KR100958859B1 - Continuous flow-through system for Daphnia toxicity test - Google Patents

Abstract

The present invention relates to a flow-type water flea toxicity test apparatus, and more particularly, to expose a water flea (aquatic organisms) to a test solution (diluted water mixed with harmful substances) continuously supplied harmful substances while observing swimming and the like It is related to a flow type daphnia toxicity test device for testing environmental impact.

To this end, the present invention in the water flea toxicity test device for testing the effect of harmful substances on the environment,

A pumping unit for supplying dilution water and harmful substances, at least one mixing unit for preparing a test solution by mixing the dilution water and the hazardous substances supplied from the pumping unit, and a test solution continuously supplied from the mixing unit Consists of one or more exposed portions that expose

Provides a flow type daphnia toxicity test device, characterized in that by continuously supplying and replacing the test solution during the test period to suit the ecological characteristics of the daphnia to be tested.

Hazardous Substances, Toxicity, Environment, Dilution, Test, Evaluation, Eating, Water Flea

Description

Continuous flow-through system for Daphnia toxicity test}

The present invention relates to a flow-type water flea toxicity test apparatus, and more particularly, to expose a water flea (aquatic organisms) to a test solution (diluted water mixed with harmful substances) continuously supplied harmful substances while observing swimming and the like It is related to a flow type daphnia toxicity test device for testing environmental impact.

New damage caused by the increase of harmful substances due to industrialization (e.g. environmental hormone risk), ecological damage due to the increase of waste or pesticide components that are water pollution sources, serious severity of residual pesticides in food, heavy metal contamination in soil The human body and the environment are threatened by the back.

As such, monitoring the harmfulness of chemicals that adversely affect the human body and the environment has emerged as a very important and urgent task.

Currently, there are more than 80,000 chemicals used domestically and internationally. Thousands of new substances are used each year as raw materials for food, medicine, cosmetics, medical equipment, etc. Toxic effect of these substances on the human body and the environment It is very important to accurately identify the level of exposure and exposure.

As a field to secure the safety of such chemicals, there is an environmental hazard assessment, and there are known environmental toxicity-related test items required for this purpose, and the test items are almost directly or indirectly dangerous to humans and environmental organisms. To test and analyze the possibilities.

Through this environmental hazard assessment, all data related to exposure, degradation rate, concentration rate, and toxicity are tested and analyzed, and the results are aggregated to obtain a reasonable evaluation of a single compound. Can be.

To diagnose these environmental hazards, toxicity has been tested in several ways using daphnia, fish, microalgae, luminescent microorganisms, algae, activated sludge, and the like.

To protect the ecosystem from the chemicals or environmental pollutants and to promote sustainable development, we conduct toxicity tests of aquatic organisms (fish, daphnia, etc.) and terrestrial organisms on industrial chemicals, pesticides, veterinary medicines and pharmaceuticals. In addition, research is being conducted in parallel.

All test items are conducted in accordance with OECD test guidelines and test methods of each country such as the USA, Japan and Europe test guideline. The most important characteristic of the toxicity assessment for aquatic organisms is test substance (test substance solution). Has an indirect route of administration that dissolves in water and moves into the organism.

These exposure paths include a static test that does not replace the test solution during the test period, a semistatic test that exchanges all the test solution at regular intervals during the test period, and the test solution continuously exchanged during the test period. Because of the various flow-through tests, it is important to select the most appropriate exposure route for each test substance and test subject.

Among these, toxicity tests using aquatic organisms such as water fleas currently use static or semi-static equations, but when exposed to static or semi-static equations, the test is limited, which makes it difficult to obtain accurate test results.

Therefore, in view of the ecological characteristics of water fleas, it is desirable to use a test apparatus that is exposed to flow by increasing the accuracy of the test data.

However, the oil-and-water formula is manufactured manually by hand to maintain the concentration of the test material when the test material (harmful material) is not stable in the aqueous solution (water) state, there is a problem that the precision is lowered and continuous supply is difficult.

The present invention has been invented to solve the above problems, in order to measure the impact of harmful substances on the environment using aquatic organisms such as daphnia, the test solution during the test period in consideration of the ecological characteristics of the aquatic organisms The purpose is to provide a flowable daphnia toxicity tester.

In order to achieve the above object, the present invention provides a water flea toxicity test apparatus for testing the effects of harmful substances on the environment,

A water flea is provided to a pumping unit for supplying dilution water and harmful substances, at least one mixing unit for preparing a test solution by mixing the dilution water and the hazardous substances supplied from the pumping unit, and a test solution continuously supplied from the mixing unit. Consists of one or more exposed portions to expose,

Provides a flow-type toxicity test device characterized in that the test solution is continuously supplied and replaced during the test period so as to be compatible with the ecological characteristics of the daphnia.

