KR100947353B1 - Healthy food composition for improving sleep disturbance and a good-sleep - Google Patents

Abstract

PURPOSE: A health food composition for improving sleep disorders and inducing pleasant sleep is provided to induce the pleasant sleep by relieving or improving the sleep disorder such as insomnia by forming the composition with zizyphi spinosi Semen, taro parts, nelumbinis Semen, lotus root and magnesium. CONSTITUTION: A manufacturing method of a health food composition for improving sleep disorder includes the following steps: mixing 28.5% of roasted Zizyphi Spinosi Semen powder, 24% of dried Nelumbis Semen, 24% of dried Rhizoma Nelumbinis, 22% of dried taro stems and 1.5% of magnesium chloride; mixing a mixture with water; making the mixture to a pill shape; and drying the pill for 16 hours in 75°C.

Description

Healthy food composition for improving sleep disturbance and a good-sleep}

The present invention relates to a health food composition for improving sleep disorders and sleeping comfort, and particularly high in the content of melatonin in the ring composed of Sanjoin, tarodae, lotus root, lotus root and magnesium known to help comfort sleep among natural products showing an excellent sleep disorder improvement effect It relates to a health food composition (hereinafter referred to as "good night" arbitrarily named by the present inventors) for improving sleep disorders and sleeping comfort.

Sleep is a state in which the conscious activity is closed with eyes closed and is an important process of replenishing the energy we used during the day and restoring the fatigue caused by physical activity. Sleep is not only a time to recover energy and fatigue, but also a time when the growth hormone is essential for human growth.

In our body, the brain is responsible for all the physiological functions that maintain life, which requires rest for the brain to balance proper activity.

The number of patients diagnosed and treated for sleep problems has increased in recent years and is expected to increase in the future, due to the exhausting and busy daily pace of modern life, increased prevalence of obesity, and aging population. According to the 2005 National Sleep Foundation survey, nearly three out of 10 adults have been absent from sleep-related problems or made mistakes in their work for the past three months.

Sleep deprivation directly harms your health, and new research shows an increased risk of diabetes, heart disease and obesity. In a 2004 study published in Sleep, women who slept less than five hours a night on average had significantly higher mortality rates than women who slept seven hours.

For insomnia, the administration of sleeping pills, mental stabilizers, stress relief agents and the like are used as a general treatment. However, the effect of improving insomnia is largely dependent on the mental and physical condition of the subject and the surrounding environment, and at the same time, it is limited in terms of side effects and drug dependence, in addition to the characteristics of the action point of the used drug.

For this reason, foreign countries are taking melatonin as a dietary supplement that regulates the sleep cycle. Melatonin is a hormone secreted by the pineal gland in our brain, especially during rhythmic nights. Melatonin is usually synthesized, but is rarely extracted from animal brains. However, taking substances from animal brain tissue also carries a risk of mad cow disease.

Therefore, it is necessary to develop new medicines and functional foods to improve the defects of these sleeping pills, mental stabilizers, stress relief agents, and melatonin on the market.

Therefore, the present invention is to provide a good night for sleeping disorder improvement and sleeping comfort to compensate for the shortcomings of sleeping pills, mental stabilizers, stress relievers, commercial melatonin having various side effects as described above.

Another purpose is to demonstrate functional foods for improving sleep disorders without side effects through good night melatonin level test, the effects of good night on motor neuron and animal experiments of extended time.

If you use it as a means to solve the above problems, it helps to insomnia caused by blood loss, and mixes it with quantitative combinations of Yin-Jin, Lotus Root, Taro, and Magnesium which are known to help insomnia and Sanjoin, which is known to be mainly used in oriental medicine. After developing, reclaiming and drying, Good Night has been developed to help pleasant noodle.

In order to minimize the risk that melatonin can be extracted from animals or synthesized in the human body, only raw materials known to contain large amounts of melatonin are reclaimed from foods commonly used in foods to eliminate the risk of mad cow disease. The use of natural raw materials ensures the stability of the motor function of the human central nervous system.

The present invention has demonstrated high levels of melatonin for healthy foods for improving sleep disorders and sleeping comfort of the present invention, and inducing sleep comfort by relieving and improving sleep disorders such as insomnia, and confirming that the human body such as exercise nerves are improved compared to sleeping pills. The invention was completed.

