KR100963227B1 - Composition containing fermented extract of medicinal plant for prevention or relief of alcoholic hangover and manufacturing method thereof - Google Patents

Abstract

PURPOSE: A composition containing medicinal herb extrct fermentation is provided to suppress alcohol dehydrogenase(ADH) activation and to promote aldehyde dehydrogenase](ALDH) activation. CONSTITUTION: A composition for preenting or relieving hangover contains medicinal herb extract fermentation as an active ingredient. A method for manufacturing the fermentation comprises: a step of extracting Glycyrrhiza uralensis, Radix pueraria, Lycium chinensis, Liriope platyphylla, Polypori umbellati Polyporaceae, Ixeris dentate, Acanthopanax sessiliflorum, Artemisia capillaris Thunb, Paeonia lactiflora Pallas, Hovenia dulcis Thunb, Plantago asiatica L., or Panax ginseng(red) using water, alcohol of C1-C3 or mixture thereof; a step of decompressing the mixture; a step of adding Aspergillus oryzae to perform saccharification; and a step of fermenting with lactobacillus.

Description

Composition containing fermented extract of medicinal plant for prevention or relief of alcoholic hangover and manufacturing method according to medicinal plant extract fermentation as an active ingredient

The present invention relates to a composition for preventing or eliminating hangovers containing medicinal plant extract fermentation as an active ingredient, and more particularly, to inhibiting alcohol dehydrogenase (ADH) activity without adverse effects on the human body. While promoting the activity of aldehyde dehydrogenase (Aldehyde dehydrogenase, ALDH) to hangover prevention or hangover prevention and remedy composition and manufacturing method containing the medicinal plant extract fermentation with excellent effect as an active ingredient.

Although alcohol consumption is increasing rapidly to relieve stress of modern people, symptoms such as nausea, vomiting, dizziness, thirst, lethargy, headache and muscle pain caused by acute alcoholic hangover are causing socioeconomic damages due to decreased work efficiency. In chronic alcohol intake, pancreatitis, myocardial infarction, neurological disorders, tuberculosis, and other disorders appear, leading to fatal damage to liver structure and function.

Alcohol is rapidly metabolized to acetaldehyde by the liver by alcohol dehydrogenase (ADH), microsomal ethanol oxidizing system (MEOS) and perocidase, rather than the effect of alcohol on the liver. It is known that the effect of acetaldehyde, an alcohol metabolite, is greater.

Meanwhile, acetaldehyde is oxidized to acetic acid by aldehyde dehydrogenase (ALDH) to prevent acetaldehyde accumulation and its side effects. To this end, it is necessary to examine the effects of aldehyde dehydrogenase (ALDH) together with alcohol dehydrogenase (ADH).

Recently, drugs for reducing and eliminating hangovers after drinking have been developed, but due to their toxicity or side effects, much attention has been drawn to the development of safer natural-derived health drinks.

Accordingly, there is a trend of increasing patent applications for hangover composition using natural plant extracts.

Korean Unexamined Patent Publication No. 10-2006-0017559 discloses a hangover tea using wild ginseng culture root, dried persimmon, brown root, Schisandra chinensis, ginger extract,

Republic of Korea Patent Publication No. 10-2006-0095038 discloses a drink composition for hangover elimination using the extract of cedar mugwort, moisture grass, root vegetables, old root, seedlings,

Korean Unexamined Patent Publication No. 10-2007-0101443 discloses a hangover composition using the bark fruit, Injinho, brown root, dermis, creation, licorice extract.

Applicant of the present invention has completed the present invention by confirming that the hangover relief effect of the medicinal plant extract fermentation fermented medicinal plant extract is greater than the simple extract of the medicinal plant.

It is an object of the present invention to inhibit ethanol dehydrogenase (ADH) activity while promoting the aldehyde dehydrogenase (ALDH) activity while inhibiting the alcohol dehydrogenase (ADH) activity active ingredient extract medicinal plants excellent in hangover It is to provide a composition and manufacturing method for preventing or eliminating hangover.

