KR100975020B1 - The process for cultivating chlorella that can be drinkable and contains the effective ingredients of the traditional oriental medicine by generating the vortex - Google Patents

Abstract

The present invention is to provide a method for producing chlorella that can be cultured and drinking chlorella containing medicinal herbs containing the active ingredient by vortex also improve the freshness to have high activity when absorbed or otherwise attached to the human body In the culturing process of chlorella according to the present invention, the herbal medicine was administered to cultivate and propagate chlorella containing various active ingredients of oriental medicine, thereby expanding the potential for various applications including the human body.

Vortex. Chlorella. Chinese herbal medicine. plasma. beverage

Description

The process for cultivating chlorella that can be drinkable and contains the effective ingredients of the traditional oriental medicine by generating the vortex}

1 is a representation of a method of generating a vortex

단계의 본 배양장의 배양수 증량도2

Increase of culture water of main culture stage of step

단계에서 노즐 분사각과 배양수 증량 단계도3 is

Nozzle nozzle angle and culture water

4 is a vortex generation degree of a small dose chlorella culture tank

5-1.2.3 is the operation of the primary culture

6-1 is a front view of the present culture center

Figure 6-2 is a side view of the present culture

7 is a conceptual diagram of chlorella's activity

8 is an assembly view of the present culture

9 is a flow chart of the FRP treatment operation of the present culture plant

10 is a chlorella cycle

11 is a manifestation cycle

Description of the Related Art

 P: Pump A: Oxygen Generator

Vortex: Centripetal Spiral Movement

S: south N: north

E: east W: west

1: Bottom shape of small volume culture tank

2: bottom shape of large capacity culture tank

3: Harvester of Chlorella Culture Water

a: Support tibia b: External bulkhead c: Overlap of the external bulkhead

def g: specific gravity and order of FRP processing
A: First culture level in CLO2 stage
B: Secondary culture level at CLO2 stage
C: 3rd culture level and culture completion level of CLO2 stage

[Patent 1] Korean Patent No. 1998-013379. 1998.04.10

[Patent 2] Korea Patent 10-2003-0035158. 2003.5.31

The present invention relates to a method for culturing and manufacturing liquid chlorella, and the like, including herbal medicines, and generating vortex to increase cultivation efficiency and to provide chlorella, which has been exposed to a lot of usefulness in humans and the environment. The present invention relates to a method for cultivating chlorella, which helps a lot in human life by establishing a system of culture technology. According to recent reports, heavy metals are blocked. Osteoporosis prevention. Promotes Child Development. Atopy improvement. Cosmetic medicines. Miso. tofu. Many applications and benefits have been reported for food products such as kimchi. However, in contrast to many test results, domestic production base was very weak and stable production technology was not accumulated. Especially, due to the inherent taste of chlorella, many applications of beverages were limited. The present application is to increase the efficiency by providing a chlorella number that can control the smell and drink using herbal medicines, and by culturing the chlorella suitable for individual use.

군으로부터 공명에 의한 명령이

군을 거쳐

군으로 전달된다.As shown in Fig. 7, when chlorella grows between PH 5 and 8.5, each of them causes resonance and metabolizes, and a variable that affects the growth environment occurs.

Resonant orders from the military

By military

Delivered to the military.

In general, group c is mainly young or old, and it is mainstream, and it is settled to the invaders or sinks to the bottom to maintain a constant and stable environment at all times. Bird ecology and application of Korea; Lee In-gyu and others. The absorbed heavy metal is said to be transformed into a stable substance such as biotransformation. In this regard, the applicant is regarded as disassembling and recombining molecular bond structures such as organic compounds through metabolism of oxidation and reduction. Chlorella has a size of 2 ~ 12㎛ and is classified as a plant green algae that lives mainly in sea water and fresh water. In this application, VULGARIS species is used as the main raw material, but the scope of application is not limited to Chlorella. In addition, chlorella's CGF (CHLORELLA GROWTH FACTOR) is essential for its physiological activity (antitumor antibacterial, antifungal, neuroactive). Increased resistance to disease. It is pointed out as a factor that enables change in the constitution. PYRENODOISA is a representative species that is currently cultivated in outdoor culture in Taiwan, Japan, and the like, and the United States, which has found chlorella earlier and tried to commercialize it. Japan. Taiwan. Pakistan. Many countries, including Bangladesh, use PYRENODOISA species mainly because of their subtropical climate. Due to the inherent odor of chlorella, there were many difficulties in commercializing the liquid phase, so that the culture water was passed through a centrifuge and dehydrated using a high-speed rotation of 8000 to 20.000 rpm, followed by drying and powdering. Granules. Extender through secondary processing through molding in the form of a powder. Add a colorant, etc. to make tablets (pills) through a tableting machine and coat them to complete the product. Another method is the cow culture method, which shows that the chlorella's photosynthetic ability is 6-12 times higher than that of ordinary plants. And the outdoor cultivation method is acid rain due to changes in the global atmospheric environment. Dust. Fallout is not safe from invasion of birds or insects, so in this application, glass greenhouse. plastic house. Steel structures and transparent plastics (acryl. PP. PC) were used. It is a compromise that combines the advantages of actively using sunlight from outdoor culture methods and the cleanliness of cow culture.

단계로 2ℓ∼30.000ℓ 용량의 1차 배양장에 감초. 인진쑥. 도라지. 사상자. 인삼. 천초 옻. 귤피. 대조 등을 배양수 1ℓ당 0.2∼30g 중량으로 열수추출 또는 ABC 하고 무명 또는 거름망 등에 약재를 담아서 담궈준다It is an object of the present invention to provide a first drinking chlorella. The second purpose is to provide chlorella to eat and apply. Thirdly, the aim is to provide customized chlorella for individual characteristics. Fourth, the human body. Environment. plant. It is intended to contribute to the promotion of human health by securing mass culture technology such as chlorella, which has been reported to have many usefulness in animals, etc. In order to achieve the above object, the chlorella culture method of the present invention

Licorice in the primary culture of 2 l to 30.000 l volume in the step. Injin mugwort. Bellflower. Casualties. Ginseng. Cheoncho lacquer. Tangerines. Extract control or hot water with 0.2 ~ 30g weight per 1ℓ of culture water, and dip it in cotton or strainer

Chlorella powder is also put in a sieve, etc., aerated for 1-7 days, and cultured with shaking by adding an organic medium at 15 ° to 35 ° C. After 1-3 days, take out the medicine and raw sieve. Plasma. Pharmacological components are injected into the chlorella at the same time as irradiation with laser irradiation ozone or UV lamp. Incubate with aeration for 1 to 5 days. Meanwhile, 1 to 10 tons of purified or cultured water is added to the culture center and exposed to direct sunlight or ultraviolet rays for 1 to 7 days.