1.Healthy food composition for improving sleep disorder and sleeping comfort (Goodnight)

-Mix the mixture of 28.5% uncooked roasted acidjoin, dried lotus root meat 24%, dried lotus root 24%, dried taro bar 22%, and magnesium chloride 1.5% in 1: 1 ratio with water. It was dried for 16 hours.

Sanjoin is the shell of the seed of the jujube, and in [Original Oral Medicine] he treats fever, multifaceted, and mental fatigue. If it is used prematurely, it receives the essence and treats insomnia, swelling, and suffocation caused by hull.] In Korean medicine, it is commonly used as a treatment for insomnia and depression. This medicine is roasted and powdered. Park Chung-il, a professor at Seoul National University, said that the roasted Sanjoin ingredients help to calm the rats by making them sleep more than the raw ingredients.

Lotus root and lotus root are the mature seeds of lotus and the root of lotus and are known as foods that help insomnia by calming the heart.

Taro stand is a food that contains a lot of natural melatonin and is known as a food that helps insomnia. Toxic is destroyed when heated, so it is pulverized by heating at 80 ℃ for 6 hours.

Magnesium chloride is a constituent of chlorophyll, a plant pigment, and it is a mineral that can cause mental disorders, anxiety, nervousness, depression, and insomnia when lacking.

2. Manufacturing method of health food composition (good night) for improving sleep disorder and sleeping comfort

Detailed manufacturing method in this regard will be described with reference to FIG.

The present invention provides a method for preparing a health food composition for improving sleep disorders and sleeping comfort. 6) The packing takes place.

1) raw material washing and processing

Sanjoin and lotus root and lotus root and taro stand are washed clean, and Sanjoin is roasted, and lotus root, lotus root and taro stand are dried.

The roasted acid and dried soft meat and taro stand are pulverized using a grinder.

At this time, 60 ~ 70 mesh is suitable for crushed particles.

2) Foreign body sorting (de-iron, de-mineralization)

As described above, iron powder, which can be introduced from a grinder or the like during the grinding process, is degassed using a magnet.

3) Quantitative and Mix

The finely divided raw materials are quantified, and these are put into a stirrer and mixed.

As the raw material, 28.5% of finely ground roasted sanjo, 24% of dried lotus root meat and 24% of dried lotus root, 24% of dried taro, 22% of magnesium chloride were added to the stirrer, and the water was the same as the weight of the mixed raw material. Pour the mixture to mix evenly.

4) Refund

The mixed raw materials quantitatively mixed as described above are returned.

A suitable size of the ring is 2 to 4 mm in diameter.

5) Drying

The refunded goodnight of the present invention is dried for 16 hours using a dryer at 75 ℃. At this time, the moisture content is about 5% or less.

This ensures that there is no risk of deterioration in the distribution process.

6) Packing

Dry refills are packed in containers or packs.

Good night consisting of the active ingredients of each raw material has the side effects of using the current commercially available sleeping pills as a single ingredient, it is possible to simply ingest and to reduce the symptoms of insomnia without side effects and help sleep.

As a result of measuring the amount of melatonin necessary for sleep, it contained 2.94% of the total content of melatonin, which proved to be effective in prolonging the sleep time. Experiment 1

The rats were compared with lorazepam (a drug used as a tranquilizer) to observe the effects on motor function and confirmed no side effects. Experiment 2

Sleep time extension experiments using rats also confirmed a clear sleep time extension when ingested Goodnight. Experiment 3

Tests on reactions to good night's intake in people with sleep problems were found to be beneficial to sleep.

Experiment 1 Measurement of Melatonin Content

1. Test Method

(1) Melatonin Extraction from Good Night: Filtered after centrifugation (5000rpm, 5min) and ultrasound (15min) extraction using ethanol (HPLC grade). (Reference, Melatonin Determination using HPLC: Yongin University, 2001, 19, 2001 -Don Chan Choi, Temporary)

(2) Analyzer: HPLC (Agilent1200)

              Column: C18

              Mobile phase: KH 2 PO 4: MeOH = 60: 40, 0.8 ml / min

              Detector: UV, 200nm

(3) Standard solution: Dissolve melatonin standard (100% powder) in ethanol to prepare 10%, 20%, 30% solution.

(4) Sample Solution Preparation

 Make Goodnight 10% solution in 100 ml of ethanol, and concentrate it in vacuo to make 18.7 ml.