In order to achieve the above object, the present invention provides the following solution.

The present invention water medicinal plants that are effective in relieving hangover, C ₁ -C ₃ Extracting with alcohol or a mixture thereof and then concentrating under reduced pressure to prepare an extract (step 1);

Adding a bacterium-containing composition to the extract to obtain a saccharified extract through a saccharification process (step 2); And

It provides a composition for preventing or relieving hangover containing the medicinal plant extract fermented product prepared by the manufacturing method comprising the step of fermenting the saccharified extract with lactic acid bacteria (step 3).

The medicinal plants of step 1 can be used without limiting the medicinal plants are effective in relieving hangover, preferably licorice, brown root, gojija, Mcmun-dong, Baekbongryeong, Soksi, Ogapi, Injin mugwort, Peony, earth, chajeoncho and red ginseng It is preferable to include one or more selected from the group consisting of.

Extraction of the step 1 may use a known extraction method, the extraction solvent is water, C ₁ -C ₃ Alcohols or mixtures thereof can be used, preferably water is used as the extraction solvent.

In the extraction, the extraction conditions are not particularly limited, and a general water bath method can be used. When extracting using water as a solvent, there is no particular limitation on the amount of water, but considering the extraction temperature and time, 5 to 40 times the weight of the medicinal plant can be used, and the extraction conditions are 12 to 12 at a temperature of 80 to 100 ℃. It can be extracted for 72 hours.

The saccharification process of step 2 may be added 1 to 10 parts by weight with respect to 100 parts by weight of the Nuruk, it may be carried out at 15 to 55 ℃ for 36 to 72 hours.

Fermentation of step 3 may be carried out at 15 to 55 ℃ for 1 to 10 days.

In addition, in order to achieve the above object, the present invention

Extracting a medicinal plant effective in relieving hangover with water, C ₁ -C ₃ alcohol or a mixture thereof, and then concentrating under reduced pressure to prepare an extract (step 1);

Adding a bacterium-containing composition to the extract to obtain a saccharified extract through a saccharification process (step 2);

Fermenting the saccharified extract with lactic acid bacteria to obtain a fermentation broth (step 3);

It provides a method of producing a medicinal plant extract fermentation broth comprising a.

The composition according to the present invention can be used as it is without a separate excipient, in which case it is also possible to dilute and ingest the stock solution as water. On the other hand, the composition according to the present invention can be prepared in various forms, such as beverages, drops in the form of powder, tablets, etc. It is also possible to prepare the addition of the fermented product to general food. In the case of the manufacture of the ring can be prepared only with fermentation without excipients, it can also be prepared with various additives.

Hangover prevention or remedy composition containing medicinal plant extract fermentation according to the present invention as an active ingredient has no side effects on the human body, while inhibiting the alcohol dehydrogenase (Alcohol dehydrogenase, ADH) activity while aldehyde dehydrogenase (Aldehyde dehydrogenase, ALDH) It has the effect of promoting activity.

Thus, by reducing the concentration of acetaldehyde in the blood known as a major cause of hangover, there is an effect that can reduce the harmful physical and mental symptoms appearing after alcohol consumption. On the other hand, in the case of a hangover prevention or remedy composition containing the medicinal plant extract fermentation according to the present invention as an active ingredient, there is an effect of improving the taste in the taste through fermentation.

Hereinafter, the present invention will be described in detail.

The hangover relief or prevention composition according to the present invention will be described.

Hangover relief or prophylactic composition according to the present invention contains a medicinal plant extract fermented product prepared according to the following manufacturing method as an active ingredient.