In addition, the test solution supplied from the mixing unit is characterized in that it further comprises at least one branch comprising a lower chamber formed with two or more branch drain pipe for supplying to the exposed portion.

Preferably, the pumping unit, the tank containing the dilution water, a moving pump for moving the dilution water to the tank, a dilution water supply container for receiving the dilution water moved by the moving pump, the dilution water supply container is mounted A dilution water supply unit configured to include a level sensor for adjusting the dilution water level, a controller for controlling the operation of the moving pump, and at least one supply pump for moving the dilution water supplied to the dilution water supply container;

A hazardous substance input unit including a hazardous substance supply container accommodating a hazardous substance, one or more micropumps for moving the hazardous substances from the hazardous substance supply container, and a micropump controller for controlling the operation of the micropumps; Characterized in that comprises a,

The mixing unit, the upper chamber for accommodating dilution water and harmful substances supplied from the pumping unit, a stirring blade installed inside the upper chamber to mix dilution water and harmful substances, and a motor for supplying a driving force to the stirring blades And a coupling for transmitting the driving force of the motor to the stirring vane, and a discharge hose for discharging the test solution to the upper chamber to the outside.

The branch part may be disposed in an upper part of the lower chamber to receive a test solution supplied from a mixing part, a locking member fastened through an O-ring at a lower end of the intermediate chamber, and penetrating the intermediate part through the locking member. Characterized in that it further comprises a siphon that is inserted into the interior of the chamber.

The exposure unit may include a test beaker including an input tube for receiving a test solution to be supplied and a drain pipe for discharging the test solution, and a test container in which the water flea is placed. .

The flowable water flea toxicity test apparatus according to the present invention can be carried out by applying the flow type exposure method suitable for the ecological characteristics of aquatic organisms, and thus it is possible to collect accurate test data.

Therefore, it can be expected that the environmental hazard assessment for the hazardous substance is made more accurately.

The terminology used herein is for the purpose of describing particular example embodiments only and is not intended to be limiting of the invention, and the singular forms “a”, “an” and “the” include plural forms unless the context clearly indicates otherwise.

There may be a plurality of embodiments of the present invention, and overlapping descriptions of the same parts as in the prior art may be omitted.

Hereinafter, with reference to the accompanying drawings will be described in detail a preferred embodiment of the present invention.

The flow type daphnia toxicity test apparatus, which is preferably implemented according to the present invention, applies a flow type exposure method suitable for the ecological characteristics of water fleas (aquatic organisms) in order to increase the accuracy of collected test data in order to grasp the effect of certain harmful substances on the environment. Configured to do so.

Accordingly, the present invention can be used to check the swimming status of water fleas according to the harmful concentrations of specific chemicals, and the harmful concentrations for deriving a half-prime test subject.

FIG. 1 is a schematic flowchart of a flow-type toxicity test apparatus according to the present invention, and FIG. 2 is an overall configuration diagram illustrating an embodiment of the flow-type water flea toxicity test apparatus according to the present invention.

As shown in FIG. 1, an embodiment of the present invention includes a pumping unit for supplying dilution water and a test substance (harmful substance), a mixing unit for mixing the supplied dilution water and hazardous substances evenly, and a mixed test It consists of a branch for distributing a solution (a solution in which dilution water and harmful substances are mixed) and an exposed part for exposing the water flea to the distributed test solution.

Figure 3 is a configuration diagram showing in detail a part of the flow type daphnia toxicity test apparatus according to the present invention.

The pumping unit is composed of a dilution water supply unit for supplying dilution water to the mixing unit and a hazardous substance input unit for injecting harmful substances. 2 and 3, the dilution water supply unit includes a tank 10 containing a large amount of dilution water, a moving pump 11 for moving the dilution water contained in the tank 10, and the movement. A dilution water supply container 13 receiving dilution water through a pump 11, a level sensor 14 mounted on an inner upper end of the dilution water supply container 13 to adjust a water level, and the dilution water supply container And a feed pump 15 for moving the dilution water contained in 13 to the mixing unit.

The dilution water accommodated in the tank 10 is synthetic water prepared to normally culture the water flea under test with M4 and M7 medium prepared according to the Organization for Economic Cooperation and Development (OECD) standards.

The moving pump 11 sucks dilution water into the tank 10 through a hose and moves to the dilution water supply container 13. When the dilution water fills up to a predetermined level in the dilution water supply container 13, the water level is filled. The sensor 14 detects this and sends a detection signal to the controller 12 that controls the operation of the moving pump 11, and the controller 12 temporarily stops the operation of the moving pump 11 to stop supplying the dilution water. Let's do it. When the dilution water is drained and the water level of the dilution water supply container 13 is lowered, the water level sensor 14 detects this and operates the mobile pump 11 to supply the dilution water again.

