KR101927307B1 - Apparatus for testing immobilisation of daphnia in closed system and method for acute toxicity test using the apparatus - Google Patents

Abstract

The apparatus for evaluating a water droplet swim according to the present invention comprises a water tank for containing a test solution containing a water flea, a rotating part at least a part of which is disposed in the test solution so as to generate water for stimulating the water flea, And a controller for controlling the camera to capture the image of the water flea for a predetermined period of time after the rotation of the propeller is completed and analyzing the image of the water flea taken to acquire information about the swimming ability of the water flea.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to an apparatus for evaluating a water droplet in a closed system, and a method for evaluating acute toxicity using the apparatus.

The present invention relates to an apparatus for evaluating a water droplet for evaluation of toxicity and an acute toxicity evaluation method using the same.

There are various types of damages caused by the increase of harmful substances due to industrialization. Environmental hormones that disturb the endocrine system, wastewater and wastes threatening the aquatic ecosystem, pesticides remaining in food, heavy metals pollution in the soil, Health is threatened.

It is very important and urgent to monitor the toxic effects of chemicals and evaluate their risks.

Currently, more than 80,000 chemical substances are used commercially at home and abroad, and thousands of new substances are developed each year, which are used as raw materials for foods, medicines, cosmetics, medical equipment, etc. How much do they affect the human body and the environment It is very important to accurately identify toxic effects and exposure levels.

As an area for securing the safety of chemical substances in the environment, 'Environmental Risk Assessment' is being implemented. All the data such as exposure dose, decomposition rate, enrichment rate and toxicity are tested and analyzed, It is the risk that a chemical can cause in the environment.

In order to evaluate the toxicity required, living organisms such as daphnia, fishes, microalgae, luminescent microorganisms, and algae are being utilized.

In order to protect the ecosystem from the above chemical substances or environmental pollutants and to promote sustainable development, toxicological tests of aquatic organisms (fish, daphnia, etc.) and terrestrial organisms are carried out on industrial chemicals, pesticides, veterinary drugs and medicines In addition, studies related to this are being carried out.

All tests are carried out in accordance with the OECD Test Guideline and test methods in the United States, Japan and Europe. The most important characteristic of the toxicity test for aquatic organisms is the test substance (test substance solution) Is dissolved in water and exposed to organisms.

In the OECD Guidelines for the Testing of Chemicals (Test No. 202), aquatic organisms such as daphnia were exposed to the test substance in the test vessel for 48 hours and then evaluated for loss of swimming ability. Observe whether swimming within a given time (for example, 15 seconds).

Therefore, several devices (stimulating devices and swimming evaluation devices) need to be designed to implement the automation of the TG 202 test method. In addition, when such a device is applied to a volatile substance, a closed system condition must be realized. In order to analyze the concentration of the test substance and the physicochemical characteristics of the cultured water, There is a need to do.

It is an object of the present invention to provide an apparatus for evaluating a dive flap capable of performing the OECD TG 202 test method by devising a stimulation apparatus and a swimming evaluation apparatus.

Another object of the present invention is to provide a daphnia slide evaluation apparatus capable of more easily collecting a test solution in a closed system water tank.

The apparatus for evaluating a water droplet swim according to the present invention comprises a water tank for containing a test solution containing a water flea, a rotating part at least a part of which is disposed in the test solution so as to generate water for stimulating the water flea, And a controller for controlling the camera to capture an image of the daphnia for a predetermined period of time after the operation of the rotation unit is completed, and analyzing the image of the daphnia photographed to acquire information on the swimming ability of the daphnia.

The apparatus may further include at least one drain pipe disposed on one side of the test solution and on the other side of the water tank.

The apparatus for evaluating a water droplet swimmer according to an embodiment may further include a water level adjusting unit disposed inside the water tank for fixing the test solution contained in the water tank and fixing the discharge pipe.

In the embodiment, the rotating portion may include a propeller, a pressure portion disposed on the outside of the water tank so as to be able to receive an external pressure, and a pressure portion connected to the pressure portion and the other portion connected to the propeller, .

The apparatus for evaluating a dappling fluid according to an embodiment of the present invention may further include a cover disposed to cover the rotation shaft and the discharge pipe so as to cover the water tank.

In an embodiment, the level adjusting portion may be fixed to the rotation shaft.

In the apparatus for evaluating a water droplet swimming according to an embodiment, a beaker in which a test solution discharged to the outside through a discharge pipe is received may be displayed based on information on the swimming ability of the water flea.

In an embodiment, the control unit controls the external pressurizing device to apply a predetermined pressure to the pressure unit after the camera captures an image of the daphnia, so that a part of the test solution is discharged to the outside through the discharge pipe .

