KR100947643B1 - Composition comprising the distillate of rice husk as an active ingredient for preventing and treating allergy - Google Patents

Abstract

PURPOSE: A composition containing distilled liquid of rice husk as an active ingredient is provided to reduce total weight of the epidermis, dermis and whole skin. CONSTITUTION: A pharmaceutical composition for preventing and treating contact dermatitis or atopic dermatitis contains distilled liquid of rice husk as an active ingredient. A method for producing the distilled liquid of the rice husk comprises: a first step of treating rice husk at 300°C-600°C for 40 to 120 minutes; a second step of condensing steam discharged during hydrocarbon processing to obtain crude distilled liquid; and a third step of distilling the crude distilled liquid at 40°C ~ 70°C.

Description

Composition comprising the distillate of rice husk as an active ingredient for preventing and treating allergy

The present invention relates to a pharmaceutical composition for the prevention and treatment of allergic diseases and health functional foods containing chaff distillate as an active ingredient.

[Document 1] Choi, Byung-Hwi et al. 4, Allergy, 4 , pp2-48, 2001

[2] Wuthrich B., Int . Arch . Allergy Appl . immunol ., 90 , pp 3-10, 1989

[Reference 3] Miller JS. et al., Curr . Opin . Immunol . , 1 , pp637-642, 1989

4 Nakagawa et al., J. Invest . Dermatol . , 57, pp 369-377, 1971

Oka et al., Dermatologica ., 172, pp 12-17, 1986.

[6] Friedmann, Adv . Dermatol ., 5, pp 175-195, 1990

[7] Upadhye and Maibach, Contact Dermatitis . , 27, pp281-286, 1992

8 Bronaugh et al., Food Chem . Toxicol ., 32, pp113-117, 1994

9 Kolde, Exp . Dermatol ., 3 , pp 269-275, 1994

10. Jet et al., Vet . Pathol ., 36 , pp 42-50, 1999

[Document 11] Korean Institute of Health Process, 2004

[Document 12] Kang Dae Gap, New Functional Natural Product Material Exploration and Utilization Technology, Development of Ceramic Raw Materials and Industrial Materials Using Silicon Sieve of Rice, Korea Atomic Energy Research Institute

Document 13 Pokharel et al., Evid Based Complement Alternat Med ., 5 , pp 173-180, 2008

[14] Yamazaki et al., Ind . Health ., 43 , pp308-319, 2005

15. Arts et al., Food Chem . Toxicol ., 34, pp 55-62, 1996

[16] Lee et al., J. Toxicol . Pub . Health ., 22 , pp 117-126, 2006

17. Lee et al., Lab . Anim . Res ., 521 , pp 299-305, 2005

18 Shiohara et al., J. Dermatol . Sci ., 36, pp 1-9, 2004

Allergy refers to a hypersensitivity reaction caused by contact with a harmless antigen, an allergen that is innate or acquired and impaired in immune function, such as anaphylaxis. there are, allergic rhinitis (allergic rhinitis), asthma (asthma), atopic dermatitis (atopic dermatitis), insect allergies, food allergies, drug allergies and hives (urticaria), etc. (4 choebyeonghwi others, allergies, 4, pp2-48 , 2001; Wuthrich B., Int. Arch. Allergy Appl. Immunol ., 90 , pp 3-10, 1989).

Allergy is involved in the interaction of various factors, such as genetic predisposition, environmental factors, pharmacological abnormalities, immunological factors, etc. The hypersensitivity reaction of the immune function of the living body is 4 It is divided into the types of branches. Type I hypersensitivity reactions occur when IgE (immunoglobulin E), which is excessively produced by repeated exposure of allergens, binds to mast cells, resulting in the release of histamine-containing granules. Anaphylactic shock or local itching and inflammation are typical diseases, including atopic dermatitis and asthma.

Type II hypersensitivity reactions are caused by Null (K) cells, which destroy cells as antibodies bind to normal cells such as red blood cells, white blood cells, and platelets, resulting in thrombocytopenia. Hemolytic anemia caused by cyclosporin ), Leukocyte reduction by aminopyrine (leukopenia), platelet reduction by quinidine (thrombocytopenia), and the like. Type III hypersensitivity is a disease in which polymorphonuclear (PMN) cells damage tissues by the deposition of antigen-antibody complexes in tissues, such as systemic lupus erythomatosis. On the other hand, type 4 hypersensitivity reactions are delayed type hypersensitivity reactions that cause sensitized T cells to differentiate into memory cells and subsequently cause dermatitis when resensitized, called contact dermatitis. In the sense, it belongs to the category of contact hypersensitivity (Miller JS. Et al., Curr . Opin . Immunol . , 1 , pp637-642, 1989).

Contact dermatitis is a dermatitis caused by contact with foreign substances and is a kind of eczema. Contact dermatitis has irritant contact dermatitis and allergic contact dermatitis depending on the mechanism in which it occurs, and one substance can cause these two reactions simultaneously.

Allergic contact dermatitis is a symptom of contact with the causative agent when allergic to a 'specific' substance, and when a sensitized reaction occurs upon contact with a particular substance, re-contacting the substance. Occasional dermatitis can be referred to as allergic contact dermatitis. This sensitization may occur with a single exposure, causing dermatitis. However, symptoms usually occur after prolonged exposure to the skin for several months or more. In humans, skin appears within 12 to 72 hours after re-exposure to the sensitized substance. Reaction. It is often difficult to find the causative agent.

The substances that can cause allergic contact dermatitis are various organic compounds, metals, lacquer and other plants, additives, cosmetics, fragrances, and synthetic resins contained in substances used around life. Among the most common allergens are metals, the most common cause of metal allergy is nickel, and allergic contact dermatitis caused by nickel is present in 7-10% of the population. In addition, allergic contact dermatitis caused by dyes or rubber is common, and allergic contact dermatitis caused by lacquer trees is also common.

Another irritant of contact dermatitis Contact allergic dermatitis, unlike allergic contact dermatitis, is a dermatitis caused by a substance that causes dermatitis in all cases when given a certain concentration of irritation. In other words, if stimulated with a certain concentration of stimulant may occur in all people. The stimulus level of the stimulant In large cases, even one or two touches may cause chemical burns, red spots, swelling, and blisters on the skin, and iterative and itchy skin if repeatedly irritated by weak irritants. Thickening. Therefore, the incidence is much higher than that of allergic contact dermatitis.