단계 본 배양장에서 (도 3의 가)지점이 되는데 아주 약하게 배양수를 회전시키거나 성장이 원활하다면 그대로 둔다 1일∼7일이 지난후 배양장 온도 15°∼35°이며 오전일 경우 8:30∼11:30분 오후에는 13:30∼17:30분에 1차 식이를 공급한다 조도는 1000LUX 이상이며 식이는 1∼7일에 한번꼴로 공급하고 이때는 클로렐라가 본배양장과 주변 환경에 적응하는 시기이므로 세심한 주의가 요구된다. 1차∼5차 식이 공급에서 청록색 또는 진녹색으로 변하며 PH 6∼8.5가 되었고 현미경 등으로 보았을 때 활발한 분화가 일어나고 있다면 추가 배양수를 공급한다. 2차 배양수 공급시 매우 약하게 분사 또는 분무되게 하며 교반용 모터를 가동하여 보텍스를 발생시킨다 (도 3의 나지점)(Hereinafter referred to as culture water). The activity is increased by 1000 ~ 1500 magnification optical microscope or electron microscope.

Step (A) in Figure 3 (a) is the point in this culture, if the rotation of the culture water is very weak or if the growth is smooth, leave it as it is. After 1 to 7 days, the culture temperature is 15 ° to 35 ° and in the morning 8: 30 ~ 11: 30 minutes In the afternoon, supply the first diet at 13: 30 ~ 17: 30. The illuminance is over 1000LUX and the diet is supplied once every 1 ~ 7 days. It is a time of adaptation, so close attention is required. In the first to fifth dietary feeds, turn green or dark green to a pH of 6 to 8.5, and if active differentiation occurs under a microscope, supply additional culture water. When supplying the second culture water is very weakly sprayed or sprayed to generate a vortex by operating a motor for stirring (naji point of Figure 3)

2 hours to 84 hours after the height of the cultured water reaches point b, the chlorella is observed by feeding the diet and if the active activity is observed by examining the activity under a microscope or the like, additionally add the cultured water.

Continue to generate vortex and start the stirring motor until it reaches the point of (C) of FIG. 3 and stir for 1 to 7 days when it reaches the point of (C) of FIG. 3, supply the diet at an appropriate time, and induce proliferation. It is transferred to the intestine for aging, and the period of adaptation for distribution is given for 1 to 7 days.

단계 PET. 캔. 병. 통. 50㎖. 1ℓ. 1.5ℓ. 1.8ℓ 4ℓ. 20ℓ 등의 용기에 주입기를 통해 주입하고 포장. 냉장저장한다.

Step PET. Cans. bottle. barrel. 50 ml. 1ℓ. 1.5 L. 1.8 l 4 l. Inject into a container, such as 20ℓ, via the injector and package it. Refrigerate.

단계로 유통 및 출하한다

Distribute and ship in stages

단계는 배양수를 원심분리기를 사용하여 원심분리하고 생산된 원말을 냉동저장. 동결 건조 방식으로 건조하여 타정. 엑기스 식품첨가용. 의료용. 원말형태 등 다양한 가공방법을 사용할 수 있다.

In the step, centrifuge the culture water using a centrifuge and freeze the produced raw material. Tableting by drying by freeze drying method. For extract food. For medical use. Various processing methods, such as a raw form, can be used.

A feature of the present application is that it is possible to produce a unique taste-free powder without the additives in the raw material processing process

However, the original powder is characterized in that it is always stored frozen.

The implementation of the present invention uses a Bulgari species and the application area is an example carried out in the southern provinces of the Republic of Korea and the scope of application is not limited only to the embodiments of the present invention and in the description the parts necessary to understand the culture method according to the present invention Only the description will be made. The cultivation method of the present invention is a semi-continuous cultivation method, which proliferates and harvests chlorella in a certain cycle, harvests the rinsing plant, cleans the cultivation field, and receives culture water again. Part of the chlorella that had been incubated in the cultivation system was re-incubated to continuously execute the first command. The chlorella differentiation method is generally divided into four parts and is characterized by asexual reproduction. However, in the season change again, the culture method including shaking culture such as primary culture method is carried out again. This is a consideration of the region having four seasons since the embodiment is southern of Korea and there are four seasons.

단계. 목적에 알맞는 한방 약재를 선정하고 중탕하여 열수추출하는데 첫째 체질과 혈액형 등을 고려하여 증세에 알맞은 약재를 (표 1)을 참조하여 선택한다. 물 1ℓ당 0.2g∼30g 까지 투입한다. 물 또는 물과 클로렐라 수를 혼용하거나 클로렐라수 단독투입(ABC)될수 있다 이때의 클로렐라 수의 클로렐라 함량은 0.002∼3.0％ 까지이다. 약탕기 또는 중탕기에 여과망이나 무명 등으로 약재를 감싼후 62°∼200℃ 로 30분∼2시간 50분 까지 가열하고 그 약재와 약액을 1차 배양장에 배양수를 1/3 이상 채운후 투입하는 것을 특징으로 한다. 주요 약재는 마늘(대산). 귤피. 사상자 인진(한인진). 길경(도라지). 옻. 인삼. 산삼. 금은화(인동초 꽃). 오가피 옥죽(둥글레). 대조(대추). 석권백(바위손). 천초(산초). 매실. 유자 당귀. 갈근. 유근피(느릎나무 뿌리껍질). 상백피. 헛깨. 초피. 황기. 쑥. 은행 생강. 소태나무. 두충. 삼지구엽초. 구기자. 권백(부처손). 탱자. 콩 이며 개별 약재의 성분, 효능, 역활은 표 1에서 참고 할수 있다. 역활은 △. □. ○ 로 구분하였으며 △ 는 살충. 살균. 탈취 등 클로렐라가 완전히 활성화 되기전 배양환경의 안정화를 시키며 클로렐라의 성장에 동조하여 대사 공학적 측면에서 중요한 1차적 기전으로 파악되고 있다.In addition, asexual reproduction is not observed to carry out the command indefinitely, but it is observed to have a persistence of about 6 months. First, the production process of the primary, main culture and shipping culture will be described. The material of the primary culture is FRP. Stainless steel plastics (PVC, acrylic, PC, PE, PET), which have been proven to be safe, can be used. The capacity is from 0.02t to 30t, and the primary culture process has a capacity to select a structure suitable for the capacity such as abc of FIG. The larger one selects the type a and the smaller the capacity, the type c.

