KR100958122B1 - SNP gene set for identifying individual and bloodline of Hanwoo - Google Patents

SNP gene set for identifying individual and bloodline of Hanwoo Download PDF

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KR100958122B1
KR100958122B1 KR1020070128316A KR20070128316A KR100958122B1 KR 100958122 B1 KR100958122 B1 KR 100958122B1 KR 1020070128316 A KR1020070128316 A KR 1020070128316A KR 20070128316 A KR20070128316 A KR 20070128316A KR 100958122 B1 KR100958122 B1 KR 100958122B1
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Abstract

본 발명은 한우 개체인식 및/또는 혈통확인을 위한 SNP(single nucleotide polymorphism) 유전자 세트를 제공한다. 본 발명에서는 한우와 수입육을 판별할 수 있는 SNP 바이오마커 개발을 위해 NCBI 소(Bovine) SNP DB와 AFNS 소(Bovine) SNP DB에서 3,072개의 SNP를 선발하여 한우 570 두에서 지노타이핑(genotyping)을 실시하였다. 실험결과 3,072개 SNP 가운데 2,402개가 성공적으로 지노타이핑이 되었으며 MAF(minor allele frequency)가 0.3 이상인 SNP 총 384개를 선발하여 이를 한우 개체인식 및 혈통확인 유전자 세트로 이용하였다. 총 384개 유전자 세트의 실증성 검증을 위해 한우 570두에서 한우 개체인식 능력을 검사하였다. 분석결과 정확도 100%로 개체인식이 가능하였다. 또한 동일 유전자 세트를 이용하여 한우 종모우 57 두와 후대소 139 두의 혈통 추적 검사에서 100%의 정확도로 혈통확인이 가능하였다. 따라서, 본 발명의 대규모 SNP 지노타이핑으로 발굴된 384개의 SNP 유전자 세트는 한우 개체인식, 혈통확인, 원산지 판별에 적합하며, 이는 한우생산이력체계의 유전자 검사방법으로 적용가능하다. The present invention provides a single nucleotide polymorphism (SNP) gene set for Hanwoo individual identification and / or lineage identification. In the present invention, 3,072 SNPs were selected from NCBI Bovine SNP DB and AFNS Bovine SNP DB to develop SNP biomarkers for discriminating Korean cattle and imported meat, and genotyping was performed in 570 Korean cattle. It was. As a result, 2,402 of the 3,072 SNPs were successfully genotyped, and a total of 384 SNPs with a minor allele frequency (MAF) of 0.3 or more were selected and used as a set of gene recognition for Hanwoo. Hanwoo individual recognition ability was tested in 570 Korean cattle for the validation of a total of 384 gene sets. As a result of analysis, individual recognition was possible with 100% accuracy. Using the same gene set, it was possible to confirm the lineage with 100% accuracy in the follow-up test of 57 cows and 139 cows. Therefore, the 384 SNP gene sets discovered by the large-scale SNP genotyping of the present invention are suitable for Korean cattle individual recognition, pedigree identification, and origin determination, which can be applied as a genetic test method of the Hanwoo production history system.

한우, SNP, 개체인식, 혈통확인 Korean Cattle, SNP, Individual Identification, Pedigree Identification

Description

한우 개체인식 및 혈통확인을 위한 ＳＮＰ 유전자 세트 {SNP gene set for identifying individual and bloodline of Hanwoo}SNP gene set for identifying individual and bloodline of Hanwoo}

본 발명은 한우생산이력체계의 유전자 검사방법으로서, 한우 개체인식과 혈통확인을 위한 SNP 유전자 세트에 관한 것이다. The present invention relates to a genetic test method of the Hanwoo production history system, and a SNP gene set for Hanwoo individual identification and lineage identification.

건강에 대한 관심이 늘어나면서 식육에 대한 소비자들의 요구도 단순히 양적인 측면에서 안정성과 질 위주로 변화하고 있으며, 특히 생산지(브랜드) 그리고 생산에서 소비에 이르는 유통과정상의 신뢰성을 중요하게 생각하고 있다. 이런 변화에 따라 유럽연합에서는 유통되는 소고기에 대한 생산이력제를 실시하여 개체번호, 생산지, 생산자 등 다양한 정보를 표시함으로써 축산물의 신뢰성을 높이고 있다. 최근 들어 한우육의 경우에도 수량적인 측면보다는 축산물의 안정성과 품질, 제품을 구매하는 데 있어서 제품의 진위성 등에 초점이 맞춰지고 있다. 즉, 소비자는 광우병과 같이 사람과 소가 공통으로 가지는 질병을 차단시켜 안전성이 입증된 소고기를 구매하려 한다. As interest in health increases, consumers' demand for meat is also shifting from simply quantitative to stability and quality, and in particular, the place of origin (brand) and the reliability of the distribution process from production to consumption are considered important. In response to this change, the European Union has implemented a production history system for the distribution of beef, displaying a variety of information such as individual number, place of origin, and producers to increase the reliability of livestock products. Recently, in the case of Korean beef, the focus has been on the stability and quality of livestock products, and on the authenticity of products in purchasing products, rather than the quantity. In other words, consumers try to buy beef that is proven safe by blocking diseases common to humans and cows, such as mad cow disease.

한편, 우리나라 한우산업은 외국산 소고기의 수입개방에 따른 위기감으로 농가들의 산업적 기반이 위협받고 있다. 이러한 현상은 FTA 체결로 인한 값싼 미국 산 소고기가 수입됨에 따라 가격면에서 이들과의 경쟁이 불가능하다는 사실에 근거를 두고 있다. 이로 인해 한우가 가격 경쟁력을 갖추고 안전성을 확보하여 시장에서 살아남기 위해 원산지표시제와 생산이력제를 적극적으로 도입하려는 추세이다. Meanwhile, the Korean beef industry is threatening the industrial base of farmers due to the crisis caused by the import and opening of foreign beef. This is based on the fact that the price of US beef from the FTA is not enough to compete with them as imports are made. As a result, Hanwoo is actively trying to introduce a labeling system of origin and a production history system in order to survive in the market by securing price competitiveness and safety.

최근 식량산업에서는 원산지표시(originality)와 함께 생산이력체계(traceability)가 국가, 지역, 농가에 따라 농산물을 차별화할 수 있는 중요한 관리방법으로 제시되고 있으며 일부 선진국에서는 이미 관련 기술을 실제 농산물 관리에 도입하여 활용하고 있다. 생산이력체계는 계속적으로 개체별 송아지의 성장과정에서 일어나는 능력, 관리, 질병 등에 대한 기록이 이뤄지고 도축·가공 과정과 소비자들에게 유통되는 과정에도 최초 송아지에 부여했던 고유번호(이표번호)가 유지되어 출생에서 소비자 식탁까지 전과정이 전산시스템으로 운영되는 컴퓨터정보기술을 기반으로 하는 체계이다. 그러나 이와 같은 생산이력체계는 소의 고유번호가 가진 정보에만 의존하기 때문에 고유번호가 오기/유실된 경우 추적이 불가능하다는 단점이 있다. 따라서, 생산이력체계가 효과적으로 작동하기 위해서는 생명공학기술을 접목시킬 필요가 제기되었고, 기존에는 혈액형 및 생화학적인 지표의 다형성 분석방법을 사용하였으나 이런 기법들은 분석을 위해 사용되는 조직들이 한계가 있다는 단점을 가지고 있다. 더구나 소에 있어서 혈액형 검사는 정확한 부계혈통을 결정하지 못하는 단점이 있다. 이로 인해 최근 들어 DNA 다형성에 기초한 다양한 유전적 분석 방법들이 개발되었으며, 이를 이용하여 품종간 및 개체간의 동정이 이루어지고 있다. 최근의 생산이력체계는 소 DNA 마커를 이용한 유전자 감식기법이 활용되고 있다. DNA 마커는 MS(microsatellite)와 SNP(single nucleotide polymorphism)로 나눌 수 있으며, 현재 MS 마커(ISAG, International Society for Animal Genetics)가 소 유전자 감식을 위해 주로 이용되고 있다.In recent years, the food industry, along with the originality and traceability, has been suggested as an important management method for differentiating agricultural products by country, region, and farm. Some developed countries have already introduced related technologies to actual agricultural product management. Is utilized. The production history system keeps records of the ability, management, disease, etc. that occur during the growth of individual calves, and maintains the unique number assigned to the first calf during slaughter and processing and distribution to consumers. It is a system based on computer information technology in which the entire process from birth to consumer table is operated by computer system. However, such a production history system has a disadvantage in that it is impossible to trace when a unique number comes in or is lost because it depends only on the information of the cow's unique number. Therefore, in order for the production history system to work effectively, it is necessary to combine biotechnological techniques, and in the past, polymorphic analysis methods of blood types and biochemical indicators have been used, but these techniques have limitations in that the tissues used for analysis are limited. Have. Moreover, blood tests in cattle have the disadvantage of not determining the exact paternal lineage. As a result, recently, various genetic analysis methods based on DNA polymorphism have been developed, and identification between breeds and individuals is made using them. Recent production history systems are using genetic identification techniques using small DNA markers. DNA markers can be divided into MS (microsatellite) and single nucleotide polymorphism (SNP), and MS markers (ISAG, International Society for Animal Genetics) are mainly used for small gene identification.

소의 유전체 중 40% 정도의 염기서열이 반복적으로 이루어지는 MS DNA의 반복염기서열로 밝혀져 이러한 반복염기서열에 대한 연구가 많이 이루어지고 있다. MS는 단순서열반복(simple sequence repeats; SSRs), 짧은 탠덤 반복(short tandem repeats; STRs) 또는 단순서열길이다형성(simple sequence length polymorphisms; SSLPs)으로도 불리며 이들은 2 내지 6 개 정도의 염기서열이 반복되는 DNA군으로 게놈 내에 골고루 분포하고 매우 높은 다형성을 나타내는 비암호화 DNA 서열에 해당한다(Zajc I and Sampson J., J Hered 90:104-107, 1999). MS 변이는 반복 단위(repeat units)의 수에 있으며 이들 반복 단위 수의 차이는 DNA 복제 시 일어난 '슬리피지(slippage)'에 의한 것으로 알려져 있다. 즉, DNA 중합효소(polymerase)가 반복 영역(repeat region)을 복사할 때 '슬리피지'가 일어남으로써 반복(repeat)의 수를 바꾸게 된다. 다양한 반복의 수는 이러한 슬리피지가 반복된 결과로 볼 수 있으며 이외에 감수분열시 불균등한 교차(unequal crossing)에 의해서도 일어날 수 있다고 한다. 특정 좌위(loci)에서 반복단위의 반복수를 따라 개체간의 다양성이 인정되는데(Koreth J, O'Leary JJ and McGee, J Pathol 179:239-248, 1996), 반복수에 품종간 다형이 있는 경우에 인접영역에 설계한 프라이머를 이용하여 중합효소연쇄반응(Polymerase Chain Reaction; PCR)을 행하면, PCR 산물 길이에 다형이 관찰되고, DNA 다형을 검출하는 것이 가능해진다. MS를 이용한 다형검출 마커는 MS 마커라고 불리우고 있다(O. Parnaud, X. Chen, S. R. McCouch, Mol. Gen. Genet. 252:597-607, 1996). 현재 소에서 약 천 개의 MS 좌위가 밝혀져 있으며, 이들의 특성 및 염색체상의 위치파악이 이루어져 왔다. 이들 중 품종간, 개체간 다형성이 높은 MS 마커들을 선별하여 혈통검정을 위한 수단으로서 사용하고 있다.It is found that the repeat base sequence of MS DNA, which is about 40% of the nucleotide sequence of the bovine genome, has been studied a lot of such repeat base sequence. MS is also called simple sequence repeats (SSRs), short tandem repeats (STRs), or simple sequence length polymorphisms (SSLPs), which repeat two to six sequences. And a non-coding DNA sequence that is evenly distributed in the genome and exhibits a very high polymorphism (Zajc I and Sampson J., J Hered 90: 104-107, 1999). MS variation is in the number of repeat units and the difference in the number of repeat units is known to be due to the 'slippage' that occurs during DNA replication. That is, when a DNA polymerase copies a repeat region, the slippage occurs, thereby changing the number of repeats. The number of different repetitions can be seen as a result of this slippage being repeated and can also be caused by unequal crossings during meiosis. Diversity is recognized between individuals according to the number of repeat units in a particular loci (Koreth J, O'Leary JJ and McGee, J Pathol 179: 239-248, 1996). When polymerase chain reaction (PCR) is carried out using a primer designed in an adjacent region, polymorphisms are observed in the length of the PCR product and DNA polymorphism can be detected. Polymorphism detection markers using MS are called MS markers (O. Parnaud, X. Chen, SR McCouch, Mol. Gen. Genet. 252: 597-607, 1996). At present, about a thousand MS loci have been identified in cattle, and their properties and chromosome localization have been established. Among these, MS markers with high polymorphism between varieties and individuals are selected and used as a means for lineage assay.

그러나, 현재 주로 이용되는 MS 마커는 분석조건(기기, 시약, 실험자 등)에 따라 결과가 일정치 않아 사후에 한우개체인식 분석을 할 경우 결과가 부정확할 가능성이 매우 높으며, 검사비용이 고가인 단점이 있다. However, currently used MS markers are inconsistent depending on the analysis conditions (instrument, reagents, experimenters, etc.). Therefore, the results may be inaccurate when Hanwoo individual recognition is performed after the analysis. There is this.

이에 본 발명에서는 유전자 지노타이핑 실험의 재현성이 뛰어나고 분석이 용이한 SNP를 이용한 유전자 세트를 개발하였다. SNP(Single Nucleotide Polymorphism)란 인종간 또는 연령간, 성별간, 염색체가 갖고 있는 염기 서열 중 개인 편차를 나타내는 염기변이를 말하여, 한 염기쌍(single base-pair variation)의 차이로 DNA 서열 다형성 중에서 가장 많이 존재한다. SNP 유전자는 기존의 마커들에 비해 보다 촘촘하고 안정한 맵을 작성할 수 있다는 점과 새로운 기술, 특히 DNA 칩을 포함하는 고효율기술(high-throughput technology)에 보다 적합한 특성, 즉 탐색에서 분석에 이르는 전 과정이 컴퓨터에 의해 고속으로 자동화될 수 있다는 점이 SNP의 차별화된 특성이라고 할 수 있다. 예를 들면, SNP 유전자는 고효율 지노타이핑(high-throughput genotyping)에 매우 유용하다. Accordingly, the present invention has developed a gene set using SNP that is excellent in reproducibility of gene genotyping experiments and easy to analyze. Single Nucleotide Polymorphism (SNP) refers to a nucleotide variation that represents individual variation among nucleotide sequences among races, ages, sexes, and chromosomes.It is the difference between single base-pair variation and the most among DNA sequence polymorphisms. Many exist. SNP genes are more dense and stable than conventional markers and are more suitable for new technologies, especially high-throughput technologies including DNA chips, the entire process from search to analysis. The ability to be automated at high speed by this computer is a distinguishing feature of SNPs. For example, the SNP gene is very useful for high-throughput genotyping.

본 발명에서는, SNP 데이터베이스에서 선발된 SNP를 Golden-Gate Assay (Illumina, CA, USA) 방법을 이용하여 지노타이핑을 수행하였다. Golden Gate Assay는 연구자가 원하는 SNP를 96-에서 1536-플렉스(plex)까지 선발제작하여 지노 타이핑할 수 있으며 모든 SNP는 하나의 OPA(Oligo Pool All)로 구성된다. 대립유전자 특이적 프라이머 연장(Allele Specific Primer Extension) 방법으로 증폭된 올리고들을 비드에 혼성화(Hybridization)시키고 형광스캐너인 비드익스프레스 리더(BeadXpress Reader)를 통해 읽어진 SNP 각각의 강도 데이터(Intensity Data)는 비드스튜디오 소프트웨어(BeadStudio Software)를 이용하여 자동으로 유전자형이 결정된다. Golden Gate Assay 결과는 BeadStudio Software를 이용하여 복제수 변이(Copy Number Variation), 이형접합소실(Loss Of Heterozygosity)을 동시에 분석할 수 있으며, HelixTree 등의 분석 소프트웨어를 이용하여 전체 게놈 연관 분석(Whole Genome Association Analysis)을 단시간 내에 수행한다. In the present invention, genotyping was performed on the SNP selected from the SNP database using the Golden-Gate Assay (Illumina, CA, USA) method. Golden Gate Assay can genotype the SNPs the researchers want from 96- to 1536-plexes, and all SNPs consist of one OPA (Oligo Pool All). Oligos amplified by the Allele Specific Primer Extension method were hybridized to the beads, and the intensity data of each SNP read through the fluorescence scanner, BeadXpress Reader, was added to the beads. Genotyping is done automatically using Studio Studio Software. Golden Gate Assay results can be analyzed simultaneously with Copy Number Variation and Loss of Heterozygosity using BeadStudio Software and Whole Genome Association using analysis software such as HelixTree. Analysis) is performed in a short time.

본 발명은 종모우를 포함하여 전국적으로 고르게 분포하는 다수의 한우집단 (570 두)에서 각각의 한우개체를 구별해 낼 수 있는 SNP 유전자 개발을 목표로 하였으며, 개발된 SNP 유전자를 이용하여 한우 개체인식 및/또는 혈통확인이 가능함을 보였다. 구체적으로, 본 발명은 선발된 소 SNP를 Golden-Gate Assay를 이용하여 지노타이핑하고 그 결과 마이너 대립유전자 빈도(minor allele frequency; MAF)가 0.3 이상인 SNP 유전자 세트 384개를 선발하여 한우 개체인식 및/또는 혈통확인에 성공적으로 이용함으로써 본 발명을 완성하였다. The present invention aims to develop SNP genes that can distinguish each Korean cattle individual from a number of Korean cattle groups (570), including even cattle, nationwide. And / or pedigree identification was possible. Specifically, the present invention genotypes selected bovine SNPs using a Golden-Gate Assay. As a result, 384 SNP gene sets having a minor allele frequency (MAF) of 0.3 or more are selected to recognize Hanwoo individuals and / or Alternatively, the present invention has been completed by successful use in lineage identification.

본 발명의 목적은 한우의 개체인식 및/또는 혈통확인을 위한 단일염기다형성(SNP) 유전자 세트를 제공하는 것이다. It is an object of the present invention to provide a single nucleotide polymorphism (SNP) gene set for individual identification and / or pedigree identification of Hanwoo.

또한, 본 발명의 다른 목적은 상기 SNP 유전자 세트를 포함하는 폴리뉴클레오티드 또는 이것의 상보적 폴리뉴클레오티드를 포함하는, 한우의 개체인식 또는 혈통확인을 위한 폴리뉴클레오티드를 제공하는 것이다. In addition, another object of the present invention is to provide a polynucleotide for identification or lineage of Hanwoo, including a polynucleotide comprising the SNP gene set or a complementary polynucleotide thereof.

또한, 본 발명의 다른 목적은 상기 폴리뉴클레오티드 또는 이것의 상보적 폴리뉴클레오티드와 혼성화하는 폴리뉴클레오티드를 제공하는 것이다. It is another object of the present invention to provide a polynucleotide that hybridizes with the polynucleotide or its complementary polynucleotide.

또한, 본 발명의 다른 목적은 상기 폴리뉴클레오티드를 포함하는 한우의 개체인식 또는 혈통확인을 위한 키트를 제공하는 것이다. In addition, another object of the present invention to provide a kit for identification or pedigree identification of Hanwoo including the polynucleotide.

또한, 본 발명의 다른 목적은 상기 SNP 유전자 세트를 사용한 한우의 개체인식 방법을 제공하는 것이다. In addition, another object of the present invention is to provide an individual recognition method of Hanwoo using the SNP gene set.

또한, 본 발명의 다른 목적은 상기 SNP 유전자 세트를 사용한 한우의 혈통확인 방법을 제공하는 것이다. In addition, another object of the present invention is to provide a method for identifying pedigree of Hanwoo using the SNP gene set.

또한, 본 발명의 다른 목적은 상기 SNP 유전자 세트를 사용한 한우의 원산지 판별 방법을 제공하는 것이다. In addition, another object of the present invention is to provide a method for determining the origin of Hanwoo using the SNP gene set.

본 발명의 목적을 달성하기 위하여, 본 발명은 한우의 개체인식 및/또는 혈통확인을 위한 SNP 유전자 세트를 제공한다. In order to achieve the object of the present invention, the present invention provides a set of SNP gene for the identification and / or lineage identification of Hanwoo.

본 발명의 목적을 달성하기 위하여, 본 발명은 한우의 개체인식 및/또는 혈통확인을 위한, 단일염기변이를 갖는 서열번호 1 내지 384로 구성된 군으로부터 선택된 2개 이상의 SNP(single nucleotide polymorphism) 유전자 세트를 제공한다. 서열번호 1 내지 384의 SNP 유전자 세트는 Golden-Gate Assay 방법을 이용하여 지노타이핑한 SNP 중 SNP의 MAF가 0.3 이상인 것을 선발한 것이다. 표 2의 MAF 0.3 이상의 SNP는 메이저 대립유전자(major allele) 뿐만 아니라 마이너 대립유전자(minor allele)의 출현빈도가 높아 한우 개체확인을 위한 유전적 다양성이 충분하기 때문이다. In order to achieve the object of the present invention, the present invention provides a set of two or more single nucleotide polymorphism (SNP) genes selected from the group consisting of SEQ ID NOs: 1 to 384 having a single base mutation for the identification and / or pedigree of Hanwoo To provide. The SNP gene set of SEQ ID NOS: 1 to 384 was selected from the genotyped SNPs with SNP MAF of 0.3 or more using the Golden-Gate Assay method. SNPs with a MAF of 0.3 or higher in Table 2 have a high frequency of occurrence of major alleles as well as minor alleles, which is sufficient for genetic diversity for identification of Hanwoo cattle.

본 발명의 다른 목적을 달성하기 위하여, 본 발명은 서열번호 1 내지 384 중 어느 하나의 염기변이 부위를 포함하고 10개 이상 50개 이하의 연속 염기로 구성된 폴리뉴클레오티드 또는 이것의 상보적 폴리뉴클레오티드를 포함하는, 한우 개체인식 또는 혈통확인을 위한 폴리뉴클레오티드를 제공한다. In order to achieve another object of the present invention, the present invention comprises a polynucleotide comprising a base mutation site of any one of SEQ ID NOs: 1 to 384 and composed of 10 or more and 50 or less consecutive bases or a complementary polynucleotide thereof. To provide a polynucleotide for Hanwoo individual recognition or pedigree.

본 발명의 다른 목적을 달성하기 위하여, 본 발명은 상기 폴리뉴클레오티드 또는 이것의 상보적 폴리뉴클레오티드와 혼성화하는 폴리뉴클레오티드를 제공한다. In order to achieve another object of the present invention, the present invention provides a polynucleotide that hybridizes with the polynucleotide or its complementary polynucleotide.

본 발명의 다른 목적을 달성하기 위하여, 본 발명은 상기 폴리뉴클레오티드를 포함하는 한우 개체인식 또는 혈통확인을 위한 키트를 제공한다. In order to achieve another object of the present invention, the present invention provides a kit for Hanwoo individual recognition or pedigree including the polynucleotide.

본 발명의 다른 목적을 달성하기 위하여, 본 발명은 단일염기변이를 갖는 서열번호 1 내지 384로 구성된 군으로부터 선택된 2개 이상의 SNP(single nucleotide polymorphism) 유전자 세트를 사용한 한우 개체인식 방법을 제공한다. In order to achieve another object of the present invention, the present invention provides a method for recognizing Korean cattle using two or more single nucleotide polymorphism (SNP) gene sets selected from the group consisting of SEQ ID NOs: 1 to 384 having a single base mutation.

본 발명의 다른 목적을 달성하기 위하여, 본 발명은 i) 피검 소로부터 DNA를 추출하는 단계; ii) 서열번호 1 내지 384 중 어느 하나의 염기변이 부위, 또는 이 부위 염기와 염기쌍을 이루는 상보사슬에서의 염기부위를 포함하는 DNA를 증폭시키는 단계; iii) 서열번호 1 내지 384 중 어느 하나의 염기변이 부위, 또는 이 부위 염기와 염기쌍을 이루는 상보사슬에서의 염기에 인접한 1 염기 부위까지의 서열에 상보적인 프라이머를 합성하는 단계; iv) 시퀀서(sequencer)를 이용하여 상기 iii)에서 반응에 사용된 염기 종류를 판정하는 단계; 및 v) 단계 iv)에서 판정된 염기 종류를 대조군과 비교하는 단계를 포함하는, 서열번호 1 내지 384로 구성된 군으로부터 선택된 2개 이상의 SNP(single nucleotide polymorphism) 유전자 세트를 사용한 한우 개체인식 방법을 제공한다. 피검 소 DNA 시료별 본 발명의 384개의 SNP 유전자 세트의 각 지노타입(AA, AB, BB 형)을 비교하였을 때 384개의 SNP 유전자가 서로 100% 일치하는 경우 동일 개체, 100% 일치하지 않는 경우 독립된 개체로 판단한다. In order to achieve another object of the present invention, the present invention comprises the steps of i) extracting DNA from the test subject; ii) amplifying a DNA comprising a base mutation region of any one of SEQ ID NOs: 1 to 384, or a base region in the complementary chain base paired with the region base; iii) synthesizing a primer complementary to the sequence of any one of SEQ ID NOs: 1 to 384, or up to one base site adjacent to the base in the complementary chain base paired with this site base; iv) determining the type of base used in the reaction in iii) using a sequencer; And v) comparing the base type determined in step iv) with a control group, providing a method for recognizing Korean cattle using two or more single nucleotide polymorphism (SNP) gene sets selected from the group consisting of SEQ ID NOs: 1 to 384. do. Comparing each genotype (type AA, AB, BB) of the 384 SNP gene sets of the present invention by test DNA samples, the same individuals were found to be 100% identical to each other, and the same individuals were not 100% identical. Judging by the individual.