Causes of irritant contact dermatitis can be strong acids, strong alkalis, water detergents, chronically released oozes, diapers, and professionally used substances. The types of skin rashes caused by these causes include chemical burns caused by contact with strong acids or strong alkalis, housewives eczema, which is frequently caused by repeated long-term contact with water, detergents, soaps, etc. Irritant contact dermatitis includes diaper dermatitis caused by moisture and friction in the groin of children wearing diapers and occupational dermatitis caused by exposure to chemicals in various workplaces.

Contact dermatitis is classified into sun contact dermatitis, mercury contact dermatitis, or metal contact dermatitis depending on the contact material.

Plant-causing contact dermatitis includes warsi oil (extracted from mango), aloe, kiwi, celery and lemon, but it is most often caused by contact with lacquer after hiking in spring. Contact dermatitis caused by food is characterized by erythematous lesions accompanied by blisters at the contact site.

Metal contact dermatitis is often caused by the plating of watches, ornaments, etc. by elution by sweat, and is used as an antidote such as nickel chromium, a metal mixed with metal used for vulcanization of metal or rubber used as a material for textile dyes, or rubber. Organic mercury used, tameosal, kali dichromate used in leather operation, and the like.

DNCB (Dinitrochlorobenzene) is a representative substance that causes delayed contact dermatitis. It is known that repeated exposure of DNCB causes skin swelling and thickening of skin tissue due to infiltration of CD4 + and CD8 + T cell mediated inflammatory cells. Sexual dermatitis animal models are currently one of the most widely used animal models (Nakagawa et al., J. Invest . Dermatol . , 57, pp 369-377, 1971; Oka et al., Dermatologica ., 172, pp 12-17, 1986; Friedmann, Adv . Dermatol ., 5, pp 175-195, 1990; Upadhye and Maibach, Contact Dermatitis , 27, pp 281-286, 1992; Bronaugh et al., Food Chem . Toxicol ., 32, pp 113-117, 1994; Kolde, Exp . Dermatol ., 3 , pp 269-275, 1994). Thickening of skin tissue with histologically significant inflammatory cell infiltration is known (J et al., Vet . Pathol ., 36 , pp42-50, 1999).

When allergens are recognized by IgE, they are adhered to Langerhans cell surface IgE-attached Fc receptors, and T lymphocytes are activated by delivering antigens to T lymphocytes. At this time, auto peptide or staphylococcus T lymphocytes can also be activated by superantigens of aureus ). Unlike other skin diseases, inflammatory cells infiltrating skin lesions of atopic dermatitis are mainly Th2 cells, which produce cytokines such as IL-4 and IL-5, which promote blood IgE elevation and eosinophils, and cAMP force Abnormal monocytes of atopic dermatitis with elevated phosphodiesterase increase the production of prostaglandins (PGE), thereby inhibiting infiltration of Th1 lymphocytes. Th1 lymphocytes are also inhibited by the tumor necrosis factor (TNF) released from splenocytes that are readily activated in atopic dermatitis. Eventually it inhibits Th1 proliferation in atopic dermatitis and leads to a decrease in cell mediated immunity. Cytokines induce ectopic lesions by activating vascular endothelial cells to induce or increase the expression of various cell adhesion molecules, thereby facilitating the return of memory T lymphocytes (CHO Byung-Hwi et al. 4, allergy, 4 , pp2-48). , 2001).

In recent years, urban people are increasingly exposed to various allergens, such as mites living on carpets in their homes, dust that increases with humidity, pet hair, pollen, new fruits and food. As a result, asthma and atopic dermatitis, type 1 hypersensitivity, have increased in recent years and become a social problem, many researchers have been focused on the search for substances that can mitigate immune hypersensitivity from natural products.

In addition, the exploration of new functional substances from natural products is accelerating with the establishment of the Korea Food and Drug Administration's notification on the evaluation of the efficacy and safety of functional foods and functional cosmetics and the approval procedure (Korean Health Procedures Research Association, 2004). Reflecting these social phenomena, the development of more effective new drugs through the scientification of natural products in a highly industrialized society has been recognized as an important factor in securing national competitiveness. Various food sweeteners, flavorings, functional foods, functional cosmetics, natural pesticides, etc. have been developed in various ways, but the systematic research is still insufficient.

Rice husks are the husks of rice, the largest by-product of rice processing, accounting for 20% of the weight of rice. Domestic rice husk production is estimated at 5,961,693 tons of rice in 2007, and about 1.2 million tons of rice husk are produced annually, which is about 20%. Conventionally, it was produced in a small rice mill processing plant and limited in its utilization method, but now a large-scale milling plant is planned to be installed by 2010, and rice grain by-product chaff will be concentrated in the rice mill juksan complex. The efficient use of this rice hull is very important.

Chaff has the proper characteristics as an energy source, but is currently classified as a waste. Chaff is known to be inadequate for compost and livestock feed due to its low internal corrosion and low digestion efficiency because it is heavily coated with silicon. The main components of these rice hulls are 27-30% of soluble carbohydrates, 35-46% crude fiber, 13-21% crude ash, 2-3% crude protein, and 0.3-0.8% crude fat. It is known (Kang Dae Gap, new functional natural material search and utilization technology for agricultural products, development of ceramic raw materials and industrial materials using silicon bodies of rice, Korea Atomic Energy Research Institute).

As chaff has such characteristics, it is classified as a waste because it has no use and proper treatment in modern times, and it is one of the important things to make the chaff efficient and use the waste to make it valuable.

Therefore, the present inventors prepared the chaff herb distillate with this chaff as a material, and then, using this animal model of contact dermatitis allergic disease caused by DNCB, the main antibody of allergic reaction of IgE in affected tissues The present invention was completed by confirming the anti-allergic effect by inhibiting production and confirming that the total thickness of the dermis, epithelium and skin is reduced.

In order to achieve the above object, the present invention provides a pharmaceutical composition for the prevention and treatment of allergic diseases containing chaff herb distillate as an active ingredient.

The present invention also provides a health functional food for the prevention and improvement of allergic diseases containing chaff distillate as an active ingredient.