2. Test result

Melatonin peak at Goodnight was measured to be 15.668%.

Since the sample solution was concentrated to 18.7 / 100, the melatonin content in the goodnight was measured as 2.92992%. (Details are shown in Figs. 2 and 3).

<Experiment 2> Effect on exercise function

1. Test method.

As a test animal, male ICR mice with a body weight of 20 ± 2 g and 4 to 5 weeks of age were purchased from Hyochang Science (company in Daegu).

After crushing the sample 'goodnight' to powder, 540g of powder was extracted three times with methanol for 5 hours to obtain 54g (10%) of the extract. This methanol extract was dissolved in a food emulsifying agent (trade name; tween80) (tween80: saline (saline) (1: 4)) at a dose of 5, 10, or 20 mg / kg. Lorazepam was used as a control drug.

(1) suspension

Five mice in a group were selected to select mice that could hang for more than 5 seconds with their forelimbs on a horizontal 3mm diameter metal rod. After 15 minutes, 30 minutes, 60 minutes and 120 minutes of oral administration of Goodnight Methanol X, it was determined that no action was allowed to be suspended on a metal rod for more than 5 seconds.

(2) inclined plate method

Mice that could be stationary for more than 5 seconds on a 40 degree inclined glass plate were selected and tested in 5 groups. After 15 minutes, 30 minutes, 60 minutes and 120 minutes of oral administration of Goodnight Methanol X, it was determined that no action was allowed to be placed on an inclined glass plate for more than 5 seconds.

(3) Rota-rod method

Using mice, the animals were placed on the jeonbong (3 cm in diameter, 6 rotations per minute), and the animals that did not fall for more than 2 minutes were selected as 5 groups of 1 group. After 15 minutes, 30 minutes, 60 minutes and 120 minutes of oral administration of Goodnight Methanol X, the time until the drop is placed on the rotating rod and measured. Again, it was judged that there was no action when it did not fall more than 4 minutes.

2. Experimental Results

(1) suspension

Observations of visual placing and holding reflex, one of the innate reflexes, showed that the reflex was hardly inhibited by the Goodnight methanol extract. On the other hand, the concentration of lorazzepam (lorazepam) (3, 5 ㎎ / ㎏) was suppressed their reflex (reflection), but was found to recover after time. (Table 1, Table 2)

Table 1. Precise positioning results for rats receiving vehicle (feed only), goodnight and lorazepam

Table 2. Results of Catch Reflexes in Mice Treated with Vehicle (Good Feed Only), Goodnight, and Lorazepam

(2) sloped plate method

Even after the oral administration of Goodnight methanol extract, it was shown to be suppressed at the beginning of the experiment with Goodnight methanol extract 10, 20mg / kg, but recovered almost at 120 minutes. However, in the gradient plate test of lorazepam, reflex was significantly inhibited and the inhibition time lasted a long time. Table 3

Table 3. Results of gradient plate tests in rats receiving vehicle (feed only), goodnight and lorazepam

(3) rotating rod method

Good night methanol extracts were orally administered and the rota-rod test (rotary rod test) showed no significant difference from the control group. Lorazepam, on the other hand, significantly reduced the treadmill response at high doses (Table 4).

Table 4. Results of a spinning rod test in rats receiving vehicle (feed only), goodnight and lorazepam

<Experiment 3> Effects on Sleep Time

1. Experimental method

As the experimental animals, male ICR mice of 20 to 2 g body weight and 4 to 5 weeks of age were purchased from Hyochang Science (Daegu). After crushing the sample 'goodnight' to powder, 540g of powder was extracted three times with methanol for 5 hours to obtain 54g (10%) of the extract. This methanol extract was dissolved in a food emulsifying agent (trade name; tween80) (tween80: saline (saline) (1: 4)) at a dose of 5, 10, or 20 mg / kg. Lorazepam was used as a control drug.

(1) single dose

Mice were experimented with 5 mice per group. After 15 minutes of lorazepam, oral administration of Goodnight Methanol X, 30 minutes after injection of phentobarbitad sodium (30 mg / kg), the righting reflex (reflection reflection: rats in supine position) In this case, the time from disappearance to recovery was measured while raising the upper body.