본 발명의 다른 목적을 달성하기 위하여, 본 발명은 단일염기변이를 갖는 서열번호 1 내지 384로 구성된 군으로부터 선택된 2개 이상의 SNP(single nucleotide polymorphism) 유전자 세트를 사용한 한우 혈통확인 방법을 제공한다. In order to achieve another object of the present invention, the present invention provides a method for identifying Korean cattle lineage using two or more single nucleotide polymorphism (SNP) gene sets selected from the group consisting of SEQ ID NOs: 1 to 384 having a single base mutation.

본 발명의 다른 목적을 달성하기 위하여, 본 발명은 i) 피검 소로부터 DNA를 추출하는 단계; ii) 서열번호 1 내지 384 중 어느 하나의 염기변이 부위, 또는 이 부위 염기와 염기쌍을 이루는 상보사슬에서의 염기부위를 포함하는 DNA를 증폭시키는 단계; iii) 서열번호 1 내지 384 중 어느 하나의 염기변이 부위, 또는 이 부위 염기와 염기쌍을 이루는 상보사슬에서의 염기에 인접한 1 염기 부위까지의 서열에 상보적인 프라이머를 합성하는 단계; iv) 시퀀서(sequencer)를 이용하여 상기 iii)에서 반응에 사용된 염기 종류를 판정하는 단계; 및 v) 단계 iv)에서 판정된 염기 종류를 대조군과 비교하는 단계를 포함하는, 서열번호 1 내지 384로 구성된 군으로부터 선택된 2개 이상의 SNP(single nucleotide polymorphism) 유전자 세트를 사용한 한우 혈통확인 방법을 제공한다. 종모우와 대립유전자(A, B 형) 중 1 개가 후대소와 일치하는 경우 그 대립유전자는 종모우로부터 유전된 것으로 판단하고, 본 발명의 384개의 SNP 유전자 모두에서 일치하는 경우 그 대립유전자는 혈통관계가 성립된 것으로 판단한다. In order to achieve another object of the present invention, the present invention comprises the steps of i) extracting DNA from the test subject; ii) amplifying a DNA comprising a base mutation region of any one of SEQ ID NOs: 1 to 384, or a base region in the complementary chain base paired with the region base; iii) synthesizing a primer complementary to the sequence of any one of SEQ ID NOs: 1 to 384, or up to one base site adjacent to the base in the complementary chain base paired with this site base; iv) determining the type of base used in the reaction in iii) using a sequencer; And v) comparing the base type determined in step iv) with a control group, wherein the method provides a method for identifying Korean cattle lineage using two or more single nucleotide polymorphism (SNP) gene sets selected from the group consisting of SEQ ID NOs: 1 to 384. do. If one of the cows and alleles (types A and B) is consistent with later generations, the allele is determined to be inherited from the cows, and if all 384 SNP genes of the present invention are matched, the alleles have a lineage relationship. It is judged to be established.

본 발명의 다른 목적을 달성하기 위하여, 본 발명은 단일염기변이를 갖는 서열번호 1 내지 384로 구성된 군으로부터 선택된 2개 이상의 SNP(single nucleotide polymorphism) 유전자 세트를 사용한 한우 원산지 판별 방법을 제공한다. In order to achieve another object of the present invention, the present invention provides a method for determining the origin of Korean beef using two or more single nucleotide polymorphism (SNP) gene set selected from the group consisting of SEQ ID NOs: 1 to 384 having a single base mutation.

본 발명은 한우 개체인식 및/또는 혈통확인을 위한 SNP 유전자 세트를 제공하고, 상기 SNP 유전자 세트를 이용한 한우 개체인식 방법, 혈통확인 방법, 원산지 판별 방법을 제공하며, 이는 한우생산이력체계의 유전자 검사방법으로 적용가능하다.The present invention provides a SNP gene set for Hanwoo individual recognition and / or pedigree identification, and provides a Hanwoo individual recognition method, a pedigree identification method, and a country of origin determination method using the SNP gene set, which is a genetic test of the Hanwoo production history system. Applicable in a way.

이하, 본 발명의 구성요소와 기술적 특징을 다음의 비제한적인 실시예들을 통하여 보다 상세하게 설명하고자 한다. 그러나 하기의 실시예들은 본 발명을 상세하게 설명하기 위한 것일 뿐, 본 발명의 구성요소의 기술적 범위를 실시예들에 예시한 것들로 한정하고자 하는 것은 아니다. 본 발명에서 인용된 문헌은 본 발명의 명세서에 참조로서 통합된다. Hereinafter, the components and technical features of the present invention will be described in more detail with reference to the following non-limiting examples. However, the following embodiments are only intended to describe the present invention in detail, and are not intended to limit the technical scope of the components of the present invention to those illustrated in the embodiments. The documents cited in the present invention are incorporated herein by reference.

실시예Example

실시예Example 1: SNP 유전자 개발 및 검증용 시료 1: Sample for SNP Gene Development and Validation

농협젖소개량사업부의 한우 종모우 57 두, 축협서울공판장에서 한우 후대송아지 139 두 및 한우육우 374 두 등 총 570 두가 SNP 유전자 개발 및 검증 실험에 이용되었다 (표 1). A total of 570 dogs were used for the development and validation of SNP genes, including 57 cows of the Hanwoo dairy cattle division and 139 cows of the Korean cattle, and 374 cows of the cattle.

SNP 유전자 개발 및 검증용 시료 현황Sample Status for SNP Gene Development and Validation 한우 Hanwoo 개체수 (두)Number of objects (two) 시료확보기관Sample Tube 한우종모우Hanwoo cattle 5757 농협젖소개량사업소Nonghyup Milk Introduction Office 한우후대소Korean beef cattle 139139 농협서울공판장Nonghyup Seoul Fair 한우거세육우Korean beef cattle 216216 농협서울공판장Nonghyup Seoul Fair 한우육우(수)Korean Beef Beef 7979 농협서울공판장Nonghyup Seoul Fair 한우육우(암)Korean beef cattle (cancer) 7979 농협서울공판장Nonghyup Seoul Fair 총 개체수 (두)Total population (two) 570570

1-1. DNA 시료의 준비1-1. Preparation of DNA Samples

소 검체에서 DNA를 추출하기 위해 0.5g의 검체 또는 500 의 혈액을 1.5 튜브에 옮겼다. 핵산 해리 혼합액을 300 넣고 단백질분해효소 K (20/)를 10 첨가하였다. 잘 혼합한 후 검체가 완전히 용해될 때까지 55 항온수조에 두었다 (약 40분 내지 1시간 30분). 이때 10분 간격으로 약 10 내지 20초 간 충분히 혼합하였다. 얼음 위에 3분 간 둔 뒤 단백질 침전 용액을 100 첨가한 후 20초 간 충분히 혼합하였다. 얼음 위에 5분 간 둔 뒤 14,000 rpm에서 5분 간 원심분리하였다. 상층액을 새로운 1.5 튜브에 옮기고 이소프로판올 300를 첨가하여 위 아래로 뒤집으며 잘 섞어준 후 14,000 rpm에서 5분간 원심분리하였다. 상층액을 피펫으로 DNA 응축물이 없어지지 않도록 조심스럽게 버렸다. 70% 에탄올을 1 넣고 2~3번 위 아래로 뒤집으며 잘 섞은 뒤 14,000 rpm에서 5분간 원심분리하였다. 상층액을 피펫을 사용하여 DNA 응축물이 없어지지 않도록 조심스럽게 제거하고 상온에서 DNA 응축물을 공기 중에서 자연 건조시켰다 (약 5분간 상온에 방치). RNA 분해효소가 첨가된 DNA 재수화 용액 (RNA 분해효소 40 ng/)을 가한 후 튜브 끝을 손가락으로 가볍게 치면서 DNA 응축물을 녹였다.0.5 g of sample or 500 blood were transferred to 1.5 tubes to extract DNA from bovine samples. 300 nucleic acid dissociation mixtures were added and 10 protease K (20 /) was added. Mix well and place in 55 constant temperature water bath until the sample is completely dissolved (about 40 minutes to 1 hour 30 minutes). At this time, the mixture was sufficiently mixed for about 10 to 20 seconds at 10 minute intervals. After 3 minutes on ice, 100 was added to the protein precipitation solution and mixed well for 20 seconds. After 5 minutes on ice it was centrifuged for 5 minutes at 14,000 rpm. The supernatant was transferred to a new 1.5 tube, added with isopropanol 300, upside down and mixed well, followed by centrifugation at 14,000 rpm for 5 minutes. The supernatant was pipetted carefully to ensure that no DNA condensate was lost. 1 70% ethanol was added upside down 2 ~ 3 times and mixed well and centrifuged for 5 minutes at 14,000 rpm. The supernatant was carefully removed using a pipette to prevent DNA condensation from disappearing, and the DNA condensate was naturally dried in air at room temperature (left at room temperature for about 5 minutes). DNA rehydration solution (RNA lyase 40 ng /) was added to dissolve DNA condensate by tapping the end of the tube with a finger.

실시예Example 2: 후보 SNP 선발 및  2: candidate SNP selection and 지노타이핑Zino Typing ( ( genotypinggenotyping ))

NCBI 소(Bovine) SNP 데이터베이스(http://ftp.hgsc.bcm.tmc.edu/pub/data/Btaurus/snp/Btau20040927/bovine-snp.txt)와 AFNS 소(Bovine) SNP 데이터베이스(http://www.afns.ualberta.ca/Hosted/Bovine%20Genomics/SNP_WEB.xls)(University of Alberta)에 등록되어있는 SNP 가운데 대립유전자빈도(allele frequency) 정보를 가진 SNP 3,072개를 선발하였다. NCBI Bovine SNP Database (http://ftp.hgsc.bcm.tmc.edu/pub/data/Btaurus/snp/Btau20040927/bovine-snp.txt) and AFNS Bovine SNP Database (http: / Among SNPs registered at /www.afns.ualberta.ca/Hosted/Bovine%20Genomics/SNP_WEB.xls (University of Alberta), 3,072 SNPs with allele frequency information were selected.

선발된 3,072개의 SNP를 Golden-Gate Assay (Illumina, CA, USA) 방법을 이용하여 지노타이핑(genotyping)하였다. Golden-Gate Genotyping Assay (BeadXpress)는 SNP 지노타이핑(genotyping)을 위한 베라코드 기술(Veracode Technology)로서 모든 SNP가 하나의 OPA(Olig Pool All)로 구성되며, 대립유전자 특이적 프라이머 신장(Allele Specific Primer Extension) 방법으로 증폭된 올리고들을 비드에 혼성화(hibridization)시키고 형광스캐너인 비드익스프레스 리더(BeadXpress Reader)를 통해 읽어진 SNP 각각의 강도 데이터(intensity data)는 비드스튜디오 소프트웨어(BeadStudio Software)를 이용하여 자동으로 지노타입(genotype)이 결정된다. Golden-Gate Assay는 단일염기연장(Single Base Extension)이나 Taqman 방법으로 실험가능한 규모(100개 이하 SNP) 이상의 SNP를 지노타이핑하기 위한 최적의 방법으로서, 본 발명에 사용하였다.Selected 3,072 SNPs were genotyped using the Golden-Gate Assay (Illumina, Calif., USA) method. Golden-Gate Genotyping Assay (BeadXpress) is Veracode Technology for SNP genotyping.All SNPs are composed of one OPA (Olig Pool All) and Allele Specific Primer. The oligos amplified by the extension method are hybridized to the beads, and the intensity data of each SNP read through the fluorescence scanner, BeadXpress Reader, is automatically generated by using the BeadStudio Software. The genotype is determined. Golden-Gate Assay was used in the present invention as an optimal method for genotyping SNPs larger than the scale (up to 100 SNPs) that can be tested by the Single Base Extension or Taqman method.

보다 구체적으로 설명하면 다음과 같다. DNA 플레이트에 5의 DNA 플레이트 제조용 시약을 넣고 DNA 시료(50 ng/㎕)를 옮겨 넣었다. DNA 플레이트를 가열기로 봉합하고 90℃로 예열시켜놓은 가열기에서 30분간 반응시켰다. 가열 봉합재를 용액이 튀지 않도록 조심스럽게 제거한 후 5㎕의 침전시약을 넣고 용액이 청색으로 변할 때까지 충분히 혼합하였다. 15㎕의 2-프로판올를 넣고 3000xg로 20분간 원심분리한 후 상층액을 제거하고 상온에서 15~20분간 건조시켰다. DNA 플레이트의 각 칸에 10㎕의 혼탁액을 넣고 청색의 침전물이 완전히 풀어질 때까지 충분히 혼합하였다.More specifically described as follows. The DNA plate preparation reagent of 5 was put into a DNA plate, and a DNA sample (50 ng / μl) was transferred. The DNA plate was sealed with a heater and reacted for 30 minutes in a heater preheated to 90 ° C. The heat sealant was carefully removed to prevent the solution from splashing, and then 5 µl of precipitation reagent was added and mixed well until the solution turned blue. 15 μl of 2-propanol was added, centrifuged at 3000 × g for 20 minutes, the supernatant was removed, and dried at room temperature for 15 to 20 minutes. 10 μl of the turbidity solution was added to each compartment of the DNA plate and mixed well until the blue precipitate was completely released.

유전형특이연장반응용 플레이트 각 칸에 10㎕의 올리고시약을 넣고 30㎕의 올리고 혼성 접합시약을 추가로 넣었다. DNA 플레이트의 봉합을 조심스럽게 제거하고 10㎕의 DNA 반응액을 유전형특이연장반응용 플레이트로 옮기고 비드가 완전히 풀어질 때까지 충분히 혼합하였다. 70℃로 예열된 가열기에 유전형특이연장반응용 플레이트를 놓고, 즉시 가열기를 30℃로 맞춘 후 30℃로 온도가 떨어질 때까지 놓아두었다.10 μl of oligo reagent was added to each compartment of the genotype-specific extension reaction plate, and 30 μl of oligo hybrid reagent was further added. The suture of the DNA plate was carefully removed and 10 μl of DNA reaction solution was transferred to the genotyping extension plate and mixed well until the beads were completely released. The plate for the genotype-specific extension reaction was placed in a heater preheated to 70 ° C., immediately set the heater to 30 ° C., and then left at 30 ° C. until the temperature dropped.

2분 동안 또는 비드가 한 곳으로 모일 때까지 자석 플레이트 위에 유전형특이연장반응용 플레이트를 올려놓고 비드를 제외한 상층액(약 50㎕)을 제거하고 50㎕의 연장접합반응 혼합용액을 넣었다. 비드가 완전히 풀어질 때까지 충분히 혼합하였다. 다시 자석 플레이트 위에 유전형특이연장반응용 플레이트를 올려놓고 비드를 제외하고 연장접합반응 혼합용액을 제거하였다. 이 과정을 1회 반복하였다. 8-채널 피펫을 사용하여 50㎕의 범용완충액을 유전형특이연장반응용 플레이트의 각 칸에 넣고 2분 동안 비드가 한 곳으로 모일 때까지 자석 플레이트 위에 유전형특이연장반응용 플레이트를 올려놓았다. 8-채널 피펫을 사용하여 비드를 제외하고 범용완충액을 제거하였다. 이 과정을 1회 반복하였다. 8-채널 피펫을 사용하여 37㎕ 연장접합반응 효소를 유전형특이연장반응용 플레이트의 각 칸에 넣어주고 플레이트를 접착 필름으로 봉합한 다음 비드가 풀어지도록 1,600~1,700 rpm으로 비드가 완전히 풀어질 때까지 1분간 충분히 혼합하였다. 유전형특이연장반응용 플레이트를 45℃로 예열시킨 가열기에서 15분간 반응시켰다.The plate for genotype-specific extension reaction was placed on the magnet plate for 2 minutes or until the beads were gathered in one place. The supernatant except the beads (about 50 µl) was removed, and 50 µl of extension conjugated reaction solution was added thereto. Mix well until the beads are completely released. Again, the plate for the genotype-specific extension reaction was placed on the magnet plate and the extension bonding reaction solution was removed except for the beads. This process was repeated once. Using an 8-channel pipette, 50 μl of the universal buffer solution was placed in each compartment of the genotyping-specific extension plate, and the genotyping extension plate was placed on the magnetic plate until the beads gathered in one place for 2 minutes. An 8-channel pipette was used to remove the universal buffer except the beads. This process was repeated once. Using an 8-channel pipette, add 37 μl extension enzyme into each compartment of the genotyping-specific extension plate, seal the plate with an adhesive film, and allow the beads to fully release at 1600 to 1,700 rpm to release the beads. Mix well for 1 minute. The plate for genotype-specific extension reaction was reacted for 15 minutes in a heater preheated to 45 ° C.

PCR 혼합용액 튜브에 64㎕의 DNA 중합효소와 50㎕의 우라실 DNA 글루코실라제(Uracil DNA glucosylase)를 넣고 충분히 혼합하여 PCR 플레이트에 PCR 혼합액을 30㎕씩 분주하였다. 2분 동안 또는 비드가 한곳으로 몰릴 때까지 자석 플레이트 위에 유전형특이연장반응용 플레이트를 올려놓고 비드를 제외한 상층액(약 50㎕)를 제거한 후 50㎕의 범용완충액을 넣었다. 다시 비드가 모일 수 있도록 자석 플레이트 위에 2분 동안 올려 놓고 상층액을 모두 제거하고 35㎕의 DNA 해리용액을 유전형특이연장반응용 플레이트의 각 칸에 넣어주었다. 비드를 풀어주기 위해 1,800-1,900 rpm으로 1분간 충분히 혼합하고 95℃로 예열시킨 가열기에 1분 동안 올려놓았다. 가열기에서 플레이트를 꺼낸 뒤 2분 동안 또는 비드가 모일 때까지 자석 플레이트 위에 플레이트를 올려놓은 후 30㎕ 상층액을 PCR 플레이트로 옮겼다. PCR 플레이트를 가열 봉합하고 유전자 증폭 장비로 즉시 옮겼다. PCR 반응의 조건은 다음과 같다. 37℃에서 10분, 95℃에서 3분 후 95℃ 35초 - 56℃ 35초 - 72℃ 2분을 34회 반복하고 추가로 72℃에서 10분 반응시켰다.64 μl of DNA polymerase and 50 μl of uracil DNA glucosylase were added to the PCR solution tube, and the mixture was sufficiently mixed, and 30 μl of the PCR mixture was dispensed into the PCR plate. The plate for genotype-specific extension reaction was placed on the magnetic plate for 2 minutes or until the beads were driven to one place, and the supernatant except the beads (about 50 µl) was removed, followed by 50 µl of the general buffer. The beads were placed on a magnetic plate for 2 minutes to remove the supernatant, and 35 μl of DNA dissociation solution was added to each compartment of the genotyping-specific extension plate. The mixture was mixed for 1 minute at 1,800-1,900 rpm to release the beads and placed on a heater preheated to 95 ° C for 1 minute. The plate was removed from the heater and the plate was placed on the magnetic plate for 2 minutes or until the beads gathered and then 30 μl supernatant was transferred to the PCR plate. PCR plates were heat sealed and immediately transferred to gene amplification equipment. The conditions of the PCR reaction are as follows. After 10 minutes at 37 ° C and 3 minutes at 95 ° C, 95 ° C 35 seconds-56 ° C 35 seconds-72 ° C 2 minutes were repeated 34 times and further reacted at 72 ° C for 10 minutes.

비드용액을 충분히 혼합한 후, 20㎕씩 PCR 플레이트로 옮겨 잘 섞은 후 필터 플레이트로 옮겼다. 커버로 덮고 빛을 차단한 채로 상온에서 60분간 두어 결합이 일어나도록 하였다. VeraCode 비드 플레이트 용 중간 플레이트 준비 빈 96 칸 규격의 폐물 수거 플레이트 위에 필터 플레이트 어댑터를 놓고 PCR 반응액이 들어있는 필터 플레이트를 올려놓았다. 25에서 1,000xg로 5분 동안 원심분리하였다. 50㎕의 UB2를 필터 플레이트에 넣고 25℃에서 1,000xg로 5분 동안 원심분리하였다. 30㎕의 혼성용액을 중간 플레이트에 분주하였다. 중간 플레이트와 폐물 수거 플레이트를 교체하고 30㎕의 0.1N 수산화 나트륨을 필터 플레이트에 분주하였다. 즉시 25℃에서 1000xg로 5분간 원심분리하였다. After the bead solution was sufficiently mixed, 20 μl was transferred to the PCR plate, mixed well, and then transferred to the filter plate. Covered and placed for 60 minutes at room temperature while blocking the light to allow the coupling to occur. Intermediate Plate Preparation for VeraCode Bead Plates The filter plate adapter was placed on an empty 96-space waste collection plate and the filter plate containing the PCR reaction solution was placed. Centrifuge for 5 min at 25 × 1,000 × g. 50 μl of UB2 was placed in a filter plate and centrifuged at 1,000 × g for 5 minutes at 25 ° C. 30 μl of hybrid solution was dispensed into the intermediate plate. The intermediate plate and waste collection plate were replaced and 30 μl of 0.1N sodium hydroxide was dispensed into the filter plate. Immediately centrifuged for 5 min at 1000xg at 25 ° C.

15㎖ 튜브에 3㎖의 혼성용액과 3㎖의 0.1N 수산화 나트륨을 넣고 충분히 혼합하여 혼성용액를 중화시켰다. 50㎕의 중화된 혼성용액를 중간 플레이트의 각 칸에 분주하고 중간 플레이트 안의 시료를 4~5회 피펫으로 섞어주었다. 100㎕의 반응체를 VeraCode 비드 플레이트에 옮긴 후 시료가 들어있는 VeraCode 비드 플레이트를 3시간 동안 혼성시켰다(850 rpm, 45℃). VeraCode 비드 플레이트에 200㎕의 세척액을 첨가하고 각 칸의 용액을 피펫으로 섞어주었다. 각 칸 바닥에 비드가 모이도록 2분 동안 기다린 후 상층액을 제거하였다. 반응과정이 끝난 VeraCode 비드 플레이트를 비드익스프레스 리더(BeadXpress reader)로 스캐닝하였다. 상기의 실험은 Illumina사의 GoldenGateGenotyping Kit For Veracode (VC-201-0096, VC-201-0384) 및 BeadXpress reader를 이용한 방법이다.3 ml of the mixed solution and 3 ml of 0.1 N sodium hydroxide were added to a 15 ml tube, and the mixture was mixed well to neutralize the mixed solution. 50 μl of neutralized hybrid solution was dispensed into each compartment of the intermediate plate and the sample in the intermediate plate was mixed with a pipette 4-5 times. 100 μl of the reactant was transferred to a VeraCode bead plate and the VeraCode bead plate containing the sample was hybridized for 3 hours (850 rpm, 45 ° C.). 200 μl of wash solution was added to the VeraCode bead plate and the solution of each compartment was pipetted. Wait 2 minutes for beads to collect at the bottom of each compartment and remove supernatant. After completion of the reaction, VeraCode bead plates were scanned with a BeadXpress reader. The above experiment was performed using Illumina's GoldenGate Geneticping Kit For Veracode (VC-201-0096, VC-201-0384) and BeadXpress reader.

스캐닝이 완료되면 비드스튜디오 소프트웨어를 이용하여 지노타입을 결정하였다. 최종 지노타입은 키트 내의 설명에 따라 비드스튜디오 소프트웨어의 보고서 형식을 이용하여 파일 형태로 출력한다. After scanning was completed, the genotype was determined using the bead studio software. The final genotype is output as a file using the report format of the BeadStudio software as described in the kit.