Rice husk distillate, as defined herein, is prepared using a carbonizer (DCH-600, Daewon GSI), and the chaff is 200 to 800 ° C, preferably 300 to 600 ° C, for 10 minutes to 200 minutes, Preferably, the first step of carbonizing for 40 minutes to 120 minutes; A second step of condensing the smoke discharged during the carbonization process to obtain crude chaff herb distillate; Obtaining the chaff distillate of the present invention through a process comprising a third step of distilling the crude crude chaff distillate of the second step at a low temperature of 20 ℃ ~ 90 ℃, preferably 40 ℃ ~ 70 ℃ can do.

The organic composition of the chaff distillate as defined herein is usually 20 to 80%, preferably 40 to 70%, acidic fraction 5 to 45%, preferably 15 to 35%, basic fraction of the phenolic fraction The 0.01 to 20%, preferably 0.5 to 10%, the neutral component fraction is in the range of 1 to 40%, preferably 10 to 30%, characterized in that it comprises the above organic material composition.

Allergic diseases as defined herein include hypersensitivity, allergic rhinitis, asthma, allergic conjunctivitis, allergic dermatitis, atopic dermatitis, contact dermatitis, urticaria, insect allergy, food allergy or drug allergy, preferably allergic rhinitis, asthma , Allergic dermatitis, atopic dermatitis, contact dermatitis, urticaria, food allergy or drug allergy, more preferably atopic dermatitis or contact dermatitis.

Hereinafter, the present invention will be described in detail.

Rice husk distillate of the present invention can be prepared as follows.

The rice hull distillate was prepared using a carbonizer (DCH-600, Daewon GSI), and the rice husk was 200 to 800 ° C, preferably 300 to 600 ° C, 10 to 200 minutes, preferably 40 A first step of carbonizing for minutes to 120 minutes; A second step of condensing the smoke discharged during the carbonization process to obtain crude chaff herb distillate; Obtaining the chaff distillate of the present invention through a process comprising a third step of distilling the crude crude chaff distillate of the second step at a low temperature of 20 ℃ ~ 90 ℃, preferably 40 ℃ ~ 70 ℃ can do.

The present invention provides a pharmaceutical composition for the prevention and treatment of allergic diseases containing the chaff herb distillate obtained by the above production method as an active ingredient.

In another aspect, the present invention provides a health functional food for the prevention and improvement of allergic diseases containing chaff herb distillate obtained by the above production method as an active ingredient.

The pharmaceutical composition for the prevention and treatment of allergic diseases containing chaff herb distillate of the present invention comprises 0.1 to 50% by weight of the chaff herb distillate based on the total weight of the composition.

However, the composition as described above is not necessarily limited thereto, and may vary according to the condition of the patient and the type and extent of the disease.

The pharmaceutical composition containing rice hull distillate of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.

Pharmaceutical compositions containing rice hull distillate according to the present invention, powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, oral formulations, external preparations, suppositories, and sterile injectable solutions, respectively, according to a conventional method. Formulated in the form of can be used. The carriers, excipients and diluents that may be included in the composition containing the chaff distillate of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch , Acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil Can be mentioned. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used. Solid form preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, which form at least one excipient such as starch, calcium carbonate, sucrose or lactose in the compound. Mixed with gelatin. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The amount of chaff distillate of the present invention may vary depending on the age, sex, and weight of the patient, but may be 0.1 to 100 mg / kg, preferably 1 to 10 mg / kg once or several times daily. The dosage may also be increased or decreased depending on the route of administration, the severity of the disease, sex, weight, age, and the like. Therefore, the above dosage does not limit the scope of the present invention in any aspect.

The pharmaceutical composition may be administered to various mammals such as mice, mice, livestock, humans, and the like. All modes of administration can be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or cerebrovascular injections.

The present invention provides a dietary supplement for the prevention and improvement of allergic diseases containing chaff distillate as an active ingredient. Foods to which it may be added include various foods, powders, granules, tablets, capsules, syrups, beverages, gums, teas, vitamin complexes, and health functional foods.

The chaff distillate of the present invention is a drug that can be used with confidence even when taken for a long time for the purpose of prevention because there is little toxicity and side effects.

Rice husk distillate of the present invention may be added to food or beverage for the purpose of preventing and improving allergic diseases. At this time, the amount of rice hull distillate in the food or beverage may be added to 0.01 to 15% by weight of the total food weight, the health functional beverage composition is based on 100ml in a ratio of 0.02 to 10g, preferably 0.3 to 1g Can be added.

The health functional beverage composition of the present invention is not particularly limited to other ingredients except for containing the chaff distillate as an essential ingredient in the indicated ratios, and various flavors or natural carbohydrates as additional ingredients as in the usual beverages. It may contain. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of said natural carbohydrates is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.

In addition to the above, chaff distillate of the present invention is a variety of nutrients, vitamins, minerals (electrolytes), synthetic flavors such as synthetic and natural flavors, coloring and neutralizing agents (such as cheese, chocolate), pectic acid and salts thereof, Alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the extracts of the present invention may contain pulp for the production of natural fruit juices and vegetable drinks. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected from the range of 0 to about 20 parts by weight per 100 parts by weight of rice hull distillate of the present invention.

As described above, rice hull distillate has the effect of inhibiting the production of IgE, a major antibody of allergic reactions, in the affected tissues of DNCB-induced contact dermatitis allergic disease animal models, As it shows a reducing effect on the dermis and the overall thickness of the skin, it can be usefully used as a pharmaceutical composition and health functional food for the prevention and treatment of allergic diseases.

Hereinafter, the present invention will be described in detail by the following reference examples, examples and experimental examples.

However, the following Examples, Reference Examples and Experimental Examples are merely illustrative of the present invention, but the content of the present invention is not limited thereto.

Example  One. Chaff  Preparation of Distillate

The rice husk distillate was obtained by direct carbonization using 400kg of rice husk provided by Sunsan Rice Grain Treatment Plant (RPC). A first step of carbonizing for minutes to 120 minutes; A second step of condensing the smoke discharged during the carbonization process to obtain crude bran herb distillate; The crude chaff herb distillate (80kg, 20%) of the second step under reduced pressure for purification, the chaff herb distillate of the present invention through a process comprising a third step of purifying at a low temperature of 40 ℃ ~ 70 ℃ (hereinafter 50 kg) was obtained.

Example  2. Chaff  Distillate ( DWC Sample preparation of (1)

A sample prepared by diluting rice husk distillate (DWC) to 1/10 concentration with 1 ml / 9 ml using saline solution is named “DWC-1” and diluted to 1/50 concentration with 1 ml / 49 ml. DWC-2 ”, and the sample made by diluting 1/100 concentration to 1ml / 99ml was named" DWC-3 "was used in the experiment. Saline was used as a control.