step. Select herbal medicine suitable for the purpose and extract hot water by boiling water. First, select the medicine suitable for the symptoms by considering the constitution and blood type, referring to (Table 1). Add 0.2g to 30g per liter of water. Water or water and chlorella water can be mixed or chlorella water can be added alone (ABC). The chlorella content of chlorella water is 0.002 to 3.0%. Fill the medicine with a filtering net or cotton in a medicine or water bath, and heat it at 62 ° to 200 ° C for 30 minutes to 2 hours and 50 minutes, and add the medicine and the liquid to the primary culture plant after filling 1/3 or more of the culture water. It is characterized by. The main medicine is garlic (Daesan). Tangerines. Casualty Injin (Han Injin). Gilkyung (bellflower). lacquer. Ginseng. Wild ginseng. Gold silver flower (Indongcho Flower). Ogapi jade porridge. Contrast (jujube). Seok Kwon-Baek (Rock Hand). Cheoncho (sancho). plum. Citron donkey. Rooting. Root root (knee bark bark). Morus peony. Crap. Chopi. Astragalus. Wormwood. Ginger Ginger. Pine tree. Tofu. Trilobite vinegar. Wolfberry. Kwonbaek (Buddha Son). Tenza. It is a soybean and the ingredients, efficacy and role of individual medicines can be referred to in Table 1. Role is △. □. ○ and △ are insecticides. Sterilization. Chlorella, such as deodorization, stabilizes the culture environment before it is fully activated and is thought to be an important primary mechanism in terms of metabolic engineering by aligning with the growth of chlorella.

□ is a secondary mechanism involving nutrients and growth processes required for chlorella cultivation and including prescriptions for constitution and symptoms. ○ Strengthened storage and distribution in preparation for shipments during the growth phase of chlorella. It is characterized by roles such as specific function enhancement (flavor, window complementary color). In addition, it is possible to add additional margin in selecting the material of herbal medicine. This chlorella culture method is to inject the active ingredients of herbal medicine as in Table 1-1,2,3,4,5 during the culturing process of chlorella to enhance the function of chlorella as a biocatalyst and combine with oriental medicine to constitution individually. bottle. blood type. It is possible to provide chlorella according to gender and the like. In particular, E. coli is commonly detected in conventional chlorella. Salmonella Staphylococcus aureus and the like to induce the sterilization of allicin in garlic to provide a safer chlorella. The roughness is more than 1000LUX and in the primary culture, the organic medium is added. Organic medium is also added in cotton cloth or 400 # strainer. Anchovy milk as organic medium. Anchovy. eel. Pollack milk. Emperor (ABC). It is characterized by using cod. After 1-3 days, filter out the sieve containing the medicine and organic matter, and also sieve the sieve of Chlorella. corona. Brain discharge. Electric discharge electric field. Irradiation. plasma. Ozone generator. UV lamp. Condensing using reflective mirrors or convex lenses. glare. It is characterized by injecting the ingredients of herbal medicine into the chlorella by conducting one or two at the same time or two or more at a time by shining a flame or boiling above 62 ℃. Incubate with aeration for 1 to 5 days, and the temperature should be 15 ℃ to 35 ℃ based on room temperature. When differentiation occurs under a microscope, transfer to the main culture center.

Table 1-1 Ingredients and Efficacy of Individual Herbal Medicines. Role in Chlorella Culture

Table 1-2 Ingredients and Efficacy of Individual Herbal Medicines. Role in Chlorella Culture

Table 1-3 Ingredients and Efficacy of Individual Herbal Medicines. Role in Chlorella Culture.

Table 1-4

Table 1-5

The stainless steel plate is used to make the primary, bone and shipping culture. Polycarbonate Woolite (White). Acrylic is used as bulkhead material. Place concrete on the place where you want to install it, train it for 3 ~ 5 days (winter 10 days), clean it with water and dry it well. Stainless steel pipe. The mold is made of plastics such as PVC. Bend round and weld or bolt two rings together. Connect by means of piece tightening, etc., and determine the height that fits the volume. The height of the bulkhead should not exceed 2m considering one piece.

Release the bulkhead material inside the mold to the same height as the finished mold. The ends overlap each other more than 15cm. In case of stainless steel, it is completed by welding with special welding rod. In case of plastic such as polycarbonate or woolite, follow the following.

`` A method of adhering the partition material to the mold and adhering in and out of the c-overline of Figure 8 with a small amount of resin (unsaturated polyester). Flat or round bolt head. One method selected from the method of fixing with a piece rivet or the like is carried out or simultaneously, and the gap is filled with resin to complete the appearance. Area d is ocher color where close attention is needed centering on the inlet and outlet. Area e green. Area f pink. Area blue is indicated as blue, which means that the closer the color is to red, the lower the risk of leakage. Fill the gap and keep the air tight by making FRP (Fiber-glass Reinforced plastic). In the manufacturing method, add fiber-glass or carbon fiber to unsaturated polyester (resin) and stir well using a stirring screw or rod. Safety glasses, mask) and hardener (methyl ethyl ketone peroxide). Summer is the ratio of Resin 100: Curing Agent 1 and Spring. Autumn is 100: 1.5 Winter is 100: 2 and curing time is 15 minutes in summer. spring. 20 minutes in autumn 30 minutes in winter. On the other hand, the curing catalyst (cobalt naphthenate) is a liquid and purple, which can be used in winter or in special cases. Particular attention should be paid to the curing agent (methyl ethyl ketone peroxide), which may cause an explosion. On the other hand, curing mitigators (hydroquinone, hardquinone mono ether 2-ethyl anthraquinone) can be used in summer. It is important to observe the amount of (Resin) 100: 0.005, and if it is too small to weigh on the balance, you can mix it in the ratio of Searchen Monobar 100: 10 and mix it with thinner. After complete preparation, the hardener should be added. After 20-30 minutes, the used containers should be immediately washed with a thinner and reused. The resin on the market is Aekyung Poricoat. Miwon Chemical Forista. Taekwang Chemical Formica. Uncetilated. Cree Valley etc. Grade for bathtub. It is suitable for artificial marble. The mixing ratio of fiber fiber or carbon fiber is (Resin) 70: (Binder) 30 or (Resin) 80: (Binder) 20 is good. Generally, when the film is thick, talc (TALC) is used. Because of its weakness and poor adhesion, aluminum hydroxide can be used as an alternative. In order to alleviate the difference in specific gravity of each raw material, 0.3 to 0.5% of aerosol (thixotropic airagent) is added. When using talc, the ratio of (resin) 60%: (talc) 25%: (binder) 15% is appropriate. Verrane for using mats where there are severe bends or where careful attention is required. Roving cross, and the like. Veran is suitable for uneven or three-dimensional areas, and roving fibers are suitable for gentle bends. ”The order of construction (Fig. 9) is in order d.e.f.g. The construction method is to remove the appropriate amount of resin with a small gourd or ladle, mix it well in proportion according to the season, referring to `` next '', and apply it evenly on the inside and bottom of the culture frame. When the first coat is like jelly, the mat is added to the place where the mat is needed. At this time, the air between the resin and the mat through the resin roller roll carefully.