실시예Example 3:  3: 지노타이핑Zino Typing 분석 결과 및 SNP 유전자 선발 Assay and SNP Gene Selection

실시예 2의 수행 결과 3,072개의 SNP 가운데 2,402개가 성공적으로 지노타이핑되었다. 그 중 MAF(minor allele frequency)가 0.3 이상인 SNP 총 384개를 선발하여 이를 한우 개체인식 및/또는 혈통확인 SNP 유전자 세트로 이용하였다. 하기 표 2는 선발된 384개의 SNP 유전자의 지노타입(Genotype), MAF(minor allel frequency), 이형접합률(Heterozygosity) 및 HWE(Hardy-Weinberg equilibrium)을 나타낸다. 표 2의 MAF 0.3 이상의 SNP는 메이저 대립유전자(major allele) 뿐만 아니라 마이너 대립유전자(minor allele)의 출현빈도가 높아 한우 개체확인을 위한 유전적 다양성이 충분하다.As a result of performing Example 2, 2,402 of 3,072 SNPs were successfully genotyped. Among them, a total of 384 SNPs with a minor allele frequency (MAF) of 0.3 or higher were selected and used as a set of SNP genes for Hanwoo individual identification and / or pedigree. Table 2 below shows the genotype, minor allel frequency (MAF), heterozygosity, and Hardy-Weinberg equilibrium (HWE) of 384 SNP genes selected. SNPs with a MAF of 0.3 or higher in Table 2 have a high frequency of minor alleles as well as major alleles, and sufficient genetic diversity for identification of Hanwoo cattle.

Figure 112007089002752-pat00001
Figure 112007089002752-pat00001

Figure 112007089002752-pat00002
Figure 112007089002752-pat00002

Figure 112007089002752-pat00003
Figure 112007089002752-pat00003

Figure 112007089002752-pat00004
Figure 112007089002752-pat00004

Figure 112007089002752-pat00005
Figure 112007089002752-pat00005

Figure 112007089002752-pat00006
Figure 112007089002752-pat00006

Figure 112007089002752-pat00007
Figure 112007089002752-pat00007

Figure 112007089002752-pat00008
Figure 112007089002752-pat00008

한우 개체인식 SNP 유전자 384개의 MAF 분포는 MAF 0.3~0.35가 30개 (7.8%), 0.35~0.4가 108개 (28.1%), 0.4~0.45가 120개 (31.3), 0.45~0.5가 126개 (32.8%)로 이형접합체(heterozygote) 및 마이너 동형접합체(minor homozygote)의 출현 비율을 높였다. 또한 MAF 0.4 이상의 유전자가 246개로 한우 개체인식에 이용되는 유전자보다 더 큰 유전적 다양성을 필요로 하는 한우 혈통확인에도 이용 가능하였다. 한우개체인식 및/또는 혈통확인용 SNP 유전자 384개의 서열 정보는 표 3에 정리하였다.The MAF distribution of 384 Korean cattle-recognized SNP genes consisted of 30 (7.8%), 0.35 to 0.45, 108 (28.1%), 120 (31.3), 0.4 to 0.45, and 126 (0.45 to 0.5) 32.8%) increased the rate of heterozygote and minor homozygote. In addition, 246 genes with a MAF of 0.4 or more were available for the identification of Hanwoo lineage, which requires greater genetic diversity than the genes used for Hanwoo individual identification. Sequence information of 384 SNP genes for recognition of Korean cattle and / or lineage is summarized in Table 3.

Figure 112007089002752-pat00009
Figure 112007089002752-pat00009

Figure 112007089002752-pat00010
Figure 112007089002752-pat00010

Figure 112007089002752-pat00011
Figure 112007089002752-pat00011

Figure 112007089002752-pat00012
Figure 112007089002752-pat00012

Figure 112007089002752-pat00013
Figure 112007089002752-pat00013

Figure 112007089002752-pat00014
Figure 112007089002752-pat00014

Figure 112007089002752-pat00015
Figure 112007089002752-pat00015

Figure 112007089002752-pat00016
Figure 112007089002752-pat00016

상기 표에서 [A/B]란 염기 A, T, G, C를 예로 든 것으로 염기 A가 B로 점돌연변이된 SNP를 가짐을 의미한다.[A / B] in the above table refers to bases A, T, G, and C, which means that base A has SNPs point-mutated to B.

실시예Example 4: 선발된 SNP 유전자 세트의 검정 (통계분석) 4: Assay of Selected SNP Gene Set (Statistical Analysis)

한우개체인식은 상기 선발된 384개의 SNP 유전자의 각각의 지노타입(AA, AB, BB 형)이 100% 일치하는 경우 동일 개체, 100% 일치하지 않는 경우 독립된 개체로 하였다. Hanwoo individual recognition was the same individual when each genotype (AA, AB, BB type) of the selected 384 SNP genes are 100% identical, independent individuals if 100% does not match.

도 1의 예에서 후대소 HW-X1의 384개 SNP 유전자의 지노타입이 HW003과 100% 일치하여 두 개체가 동일한 한우임을 알 수 있다. HW-X2의 경우도 HW567과 100% 일치하는 지노타입을 보여 동일한 개체로 판단할 수 있다. 지노타입(Genotype) 비교 분석 시 결실이 있는 경우 일치하는 것으로 가정하였다.In the example of FIG. 1, the genotypes of the 384 SNP genes of later major HW-X1 are 100% identical to HW003, indicating that the two individuals are the same Hanwoo. In the case of HW-X2, the genotype that is 100% identical to HW567 can be seen as the same individual. In the genotype comparison analysis, it was assumed that if there were any deletions, they matched.

또한 동일한 384개 유전자 세트는 후대소의 혈통확인에도 이용되었다. 종모우 57 두와 후대소 139 두를 무작위로 섞어 개체 정보를 알 수 없는 조건에서 유전자형을 비교하였다. 종모우의 대립유전자(A, B 형) 중 1개가 후대소와 일치할 경우 그 대립유전자는 종모우로부터 유전된 것으로 보고, 384개 유전자 모두에서 일치할 경우 혈통관계가 성립된 것으로 보았다. 도 2의 예에서 HW-Y1은 HW003과 서로 대립유전자(A, B 형) 중 1개가 서로 일치한다. 이때 실제 혈통확인의 측면에서는 양쪽이 모두 동형접합체(homozygote)인 유전자형을 가진 경우만 비교 가능하며 검정력이 있다고 할 수 있다. The same set of 384 genes was also used to identify lineages in later generations. Randomly mixed 57 cows and 139 cows were compared for genotyping under unknown conditions. If one of the alleles (types A and B) matched the later major cattle, the allele was inherited from the cattle, and if all 384 genes matched, the lineage was established. In the example of FIG. 2, HW-Y1 coincides with HW003 and one of alleles (types A and B). In this regard, in terms of actual lineage, it is possible to compare only when both genotypes are homozygote (homozygote) and have power.

결과:result:

한우 개체인식 및 혈통확인 결과Hanwoo individual identification and pedigree identification

총 384개 SNP 유전자 세트의 실증성 검증을 위해 한우 570 두에서 한우 개체인식 능력을 검사하였다. 한우 별 384개 SNP 유전자의 각 지노타입(AA, AB, BB 형)을 비교하였을 때 384개의 SNP 유전형이 서로 100% 일치하는 경우 동일 개체, 100% 일치하는 개체가 없는 경우 독립된 개체로 하였다. 분석결과 570 두의 한우 가운데 지노타입이 100% 일치하는 경우는 없었다 (1차 검정). 지노타입이 같은 개체가 없음을 확인한 후 2차 검정을 실시하였다. 2차 검정(재검정)은 시료의 정보를 알 수 없는 조건에서 1차 개체인식 SNP 유전자의 결과와 동일한 지노타입을 보이는 개체를 찾아냄으로서 한우 개체인식 SNP 유전자의 검정력을 확인하기 위해 수행하였다. 분석결과, 2차 검정으로 한우 570 두 모두 1차 검정결과와 일치하는 개체를 각각 확인할 수 있었으며 이는 384개의 SNP 유전자 세트가 한우 개체인식 SNP 유전자로서 활용 가능하며 결과의 재현성이 뛰어남을 보인 것이다. Hanwoo individual recognition ability was tested in 570 Korean cattle for the validation of a total of 384 SNP gene sets. When comparing the genotypes (AA, AB, BB) of 384 SNP genes by Korean cattle, 384 SNP genotypes were 100% identical to each other, and the same individuals were found. As a result, there was no 100% coincidence of the genotype among 570 Korean cattle (first-order test). After confirming that no individuals with the same genotype existed, a second assay was performed. A second assay (retest) was performed to identify the power of the Hanwoo trait SNP gene by finding individuals with the same genotype as the results of the primary trait SNP gene under conditions of unknown sample information. As a result of the analysis, it was possible to identify individuals that matched the results of the primary test, respectively, by the secondary test, which shows that 384 SNP gene sets can be used as the SNP genes for the recognition of the Korean cattle and the results are excellent.

한우 개체인식 SNP 유전자로서의 활용성 검정과 동시에 혈통확인 능력도 검정하였다. 본 발명을 위한 한우 570 두 확보 시 종모우 57 두와 후대소 139 두를 포함시켰으며, 후대소 139 두는 무작위로 섞어 개체 정보를 알 수 없는 조건에서 지노타입을 비교하였다. 종모우의 대립유전자(A, B 형) 중 1개가 후대소와 일치할 경우 그 대립유전자는 종모우로부터 유전된 것으로 보고, 384개의 SNP 유전자 모두에서 일치할 경우 혈통관계가 성립된 것으로 보았다. 분석결과, 후대소 139 두 전체에서 384개 SNP 유전자의 대립유전자(A, B 형)가 100% 일치하는 종모우를 찾아낼 수 있었으며 이 결과로 본 발명의 한우 개체인식 SNP 유전자 세트가 한우 혈통확인에도 이용 가능함을 보였다.   As well as the utilization test as a SNP gene for Hanwoo individual identification, the lineage identification ability was also tested. For securing the present invention, 570 cows were included, and 57 cows and 139 cows were included, and 139 cows were randomly mixed to compare genotypes under conditions of unknown individual information. When one of the alleles (types A and B) of the cattle matched the later major, the allele was inherited from the cattle, and if all three of the 384 SNP genes matched, the lineage was established. As a result, it was possible to find 100% matched alleles (types A and B) of 384 SNP genes in all 139 sublarvae. Showed available.

본 발명의 SNP 유전자 세트를 이용하여 최초 송아지의 유전자 검사를 수행한 후 이 자료를 데이터베이스에 등록해두면 추후 한우가 유통되었을 때 동일 유전자 세트로 검사를 하였을 때 데이터베이스에 이미 등록되어 있던 최초 송아지 정보와 일치하므로 한우임을 알 수 있다. 반대로 수입육과 같은 경우 유전자 세트를 이용한 검사결과가 등록되어있지 않으므로 한우로 인정될 수 없다. 이와 같은 원리로 한우/비한우 원산지 판별이 가능하다. When the genetic examination of the first calf is performed using the SNP gene set of the present invention and the data is registered in the database, it is consistent with the first calf information that is already registered in the database when the same cattle are distributed in the future. So you can see that it is Hanwoo. Conversely, in the case of imported meat, the test results using the gene set are not registered, so it cannot be recognized as Korean beef. Based on this principle, it is possible to determine the origin of Korean beef / non-Korean beef.

현재 한우 생산이력체계는 최초 송아지에 부여했던 고유의 이표번호를 한우의 출생에서 소비까지 전산시스템으로 운영하는 컴퓨터정보기술을 기반으로 하는 체계이다. 그러나 이와 같은 생산이력체계는 한우의 고유번호가 오기/유실된 경우나 도축 후 유통과정에서는 추적이 불가능하다. 따라서 최근의 생산이력체계는 소 DNA 마커를 이용한 유전자 감식기법을 도입하는 추세이다. 그러나 현재 주로 이용되는 MS(microsatellite) 마커는 분석조건(기기, 시약, 실험자 등)에 따라 결과가 일정치 않아 사후에 한우개체인식 분석을 할 경우 결과가 부정확할 가능성이 매우 높으며, 검사비용이 고가인 단점이 있다. 이에 본 발명에서는 유전자 지노타이핑 실험의 재현성이 뛰어나고 분석이 용이한 SNP를 이용한 유전자 세트를 개발하였다. Currently, the Hanwoo production history system is a system based on computer information technology that operates the unique identification number assigned to the first calf as a computerized system from birth to consumption. However, such a production history system cannot be traced if the unique number of the Korean cattle is lost or lost or in the distribution process after slaughter. Therefore, the recent production history system is a trend to introduce genetic identification techniques using small DNA markers. However, MS (microsatellite) markers currently used are inconsistent depending on the analysis conditions (instrument, reagents, experimenters, etc.). Therefore, the results may be inaccurate when Hanwoo individual recognition is carried out. There is a disadvantage. Accordingly, the present invention has developed a gene set using SNP that is excellent in reproducibility of gene genotyping experiments and easy to analyze.

본 발명에서 다수의(384개) SNP 유전자를 이용한 것은 유전자의 개수가 증가할수록 정확도는 기하급수적으로 높아지기 때문이며, 최근 분석기술의 발달로 유전자 개수 증가에 따른 분석비용 증가라는 단점을 극복할 수 있는 여러 가지 방법이 개발되었기 때문이다.In the present invention, the use of a large number of (384) SNP genes is because the accuracy is increased exponentially as the number of genes increases, the recent development of analytical technology has been able to overcome the disadvantages of increased analysis cost due to the increase in the number of genes This is because several methods have been developed.

본 발명에서 개발한 SNP 유전자 세트를 이용하여 국내 약 200 만두 한우의 유전형을 각각 데이터베이스화하여 체계화시킨다면 현재의 한우생산이력체계의 단점을 보완하여 고유 이표번호가 없는 모근, 혈액, 고기 상태에서도 한우 개체인식이 가능할 것이며, 정확한 한우 혈통확인을 통하여 정부기관의 한우육종/관리사업에 기반 기술로 이용될 것이다. 또한 전체 한우유전자가 데이터베이스에 등록됨으로서 고의로 오기되어 유통되는 수입육을 선별해 내는 등 쇠고기 원산지 판별이 가능할 것이다.Using the SNP gene set developed in the present invention, if the genotypes of approximately 2 million Korean cattle were databased and organized in Korea, complementing the shortcomings of the current Hanwoo production history system, Hanwoo individual without hairs, blood and meat without a unique target number. It will be able to be recognized and used as a base technology for the Korean beef breeding / management project of government agencies through accurate identification of Hanwoo lineage. In addition, as the entire Hanwoo Gene is registered in the database, it will be possible to determine the origin of beef by screening imported meat that is intentionally imported and distributed.

지금까지 본 발명을 바람직한 실시예를 참조하여 설명하였지만, 본 발명의 상기한 실시예에 한정되는 것은 아니며, 이하의 특허청구범위에서 청구하는 본 발명의 요지를 벗어남이 없이 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자라면 누구든지 다양한 변형 또는 수정이 가능한 범위까지 본 발명의 기술적 사상이 미친다 할 것이다. While the present invention has been described with reference to preferred embodiments, it is not limited to the above-described embodiment of the present invention, and the present invention belongs to the present invention without departing from the gist of the present invention as claimed in the following claims. Anyone with ordinary knowledge will have the technical idea of the present invention to the extent that various changes or modifications can be made.

도 1은 본 발명의 SNP 유전자 세트를 이용한 한우의 개체인식 방법의 예를 나타낸다. A는 한우 종모우 57 두의 384개 SNP 유전자의 지노타입을 나타낸 한우 유전자 데이터베이스이고, B는 후대소의 384개 SNP 유전자의 지노타입을 나타낸 실험군이다. Figure 1 shows an example of the individual recognition method of Hanwoo using the SNP gene set of the present invention. A is a Korean cattle gene database showing the genotypes of 384 SNP genes of 57 cows, and B is an experimental group showing the genotypes of 384 SNP genes of later generations.

도 2는 본 발명의 SNP 유전자 세트를 이용한 한우의 혈통확인 방법의 예를 나타낸다. A는 한우 종모우 57 두의 384개 SNP 유전자의 지노타입을 나타낸 한우 유전자 데이터베이스이고, B는 후대소의 384개 SNP 유전자의 지노타입을 나타낸다. Figure 2 shows an example of a method for identifying lineage of Korean cattle using the SNP gene set of the present invention. A is a Korean cattle gene database showing the genotypes of 384 SNP genes of 57 Korean cattle cattle, B represents the genotypes of 384 SNP genes of later generations.