A method for evaluating a daphnia toxicity using a daphnia swimming evaluation apparatus having a water tank for containing a test solution containing a daphnia according to the present invention comprises the steps of generating water to stimulate the daphnia in the test solution, Capturing an image of the water flea through a camera for a preset time and analyzing the image of the water flea to acquire information on the swimming ability of the water flea.

According to an embodiment, the method may further include displaying a beaker in which a test solution discharged to the outside through a discharge pipe is received, based on information about the swimming ability of the daphnia.

The present invention can be applied to environmental or ecotoxicity test methods such as evaluation of ecotoxicity, search for toxic substances and reduction measures by evaluating the toxicity of a test solution by observing swimming in aquatic invertebrates such as daphnia .

The present invention has an advantage that it can be effectively utilized for environmental risk assessment for ensuring the safety of chemical substances as environmental problems due to increase in chemical substances due to industrialization become serious.

FIG. 1 illustrates a device for evaluating a water droplet swimming according to an embodiment of the present invention.
FIG. 2 is a conceptual diagram for explaining the operation of the apparatus for evaluating a dive flap according to an embodiment of the present invention.
FIG. 3 is a front view of a state in which some components of the apparatus for evaluating a water droplet are combined according to an embodiment of the present invention.
FIG. 4 is a bottom view of a state in which some components of a daphnid evaluation apparatus according to an embodiment of the present invention are combined.
FIG. 5 is a plan view showing a state in which some components of the apparatus for evaluating a daphnia are combined according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings, wherein like or similar components are denoted by the same reference numerals, and redundant explanations thereof will be omitted. The suffix "module" and " part "for the components used in the following description are given or mixed in consideration of ease of specification, and do not have their own meaning or role. In the following description of the embodiments of the present invention, a detailed description of related arts will be omitted when it is determined that the gist of the embodiments disclosed herein may be blurred. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are intended to provide further explanation of the invention as claimed. , ≪ / RTI > equivalents, and alternatives.

Terms including ordinals, such as first, second, etc., may be used to describe various elements, but the elements are not limited to these terms. The terms are used only for the purpose of distinguishing one component from another.

It is to be understood that when an element is referred to as being "connected" or "connected" to another element, it may be directly connected or connected to the other element, . On the other hand, when an element is referred to as being "directly connected" or "directly connected" to another element, it should be understood that there are no other elements in between.

The singular expressions include plural expressions unless the context clearly dictates otherwise.

In the present application, the terms "comprises", "having", and the like are used to specify that a feature, a number, a step, an operation, an element, a component, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

Hereinafter, an apparatus for evaluating a water droplet swim according to the present invention will be described with reference to the drawings.

FIG. 1 illustrates a device for evaluating a dive flap according to an embodiment of the present invention, and FIG. 2 is a conceptual view for explaining an operation of the dive flap evaluation device according to an embodiment of the present invention. FIGS. 3 to 5 are a front view, a bottom view, and a plan view, respectively, of a state in which some components of the apparatus for evaluating a water droplet are assembled according to an embodiment of the present invention.

The apparatus for evaluating a dapple swimming according to the present invention may include a water tank 100, a rotation unit 200, a camera 500, and a control unit.

The water tank 100 may be configured to receive the test solution. For example, the water tank 100 may be a beaker in which one side is opened so as to allow the inflow of the test solution. At this time, a scale may be displayed on the side surface of the water tub 100 so as to easily grasp the amount of the test solution. Further, the water tank 100 may be made of a light-transmitting material.

Here, the test solution may mean a solution to be subjected to toxicity evaluation. In addition, the water fleas to be subjected to the swimming evaluation may be contained in the test solution.

The rotary unit 200 may include a pressure unit 210, a rotary shaft 220, and a propeller 230.

The propeller 230 may be disposed in the test solution to produce a water droplet stimulating the daphnia.

The pressure portion 210 may be disposed outside the water tub 100 so as to be able to receive external pressure. The external pressure may be applied by an external pressurizing device or by an operator. The external pressurizing device can be controlled by the control unit. In this case, the pressure of the pressure portion 210 can be controlled by the control portion.

A motor may be provided inside the pressure unit 210. The motor can provide a driving force for rotation of the propeller 230. The motor may be controlled by a control unit. In this case, the control unit controls the rotation of the motor, the rotation speed, and the like.

The rotary shaft 220 connects the pressure unit 210 and the propeller 230. Specifically, the pressure portion 210 is connected to one side of the rotary shaft 220, and the propeller 230 is connected to the other side. That is, the rotation of the motor can be transmitted by the rotation of the propeller 230 by the rotation axis 220.

The apparatus for evaluating a water droplet swimmer according to an embodiment of the present invention may further include a water level controller 320 and a discharge pipe 400.