Example  3. Chaff  Distillate ( DWC Sample preparation (2); Of manufacturing example (CR)  Produce

Product cream containing rice hull distillate (DWC) was prepared and used in the experiment. Preparation Example 1 (hereinafter referred to as "CR-1") which is a cream containing no chaff distillate and Preparation Example-2 (hereinafter referred to as "CR-2") as a cream containing chaff distillate Experiment using.

3-1. CR Manufacture of -1

order Raw material Content (g) One Purified water Quantity 2 glycerin 2.0 parts by weight 3 1.3-butylene glycol 2.0 parts by weight 4 Caprylic / Capric Triglycerides 3.0 parts by weight 5 β-glucan 2.0 parts by weight 6 Dimethicone 1.5 parts by weight 7 lecithin 1.0 parts by weight 8 Squalane 20.0 parts by weight 9 Stearic acid 4.0 parts by weight 10 Cetanol 3.0 parts by weight 11 Antioxidant 1.0 parts by weight 12 Glyceryl Stearate 1.5 parts by weight 13 Triethanolamine 0.2 parts by weight 14 Paraoxybenzoic acid ester Quantity

3-2. CR Manufacture of -2

order Raw material Content (g) One Purified water Quantity 2 glycerin 2.0 parts by weight 3 1.3-butylene glycol 2.0 parts by weight 4 Caprylic / Capric Triglycerides 3.0 parts by weight 5 β-glucan 2.0 parts by weight 6 Dimethicone 1.5 parts by weight 7 lecithin 1.0 parts by weight 8 Squalane 20.0 parts by weight 9 Stearic acid 4.0 parts by weight 10 Cetanol 3.0 parts by weight 11 Antioxidant 1.0 parts by weight 12 Glyceryl Stearate 1.5 parts by weight 13 Triethanolamine 0.2 parts by weight 14 Rice husk distillate 0.5 parts by weight 15 Paraoxybenzoic acid ester Quantity

Example  4. Chaff  Analysis result of constituents in distillate

The rice husk distillate is obtained by carbonizing rice husk and obtaining the distillate. The rice husk distillate is made on the same principle as commercial wood vinegar prepared by carbonizing wood board.

Therefore, in order to analyze the components of the rice husk distillate (DWC) and commercial wood vinegar obtained in Example 1, the following experiment was performed.

Material analysis of each rice husk distillate (DWC) and commercial wood vinegar was carried out using GC (HP 5890 II, Hewlett-Packard (Calo Alto California USA, column (5% -Phenyl) Methylpolysiloxane coating, Korea Food Research Institute) .

Experimental results, as shown in the following table, the comparative analysis of the contents of the components identified in the rice husk distillate and commercial wood vinegar,

Eleven components were identified as acids, and volatile organic acids such as acetic acid, propionic acid and isobutyric acid were detected. Volatile organic acids are components that directly affect the pH of wood vinegar, and their contents and composition ratios vary greatly from sample to sample. In commercial wood vinegar, acetic acid content was higher than propionic acid content. However, rice hull distillate was characterized in that the content of propionic acid (propionic acid) higher than acetic acid (acetic acid). This difference is believed to be due to the difference in the materials used.

Twelve components were identified as aldehydes and ketones. Aldehydes and ketones were also generally contained in rice husk distillate rather than commercial wood vinegar, and rice hull distillate contained more of chaff herb obtained from direct carbonization than indirect carbonization.

13 kinds of furan and pyran are identified, and these are the components that are produced when heating saccharides or cellulose at high temperature, and they are widely used as food additives because they have a sweet smell, sugar and caramel smell. .

Phenols were identified as 11 components, and phenols were generally higher in rice husk distillate than in commercial wood vinegar, and higher in distillates obtained by direct carbonization than indirect carbonization.

Therefore, the experiment was carried out using the rice husk distillate obtained by direct charcoal chaff.

As a result of the analysis, comparing the rice husk distillate prepared by carbonizing rice husk with the wood vinegar prepared by carbonizing plain wood, the contents of each component were different even though the ingredients were similar. there was.

division manufacture company Raw material Condensate Yield (%) Remarks Rice husk distillate Daewon GSI Chaff 10.8 Direct Carbonization Condensate Chaff 7.5 Indirect Carbonized Condensate Throat A company kind of oak 2.52 Direct Carbonization Condensate Throat Company B kind of oak 1.90 Direct Carbonization Condensate Throat Company C Pine tree 2.70 Direct Carbonization Condensate

Compound (mg / kg) Rice husk distillate Wood vinegar Direct carbonization Indirect carbonization Direct carbonization Acetic acid 2067.4 1300.3 919.3 Propionic acid 2960.8 1852.8 271.3 iso-Butyric acid 1803.3 1031.9 764.1 n-Butyric acid 340.0 220.7 653.6 Benzoic acid 131.8 45.5 6.0 Cyclohexanone 364.2 215.8 126.7 1-Hydroxy-2-propanone 4921.0 2907.7 193.5 2,5-Dimethyl-2-cyclopentenone 22.3 15.4 13.2 2-Cyclopenten-1-one 1782.0 961.9 560.0 2-Methyl-2-cyclopenten-1-one 571.2 312.8 411.9 1-Hydroxy-2-butanone 2509.0 1317.9 469.5 2,3-Dimethyl-2-cyclopenten-1-one 2325.9 1300.1 910.3 3-Methyl-2-cyclopenten-1-one 1475.3 1028.0 1107.0 3-Methyl-2-cyclopenten-1-one 244.2 1351.2 381.7 2,3-Dimethyl-2-cyclopenten-1-one 223.2 106.7 53.1 3,4-Dimethyl-2,5-furandione 1390.1 576.5 30.9 3,4-Dimethylbenzaldehyde 51.85 20.8 - Dihydropyran-3 (4H) -one 147.3 60.1 22.4 2-furfural 4936.3 3423.8 1475.0 1- (2-Furanyl) ethanone 350.5 129.5 82.4 Dihydro-4,5-dimethyl-2H-pyranone 100.1 51.5 95.9 Dihydro-5-methyl-2 (3H) -furanone 1350.7 791.1 499.1 Dihydro-2 (3H) -furanone 214.3 152.7 - Furfuryl alcohol 871.2 197.4 70.3 3-Methyl-2 (5H) -furanone 531.5 292.4 118.0 3,5-Dimethylcyclopentenone 214.2 112.5 109.5 2-Hydroxy-3-methylcyclopentenone 4909.2 3249.9 591.8 Ethylcyclopentenone 743.2 395.5 152.8 5-Methyl-2 (5H) -furanone 600.2 289.3 139.3 4-Hydroxy-2-methyl-4H-pyran-4-one 30.9 16.05 27.2 2-Methoxyphenol (guaiacol) 4086.3 2699.7 2042.4 2-Methoxy-4-methyl phenol 2072.2 1391.4 1023.7 Phenol 6834.0 4554.3 2687.6 4-Ethyl guaiacol 499.2 349.7 407.9 4-Methyl phenol 1709.7 1169.3 631.7 3-Methyl phenol 803.4 421.9 236.3 3-Ethyl phenol 626.5 261.8 167.6 2,6-Dimethoxy phenol (syringol) 1568.1 560.6 692.2 2,3,6-Trimethyl phenol 373.9 115.7 30.4 2-Methoxy-4-methyl phenol 661.2 226.6 8.1 4-Propyl syringol 352.8 136.2 57.1 Vanillin 340.5 193.7 7.6 Acetovanillone 160.1 74.9 - 2,5-Dimethyl toluene 311.3 180.4 68.1 Naphthalene 338.9 16.7 21.7 3,4-Dimethoxy toluene 189.2 107.0 33.6 1,4-Dimethoxy benzene (veratrole) 127.9 63.8 36.3 2-Acetyl pyrrole 51.5 28.3 -