<110> SNP GENETICS INC. <120> SNP GENES SET FOR IDENTIFYING INDIVIDUAL AND BLOODLINE OF HANWOO <130> IPM-34417 <160> 384 <170> KopatentIn 1.71 <210> 1 <211> 51 <212> DNA <213> Bos taurus <400> 1 ccagggccta gatcgttttc tgcccagggg tggatccctg gccatcacca t 51 <210> 2 <211> 51 <212> DNA <213> Bos taurus <400> 2 tgtctccatc ctcccttcta gttctaatct ttaaacatac tgataagaag g 51 <210> 3 <211> 51 <212> DNA <213> Bos taurus <400> 3 tttgcagatg aggggatatt ttccaagaga gagttgcctt tcttgtctga a 51 <210> 4 <211> 51 <212> DNA <213> Bos taurus <400> 4 tcacattttt actttttagt atttcgtaat gattcaaagc agaactatct t 51 <210> 5 <211> 51 <212> DNA <213> Bos taurus <400> 5 ggaaattcaa aaagataaca cttttgctga gtgtttgaat cttgagttga a 51 <210> 6 <211> 51 <212> DNA <213> Bos taurus <400> 6 acagtgaacc atgacaagta attatcgtga cctttgaaat tttaatatat g 51 <210> 7 <211> 51 <212> DNA <213> Bos taurus <400> 7 gattggaaca atttagggag gcaagcgtga ttttctgcag ttccttcaac a 51 <210> 8 <211> 51 <212> DNA <213> Bos taurus <400> 8 cggaatctcg attaggatgt tcccatgtgt cttattaaaa ggctgccagg c 51 <210> 9 <211> 51 <212> DNA <213> Bos taurus <400> 9 atgtcttcaa tgaactaaac tgaaccaagc tattattact gaattgcaaa c 51 <210> 10 <211> 51 <212> DNA <213> Bos taurus <400> 10 tttttaggga aaccatacat tttctaactt ttgaaaaatg caagcactca g 51 <210> 11 <211> 51 <212> DNA <213> Bos taurus <400> 11 atttctcatt gtgcttgttt attcagcttg aacacttctg tgcattgggt a 51 <210> 12 <211> 51 <212> DNA <213> Bos taurus <400> 12 gatgatattc aggctttagc taagacttat tgtaaggcaa ccatgatctg t 51 <210> 13 <211> 51 <212> DNA <213> Bos taurus <400> 13 atgcacaacg tttctagaga cgccagtgtg aaggcaggtg ctttgcaacc t 51 <210> 14 <211> 51 <212> DNA <213> Bos taurus <400> 14 actaggcttg aatgcagttg ctgtggaatg cactaattaa ttacatttat t 51 <210> 15 <211> 51 <212> DNA <213> Bos taurus <400> 15 gacttatcac tctatcaagt ttatttatat ttgattcatc ccttacaact g 51 <210> 16 <211> 51 <212> DNA <213> Bos taurus <400> 16 atcatcagtt atcatctctg atttgatttt aatagaaaca atggagcctg t 51 <210> 17 <211> 51 <212> DNA <213> Bos taurus <400> 17 tgtgtattcc tatttaaaaa tgcctgccac gttatatgac ctcatgttaa t 51 <210> 18 <211> 51 <212> DNA <213> Bos taurus <400> 18 ttctgggtct tgaggatgtg aaaccgtcta ctgaaaccat atttgtaaat g 51 <210> 19 <211> 51 <212> DNA <213> Bos taurus <400> 19 tcgtgcaata gaaatacaag caatcttgta tattttggca gatagtgccc g 51 <210> 20 <211> 51 <212> DNA <213> Bos taurus <400> 20 cttgaggtga tttagatggg tgtctgaggc tatctttgga tgtacttttg t 51 <210> 21 <211> 51 <212> DNA <213> Bos taurus <400> 21 agtagggtcc aagaagcaga gagtagaagt gcaaaacctc aaaaatgatg a 51 <210> 22 <211> 51 <212> DNA <213> Bos taurus <400> 22 ctcttgagga atttgctgat gactttaaaa aaaaagcacc aaacactgta a 51 <210> 23 <211> 51 <212> DNA <213> Bos taurus <400> 23 caagtttaac acaaaagaaa attttaaaag cctgagtttt taaagtaggt g 51 <210> 24 <211> 51 <212> DNA <213> Bos taurus <400> 24 agtgggaata atgagaagca gttgcgttgt tttctatcat ttttaggttt t 51 <210> 25 <211> 51 <212> DNA <213> Bos taurus <400> 25 tgttctttcc aaagactctg tgggggtaca ttttcaatgt ggagagcaat a 51 <210> 26 <211> 51 <212> DNA <213> Bos taurus <400> 26 aggcagatga tatttcaagt taaacgtcaa gtatattgtc cttaccatca g 51 <210> 27 <211> 51 <212> DNA <213> Bos taurus <400> 27 tgactaaatt aaccccaccc cacccgcaat tctcttttta tcagtacctc t 51 <210> 28 <211> 51 <212> DNA <213> Bos taurus <400> 28 caaggcccag aaagaggcaa actggtactt ctggaccacc ataccagctg g 51 <210> 29 <211> 51 <212> DNA <213> Bos taurus <400> 29 aaacccacaa tatcacacac tcccatgtcc ttattccctc tgaaaagtct c 51 <210> 30 <211> 51 <212> DNA <213> Bos taurus <400> 30 tataattctg tgaaactcga gcttgacatt aatttttact ttttacaaag c 51 <210> 31 <211> 51 <212> DNA <213> Bos taurus <400> 31 cctcagcctg ctgctcacgg catgcgttcg ctatattctg cgttatttat t 51 <210> 32 <211> 51 <212> DNA <213> Bos taurus <400> 32 atgttgttag atgagtactc tctctttttt tttttttggc tatgtatcta g 51 <210> 33 <211> 51 <212> DNA <213> Bos taurus <400> 33 cggctcatga aggaatagtt tcttggcttc tcgagcccct tgttttggat g 51 <210> 34 <211> 51 <212> DNA <213> Bos taurus <400> 34 tagtatgtca aacatgaagg ttaaatcaac ctcgtgaatt aaaaaaattc c 51 <210> 35 <211> 51 <212> DNA <213> Bos taurus <400> 35 ctgagaagca ccctggcaca aggctggcct tctgcagctg atggccacct t 51 <210> 36 <211> 51 <212> DNA <213> Bos taurus <400> 36 agagactaga aaaaggcagg tctgtcgaat ctaaaacatt tggcaaactg a 51 <210> 37 <211> 51 <212> DNA <213> Bos taurus <400> 37 tgttaggagt aggagatgaa gtggacatac acaccattca ttatcatgct g 51 <210> 38 <211> 51 <212> DNA <213> Bos taurus <400> 38 tttttgtgct ttaaaaagaa aaaaaactct ctgagcttca aatgagagtt t 51 <210> 39 <211> 51 <212> DNA <213> Bos taurus <400> 39 tcaccagagg gcagcgctgt gccattgccc tctggttcac tttggatgct c 51 <210> 40 <211> 51 <212> DNA <213> Bos taurus <400> 40 ttattttcta gagtgttggt ggggttatgt tgatctactt acttcttttc c 51 <210> 41 <211> 51 <212> DNA <213> Bos taurus <400> 41 gtgtggtcag atgcagtgtg ccttgtgggt ataaaattaa tttagcaccg a 51 <210> 42 <211> 51 <212> DNA <213> Bos taurus <400> 42 gctactcttg ttttaagtaa attctgtgtc acatgtattc cttactgtta t 51 <210> 43 <211> 51 <212> DNA <213> Bos taurus <400> 43 gtacaacatc ataaataatg aaatactgta atgaaattgt atgggaacct a 51 <210> 44 <211> 51 <212> DNA <213> Bos taurus <400> 44 agcttcagtg cagaattaac agagctccct tgcttaggtt taatttggat a 51 <210> 45 <211> 51 <212> DNA <213> Bos taurus <400> 45 taacacctag actcatactt tggcctcagc agtacagagg atacaggtat t 51 <210> 46 <211> 51 <212> DNA <213> Bos taurus <400> 46 caggttgtgc tctgcaaatc acaaacgtta ggaagagcaa taattgcagt a 51 <210> 47 <211> 51 <212> DNA <213> Bos taurus <400> 47 tttgtagagt aaatactctt aaaccatttg tatgtagaca aacatctaac t 51 <210> 48 <211> 51 <212> DNA <213> Bos taurus <400> 48 tgctctgctt gttttgctag tcatcgtttg gttgattgaa taatgatgat g 51 <210> 49 <211> 51 <212> DNA <213> Bos taurus <400> 49 gaaccggctc cgcgtatatt tagttgtaat tcctaaactg aaaagtggct a 51 <210> 50 <211> 51 <212> DNA <213> Bos taurus <400> 50 ttaaaaaatg atcataaggg aaaatggaat gaatagttgg tttgggggta a 51 <210> 51 <211> 51 <212> DNA <213> Bos taurus <400> 51 tttgcttcat gaggagatta cactgaggta atacattctg aacgttgaaa c 51 <210> 52 <211> 51 <212> DNA <213> Bos taurus <400> 52 atttcacaga gaccacaaaa cccgcgtcag gtgtgaaagt gctttacctg a 51 <210> 53 <211> 51 <212> DNA <213> Bos taurus <400> 53 tcactaggat tcagtgaagc ctctggaact ccggctccgc gtggacaggg t 51 <210> 54 <211> 51 <212> DNA <213> Bos taurus <400> 54 tgtagttttt aaaatatcat aaagacgagt ctcacaaaag ggaatctttc a 51 <210> 55 <211> 51 <212> DNA <213> Bos taurus <400> 55 agctgatgag cagtgaaata aatctattca gtcaacggag attgggttta t 51 <210> 56 <211> 51 <212> DNA <213> Bos taurus <400> 56 agaaacagca tatcaaattt cttctcattt ctatctgtct cccagtgaaa t 51 <210> 57 <211> 51 <212> DNA <213> Bos taurus <400> 57 gccattcagg tctcctcctg agctccgtct ccagtgcatc acaagagtcc t 51 <210> 58 <211> 51 <212> DNA <213> Bos taurus <400> 58 tagtggtacc ctcactcctg ggctcgaaga gtcataataa tccccttcct t 51 <210> 59 <211> 51 <212> DNA <213> Bos taurus <400> 59 atatgatgac aaacaagaaa acaattataa agaaaaacta tagccaggag g 51 <210> 60 <211> 51 <212> DNA <213> Bos taurus <400> 60 ggtcccctcg ccaggccacc ccaacgctga gctatttggg gaattgcttc c 51 <210> 61 <211> 51 <212> DNA <213> Bos taurus <400> 61 ataagggcag agacctattt aattcttctc aacagtgcct agagttaagt a 51 <210> 62 <211> 51 <212> DNA <213> Bos taurus <400> 62 ctcttattta ctctcagagt atccatgaag ctgaagcttg tgatgggttt t 51 <210> 63 <211> 51 <212> DNA <213> Bos taurus <400> 63 catataaaat catcttgaga aagacgatgg atgggaaaga agacatcttt t 51 <210> 64 <211> 51 <212> DNA <213> Bos taurus <400> 64 gcttgtcttt aaaaaatgta tacagttaaa gcataataaa aatgccccaa t 51 <210> 65 <211> 51 <212> DNA <213> Bos taurus <400> 65 cagacataaa tactgctctc caggatcctc tgttcctttc agagttttga t 51 <210> 66 <211> 51 <212> DNA <213> Bos taurus <400> 66 taattgattc tactaactaa aacttgtcct cacatttggg attaaacttg a 51 <210> 67 <211> 51 <212> DNA <213> Bos taurus <400> 67 cttgcattgt tcttctcaaa aatgttggct cattcagtca cttctatcag g 51 <210> 68 <211> 51 <212> DNA <213> Bos taurus <400> 68 gcatttatta agtctctgag cccagcgccc tgaagtcaaa attctagaaa c 51 <210> 69 <211> 51 <212> DNA <213> Bos taurus <400> 69 ttctggaagc tgtactcttg ggcttggacc atgtgttgag acaaaacaga c 51 <210> 70 <211> 51 <212> DNA <213> Bos taurus <400> 70 taagcttgtg atatattgca gattatagaa tcctgtacct tcatagatct a 51 <210> 71 <211> 51 <212> DNA <213> Bos taurus <400> 71 taacagatta atactgtttc tgtcttcttc aattatacac tgtgcactta g 51 <210> 72 <211> 51 <212> DNA <213> Bos taurus <400> 72 cgtatggaaa cctggagaaa ggcccgggaa cacacagggc cagaggcatc c 51 <210> 73 <211> 51 <212> DNA <213> Bos taurus <400> 73 ttgcagctgt tttttatttg atcttttaaa gatccaaaga tgttcaagga t 51 <210> 74 <211> 51 <212> DNA <213> Bos taurus <400> 74 cagccgtaag actctccagc acatctatca gcttggcatt gctctctcat g 51 <210> 75 <211> 51 <212> DNA <213> Bos taurus <400> 75 ccacttttcc ctcgcacgct tcctcggtag cagccctatt tgcggatctt c 51 <210> 76 <211> 51 <212> DNA <213> Bos taurus <400> 76 acaggacgct tccctgagtg tagaccgagc tctacacagc tcttcagaag t 51 <210> 77 <211> 51 <212> DNA <213> Bos taurus <400> 77 actcgctccg gatcctagac tgcagcttca accgtatcat ggcctctaag g 51 <210> 78 <211> 51 <212> DNA <213> Bos taurus <400> 78 gaaatcaaga tatgaaatag caatatagga aacacaaaaa agctgagttt g 51 <210> 79 <211> 51 <212> DNA <213> Bos taurus <400> 79 tggccagccc gtgccgcgaa cacaccgagt aggggcagat ctcggctccc a 51 <210> 80 <211> 51 <212> DNA <213> Bos taurus <400> 80 gcaggggctg tgctctctgg gggacgtata gaagctgtct ttaccttcaa t 51 <210> 81 <211> 51 <212> DNA <213> Bos taurus <400> 81 tccttaaata taggagctta gtgcaagggt ttatttaaaa ctcacagtat a 51 <210> 82 <211> 51 <212> DNA <213> Bos taurus <400> 82 ttaattttgt atagcttaag cgcccatctc tttaatctat taaaatagag t 51 <210> 83 <211> 51 <212> DNA <213> Bos taurus <400> 83 tttctgttgc tggaaaggag gggcatgagg aactgaagca acagctctag g 51 <210> 84 <211> 51 <212> DNA <213> Bos taurus <400> 84 ttaaagacat tttccttaaa gactttctta taggggagtc catggcaaaa t 51 <210> 85 <211> 51 <212> DNA <213> Bos taurus <400> 85 aatatcaaaa gtatttttcc tgaccgttgt acaatgacac gagttcctca a 51 <210> 86 <211> 51 <212> DNA <213> Bos taurus <400> 86 ttgatgctac gttgaccgga ggccctgcca ggtgagaaga gggatgagca a 51 <210> 87 <211> 51 <212> DNA <213> Bos taurus <400> 87 tttcagtatc tgaacatgct atcaccgtgt aaaacagatt tctttattac a 51 <210> 88 <211> 51 <212> DNA <213> Bos taurus <400> 88 aaattctagt gctccatggc ttagattaac taccctagaa aagttctcca c 51 <210> 89 <211> 51 <212> DNA <213> Bos taurus <400> 89 agctttatta atgccataaa gctattgaaa gaaacgtggc ttacccttgt c 51 <210> 90 <211> 51 <212> DNA <213> Bos taurus <400> 90 cttcactttc ttcagaccaa ttctggttaa aagaaggaaa tacaccttaa c 51 <210> 91 <211> 51 <212> DNA <213> Bos taurus <400> 91 tctgcaggaa ggttatggga gcaccgtgtc agggagacgg cccagctcac a 51 <210> 92 <211> 51 <212> DNA <213> Bos taurus <400> 92 attttgtaaa atttccagct cagaagtccc tcagtgtttt gattattctc c 51 <210> 93 <211> 51 <212> DNA <213> Bos taurus <400> 93 atcatgatac aagtcactgt gttttgttgg aaatgcaaca aataaaattg c 51 <210> 94 <211> 51 <212> DNA <213> Bos taurus <400> 94 cttggtcctc cattgaggtg gacagttcct gcaccctggg gctgaatgag g 51 <210> 95 <211> 51 <212> DNA <213> Bos taurus <400> 95 acaaactcat tggcatcacc catcagtctt actcattatg gctcacagct g 51 <210> 96 <211> 51 <212> DNA <213> Bos taurus <400> 96 tatttttttc atattttaaa aacaatcaag cggacagact tgaaataata a 51 <210> 97 <211> 51 <212> DNA <213> Bos taurus <400> 97 cttaaaatat tgtgtaggac tcaccgctct tagctgttag aatgttgtta a 51 <210> 98 <211> 51 <212> DNA <213> Bos taurus <400> 98 agtagacggt cctgggtgtt gagtcgtatg gatgctagat tgcaataccc t 51 <210> 99 <211> 51 <212> DNA <213> Bos taurus <400> 99 attgtttaag tgcatattag aaagttaaaa atcatatatg tggctaccat t 51 <210> 100 <211> 51 <212> DNA <213> Bos taurus <400> 100 ataaattgaa tagtgtgtat atgtgtgttg gagcttgtag tagcctctga c 51 <210> 101 <211> 51 <212> DNA <213> Bos taurus <400> 101 ctgggccctg ggatatagct ttcccgttgg tgctgattct gtggtccttg c 51 <210> 102 <211> 51 <212> DNA <213> Bos taurus <400> 102 tagaagggcc tgctttacaa agtaccggct gataatgttt tccaatcaac a 51 <210> 103 <211> 51 <212> DNA <213> Bos taurus <400> 103 aaggcaagag gagggcttag attaggaaaa gtacaaacag aaaagtcaac t 51 <210> 104 <211> 51 <212> DNA <213> Bos taurus <400> 104 aaatatttcc ccccagaaag caagagggag agtctgttct tgtggagatc t 51 <210> 105 <211> 51 <212> DNA <213> Bos taurus <400> 105 cctccgtgta atacatcaag ttcaatgtcc accaatgaac caaactgcat g 51 <210> 106 <211> 51 <212> DNA <213> Bos taurus <400> 106 gatttgttgc ctttcagacc tggacggctg aatggccaac cttattccta a 51 <210> 107 <211> 51 <212> DNA <213> Bos taurus <400> 107 tcgccctttg gtctaacaat gccagtcttg acttgttgct gtttactaat t 51 <210> 108 <211> 51 <212> DNA <213> Bos taurus <400> 108 ctgccacggg attaaggttc atgccgtaga attaaaggct ttaaaatagc a 51 <210> 109 <211> 51 <212> DNA <213> Bos taurus <400> 109 tcagagcgtt gactaatagg gttaactgat agaagtccag tagtctggtt t 51 <210> 110 <211> 51 <212> DNA <213> Bos taurus <400> 110 aaagcaggag agaaatgtga acctgagaag gtgtttttcg cttgatttag a 51 <210> 111 <211> 51 <212> DNA <213> Bos taurus <400> 111 tgcgtctaaa aatacccgcc ttgggaaatg taaaggtttc ccctgactcc t 51 <210> 112 <211> 51 <212> DNA <213> Bos taurus <400> 112 aagacccaga ctagatgcta catctactaa ggagtcatgt ctaatgtgta c 51 <210> 113 <211> 51 <212> DNA <213> Bos taurus <400> 113 agtaaggcac tggataagtg gggttcgtct gtatagtgga aaactaagca g 51 <210> 114 <211> 51 <212> DNA <213> Bos taurus <400> 114 tcttcttgta ttaatttctc tctttttcca ctcctccaaa accttcaggt a 51 <210> 115 <211> 51 <212> DNA <213> Bos taurus <400> 115 cccacacaaa gggagttctt aatctattac tattacgctg gtgacaatga a 51 <210> 116 <211> 51 <212> DNA <213> Bos taurus <400> 116 gggggttcgt tatgtgaggc aatgcgttgt gtgctctaat cttttctggt a 51 <210> 117 <211> 51 <212> DNA <213> Bos taurus <400> 117 gaaaatccat tgagtgtcat gcagtggtgg gaatggaaat aatcttcttt g 51 <210> 118 <211> 51 <212> DNA <213> Bos taurus <400> 118 gaatataaaa ttgctcgcca tcctctacga atattccttt catacgggta a 51 <210> 119 <211> 51 <212> DNA <213> Bos taurus <400> 119 ggcagtcgag agcctggggc accagtgacg tccaggtcct ctttacccac c 51 <210> 120 <211> 51 <212> DNA <213> Bos taurus <400> 120 tgtgtgtttc atgtctcact ttaacgtgta cctcatcctc atcatgtgca a 51 <210> 121 <211> 51 <212> DNA <213> Bos taurus <400> 121 tcagatgaca atgagaagtg gtacttggtg aggagagtca atgtgacaag g 51 <210> 122 <211> 51 <212> DNA <213> Bos taurus <400> 122 tgtctacagc agaggcaaag acaagtgggg gagaagaatg tttctcaggc a 51 <210> 123 <211> 51 <212> DNA <213> Bos taurus <400> 123 tagaagatac atcagtcatg gatacggtgt tgaggaggaa gaaagcagaa a 51 <210> 124 <211> 51 <212> DNA <213> Bos taurus <400> 124 cttctctgaa gcctctctgt accctaaaca gttgactgtg ggtggagagg t 51 <210> 125 <211> 51 <212> DNA <213> Bos taurus <400> 125 gaaagagata gatgctattt ttacccacat agagcttact gtcagtggtg a 51 <210> 126 <211> 51 <212> DNA <213> Bos taurus <400> 126 tggtgctggc catagttcac ttttatagca atccattaag acagacatta t 51 <210> 127 <211> 51 <212> DNA <213> Bos taurus <400> 127 aactcaatca ggtacaaggc catgcggact tcattctcta aaggatcttt c 51 <210> 128 <211> 51 <212> DNA <213> Bos taurus <400> 128 gattaaaaaa gaatagagaa agtcacgagt gtgcactgat tgcaaaaaag a 51 <210> 129 <211> 51 <212> DNA <213> Bos taurus <400> 129 cagatttttc ttttaactgc tatccgtgtg gcattacatt gtggatattc c 51 <210> 130 <211> 51 <212> DNA <213> Bos taurus <400> 130 agagaaaggt tgggtgagta ggaatattta taacttggcg acaggggaga t 51 <210> 131 <211> 51 <212> DNA <213> Bos taurus <400> 131 acacaaatga acgactactt tacttcactt taatatgacc atcagttaag a 51 <210> 132 <211> 51 <212> DNA <213> Bos taurus <400> 132 tgacactgaa accgcagtat ttgtggattc tgctttcaac tagaatagtc c 51 <210> 133 <211> 51 <212> DNA <213> Bos taurus <400> 133 tctcttttct ttagcataat cgctactgct atattttcag gttcaatttt a 51 <210> 134 <211> 51 <212> DNA <213> Bos taurus <400> 134 ctccctcctt tctctttgta gtggaggaag tggaattcat aattcatttg a 51 <210> 135 <211> 51 <212> DNA <213> Bos taurus <400> 135 accactgagt ctgagctgcc acacaatttt gtcatttcct ttatcatgtc c 51 <210> 136 <211> 51 <212> DNA <213> Bos taurus <400> 136 atgttttatg tttaatgtta ttgctcataa ccgatttttt tagggaacat t 51 <210> 137 <211> 51 <212> DNA <213> Bos taurus <400> 137 cacaccatga cccattatag ctcatttgac aaagagaata aagatgcctg c 51 <210> 138 <211> 51 <212> DNA <213> Bos taurus <400> 138 gtacagtatt atttcagcaa catcaattct tacaggcttc tttgaaaatt t 51 <210> 139 <211> 51 <212> DNA <213> Bos taurus <400> 139 cattttagta tcaattttta aagcactatg cgtcatcgca atttaccata t 51 <210> 140 <211> 51 <212> DNA <213> Bos taurus <400> 140 caaaatcccc atcgggaggc actgatggaa cagcaactag gaaggcacac a 51 <210> 141 <211> 51 <212> DNA <213> Bos taurus <400> 141 caactttgtt ggtggtatat tttacatatc ctgagattta tttattgcag g 51 <210> 142 <211> 51 <212> DNA <213> Bos taurus <400> 142 atctcaataa agactcagaa aaacaccctg aggttgcaac actgtaaatt t 51 <210> 143 <211> 51 <212> DNA <213> Bos taurus <400> 143 taagttgccc agagaaaaag ggacattgta gtccggtttt ctaatctttg t 51 <210> 144 <211> 51 <212> DNA <213> Bos taurus <400> 144 tgctttctct agaatggggt gtgaatcagt agaatttgtg ttgtttctca g 51 <210> 145 <211> 51 <212> DNA <213> Bos taurus <400> 145 atacactctc ttctccagcc tactcggcta cacctcatgt ctgactccgc t 51 <210> 146 <211> 51 <212> DNA <213> Bos taurus <400> 146 acaggcatag ctggttcctc ctgagtacca tgagctgccc caggccccct c 51 <210> 147 <211> 51 <212> DNA <213> Bos taurus <400> 147 ttaggacttc ggttatcaaa atccagtcaa aattccactt taattttgat c 51 <210> 148 <211> 51 <212> DNA <213> Bos taurus <400> 148 tcactatcat attttatttc ctcaggtaaa aattacaatt gataaagaag a 51 <210> 149 <211> 51 <212> DNA <213> Bos taurus <400> 149 tcctcattaa atataggctc tgtgagtata agcatgtttg tctgcttctt t 51 <210> 150 <211> 51 <212> DNA <213> Bos taurus <400> 150 actgagatgc acagtactgg gccccgccct aggtcttctg aattagaatt t 51 <210> 151 <211> 51 <212> DNA <213> Bos taurus <400> 151 caatataact gtccttgaaa aacaatgata aatggacata attgtcaaag a 51 <210> 152 <211> 51 <212> DNA <213> Bos taurus <400> 152 aatgcccaag cacatgaaag agcctggcat caggacttca aagaaatctg t 51 <210> 153 <211> 51 <212> DNA <213> Bos taurus <400> 153 tttattcagc tcactaggat attttcctaa attgacccaa tgaggacata g 51 <210> 154 <211> 51 <212> DNA <213> Bos taurus <400> 154 tttgacataa ttactgtgga tttcctgaac cactcttatg ttatatgcct a 51 <210> 155 <211> 51 <212> DNA <213> Bos taurus <400> 155 tatgaccatg ctgggcatca aggctgaccc agaaagcagt ctgagagcag c 51 <210> 156 <211> 51 <212> DNA <213> Bos taurus <400> 156 tgtacagtta gtgaagtttg gtatagttca gcttttcttt agatcttcca t 51 <210> 157 <211> 51 <212> DNA <213> Bos taurus <400> 157 tctaagaaat agtacagagg gatcctgtga accctttacc cagttttctc a 51 <210> 158 <211> 51 <212> DNA <213> Bos taurus <400> 158 tgttttcagc ttcaagtact ggtgtcttac acgtacccat taactaaaca t 51 <210> 159 <211> 51 <212> DNA <213> Bos taurus <400> 159 agcatgtctg tgttgaactt cagtagtgat gaatatctag aaagcatttt c 51 <210> 160 <211> 51 <212> DNA <213> Bos taurus <400> 160 gatacagatt ctcaggtcct gacacaggag tttggtcctg acactgagtt g 51 <210> 161 <211> 51 <212> DNA <213> Bos taurus <400> 161 tgaggaaaca gaagtagaaa ctagcgttat tttataaatg atcccaaaac c 51 <210> 162 <211> 51 <212> DNA <213> Bos taurus <400> 162 cttgttatct tatatgctgg gcttcgatag aattttccct tacccaggta g 51 <210> 163 <211> 51 <212> DNA <213> Bos taurus <400> 163 taattaaaaa ccataagtta aaacctctgt ctccagaaac ttaaaaccat t 51 <210> 164 <211> 51 <212> DNA <213> Bos taurus <400> 164 tggctttgcc aaggagggga gctccagaaa ccagtgtgat ggtcactgag t 51 <210> 165 <211> 51 <212> DNA <213> Bos taurus <400> 165 aacataactc agaccactgg ctaaaacact ggtagtctta gaacaatgta a 51 <210> 166 <211> 51 <212> DNA <213> Bos taurus <400> 166 ttctcctgac tcaggcacca atatctagac tttgtctttt gttggcccag a 51 <210> 167 <211> 51 <212> DNA <213> Bos taurus <400> 167 gttgggctaa gattatatca agatttaggt ccaggactgt gaattcaagg c 51 <210> 168 <211> 51 <212> DNA <213> Bos taurus <400> 168 agagaggctg agtagagaac tgaggcctat gatatatgac tgtaatcatt c 51 <210> 169 <211> 51 <212> DNA <213> Bos taurus <400> 169 aaaatggtgg ctcttttatg ccactaagtt tttggaaggt ttgttgtgca g 51 <210> 170 <211> 51 <212> DNA <213> Bos taurus <400> 170 gatacattag tagagatcgc atggccgcag attttaaaca cattggtaga t 51 <210> 171 <211> 51 <212> DNA <213> Bos taurus <400> 171 ttgggatttt atatagtgcc catggctgac tttagaaatt aggggaaata a 51 <210> 172 <211> 51 <212> DNA <213> Bos taurus <400> 172 gtagagtaaa agaattttgg agatgtttaa actaaacacc ttgtgagaga g 51 <210> 173 <211> 51 <212> DNA <213> Bos taurus <400> 173 tagaatttat gccattgctt aacatgagaa ttttttttgg tcagctttaa a 51 <210> 174 <211> 51 <212> DNA <213> Bos taurus <400> 174 ggaaagcacc tgtcaataag gaccacgcaa ctgtattttt tgccctgcat a 51 <210> 175 <211> 51 <212> DNA <213> Bos taurus <400> 175 ggaaaagtgc ttaattaaca ctgactagtt ttaacctcct caagtaaggc c 51 <210> 176 <211> 51 <212> DNA <213> Bos taurus <400> 176 ttccttggaa agaaatgagt catctcggta aaaaatatac attaaagcat g 51 <210> 177 <211> 51 <212> DNA <213> Bos taurus <400> 177 gaggtcagtt cctccaaggg actctcggac cttttatttc aggcttgttg t 51 <210> 178 <211> 51 <212> DNA <213> Bos taurus <400> 178 acagggatgt cttgctccct cattgcacag ccccaagctc ctcgaggccc t 51 <210> 179 <211> 51 <212> DNA <213> Bos taurus <400> 179 ctagtgtgtt tgattaaaaa gtgcctcatg agatcctcct aaaatccaaa a 51 <210> 180 <211> 51 <212> DNA <213> Bos taurus <400> 180 cgggacctgg tcacagctcc gtcgccgagt gtctgggtga tgacgtgtcc c 51 <210> 181 <211> 51 <212> DNA <213> Bos taurus <400> 181 gggaaaaaac cagttcagct gaacgtgccc tgctagctct ctgtggaggg c 51 <210> 182 <211> 51 <212> DNA <213> Bos taurus <400> 182 gggctagaac ccgacaagag aagcctccgc aatgagaagc ccacgcatgt a 51 <210> 183 <211> 51 <212> DNA <213> Bos taurus <400> 183 cccagaacag tggagatact tagtactcct gatgcctgta ggcaaagaaa a 51 <210> 184 <211> 51 <212> DNA <213> Bos taurus <400> 184 ttcccttttg cagtattttg gttttgaatt tctcttctta gaagtttggg t 51 <210> 185 <211> 51 <212> DNA <213> Bos taurus <400> 185 gatttgtgct gggcaagaag taaccgtctc tggcaagacc tggcatctgg g 51 <210> 186 <211> 51 <212> DNA <213> Bos taurus <400> 186 ggcctcacac agagaggggc ttggaacctt tgcactgcac ccaggaagac a 51 <210> 187 <211> 51 <212> DNA <213> Bos taurus <400> 187 aagggaaagt ttatgagaaa agacatagct gtgaggaagc agtcagatcc c 51 <210> 188 <211> 51 <212> DNA <213> Bos taurus <400> 188 aattaattca cattctgagg ttctgagcat taggacttct tcatgccccc t 51 <210> 189 <211> 51 <212> DNA <213> Bos taurus <400> 189 tggagttcag tgcccctaaa attataggga aattgccata tgtgatgtcg g 51 <210> 190 <211> 51 <212> DNA <213> Bos taurus <400> 190 gttcctagtg attccaacac actttatttt cttaattaca tcattttagt a 51 <210> 191 <211> 51 <212> DNA <213> Bos taurus <400> 191 ggttgatcag acttgggtca catgtatacc cttagaataa atttcaccag a 51 <210> 192 <211> 51 <212> DNA <213> Bos taurus <400> 192 tataggatat acctaggaat ggaactgata gatcacagga tccatgatga g 51 <210> 193 <211> 51 <212> DNA <213> Bos