(2) repeated administration

Mice were experimented with 5 mice per group. Good night methanol extract (5, 10, 20 mg / kg) and lorazepam (0.025, 0.05, 0.1 mg / kg) were administered orally for 1 week, 30 minutes after the test, 30 mg / kg of phentobarbital sodium (pentobabital) was intraperitoneally injected. We measured the time from loss of righting reflex (turning the body while raising the upper body when the rat was in the supine position) to recovery.

2. Experimental Results

(1) Single dose

Sleeping time by phentobarbital sodium tended to increase slightly compared to the control group administered orally goodnight methanol extract (Table 5).

Table 5. Sleep time results in rats treated with vehicle (control), goodnight and lorazepam

Mice were intraperitoneally injected with penitebarbital (30 mg / kg) after 30 minutes of oral administration of Goodnight and Lorazepam ethanol extracts. Values were expressed as mean ± standard deviation after five experiments. Duncan's new multiple test showed significant differences (p <0.05).

(2) repeated dose effect

Sleeping time tended to increase somewhat compared to the single-dose administration of goodnight methanol extract and lorazepam for 1 week before oral administration of phentobarbital sodium (Table 6).

Table 6. Sleep time results in rats treated with vehicle (control), goodnight, and lorazepam

Mice were injected orally once a day with ethanol extracts of goodnight and lorazepam, and repeatedly administered for 1 week, and dissolved in pentobarbital (30 mg / kg) 30 minutes after the final sample was intraperitoneally injected. Values were expressed as mean ± standard deviation after five experiments. The data showed significant differences with Duncan's new multiple test (p <0.05).

<Experiment 4> Improvement of sleep quality according to good night intake

1. Test Method

(1) Test sample: Goodnight (sanjoin 28.5%, lotus root 24%, lotus root 24%, taro 22%, magnesium chloride 1.5%)

(2) Number of subjects: 91 (20s-1 people, 30s-1 people, 40s-17 people, 50s-32 people, 60s-30 people, 70s-10 people)

(3) Test period: Intake period is taken at least twice a day for more than one month (30 days).

(4) Intake Method: Insert 1 tablespoon (4.5g) with water.

(5) Test subject

＊ Selection criteria

-① Age 20 years or older

-② Those who have trouble sleeping

＊ Exclusion Criteria

-① As a result of the background check, the person judged that there is no problem with sleep

-② Pregnant, pregnant woman who is pregnant, lactating wife

-③ People with severe hay fever, gastrointestinal disease, liver disease, heart disease and heart disease

④ Those who cannot stop the medicine (including cold medicine, anti-allergic medicine, etc.) and supplements that affect sleep during the trial period

＊ Investigation item

① Background Check: Write your age, gender, lifestyle, and sleep status during the observation period.

-② Sleep investigation: Write the quality of sleep and refreshment when waking up before and after intake.

2. Test result

(1) Improve sleep disorders Improve sleep quality according to good night intake 1.

As can be seen from the above results, it was confirmed that after ingestion of goodnight, sleep before bedtime was significantly improved.

(2) Improve sleep disorders Improve sleep quality according to good night intake 2.

As can be seen from the above results, it was easy to open the eyes in the morning after sleep after ingesting goodnight, and it was confirmed that the freshness was felt after sleep.

(3) Improve sleep disorders Improve sleep quality according to good night intake 3.

As can be seen from the above results, good night's sleep after ingesting good night was 58% or more, compared to 2% before intake.

(4) Improve sleep disorders Improve sleep quality according to good night intake 4.

As can be seen from the above results, after ingesting goodnight, it was possible to take a good night's sleep without dreaming.

Through the above experiment, after taking good night, it was confirmed that the effect of each component of the melatonin contained in the good night, as well as taking a good night's sleep.

1 is a block diagram showing a process of the preferred manufacturing method proposed in the present invention

Figure 2 is a graph of melatonin analysis using HPLC-Calibration

3 is a graph of melatonin analysis using HPLC-melatonin analysis value

Claims (2)

A health food composition for improving sleep disorders and sleeping comfort; A mixture of 28.5% uncooked roasted acidjoin, dried lotus root meat 24%, dried lotus root 24%, dried taro barley 22%, and magnesium chloride 1.5% was mixed in a 1: 1 ratio with water. Health food composition for improving sleep disorders and sleeping comfort, characterized in that dried over time. delete

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