(20) 및 pH 지시약 액체(22) 중에서 선택된 어느 하나의 지시약으로 이루어져 있고, The pH indicator layer 24 is composed of any one indicator selected from the pH indicator powder 20 and the pH indicator liquid 22,
    상기 유산균 코팅코아층(38)은 유산균 코팅코아(36)의 내부에 유산균 액체(32)가 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 유산균 액체층의 외부에 코팅된 다수개의 유산균 코팅코아(36)로 이루어진 것이며,The lactic acid bacteria coating core layer 38 is a lactic acid bacteria liquid 32 is embedded in the lactic acid bacteria coating core 36, the outer layer of the lactic acid bacteria liquid layer as a coating layer 50 for the external shock detection that the coating layer is broken by an external impact. It consists of a plurality of coated lactic acid bacteria coating core (36),
    상기 코팅층(50)은 당, 전분, 상전이 코팅제 및 상전이유도 촉매로 이루어져 있으며, 상기 상전이 코팅제는 아마씨를 100℃에서 20분 동안 가열하여 아마씨에서 수분을 제거한 후 급속 냉각시키고, 급속 냉각된 아마씨를 착유기에 넣고 착유기의 온도 100℃ 상태에서 착유한 후 200 메쉬에서 여과하여 여과된 아마씨유를 수득하고, 상기 여과된 아마씨유를 20일 자연침전시킨 후 상층부를 회수하는 공정을 거쳐 정제된 아마씨유를 수득하여 제조된 악취 및 시안아이드(-CN) 독성이 제거된 아마씨유인 것을 특징으로 하는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨.The coating layer 50 is composed of sugar, starch, phase-transfer coating agent and phase transition induction catalyst, the phase-transfer coating agent is heated for 20 minutes at 100 ℃ to remove moisture from flaxseed and then rapidly cooled, milking the fast-cooled flaxseed And milked at a temperature of the milking machine at a temperature of 100 ℃ and then filtered in 200 mesh to obtain a filtered flaxseed oil, and the purified flaxseed oil through a process of spontaneously precipitating the filtered flaxseed oil for 20 days to recover the upper layer. Functional label for external shock detection lactic acid bacteria for the freshness check of the storage material, characterized in that the odor and cyanide (-CN) toxicity prepared by removing flaxseed oil.
  2. 라벨(10)의 상부표면에 부착되어 있는 pH 지시약층(24)과 상기 pH 지시약층(24)의 상부에 부착된 유산균 코팅코아층(38); 및A pH indicator layer 24 attached to the upper surface of the label 10 and a lactic acid bacteria coating core layer 38 attached to the top of the pH indicator layer 24; And
    라벨(10)의 상부표면에 부착되어 있는 유산균 코팅코아층(38)과 상기 유산균 코팅코아층(38)의 상부에 부착된 pH 지시약층(24); 중에서 선택된 어느 하나의 2층으로 이루어져 있으며,A lactic acid bacteria coating core layer 38 attached to the upper surface of the label 10 and a pH indicator layer 24 attached to the upper portion of the lactic acid bacteria coating core layer 38; It consists of any two layers selected from,
    상기 pH 지시약층(24)은 pH 지시약 액체(22)로 이루어져 있고, The pH indicator layer 24 is composed of a pH indicator liquid 22,
    상기 유산균 코팅코아층(38)은 유산균 코팅코아(36)의 내부에 유산균 분말(30)이 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 유산균 분말층의 외부에 코팅된 다수개의 유산균 코팅코아(36)로 이루어져 있고,The lactic acid bacteria coating core layer 38 is a lactic acid bacteria powder 30 is embedded in the lactic acid bacteria coating core 36, the coating layer for the external impact detection layer 50 is broken by an external impact on the outside of the lactic acid bacteria powder layer. It consists of a plurality of coated lactic acid bacteria coating core (36),
    상기 코팅층(50)은 당, 전분, 상전이 코팅제 및 상전이유도 촉매로 이루어져 있으며, 상기 상전이 코팅제는 아마씨를 100℃에서 20분 동안 가열하여 아마씨에서 수분을 제거한 후 급속 냉각시키고, 급속 냉각된 아마씨를 착유기에 넣고 착유기의 온도 100℃ 상태에서 착유한 후 200 메쉬에서 여과하여 여과된 아마씨유를 수득하고, 상기 여과된 아마씨유를 20일 자연침전시킨 후 상층부를 회수하는 공정을 거쳐 정제된 아마씨유를 수득하여 제조된 악취 및 시안아이드(-CN) 독성이 제거된 아마씨유인 것을 특징으로 하는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨.The coating layer 50 is composed of sugar, starch, phase-transfer coating agent and phase transition induction catalyst, the phase-transfer coating agent is heated for 20 minutes at 100 ℃ to remove moisture from flaxseed and then rapidly cooled, milking the fast-cooled flaxseed And milked at a temperature of the milking machine at a temperature of 100 ℃ and then filtered in 200 mesh to obtain a filtered flaxseed oil, and the purified flaxseed oil through a process of spontaneously precipitating the filtered flaxseed oil for 20 days to recover the upper layer. Functional label for external shock detection lactic acid bacteria for the freshness check of the storage material, characterized in that the odor and cyanide (-CN) toxicity prepared by removing flaxseed oil.