Reference Example  1. Preparation of experimental animals

Six-week-old NC / Nga mice and ICR mice were supplied from the Central Experimental Animal Co., Ltd. and used after undergoing laboratory purification for about one week. Two mice were housed in a cage for mice (220 × 200 × 145 mm). The environment of the animal laboratory was controlled at a temperature of 21-25 ° C., a relative humidity of 45-65%, a frequency of 12 ventilations / hour, a lighting cycle of 12 hours, and an illuminance of 150-300 lux. Purina Rat Chow, a pellet-type solid feed for laboratory animals, was introduced by Nestle Purina PetCare Korea Ltd. It was supplied from (Seoul, Korea) and fed, and the negative water was allowed to take sterile purified water freely.

Reference Example  2. Chaff  Distillate Sample Treatment and Sample Treatment

2.1 Chaff  Distillate Sample DWC -One, DWC -2, DWC -3) kill

In order to measure the allergic reaction inhibitory effect by the chaff herb distillate samples (DWC-1, DWC-2, DWC-3) obtained by the method of Example 2, the experiment was carried out as follows according to the literature (Pokharel et al., Evid Based Complement Alternat Med ., 5 , pp 173-180, 2008).

NC / Nga mice reared by the method of Reference Example 1 and ICR mice as a control group were sufficiently depilated using a depilatory agent (sensitive skin veet), and after 24 hours, a solution containing 3: 1 olive oil and acetone. 150 ul of DNCB prepared at a concentration of 1% was taken and applied at the same time every day for 4 days to induce dermatitis. Thereafter, the DNCB was adjusted to 0.2% in concentrations of 150 ul, and was applied at the same time every other week for 10 weeks simultaneously with rice hull distillate or saline (control). After 10 weeks, fasting for 24 hours before the experiment, the abdomen under light ether anesthesia, blood was collected from the heart, liver and skin tissue was removed, weighed and stored frozen. Extracted skin tissue was added 20 times the weight solution (PBS + 0.1% Tween20) using a homogenizer (homogenizer) for 2 minutes and centrifuged (12,000g, 10 minutes) to obtain a supernatant. The collected blood was centrifuged (3,000 g, 20 minutes) to obtain serum.

Experimental group Treatment details ICR-Normal No treatment ICR-DNCB Kills DNCB kills NC / Nga-Normal No treatment NC / Nga-DNCB Kills DNCB kills NC / Nga-Saline Kill DNCB Kills + Saline Kills NC / Nga-DWC-1 Kills DNCB Kills + DWC-1 Kills NC / Nga-DWC-2 Kills DNCB Kills + DWC-2 Kills NC / Nga-DWC-3 Kills DNCB Kills + DWC-3 Kills

2.2 Manufacturing Cream CR -One, CR -2) kill

In order to measure the allergic reaction inhibitory effect by the manufacturing cream (CR-1) containing no chaff herb distillate prepared by the method of Example 3 and the manufacturing cream (CR-2) containing the chaff herb distillate, Proceed in the same manner as described in Reference Example 2-1, but was performed by treating the experimental animals with CR-1 and CR-2, the product cream.

Experimental group Treatment details ICR-Normal No treatment ICR-DNCB Kills DNCB kills NC / Nga-Normal No treatment NC / Nga-DNCB Kills DNCB kills NC / Nga-vehicle kill DNCB treatment + vehicle (saline) treatment NC / Nga-CR-1 Kill DNCB Treatment + CR-1 Treatment NC / Nga-CR-2 Kills DNCB Treatment + CR-2 Treatment

Reference Example  3. Statistical Analysis

Levene's test was performed to compare the homogeneity of the variances for each experimental result.If the variances were homogeneous, one-way analysis of variance (ANOVA) was observed. Dunnett t- test was performed to identify the test group with statistical differences. It was determined that there was statistical significance when the difference was less than 5% ( p <0.05).

Experimental Example  One. Immunoglobulin  E inhibition effect measurement

Using the mouse IgE ELISA KIT (ALPHA DIAHNOSTIC, USA), each sample obtained from the skin tissue and blood obtained by the method of Reference Example 2-1 was used to measure the change of IgE, a mediator of inflammatory response in serum and skin tissue. It was. 100 μl of sample was placed in an anti-mouse IgE micro well strip plate, and then washed 4 times after being left for 1 hour. Each anti-mouse IgE-HRP conjugate was added to 100ul and left for 30 minutes and washed five times. 100 ul TMB substrate was added to each well, and then photosensitive for 15 minutes, and then 100 ul of stop solution was added and measured by ELISA (Model MCC / 340, Huntsvill, AL, USA) 450 nm.