The medicinal plant extract fermented medicinal plants that are effective in relieving hangovers, C ₁ -C ₃ Extracting with alcohol or a mixture thereof and then concentrating under reduced pressure to prepare an extract (step 1);

Adding a bacterium-containing composition to the extract to obtain a saccharified extract through a saccharification process (step 2); And

Fermenting the saccharified extract with lactic acid bacteria can be prepared by a manufacturing method comprising the step (step 3) to obtain a fermentation broth.

Hereinafter, step 1 will be described in detail.

The medicinal plants that are effective in relieving hangovers can be used without limitation for all medicinal plants that are effective in relieving hangover, including medicinal plants that are known to be effective in relieving hangovers. The medicinal plants preferably include one or more selected from the group consisting of licorice, brown root, gojija, Mcmundong, Baekbongyeong, genus, Ogapi, jinjin mugwort, peony, earth, chajeoncho and red ginseng. When the medicinal plants include licorice, brown root, wolfberry, macmundong, baekbongryeong, genus, Ogapi, jinjin mugwort, peony, earth, chajeoncho and red ginseng, the medicinal plants of the step 1 is licorice 1 to 10 parts by weight, gal root 10 To 30 parts by weight, wolfberry 5 to 15 parts by weight, 1 to 10 parts by weight of Macmundong, 1 to 10 parts by weight of Baeknyeongyeong, 5 to 15 parts by weight, 5 to 15 parts by weight of Ogapi, 5 to 15 parts by weight of jinjin mugwort, 1 to about peony It is preferable to include 10 parts by weight, earth 1 to 10 parts by weight, 1 to 10 parts by weight of chajeoncho and 1 to 20 parts by weight of red ginseng.

The composition ratio was derived through ADH and ALDH experiments to find the optimal composition ratio that can maximize the alcoholase activity. On the other hand, the medicinal plants can be purchased through herbal medicines, when used directly in nature, licorice roots, roots roots, roots of goji berries, fruit berries, makmundong roots, roots of Baekbongryeong, roots of the inner ear, the skin of the Ogapi, Injin mugwort Silver stems, leaves, or a mixture thereof, peony roots, earthly fruit, chajeoncho whole, red ginseng root.

Extraction of medicinal plants in step 1 is made according to a conventional extraction method, the extraction solvent is preferably water. Extraction conditions are not particularly limited and general extraction conditions may be arbitrarily selected by those skilled in the art, for example, extraction at a temperature of 80 to 100 ℃ for 12 to 72 hours. When extracted using water, for example, it can be extracted using 5 to 40 times the weight of medicinal plants. In the extracting step of step 1, the extract itself may be used as it is in the next step, but the extract may be used in the next step, which has been concentrated through a general vacuum concentration. Concentration has the advantage of reducing the volume of the reactor used in the subsequent fermentation process. In case of concentration, the concentration is appropriately concentrated to a concentration of 2.5 to 15 ° Bx (Brix).

Hereinafter, step 2 will be described in detail in a method of preparing extract fermentation of medicinal plants. Step 2 is a step of fermentation and saccharification of the extract obtained in step 1 with a composition containing the bacterium. This step is to produce monosaccharides by saccharification and to induce lactic acid bacteria fermentation in the next step more easily by such monosaccharides. The saccharification process according to this step is optional in the extract fermentation method of the present invention, and thus may be passed to the lactic acid bacterium fermentation process of step 3 except for step 2. This saccharification process is to help fermentation of polysaccharides such as starch in medicinal plants.

The composition containing the bacterium used in the saccharification process of step 2 is preferably a composition containing yeast mold, and can be used as a conventional yeast. The amount of the composition containing the bacterium used is not particularly limited, but may be added in an amount of 1 to 10 parts by weight based on 100 parts by weight of the extract prepared in step 1, and saccharification conditions are not particularly limited, but 12 hours at a temperature of 15 to 55 ° C. It can be carried out for the above, preferably, 1 to 5 parts by weight of the bacterium-containing composition is added. The content of reducing sugar in the saccharified extract increases with the saccharification time and shows a constant reducing sugar concentration over 36 hours. On the other hand, when saccharifying for 60 hours or more, the content of sugar may decrease, and fermentation for longer time is not meaningful, so saccharification is appropriately performed in the range of 36 hours to 60 hours.