Repeat this process two or three times and sand it when it is completely hardened. Scrubbers. Hitex. Use a sender or the like to finish the surface and wash off the straw. Boil the leaves 2 ~ 7 times with burned ash or fishery rainforest medicine. After filling the culture tank with water, the solution is exposed to direct sunlight for 1 to 7 days, and then a small amount of chlorella is added to the container.

단계. 본 배양장에서는 1∼10t의 배양수를 1∼5일전에 담아두어 직사광선 및 자외선에 노출시킨다 본 배양장(도 3의 가)지점이 되는데 미도시한 관로를 통해 펌핑한다 이동시간은 오전일 경우 8:30∼11:30분 오후에는 13:30∼17:30분 까지가 적당하다 이동이 완료 되었으면 30분∼3시간 안정시간을 준다 그 후로 아주 약하게 배양수를 회전시키거나 1000∼1500 배율 현미경 또는 전자 현미경으로 관찰하여 적응상황에 따라서 교반을 중지할 수 있다. 1∼7일이 지난후 실내온도 15°∼35°되고 오전에는 08:30∼11:30 오후에는 13:30∼17:30의 시간에 1∼7일 간격으로 1차식이를 공급한다 이 시기는 클로렐라가 본 배양장과 배양수. 온도 등의 주변환경에 적응하는 때이다 본 출원 클로렐라 성장 및 활동의 최고점은 오전10시 전후 오후3시 전후 인것을 특징으로 한다 1차식이는. 피로인산나트륨 일인산 소다. 이인산 소다 중에서 선택된 한가지와 빙초산을 혼합하여 CO2를 발생시키는 방법과 드라이 아이스를 0°∼30℃ 사이의 물에 녹이고 그 과정에서 나온 기체를 미도시된 관로를 통해서 교반라인에 투입하는 방법 그리고 액화탄산을 직접 미도시된 관로를 통해 교반라인에 투입하는 방법 중에서 한가지를 선택할 수 있다. 1차식이 및 배지로는. 중탄산암모니아. 일인산칼슘 또는 이인산 칼슘. 일인산 칼륨 또는 이인산 칼륨. 황산마그네슘(식용) 질소(식용). 과붕산소다(칼슘사용량의 6:1 또는 6:1 이하를 특징으로 한다) 결정 포도당. 녹반 등에 물을 첨가하여 30°∼220°까지 개별 용융점으로 가열하여 녹인 후 안전한 플라스틱 용기나 옹기 등에 배양수와 50∼100:1 전후의 비율로 섞고 집광 UV(1∼7일) 플라즈마. 초음파. 오존 발생기 중에서 한가지 또는 두가지를 동시에 실시하고 미도시관로로 이송하여 배양수의 수면에 비산 또는 분무한다.

step. In this culture, 1 ~ 10t of culture water is stored 1 ~ 5 days before exposure to direct sunlight and ultraviolet rays. This is the point of culture (a) of Fig. 3, but pumped through the pipe not shown. From 8:30 to 11:30 minutes in the afternoon, 13:30 to 17:30 minutes are suitable. If the movement is completed, give 30 minutes to 3 hours of rest time. Afterwards, rotate the culture water very weakly or use a 1000-1500 magnification microscope. Alternatively, the electron microscope can be observed to stop the stirring depending on the adaptation. After 1 to 7 days, the room temperature is 15 ° to 35 ° and in the morning 08: 30 ~ 11: 30 in the afternoon, 13: 30 ~ 17: 30 in the afternoon. Chlorella saw culture and cultured water. The peak of chlorella growth and activity of the present application is around 10 am and around 3 pm. Sodium pyrophosphate monosodium phosphate. Method of generating CO2 by mixing one selected from sodium phosphate and glacial acetic acid, dissolving dry ice in water between 0 ° ~ 30 ° C, and injecting the gas from the process into the stirring line through a not shown pipe line and liquefaction One of the methods of injecting carbonic acid directly into the stirring line through a pipe not shown may be selected. With primary diet and medium. Bicarbonate ammonia. Calcium monophosphate or diphosphate. Potassium monophosphate or potassium diphosphate. Magnesium sulfate (edible) nitrogen (edible). Sodium perborate (characterized by less than 6: 1 or 6: 1 of calcium use) Crystalline glucose. Water is added to the green spots, heated to individual melting points from 30 ° to 220 °, and melted, and then mixed with cultured water in a safe plastic container or pottery at a ratio of 50 to 100: 1 and condensed UV (1 to 7 days) plasma. ultrasonic wave. One or two of the ozone generators are carried out at the same time and transported to an unshown tube to be scattered or sprayed on the surface of the culture water.

The time is about 10 o'clock in the morning and around 3 o'clock in the afternoon. The second diet is NH ₄ Al (SO ₄ ) ₂ .12H ₂ O ammonium alum <(N ₄ ) ₂ SO ₄ Al ₂ (SO ₄ ) ₃ .24H ₂ O> .KAl (SO ₄ ) ₂ .12H ₂ O Soak one of the (ABC) in a sieve or strainer and soak in culture water and shake. The dosing time is effective from 8:30 am to 11:30 am and from 13:30 to 17:30 pm, with no additional defoamer added. When examined under a microscope, the differentiation is active, and the pH is 6 to 8.5. If the color turns to cyan or dark green, the culture water is increased. The spraying direction of the culture water is the opposite direction of rotation of the vortex. Increasing the amount of culture water is divided into three stages (in Fig. 3b) and is increased up to (b) in Fig. 3 to (b) in Fig. 3, the same as in (a) in Fig. 3). Generation is required, and the stirring motor is 0.2PS or more and continuously spray stirring using the nozzle.

