KR100935826B1 - 부유밀도구배 원심분리를 이용하여 말초혈액단핵구로부터 단세포를 분리하는 방법 - Google Patents
부유밀도구배 원심분리를 이용하여 말초혈액단핵구로부터 단세포를 분리하는 방법 Download PDFInfo
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- KR100935826B1 KR100935826B1 KR1020070121459A KR20070121459A KR100935826B1 KR 100935826 B1 KR100935826 B1 KR 100935826B1 KR 1020070121459 A KR1020070121459 A KR 1020070121459A KR 20070121459 A KR20070121459 A KR 20070121459A KR 100935826 B1 KR100935826 B1 KR 100935826B1
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- cells
- density gradient
- gradient centrifugation
- single cells
- peripheral blood
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
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Abstract
Description
분리방법 | 플라스크 부착법 | OptiPrep 이용 방법 | Percoll 이용 방법 | 부유밀도구배법 (1.072 g/ml-335 Osm/L H2O) |
순 도 | - | 87.9 ~ 96.4% | 75% | 75% |
회수율(수율) | - | 26.1% | 13.5% | 33% |
장·단점 | 고전적인 방법으로 시간 및 노동 집약적이며,연구자의 숙련도에 따라 편차가 심함. | OptiPrep 용액을 이용하여 비중 및 삼투압을 조절하기위한 시약조제 과정이 복잡함.(5단계) | hyper- 및 iso-osmotic Percoll 용액 조제 과정이 필요하며 2번의 밀도구배 원심분리가 요구됨. | 비중 및 삼투압 조절을 위한 시약 조제가 간단하며 1번의 밀도구배 원심분리로 단세포를 분리할 수 있다. |
Claims (6)
- 삭제
- 삭제
- 히스토페이크(Histopaque) 또는 피콜(ficoll) 용액을 이용하여 말초혈액단핵구(PBMC)를 부유밀도 구배 원심분리함으로써 단세포를 분리하는 방법에 있어서,히스토페이크(Histopaque)-1077 또는 피콜(ficoll) 용액으로서 밀도와 삼투압 농도가 각각 1.072g/ml 및 335 Osm/L H2O인 것을 사용하는 것을 특징으로 하는,부유밀도구배 원심분리를 이용하여 말초혈액단핵구로부터 단세포를 분리하는 방법.
- 제3항에 있어서,히스토페이크(Histopaque)-1077 또는 피콜(ficoll) 용액의 밀도와 삼투압 농도를 그 정해진 수치로 하기 위한 수단으로서 히스토페이크(Histopaque)-1077 또는 피콜(ficoll) 용액에 인산염완충액(Phosphate Buffered Saline, PBS)과 NaCl 용액을 혼합하는 것을 특징으로 하는,부유밀도구배 원심분리를 이용하여 말초혈액단핵구로부터 단세포를 분리하는 방법.
- 삭제
- 삭제
Priority Applications (1)
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KR1020070121459A KR100935826B1 (ko) | 2007-11-27 | 2007-11-27 | 부유밀도구배 원심분리를 이용하여 말초혈액단핵구로부터 단세포를 분리하는 방법 |
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KR1020070121459A KR100935826B1 (ko) | 2007-11-27 | 2007-11-27 | 부유밀도구배 원심분리를 이용하여 말초혈액단핵구로부터 단세포를 분리하는 방법 |
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KR20090054666A KR20090054666A (ko) | 2009-06-01 |
KR100935826B1 true KR100935826B1 (ko) | 2010-01-08 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20210107335A (ko) | 2020-02-24 | 2021-09-01 | 주식회사 싸이토딕스 | 분리 챔버, 이를 포함하는 ctc 분리 장치 및 ctc 분리 방법 |
KR20220033196A (ko) | 2020-09-09 | 2022-03-16 | 주식회사 싸이토딕스 | 원심 분리용 챔버 및 이를 이용한 원심 분리 방법 |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR102302776B1 (ko) * | 2019-10-11 | 2021-09-23 | 주식회사 싸이토딕스 | 광경화성 하이드로겔을 이용한 pbmc 분리 디스크 |
KR102536272B1 (ko) | 2021-01-29 | 2023-05-24 | 주식회사 큐리오시스 | 용액분리기를 포함하는 원심분리 튜브 및 이를 이용한 혼합 용액의 분리 방법 |
KR20220135994A (ko) * | 2021-03-31 | 2022-10-07 | 주식회사 씨티셀즈 | 자연 살해 세포 분리 장치 및 그의 자연 살해 세포 분리 방법 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2006127150A2 (en) | 2005-04-08 | 2006-11-30 | Argos Therapeutics, Inc. | Dendritic cell compositions and methods |
US20070259330A1 (en) * | 2006-05-04 | 2007-11-08 | Ge Healthcare Bio-Sciences Ab | Separation of cells |
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2006127150A2 (en) | 2005-04-08 | 2006-11-30 | Argos Therapeutics, Inc. | Dendritic cell compositions and methods |
US20070259330A1 (en) * | 2006-05-04 | 2007-11-08 | Ge Healthcare Bio-Sciences Ab | Separation of cells |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20210107335A (ko) | 2020-02-24 | 2021-09-01 | 주식회사 싸이토딕스 | 분리 챔버, 이를 포함하는 ctc 분리 장치 및 ctc 분리 방법 |
KR20220033196A (ko) | 2020-09-09 | 2022-03-16 | 주식회사 싸이토딕스 | 원심 분리용 챔버 및 이를 이용한 원심 분리 방법 |
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