Step 3 is a process of lactic acid fermentation of the extract obtained in step 1 or the glycated extract obtained in step 2. The lactic acid bacteria used in this step may be any bacteria that produce lactic acid, which is generally referred to as lactic acid bacteria, and lactic acid bacteria such as Lactobacillus, Streptococcus, Pediococcus, Leukonostock or Bifidobacterium genus. Can be used. Specifically Lac . bulgaricus , Strepto . lactis , Bifido . bifidum , Leuc . lactis , Lac . delbruechii , Leuc . mesenteroides Strains such as can be used. Fermentation conditions of this step is not particularly limited, but may be carried out for 1 to 15 days at a temperature of 15 to 55 ℃ as an example. The fermentation period may preferably be carried out for 1 to 10 days, more preferably for 2 to 10 days. The content of lactic acid bacteria used in this step may be appropriately selected and adjusted by those skilled in the art.

The present invention will be described through the following examples. This embodiment is only one example for aiding the understanding of the present invention, and the scope of the present invention should not be unduly limited by the present embodiment.

Example  1-6. Medicinal Plant Extracts Fermented product  Produce

Step 1. Preparation of medicinal plant extract

Licorice 5g, 20g root, 20g wolfberry 10g, Mcmundong 5g, Baekbongryeong 5g, 10g ginseng, 10g, 10g ginseng, ginseng 10g, peony 5g, earthen 5g, chajeoncho 5g and red ginseng 10g, 20g weight of 100g mixture After extraction for 48 hours at a temperature of 90 ℃, and concentrated under reduced pressure so that the final extract is 10 ° Bx to prepare an extract to be used in the next step.

Step 2. Glycosylated  Preparation of Extract

To 100 g of the medicinal plant extract of step 1, 3 g of improved Nuruk (Gyeongin Pharmaceutical Co., Ltd. No. 29) manufactured by Junggokja (Co., Ltd., Chungju) was added and saccharified at 35 ° C for 72 hours.

Step 3. Fermented product  Produce

Six kinds of microorganisms used for fermentation of medicinal plant extracts were selected and inoculated and cultured in MRS broth medium, and lactic acid fermentation was performed on the glycosylated extracts subjected to Step 2. Six microorganisms selected are shown in Table 1 below.

Table 1.

division microbe Example 1 Lactobacillus bulgaricus KCTC 3188 Example 2 Streptococcus lactis KCTC 3191 Example 3 Bifidobacterium bifidum KCTC 3202 Example 4 Leuconostoc lactis KCTC 3528 Example 5 Lactobacillus delbruechii sub. sp. lactis KCTC3636 Example 6 Leuconostoc mesenteroides KCTC 3718

Fermentation was all done for 10 days at 35 ℃, to obtain a fermentation broth using each strain.

Experimental Example  1. Alcohol dehydrogenase against 25 medicinal plants ADH ) And Aldehyde  Dehydrogenase ( ALDH Activity experiment

Twelve medicinal plants of red ginseng and less than the licorice used in the examples were examined by the latest herbal medicine, the herbal medicine of clinical herbal medicine, and then selected 25 kinds of medicinal plants known to be antioxidant and inhibitory to alcohol dehydrogenase, Screening was carried out through alcohol dehydrogenase and aldehyde dehydrogenase activity experiments.

The 25 selected medicinal plants are shown in Table 2 below.