As a result of the experiment, as shown in Figures 1 and 2, it was confirmed that significant increase in IgE in NC / Nga mice compared to ICR mice in both affected tissue (Fig. 1) and serum (Fig. 2). IgE increased by DNCB treatment in the affected tissues (Fig. 1) was confirmed to significantly reduce the inflammation by chaff distillate (DWC-1, DWC-2 and DWC-3). However, in the serum (FIG. 2), no inhibitory effect by chaff herb distillate (DWC-1, DWC-2 and DWC-3) was observed in IgE increased by DNCB treatment.

Experimental Example  2. Observation of histopathological changes of liver

A portion of the liver obtained from Reference Example 2-1 was sampled and fixed in 10% neutral formalin, followed by dehydration and paraffin embedding, and a longitudinal section of 3 to 4 μm was prepared to stain hematoxylin-eosin (hematoxylin-eosin). The frequency of liver showing abnormal findings was observed per group using an automatic image analysis device (DMI-300 Image Processing; DMI, Korea) in the optical microscope field of view.

Long-term oral administration of DNCB is known to cause hepatic lobular central necrosis (Yamazaki et al., Ind) . Health ., 43 , pp308-319, 2005), it is known that only a slight increase in liver weight is caused by topical application (Arts et al., Food Chem . Toxicol ., 34, pp 55-62, 1996).

In the present study, local inflammatory cell infiltration with necrosis of hepatic parenchymal cells was observed 20.00%, 33.33% and 16.67% in normal NC / Nga mouse group, DWC-1 and DWC-3 treated group, respectively. There was no abnormal findings, and local inflammatory cell infiltration with necrosis of hepatic parenchymal cells was known as one of the rare cases of accidental histological findings in normal mice (Lee et al., J. Toxicol . Pub) . . Health, 22, pp117-126, 2006 ;..... Lee et al, Lab Anim Res, 521, pp299-305, 2005), histopathological changes in the liver by application of DNCB is expected did not happen , Hepatotoxicity was not observed even by treatment with rice hull distillate (DWC-1, DWC-2, DWC-3) (see Fig. 3 and Table 5).

Experimental group Observation of Histopathological Changes in Liver normal Infiltration of inflammatory cells ICR-normal 3/3 (100.00%) 0/3 (0.00%) ICR-DNCB 3/3 (100.00%) 0/3 (0.00%) NC / Nga-normal 4/5 (80.00%) 1/5 (20.00%) NC / Nga-DNCB 5/5 (100.00%) 0/5 (0.00%) NC / Nga-saline 4/6 (100.00%) 0/5 (0.00%) NC / Nga DWC-1 5/6 (83.33%) 2/6 (33.33%) NC / Nga DWC-2 5/6 (83.33%) 1/6 (16.67%) NC / Nga DWC-3 6/6 (100.00%) 0/6 (0.00%)

Experimental Example  3. Observation of histopathological changes of skin tissue

10% by sampling a portion of the skin tissue obtained by the method of Reference Example 2-1 from the experimental animal treated with the rice husk distillate (DWC-1, DWC-2, DWC-3) obtained by the method of Example 2 After fixation to neutral formalin, dehydration and paraffin embedding were performed, longitudinal sections of 3 to 4 μm were prepared, stained with hematoxylin-eosin, the thickness of the entire skin from the epithelium to the dermis, and the skin Epidermis and dermis thickness (um) were measured using an automatic image analysis device (DMI-300 Image Processing; DMI, Korea) at 100 times the microscope field.

DNCB is a representative agent of delayed contact dermatitis. In this experiment, the infiltration of inflammatory cells was relatively weak, whereas the skin thickness was increased due to the thickening of the epidermis.

NC / Nga mice are well known as spontaneous dermatitis-inducing animals (Shiohara et al., J. Dermatol . Sci., 36, pp1-9, 2004). An increased state was observed compared to mice, and a more significant increase in skin thickness was observed by DNCB treatment compared to ICR mice of the same age. ICR mice showed 88.12, 12.78 and 23.74% increase in epithelial, dermal and total skin thickness, respectively, by DNCB treatment, whereas NC / Nga mice showed 289.19, 47.96 and 31.24%, respectively.

As a result of the experiment, the saline treatment of the control group was not found to have a significant effect on DNCB-induced contact dermatitis, but DWC-1 treatment showed -43.65, -22.17 and-total thickness of the epidermis, dermis and skin compared to the control treatment group, respectively. 19.07% reduction, -50.55, -25.91 and -20.40% reduction with DWC-2 treatment, -55.99, -25.59 and -23.29% reduction with DWC-3 treatment, respectively. It was confirmed that the effect was shown (see Fig. 4, Table 6). Therefore, it was confirmed that DWC-1, DWC-2, and DWC-3 of rice hull distillate effectively inhibited contact dermatitis caused by DNCB.

Experimental group thickness epithelium dermis Total skin ICR-normal 43.98 ± 6.15 1034.09 ± 145.33 1130.88 ± 119.57 ICR-DNCB 82.74 ± 4.45 ^A 1166.23 ± 111.20 1399.33 ± 185.85 NC / Nga-normal 67.41 ± 13.42 ^A 829.83 ± 279.84 1095.06 ± 158.22 NC / Nga-DNCB 262.31 ± 90.32 ^BC 1227.78 ± 223.34 ^C 1473.18 ± 80.55 ^C NC / Nga-saline 251.67 ± 49.92 ^C 1331.15 ± 313.87 ^C 1468.85 ± 290.27 ^C NC / Nga DWC-1 141.81 ± 48.75 ^DE 1036.09 ± 256.89 ^F 1188.70 ± 285.57 ^F NC / Nga DWC-2 124.45 ± 40.61 ^DE 986.21 ± 223.28 ^E 1169.21 ± 231.63 ^F NC / Nga DWC-3 110.77 ± 35.92 ^DE 990.46 ± 129.79 ^E 1126.83 ± 138.28 ^E A: p <0.05 compared to ICR-normal; B: p <0.05 compared to ICR-DNCB; P <0.01 compared to C: NC / Nga-normal; P: 0.05 compared to D: NC / Nga-normal; P <0.01 compared to E: NC / Nga-saline; P: 0.05 compared to F: NC / Nga-saline

Experimental Example  4. CR -One, CR -2 Immunoglobulin  E inhibition effect measurement

Reference Example 2- from the experimental animal treated with the product cream (CR-1) not containing the rice husk distillate prepared by the method of Example 3 and the manufacturing cream (CR-2) containing the rice hull distillate IgE, a mediator of inflammatory responses in skin tissue and blood obtained by the method of 2, was measured using mouse IgE ELISA KIT (ALPHA DIAHNOSTIC, USA). 100 μl of sample was placed in an anti-mouse IgE micro well strip plate, and then washed 4 times after being left for 1 hour. Each anti-mouse IgE-HRP conjugate was added to 100ul and left for 30 minutes and washed five times. 100 ul TMB substrate was added to each well, and then photosensitive for 15 minutes, and then 100 ul of stop solution was added and measured by ELISA (Model MCC / 340, Huntsvill, AL, USA) 450 nm.