The water level control part 320 is disposed inside the water tub 100 to limit the test solution contained in the water bath 100. [ More specifically, the diameter of the water level regulating part 320 may be substantially the same as the inner diameter of the water tub 100. Here, the fact that the two numerical values are substantially coincident may include that the numerical values have a difference within a predetermined range, including numerically identical numerical values.

The water level control part 320 may be formed of a rubber material for packing. According to this structure, the test solution located below the water level control part 320 does not leak to the upper part of the water level control part 320.

The water level control unit 320 may be fixed to the rotation shaft 220 of the rotation unit 200. Accordingly, when the rotary shaft 220 is moved, the water level adjusting part 320 can be moved together in the water tub 100. [

One side of the discharge pipe 400 may be disposed inside the test solution and the other side may be disposed outside the water tank 100. The drain pipe 400 may be fixed to the water level controller 320 so that the drain pipe 400 is moved together when the water level controller 320 moves within the water tank 100.

The discharge pipe 400 may be a glass tube.

On the other hand, a straight discharge pipe 400 is shown in the drawing, but the present invention is not limited thereto. For example, the discharge tube 400 may have a bent portion bent at least once. The test solution discharged to the outside can be collected more easily than the case of the discharge pipe 400 having at least one bent portion.

Meanwhile, one exhaust pipe 400 is shown in the drawing, but the present invention is not limited thereto. For example, a plurality of discharge pipes 400 may be connected to one water tank 100. In this case, the test solution discharged from each discharge pipe 400 may be collected in different beakers.

The apparatus for evaluating a dapple swimming according to an embodiment of the present invention may further include a cover 310. The lid 310 is configured to cover the water tub 100. Meanwhile, the lid 310 may be referred to as a cap 300 together with the water level controller 320.

The lid 310 may be configured to allow the rotation shaft 220 and the discharge pipe 400 to pass therethrough. More specifically, the cover 310 may be formed with first and second through-holes. That is, the rotating shaft 220 and the discharge pipe 400 can penetrate through the first and second through holes, respectively. At this time, the rotary shaft 220 and the discharge tube 400 may not be fixed to the cover 310 so as to be movable in the first and second through holes.

Hereinafter, with reference to FIG. 2, the operation of the apparatus for evaluating a dive flap according to an embodiment of the present invention will be described.

2 (a) and 2 (b) show a state in which external pressure is not applied to the pressure portion 210 and an applied state, respectively.

As described above, the test solution is contained in the water tank 100, and the daphnia is swimming in the test solution. At this time, the level controller 320 defines a space in which the test solution is received. At this time, since the propeller 230 is used to generate a light water that stimulates the daphnia, the propeller 230 can be positioned as close as possible to the water level controller 320 so as not to disturb the swimming of the daphnia.

Referring to FIG. 2, when external pressure is applied to the pressure portion 210, the rotary portion 200 is entirely moved in a direction in which the external pressure is applied. Specifically, as the rotary part 200 moves downward, the water level adjusting part 320 fixed to the rotary part 200 (more specifically, fixed to the rotary shaft 220) also moves downward in the water tub 100 Move. As the lower space of the water level regulating part 320 is reduced, the pressure is increased, and thus the test solution is discharged to the outside through the discharge pipe 400. At this time, the discharge pipe 400 may be connected to the external beaker so that the discharged test solution is accommodated.

Up to now, the structure and operation of the apparatus for evaluating the swimming of a water droplet according to the embodiment of the present invention have been described in detail. Hereinafter, a control method of the control unit of the dive bleeding evaluation apparatus according to an embodiment of the present invention will be described.

According to one embodiment of the present invention, the camera 500 is configured to capture an image of a daphnia. For example, as shown in FIG. 2, the camera 500 may be installed outside the water tub 100, or may be installed inside the water tub 100. In addition, the camera 500 may be configured to track the swimming fleas of the water based on the image of the previously stored water fleas.

The control unit may control the camera 500 to photograph an image of the daphnia for a predetermined time after the rotation of the propeller 230 is completed. Specifically, the controller may photograph the daphniae of the daphniae through the camera 500 for a predetermined time immediately after the propeller 230 controls the motor to rotate for a preset time. For example, the camera 500 may capture an image of daphnia for about 15 seconds.

The control unit may analyze the image of the daphnia acquired through the camera 500 to acquire information on the swimming ability of the daphnia.

For example, the control unit may acquire the first detailed information when it is determined that the diving fleet is capable of swimming, and may acquire the second detailed information when it determines that the dive fleeting ability is not available. Alternatively, the information on the swimming ability may be information that further refines the swimming ability of the daphnia.

The control unit may analyze the image of the daphniae by a predetermined method. For example, the predetermined method may be an algorithm for measuring the distance traveled by the daphnia.

Further, the control unit may display a beaker that receives the test solution discharged to the outside based on the acquired information on the swimming ability of the daphnia.