Table 2.

division Scientific Name Use area licorice Glycyrrhiza uralensis Root Rooting Radix pueraria Root Wolfberry Lycium chinensis Fruit Geumchocho Glechoma hederacea Linne var. grandis Kudo Stem, leaf Donkey Angelica gigas Nakai Root Tofu Eucommia ulmoides Oliver Bark Macmundong Liriope platyphylla Root Baekbongryeong Polypori umbellati Polyporaceae Root Whiteness Atractylodes japonic Root Medicine Dioscorea Batatas Decaisne Root Three hundred seconds Saururus chinensis BAILL Root Inner bird Ixeris dentata Root Hungjiwang Rehmannia glutinosa Root Ogapi Acanthopanax sessiliflorum coat Root skin Ulmus macrocarpa Hance Bark Indong Lonicera japonica Thunberg Stem, leaf Injin mugwort Artemisia capillaris Thunb Stem, leaf Peony Paeonia lactiflora Pallas Root Earth Hovenia dulcis Thunb Fruit Chachocho Plantago asiatica L. all Cheongung Ligusticum officinale Kitag Root Tobok Spirit Smilax china linne Root The beginning Geranium nepalense subsp. thunbergii leaf Red ginseng Panax ginseng (red) Root Black bean Glycine max Fruit

25 kinds of medicinal plants in Table 2 were pulverized into 70mesh particles and used as experimental materials.

20 g of distilled water was added to 10 g of each dry powder, and a reflux condenser was attached in a water bath at 80-90 ° C. for 5 to 7 hours. The filtrate was filtered and concentrated under reduced pressure. Used as a sample.

The alcohol degrading enzyme activity of the 25 medicinal plant extracts was conducted.

ADH (Sigma A7011-30KU), ALDH (Sigma 82884) and NAD + (Sigma 43407) were purchased from Sigma (Sigma chemical, co., USA) and used.

ADH  Activity measurement

ADH activity was measured by measuring the absorbance of NADH formed at 340nm using a spectrophotometer to indicate the activity. That is, 0.1 ml of ethanol, 0.5 ml of NAD solution (2 mg / ml) and 0.1 ml of each medicinal plant extract were added to the test tube, and 0.01 M glycine-NaOH buffer solution (pH 8.8) was added so that the total volume was 1.8 ml. The reaction was carried out in a 25 ° C. thermostat for 10 minutes, and 0.25 ml of ADH (18 units / ml) was added to measure the change in absorbance at 340 nm. In this case, 0.25 ml of 0.01 M glycine-NaOH buffer solution was used instead of ADH. In the activity of ADH, the maximum absorbance at the end of the reaction was determined relative to the maximum absorbance of the control (%), the results are shown in Table 3.

ALDH  Activity measurement

The activity of ALDH was measured at 340 nm in the change of absorbance according to NADH production. To measure the activity of ALDH, 0.1 ml of ALDH (1 unit / ml) and 0.1 ml of medicinal plant extract were added to 2.25 ml of 50 mM sodium pyrophosphate buffer solution (pH 8.8), 0.5 mM NAD, 0.1 mM pyrazole, and 5 mM acetaldehyde. After the addition, the mixture was left for 10 minutes in a 25 ° C. thermostat and absorbance was measured at 340 nm. The activity of ALDH was expressed by the same method as that of ADH, and the results are shown in Table 3.

Table 3.

division Activity (%) ADH ALDH licorice -0.3 112.3 Rooting -3.2 147.4 Wolfberry -1.7 163.8 Geumchocho 52.4 88.2 Donkey 74.3 102.3 Tofu 67.1 127.37 Macmundong 4.8 143.2 Baekbongryeong 10.2 152.0 Whiteness 79.2 92.4 Medicine 82.6 102.6 Three hundred seconds 88.2 79.6 Inner bird 8.3 154.1 Hungjiwang 76.8 117.2 Ogapi -3.5 166.3 Root skin 25.7 90.8 Indong 44.2 120.8 Injin mugwort 10.9 126.4 Peony 11.2 129.5 Earth 13.1 138.6 Chachocho 41.2 115.4 Cheongung -2.1 140.0 Tobok Spirit 63.5 114.9 The beginning 76.3 120.2 Red ginseng 1.6 128.5 Black bean 42.5 136.3

According to the experiment, 12 medicinal plants with high ADH inhibitory activity and high ALDH promoting activity were selected and shown in Table 4.