taurus <400> 193 taattttaaa catgttcaaa agtggggcga atggtgtaac atattctcat g 51 <210> 194 <211> 51 <212> DNA <213> Bos taurus <400> 194 gagcctccaa ctcatttatt tatctatctt cccaacagaa aaagcagcgt c 51 <210> 195 <211> 51 <212> DNA <213> Bos taurus <400> 195 gtatgtaatt gtatacagtg ctaaagtctc attttagggc gagggaaaaa t 51 <210> 196 <211> 51 <212> DNA <213> Bos taurus <400> 196 cttccaaatt tacctccatg tccattgcag gaagaaattg taaggacagt g 51 <210> 197 <211> 51 <212> DNA <213> Bos taurus <400> 197 taacacaaaa aatatgcaac cttccattta caatcaaatg ctaggtgtca t 51 <210> 198 <211> 51 <212> DNA <213> Bos taurus <400> 198 atatttgttt ctgatggttt atcttcacca gaggcatctc aaaccatcta c 51 <210> 199 <211> 51 <212> DNA <213> Bos taurus <400> 199 gggtacttct gagagcatgt gggagtgggg tctgtcctga aaaggcctca c 51 <210> 200 <211> 51 <212> DNA <213> Bos taurus <400> 200 cgacacttcc tgctccctca tctgcatgcc caagctgtca gcacgtctgc c 51 <210> 201 <211> 51 <212> DNA <213> Bos taurus <400> 201 tgtttccaac acctcttttc ctccccaaac cactgttcca aggcacatat t 51 <210> 202 <211> 51 <212> DNA <213> Bos taurus <400> 202 gaaatacaga aatgagctgc atagtgtcct gatgaattat atgcattcca g 51 <210> 203 <211> 51 <212> DNA <213> Bos taurus <400> 203 tttaatgatg aactcaatgt tactagagac agagagctgt tcaaagtttt t 51 <210> 204 <211> 51 <212> DNA <213> Bos taurus <400> 204 ttggaaagtc aaagaaataa actgagtact ttgggctgat ggaagtatgt t 51 <210> 205 <211> 51 <212> DNA <213> Bos taurus <400> 205 tctagacact gcaaagctac acttgagagt gtgtagtaca gtcactattt a 51 <210> 206 <211> 51 <212> DNA <213> Bos taurus <400> 206 ctgtatggag aaaatcctaa agacacggaa aactactgga gcttgttaat t 51 <210> 207 <211> 51 <212> DNA <213> Bos taurus <400> 207 tatcagtcac aacagcagaa aggaaacaca gtgaagtaca tatcatctct t 51 <210> 208 <211> 51 <212> DNA <213> Bos taurus <400> 208 cctgttatct ctgacttagt aacacgttgc tcagagcttg tcacgagatt t 51 <210> 209 <211> 51 <212> DNA <213> Bos taurus <400> 209 ctctctttgt ctctctggct ttccctctca ttagtctgtt taacgagctg g 51 <210> 210 <211> 51 <212> DNA <213> Bos taurus <400> 210 cccatttcct gcagtttcct ctcccaccgt ctagctgctc cgagagccgg t 51 <210> 211 <211> 51 <212> DNA <213> Bos taurus <400> 211 cccatggaag agcttgactt cctctctccc ccagccttga ccctgagcca c 51 <210> 212 <211> 51 <212> DNA <213> Bos taurus <400> 212 gtcccagttg gtggcacatg ggcagctgtt attttcaaga agctttctta g 51 <210> 213 <211> 51 <212> DNA <213> Bos taurus <400> 213 cattctgctt ggtggacaca ttgatgtact aagcagatct caggccctga g 51 <210> 214 <211> 51 <212> DNA <213> Bos taurus <400> 214 gcaagacatg ccgcctcaat gagcctcatt tcatggggac aatcaaaata a 51 <210> 215 <211> 51 <212> DNA <213> Bos taurus <400> 215 agagcccagt atcagataaa tacttttagg gtgaagcaat gaatgatcta a 51 <210> 216 <211> 51 <212> DNA <213> Bos taurus <400> 216 gaatatctta atgaaatcaa gcctccttaa gtgacctata aattcaatat g 51 <210> 217 <211> 51 <212> DNA <213> Bos taurus <400> 217 ttgacatata ccttctttca ggaactgaat catgaagagt ttaatcaagt a 51 <210> 218 <211> 51 <212> DNA <213> Bos taurus <400> 218 gcttgtaatg tagaaatctc cccccagtat aagaaggcta ttcagaagct g 51 <210> 219 <211> 51 <212> DNA <213> Bos taurus <400> 219 tcttttttta cttatatgac atgatagatg ttcactaatt gcctggagaa a 51 <210> 220 <211> 51 <212> DNA <213> Bos taurus <400> 220 ggattctgtt tcccagggat ccccaatgct catcacggac ttgttctggg t 51 <210> 221 <211> 51 <212> DNA <213> Bos taurus <400> 221 tgcctgtagc tatgataatg atgtaatttt tgagtattaa taagctgatg a 51 <210> 222 <211> 51 <212> DNA <213> Bos taurus <400> 222 catttcctaa aagcctctca tttggggaat ttcttcttta tcaaagtcca g 51 <210> 223 <211> 51 <212> DNA <213> Bos taurus <400> 223 ggccgtatcc atgtagcgag ggtgaccata tatttttttc caaactacgt c 51 <210> 224 <211> 51 <212> DNA <213> Bos taurus <400> 224 agcatctact ctaatcaaac ataattggat tgtgtatgac ttaaaagaat g 51 <210> 225 <211> 51 <212> DNA <213> Bos taurus <400> 225 agtcatacac caatcttggt gagaaagagc cagtttctct tattattaat a 51 <210> 226 <211> 51 <212> DNA <213> Bos taurus <400> 226 ttgtttctta ggtgcttttt ataagattta acttcatatt cagtaagcta a 51 <210> 227 <211> 51 <212> DNA <213> Bos taurus <400> 227 atggatggga cctggccagg cattactatt acgtagagag atgcccttgg g 51 <210> 228 <211> 51 <212> DNA <213> Bos taurus <400> 228 ggagaaaccc cttcatcagt agactcagcc agttagctta tactgcggtc c 51 <210> 229 <211> 51 <212> DNA <213> Bos taurus <400> 229 gggagaaaaa ggtgccggtt gtccatggag aggacagaat ggttcaggca a 51 <210> 230 <211> 51 <212> DNA <213> Bos taurus <400> 230 ttgacggcct ttctgatatt tgtctactcc taaatggttg aatgagtaca a 51 <210> 231 <211> 51 <212> DNA <213> Bos taurus <400> 231 tagaaggggt tgaaatcttg aattcagttc aagtttttga ttccacctcc t 51 <210> 232 <211> 51 <212> DNA <213> Bos taurus <400> 232 aacacatgat ggattggtta attggatcaa gcatgtgaca gtccagggta g 51 <210> 233 <211> 51 <212> DNA <213> Bos taurus <400> 233 atacagtcct cacttctgtc ctgaacgtgt ttggagacaa gcctctctta t 51 <210> 234 <211> 51 <212> DNA <213> Bos taurus <400> 234 cacacacaaa tgtaatgctg atgaatgagc atctgaaatg ttgggaatgg a 51 <210> 235 <211> 51 <212> DNA <213> Bos taurus <400> 235 tgaaaaaact ttgaaggaag gagcagaaag attggcaatt gccattaggc t 51 <210> 236 <211> 51 <212> DNA <213> Bos taurus <400> 236 ccaataaaat tttaaactgc accttctggg tgaaggaccc agaagtccta a 51 <210> 237 <211> 51 <212> DNA <213> Bos taurus <400> 237 cggtagagtg cagtttaaag aaatatagat ttcacaaaac ctacaatttg t 51 <210> 238 <211> 51 <212> DNA <213> Bos taurus <400> 238 agacatattc cttccacgat cgtgcttctc ttcttctgtt ctgagggccc a 51 <210> 239 <211> 51 <212> DNA <213> Bos taurus <400> 239 gttctaacat gcagtgtggt gactaaataa aagtattgaa ctggacactt g 51 <210> 240 <211> 51 <212> DNA <213> Bos taurus <400> 240 ggaggaaagg acaaagaatt aaatgggagg ttttcacatt gtcctgaaag c 51 <210> 241 <211> 51 <212> DNA <213> Bos taurus <400> 241 agggatcact cacatgacta tagttctctg gtgactcagt aaaggatgat c 51 <210> 242 <211> 51 <212> DNA <213> Bos taurus <400> 242 cctagtcact catccattcc tcagaatggc tcctttataa tcctcaaaac t 51 <210> 243 <211> 51 <212> DNA <213> Bos taurus <400> 243 acttatgctt tttaagccta atctctccct atcttttctt cagagcccat t 51 <210> 244 <211> 51 <212> DNA <213> Bos taurus <400> 244 ttcccattct gcttctctca cctctcacat tggctatctt tattataagg t 51 <210> 245 <211> 51 <212> DNA <213> Bos taurus <400> 245 ggagagataa aaggaccagc gaccaacaga cactgagaac cattattgca a 51 <210> 246 <211> 51 <212> DNA <213> Bos taurus <400> 246 aagaatacaa ttctcaacaa actggacata aagaaaacaa acttcattaa a 51 <210> 247 <211> 51 <212> DNA <213> Bos taurus <400> 247 ctaattttca tggtttcatc ttttacgtat tcaaacattt catatgatta t 51 <210> 248 <211> 51 <212> DNA <213> Bos taurus <400> 248 agatgctgct agagttgatg cagaaggtaa gtttccttcc tgttaagcaa c 51 <210> 249 <211> 51 <212> DNA <213> Bos taurus <400> 249 tatgtcagac tccatcttct gtgagatctg gctctcccca gagagtactg g 51 <210> 250 <211> 51 <212> DNA <213> Bos taurus <400> 250 ctttttggag tacacagaga tgctacgttc ttcccaaatt gatacataga a 51 <210> 251 <211> 51 <212> DNA <213> Bos taurus <400> 251 cagtcaaccc aactgtgagg aaaatttcag aaattaaact agactaagtt a 51 <210> 252 <211> 51 <212> DNA <213> Bos taurus <400> 252 actgcagagc aaacactgct tgtgagagga gccctgcatt ggcagaaatg g 51 <210> 253 <211> 51 <212> DNA <213> Bos taurus <400> 253 acctaccact ttctaactgc atggccttga gcaagttttt taaaaccaca c 51 <210> 254 <211> 51 <212> DNA <213> Bos taurus <400> 254 ctttttggga ctcaaataac tctgacccaa aacgttttaa taaattaaac a 51 <210> 255 <211> 51 <212> DNA <213> Bos taurus <400> 255 ccagtatgca tctctgaatg gggccatgtg taaccccttg tggtactgaa t 51 <210> 256 <211> 51 <212> DNA <213> Bos taurus <400> 256 cagttagatc agaatctcaa agtgacgcct aggaatcagt atttttaaat t 51 <210> 257 <211> 51 <212> DNA <213> Bos taurus <400> 257 tctggtacag agaattttgt tttccacatg gaaaaattca cgcctcagtt t 51 <210> 258 <211> 51 <212> DNA <213> Bos taurus <400> 258 ctatgatgac tggtctccct gggaacgtac agcttagcta ctccatccga a 51 <210> 259 <211> 51 <212> DNA <213> Bos taurus <400> 259 ataaatagaa ctgaatttgt tttaggttgt ctaatttctc taccattaca g 51 <210> 260 <211> 51 <212> DNA <213> Bos taurus <400> 260 agcaataaaa tacaccagac acggtgcact aacaacttgg ataaatcttg a 51 <210> 261 <211> 51 <212> DNA <213> Bos taurus <400> 261 agaacaatgt cacacccata tatgtatgca tgtatatgat tgcagacagg g 51 <210> 262 <211> 51 <212> DNA <213> Bos taurus <400> 262 aaataatttt aatcattttt attacacttc atggaatctt taaatgtggt c 51 <210> 263 <211> 51 <212> DNA <213> Bos taurus <400> 263 aagatttaaa taagaagttt gttcatttat tgagcaatga tttatcgaat g 51 <210> 264 <211> 51 <212> DNA <213> Bos taurus <400> 264 tggctttggc acttgaaatg tgtcttgtct taactgagat gtgctataaa t 51 <210> 265 <211> 51 <212> DNA <213> Bos taurus <400> 265 catagagatc atgtgctcat ctgttgcaaa tggcctgata tttcctactg t 51 <210> 266 <211> 51 <212> DNA <213> Bos taurus <400> 266 cccatccctt tccatcatcg ccctcgttct tggattggtc caatgcacag a 51 <210> 267 <211> 51 <212> DNA <213> Bos taurus <400> 267 gtatcatgct tgtttgcagg tacttttttt taatactctg agttgtgttt c 51 <210> 268 <211> 51 <212> DNA <213> Bos taurus <400> 268 taggccattt tcagatactg cagtagcagg aattatgatt cgtctatcca t 51 <210> 269 <211> 51 <212> DNA <213> Bos taurus <400> 269 cacttttaat catctaattg caactgatca ggaccctaca ggaccttccc a 51 <210> 270 <211> 51 <212> DNA <213> Bos taurus <400> 270 ctgtggctag aatgaggaaa tatgtttcat actgatacat atcatacaga t 51 <210> 271 <211> 51 <212> DNA <213> Bos taurus <400> 271 ccaggtcctc acagtggaga accagggaag actcccttgc ggtcctggca c 51 <210> 272 <211> 51 <212> DNA <213> Bos taurus <400> 272 ggtacatgat tactaaagga tcttacaaca aataatcaga cctcatttag t 51 <210> 273 <211> 51 <212> DNA <213> Bos taurus <400> 273 tggatagaag gatggaagca tgcacgcata gatggataca tgcaaggatg g 51 <210> 274 <211> 51 <212> DNA <213> Bos taurus <400> 274 tcactgttta taagtgttaa gcacagaaaa taagattgag acaaatttta a 51 <210> 275 <211> 51 <212> DNA <213> Bos taurus <400> 275 cttcagactt ccatagtttc ttaaccagaa gtcagtgtat gttcttattt t 51 <210> 276 <211> 51 <212> DNA <213> Bos taurus <400> 276 tataaccagt tgtcagcaaa acctctgacc taaaggacat cgagtggata g 51 <210> 277 <211> 51 <212> DNA <213> Bos taurus <400> 277 cttaaccact agtgccgtca ccgagcgctg gattatagag ttggcctcag c 51 <210> 278 <211> 51 <212> DNA <213> Bos taurus <400> 278 tgggttctgc cctggccttc aggagtacct gctctaggat cacccagagg t 51 <210> 279 <211> 51 <212> DNA <213> Bos taurus <400> 279 tgtggacact cttgccctct gtacatacac ccacatacat gcatccgtgc t 51 <210> 280 <211> 51 <212> DNA <213> Bos taurus <400> 280 acacgctttc tgatccttga atccattttt cattaactgt tcacattaag c 51 <210> 281 <211> 51 <212> DNA <213> Bos taurus <400> 281 aaaaactgca caacaaaaac ttagctactc gaggctcctg ctggctgtat c 51 <210> 282 <211> 51 <212> DNA <213> Bos taurus <400> 282 ctcagcgcaa cagaataaat gcccacttct cttcaaaatg taactgaggt t 51 <210> 283 <211> 51 <212> DNA <213> Bos taurus <400> 283 tgttagacta gtgtagagcc tcggaagcgt ccggtttcac accattattt t 51 <210> 284 <211> 51 <212> DNA <213> Bos taurus <400> 284 ccacagtgag aagcagtgca ctgcagtgga gagtagccca atcactccgc a 51 <210> 285 <211> 51 <212> DNA <213> Bos taurus <400> 285 gttttcagct gccctaagaa aggcatgctc aggtaatgac atcgtggaca t 51 <210> 286 <211> 51 <212> DNA <213> Bos taurus <400> 286 aaaggatccc ggaggtcctg ggtggttttc actccctata agtctgttgt c 51 <210> 287 <211> 51 <212> DNA <213> Bos taurus <400> 287 tatacccatt aacaggctac cagaacggca ggagttgagt gagcaaggac a 51 <210> 288 <211> 51 <212> DNA <213> Bos taurus <400> 288 aagttatgtc tacttgtgcc ctctgacttt gctctaccta aaaggcacta a 51 <210> 289 <211> 51 <212> DNA <213> Bos taurus <400> 289 ccatagctgc ccttccacgg ttgggcgtga agggtgacca tggagagaac a 51 <210> 290 <211> 51 <212> DNA <213> Bos taurus <400> 290 tgttcagctt ccacatctaa caatgcctcc ctcagcccac ctcacaagag g 51 <210> 291 <211> 51 <212> DNA <213> Bos taurus <400> 291 ctctgcctct ggactgtgct tccttgcagc acctgtgccc aggccccatc a 51 <210> 292 <211> 51 <212> DNA <213> Bos taurus <400> 292 ccctaacaga ctcagcccct gtcccatgtc ctctgctggg tgcttctggg a 51 <210> 293 <211> 51 <212> DNA <213> Bos taurus <400> 293 tggaggttct gaaaagaagg cattcgtctg tcatctgtgc acacgctcca g 51 <210> 294 <211> 51 <212> DNA <213> Bos taurus <400> 294 ctctaaattc gcagcgtccg cacagatctg tgcgtgtgtt catcctccaa t 51 <210> 295 <211> 51 <212> DNA <213> Bos taurus <400> 295 ttcaggcctc ccaccttgca gaactgtgag aaatcaatga ttatatttta a 51 <210> 296 <211> 51 <212> DNA <213> Bos taurus <400> 296 catgatgagt gctgtcagac agaggtctga tggtgcagga atcccacttt t 51 <210> 297 <211> 51 <212> DNA <213> Bos taurus <400> 297 agatgtccac cagagcttgc gcactctcct ctgacacagg ggtgcagttc t 51 <210> 298 <211> 51 <212> DNA <213> Bos taurus <400> 298 ggattgtctt cttccatagc ggcgcgacca tgttaatgaa cttactcttt t 51 <210> 299 <211> 51 <212> DNA <213> Bos taurus <400> 299 tggaatccaa cgctctgatc tttgcgcagc tggtcacgct catcattttg g 51 <210> 300 <211> 51 <212> DNA <213> Bos taurus <400> 300 acggagatgg tcaggtgtgg gtccaaactg ccttagagct caggaatgtc t 51 <210> 301 <211> 51 <212> DNA <213> Bos taurus <400> 301 tcaagcccat gaaggtggat aggagacctg gaagcggcgt ggccaagatc c 51 <210> 302 <211> 51 <212> DNA <213> Bos taurus <400> 302 ttctgctagg ataacactta cctaactgag tcttatagca ggcccctgac a 51 <210> 303 <211> 51 <212> DNA <213> Bos taurus <400> 303 tataagcaag agcaggtttt gagatttgaa aggtagagag aggaccaggt g 51 <210> 304 <211> 51 <212> DNA <213> Bos taurus <400> 304 tggtgggagg cgggccagag cggtcaccat aggaaccaca taaccatcac g 51 <210> 305 <211> 51 <212> DNA <213> Bos taurus <400> 305 ttccacaggc gagctacatg cccacgtgca aaaatgagtt taaggaaggc t 51 <210> 306 <211> 51 <212> DNA <213> Bos taurus <400> 306 gaggccaaaa aaactgaagt gctttgttct cacagtatta aaattagtga c 51 <210> 307 <211> 51 <212> DNA <213> Bos taurus <400> 307 taggtacaca gtatcacaat tctctcattt gatttgcctc acgcaataac a 51 <210> 308 <211> 51 <212> DNA <213> Bos taurus <400> 308 taaaaatcca actagtgact gcagagaggg gcttcagata gaagccctga c 51 <210> 309 <211> 51 <212> DNA <213> Bos taurus <400> 309 ccctgaggcc cagtaccttc ctcccaaccc cgtggcacca gagtctcagc c 51 <210> 310 <211> 51 <212> DNA <213> Bos taurus <400> 310 gagggcctga gggacagcaa tgaagagtca aggctgcagg ggaagggagg t 51 <210> 311 <211> 51 <212> DNA <213> Bos taurus <400> 311 ctcaaccctc ctgcgccagg tcaggtctac ttgtccaaag tgccccctac c 51 <210> 312 <211> 51 <212> DNA <213> Bos taurus <400> 312 caccaaggac tgaccctgca gccaccgcaa acagcaggta tctttccttt a 51 <210> 313 <211> 51 <212> DNA <213> Bos taurus <400> 313 agcatatggc caggttctgg gatatcatgg ctgtaaatga gggcccattg t 51 <210> 314 <211> 51 <212> DNA <213> Bos taurus <400> 314 ctgaatgtga ctgggtgtca tcaggctccc tgcagcaggt attggaggta c 51 <210> 315 <211> 51 <212> DNA <213> Bos taurus <400> 315 aacactaagg acgcctcagc ctcaccgaaa ccccggaaaa tgtcagcaga g 51 <210> 316 <211> 51 <212> DNA <213> Bos taurus <400> 316 ctccacccac atggagaatc tgtctatgtg ctccagccac agttgctggt c 51 <210> 317 <211> 51 <212> DNA <213> Bos taurus <400> 317 ggcccccatg tatactctga gagccattcg ctcactagag cgtgtcagtt c 51 <210> 318 <211> 51 <212> DNA <213> Bos taurus <400> 318 tgtgcctcct ctatggctgt taccagctgt gacagtctcc ttggcttgat c 51 <210> 319 <211> 51 <212> DNA <213> Bos taurus <400> 319 cagctgctcg tccagatgtg attgcttttg ctagagtaga ataacaggtg c 51 <210> 320 <211> 51 <212> DNA <213> Bos taurus <400> 320 aataccaaac aatgagccca agaacactga gagaaaattc acgccacatg c 51 <210> 321 <211> 51 <212> DNA <213> Bos taurus <400> 321 tacaagttct tagatttcaa gtccatcagt gatagtccag ccttgctcta t 51 <210> 322 <211> 51 <212> DNA <213> Bos taurus <400> 322 ttcatcttca cagcaacctc tgctacgggc tctacttcta atcctgtgtt t 51 <210> 323 <211> 51 <212> DNA <213> Bos taurus <400> 323 agtgggcctt ccagaccttt cctcctttgc tacccttgtt actgaagctc t 51 <210> 324 <211> 51 <212> DNA <213> Bos taurus <400> 324 gaatcacgtg gctattctgg cattactgag tctacattct gcaccgtgtg t 51 <210> 325 <211> 51 <212> DNA <213> Bos taurus <400> 325 actcacccac agatttggga ccctctcttc attgaggaca gaaggccaaa g 51 <210> 326 <211> 51 <212> DNA <213> Bos taurus <400> 326 caggccagcc cctcaagggt ctgggtgcag cagaggggac agggatgtcc t 51 <210> 327 <211> 51 <212> DNA <213> Bos taurus <400> 327 gctgattggt cccaggtgca actgagtcta acctgagctt tctaggaaga t 51 <210> 328 <211> 51 <212> DNA <213> Bos taurus <400> 328 aattcaggaa atcttcacta aggaacgtcc atgtggatga tcccaggagt a 51 <210> 329 <211> 51 <212> DNA <213> Bos taurus <400> 329 gttctataca tgggctttaa catattccct tctctttttc atattctcga t 51 <210> 330 <211> 51 <212> DNA <213> Bos taurus <400> 330 gaatgaatag gactatccac tgactctcca ccgtccctcg gctcagaaac t 51 <210> 331 <211> 51 <212> DNA <213> Bos taurus <400> 331 agctaattgg gagtcaacat cattactctt ttggttccca actggcttgg t 51 <210> 332 <211> 51 <212> DNA <213> Bos taurus <400> 332 ggccctgagg acaggtattc ttggcggctg cagcatgagc acaagggcaa a 51 <210> 333 <211> 51 <212> DNA <213> Bos taurus <400> 333 tttttgttat ggatctcacg ggtggagagt gggcgccctg ctgatttctg g 51 <210> 334 <211> 51 <212> DNA <213> Bos taurus <400> 334 atttccttcc tagcacactg atcttcctgc tcagttgttt tccacatatt g 51 <210> 335 <211> 51 <212> DNA <213> Bos taurus <400> 335 tgattaattg ttccccaaag caaagtggat ttggatcata gacttatagc t 51 <210> 336 <211> 51 <212> DNA <213> Bos taurus <400> 336 tacgaaggct tttcctccca tcctataaga ctagtgaaaa cgtgaggagc a 51 <210> 337 <211> 51 <212> DNA <213> Bos taurus <400> 337 ggggccatag gttatgcttt atgatcaggt gaggtggtta cctggacatt c 51 <210> 338 <211> 51 <212> DNA <213> Bos taurus <400> 338 gtttaatttt tatcttaatt cttctttggg gctctctgaa tttactagat c 51 <210> 339 <211> 51 <212> DNA <213> Bos taurus <400> 339 aggcgacact ccactcactg ccctcacaag cgtcggcaca gctgccaact g 51 <210> 340 <211> 51 <212> DNA <213> Bos taurus <400> 340 caagagtggg tgtgacttcc gcagctcctg cactcagtcc ttccaaggct c 51 <210> 341 <211> 51 <212> DNA <213> Bos taurus <400> 341 gcattgagtt aggcctcttc tttacgaaat tgctttgcat catctacagt g 51 <210> 342 <211> 51 <212> DNA <213> Bos taurus <400> 342 tgaaatattt atcaaatgaa taagcgatcg ttcccaagta tttccaggtg t 51 <210> 343 <211> 51 <212> DNA <213> Bos taurus <400> 343 actaaaacgc tgtattagcc agccttctta gctgggaggc acagccatgt g 51 <210> 344 <211> 51 <212> DNA <213> Bos taurus <400> 344 ggagtgtccc cctgaattac ctcaacagac ccctgcgaca gctctatgga c 51 <210> 345 <211> 51 <212> DNA <213> Bos taurus <400> 345 gcccacaaaa gaaaccccct acccactggc aatcactccc tattcctctc t 51 <210> 346 <211> 51 <212> DNA <213> Bos taurus <400> 346 gtgaggagta ggatttcagt ggacatgttt gtggacatca agatctccac t 51 <210> 347 <211> 51 <212> DNA <213> Bos taurus <400> 347 ggagactcca ggaagtgcct gccccgtcca gtccctcaca agcccctcaa c 51 <210> 348 <211> 51 <212> DNA <213> Bos taurus <400> 348 aacttccctg gttgagagat cagaaaggga agctgtcatg ctctgtgaag a 51 <210> 349 <211> 51 <212> DNA <213> Bos taurus <400> 349 tcctggtttt tccttctggg ctgaacatcc tcaccctgct ggctcaggtc c 51 <210> 350 <211> 51 <212> DNA <213> Bos taurus <400> 350 aaactacagc tcccagcttc tggctgccat tctagctcca catggccaca g 51 <210> 351 <211> 51 <212> DNA <213> Bos taurus <400> 351 acctcatata gcccaactgt tagttcaaaa ctaacagtgt aaggactccc t 51 <210> 352 <211> 51 <212> DNA <213> Bos taurus <400> 352 ccagaacaag cctctcagct gtagcgagcc tccttcagcc tggagtgaga g 51 <210> 353 <211> 51 <212> DNA <213> Bos taurus <400> 353 attccattct atagtgtaga agcactgcca tattggccac caggatcatg t 51 <210> 354 <211> 51 <212> DNA <213> Bos taurus <400> 354 tgctggagtg aaggacggat atgctctgtg cccactgggc tcacggctaa c 51 <210> 355 <211> 51 <212> DNA <213> Bos taurus <400> 355 gttgaaccaa agaggatatt aaagatagca gggcaatggc tccctactgc c 51 <210> 356 <211> 51 <212> DNA <213> Bos taurus <400> 356 aaaagaaaag actgaagaaa tgagaaaaga attcctggta acatgtaagt c 51 <210> 357 <211> 51 <212> DNA <213> Bos taurus <400> 357 ttcaggaccg actgcctgat ggacagacag acagagggat gattgcagca t 51 <210> 358 <211> 51 <212> DNA <213> Bos taurus <400> 358 caccaagcct atacatcttc cttttaatac tgatcactag tatcccccta g 51 <210> 359 <211> 51 <212> DNA <213> Bos taurus <400> 359 gcccagcact catctctcag tatttggctt cttgagacat gagcagccaa g 51 <210> 360 <211> 51 <212> DNA <213> Bos taurus <400> 360 aagatcccca aatcccagga gtacctatgg cacacattcc agagctccct g 51 <210> 361 <211> 51 <212> DNA <213> Bos taurus <400> 361 catggtgcta gctatcctgc attagcatct ccggtgctca caagggcttc c 51 <210> 362 <211> 51 <212> DNA <213> Bos taurus <400> 362 atagttacca tctagtttta atatagtctt actccatagc ctgacatttt a 51 <210> 363 <211> 51 <212> DNA <213> Bos taurus <400> 363 ggagaaaact ccagccaccc aaggggcttc actaacgagc tccactcaat a 51 <210> 364 <211> 51 <212> DNA <213> Bos taurus <400> 364 aaccataggt aattcaaggc cttgcctggc aggcctcatg aggagaaact g 51 <210> 365 <211> 51 <212> DNA <213> Bos taurus <400> 365 ggctcaacca gtgtgcacca caaccgtgct gttaaatgca gaaatactgt c 51 <210> 366 <211> 51 <212> DNA <213> Bos taurus <400> 366 gagggccact ggcagagtgg gggaattgag gctgacaaag atggcaggaa g 51 <210> 367 <211> 51 <212> DNA <213> Bos taurus <400> 367 gttgggattt ccaaaacctg tgagctccaa ctggacactt tgtatggcac g 51 <210> 368 <211> 51 <212> DNA <213> Bos taurus <400> 368 tcctaggctg tcagctggca ggcctgtgca gacttggctt gtgtccttgt g 51 <210> 369 <211> 51 <212> DNA <213> Bos taurus <400> 369 tcagagctgc ttctgacatg tctttgtact agagaatcaa ttgaccctaa g 51 <210> 370 <211> 51 <212> DNA <213> Bos taurus <400> 370 gttttgacta tgttgtctgc ttcctgtgtt caacttctac tgatgtacgc t 51 <210> 371 <211> 51 <212> DNA <213> Bos taurus <400> 371 tttagagtaa ctggcaggca tcagcacaat ttctcttaat acttcagcat a 51 <210> 372 <211> 51 <212> DNA <213> Bos taurus <400> 372 ccataatttg gtcatggatc agaaacgatg aaaagagaca tacttgaaat t 51 <210> 373 <211> 51 <212> DNA <213> Bos taurus <400> 373 cctcttaacc cctacctttg tcttatgaga tcttacacag tctttatgct a 51 <210> 374 <211> 51 <212> DNA <213> Bos taurus <400> 374 cggtatagac agtaagatcc tttctcgagc agtgccttgc ggcagagtaa a 51 <210> 375 <211> 51 <212> DNA <213> Bos taurus <400> 375 gttgcaacag cttctttact atcttatctt atttctttgc cataatagtc t 51 <210> 376 <211> 51 <212> DNA <213> Bos taurus <400> 376 cagaaactcc cctttagagc tctgagcagc agggggaggc tggtctttgg g 51 <210> 377 <211> 51 <212> DNA <213> Bos taurus <400> 377 tcctgatcaa tttccatctc tctaaatact cacatgttca atgaacacaa c 51 <210> 378 <211> 51 <212> DNA <213> Bos taurus <400> 378 tagctaattg tgtcaaattt gaagatgctg tactccgcgt cctctccaca c 51 <210> 379 <211> 51 <212> DNA <213> Bos taurus <400> 379 tgggagatgg tctaggaaaa agaagcatct gcacctctgt taccgcgaga c 51 <210> 380 <211> 51 <212> DNA <213> Bos taurus <400> 380 gcctgaccaa cggcaacctg acccctacta taacttctgt ctctactctc c 51 <210> 381 <211> 51 <212> DNA <213> Bos taurus <400> 381 gcagagctga ggcatgggaa gctgaggcat gaggagactg agcctcatgt c 51 <210> 382 <211> 51 <212> DNA <213> Bos taurus <400> 382 gaatttataa ggtggatgtt ttcaggttca tgtattgctg agagtgagct a 51 <210> 383 <211> 51 <212> DNA <213> Bos taurus <400> 383 acgaggccat gggccgcgag gtgacagcgc tcaagagcaa gctcaggtga g 51 <210> 384 <211> 51 <212> DNA <213> Bos taurus <400> 384 agagcttaga aaaagttaac tactgttctt gttacatttt acaaatacat t 51 <110> SNP GENETICS INC. <120> SNP GENES SET FOR IDENTIFYING INDIVIDUAL AND BLOODLINE OF HANWOO <130> IPM-34417 <160> 384 <170> KopatentIn 1.71 <210> 1 <211> 51 <212> DNA <213> Bos taurus <400> 1 ccagggccta gatcgttttc tgcccagggg tggatccctg gccatcacca t 51 <210> 2 <211> 51 <212> DNA <213> Bos taurus <400> 2 tgtctccatc ctcccttcta gttctaatct ttaaacatac tgataagaag g 51 <210> 3 <211> 51 <212> DNA <213> Bos taurus <400> 3 tttgcagatg aggggatatt ttccaagaga gagttgcctt tcttgtctga a 51 <210> 4 <211> 51 <212> DNA <213> Bos taurus <400> 4 tcacattttt actttttagt atttcgtaat gattcaaagc agaactatct t 51 <210> 5 <211> 51 <212> DNA <213> Bos taurus <400> 5 ggaaattcaa aaagataaca cttttgctga gtgtttgaat cttgagttga a 51 <210> 6 <211> 51 <212> DNA <213> Bos taurus <400> 6 acagtgaacc atgacaagta attatcgtga cctttgaaat tttaatatat g 51 <210> 7 <211> 51 <212> DNA <213> Bos taurus <400> 7 gattggaaca atttagggag gcaagcgtga ttttctgcag ttccttcaac a 51 <210> 8 <211> 51 <212> DNA <213> Bos taurus <400> 8 cggaatctcg attaggatgt tcccatgtgt cttattaaaa ggctgccagg c 51 <210> 9 <211> 51 <212> DNA <213> Bos taurus <400> 9 atgtcttcaa tgaactaaac tgaaccaagc tattattact gaattgcaaa c 51 <210> 10 <211> 51 <212> DNA <213> Bos taurus <400> 10 tttttaggga aaccatacat tttctaactt ttgaaaaatg caagcactca g 51 <210> 11 <211> 51 <212> DNA <213> Bos taurus <400> 11 atttctcatt gtgcttgttt attcagcttg aacacttctg tgcattgggt a 51 <210> 12 <211> 51 <212> DNA <213> Bos taurus <400> 12 gatgatattc aggctttagc taagacttat tgtaaggcaa ccatgatctg t 51 <210> 13 <211> 51 <212> DNA <213> Bos taurus <400> 13 atgcacaacg tttctagaga cgccagtgtg aaggcaggtg ctttgcaacc t 51 <210> 14 <211> 51 <212> DNA <213> Bos taurus <400> 14 actaggcttg aatgcagttg ctgtggaatg cactaattaa ttacatttat t 51 <210> 15 <211> 51 <212> DNA <213> Bos taurus <400> 15 gacttatcac tctatcaagt ttatttatat ttgattcatc ccttacaact g 51 <210> 16 <211> 51 <212> DNA <213> Bos taurus <400> 16 atcatcagtt atcatctctg atttgatttt aatagaaaca atggagcctg t 51 <210> 17 <211> 51 <212> DNA <213> Bos taurus <400> 17 tgtgtattcc tatttaaaaa tgcctgccac gttatatgac ctcatgttaa t 51 <210> 18 <211> 51 <212> DNA <213> Bos taurus <400> 18 ttctgggtct tgaggatgtg aaaccgtcta ctgaaaccat atttgtaaat g 51 <210> 19 <211> 51 <212> DNA <213> Bos taurus <400> 19 tcgtgcaata gaaatacaag caatcttgta tattttggca gatagtgccc g 51 <210> 20 <211> 51 <212> DNA <213> Bos taurus <400> 20 cttgaggtga tttagatggg tgtctgaggc tatctttgga tgtacttttg t 51 <210> 21 <211> 51 <212> DNA <213> Bos taurus <400> 21 agtagggtcc aagaagcaga gagtagaagt gcaaaacctc aaaaatgatg a 51 <210> 22 <211> 51 <212> DNA <213> Bos taurus <400> 22 ctcttgagga atttgctgat gactttaaaa aaaaagcacc aaacactgta a 51 <210> 23 <211> 51 <212> DNA <213> Bos taurus <400> 23 caagtttaac acaaaagaaa attttaaaag cctgagtttt taaagtaggt g 51 <210> 24 <211> 51 <212> DNA <213> Bos taurus <400> 24 agtgggaata atgagaagca gttgcgttgt tttctatcat ttttaggttt t 51 <210> 25 <211> 51 <212> DNA <213> Bos taurus <400> 25 tgttctttcc aaagactctg tgggggtaca ttttcaatgt ggagagcaat a 51 <210> 26 <211> 51 <212> DNA <213> Bos taurus <400> 26 aggcagatga tatttcaagt taaacgtcaa gtatattgtc cttaccatca g 51 <210> 27 <211> 51 <212> DNA <213> Bos taurus <400> 27 tgactaaatt aaccccaccc cacccgcaat tctcttttta tcagtacctc t 51 <210> 28 <211> 51 <212> DNA <213> Bos taurus <400> 28 caaggcccag aaagaggcaa actggtactt ctggaccacc ataccagctg g 51 <210> 29 <211> 51 <212> DNA <213> Bos taurus <400> 29 aaacccacaa tatcacacac tcccatgtcc ttattccctc tgaaaagtct c 51 <210> 30 <211> 51 <212> DNA <213> Bos taurus <400> 30 tataattctg tgaaactcga gcttgacatt aatttttact ttttacaaag c 51 <210> 31 <211> 51 <212> DNA <213> Bos taurus <400> 31 cctcagcctg ctgctcacgg catgcgttcg ctatattctg cgttatttat t 51 <210> 32 <211> 51 <212> DNA <213> Bos taurus <400> 32 atgttgttag atgagtactc tctctttttt tttttttggc tatgtatcta g 51 <210> 33 <211> 51 <212> DNA <213> Bos taurus <400> 33 cggctcatga aggaatagtt tcttggcttc tcgagcccct tgttttggat g 51 <210> 34 <211> 51 <212> DNA <213> Bos taurus <400> 34 tagtatgtca aacatgaagg ttaaatcaac ctcgtgaatt aaaaaaattc c 51 <210> 35 <211> 51 <212> DNA <213> Bos taurus <400> 35 ctgagaagca ccctggcaca aggctggcct tctgcagctg atggccacct t 51 <210> 36 <211> 51 <212> DNA <213> Bos taurus <400> 36 agagactaga aaaaggcagg tctgtcgaat ctaaaacatt tggcaaactg a 51 <210> 37 <211> 51 <212> DNA <213> Bos taurus <400> 37 tgttaggagt aggagatgaa gtggacatac acaccattca ttatcatgct g 51 <210> 38 <211> 51 <212> DNA <213> Bos taurus <400> 38 tttttgtgct ttaaaaagaa aaaaaactct ctgagcttca aatgagagtt t 51 <210> 39 <211> 51 <212> DNA <213> Bos taurus <400> 39 tcaccagagg gcagcgctgt gccattgccc tctggttcac tttggatgct c 51 <210> 40 <211> 51 <212> DNA <213> Bos taurus <400> 40 ttattttcta gagtgttggt ggggttatgt tgatctactt acttcttttc c 51 <210> 41 <211> 51 <212> DNA <213> Bos taurus <400> 41 gtgtggtcag atgcagtgtg ccttgtgggt ataaaattaa tttagcaccg a 51 <210> 42 <211> 51 <212> DNA <213> Bos taurus <400> 42 gctactcttg ttttaagtaa attctgtgtc acatgtattc cttactgtta t 51 <210> 43 <211> 51 <212> DNA <213> Bos taurus <400> 43 gtacaacatc ataaataatg aaatactgta atgaaattgt atgggaacct a 51 <210> 44 <211> 51 <212> DNA <213> Bos taurus <400> 44 agcttcagtg cagaattaac agagctccct tgcttaggtt taatttggat a 51 <210> 45 <211> 51 <212> DNA <213> Bos taurus <400> 45 taacacctag actcatactt tggcctcagc agtacagagg atacaggtat t 51 <210> 46 <211> 51 <212> DNA <213> Bos taurus <400> 46 caggttgtgc tctgcaaatc acaaacgtta ggaagagcaa taattgcagt a 51 <210> 47 <211> 51 <212> DNA <213> Bos taurus <400> 47 tttgtagagt aaatactctt aaaccatttg tatgtagaca aacatctaac t 51 <210> 48 <211> 51 <212> DNA <213> Bos taurus <400> 48 tgctctgctt gttttgctag tcatcgtttg gttgattgaa taatgatgat g 51 <210> 49 <211> 51 <212> DNA <213> Bos taurus <400> 49 gaaccggctc cgcgtatatt tagttgtaat tcctaaactg aaaagtggct a 51 <210> 50 <211> 51 <212> DNA <213> Bos taurus <400> 50 ttaaaaaatg atcataaggg aaaatggaat gaatagttgg tttgggggta a 51 <210> 51 <211> 51 <212> DNA <213> Bos taurus <400> 51 tttgcttcat gaggagatta cactgaggta atacattctg aacgttgaaa c 51 <210> 52 <211> 51 <212> DNA <213> Bos taurus <400> 52 atttcacaga gaccacaaaa cccgcgtcag gtgtgaaagt gctttacctg a 51 <210> 53 <211> 51 <212> DNA <213> Bos taurus <400> 53 tcactaggat tcagtgaagc ctctggaact ccggctccgc gtggacaggg t 51 <210> 54 <211> 51 <212> DNA <213> Bos taurus <400> 54 tgtagttttt aaaatatcat aaagacgagt ctcacaaaag ggaatctttc a 51 <210> 55 <211> 51 <212> DNA <213> Bos taurus <400> 55 agctgatgag cagtgaaata aatctattca gtcaacggag attgggttta t 51 <210> 56 <211> 51 <212> DNA <213> Bos taurus <400> 56 agaaacagca tatcaaattt cttctcattt ctatctgtct cccagtgaaa t 51 <210> 57 <211> 51 <212> DNA <213> Bos taurus <400> 57 gccattcagg tctcctcctg agctccgtct ccagtgcatc acaagagtcc t 51 <210> 58 <211> 51 <212> DNA <213> Bos taurus <400> 58 tagtggtacc ctcactcctg ggctcgaaga gtcataataa tccccttcct t 51 <210> 59 <211> 51 <212> DNA <213> Bos taurus <400> 59 atatgatgac aaacaagaaa acaattataa agaaaaacta tagccaggag g 51 <210> 60 <211> 51 <212> DNA <213> Bos taurus <400> 60 ggtcccctcg ccaggccacc ccaacgctga gctatttggg gaattgcttc c 51 <210> 61 <211> 51 <212> DNA <213> Bos taurus <400> 61 ataagggcag agacctattt aattcttctc aacagtgcct agagttaagt a 51 <210> 62 <211> 51 <212> DNA <213> Bos taurus <400> 62 ctcttattta ctctcagagt atccatgaag ctgaagcttg tgatgggttt t 51 <210> 63 <211> 51 <212> DNA <213> Bos taurus <400> 63 catataaaat catcttgaga aagacgatgg atgggaaaga agacatcttt t 51 <210> 64 <211> 51 <212> DNA <213> Bos taurus <400> 64 gcttgtcttt aaaaaatgta tacagttaaa gcataataaa aatgccccaa t 51 <210> 65 <211> 51 <212> DNA <213> Bos taurus <400> 65 cagacataaa tactgctctc caggatcctc tgttcctttc agagttttga t 51 <210> 66 <211> 51 <212> DNA <213> Bos taurus <400> 66 taattgattc tactaactaa aacttgtcct cacatttggg attaaacttg a 51 <210> 67 <211> 51 <212> DNA <213> Bos taurus <400> 67 cttgcattgt tcttctcaaa aatgttggct cattcagtca cttctatcag g 51 <210> 68 <211> 51 <212> DNA <213> Bos taurus <400> 68 gcatttatta agtctctgag cccagcgccc tgaagtcaaa attctagaaa c 51 <210> 69 <211> 51 <212> DNA <213> Bos taurus <400> 69 ttctggaagc tgtactcttg ggcttggacc atgtgttgag acaaaacaga c 51 <210> 70 <211> 51 <212> DNA <213> Bos taurus <400> 70 taagcttgtg atatattgca gattatagaa tcctgtacct tcatagatct a 51 <210> 71 <211> 51 <212> DNA <213> Bos taurus <400> 71 taacagatta atactgtttc tgtcttcttc aattatacac tgtgcactta g 51 <210> 72 <211> 51 <212> DNA <213> Bos taurus <400> 72 cgtatggaaa cctggagaaa ggcccgggaa cacacagggc cagaggcatc c 51 <210> 73 <211> 51 <212> DNA <213> Bos taurus <400> 73 ttgcagctgt tttttatttg atcttttaaa gatccaaaga tgttcaagga t 51 <210> 74 <211> 51 <212> DNA <213> Bos taurus <400> 74 cagccgtaag actctccagc acatctatca gcttggcatt gctctctcat g 51 <210> 75 <211> 51 <212> DNA <213> Bos taurus <400> 75 ccacttttcc ctcgcacgct tcctcggtag cagccctatt tgcggatctt c 51 <210> 76 <211> 51 <212> DNA <213> Bos taurus <400> 76 acaggacgct tccctgagtg tagaccgagc tctacacagc tcttcagaag t 51 <210> 77 <211> 51 <212> DNA <213> Bos taurus <400> 77 actcgctccg gatcctagac tgcagcttca accgtatcat ggcctctaag g 51 <210> 78 <211> 51 <212> DNA <213> Bos taurus <400> 78 gaaatcaaga tatgaaatag caatatagga aacacaaaaa agctgagttt g 51 <210> 79 <211> 51 <212> DNA <213> Bos taurus <400> 79 tggccagccc gtgccgcgaa cacaccgagt aggggcagat ctcggctccc a 51 <210> 80 <211> 51 <212> DNA <213> Bos taurus <400> 80 gcaggggctg tgctctctgg gggacgtata gaagctgtct ttaccttcaa t 51 <210> 81 <211> 51 <212> DNA <213> Bos taurus <400> 81 tccttaaata taggagctta gtgcaagggt ttatttaaaa ctcacagtat a 51 <210> 82 <211> 51 <212> DNA <213> Bos taurus <400> 82 ttaattttgt atagcttaag cgcccatctc tttaatctat taaaatagag t 51 <210> 83 <211> 51 <212> DNA <213> Bos taurus <400> 83 tttctgttgc tggaaaggag gggcatgagg aactgaagca acagctctag g 51 <210> 84 <211> 51 <212> DNA <213> Bos taurus <400> 84 ttaaagacat tttccttaaa gactttctta taggggagtc catggcaaaa t 51 <210> 85 <211> 51 <212> DNA <213> Bos taurus <400> 85 aatatcaaaa gtatttttcc tgaccgttgt acaatgacac gagttcctca a 51 <210> 86 <211> 51 <212> DNA <213> Bos taurus <400> 86 ttgatgctac gttgaccgga ggccctgcca ggtgagaaga gggatgagca a 51 <210> 87 <211> 51 <212> DNA <213> Bos taurus <400> 87 tttcagtatc tgaacatgct atcaccgtgt aaaacagatt tctttattac a 51 <210> 88 <211> 51 <212> DNA <213> Bos taurus <400> 88 aaattctagt gctccatggc ttagattaac taccctagaa aagttctcca c 51 <210> 89 <211> 51 <212> DNA <213> Bos taurus <400> 89 agctttatta atgccataaa gctattgaaa gaaacgtggc ttacccttgt c 51 <210> 90 <211> 51 <212> DNA <213> Bos taurus <400> 90 cttcactttc ttcagaccaa ttctggttaa aagaaggaaa tacaccttaa c 51 <210> 91 <211> 51 <212> DNA <213> Bos taurus <400> 91 tctgcaggaa ggttatggga gcaccgtgtc agggagacgg cccagctcac a 51 <210> 92 <211> 51 <212> DNA <213> Bos taurus <400> 92 attttgtaaa atttccagct cagaagtccc tcagtgtttt gattattctc c 51 <210> 93 <211> 51 <212> DNA <213> Bos taurus <400> 93 atcatgatac aagtcactgt gttttgttgg aaatgcaaca aataaaattg c 51 <210> 94 <211> 51 <212> DNA <213> Bos taurus <400> 94 cttggtcctc cattgaggtg gacagttcct gcaccctggg gctgaatgag g 51 <210> 95 <211> 51 <212> DNA <213> Bos taurus <400> 95 acaaactcat tggcatcacc catcagtctt actcattatg gctcacagct g 51 <210> 96 <211> 51 <212> DNA <213> Bos taurus <400> 96 tatttttttc atattttaaa aacaatcaag cggacagact tgaaataata a 51 <210> 97 <211> 51 <212> DNA <213> Bos taurus <400> 97 cttaaaatat tgtgtaggac tcaccgctct tagctgttag aatgttgtta a 51 <210> 98 <211> 51 <212> DNA <213> Bos taurus <400> 98 agtagacggt cctgggtgtt gagtcgtatg gatgctagat tgcaataccc t 51 <210> 99 <211> 51 <212> DNA <213> Bos taurus <400> 99 attgtttaag tgcatattag aaagttaaaa atcatatatg tggctaccat t 51 <210> 100 <211> 51 <212> DNA <213> Bos taurus <400> 100 ataaattgaa tagtgtgtat atgtgtgttg gagcttgtag tagcctctga c 51 <210> 101 <211> 51 <212> DNA <213> Bos taurus <400> 101 ctgggccctg ggatatagct ttcccgttgg tgctgattct gtggtccttg c 51 <210> 102 <211> 51 <212> DNA <213> Bos taurus <400> 102 tagaagggcc tgctttacaa agtaccggct gataatgttt tccaatcaac a 51 <210> 103 <211> 51 <212> DNA <213> Bos taurus <400> 103 aaggcaagag gagggcttag attaggaaaa gtacaaacag aaaagtcaac t 51 <210> 104 <211> 51 <212> DNA <213> Bos taurus <400> 104 aaatatttcc ccccagaaag caagagggag agtctgttct tgtggagatc t 51 <210> 105 <211> 51 <212> DNA <213> Bos taurus <400> 105 cctccgtgta atacatcaag ttcaatgtcc accaatgaac caaactgcat g 51 <210> 106 <211> 51 <212> DNA <213> Bos taurus <400> 106 gatttgttgc ctttcagacc tggacggctg aatggccaac cttattccta a 51 <210> 107 <211> 51 <212> DNA <213> Bos taurus <400> 107 tcgccctttg gtctaacaat gccagtcttg acttgttgct gtttactaat t 51 <210> 108 <211> 51 <212> DNA <213> Bos taurus <400> 108 ctgccacggg attaaggttc atgccgtaga attaaaggct ttaaaatagc a 51 <210> 109 <211> 51 <212> DNA <213> Bos taurus <400> 109 tcagagcgtt gactaatagg gttaactgat agaagtccag tagtctggtt t 51 <210> 110 <211> 51 <212> DNA <213> Bos taurus <400> 110 aaagcaggag agaaatgtga acctgagaag gtgtttttcg cttgatttag a 51 <210> 111 <211> 51 <212> DNA <213> Bos taurus <400> 111 tgcgtctaaa aatacccgcc ttgggaaatg taaaggtttc ccctgactcc t 51 <210> 112 <211> 51 <212> DNA <213> Bos taurus <400> 112 aagacccaga ctagatgcta catctactaa ggagtcatgt ctaatgtgta c 51 <210> 113 <211> 51 <212> DNA <213> Bos taurus <400> 113 agtaaggcac tggataagtg gggttcgtct gtatagtgga aaactaagca g 51 <210> 114 <211> 51 <212> DNA <213> Bos taurus <400> 114 tcttcttgta ttaatttctc tctttttcca ctcctccaaa accttcaggt a 51 <210> 115 <211> 51 <212> DNA <213> Bos taurus <400> 115 cccacacaaa gggagttctt aatctattac tattacgctg gtgacaatga a 51 <210> 116 <211> 51 <212> DNA <213> Bos taurus <400> 116 gggggttcgt tatgtgaggc aatgcgttgt gtgctctaat cttttctggt a 51 <210> 117 <211> 51 <212> DNA <213> Bos taurus <400> 117 gaaaatccat tgagtgtcat gcagtggtgg gaatggaaat aatcttcttt g 51 <210> 118 <211> 51 <212> DNA <213> Bos taurus <400> 118 gaatataaaa ttgctcgcca tcctctacga atattccttt catacgggta a 51 <210> 119 <211> 51 <212> DNA <213> Bos taurus <400> 119 ggcagtcgag agcctggggc accagtgacg tccaggtcct ctttacccac c 51 <210> 120 <211> 51 <212> DNA <213> Bos taurus <400> 120 tgtgtgtttc atgtctcact ttaacgtgta cctcatcctc atcatgtgca a 51 <210> 121 <211> 51 <212> DNA <213> Bos taurus <400> 121 tcagatgaca atgagaagtg gtacttggtg aggagagtca atgtgacaag g 51 <210> 122 <211> 51 <212> DNA <213> Bos taurus <400> 122 tgtctacagc agaggcaaag acaagtgggg gagaagaatg tttctcaggc a 51 <210> 123 <211> 51 <212> DNA <213> Bos taurus <400> 123 tagaagatac atcagtcatg gatacggtgt tgaggaggaa gaaagcagaa a 51 <210> 124 <211> 51 <212> DNA <213> Bos taurus <400> 124 cttctctgaa gcctctctgt accctaaaca gttgactgtg ggtggagagg t 51 <210> 125 <211> 51 <212> DNA <213> Bos taurus <400> 125 gaaagagata gatgctattt ttacccacat agagcttact gtcagtggtg a 51 <210> 126 <211> 51 <212> DNA <213> Bos taurus <400> 126 tggtgctggc catagttcac ttttatagca atccattaag acagacatta t 51 <210> 127 <211> 51 <212> DNA <213> Bos taurus <400> 127 aactcaatca ggtacaaggc catgcggact tcattctcta aaggatcttt c 51 <210> 128 <211> 51 <212> DNA <213> Bos taurus <400> 128 gattaaaaaa gaatagagaa agtcacgagt gtgcactgat tgcaaaaaag a 51 <210> 129 <211> 51 <212> DNA <213> Bos taurus <400> 129 cagatttttc ttttaactgc tatccgtgtg gcattacatt gtggatattc c 51 <210> 130 <211> 51 <212> DNA <213> Bos taurus <400> 130 agagaaaggt tgggtgagta ggaatattta taacttggcg acaggggaga t 51 <210> 131 <211> 51 <212> DNA <213> Bos taurus <400> 131 acacaaatga acgactactt tacttcactt taatatgacc atcagttaag a 51 <210> 132 <211> 51 <212> DNA <213> Bos taurus <400> 132 tgacactgaa accgcagtat ttgtggattc tgctttcaac tagaatagtc c 51 <210> 133 <211> 51 <212> DNA <213> Bos taurus <133> 133 tctcttttct ttagcataat cgctactgct atattttcag gttcaatttt a 51 <210> 134 <211> 51 <212> DNA <213> Bos taurus <400> 134 ctccctcctt tctctttgta gtggaggaag tggaattcat aattcatttg a 51 <210> 135 <211> 51 <212> DNA <213> Bos taurus <400> 135 accactgagt ctgagctgcc acacaatttt gtcatttcct ttatcatgtc c 51 <210> 136 <211> 51 <212> DNA <213> Bos taurus <400> 136 atgttttatg tttaatgtta ttgctcataa ccgatttttt tagggaacat t 51 <210> 137 <211> 51 <212> DNA <213> Bos taurus <400> 137 cacaccatga cccattatag ctcatttgac aaagagaata aagatgcctg c 51 <210> 138 <211> 51 <212> DNA <213> Bos taurus <400> 138 gtacagtatt atttcagcaa catcaattct tacaggcttc tttgaaaatt t 51 <139> <211> 51 <212> DNA <213> Bos taurus <400> 139 cattttagta tcaattttta aagcactatg cgtcatcgca atttaccata t 51 <210> 140 <211> 51 <212> DNA <213> Bos taurus <400> 140 caaaatcccc atcgggaggc actgatggaa cagcaactag gaaggcacac a 51 <210> 141 <211> 51 <212> DNA <213> Bos taurus <400> 141 caactttgtt ggtggtatat tttacatatc ctgagattta tttattgcag g 51 <210> 142 <211> 51 <212> DNA <213> Bos taurus <400> 142 atctcaataa agactcagaa aaacaccctg aggttgcaac actgtaaatt t 51 <210> 143 <211> 51 <212> DNA <213> Bos taurus <400> 143 taagttgccc agagaaaaag ggacattgta gtccggtttt ctaatctttg t 51 <210> 144 <211> 51 <212> DNA <213> Bos taurus <400> 144 tgctttctct agaatggggt gtgaatcagt agaatttgtg ttgtttctca g 51 <210> 145 <211> 51 <212> DNA <213> Bos taurus <400> 145 atacactctc ttctccagcc tactcggcta cacctcatgt ctgactccgc t 51 <210> 146 <211> 51 <212> DNA <213> Bos taurus <400> 146 acaggcatag ctggttcctc ctgagtacca tgagctgccc caggccccct c 51 <210> 147 <211> 51 <212> DNA <213> Bos taurus <400> 147 ttaggacttc ggttatcaaa atccagtcaa aattccactt taattttgat c 51 <210> 148 <211> 51 <212> DNA <213> Bos taurus <400> 148 tcactatcat attttatttc ctcaggtaaa aattacaatt gataaagaag a 51 <210> 149 <211> 51 <212> DNA <213> Bos taurus <400> 149 tcctcattaa atataggctc tgtgagtata agcatgtttg tctgcttctt t 51 <210> 150 <211> 51 <212> DNA <213> Bos taurus <400> 150 actgagatgc acagtactgg gccccgccct aggtcttctg aattagaatt t 51 <210> 151 <211> 51 <212> DNA <213> Bos taurus <400> 151 caatataact gtccttgaaa aacaatgata aatggacata attgtcaaag a 51 <210> 152 <211> 51 <212> DNA <213> Bos taurus <400> 152 aatgcccaag cacatgaaag agcctggcat caggacttca aagaaatctg t 51 <210> 153 <211> 51 <212> DNA <213> Bos taurus <400> 153 tttattcagc tcactaggat attttcctaa attgacccaa tgaggacata g 51 <210> 154 <211> 51 <212> DNA <213> Bos taurus <400> 154 tttgacataa ttactgtgga tttcctgaac cactcttatg ttatatgcct a 51 <210> 155 <211> 51 <212> DNA <213> Bos taurus <400> 155 tatgaccatg ctgggcatca aggctgaccc agaaagcagt ctgagagcag c 51 <210> 156 <211> 51 <212> DNA <213> Bos taurus <400> 156 tgtacagtta gtgaagtttg gtatagttca gcttttcttt agatcttcca t 51 <210> 157 <211> 51 <212> DNA <213> Bos taurus <400> 157 tctaagaaat agtacagagg gatcctgtga accctttacc cagttttctc a 51 <210> 158 <211> 51 <212> DNA <213> Bos taurus <400> 158 tgttttcagc ttcaagtact ggtgtcttac acgtacccat taactaaaca t 51 <210> 159 <211> 51 <212> DNA <213> Bos taurus <400> 159 agcatgtctg tgttgaactt cagtagtgat gaatatctag aaagcatttt c 51 <210> 160 <211> 51 <212> DNA <213> Bos taurus <400> 160 gatacagatt ctcaggtcct gacacaggag tttggtcctg acactgagtt g 51 <210> 161 <211> 51 <212> DNA <213> Bos taurus <400> 161 tgaggaaaca gaagtagaaa ctagcgttat tttataaatg atcccaaaac c 51 <210> 162 <211> 51 <212> DNA <213> Bos taurus <400> 162 cttgttatct tatatgctgg gcttcgatag aattttccct tacccaggta g 51 <210> 163 <211> 51 <212> DNA <213> Bos taurus <400> 163 taattaaaaa ccataagtta aaacctctgt ctccagaaac ttaaaaccat t 51 <210> 164 <211> 51 <212> DNA <213> Bos taurus <400> 164 tggctttgcc aaggagggga gctccagaaa ccagtgtgat ggtcactgag t 51 <210> 165 <211> 51 <212> DNA <213> Bos taurus <400> 165 aacataactc agaccactgg ctaaaacact ggtagtctta gaacaatgta a 51 <210> 166 <211> 51 <212> DNA <213> Bos taurus <400> 166 ttctcctgac tcaggcacca atatctagac tttgtctttt gttggcccag a 51 <210> 167 <211> 51 <212> DNA <213> Bos taurus <400> 167 gttgggctaa gattatatca agatttaggt ccaggactgt gaattcaagg c 51 <210> 168 <211> 51 <212> DNA <213> Bos taurus <400> 168 agagaggctg agtagagaac tgaggcctat gatatatgac tgtaatcatt c 51 <210> 169 <211> 51 <212> DNA <213> Bos taurus <400> 169 aaaatggtgg ctcttttatg ccactaagtt tttggaaggt ttgttgtgca g 51 <210> 170 <211> 51 <212> DNA <213> Bos taurus <400> 170 gatacattag tagagatcgc atggccgcag attttaaaca cattggtaga t 51 <210> 171 <211> 51 <212> DNA <213> Bos taurus <400> 171 ttgggatttt atatagtgcc catggctgac tttagaaatt aggggaaata a 51 <210> 172 <211> 51 <212> DNA <213> Bos taurus <400> 172 gtagagtaaa agaattttgg agatgtttaa actaaacacc ttgtgagaga g 51 <210> 173 <211> 51 <212> DNA <213> Bos taurus <400> 173 tagaatttat gccattgctt aacatgagaa ttttttttgg tcagctttaa a 51 <210> 174 <211> 51 <212> DNA <213> Bos taurus <400> 174 ggaaagcacc tgtcaataag gaccacgcaa ctgtattttt tgccctgcat a 51 <175> 175 <211> 51 <212> DNA <213> Bos taurus <400> 175 ggaaaagtgc ttaattaaca ctgactagtt ttaacctcct caagtaaggc c 51 <210> 176 <211> 51 <212> DNA <213> Bos taurus <400> 176 ttccttggaa agaaatgagt catctcggta aaaaatatac attaaagcat g 51 <210> 177 <211> 51 <212> DNA <213> Bos taurus <400> 177 gaggtcagtt cctccaaggg actctcggac cttttatttc aggcttgttg t 51 <210> 178 <211> 51 <212> DNA <213> Bos taurus <400> 178 acagggatgt cttgctccct cattgcacag ccccaagctc ctcgaggccc t 51 <210> 179 <211> 51 <212> DNA <213> Bos taurus <400> 179 ctagtgtgtt tgattaaaaa gtgcctcatg agatcctcct aaaatccaaa a 51 <210> 180 <211> 51 <212> DNA <213> Bos taurus <400> 180 cgggacctgg tcacagctcc gtcgccgagt gtctgggtga tgacgtgtcc c 51 <210> 181 <211> 51 <212> DNA <213> Bos taurus <400> 181 gggaaaaaac cagttcagct gaacgtgccc tgctagctct ctgtggaggg c 51 <210> 182 <211> 51 <212> DNA <213> Bos taurus <400> 182 gggctagaac ccgacaagag aagcctccgc aatgagaagc ccacgcatgt a 51 <210> 183 <211> 51 <212> DNA <213> Bos taurus <400> 183 cccagaacag tggagatact tagtactcct gatgcctgta ggcaaagaaa a 51 <210> 184 <211> 51 <212> DNA <213> Bos taurus <400> 184 ttcccttttg cagtattttg gttttgaatt tctcttctta gaagtttggg t 51 <210> 185 <211> 51 <212> DNA <213> Bos taurus <400> 185 gatttgtgct gggcaagaag taaccgtctc tggcaagacc tggcatctgg g 51 <210> 186 <211> 51 <212> DNA <213> Bos taurus <400> 186 ggcctcacac agagaggggc ttggaacctt tgcactgcac ccaggaagac a 51 <210> 187 <211> 51 <212> DNA <213> Bos taurus <400> 187 aagggaaagt ttatgagaaa agacatagct gtgaggaagc agtcagatcc c 51 <210> 188 <211> 51 <212> DNA <213> Bos taurus <400> 188 aattaattca cattctgagg ttctgagcat taggacttct tcatgccccc t 51 <210> 189 <211> 51 <212> DNA <213> Bos taurus <400> 189 tggagttcag tgcccctaaa attataggga aattgccata tgtgatgtcg g 51 <210> 190 <211> 51 <212> DNA <213> Bos taurus <400> 190 gttcctagtg attccaacac actttatttt cttaattaca tcattttagt a 51 <210> 191 <211> 51 <212> DNA <213> Bos taurus <400> 191 ggttgatcag acttgggtca catgtatacc cttagaataa atttcaccag a 51 <210> 192 <211> 51 <212> DNA <213> Bos taurus <400> 192 tataggatat acctaggaat ggaactgata gatcacagga tccatgatga g 51 <210> 193 <211> 51 <212> DNA <213> Bos