Stirring time is 4 to 24 hours on a daily basis and varies with the seasons. Vortex came to be known in the 1950s when German victor Schauberg argued that it was the basic principle of moving the universe. For example, typhoons, tornadoes, and big bangs.

In the present application, the method of generating the vortex is arranged to displace the spray nozzles from the center of the 15 ° to 60 ° culture tank, and sprays the culture water onto the large culture water surface using the spray nozzle, supplies oxygen to the culture water surface, and supplies oxygen at the same time. It provides the rotational force of the culture water by using the spraying force. It generates vortex in the process of being sucked into the inlet of the lower part of the center of the culture tank, and the spraying force of the pump is added to the organic matter in the culture tank. The inorganic medium catalyst chlorella evenly spreads to enable a smooth culture. The material of the nozzle is stainless steel. PE. PVC. PC. PET. gold. platinum. Brass, etc. can be used

단계. 배양수를 미도시된 관로를 따라 출하 배양장으로 이동시켜 1∼7일간 숙성시키며 유통에 대비한 적응기간을 갖는다 이때 개별 체질에 맞는 한방 약재를 표 1-1∼5를 참고하여 혈액형. 병증에 맞는 처방을 첨가할 수 있다 출하배양장의 식이 공급은 중단되며 보텍스는 계속 발생시키되 점차적으로 회전력을 줄인다.If the volume of the plant is small, it is possible to generate vortex due to the pump's soil output without a nozzle. If the culture water is increased and reaches the point (Fig. 3), it is stirred for 1 to 7 days and the first and second diets are supplied. ～ 7.5, dark green and stable

step. The culture water is moved along the unillustrated pipeline to the shipping culture and matured for 1 to 7 days, and has an adaptation period for distribution. At this time, the herbal medicines suitable for the individual constitutions are referred to the blood types. It is possible to add the correct prescription for the condition. The dietary supply to the shipping site will be stopped and the vortex will continue to occur, but gradually reduce the rotational force.

단계를 출하 배양장이 아닌 본 배양장 (도 3의 다)지점에서 실시할 수도 있다. 본 출원 클로렐라 배양법의 특징을 본 배양장에서는 UV 또는 오존. 초음파 발생장치 등을 이용한 살균, 입자분쇄 등의 과정이 필요치 않은것이다.However, if the culture is small

The step may be carried out at the point of the main culture (C in FIG. 3), not the shipping culture. Characterized by the chlorella culture method of the present application is UV or ozone. Processes such as sterilization and particle grinding using ultrasonic generators are not necessary.

단계. PET. 캔. 병. 통 등에 50㎖. 500㎖. 1ℓ. 1.5ℓ 1.8ℓ. 4ℓ. 20ℓ. 파우치 등의 용기에 주입기를 통해 주입하고 포장.냉장저장 과정을 거쳐서 출하된다

step. PET. Cans. bottle. 50 ml in a barrel and the like. 500 ml. 1ℓ. 1.5 L 1.8 L. 4ℓ. 20ℓ. It is injected through the injector into a container such as a pouch and shipped through packaging and cold storage.

단계. 미도시된 관로를 따라 원심분리기로 이송하여 2500∼20,000rpm의 원심분리기로 분리하여 동결 건조하거나 엑기스. 분말. 과립. 원말형태로 가공하는것을 특징으로 한다 본 출원에서 클로렐라를 생촉매로 활용하여 한방의 유효 성분들을 모세혈관 끝단까지 보낼 수 있는 것은 첫째 크기가 2∼12㎛ 인것을 들수 있으며 이는 혈액속의 적혈구보다 작은 크기이다. 둘째 음료 형태이므로 마시는 순간부터 혀와 혀밑의 혈관과 식도 등 닿는곳의 대부분을 흡수투과 할수 있고 세포 내부로 클로렐라에 주입되어있는 한방성분을 전달할 수 있다. 많은 성분들이 이온화 되어 있으므로 이동이 원활한 것이 특징이다. 본 출원 클로렐라는 소양인으로 설정되어 있다 그런데 클로렐라는 도 7에서 처럼 반도체의 속성을 가지는데 섭취하는 사람의 신체 역시 근육이나 신경의 움직임을 본다면 전기적 흐름을 제어하여 근육을 움직이며 신경의 전달 역시 시넵스 되어 있으니 넓은 의미로 반도체의 속성을 가졌다고 할수 있다. 그러나 반도체는 상호간 함께 머무르는 시간의 한계성을 가지는데 20∼70시간으로 관찰되고 있다. 클로렐라가 흡수되어서 보상점에 다다르는 시간은 조건에 따른 차이가 있을 수 있지만 통상 4∼5시간으로 관찰되고 있다 따라서 본 출원 클로렐가 약리성분 전달. 체질개선. 등에서 더 효율적이라고 할수 있다. 세번째는 클로렐라가 미토콘드리아에게 많은 영향을 주는것으로 보고되고 있는데 이는 "산소 세상을 만든 분자 ; 닉레인/양은주옮김 2004"에서 주장하고 있는 클로렐라 등의 분류학적 구분에 의문을 제기하고 있는 것에 본 출원인 역시 공감하며 클로렐라의 생활환. 기능적패턴을 볼때 LUCA군에 속하는 고세균 등의 특징과 많은 유사점 들을 발견할 수 있다. 세포 내의 미토콘드리아와의 유사성으로 인해서 인체의 면역 반응을 완화시키거나 회피하면서 분자구조식 H₃O OH에서 볼수 있듯이 OH(히드록실라디컬)의 일차반응을 이용하여 체내의 문제점을 감지하고 라이너스 폴링의 주장처럼 "과량의 비타민"과 풍부한 수소(H)를 공급하여 일차적으로 미토콘드리아의 기능을 보조하고 점차 강화하며 손상된 미토콘드리아를 재생. 복제케하여 점진적으로 세포를 활성화시키는 것을 특징으로 한다. 또한 이상이 있는 세포의 내괴에 있는 철(Fe) 성분과 비타민의 반응을 유도하여 이상세포의 철(Fe) 성분을 제거함으로서 미토콘드리아와의 상호 작용으로 인해 정상 세포로 되돌리는 것을 특징으로 한다. 이러한 작용이 가능하도록 클로렐라가 기능을 수행하는 것이 본 출원의 또한가지 특징이다. 본 출원에 의하면 호전반응이 있을 수 있는데. 체질이나 신체의 이상 부위에 따라 다를수 있다. (도10)은 클로렐라 사이클을 나타내고 있으며(도11)은 호전반응 사이클을 나타낸 것이다. 일일 1.8ℓ의 클로렐라 배양수를 마시는 방법으로 적용되었으며 부위별 명현 현상은 표 2와 같다.