Table 4.

Medicinal Plants Use area One licorice Root 2 Rooting Root 3 Wolfberry Fruit 4 Macmundong Root 5 Baekbongryeong Root 6 Inner bird Root 7 Ogapi coat 8 Injin mugwort Stem, leaf 9 Peony Root 10 Earth Fruit 11 Chachocho all 12 Red ginseng Root

Experimental Example  2. Lactic Acid Bacteria of Medicinal Plant Extract Fermentation Broth ADL Activity and ALDH Active measurement

The fermentation broth obtained from Examples 1 to 6 was measured in ADH inhibitory activity and ALDH promoting activity is shown in Table 5. The experimental method was the same as that of Experimental example 1.

Table 5.

division ADH activity (%) ALDH activity (%) Lactobacillus bulgaricus KCTC 3188 -42.1 218.9 Streptococcus lactis KCTC 3191 -42.8 205.6 Bifidobacterium bifidum KCTC 3202 -37.7 224.3 Leuconostoc lactis KCTC 3528 -38.2 258.4 Lactobacillus delbruechii sub. sp. lactis KCTC3636 -48.2 285.1 Leuconostoc mesenteroides KCTC 3718 -43.5 274.5

As can be seen in Table 5, among the six lactic acid bacteria Lactobacillus delbruechii sub . sp . lactis (KCTC 3636) was found to have the best ADH inhibitory activity and ALDH activity.

Experimental Example  3. Medicinal plant extract and its Fermented products  About ADH , ALDH  compare

The medicinal plant extract (CMHE) obtained in step 1 of Example 5 and the medicinal plant extract fermentation broth (FCMHE) obtained through step 3 of Example 5 were measured and shown in Table 6. The measuring method was the same as that of Experimental example 1.

Table 6.

division ADH (%) ALDH (%) Medicinal Plant Extracts (CMHE) -20.22 173.20 Medicinal Plant Extract Fermentation (FCMHE) -62.63 280.17

In Table 6, ADH activity was -20.22% in medicinal plant extracts, whereas ADH inhibitory activity was -62.63% in medicinal plant extracts, and ALDH activity was 173.20% in medicinal plant extracts. Silver 280.17% ALDH promoting activity was found to increase about 1.6 times than the extract. These results are interpreted as the fermentation products produced through the fermentation process by microorganisms affect the hangover removal effect.

Experimental Example  4. Medicinal Plant Extracts from Rats Fermented product  Hangover Relief

48 male Sprague-Dawley (SD) male rats, weighing 80-100 g, were purchased from Damul Science (Korea) and pre-stocked with solid feed and water for 2 weeks, then weighed at 23-240 g. Experiments were performed after reseparation. In order to investigate the effects of extract fermentation on alcoholic hangover, blood alcohol concentration and acetaldehyde concentration were measured over time after alcohol intake. A control group administered with distilled water and an experimental group administered 50, 100, 200, 400 mg / kg each of the extract fermentation products according to Example 5, and a positive control group (PC, administered with a commercial hangover drink (condition) at 5 ml / kg) Six groups of positive control group were divided into 8 groups. Samples were administered orally once for each group, and after 30 minutes, 20% ethanol (15 mL / kg) was orally administered. After 1, 3, 5, and 7 hours, blood alcohol and acetaldehyde levels were measured. The temperature of the feeding room was maintained at 23 ± 1 ℃, humidity 50 ± 5%, and maintained a 12-hour contrast cycle. During the breeding period, water and diet were freely consumed.

Table 7.