For example, the control unit may control the labeling device that labels the beaker. At this time, if the first detailed information is acquired, the control unit attaches a label including the first detailed information to the beaker, and if the second detailed information is acquired, the label including the second detailed information is attached to the beaker The labeling device can be controlled.

Alternatively, the control unit can control the position of the beakers so that the other kind of beaker is disposed on one side of the end of the discharge pipe 400, based on the information about the swimming ability of the acquired daphnia. For example, a first type of beaker is disposed at the end of the discharge tube 400 so that the test solution is received in the first type of beaker when the control unit obtains the first detailed information. When the control unit obtains the second detailed information, the second kind of beaker is disposed at the end of the discharge pipe 400 so that the test solution is received in the second kind of beaker.

Meanwhile, the control unit may control the external pressurizing device to apply a predetermined pressure to the pressure unit 210 after the camera 500 captures an image of the water flea. Accordingly, at least a part of the test solution can be automatically discharged to the outside through the discharge pipe 400. The test solution discharged to the outside as described above can be accommodated in the prepared beaker.

Furthermore, the control unit can sequentially control the motor, the camera 500, and the external pressurizing device. According to this, after the motor is driven for a preset time, after the image of the daphnia is taken through the camera 500, external pressure is applied to the pressure portion 210 through the external pressurizing device to discharge the test solution to the outside .

The method for evaluating the toxicity of a water droplet according to the present invention includes the steps of generating water to stimulate the daphnia through a propeller 230 disposed in the test solution, 500), and acquiring information on the swimming ability of the daphnia by analyzing the image of the dapple. The method may further include displaying a beaker in which the test solution discharged to the outside through the discharge pipe 400 is received, based on the information about the swimming ability of the daphnia.

The present invention described above can be embodied as computer-readable codes on a medium on which a program is recorded. The computer readable medium includes all kinds of recording devices in which data that can be read by a computer system is stored. Examples of the computer readable medium include a hard disk drive (HDD), a solid state disk (SSD), a silicon disk drive (SDD), a ROM, a RAM, a CD-ROM, a magnetic tape, a floppy disk, , And may also be implemented in the form of a carrier wave (e.g., transmission over the Internet). Also, the computer may include a control unit of the terminal. Accordingly, the above description should not be construed in a limiting sense in all respects and should be considered illustrative. The scope of the present invention should be determined by rational interpretation of the appended claims, and all changes within the scope of equivalents of the present invention are included in the scope of the present invention.

Claims (10)

A water tank for containing a test solution containing a daphnia;
A rotating part in which at least a part is disposed in the test solution so as to generate water to stimulate the daphnia;
A camera for capturing an image of the daphnia;
A controller for controlling the camera to capture an image of the daphnia for a predetermined period of time after the operation of the rotation unit is completed, and analyzing the image of the dapple to acquire information on the swimming ability of the dapple;
A water level adjuster movably disposed inside the water tank to define a test solution contained in the water tank;
And at least one drain pipe disposed outside the water tank, one side of which is disposed inside the test solution so that at least a part of the test solution is discharged to the outside when the level control unit is moved. delete The method according to claim 1,
And the discharge pipe is fixed to the water level adjusting unit. The method according to claim 1,
Wherein the rotating portion includes a propeller,
A pressure portion disposed outside the water tank so as to be able to receive an external pressure and having a motor therein,
Further comprising a rotating shaft connected to the pressure unit at one side and to the propeller at the other side. 5. The method of claim 4,
Further comprising a lid which is configured to penetrate the rotary shaft and the discharge pipe and to cover the water tank. 5. The method of claim 4,
And the water level adjusting unit is fixed to the rotating shaft. The method according to claim 1,
And a beaker in which the test solution discharged to the outside through the discharge pipe is received is displayed based on information about the swimming ability of the daphnia. 5. The method of claim 4,
Wherein the control unit controls the external pressurizing device to apply a predetermined pressure to the pressure unit after the camera captures an image of the daphnia and a part of the test solution is discharged to the outside through the discharge pipe, . A water level control part movably arranged inside the water tank so as to confine the test solution contained in the water tank, and a water level control part having one side disposed inside the test solution and the other side disposed at the outside of the water tank A method for evaluating the toxicity of daphnia using a daphnia swimming evaluation apparatus comprising one or more discharge pipes,
Generating water to stimulate the water fleas in the water tank;
Capturing an image of the water flea through a camera for a predetermined period of time after the step of generating the water is completed;
Analyzing the images of the water fleas to obtain information on the swimming ability of the water fleas;
And controlling the external pressurizing device such that the level control part is moved so that at least a part of the test solution is discharged to the outside through the discharge pipe. 10. The method of claim 9,
Further comprising the step of displaying a beaker in which the test solution discharged to the outside through the discharge pipe is received, based on the information on the swimming ability of the daphnia.

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