step. Transfer to a centrifuge along the pipeline not shown, separated by a centrifuge of 2500 ~ 20,000rpm freeze-dried or extracted. powder. Granules. In the present application, by using chlorella as a biocatalyst in this application, one of the active ingredients can be sent to the capillary end, and the first size is 2-12 μm, which is smaller than the red blood cells in the blood. to be. Secondly, since it is a beverage type, it can absorb and absorb most of the touching parts of the tongue, the blood vessels under the tongue, and the esophagus from the moment of drinking, and deliver the herbal ingredient injected into the chlorella into the cell. Since many components are ionized, the movement is smooth. The present application Chlorella is set as Soyangin But Chlorella has the properties of a semiconductor as shown in FIG. As a result, it can be said that it has the property of semiconductor in a broad sense. However, semiconductors have a limit of time to stay together, and are observed in 20 to 70 hours. Chlorella is absorbed to reach the point of compensation may vary depending on the condition, but usually 4 to 5 hours has been observed. Constitution improvement. More efficient on the back. Third, it is reported that chlorella has a lot of influence on mitochondria, which is why the applicant also sympathizes with the taxonomy of chlorella, which is claimed in the "molecules that created the oxygen world; Chlorella's life cycle. Looking at the functional patterns, many similarities can be found with the characteristics of archaea belonging to the LUCA family. The similarity with mitochondria in the cell helps to mitigate or evade the body's immune response, while using the primary reaction of hydroxyl radicals (OH) to detect problems in the body and claim Linus polling, as seen in the molecular structure H ₃ O OH. As such, it supplies "excess vitamin" and abundant hydrogen (H) to primarily support and gradually strengthen mitochondria and regenerate damaged mitochondria. It is characterized in that the cells are gradually activated by replication. In addition, by inducing the reaction of the iron (Fe) component and vitamin in the inner cell of the abnormal cell by removing the iron (Fe) component of the abnormal cell is characterized by returning to normal cells due to interaction with the mitochondria. It is another feature of the present application that the chlorella performs its function to enable this action. According to this application, there may be an improvement reaction. It may vary depending on the constitution or abnormal part of the body. FIG. 10 shows the chlorella cycle (FIG. 11) and the improvement cycle. It was applied by drinking 1.8 liters of chlorella culture water daily and the phenomena of each part are shown in Table 2.

<Table 2>

According to the present invention, it is possible to drink chlorella, thereby expanding its applicability, allowing the use of useful ingredients of herbal medicines inexpensively and in large quantities, and cultivating using vortex to enable more efficient cultivation. There is an advantage that can be applied to a variety of environmental health.

Claims (28)