  3. 라벨(10)의 상부표면에 부착되어 있는 pH 지시약 코팅코아층(28)과 상기 pH 지시약 코팅코아층(28)의 상부에 부착된 유산균층(34); 및A pH indicator coating core layer 28 attached to the upper surface of the label 10 and a lactic acid bacterium layer 34 attached to the top of the pH indicator coating core layer 28; And
    라벨(10)의 상부표면에 부착되어 있는 유산균층(34)과 유산균 코팅코아층(38)의 상부에 부착된 pH 지시약 코팅코아층(28); 중에서 선택된 어느 하나의 2층으로 이루어져 있으며,A pH indicator coating core layer 28 attached on top of the lactic acid bacteria layer 34 and the lactic acid bacteria coating core layer 38 attached to the upper surface of the label 10; It consists of any two layers selected from,
    상기 유산균층(34)은 유산균 분말(30) 및 유산균 액체(32) 중에서 선택된 어느 하나의 유산균으로 이루어져 있고, The lactic acid bacteria layer 34 is composed of any one lactic acid bacteria selected from lactic acid bacteria powder 30 and the liquid lactic acid bacteria 32,
    상기 pH 지시약 코팅코아층(28)은 pH 지시약 코팅코아(26)의 내부에 pH 지시약 액체(22)가 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 pH 지시약 액체층의 외부에 코팅된 다수개의 pH 지시약 코팅코아(26)로 이루어져 있고,The pH indicator coating core layer 28 is a pH indicator liquid 22 is embedded in the pH indicator coating core 26, the pH indicator liquid as the coating layer 50 for the external shock detection that the coating layer is broken by an external impact. A plurality of pH indicator coating cores 26 coated on the outside of the layer,
    상기 코팅층(50)은 당, 전분, 상전이 코팅제 및 상전이유도 촉매로 이루어져 있으며, 상기 상전이 코팅제는 아마씨를 100℃에서 20분 동안 가열하여 아마씨에서 수분을 제거한 후 급속 냉각시키고, 급속 냉각된 아마씨를 착유기에 넣고 착유기의 온도 100℃ 상태에서 착유한 후 200 메쉬에서 여과하여 여과된 아마씨유를 수득하고, 상기 여과된 아마씨유를 20일 자연침전시킨 후 상층부를 회수하는 공정을 거쳐 정제된 아마씨유를 수득하여 제조된 악취 및 시안아이드(-CN) 독성이 제거된 아마씨유인 것을 특징으로 하는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨.The coating layer 50 is composed of sugar, starch, phase-transfer coating agent and phase transition induction catalyst, the phase-transfer coating agent is heated for 20 minutes at 100 ℃ to remove moisture from flaxseed and then rapidly cooled, milking the fast-cooled flaxseed And milked at a temperature of the milking machine at a temperature of 100 ℃ and then filtered in 200 mesh to obtain a filtered flaxseed oil, and the purified flaxseed oil through a process of spontaneously precipitating the filtered flaxseed oil for 20 days to recover the upper layer. Functional label for external shock detection lactic acid bacteria for the freshness check of the storage material, characterized in that the odor and cyanide (-CN) toxicity prepared by removing flaxseed oil.