Test group Test substance dose concentration EtOH (control) Distilled Water (5ml / kg) + 20% Ethanol (15ml / kg) HE-50 Medicinal Plant Fermentation (50mg / kg) + 20% Ethanol (15ml / kg) HE-100 Medicinal Plant Fermentation (100mg / kg) + 20% Ethanol (15ml / kg) HE-200 Medicinal Plant Fermentation (200mg / kg) + 20% Ethanol (15ml / kg) HE-400 Medicinal Plant Fermentation (400mg / kg) + 20% Ethanol (15ml / kg) PC (positive control) Condition (5 ml / kg) + 20% ethanol (15 ml / kg)

After 1, 3, 5, and 7 hours of alcohol administration, blood was collected from the carotid artery of the rat, left at room temperature for 30 minutes, and centrifuged at 1,500 rpm for 15 minutes to separate the supernatant. After mixing 290 μl of 0.3 M potassium phosphate buffer (pH 9) and 100 μl of 0.049 M NAD +, 100 μl of the sample blood sample was added thereto, and the absorbance was measured at 340 nm after 3 min at 20 ° C. (A1). In addition, 50 µl of ADH or ALDH was added to the above mixture, and the resultant was kept at 20 ° C for 5 minutes, and the absorbance was measured at 340 nm (A2). Based on this, the concentration of blood alcohol was quantified by the following formula.

Concentration = 0.7259 / 3.6 × ΔA

ΔA = sample (A2-A1)-blank (A2-A1)

The test results are expressed as mean ± standard error, and one-way analysis of variance (ANOVA) is performed. If significance is observed, Dunnett test is performed to find a test group that is significantly different from the control group. It was.

In order to confirm the hangover improvement effect of the medicinal plant extract fermentation is shown in Figure 1 by measuring the concentration of alcohol in the blood.

In the case of the control group, the blood alcohol concentration reached the highest level in 1 hour. In addition, after 1 hour, the blood alcohol concentration was significantly decreased by 35.8% in the extract fermented 400 mg / kg treatment group and the commercial hangover treatment group compared to the control group. At 3 hours after alcohol intake, the fermented broth extract 400 mg / kg showed 24.1% better efficacy than the commercial hangover treatment group.

In addition, blood acetaldehyde concentration is measured and shown in FIG. 2.

In the control group, the blood acetaldehyde concentration peaked after 5 hours and then gradually decreased, whereas the blood acetaldehyde concentration peaked after 3 hours in the group fed with fermented broth and commercial hangover beverage. And then decreased rapidly. The reduction of acetaldehyde in blood from 3 hours to 7 hours was 50 mg / kg of the extract fermentation solution -40.7%, -45.9% of the 100 mg / kg treatment group, -45.9% of the 200 mg / kg treatment group, -49.6%, 400 mg / kg. The treatment group -65.1% and the commercial hangover improvement drink -57.5% showed the greatest decrease in blood acetaldehyde in the extract fermentation solution 400 mg / kg treatment group.

Therefore, in this study, the blood alcohol concentration and acetaldehyde concentration were measured to confirm the hangover improvement effect of the extract fermentation broth. It showed better efficacy than the group and showed the most effective reduction of blood alcohol.

In addition, even after 3 hours of oral administration of ethanol, the decrease in the concentration of acetaldehyde in the blood was changed to 50 mg / kg of the treated fermentation solution -40.7%, -45.9% of the 100 mg / kg treatment group, and -45.9% of the 200 mg / kg treatment group. The decrease in fermentation extract 400 mg / kg treated group (-65.1%) was significantly higher than that of -49.6% and -57.5% of the commercial hangover beverages.

As a result of the experiment, it was confirmed that the extract fermentation broth of the present invention has an effect of reducing harmful physical and mental symptoms after alcohol consumption by reducing the concentration of acetaldehyde in blood, which is known as a major cause of hangover.