Here, the supply pump 15 is turned on (ON) / off (OFF) by a switch installed separately, the operation is controlled, it is composed of a plurality in one dilution water supply container 13 to move the dilution water respectively. Can be.

Next, the hazardous material input unit and the hazardous material supply container 16 for accommodating a specific hazardous material, a micro pump 17 for moving the hazardous material contained in the hazardous material supply container 16, and the micro pump 17 It is configured to include a micropump controller 18 to control the operation of.

The micro pump 17 is turned on by a switch to pump a hazardous substance in the hazardous substance supply container 16 and move the pump, and the micro pump controller 18 pumps a very small amount of hazardous substance. You can move it.

On the other hand, the mixing unit supplied with the harmful substances by the micro-pump 17, the upper chamber 20 for receiving the supplied dilution water and harmful substances, and the inside of the upper chamber 20 is diluted with dilution water and harmful Agitation blade 23 for rotating the movement so that the material is unevenly mixed evenly, a motor 21 is provided at the lower end of the upper chamber 20 to provide a rotational force to the stirring blade 23, the motor 21 It is configured to include a coupling 22 is connected to the shaft of the) to transfer the rotational force to the stirring blade (23).

The coupling 22 is preferably composed of a magnetic coupling so as not to interfere with the upper chamber 20. For this purpose, the upper chamber 20 is made of a glass material which is not affected by magnetic force.

Dilution water and harmful substances supplied to the upper chamber 20 are mixed evenly by the stirring blade 23 as the motor 21 is operated by the switch (ON). In addition, a discharge hose 24 is installed in the upper chamber 20 to discharge the mixed test solution, and the discharge hose 24 is introduced into the intermediate chamber 30 of the branch part to continuously supply the test solution. do.

The branch portion includes an intermediate chamber 30 to which a test solution is supplied, and a siphon 31 installed inside the intermediate chamber 30. The siphon 31 is composed of an inner tube 31a inclined at an upper end and an outer tube 31b formed in a circumference larger than the inner tube 31a and closed at an upper end thereof. 31b) is inserted into and attached to one side.

When the test solution is supplied to the intermediate chamber 30 and rises above the level of the upper end of the inner tube 31a, the test is caused by the difference between the inner pressure of the outer tube 31b and the inner pressure of the intermediate chamber 30. The solution flows outward through the inner tube 31a. At this time, the test solution flows until the internal pressure of the outer tube 31b and the internal pressure of the intermediate chamber 30 are balanced, and is moved to the lower chamber 35 positioned at the lower end of the intermediate chamber 30.

A lower end of the intermediate chamber 30 is provided with a threaded locking member 32 for fixing the inner tube 31a. The locking member 32 penetrated by the inner tube 31a is inserted and installed through the O-ring 33 at the lower end of the intermediate chamber 30. When the locking member 32 is rotated to one side, the O-ring is installed. (33) is pressed to be fixed while being in close contact with the surface of the inner tube (31a). Thus, the locking member 32 and the O-ring 33 can be fixed by adjusting the height of the siphon 31, and the test solution filled in the intermediate chamber 30 according to the height of the siphon 31. You can adjust the amount and one-time volume.

In addition, a bypass tube 34 capable of arbitrarily discharging the test solution is provided on the outside of the intermediate chamber 30 to prevent the siphon 31 from being drained and overflowing when the test solution is malfunctioned.

In addition, the bypass pipe 34 is formed at an upper position of the siphon 31 to discharge the test solution only when the siphon 31 malfunctions.

On the other hand, the lower chamber 35 is a beaker-shaped container with an open upper end portion provided with a plurality of branch drain pipes 36 for discharging the test solution at the lower end, and overflows the test solution moved from the intermediate chamber 30. In order to prevent the bypass pipe 37 is provided. Then, the rubber hose 38 is connected to the branch drain pipe 36 to supply the test solution to the exposed part.

The exposed part includes a test beaker 40 having an input pipe 40a through which a test solution is supplied and a drain pipe 40b through which the test solution is discharged, and a test object is placed inside the test beaker 40. The test vessel 41 to be built in.

The lower end of the test container 41 is configured in a mesh shape so that the entrance and exit of the test solution can be made freely, and the side portion thereof is non-mesh type so that the water flea under test is not affected by the flow and pressure of the test solution. Is formed.

Alternatively, the test container 41 may be configured by incorporating an inner container provided in a mesh shape into an outer container having a lower end portion provided in a non-mesh type.

In one embodiment according to the present invention, the mixing part, the branch part, and the exposed part as described above may be composed of a plurality of tests at the same time, and as shown in FIG. 2, one dilution water supply container 13 And dilution water and harmful substances to the plurality of upper chambers 20 in the hazardous substance supply container 16.