  4. 라벨(10)의 상부표면에 부착되어 있는 pH 지시약 코팅코아층(28)과 상기 pH 지시약 코팅코아층(28)의 상부에 부착된 유산균층(34); 및A pH indicator coating core layer 28 attached to the upper surface of the label 10 and a lactic acid bacterium layer 34 attached to the top of the pH indicator coating core layer 28; And
    라벨(10)의 상부표면에 부착되어 있는 유산균층(34)과 유산균 코팅코아층(38)의 상부에 부착된 pH 지시약 코팅코아층(28); 중에서 선택된 어느 하나의 2층으로 이루어져 있으며,A pH indicator coating core layer 28 attached on top of the lactic acid bacteria layer 34 and the lactic acid bacteria coating core layer 38 attached to the upper surface of the label 10; It consists of any two layers selected from,
    상기 유산균층(34)은 유산균 분말(30)로 이루어져 있고, The lactic acid bacteria layer 34 is made of lactic acid bacteria powder 30,
    상기 pH 지시약 코팅코아층(28)은 pH 지시약 코팅코아(26)의 내부에 pH 지시약 액체(22)가 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 pH 지시약 액체층의 외부에 코팅된 다수개의 pH 지시약 코팅코아(26)로 이루어져 있고,The pH indicator coating core layer 28 is a pH indicator liquid 22 is embedded in the pH indicator coating core 26, the pH indicator liquid as the coating layer 50 for the external shock detection that the coating layer is broken by an external impact. A plurality of pH indicator coating cores 26 coated on the outside of the layer,
    상기 코팅층(50)은 당, 전분, 상전이 코팅제 및 상전이유도 촉매로 이루어져 있으며, 상기 상전이 코팅제는 아마씨를 100℃에서 20분 동안 가열하여 아마씨에서 수분을 제거한 후 급속 냉각시키고, 급속 냉각된 아마씨를 착유기에 넣고 착유기의 온도 100℃ 상태에서 착유한 후 200 메쉬에서 여과하여 여과된 아마씨유를 수득하고, 상기 여과된 아마씨유를 20일 자연침전시킨 후 상층부를 회수하는 공정을 거쳐 정제된 아마씨유를 수득하여 제조된 악취 및 시안아이드(-CN) 독성이 제거된 아마씨유인 것을 특징으로 하는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨.The coating layer 50 is composed of sugar, starch, phase-transfer coating agent and phase transition induction catalyst, the phase-transfer coating agent is heated for 20 minutes at 100 ℃ to remove moisture from flaxseed and then rapidly cooled, milking the fast-cooled flaxseed And milked at a temperature of the milking machine at a temperature of 100 ℃ and then filtered in 200 mesh to obtain a filtered flaxseed oil, and the purified flaxseed oil through a process of spontaneously precipitating the filtered flaxseed oil for 20 days to recover the upper layer. Functional label for external shock detection lactic acid bacteria for the freshness check of the storage material, characterized in that the odor and cyanide (-CN) toxicity prepared by removing flaxseed oil.