Production Example  One. Hidden  Ring manufacturing

The fermentation product prepared in Example 5 was highly concentrated to 40 ° Bx, and then powdered by using a spray drier, and then an excipient-free silver ring was prepared using a fluidized bed coater. Specifically, the highly concentrated fermented product was heated to a temperature of 60 ° C., suctioned at a temperature of 170 ° C., discharged to a temperature of 95 ° C., and spray-dried in an amount suitable for coating to obtain a powder. Next, the product temperature was maintained at a temperature of 75 ° C and then sprayed at a nozzle pressure of 2.0 ~ 3.0 bar was coated to a ring of a certain size to prepare a ring in the form of silver.

1 is a graph showing the blood alcohol concentration of experimental rats.

2 is a graph showing the acetaldehyde concentration in the blood of rats.

<Explanation of symbols in the drawings>

EtOH: control (distilled water + ethanol)

HE-50: extract fermentation 50 mg / kg + ethanol according to Example 5

HE-100: 100 mg / kg extract fermentation according to Example 5 + ethanol

HE-200: 200 mg / kg extract fermentation according to Example 5 + ethanol

HE-400: Extracted fermentation 400 mg / kg + ethanol according to Example 5

PC: Positive control group (commercial hangover drink (condition) + ethanol)

Claims (7)

Extract one or more medicinal plants selected from the group consisting of licorice, brown root, goji berry, macmundong, baekbong-ryeong, soybean, ogapi, injin mugwort, peony, earth pear, chajeoncho and red ginseng with water, C ₁ -C ₃ alcohol or mixture Preparing an extract (step 1); Adding a yeast mold-containing composition to the extract to obtain a saccharified extract through a saccharification process (step 2); And Fermentation of the saccharified extract with lactic acid bacteria to obtain a fermentation broth (step 3) comprising a medicinal plant extract fermentation prepared by the manufacturing method comprising as an active ingredient, hangover prevention or solution. delete The method of claim 1, The medicinal plant of step 1 is 1 to 10 parts by weight licorice, 10 to 30 parts by weight, 5 to 15 parts by weight of goji berry, 1 to 10 parts by weight of Macmundong, 1 to 10 parts by weight of Baekbyeongyeong, 5 to 15 parts by weight of genus vulgaris, Medicinal plants comprising 5 to 15 parts by weight, 5 to 15 parts by weight of Injin mugwort, 1 to 10 parts by weight, peony 1 to 10 parts by weight, 1 to 10 parts by weight of chajeoncho and 1 to 20 parts by weight of red ginseng Hangover prevention or remedy composition containing the extract fermentation as an active ingredient. The method according to claim 1 or 3, Extraction of step 1 is a composition for preventing or resolving hangover containing medicinal plant extract fermentation as an active ingredient, characterized in that extracted for 12 to 72 hours at a temperature of 80 to 100 ℃. The method according to claim 1 or 3, The saccharification process of step 2 is an active ingredient for medicinal plant extract fermentation, characterized in that for 1 to 10 parts by weight based on 100 parts by weight of koji fungus as a composition containing yeast fungi at 15 to 55 ℃ for 36 to 72 hours Hangover prevention or remedy composition containing. The method according to claim 1 or 3, The fermentation of the step 3 is medicinal, characterized in that carried out for 1 to 10 days at 15 to 55 ℃ using lactic acid bacteria of the genus Lactobacillus, Streptococcus, Pediococcus, Leukonostok or Bifidobacterium Hangover prevention or remedy composition containing plant extract fermentation as an active ingredient. Extract one or more medicinal plants selected from the group consisting of licorice, brown root, goji berry, macmundong, baekbong-ryeong, soybean, ogapi, injin mugwort, peony, earth pear, chajeoncho and red ginseng with water, C ₁ -C ₃ alcohol or mixture Preparing an extract (step 1); Adding a yeast mold-containing composition to the extract to obtain a saccharified extract through a saccharification process (step 2); Fermenting the saccharified extract with lactic acid bacteria to obtain a fermentation broth (step 3); Method of producing a hangover prevention or remedy comprising a medicinal plant extract fermentation containing as an active ingredient.

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