In addition, the present invention comprises a plurality of upper chambers by configuring a combined pump that can replace the micro pump 17 and the supply pump 15, or by configuring a single pump that can replace a plurality of supply pump 15 ( It is also possible to configure dilution water in 20).

Hereinafter, an operation state of an embodiment according to the present invention configured as described above is as follows.

When a large amount of dilution water contained in the tank 10 is moved to the dilution water supply container 13 by the transfer pump 11, each supply pump 15 is operated to move the dilution water to the upper chamber 20. . At this time, the micro pump 17 also operates to supply the hazardous materials to the upper chamber 20.

Noxious substances and dilution water moved to the upper chamber 20 are evenly mixed by the stirring blade 23 is provided as a test solution of a certain concentration and is continuously supplied to the intermediate chamber (30).

When the test solution contained in the intermediate chamber 30 becomes higher than the siphon 31, the test solution is drained and moved to the lower chamber 35 according to the conventional siphon principle as described above.

At this time, the amount of the test solution discharged once is adjusted by changing the position of the siphon 31 (position in the intermediate chamber).

The test solution moved to the lower chamber 35 is branched along the branch drain pipe 36 and continuously introduced into the test beaker 40 of the exposed part, and the test solution introduced into the test beaker 40 is the drain pipe 40b. Through the discharge is continuously.

Accordingly, the water fleas placed in the test container 41 are exposed to the test solution to be replaced in succession and tested on ecological conditions similar to the real environment.

While the invention has been shown and described with respect to certain preferred embodiments, the invention is not limited to these embodiments, and those of ordinary skill in the art claim the invention as claimed in the appended claims. It includes all embodiments of the various forms that can be carried out without departing from the spirit.

1 is a schematic flowchart of a flow-type daphnia toxicity test apparatus according to the present invention,

Figure 2 is an overall configuration showing an embodiment of the flow type water flea toxicity test apparatus according to the present invention,

Figure 3 is a schematic view showing in detail a part of the flow type water flea toxicity test apparatus according to the present invention,

Figure 4 is an exemplary view showing a part of the flow type daphnia toxicity test apparatus according to the present invention.

<Description of the symbols for the main parts of the drawings>

10 tank 11: moving pump

13: dilution water supply container 14: water level sensor

15: supply pump 16: hazardous material supply container

17: micro pump 18: micro pump controller

20: upper chamber 21: motor

22: coupling 23: stirring blade

30: intermediate chamber 31: siphon

32: locking member 33: O-ring

35: lower chamber 36: branch drain pipe

40: test beaker 41: test container

Claims (10)

delete delete delete delete A pumping unit for supplying dilution water and harmful substances, at least one mixing unit for preparing a test solution by mixing the dilution water and the hazardous substances supplied from the pumping unit, and a test solution continuously supplied from the mixing unit In the water type flea toxicity test apparatus comprising at least one exposed portion for exposing and at least one branch for distributing and supplying the test solution supplied from the mixing portion to the exposed portion, The branch portion, the lower chamber 35 is formed with one or more branch drain pipe (36); An intermediate chamber 30 disposed above the lower chamber 35 to receive a test solution supplied from the mixing unit; A locking member 32 fastened via an O-ring 33 at a lower end of the intermediate chamber 30; It is inserted into the inside of the intermediate chamber 30 through the locking member 32, the outer tube is formed in a circumference larger than the inner tube 31a and the upper end is obliquely inclined at the upper end is closed A siphon 31 composed of a tube 31b; By configuring Water-flow type flea toxicity test apparatus, characterized in that the test solution can be continuously supplied and replaced during the test period to suit the ecological characteristics of the water flea being tested. A pumping unit for supplying dilution water and harmful substances, at least one mixing unit for preparing a test solution by mixing the dilution water and the hazardous substances supplied from the pumping unit, and a test solution continuously supplied from the mixing unit In the water type flea toxicity test apparatus comprising at least one exposed portion for exposing and at least one branch for distributing and supplying the test solution supplied from the mixing portion to the exposed portion, The exposed part includes a test beaker 40 having an input tube for receiving a test solution and a drain pipe for discharging the test solution, and a test container 41 embedded in the test beaker 40 and having a test object therein. Constituent, the test container (41) is a non-mesh-type water flea toxicity test device, characterized in that the inner container formed in the mesh shape inside the outer container of the lower end opening is configured. The method according to claim 5, Flow-type water flea toxicity test device, characterized in that the lower chamber 35 of the branch portion is further formed with a bypass tube (37) for discharging the test solution to the outside. delete The method according to claim 5, Flow-type water flea toxicity test apparatus, characterized in that the intermediate chamber 30 of the branch portion 30 is further formed with a bypass tube 34 for discharging the test solution to the outside during the malfunction of the siphon. delete

Images