  5. 라벨(10)의 상부표면에 부착되어 있는 pH 지시약 코팅코아층(28)과 상기 pH 지시약 코팅코아층(28)의 상부에 부착된 유산균 코팅코아층(38); 및A pH indicator coating core layer 28 attached to the upper surface of the label 10 and a lactic acid bacteria coating core layer 38 attached to the top of the pH indicator coating core layer 28; And
    라벨(10)의 상부표면에 부착되어 있는 유산균 코팅코아층(38)과 상기 유산균 코팅코아층(38)의 상부에 부착된 pH 지시약 코팅코아층(28); 중에서 선택된 어느 하나의 2층으로 이루어져 있으며,A lactic acid bacteria coating core layer 38 attached to the upper surface of the label 10 and a pH indicator coating core layer 28 attached to the upper portion of the lactic acid bacteria coating core layer 38; It consists of any two layers selected from,
    상기 pH 지시약 코팅코아층(28)은 pH 지시약 코팅코아(26)의 내부에 pH 지시약 분말(20) 및 pH 지시약 액체(22) 중에서 선택된 어느 하나의 지시약이 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 pH 지시약층의 외부에 코팅된 다수개의 pH 지시약 코팅코아(26)로 이루어져 있으며,The pH indicator coating core layer 28 has any one indicator selected from the pH indicator powder 20 and the pH indicator liquid 22 inside the pH indicator coating core 26, and the coating layer is broken by an external impact. The external shock detection coating layer 50 is composed of a plurality of pH indicator coating core 26 coated on the outside of the pH indicator layer,
    상기 유산균 코팅코아층(38)은 유산균 코팅코아(36)의 내부에 유산균 액체(32)가 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 유산균 액체층의 외부에 코팅된 다수개의 유산균 코팅코아(36)로 이루어진 것이고,The lactic acid bacteria coating core layer 38 is a lactic acid bacteria liquid 32 is embedded in the lactic acid bacteria coating core 36, the outer layer of the lactic acid bacteria liquid layer as a coating layer 50 for the external shock detection that the coating layer is broken by an external impact. It consists of a plurality of coated lactic acid bacteria coating core 36,
    상기 코팅층(50)은 당, 전분, 상전이 코팅제 및 상전이유도 촉매로 이루어져 있으며, 상기 상전이 코팅제는 아마씨를 100℃에서 20분 동안 가열하여 아마씨에서 수분을 제거한 후 급속 냉각시키고, 급속 냉각된 아마씨를 착유기에 넣고 착유기의 온도 100℃ 상태에서 착유한 후 200 메쉬에서 여과하여 여과된 아마씨유를 수득하고, 상기 여과된 아마씨유를 20일 자연침전시킨 후 상층부를 회수하는 공정을 거쳐 정제된 아마씨유를 수득하여 제조된 악취 및 시안아이드(-CN) 독성이 제거된 아마씨유인 것을 특징으로 하는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨.The coating layer 50 is composed of sugar, starch, phase-transfer coating agent and phase transition induction catalyst, the phase-transfer coating agent is heated for 20 minutes at 100 ℃ to remove moisture from flaxseed and then rapidly cooled, milking the fast-cooled flaxseed And milked at a temperature of the milking machine at a temperature of 100 ℃ and then filtered in 200 mesh to obtain a filtered flaxseed oil, and the purified flaxseed oil through a process of spontaneously precipitating the filtered flaxseed oil for 20 days to recover the upper layer. Functional label for external shock detection lactic acid bacteria for the freshness check of the storage material, characterized in that the odor and cyanide (-CN) toxicity prepared by removing flaxseed oil.
  6. 라벨(10)의 상부표면에 부착되어 있는 pH 지시약 코팅코아층(28)과 상기 pH 지시약 코팅코아층(28)의 상부에 부착된 유산균 코팅코아층(38); 및A pH indicator coating core layer 28 attached to the upper surface of the label 10 and a lactic acid bacteria coating core layer 38 attached to the top of the pH indicator coating core layer 28; And
    라벨(10)의 상부표면에 부착되어 있는 유산균 코팅코아층(38)과 상기 유산균 코팅코아층(38)의 상부에 부착된 pH 지시약 코팅코아층(28); 중에서 선택된 어느 하나의 2층으로 이루어져 있으며,A lactic acid bacteria coating core layer 38 attached to the upper surface of the label 10 and a pH indicator coating core layer 28 attached to the upper portion of the lactic acid bacteria coating core layer 38; It consists of any two layers selected from,