As a method of culturing chlorella Ⅰ) Peel raw garlic, crush it in mortar, crush it, or chop it using a grinder. Ii) Method of eluting allicin by peeling raw garlic and soaking in 12.5-25% alcohol for 7-30 days. Ⅲ) Peeling raw garlic and baking it directly on a fire or baking it in a pan and Peeling and compressing raw garlic to produce fresh juice to induce allicin Simultaneously perform one or two selected from iii), ii), and iii) above to induce allicin by combining allinase and aliin, which were isolated in garlic, respectively. , Licorice in 0.2 ~ 30g weight per 1 liter of culture water wrapped in a strainer, cotton cloth, stainless steel mesh in 10 ~ 30 minutes into a primary culture of 2ℓ ~ 30,000ℓ capacity to spread allicin in the culture water containing more than 알리), ii), iii) by inducing allicin and 1Kg of Chlorella native per 50Kg of culture water wrapped in stainless steel, cotton cloth, strainer, etc., and are safe from Staphylococcus aureus, Escherichia coli, Helicobacter pylori, Salmonella A) a method of heating the mixed solution at 62 to 65 ° for 1 hour b) a method of heating at 62 ° to 65 ° for 30 minutes c) at 85 to 90 ° ① 10 seconds How to heat ② How to heat 20 seconds to 30 seconds ③ How to heat 1 to 5 minutes, ④ How to heat 10 to 30 minutes ⑤ How to heat 1 hour to 2 hours 50 minutes d) 30 seconds to 92 ° to 95 ° Boiling method e) 92 ° to 95 ° 10 minutes boiling method, f) 92 ° to 95 ° 1 hour to 2 hours 50 minutes boiling method is carried out at the same time one or two selected by PCR (polymerase chain reaction) characterized in that To cultivate chlorella The herbal medicine according to claim 1, wherein the herbal medicines include garlic, tangerine, casualties, injin, Gilkyung, lacquer, ginseng, gold and silver, jade, control, sancho, donkey, brown root, root roots, baekbaekpi, flatul, astragalus, ginkgo, ginger, tofu, Method for culturing chlorella, characterized by injecting trichophytic vinegar, wolfberry, soybean ingredients into the primary culture The method of claim 1, wherein the herbal medicines garlic, tangerine, casualties, jinjin, Gilkyung, lacquer, soybean, wild ginseng, gold and silver, jade, control, seokkwonbaek, plum, citron, brown root, root skin, baekbaekpi, hut, chopi, astragalus Method of culturing chlorella, characterized by injecting mugwort, ginger, pine needles, larvae, trichophytic vinegar, wolfberry, tanza ingredients into the primary culture The method of claim 1, wherein the herbal medicines garlic, casualties, injin, gilyeong, ginseng, gold and silver coins, ginseng, jade porridge, contrast, sancho, plum, yuzu, donkey, brown root, root root, baekbaekpi, hut, Astragalus, mugwort, ginkgo, Method of culturing chlorella, characterized by injecting the worms, trigeminal vinegar, gojija, tangja, soybean ingredients in the primary culture The method of claim 1, wherein the herbal medicines, garlic, tangerine peel, casualties, jinjin, Gilkyung, gilding, jade, control, Angelica, Yugeunpi, hut, chopi, Astragalus, wolfberry, soybean ingredients are injected into the primary culture To cultivate chlorella [Claim 2] The chlorella according to claim 1, wherein the herbal medicines of garlic, casualties, injin, gilyeong, ogapi, jade, control, citron, brown root, hut, chopi, trichophyllum and soybean are injected into the primary culture. How to incubate. The method of claim 1, wherein the herbal medicine, garlic, tangerine, casualties, jinjin, gilyeong, ginseng, control, sancho, Angelica, Yugeunpi, baekbaekpi, hut, Astragalus, ginger, trigeminal vinegar, soybean ingredients in the primary culture Method of culturing chlorella, characterized in that the injection According to claim 1, wherein the herbal medicines garlic, casualties, injin, Gilkyung, lacquer, ginseng, gold silver, jade porridge, contrast, stone Kwonbaek, citron, donkey, brown root, root roots, barn, chopi, Astragalus, ginger, soybean Method for culturing chlorella, characterized in that the injection into the primary culture The method of claim 1, wherein the herbal medicine, garlic, casualties, jinjin, gilyeong, lacquer, sterling silver, organza, jade porridge, control, citron, donkey, baekbaekpi, hut, mugwort, ginkgo, trifolium vinegar, wolfberry, soybean Method for culturing chlorella, characterized in that the injection into the tea culture The method of claim 1, wherein the herbs, garlic, tangerine, casualties, injin, Gilkyung, wild ginseng, gold and silver, control, Angelica, brown root, root roots, baekbaekpi, chopi, hut, mugwort, ginkgo, trigeminal vinegar, soybean Method for culturing chlorella, characterized in that the injection into the culture The method of claim 1, wherein the herbal medicines garlic, tangerine peel, casualties, injin, lacquer, ginseng, jade porridge, control, sancho, brown root, root root, baekbaekpi, Astragalus, ginkgo, tofu, wolfberry, tanza ingredients in the primary culture Method of culturing chlorella, characterized in that the injection [Claim 2] The method of culturing chlorella according to claim 1, wherein the herbal medicine is injecting garlic, gilyeong, casualty, injin, jade porridge, control, root skin, sancho, hut and ginger into the primary culture. In the method of culturing chlorella Anchovy, eel, yellow, cod, pollock, organic medium, were extracted by hot water extraction at temperatures ranging from 92 ° to 200 °, or extracted by boiling with ABC (chlorella culture alone mixed with organic medium). Anchovy lacquer, pollack, and shrimp lacquer are wrapped in net, etc., and wrapped in stainless steel, cotton cloth, manure net, etc. in an amount of 0.000066% to 0.00033% of total culture water (water) In addition, one or two or more organic mediums are added to the primary culture at the time of adding herbal medicines such as licorice, jinjin, casualty and sancho, or after extracting the herbal medicines, and the amount of sunshine due to extreme weather during chlorella culture It also supplies nutrients that help the activity of chlorella by injecting it even when the chlorella growth force is weakened due to insufficient or abnormal high temperature. To cultivate chlorella In the method of culturing chlorella, PCR is performed by laser, corona, brain discharge, electric discharge, radiation, plasma, ozone generator, UV lamp, condensing by reflecting mirror, glare, flame, condensing by lens, and water. (Polymerase chain reaction) to be injected into the shaking culture medium of the primary culture, 1 to 20 minutes 2) 1:10 minutes 3) 1:30 minutes 4) 2 hours, heating between 62 ~ 65 ° A) 20 seconds b) 2 minutes c) 3 minutes d) 5 minutes, e) 8 minutes f) 30 minutes boiling and A) 15 seconds, B) at 92-95 ° 40 to 50 seconds, C) 3 minutes, D) 6 to 8 minutes, E) 50 minutes Boiling method is carried out simultaneously with one or two selected methods and two or more times at different times to PCR (polymerase chain reaction) Herbal ingredients garlic, tangerine, casualties, injin, gilkyung, lacquer, ginseng, wild ginseng, gold and silver, ogapi, jade porridge, contrast, scallop, sancho, plum, citron, donkey, brown root, Root, lettuce, hut, chopi, Astragalus, Wormwood, Ginkgo, Ginger, Beef, Peel, Trichophyte, Trichophytium, Gojija, Kwonbaek, Tenza, Soybean, Licorice Ingredients per liter of water Take a weight of the selected one and dissolve in alcohol of the herbal medicine ingredient method of culturing chlorella, characterized in that 50 to 120g of mixing In the differentiation of chlorella by semi-continuous culture method, laser, corona, brain discharge, electric discharge, radiation, plasma, ozone generator, UV lamp, condensing by reflecting mirror, glare, flame, condensing by convex lens, and heating water PCR is carried out by the method and injected into the shake culture medium of the primary culture, and 1) 20 minutes 2) 30 minutes 3) 1 hour 4) 1 hour 10 minutes 5 1) 30 minutes 6) 2 hours heating and 85 ° C to 91 ° A) 15 seconds B) 40 to 50 seconds C) 3 minutes D) 6 to 8 minutes E) 50 minutes to boil 92 to 95 ° Under the conditions a) 20 seconds b) 30 seconds c) 60 seconds d) 2 minutes e) 3 minutes f) 5 minutes g) 8 to 30 minutes Boiling is carried out simultaneously with one or two of two or more PCR (Polymerase Chain Reaction) is carried out with time difference