As a result of the experiment, as shown in Figures 5 and 6, it was confirmed that significant increase in IgE in NC / Nga mice compared to ICR mice in both affected tissue (Fig. 5) and serum (Fig. 6). However, as shown in serum (FIG. 6) and affected tissues (FIG. 5), no significant decrease in IgE by DNCB was observed with treatment of product creams (CR-1, CR-2).

Experimental Example  5. In the liver CR -One, CR Histopathological changes of -2

The product cream (CR-1) containing no chaff herb distillate prepared by the method of Example 3 and the manufacturing cream (CR-2) containing chaff distillate were treated by the method of Reference Example 2-2. A part of the liver of the experimental animal was sampled, fixed in 10% neutral formalin, dehydrated and paraffin embedded, and 3-4 μm longitudial sections were prepared to perform hematoxylin-eosin staining and optical microscopy. The frequency of livers with abnormal findings was observed per group using automatic image analysis (DMI-300 Image Processing; DMI, Korea).

In this study, local inflammatory cell infiltration with necrosis of hepatic parenchymal cells was found to be 20.00% or 16.67% in normal NC / Nga mouse group, product cream-1 (CR-1) and product cream-2 (CR-2) groups, respectively. In spite of sporadic findings, no abnormalities were observed except sporadic findings, and these local inflammatory cell infiltrations were found to be one of the rare incidents observed in normal mice (Lee et al., J Toxicol). Pub Health . 22, pp 117-126, 2006; Lee et al., Lab Anim Res . 521, pp299-305, 2005), it was judged that the histopathological changes of the liver did not occur by the application of DNCB, and liver toxicity was not observed even by treatment with CR-1 and CR-2. 7)

Experimental group Observation of Histopathological Changes in Liver normal Infiltration of inflammatory cells ICR-normal 3/3 (100.00%) 0/3 (0.00%) ICR-DNCB 3/3 (100.00%) 0/3 (0.00%) NC / Nga-normal 4/5 (80.00%) 1/5 (20.00%) NC / Nga-DNCB 5/5 (100.00%) 0/5 (0.00%) NC / Nga-saline 5/5 (100.00%) 0/5 (0.00%) NC / Nga CR-1 4/5 (80.00%) 1/5 (33.33%) NC / Nga CR-2 5/6 (83.33%) 1/6 (0.00%)

Experimental Example  6. In skin tissue CR -One, CR Histopathological changes of -2

Reference Example 2- from the experimental animal treated with the product cream (CR-1) not containing the rice husk distillate prepared by the method of Example 3 and the manufacturing cream (CR-2) containing the rice hull distillate A part of the skin tissue obtained by the method of 2 was sampled and fixed in 10% neutral formalin, followed by dehydration and paraffin embedding, and a longitudinal section of 3 to 4 μm was prepared to stain hematoxylin-eosin. The thickness of the entire skin from the epithelium to the dermis, the thickness of the epidermis and dermis in the skin (um) in a 100-fold microscope view (DMI-300 Image Processing; DMI, Korea) Each was measured using.

DNCB is a representative substance for delayed contact dermatitis. In this experiment, the infiltration of inflammatory cells was relatively weak, whereas the skin thickness was increased due to the thickening of the epidermis.

NC / Nga mice are well known as spontaneous dermatitis-inducing animals (Shiohara et al., J. Dermatol . Sci ., 36 , pp1-9, 2004). An increased state was observed compared to mice, and a more significant increase in skin thickness was observed by DNCB treatment compared to ICR mice of the same age. ICR mice showed 88.12, 12.78 and 23.74% increase in epithelial, dermal and total skin thickness, respectively, by DNCB treatment, whereas NC / Nga mice showed 289.19, 47.96 and 31.24%, respectively.

The control treatment did not significantly affect DNCB-induced contact dermatitis, but the CR-1 treatment showed a reduction of -53.00, -18.39, and -13.66% of epithelial, dermis and skin thickness, respectively, compared to the control treatment. CR-2 treatment showed -58.13, -23.99 and -18.19% reduction effects, respectively (see Figure 8, Table 8). Therefore, it was confirmed that CR-2, a preparation cream containing rice hull distillate, inhibits DNCB-induced contact dermatitis more effectively.

Experimental group thickness epithelium dermis Total skin ICR-Normal (3) 43.98 ± 6.15 1034.09 ± 145.33 1130.88 ± 119.57 ICR-DNCB (3) 82.74 ± 4.45 ^A 1166.23 ± 111.20 1399.33 ± 185.85 NC / Nga-Normal (5) 67.41 ± 13.42 ^A 829.83 ± 279.84 1095.06 ± 158.22 NC / Nga-DNCB (5) 262.31 ± 90.32 ^BC 1227.78 ± 223.34 ^C 1473.18 ± 80.55C NC / Nga-saline (5) 251.67 ± 49.92 ^C 1331.15 ± 313.87 ^C 1468.85 ± 290.27 ^C NC / Nga CR-1 (5) 118.28 ± 21.03 ^CD 1086.41 ± 202.51 1268.26 ± 242.76 NC / Nga CR-2 (6) 105.38 ± 14.17 ^CD 1011.82 ± 226.25 ^E 1201.73 ± 194.74 ^E A: p <0.05 compared to ICR-normal; B: p <0.05 compared to ICR-DNCB; P <0.01 compared to C: NC / Nga-normal; P: 0.05 compared to D: NC / Nga-normal; E: p <0.01 compared to NC / Nga-saline.

As a result, samples containing rice husk distillate (DWC) (DWC-1, DWC-2, DWC-3) and manufactured cream (CR-2) inhibited the production of IgE, the main antibody for allergic reaction, in affected tissues And by confirming the effect of reducing the overall thickness of the epidermis, dermis and skin due to allergic induction without causing liver histopathological changes, the chaff distillate (DWC) has the potential as an allergy treatment and preventive substance. It is considered to have.