    상기 pH 지시약 코팅코아층(28)은 pH 지시약 코팅코아(26)의 내부에 pH 지시약 액체(22)이 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 pH 지시약층의 외부에 코팅된 다수개의 pH 지시약 코팅코아(26)로 이루어져 있으며,The pH indicator coating core layer 28 is a pH indicator liquid 22 is embedded in the pH indicator coating core 26, the pH indicator layer as an external impact detection coating layer 50 is broken by an external impact. Consists of a plurality of pH indicator coating core (26) coated on the outside of,
    상기 유산균 코팅코아층(38)은 유산균 코팅코아(36)의 내부에 유산균 분말(32)이 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 유산균 분말층의 외부에 코팅된 다수개의 유산균 코팅코아(36)로 이루어진 것이고,The lactobacillus coating core layer 38 is a lactic acid bacteria powder 32 is embedded in the lactic acid bacteria coating core 36, the outer layer of the lactic acid bacteria powder layer as an external impact detection coating layer 50 is broken by an external impact. It consists of a plurality of coated lactic acid bacteria coating core 36,
    상기 코팅층(50)은 당, 전분, 상전이 코팅제 및 상전이유도 촉매로 이루어져 있으며, 상기 상전이 코팅제는 아마씨를 100℃에서 20분 동안 가열하여 아마씨에서 수분을 제거한 후 급속 냉각시키고, 급속 냉각된 아마씨를 착유기에 넣고 착유기의 온도 100℃ 상태에서 착유한 후 200 메쉬에서 여과하여 여과된 아마씨유를 수득하고, 상기 여과된 아마씨유를 20일 자연침전시킨 후 상층부를 회수하는 공정을 거쳐 정제된 아마씨유를 수득하여 제조된 악취 및 시안아이드(-CN) 독성이 제거된 아마씨유인 것을 특징으로 하는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨.The coating layer 50 is composed of sugar, starch, phase-transfer coating agent and phase transition induction catalyst, the phase-transfer coating agent is heated for 20 minutes at 100 ℃ to remove moisture from flaxseed and then rapidly cooled, milking the fast-cooled flaxseed And milked at a temperature of the milking machine at a temperature of 100 ℃ and then filtered in 200 mesh to obtain a filtered flaxseed oil, and the purified flaxseed oil through a process of spontaneously precipitating the filtered flaxseed oil for 20 days to recover the upper layer. Functional label for external shock detection lactic acid bacteria for the freshness check of the storage material, characterized in that the odor and cyanide (-CN) toxicity prepared by removing flaxseed oil.
  7. 라벨(10)의 상부표면에 pH 지시약 코팅코아(26)과 유산균 코팅코아(36)의 혼합층이 부착되어 있으며,On the upper surface of the label 10, a mixed layer of pH indicator coating core 26 and lactic acid bacteria coating core 36 is attached.
    상기 pH 지시약 코팅코아(26)는 pH 지시약 코팅코아(26)의 내부에 pH 지시약 분말(20) 및 pH 지시약 액체(22) 중에서 선택된 어느 하나의 지시약이 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 pH 지시약층의 외부에 코팅되어 있고,The pH indicator coating core 26 has any one indicator selected from the pH indicator powder 20 and the pH indicator liquid 22 inside the pH indicator coating core 26, the outer coating layer is broken by an external impact. It is coated on the outside of the pH indicator layer with a shock-sensitive coating layer 50,
    상기 유산균 코팅코아(36)는 유산균 코팅코아(36)의 내부에 유산균 액체(32)가 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 유산균 액체층의 외부에 코팅되어 있는 것을 특징으로 하는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨.The lactic acid bacteria coating core 36 is a lactic acid bacteria liquid 32 is embedded in the lactic acid bacteria coating core 36, the coating layer 50 for the external shock detection that the coating layer is broken by an external impact is coated on the outside of the lactic acid bacteria liquid layer. Lactobacillus coating functional label for external shock detection for checking the freshness of the storage material, characterized in that.
  8. 라벨(10)의 상부표면에 pH 지시약 코팅코아(26)과 유산균 코팅코아(36)의 혼합층이 부착되어 있으며,On the upper surface of the label 10, a mixed layer of pH indicator coating core 26 and lactic acid bacteria coating core 36 is attached.