taurus <400> 193 taattttaaa catgttcaaa agtggggcga atggtgtaac atattctcat g 51 <210> 194 <211> 51 <212> DNA <213> Bos taurus <400> 194 gagcctccaa ctcatttatt tatctatctt cccaacagaa aaagcagcgt c 51 <210> 195 <211> 51 <212> DNA <213> Bos taurus <400> 195 gtatgtaatt gtatacagtg ctaaagtctc attttagggc gagggaaaaa t 51 <210> 196 <211> 51 <212> DNA <213> Bos taurus <400> 196 cttccaaatt tacctccatg tccattgcag gaagaaattg taaggacagt g 51 <210> 197 <211> 51 <212> DNA <213> Bos taurus <400> 197 taacacaaaa aatatgcaac cttccattta caatcaaatg ctaggtgtca t 51 <210> 198 <211> 51 <212> DNA <213> Bos taurus <400> 198 atatttgttt ctgatggttt atcttcacca gaggcatctc aaaccatcta c 51 <210> 199 <211> 51 <212> DNA <213> Bos taurus <400> 199 gggtacttct gagagcatgt gggagtgggg tctgtcctga aaaggcctca c 51 <210> 200 <211> 51 <212> DNA <213> Bos taurus <400> 200 cgacacttcc tgctccctca tctgcatgcc caagctgtca gcacgtctgc c 51 <210> 201 <211> 51 <212> DNA <213> Bos taurus <400> 201 tgtttccaac acctcttttc ctccccaaac cactgttcca aggcacatat t 51 <210> 202 <211> 51 <212> DNA <213> Bos taurus <400> 202 gaaatacaga aatgagctgc atagtgtcct gatgaattat atgcattcca g 51 <210> 203 <211> 51 <212> DNA <213> Bos taurus <400> 203 tttaatgatg aactcaatgt tactagagac agagagctgt tcaaagtttt t 51 <210> 204 <211> 51 <212> DNA <213> Bos taurus <400> 204 ttggaaagtc aaagaaataa actgagtact ttgggctgat ggaagtatgt t 51 <210> 205 <211> 51 <212> DNA <213> Bos taurus <400> 205 tctagacact gcaaagctac acttgagagt gtgtagtaca gtcactattt a 51 <206> 206 <211> 51 <212> DNA <213> Bos taurus <400> 206 ctgtatggag aaaatcctaa agacacggaa aactactgga gcttgttaat t 51 <210> 207 <211> 51 <212> DNA <213> Bos taurus <400> 207 tatcagtcac aacagcagaa aggaaacaca gtgaagtaca tatcatctct t 51 <210> 208 <211> 51 <212> DNA <213> Bos taurus <400> 208 cctgttatct ctgacttagt aacacgttgc tcagagcttg tcacgagatt t 51 <210> 209 <211> 51 <212> DNA <213> Bos taurus <400> 209 ctctctttgt ctctctggct ttccctctca ttagtctgtt taacgagctg g 51 <210> 210 <211> 51 <212> DNA <213> Bos taurus <400> 210 cccatttcct gcagtttcct ctcccaccgt ctagctgctc cgagagccgg t 51 <210> 211 <211> 51 <212> DNA <213> Bos taurus <400> 211 cccatggaag agcttgactt cctctctccc ccagccttga ccctgagcca c 51 <210> 212 <211> 51 <212> DNA <213> Bos taurus <400> 212 gtcccagttg gtggcacatg ggcagctgtt attttcaaga agctttctta g 51 <210> 213 <211> 51 <212> DNA <213> Bos taurus <400> 213 cattctgctt ggtggacaca ttgatgtact aagcagatct caggccctga g 51 <210> 214 <211> 51 <212> DNA <213> Bos taurus <400> 214 gcaagacatg ccgcctcaat gagcctcatt tcatggggac aatcaaaata a 51 <210> 215 <211> 51 <212> DNA <213> Bos taurus <400> 215 agagcccagt atcagataaa tacttttagg gtgaagcaat gaatgatcta a 51 <210> 216 <211> 51 <212> DNA <213> Bos taurus <400> 216 gaatatctta atgaaatcaa gcctccttaa gtgacctata aattcaatat g 51 <210> 217 <211> 51 <212> DNA <213> Bos taurus <400> 217 ttgacatata ccttctttca ggaactgaat catgaagagt ttaatcaagt a 51 <210> 218 <211> 51 <212> DNA <213> Bos taurus <400> 218 gcttgtaatg tagaaatctc cccccagtat aagaaggcta ttcagaagct g 51 <210> 219 <211> 51 <212> DNA <213> Bos taurus <400> 219 tcttttttta cttatatgac atgatagatg ttcactaatt gcctggagaa a 51 <210> 220 <211> 51 <212> DNA <213> Bos taurus <400> 220 ggattctgtt tcccagggat ccccaatgct catcacggac ttgttctggg t 51 <210> 221 <211> 51 <212> DNA <213> Bos taurus <400> 221 tgcctgtagc tatgataatg atgtaatttt tgagtattaa taagctgatg a 51 <210> 222 <211> 51 <212> DNA <213> Bos taurus <400> 222 catttcctaa aagcctctca tttggggaat ttcttcttta tcaaagtcca g 51 <210> 223 <211> 51 <212> DNA <213> Bos taurus <400> 223 ggccgtatcc atgtagcgag ggtgaccata tatttttttc caaactacgt c 51 <210> 224 <211> 51 <212> DNA <213> Bos taurus <400> 224 agcatctact ctaatcaaac ataattggat tgtgtatgac ttaaaagaat g 51 <210> 225 <211> 51 <212> DNA <213> Bos taurus <400> 225 agtcatacac caatcttggt gagaaagagc cagtttctct tattattaat a 51 <210> 226 <211> 51 <212> DNA <213> Bos taurus <400> 226 ttgtttctta ggtgcttttt ataagattta acttcatatt cagtaagcta a 51 <210> 227 <211> 51 <212> DNA <213> Bos taurus <400> 227 atggatggga cctggccagg cattactatt acgtagagag atgcccttgg g 51 <210> 228 <211> 51 <212> DNA <213> Bos taurus <400> 228 ggagaaaccc cttcatcagt agactcagcc agttagctta tactgcggtc c 51 <210> 229 <211> 51 <212> DNA <213> Bos taurus <400> 229 gggagaaaaa ggtgccggtt gtccatggag aggacagaat ggttcaggca a 51 <210> 230 <211> 51 <212> DNA <213> Bos taurus <400> 230 ttgacggcct ttctgatatt tgtctactcc taaatggttg aatgagtaca a 51 <210> 231 <211> 51 <212> DNA <213> Bos taurus <400> 231 tagaaggggt tgaaatcttg aattcagttc aagtttttga ttccacctcc t 51 <210> 232 <211> 51 <212> DNA <213> Bos taurus <400> 232 aacacatgat ggattggtta attggatcaa gcatgtgaca gtccagggta g 51 <210> 233 <211> 51 <212> DNA <213> Bos taurus <400> 233 atacagtcct cacttctgtc ctgaacgtgt ttggagacaa gcctctctta t 51 <210> 234 <211> 51 <212> DNA <213> Bos taurus <400> 234 cacacacaaa tgtaatgctg atgaatgagc atctgaaatg ttgggaatgg a 51 <210> 235 <211> 51 <212> DNA <213> Bos taurus <400> 235 tgaaaaaact ttgaaggaag gagcagaaag attggcaatt gccattaggc t 51 <210> 236 <211> 51 <212> DNA <213> Bos taurus <400> 236 ccaataaaat tttaaactgc accttctggg tgaaggaccc agaagtccta a 51 <210> 237 <211> 51 <212> DNA <213> Bos taurus <400> 237 cggtagagtg cagtttaaag aaatatagat ttcacaaaac ctacaatttg t 51 <210> 238 <211> 51 <212> DNA <213> Bos taurus <400> 238 agacatattc cttccacgat cgtgcttctc ttcttctgtt ctgagggccc a 51 <210> 239 <211> 51 <212> DNA <213> Bos taurus <400> 239 gttctaacat gcagtgtggt gactaaataa aagtattgaa ctggacactt g 51 <210> 240 <211> 51 <212> DNA <213> Bos taurus <400> 240 ggaggaaagg acaaagaatt aaatgggagg ttttcacatt gtcctgaaag c 51 <210> 241 <211> 51 <212> DNA <213> Bos taurus <400> 241 agggatcact cacatgacta tagttctctg gtgactcagt aaaggatgat c 51 <210> 242 <211> 51 <212> DNA <213> Bos taurus <400> 242 cctagtcact catccattcc tcagaatggc tcctttataa tcctcaaaac t 51 <210> 243 <211> 51 <212> DNA <213> Bos taurus <400> 243 acttatgctt tttaagccta atctctccct atcttttctt cagagcccat t 51 <210> 244 <211> 51 <212> DNA <213> Bos taurus <400> 244 ttcccattct gcttctctca cctctcacat tggctatctt tattataagg t 51 <210> 245 <211> 51 <212> DNA <213> Bos taurus <400> 245 ggagagataa aaggaccagc gaccaacaga cactgagaac cattattgca a 51 <210> 246 <211> 51 <212> DNA <213> Bos taurus <400> 246 aagaatacaa ttctcaacaa actggacata aagaaaacaa acttcattaa a 51 <210> 247 <211> 51 <212> DNA <213> Bos taurus <400> 247 ctaattttca tggtttcatc ttttacgtat tcaaacattt catatgatta t 51 <210> 248 <211> 51 <212> DNA <213> Bos taurus <400> 248 agatgctgct agagttgatg cagaaggtaa gtttccttcc tgttaagcaa c 51 <210> 249 <211> 51 <212> DNA <213> Bos taurus <400> 249 tatgtcagac tccatcttct gtgagatctg gctctcccca gagagtactg g 51 <210> 250 <211> 51 <212> DNA <213> Bos taurus <400> 250 ctttttggag tacacagaga tgctacgttc ttcccaaatt gatacataga a 51 <210> 251 <211> 51 <212> DNA <213> Bos taurus <400> 251 cagtcaaccc aactgtgagg aaaatttcag aaattaaact agactaagtt a 51 <210> 252 <211> 51 <212> DNA <213> Bos taurus <400> 252 actgcagagc aaacactgct tgtgagagga gccctgcatt ggcagaaatg g 51 <210> 253 <211> 51 <212> DNA <213> Bos taurus <400> 253 acctaccact ttctaactgc atggccttga gcaagttttt taaaaccaca c 51 <210> 254 <211> 51 <212> DNA <213> Bos taurus <400> 254 ctttttggga ctcaaataac tctgacccaa aacgttttaa taaattaaac a 51 <210> 255 <211> 51 <212> DNA <213> Bos taurus <400> 255 ccagtatgca tctctgaatg gggccatgtg taaccccttg tggtactgaa t 51 <210> 256 <211> 51 <212> DNA <213> Bos taurus <400> 256 cagttagatc agaatctcaa agtgacgcct aggaatcagt atttttaaat t 51 <210> 257 <211> 51 <212> DNA <213> Bos taurus <400> 257 tctggtacag agaattttgt tttccacatg gaaaaattca cgcctcagtt t 51 <210> 258 <211> 51 <212> DNA <213> Bos taurus <400> 258 ctatgatgac tggtctccct gggaacgtac agcttagcta ctccatccga a 51 <210> 259 <211> 51 <212> DNA <213> Bos taurus <400> 259 ataaatagaa ctgaatttgt tttaggttgt ctaatttctc taccattaca g 51 <210> 260 <211> 51 <212> DNA <213> Bos taurus <400> 260 agcaataaaa tacaccagac acggtgcact aacaacttgg ataaatcttg a 51 <210> 261 <211> 51 <212> DNA <213> Bos taurus <400> 261 agaacaatgt cacacccata tatgtatgca tgtatatgat tgcagacagg g 51 <210> 262 <211> 51 <212> DNA <213> Bos taurus <400> 262 aaataatttt aatcattttt attacacttc atggaatctt taaatgtggt c 51 <210> 263 <211> 51 <212> DNA <213> Bos taurus <400> 263 aagatttaaa taagaagttt gttcatttat tgagcaatga tttatcgaat g 51 <210> 264 <211> 51 <212> DNA <213> Bos taurus <400> 264 tggctttggc acttgaaatg tgtcttgtct taactgagat gtgctataaa t 51 <210> 265 <211> 51 <212> DNA <213> Bos taurus <400> 265 catagagatc atgtgctcat ctgttgcaaa tggcctgata tttcctactg t 51 <210> 266 <211> 51 <212> DNA <213> Bos taurus <400> 266 cccatccctt tccatcatcg ccctcgttct tggattggtc caatgcacag a 51 <210> 267 <211> 51 <212> DNA <213> Bos taurus <400> 267 gtatcatgct tgtttgcagg tacttttttt taatactctg agttgtgttt c 51 <210> 268 <211> 51 <212> DNA <213> Bos taurus <400> 268 taggccattt tcagatactg cagtagcagg aattatgatt cgtctatcca t 51 <210> 269 <211> 51 <212> DNA <213> Bos taurus <400> 269 cacttttaat catctaattg caactgatca ggaccctaca ggaccttccc a 51 <210> 270 <211> 51 <212> DNA <213> Bos taurus <400> 270 ctgtggctag aatgaggaaa tatgtttcat actgatacat atcatacaga t 51 <210> 271 <211> 51 <212> DNA <213> Bos taurus <400> 271 ccaggtcctc acagtggaga accagggaag actcccttgc ggtcctggca c 51 <210> 272 <211> 51 <212> DNA <213> Bos taurus <400> 272 ggtacatgat tactaaagga tcttacaaca aataatcaga cctcatttag t 51 <210> 273 <211> 51 <212> DNA <213> Bos taurus <400> 273 tggatagaag gatggaagca tgcacgcata gatggataca tgcaaggatg g 51 <210> 274 <211> 51 <212> DNA <213> Bos taurus <400> 274 tcactgttta taagtgttaa gcacagaaaa taagattgag acaaatttta a 51 <210> 275 <211> 51 <212> DNA <213> Bos taurus <400> 275 cttcagactt ccatagtttc ttaaccagaa gtcagtgtat gttcttattt t 51 <210> 276 <211> 51 <212> DNA <213> Bos taurus <400> 276 tataaccagt tgtcagcaaa acctctgacc taaaggacat cgagtggata g 51 <210> 277 <211> 51 <212> DNA <213> Bos taurus <400> 277 cttaaccact agtgccgtca ccgagcgctg gattatagag ttggcctcag c 51 <210> 278 <211> 51 <212> DNA <213> Bos taurus <400> 278 tgggttctgc cctggccttc aggagtacct gctctaggat cacccagagg t 51 <210> 279 <211> 51 <212> DNA <213> Bos taurus <400> 279 tgtggacact cttgccctct gtacatacac ccacatacat gcatccgtgc t 51 <210> 280 <211> 51 <212> DNA <213> Bos taurus <400> 280 acacgctttc tgatccttga atccattttt cattaactgt tcacattaag c 51 <210> 281 <211> 51 <212> DNA <213> Bos taurus <400> 281 aaaaactgca caacaaaaac ttagctactc gaggctcctg ctggctgtat c 51 <210> 282 <211> 51 <212> DNA <213> Bos taurus <400> 282 ctcagcgcaa cagaataaat gcccacttct cttcaaaatg taactgaggt t 51 <210> 283 <211> 51 <212> DNA <213> Bos taurus <400> 283 tgttagacta gtgtagagcc tcggaagcgt ccggtttcac accattattt t 51 <210> 284 <211> 51 <212> DNA <213> Bos taurus <400> 284 ccacagtgag aagcagtgca ctgcagtgga gagtagccca atcactccgc a 51 <210> 285 <211> 51 <212> DNA <213> Bos taurus <400> 285 gttttcagct gccctaagaa aggcatgctc aggtaatgac atcgtggaca t 51 <210> 286 <211> 51 <212> DNA <213> Bos taurus <400> 286 aaaggatccc ggaggtcctg ggtggttttc actccctata agtctgttgt c 51 <210> 287 <211> 51 <212> DNA <213> Bos taurus <400> 287 tatacccatt aacaggctac cagaacggca ggagttgagt gagcaaggac a 51 <210> 288 <211> 51 <212> DNA <213> Bos taurus <400> 288 aagttatgtc tacttgtgcc ctctgacttt gctctaccta aaaggcacta a 51 <210> 289 <211> 51 <212> DNA <213> Bos taurus <400> 289 ccatagctgc ccttccacgg ttgggcgtga agggtgacca tggagagaac a 51 <210> 290 <211> 51 <212> DNA <213> Bos taurus <400> 290 tgttcagctt ccacatctaa caatgcctcc ctcagcccac ctcacaagag g 51 <210> 291 <211> 51 <212> DNA <213> Bos taurus <400> 291 ctctgcctct ggactgtgct tccttgcagc acctgtgccc aggccccatc a 51 <210> 292 <211> 51 <212> DNA <213> Bos taurus <400> 292 ccctaacaga ctcagcccct gtcccatgtc ctctgctggg tgcttctggg a 51 <210> 293 <211> 51 <212> DNA <213> Bos taurus <400> 293 tggaggttct gaaaagaagg cattcgtctg tcatctgtgc acacgctcca g 51 <210> 294 <211> 51 <212> DNA <213> Bos taurus <400> 294 ctctaaattc gcagcgtccg cacagatctg tgcgtgtgtt catcctccaa t 51 <210> 295 <211> 51 <212> DNA <213> Bos taurus <400> 295 ttcaggcctc ccaccttgca gaactgtgag aaatcaatga ttatatttta a 51 <210> 296 <211> 51 <212> DNA <213> Bos taurus <400> 296 catgatgagt gctgtcagac agaggtctga tggtgcagga atcccacttt t 51 <210> 297 <211> 51 <212> DNA <213> Bos taurus <400> 297 agatgtccac cagagcttgc gcactctcct ctgacacagg ggtgcagttc t 51 <210> 298 <211> 51 <212> DNA <213> Bos taurus <400> 298 ggattgtctt cttccatagc ggcgcgacca tgttaatgaa cttactcttt t 51 <210> 299 <211> 51 <212> DNA <213> Bos taurus <400> 299 tggaatccaa cgctctgatc tttgcgcagc tggtcacgct catcattttg g 51 <210> 300 <211> 51 <212> DNA <213> Bos taurus <400> 300 acggagatgg tcaggtgtgg gtccaaactg ccttagagct caggaatgtc t 51 <210> 301 <211> 51 <212> DNA <213> Bos taurus <400> 301 tcaagcccat gaaggtggat aggagacctg gaagcggcgt ggccaagatc c 51 <210> 302 <211> 51 <212> DNA <213> Bos taurus <400> 302 ttctgctagg ataacactta cctaactgag tcttatagca ggcccctgac a 51 <210> 303 <211> 51 <212> DNA <213> Bos taurus <400> 303 tataagcaag agcaggtttt gagatttgaa aggtagagag aggaccaggt g 51 <210> 304 <211> 51 <212> DNA <213> Bos taurus <400> 304 tggtgggagg cgggccagag cggtcaccat aggaaccaca taaccatcac g 51 <210> 305 <211> 51 <212> DNA <213> Bos taurus <400> 305 ttccacaggc gagctacatg cccacgtgca aaaatgagtt taaggaaggc t 51 <210> 306 <211> 51 <212> DNA <213> Bos taurus <400> 306 gaggccaaaa aaactgaagt gctttgttct cacagtatta aaattagtga c 51 <210> 307 <211> 51 <212> DNA <213> Bos taurus <400> 307 taggtacaca gtatcacaat tctctcattt gatttgcctc acgcaataac a 51 <210> 308 <211> 51 <212> DNA <213> Bos taurus <400> 308 taaaaatcca actagtgact gcagagaggg gcttcagata gaagccctga c 51 <210> 309 <211> 51 <212> DNA <213> Bos taurus <400> 309 ccctgaggcc cagtaccttc ctcccaaccc cgtggcacca gagtctcagc c 51 <210> 310 <211> 51 <212> DNA <213> Bos taurus <400> 310 gagggcctga gggacagcaa tgaagagtca aggctgcagg ggaagggagg t 51 <210> 311 <211> 51 <212> DNA <213> Bos taurus <400> 311 ctcaaccctc ctgcgccagg tcaggtctac ttgtccaaag tgccccctac c 51 <210> 312 <211> 51 <212> DNA <213> Bos taurus <400> 312 caccaaggac tgaccctgca gccaccgcaa acagcaggta tctttccttt a 51 <210> 313 <211> 51 <212> DNA <213> Bos taurus <400> 313 agcatatggc caggttctgg gatatcatgg ctgtaaatga gggcccattg t 51 <210> 314 <211> 51 <212> DNA <213> Bos taurus <400> 314 ctgaatgtga ctgggtgtca tcaggctccc tgcagcaggt attggaggta c 51 <210> 315 <211> 51 <212> DNA <213> Bos taurus <400> 315 aacactaagg acgcctcagc ctcaccgaaa ccccggaaaa tgtcagcaga g 51 <210> 316 <211> 51 <212> DNA <213> Bos taurus <400> 316 ctccacccac atggagaatc tgtctatgtg ctccagccac agttgctggt c 51 <210> 317 <211> 51 <212> DNA <213> Bos taurus <400> 317 ggcccccatg tatactctga gagccattcg ctcactagag cgtgtcagtt c 51 <210> 318 <211> 51 <212> DNA <213> Bos taurus <400> 318 tgtgcctcct ctatggctgt taccagctgt gacagtctcc ttggcttgat c 51 <210> 319 <211> 51 <212> DNA <213> Bos taurus <400> 319 cagctgctcg tccagatgtg attgcttttg ctagagtaga ataacaggtg c 51 <210> 320 <211> 51 <212> DNA <213> Bos taurus <400> 320 aataccaaac aatgagccca agaacactga gagaaaattc acgccacatg c 51 <210> 321 <211> 51 <212> DNA <213> Bos taurus <400> 321 tacaagttct tagatttcaa gtccatcagt gatagtccag ccttgctcta t 51 <210> 322 <211> 51 <212> DNA <213> Bos taurus <400> 322 ttcatcttca cagcaacctc tgctacgggc tctacttcta atcctgtgtt t 51 <210> 323 <211> 51 <212> DNA <213> Bos taurus <400> 323 agtgggcctt ccagaccttt cctcctttgc tacccttgtt actgaagctc t 51 <210> 324 <211> 51 <212> DNA <213> Bos taurus <400> 324 gaatcacgtg gctattctgg cattactgag tctacattct gcaccgtgtg t 51 <210> 325 <211> 51 <212> DNA <213> Bos taurus <400> 325 actcacccac agatttggga ccctctcttc attgaggaca gaaggccaaa g 51 <210> 326 <211> 51 <212> DNA <213> Bos taurus <400> 326 caggccagcc cctcaagggt ctgggtgcag cagaggggac agggatgtcc t 51 <210> 327 <211> 51 <212> DNA <213> Bos taurus <400> 327 gctgattggt cccaggtgca actgagtcta acctgagctt tctaggaaga t 51 <210> 328 <211> 51 <212> DNA <213> Bos taurus <400> 328 aattcaggaa atcttcacta aggaacgtcc atgtggatga tcccaggagt a 51 <210> 329 <211> 51 <212> DNA <213> Bos taurus <400> 329 gttctataca tgggctttaa catattccct tctctttttc atattctcga t 51 <210> 330 <211> 51 <212> DNA <213> Bos taurus <400> 330 gaatgaatag gactatccac tgactctcca ccgtccctcg gctcagaaac t 51 <210> 331 <211> 51 <212> DNA <213> Bos taurus <400> 331 agctaattgg gagtcaacat cattactctt ttggttccca actggcttgg t 51 <210> 332 <211> 51 <212> DNA <213> Bos taurus <400> 332 ggccctgagg acaggtattc ttggcggctg cagcatgagc acaagggcaa a 51 <210> 333 <211> 51 <212> DNA <213> Bos taurus <400> 333 tttttgttat ggatctcacg ggtggagagt gggcgccctg ctgatttctg g 51 <210> 334 <211> 51 <212> DNA <213> Bos taurus <400> 334 atttccttcc tagcacactg atcttcctgc tcagttgttt tccacatatt g 51 <210> 335 <211> 51 <212> DNA <213> Bos taurus <400> 335 tgattaattg ttccccaaag caaagtggat ttggatcata gacttatagc t 51 <210> 336 <211> 51 <212> DNA <213> Bos taurus <400> 336 tacgaaggct tttcctccca tcctataaga ctagtgaaaa cgtgaggagc a 51 <210> 337 <211> 51 <212> DNA <213> Bos taurus <400> 337 ggggccatag gttatgcttt atgatcaggt gaggtggtta cctggacatt c 51 <210> 338 <211> 51 <212> DNA <213> Bos taurus <400> 338 gtttaatttt tatcttaatt cttctttggg gctctctgaa tttactagat c 51 <210> 339 <211> 51 <212> DNA <213> Bos taurus <400> 339 aggcgacact ccactcactg ccctcacaag cgtcggcaca gctgccaact g 51 <210> 340 <211> 51 <212> DNA <213> Bos taurus <400> 340 caagagtggg tgtgacttcc gcagctcctg cactcagtcc ttccaaggct c 51 <210> 341 <211> 51 <212> DNA <213> Bos taurus <400> 341 gcattgagtt aggcctcttc tttacgaaat tgctttgcat catctacagt g 51 <210> 342 <211> 51 <212> DNA <213> Bos taurus <400> 342 tgaaatattt atcaaatgaa taagcgatcg ttcccaagta tttccaggtg t 51 <210> 343 <211> 51 <212> DNA <213> Bos taurus <400> 343 actaaaacgc tgtattagcc agccttctta gctgggaggc acagccatgt g 51 <210> 344 <211> 51 <212> DNA <213> Bos taurus <400> 344 ggagtgtccc cctgaattac ctcaacagac ccctgcgaca gctctatgga c 51 <210> 345 <211> 51 <212> DNA <213> Bos taurus <400> 345 gcccacaaaa gaaaccccct acccactggc aatcactccc tattcctctc t 51 <210> 346 <211> 51 <212> DNA <213> Bos taurus <400> 346 gtgaggagta ggatttcagt ggacatgttt gtggacatca agatctccac t 51 <210> 347 <211> 51 <212> DNA <213> Bos taurus <400> 347 ggagactcca ggaagtgcct gccccgtcca gtccctcaca agcccctcaa c 51 <210> 348 <211> 51 <212> DNA <213> Bos taurus <400> 348 aacttccctg gttgagagat cagaaaggga agctgtcatg ctctgtgaag a 51 <210> 349 <211> 51 <212> DNA <213> Bos taurus <400> 349 tcctggtttt tccttctggg ctgaacatcc tcaccctgct ggctcaggtc c 51 <210> 350 <211> 51 <212> DNA <213> Bos taurus <400> 350 aaactacagc tcccagcttc tggctgccat tctagctcca catggccaca g 51 <210> 351 <211> 51 <212> DNA <213> Bos taurus <400> 351 acctcatata gcccaactgt tagttcaaaa ctaacagtgt aaggactccc t 51 <210> 352 <211> 51 <212> DNA <213> Bos taurus <352> 352 ccagaacaag cctctcagct gtagcgagcc tccttcagcc tggagtgaga g 51 <210> 353 <211> 51 <212> DNA <213> Bos taurus <400> 353 attccattct atagtgtaga agcactgcca tattggccac caggatcatg t 51 <210> 354 <211> 51 <212> DNA <213> Bos taurus <400> 354 tgctggagtg aaggacggat atgctctgtg cccactgggc tcacggctaa c 51 <210> 355 <211> 51 <212> DNA <213> Bos taurus <400> 355 gttgaaccaa agaggatatt aaagatagca gggcaatggc tccctactgc c 51 <210> 356 <211> 51 <212> DNA <213> Bos taurus <400> 356 aaaagaaaag actgaagaaa tgagaaaaga attcctggta acatgtaagt c 51 <210> 357 <211> 51 <212> DNA <213> Bos taurus <400> 357 ttcaggaccg actgcctgat ggacagacag acagagggat gattgcagca t 51 <210> 358 <211> 51 <212> DNA <213> Bos taurus <400> 358 caccaagcct atacatcttc cttttaatac tgatcactag tatcccccta g 51 <210> 359 <211> 51 <212> DNA <213> Bos taurus <400> 359 gcccagcact catctctcag tatttggctt cttgagacat gagcagccaa g 51 <210> 360 <211> 51 <212> DNA <213> Bos taurus <400> 360 aagatcccca aatcccagga gtacctatgg cacacattcc agagctccct g 51 <210> 361 <211> 51 <212> DNA <213> Bos taurus <400> 361 catggtgcta gctatcctgc attagcatct ccggtgctca caagggcttc c 51 <210> 362 <211> 51 <212> DNA <213> Bos taurus <400> 362 atagttacca tctagtttta atatagtctt actccatagc ctgacatttt a 51 <210> 363 <211> 51 <212> DNA <213> Bos taurus <400> 363 ggagaaaact ccagccaccc aaggggcttc actaacgagc tccactcaat a 51 <210> 364 <211> 51 <212> DNA <213> Bos taurus <400> 364 aaccataggt aattcaaggc cttgcctggc aggcctcatg aggagaaact g 51 <210> 365 <211> 51 <212> DNA <213> Bos taurus <400> 365 ggctcaacca gtgtgcacca caaccgtgct gttaaatgca gaaatactgt c 51 <210> 366 <211> 51 <212> DNA <213> Bos taurus <400> 366 gagggccact ggcagagtgg gggaattgag gctgacaaag atggcaggaa g 51 <210> 367 <211> 51 <212> DNA <213> Bos taurus <400> 367 gttgggattt ccaaaacctg tgagctccaa ctggacactt tgtatggcac g 51 <210> 368 <211> 51 <212> DNA <213> Bos taurus <400> 368 tcctaggctg tcagctggca ggcctgtgca gacttggctt gtgtccttgt g 51 <210> 369 <211> 51 <212> DNA <213> Bos taurus <400> 369 tcagagctgc ttctgacatg tctttgtact agagaatcaa ttgaccctaa g 51 <210> 370 <211> 51 <212> DNA <213> Bos taurus <400> 370 gttttgacta tgttgtctgc ttcctgtgtt caacttctac tgatgtacgc t 51 <210> 371 <211> 51 <212> DNA <213> Bos taurus <400> 371 tttagagtaa ctggcaggca tcagcacaat ttctcttaat acttcagcat a 51 <210> 372 <211> 51 <212> DNA <213> Bos taurus <400> 372 ccataatttg gtcatggatc agaaacgatg aaaagagaca tacttgaaat t 51 <210> 373 <211> 51 <212> DNA <213> Bos taurus <400> 373 cctcttaacc cctacctttg tcttatgaga tcttacacag tctttatgct a 51 <210> 374 <211> 51 <212> DNA <213> Bos taurus <400> 374 cggtatagac agtaagatcc tttctcgagc agtgccttgc ggcagagtaa a 51 <210> 375 <211> 51 <212> DNA <213> Bos taurus <400> 375 gttgcaacag cttctttact atcttatctt atttctttgc cataatagtc t 51 <210> 376 <211> 51 <212> DNA <213> Bos taurus <400> 376 cagaaactcc cctttagagc tctgagcagc agggggaggc tggtctttgg g 51 <210> 377 <211> 51 <212> DNA <213> Bos taurus <400> 377 tcctgatcaa tttccatctc tctaaatact cacatgttca atgaacacaa c 51 <210> 378 <211> 51 <212> DNA <213> Bos taurus <400> 378 tagctaattg tgtcaaattt gaagatgctg tactccgcgt cctctccaca c 51 <210> 379 <211> 51 <212> DNA <213> Bos taurus <400> 379 tgggagatgg tctaggaaaa agaagcatct gcacctctgt taccgcgaga c 51 <210> 380 <211> 51 <212> DNA <213> Bos taurus <400> 380 gcctgaccaa cggcaacctg acccctacta taacttctgt ctctactctc c 51 <210> 381 <211> 51 <212> DNA <213> Bos taurus <400> 381 gcagagctga ggcatgggaa gctgaggcat gaggagactg agcctcatgt c 51 <210> 382 <211> 51 <212> DNA <213> Bos taurus <400> 382 gaatttataa ggtggatgtt ttcaggttca tgtattgctg agagtgagct a 51 <210> 383 <211> 51 <212> DNA <213> Bos taurus <400> 383 acgaggccat gggccgcgag gtgacagcgc tcaagagcaa gctcaggtga g 51 <210> 384 <211> 51 <212> DNA <213> Bos taurus <400> 384 agagcttaga aaaagttaac tactgttctt gttacatttt acaaatacat t 51  