and the herbal ingredients garlic, tangerine, casualty, injin, gilkyung, lacquer, ginseng, wild ginseng, gold silver flower, ogapi, jade porridge , Contrasting, Tekkenbaek, Sancho, Plum, Citron, Angelica, Brown root, Root of root, Morus, Bark, Chopi, Astragalus, Mugwort, Ginkgo, Ginger, Pine needle, Sesame, Trifolium vinegar, Wolfberry, Rolled white, Tenza, Soybean, Licorice Chlorella is differentiated by mixing 50-120 g of one weight selected from 0.2 to 10 g or 11 g to 20 g of Chinese herbal medicine dry weight per 1 liter of water, and dissolving it in alcohol as an ingredient of herbal medicine. Method for culturing chlorella, characterized in that the cycle has a cycle of 4 to 6 months starting from 20 days. In the method of culturing chlorella 1) Hot liquor extraction or ABC (chlorella) is carried out in a primary culture plant with a volume of 2 L to 30,000 L at a weight of 0.2 to 30 g per 1 L of culture water. Boiled by mixing with herbal medicine alone in a culture solution), wrapped in cotton or strainer, stented net and soaked in herbal medicine, raw chlorella is also put in a strainer and aerated for 1-7 days, and the organic matter medium at 15-35 ° conditions. After 1 ~ 3 days of culturing, add medicinal herbs, chlorella, and sieves containing organic medium, and then irradiate with plasma, laser irradiation, ozone, or UV lamp, or boil or heat water to bring pharmacological ingredients into chlorella. PCR (Polymerase Chain Reaction) step 2) Incubate with aeration for 1 ~ 5 days and on the other hand, 1 ~ 10t of purified water or pear Step to put a number of 1-7 days exposure to sunlight or ultraviolet light, and 3) increase the amount 1000-1500 viewed with magnification optical microscope or an electron microscope such as phase transfer activity in the culture section is shown being differentiated in some eases, 4) In this culture, if the culture water is weakly rotated or growth is good, leave it as it is and after 1-7 days, the culture temperature is 15 ° to 35 °, and in the morning 08: 30 ~ 11: 30 minutes 13: Feeding the first diet at 30-17: 30, 5) In the first to fifth dietary feeds, turn green or dark green, and have a pH of 6 to 8.5. If active differentiation occurs under a microscope, supply additional culture water and spray or spray very weakly when supplying the second culture water. After operating the stirring motor pump to generate the vortex, observe the state of chlorella within 2 to 84 hours after the culture water reached half the height of the culture center. If active differentiation is seen in the step of additionally adding culture water, 6) After adding the culture water, continue to generate vortex and operate the stirring motor pump. When the height of the culture water reaches half point at half of the culture center, stir for 1 to 7 days, and at the appropriate time, the 1st to 5th diet After supplying and inducing proliferation to move to the shipping culture to generate a vortex for a certain period (within 30 days) and maintaining the release status of chlorella, the method of culturing chlorella. In the process of generating the vortex in the process of culturing chlorella, the specific method is to set the vortex axis to 90 ° in the primary culture, the main culture, and the delivery culture, and then select 46.7 in either of clockwise or counterclockwise directions. If the number of cultures is smaller than 본 of this culture center or the temperature is lower than 4 ℃, adjust the spray nozzle angle in the direction of 60 ° close to the vortex axis to slow down the rotation speed of the vortex, and the culture water is close to the ⅔ point of this culture center. If the active or different chlorella differentiation is occurring due to proper or different chlorella differentiation conditions, the spray nozzle angle is adjusted in 15 ° direction to increase the efficiency of chlorella cultivation and the slope of the bottom of the plant is characterized by 0-20 °. The proper ratio of the culture water / pump is made of chlorella characterized by two 30t / 0.3 ~ 0.5HP or one 30t / 0.7 ~ 1HP How to As a method of generating carbon dioxide required in the chlorella cultivation process, dilute glacial acetic acid with one selected from sodium pyrophosphate, sodium monophosphate, and sodium diphosphate. Sodium pyrophosphate is heated at 99 ° to 110 ° C. for 4 hours. After dissolving and melting, it is dissolved again in water by 20% weight, reheated to 98 ° ~ 110 ° C, and added by dropping glacial acetic acid until it reaches PH 6.5. Sodium pyrophosphate 100g: ratio of 5-10g glacial acetic acid Method for culturing chlorella, characterized in that the solution is added to about 0.0002667% of the total chlorella culture water As a primary dietary and inorganic medium in the method of culturing chlorella, ammonia bicarbonate, calcium monophosphate or calcium diphosphate, potassium monophosphate or potassium diphosphate, crystal glucose, magnesium sulfate (edible), nitrogen (edible), green plate, Sodium perborate (6: 1 or 6: 1 or less of calcium monophosphate or calcium diphosphate used) is dissolved at the melting point of the individual medicines in a ratio of 1 to 2 of inorganic medium 1: water, and then dissolved again in 50 to water. Diluting at a magnification of 100, one or two selected from among condensing, UV, plasma, ultrasonic, and ozone generators are simultaneously carried out and stabilized, followed by culturing chlorella, characterized in that it is added to the culture. In the method of culturing chlorella, the first to fifth dietary input and the addition of culture water (increase) during the cultivation of chlorella by an outdoor cultivation method using natural light and an eclectic cultivation method (increase) around 10 am or 3:00 pm Method for culturing chlorella, characterized in that In the method of culturing chlorella, NH ₄ Al (SO ₄ ) _2, 12H ₂ O, (N ₄ ) ₂ SO _4, Al ₂ (SO ₄ ) ₃ 24H ₂ O, KAl (SO ₄ ) _2, 12H ₂ O chlorella immersed in a sieve or stainless steel, immersed in chlorella culture water and shaking the method of culturing chlorella characterized in that it is put into the culture In culturing chlorella, ammonia bicarbonate, calcium monophosphate or calcium diphosphate, potassium monophosphate or potassium diphosphate, which are cultured or primary dietary and inorganic mediums in the opposite direction of rotation of vortex when the chlorella culture water is increased in this culture, Crystalline glucose, magnesium sulfate (edible), nitrogen (edible), sodium perborate (6: 1 or 6: 1 or less of calcium monophosphate or calcium diphosphate), green plate, etc. After dissolving by heating to the melting point of the individual medicinal herbs and diluting the water to 50 ~ 100 times again one or two selected from condensing, UV, plasma, ultrasonic, ozone generator at the same time and stabilized the main mixture solution A method of culturing chlorella, characterized by setting the vortex axis of the intestine to 90 ° and scattering or spraying the spray nozzle at 46.7 ° to 90 °. delete In culturing chlorella, it is cultured in the order of primary culture, main culture, and shipping culture, and the temperature of the shipping culture is kept at room temperature or 3 to 4 ° to generate a stationary state or vortex, and keep it for 5 to 30 days. Method for culturing chlorella, characterized in that to increase the delete delete delete The method according to any one of claims 1 to 15, 17, 19, 21, 22 and 24, wherein LUCA <Luaclast universal Ancestor and LCA (Last Common Ancestor) are combined, Photosynthetic bacteria including Rhodopseu do monas sphaeroides, Rhodopse do monas blastica, Rhodoseu do monas palustris, Rhodo cyclus gelationsus, and Rhodo cyclus tenuis Hydrogen-producing microorganisms, including spirulina, euglenozoa, euglena, nullsulfbacteria, red tide-responsive microorganisms including Hahelezogenensis, vulgaris, Bacillus, Rhodo-spirillum rubum, Green sulf bacteria Purple one or two selected from sulfur bacteria, red bacteria including Dinococcus radio Durans alpha proteo bacteria, cyano bacteria, Rhodop seud monas rutild Culture methods characterized in that the culture at the time.

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