    상기 pH 지시약 코팅코아(26)는 pH 지시약 코팅코아(26)의 내부에 pH 지시약 액체(22)가 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 pH 지시약층의 외부에 코팅되어 있고,The pH indicator coating core 26 is a pH indicator liquid 22 is built in the pH indicator coating core 26, the coating layer for the external shock detection coating layer 50 is broken by an external impact of the pH indicator layer of Coated on the outside,
    상기 유산균 코팅코아(36)는 유산균 코팅코아(36)의 내부에 유산균 분말(30)이 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 유산균 액체층의 외부에 코팅되어 있는 것을 특징으로 하는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨.The lactic acid bacteria coating core 36 is a lactic acid bacterium coating core 36 is embedded in the lactic acid bacteria powder 30, the coating layer 50 for the external shock detection that the coating layer is broken by an external impact is coated on the outside of the lactic acid bacteria liquid layer. Lactobacillus coating functional label for external shock detection for checking the freshness of the storage material, characterized in that.
  9. 라벨(10)의 상부표면에 pH 지시약 분말(20)과 유산균 코팅코아(36)의 혼합층이 부착되어 있으며,On the upper surface of the label 10, a mixed layer of pH indicator powder 20 and lactic acid bacteria coating core 36 is attached.
    상기 유산균 코팅코아(36)는 유산균 코팅코아(36)의 내부에 유산균 액체(32)가 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 유산균 액체층의 외부에 코팅되어 있는 것을 특징으로 하는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨.The lactic acid bacteria coating core 36 is a lactic acid bacteria liquid 32 is embedded in the lactic acid bacteria coating core 36, the coating layer 50 for the external shock detection that the coating layer is broken by an external impact is coated on the outside of the lactic acid bacteria liquid layer. Lactobacillus coating functional label for external shock detection for checking the freshness of the storage material, characterized in that.
  10. 라벨(10)의 상부표면에 pH 지시약 코팅코아(26)과 유산균 분말(30)의 혼합층이 부착되어 있으며,On the upper surface of the label 10, a mixed layer of pH indicator coating core 26 and lactic acid bacteria powder 30 is attached,
    상기 pH 지시약 코팅코아(26)은 pH 지시약 코팅코아(26)의 내부에 pH 지시약 액체(22)가 내장되어 있으며, 외부 충격으로 코팅층이 깨지는 외부충격감지용 코팅층(50)으로 상기 pH 지시약층의 외부에 코팅되어 있는 것을 특징으로 하는 저장물질의 신선도 체크용 외부충격감지 유산균 코팅 기능성 라벨.The pH indicator coating core 26 is a pH indicator liquid 22 is embedded in the pH indicator coating core 26, the coating layer for the external shock detection coating 50 is broken by an external impact of the pH indicator layer of Lactobacillus coating functional label for external shock detection for checking the freshness of the storage material, characterized in that the coating on the outside.
PCT/KR2015/005702 2014-06-09 2015-06-08 Functional label, having lactobacillus coating for detecting external impact, for checking freshness of storage material WO2015190772A1 (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110564013A (en) * 2019-08-05 2019-12-13 武汉大学 Preparation method and sensitivity regulation method of pH type food freshness indication label

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020066157A (en) * 2001-02-09 2002-08-14 박찬훈 A bio-indicator for monitoring of microbial decomposition
KR101012125B1 (en) * 2008-07-24 2011-02-07 인하대학교 산학협력단 Label for estimating of saprogenic bacteria's growth by cumulative storage temperature' change
KR20110086950A (en) * 2010-01-25 2011-08-02 신봉석 Composition for aques solution of flax seed oil comprising omega 3-fatic acid

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020066157A (en) * 2001-02-09 2002-08-14 박찬훈 A bio-indicator for monitoring of microbial decomposition
KR101012125B1 (en) * 2008-07-24 2011-02-07 인하대학교 산학협력단 Label for estimating of saprogenic bacteria's growth by cumulative storage temperature' change
KR20110086950A (en) * 2010-01-25 2011-08-02 신봉석 Composition for aques solution of flax seed oil comprising omega 3-fatic acid

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110564013A (en) * 2019-08-05 2019-12-13 武汉大学 Preparation method and sensitivity regulation method of pH type food freshness indication label

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