Although the formulation example of the composition containing the chaff distillate distillate (DWC) of the present invention will be described, the present invention is not intended to limit this, but is intended to explain in detail only.

Formulation example  One. Powder  Produce

20 mg of rice hull distillate (DWC-3)

Lactose 100 mg

Talc 10 mg

The above ingredients are mixed and filled in an airtight cloth to prepare a powder.

Formulation example  2. Preparation of Tablets

10 mg of rice hull distillate (DWC-3)

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components, tablets are prepared by tableting according to a conventional method for preparing tablets.

Formulation example  3. Manufacture of capsule

10 mg of rice hull distillate (DWC-3)

3 mg of crystalline cellulose

Lactose 14.8 mg

Magnesium Stearate 0.2 mg

According to a conventional capsule preparation method, the above ingredients are mixed and filled into gelatin capsules to prepare capsules.

Formulation example  4. Preparation of Injectables

Rice husk distillate (DWC-3) 10 mg

Mannitol 180 mg

Sterile distilled water for injection 2974 mg

Na ₂ HPO _{4 ,} 12H ₂ O 26 mg

According to the conventional method for preparing an injection, the amount of the above ingredient is prepared per ampoule (2 ml).

Formulation example  5. Liquid  Produce

20 mg of rice hull distillate (DWC-3)

10 g of isomerized sugar

5 g of mannitol

Purified water

According to the conventional method of preparing a liquid solution, each component is added and dissolved in purified water, lemon flavor is added to the mixture, and then the above ingredients are mixed, purified water is added to adjust the total amount to 100 ml, and then filled in a brown bottle. The solution is prepared by sterilization.

Formulation example  6. Manufacture of healthy food

Rice husk distillate (DWC-3) 1000 mg

Vitamin mixture proper amount

70 μg of Vitamin A Acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

Vitamin B6 0.5 mg

0.2 μg of vitamin B12

Vitamin C 10 mg

10 μg biotin

Nicotinic Acid 1.7 mg

50 μg folic acid

Calcium Pantothenate 0.5mg

Mineral mixture

Ferrous Sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium Citrate 90 mg

Calcium Carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixtures is mixed with a component suitable for a health functional food in a preferred embodiment, the composition ratio may be arbitrarily modified, and the above components may be mixed according to a conventional health food manufacturing method. Next, the granules may be prepared and used for preparing a health food composition according to a conventional method.

Formulation example  7. Manufacture of health drinks

Rice husk distillate (DWC-3) 1000 mg

Citric acid 1000 mg

100 g oligosaccharides

Plum concentrate 2 g

1 g of taurine

Add 900 ml of purified water

After mixing the above components according to the conventional healthy beverage production method, and stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilization and then refrigerated and stored Used to prepare the healthy beverage composition of the invention.

Although the composition ratio is a composition that is relatively suitable for the preferred beverage in a preferred embodiment, the compounding ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and usage.

1 is a diagram measuring the effect of chaff distillate (DWC-1, DWC-2, DWC-3) on the production of IgE in the skin of the DNCB-induced contact dermatitis allergic disease model,

2 is a diagram measuring the effect of chaff distillate (DWC-1, DWC-2, DWC-3) on the production of IgE in the serum of the DNCB induced contact dermatitis allergic disease model,

3 is a diagram illustrating the histopathological changes of liver by treatment of DNCB and rice hull distillate (DWC-1, DWC-2, DWC-3), which are contact dermatitis causing agents (a: ICR-normal, b) : ICR-DNCB, c and d: NC / Nga-normal, e: NC / Nga-DNCB, f: vehicle (NC / Nga-saline), g and h: DWC-1, i and j: DWC-2, k: DWC-3),

Figure 4 is a measure of the change in the thickness of the skin tissue by treatment of DNCB and chaff herb distillate (DWC-1, DWC-2, DWC-3) that is a contact dermatitis causing agent (a: ICR-normal, b: ICR-DNCB, c: NC / Nga-normal, d: NC / Nga-DNCB, e: vehicle (NC / Nga-saline), f: DWC-1, g: DWC-2, h: DWC-3 ),

5 is a diagram measuring the effect of the product cream (CR-1, CR-2) on the production of IgE in the skin of the DNCB induced contact dermatitis allergic disease model,

6 is a diagram measuring the effect of the product cream (CR-1, CR-2) on the production of IgE in the serum of the DNCB-induced contact dermatitis allergic disease model,

7 is a diagram illustrating the histopathological changes of the liver by treatment of DNCB and product creams (CR-1 and CR-2), which are the causes of contact dermatitis (a: ICR-normal, b: ICR-DNCB, c). And d: NC / Nga-normal, e: NC / Nga-DNCB, f: vehicle (NC / Nga-saline), g and h: CR-1, i and j: CR-2),

Figure 8 is a measure of the change in the thickness of the skin tissue by treatment of DNCB and product creams (CR-1, CR-2) that causes contact dermatitis (a: ICR-normal, b: ICR-DNCB, c : NC / Nga-normal, d: NC / Nga-DNCB, e: vehicle (NC / Nga-saline), f: CR-1, g: CR-2).

Claims (8)

A first step of carbonizing the chaff at 300 ° C. to 600 ° C. for 40 to 120 minutes; A second step of condensing the smoke discharged during the carbonization process to obtain crude chaff herb distillate; The crude chaff herb distillate of the second step is obtained by the third step of distillation at a low temperature of 40 ℃ ~ 70 ℃, the organic material composition is 40 ~ 70% phenol component fraction, 15 ~ acidic component fraction A pharmaceutical composition for the prevention and treatment of contact dermatitis or atopic dermatitis, containing chaff distillate with 35%, basic fraction of 0.5 to 10%, and neutral component of 10 to 30%. delete delete delete delete delete A first step of carbonizing the chaff at 300 ° C. to 600 ° C. for 40 to 120 minutes; A second step of condensing the smoke discharged during the carbonization process to obtain crude chaff herb distillate; The crude chaff herb distillate of the second step is obtained by the third step of distillation at a low temperature of 40 ℃ ~ 70 ℃, the organic material composition is 40 ~ 70% phenol component fraction, 15 ~ acidic component fraction Health functional food for the prevention and improvement of contact dermatitis or atopic dermatitis, containing chaff distillate with 35%, basic fraction of 0.5 to 10%, neutral fraction of 10 to 30% as an active ingredient. delete

Images