Claims (8)

한우 개체인식 또는 혈통확인을 위한, 염기서열 26번째에서 G↔A, C↔T, G↔T, C↔A, T↔A 및 C↔G로 구성된 군에서 선택된 어느 하나의 단일염기변이를 갖는 서열번호 1 내지 384로 구성된 SNP(single nucleotide polymorphism) 유전자 세트.It has a single base mutation selected from the group consisting of G↔A, C↔T, G↔T, C↔A, T↔A and C↔G in the 26th base for the identification or pedigree of Hanwoo. Single nucleotide polymorphism (SNP) gene set consisting of SEQ ID NOs: 1 to 384. 제 1항에 있어서, The method of claim 1, 상기 SNP 유전자 세트는 마이너 대립유전자 빈도(minor allele frequency; MAF)가 한우에서 0.3 이상인 것을 특징으로 하는 SNP 유전자 세트.The SNP gene set has a minor allele frequency (MAF) of SNP gene set, characterized in that more than 0.3 in Hanwoo. 삭제delete 삭제delete 제 1항 또는 제 2항의 SNP 유전자 세트를 포함하는 한우 개체인식 또는 혈통확인을 위한 키트.Claim 1 or 2 kit for identification or pedigree of Hanwoo comprising the SNP gene set. 염기서열 26번째에서 G↔A, C↔T, G↔T, C↔A, T↔A 및 C↔G로 구성된 군에서 선택된 어느 하나의 단일염기변이를 갖는 서열번호 1 내지 384로 구성된 SNP(single nucleotide polymorphism) 유전자 세트를 사용한 한우 개체인식 방법.SNP consisting of SEQ ID NOs: 1 to 384 having any single base mutation selected from the group consisting of G↔A, C↔T, G↔T, C↔A, T↔A, and C↔G Single nucleotide polymorphism) Hanwoo individual recognition method using gene set. 염기서열 26번째에서 G↔A, C↔T, G↔T, C↔A, T↔A 및 C↔G로 구성된 군에서 선택된 어느 하나의 단일염기변이를 갖는 서열번호 1 내지 384로 구성된 SNP(single nucleotide polymorphism) 유전자 세트를 사용한 한우 혈통확인 방법.SNP consisting of SEQ ID NOs: 1 to 384 having any single base mutation selected from the group consisting of G↔A, C↔T, G↔T, C↔A, T↔A, and C↔G Hanwoo cattle pedigree method using single nucleotide polymorphism gene set. 염기서열 26번째에서 G↔A, C↔T, G↔T, C↔A, T↔A 및 C↔G로 구성된 군에서 선택된 어느 하나의 단일염기변이를 갖는 서열번호 1 내지 384로 구성된 SNP(single nucleotide polymorphism) 유전자 세트를 사용한 한우 원산지 판별 방법.SNP consisting of SEQ ID NOs: 1 to 384 having any single base mutation selected from the group consisting of G↔A, C↔T, G↔T, C↔A, T↔A, and C↔G Single nucleotide polymorphism) A method for determining the origin of Korean cattle using a set of genes.
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